MD15G1167400.v1.1

Rap1-interacting factor 1 N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006378
Physical Location & Seq
Reverse (-)
12668627 .. 12670640
2014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1167400.v1.1.491

Sequence Viewer

Length: 984 bp
ATGTCCGGATTTTCAGACCAGCTCGAAGAAATCAAATCCCTGATATCTTCAGCCGCCACCAAAGCAAACAAGTCCTTCGCCTACTCCACCCTCTTACACCTCCAGCAGCAATCCTCCGACTGCGACGATTCAATTCAAATGCTTGCGCGGAGCTGCCGGAGCCTCATCCGTCCGACCGTCGCCGATGTTCGGAACAATGACGAAGAAATTGCAACTCAGGCATTGAAATGCTTGGGCTTTATGATATATCATCCGTCCATCGTTGCCGAAATCGCAGCGGATGATGTCAAATTTGTTTTAGATTCACTGGCAAAGCTCATTACAACCACAAAAATGAAGTCTGTTTGCAATTTAGGGGTGTGGTGCATATCTATCCAACAATTGAGTACACCACTTTTAGCTGCCCATTTCCATTATCTTTTGCTTGCGATTGTTCATGCCATTGACAATCCTATCGGTTCTTTGTCGACTACTTTTGAGGCTATTCAGGCTGTTATGAAGCTGGCAACTTTGTTAAGTGAGAATATGAGAGAGATGTCGCATATATGGGCTCCTCCAGTATATAGAAGACTCCTTAGCTCTGATAAGAGGGAGAGGGATATGTCGGAGAGTTGTTTATTGAACGCTAGGTCTACAATATTACCCCCTCCGCTAAATCTTTCCAAGGCAGTTGTCAAAGATTTGAAGCCAAGGTTGCTTACTGGGATGCACCACATGCTAACCAATGGCATGAAGGTTCAAACTATTCAAGCATGGGGATGGTTTATCCGCTTACTAGGATCTCATGCGTTGAAGAACAGGCAGTTAATTAATCAGATGCTAAAAATTCCCGAGCAGACATTTTCAGATCATGACTCACAAGTTCAAATTGCTTCACAAGTTGCCTGGGAAGGTCTTATTGAGAATGCAGAGTGCAGAGTGCATCTTGGAGCTGTTGAGAAAGGGATAGTTTGGCTGTCTCAGTACTCTCAGCTTCTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04550 map04550
Pfam Domains
Protein Families
Rif1_N

Protein Analysis

328

Amino Acids

36.54

Weight (kDa)

7.67

Isoelectric Point (pI)

47.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rif1_N PF12231 24 - 300 7.6e-35 Rap1-interacting factor 1 N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 467, 632
AccII CGCG 1 cut(s) 148
AccIII TCCGGA 1 cut(s) 5
AciI CCGC 5 cut(s) 54, 148, 278, 650, 769
AclWI GGATC 1 cut(s) 787
AcsI RAATTY 2 cut(s) 290, 825
AcuI CTGAAG 1 cut(s) 33
AfaI GTAC 2 cut(s) 388, 965
AfiI CCNNNNNNNGG 1 cut(s) 189
AgsI TTSAA 9 cut(s) 132, 137, 226, 622, 685, 740, 749, 793, 866
AjnI CCWGG 1 cut(s) 884
AluBI AGCT 8 cut(s) 22, 153, 316, 401, 502, 579, 932, 973
AluI AGCT 8 cut(s) 22, 153, 316, 401, 502, 579, 932, 973
Alw26I GTCTC 1 cut(s) 963
AlwI GGATC 1 cut(s) 787
Ama87I CYCGRG 1 cut(s) 830
Aor13HI TCCGGA 1 cut(s) 5
ApeKI GCWGC 4 cut(s) 106, 153, 275, 401
ApoI RAATTY 2 cut(s) 290, 825
ArsI GACNNNNNNTTYG 2 cut(s) 323, 355
AseI ATTAAT 1 cut(s) 810
AspLEI GCGC 1 cut(s) 148
AvaI CYCGRG 1 cut(s) 830
BanII GRGCYC 1 cut(s) 553
BbsI GAAGAC 1 cut(s) 574
BbvI GCAGC 4 cut(s) 118, 140, 287, 388
BccI CCATC 2 cut(s) 266, 753
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BciT130I CCWGG 1 cut(s) 886
BcoDI GTCTC 1 cut(s) 963
BfaI CTAG 2 cut(s) 627, 776
BisI GCNGC 5 cut(s) 54, 107, 154, 276, 402
BlsI GCNGC 5 cut(s) 55, 108, 155, 277, 403
BmcAI AGTACT 1 cut(s) 965
Bme1390I CCNGG 1 cut(s) 886
BmeT110I CYCGRG 1 cut(s) 830
BmiI GGNNCC 2 cut(s) 161, 552
BmrFI CCNGG 1 cut(s) 886
BmrI ACTGGG 1 cut(s) 711
BmsI GCATC 3 cut(s) 696, 807, 931
BmuI ACTGGG 1 cut(s) 711
BpiI GAAGAC 1 cut(s) 574
BpmI CTGGAG 2 cut(s) 86, 540
Bpu10I CCTNAGC 1 cut(s) 575
BsaJI CCNNGG 3 cut(s) 663, 689, 885
BsaWI WCCGGW 1 cut(s) 5
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse1I ACTGG 3 cut(s) 312, 557, 706
BseAI TCCGGA 1 cut(s) 5
BseBI CCWGG 1 cut(s) 886
BseDI CCNNGG 3 cut(s) 663, 689, 885
BseGI GGATG 5 cut(s) 165, 250, 286, 711, 764
BseLI CCNNNNNNNGG 1 cut(s) 189
BseMII CTCAG 3 cut(s) 230, 974, 983
BseNI ACTGG 3 cut(s) 312, 557, 706
BseRI GAGGAG 1 cut(s) 543
BseXI GCAGC 4 cut(s) 118, 140, 287, 388
BsgI GTGCAG 1 cut(s) 934
Bsh1236I CGCG 1 cut(s) 148
Bsh1285I CGRYCG 1 cut(s) 177
BsiEI CGRYCG 1 cut(s) 177
BsiHKCI CYCGRG 1 cut(s) 830
BsiSI CCGG 2 cut(s) 6, 157
BslI CCNNNNNNNGG 1 cut(s) 189
BsmAI GTCTC 1 cut(s) 963
BsmI GAATGC 1 cut(s) 910
BsoBI CYCGRG 1 cut(s) 830
Bsp1286I GDGCHC 1 cut(s) 553
Bsp13I TCCGGA 1 cut(s) 5
Bsp143I GATC 2 cut(s) 779, 847
BspACI CCGC 5 cut(s) 54, 148, 278, 650, 769
BspCNI CTCAG 3 cut(s) 229, 973, 982
BspEI TCCGGA 1 cut(s) 5
BspFNI CGCG 1 cut(s) 148
BspHI TCATGA 1 cut(s) 850
BspLI GGNNCC 2 cut(s) 161, 552
BspPI GGATC 1 cut(s) 787
BsrI ACTGG 3 cut(s) 312, 557, 706
BssECI CCNNGG 3 cut(s) 663, 689, 885
BssMI GATC 2 cut(s) 779, 847
BssT1I CCWWGG 2 cut(s) 663, 689
Bst2UI CCWGG 1 cut(s) 886
Bst4CI ACNGT 1 cut(s) 178
BstAPI GCANNNNNTGC 1 cut(s) 715
BstC8I GCNNGC 3 cut(s) 144, 426, 504
BstDEI CTNAG 4 cut(s) 216, 575, 960, 969
BstF5I GGATG 5 cut(s) 165, 250, 286, 711, 764
BstFNI CGCG 1 cut(s) 148
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 2 cut(s) 782, 850
BstMAI GTCTC 1 cut(s) 963
BstMBI GATC 2 cut(s) 779, 847
BstMCI CGRYCG 1 cut(s) 177
BstMWI GCNNNNNNNGC 7 cut(s) 62, 159, 218, 272, 488, 694, 715
BstNI CCWGG 1 cut(s) 886
BstNSI RCATGY 1 cut(s) 718
BstSCI CCNGG 1 cut(s) 884
BstUI CGCG 1 cut(s) 148
BstV1I GCAGC 4 cut(s) 118, 140, 287, 388
BstV2I GAAGAC 1 cut(s) 574
BstX2I RGATCY 1 cut(s) 779
BstYI RGATCY 1 cut(s) 779
BtsCI GGATG 5 cut(s) 165, 250, 286, 711, 764
BtsIMutI CAGTG 1 cut(s) 305
Cac8I GCNNGC 3 cut(s) 144, 426, 504
CciI TCATGA 1 cut(s) 850
CfoI GCGC 1 cut(s) 148
Csp6I GTAC 2 cut(s) 387, 964
CviAII CATG 6 cut(s) 437, 715, 730, 753, 785, 851
CviQI GTAC 2 cut(s) 387, 964
DdeI CTNAG 4 cut(s) 216, 575, 960, 969
DpnI GATC 2 cut(s) 781, 849
DpnII GATC 2 cut(s) 779, 847
Eco130I CCWWGG 2 cut(s) 663, 689
Eco24I GRGCYC 1 cut(s) 553
Eco32I GATATC 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 33
Eco88I CYCGRG 1 cut(s) 830
EcoRII CCWGG 1 cut(s) 884
EcoRV GATATC 1 cut(s) 45
EcoT14I CCWWGG 2 cut(s) 663, 689
EcoT38I GRGCYC 1 cut(s) 553
ErhI CCWWGG 2 cut(s) 663, 689
FaeI CATG 6 cut(s) 440, 718, 733, 756, 788, 854
FatI CATG 6 cut(s) 436, 714, 729, 752, 784, 850
FblI GTMKAC 2 cut(s) 467, 632
Fnu4HI GCNGC 5 cut(s) 54, 107, 154, 276, 402
FokI GGATG 5 cut(s) 152, 237, 293, 718, 771
FriOI GRGCYC 1 cut(s) 553
Fsp4HI GCNGC 5 cut(s) 54, 107, 154, 276, 402
FspBI CTAG 2 cut(s) 627, 776
GlaI GCGC 1 cut(s) 147
GluI GCNGC 5 cut(s) 54, 107, 154, 276, 402
GsuI CTGGAG 2 cut(s) 86, 540
HapII CCGG 2 cut(s) 6, 157
HhaI GCGC 1 cut(s) 148
Hin1II CATG 6 cut(s) 440, 718, 733, 756, 788, 854
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HinfI GANTC 4 cut(s) 128, 302, 570, 854
HpaII CCGG 2 cut(s) 6, 157
Hpy166II GTNNAC 3 cut(s) 389, 468, 633
Hpy188I TCNGA 8 cut(s) 16, 118, 174, 192, 583, 607, 816, 847
Hpy188III TCNNGA 3 cut(s) 6, 830, 851
Hpy8I GTNNAC 3 cut(s) 389, 468, 633
Hpy99I CGWCG 2 cut(s) 128, 182
HpyAV CCTTC 3 cut(s) 85, 727, 884
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4V TGCA 7 cut(s) 212, 348, 366, 709, 908, 915, 922
HpyF10VI GCNNNNNNNGC 7 cut(s) 62, 159, 218, 272, 488, 694, 715
HpyF3I CTNAG 4 cut(s) 216, 575, 960, 969
Hsp92II CATG 6 cut(s) 440, 718, 733, 756, 788, 854
HspAI GCGC 1 cut(s) 146
Kpn2I TCCGGA 1 cut(s) 5
Kzo9I GATC 2 cut(s) 779, 847
LmnI GCTCC 4 cut(s) 150, 159, 556, 929
Lsp1109I GCAGC 4 cut(s) 118, 140, 287, 388
LweI GCATC 3 cut(s) 696, 807, 931
MaeI CTAG 2 cut(s) 627, 776
MalI GATC 2 cut(s) 781, 849
MboI GATC 2 cut(s) 779, 847
MboII GAAGA 5 cut(s) 38, 39, 215, 579, 805
MfeI CAATTG 1 cut(s) 380
MflI RGATCY 1 cut(s) 779
MhlI GDGCHC 1 cut(s) 553
MluCI AATT 8 cut(s) 132, 207, 290, 349, 380, 807, 825, 867
MlyI GAGTC 2 cut(s) 564, 848
MmeI TCCRAC 4 cut(s) 141, 197, 400, 585
MnlI CCTC 9 cut(s) 101, 110, 124, 173, 472, 564, 582, 588, 657
MroI TCCGGA 1 cut(s) 5
MseI TTAA 3 cut(s) 515, 806, 810
MslI CAYNNNNRTG 3 cut(s) 226, 332, 757
MspA1I CMGCKG 1 cut(s) 278
MspI CCGG 2 cut(s) 6, 157
MspR9I CCNGG 1 cut(s) 886
MunI CAATTG 1 cut(s) 380
Mva1269I GAATGC 1 cut(s) 910
MvaI CCWGG 1 cut(s) 886
MvnI CGCG 1 cut(s) 148
MwoI GCNNNNNNNGC 7 cut(s) 62, 159, 218, 272, 488, 694, 715
NdeII GATC 2 cut(s) 779, 847
NlaIII CATG 6 cut(s) 440, 718, 733, 756, 788, 854
NlaIV GGNNCC 2 cut(s) 161, 552
NspI RCATGY 1 cut(s) 718
PacI TTAATTAA 1 cut(s) 810
PagI TCATGA 1 cut(s) 850
PctI GAATGC 1 cut(s) 910
PfeI GAWTC 2 cut(s) 128, 302
PkrI GCNGC 5 cut(s) 55, 108, 155, 277, 403
PleI GAGTC 2 cut(s) 564, 848
PpsI GAGTC 2 cut(s) 564, 848
PshBI ATTAAT 1 cut(s) 810
Psp6I CCWGG 1 cut(s) 884
PspGI CCWGG 1 cut(s) 884
PspN4I GGNNCC 2 cut(s) 161, 552
PsuI RGATCY 1 cut(s) 779
RsaI GTAC 2 cut(s) 388, 965
RsaNI GTAC 2 cut(s) 387, 964
RseI CAYNNNNRTG 3 cut(s) 226, 332, 757
SalI GTCGAC 1 cut(s) 466
SaqAI TTAA 3 cut(s) 515, 806, 810
SatI GCNGC 5 cut(s) 54, 107, 154, 276, 402
Sau3AI GATC 2 cut(s) 779, 847
ScaI AGTACT 1 cut(s) 965
SchI GAGTC 2 cut(s) 564, 848
ScrFI CCNGG 1 cut(s) 886
SduI GDGCHC 1 cut(s) 553
SfaNI GCATC 3 cut(s) 696, 807, 931
SmiMI CAYNNNNRTG 3 cut(s) 226, 332, 757
Sse9I AATT 8 cut(s) 132, 207, 290, 349, 380, 807, 825, 867
SsiI CCGC 5 cut(s) 54, 148, 278, 650, 769
SspI AATATT 1 cut(s) 639
SspMI CTAG 2 cut(s) 627, 776
StyD4I CCNGG 1 cut(s) 884
StyI CCWWGG 2 cut(s) 663, 689
TaaI ACNGT 1 cut(s) 178
TaqI TCGA 2 cut(s) 24, 467
TasI AATT 8 cut(s) 132, 207, 290, 349, 380, 807, 825, 867
TatI WGTACW 2 cut(s) 386, 963
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 2 cut(s) 128, 302
Tru1I TTAA 3 cut(s) 515, 806, 810
Tru9I TTAA 3 cut(s) 515, 806, 810
TscAI CASTG 1 cut(s) 312
TseI GCWGC 4 cut(s) 106, 153, 275, 401
TspDTI ATGAA 4 cut(s) 350, 425, 512, 746
TspGWI ACGGA 2 cut(s) 158, 243
TspRI CASTG 1 cut(s) 312
VspI ATTAAT 1 cut(s) 810
XapI RAATTY 2 cut(s) 290, 825
XceI RCATGY 1 cut(s) 718
XmiI GTMKAC 2 cut(s) 467, 632
XspI CTAG 2 cut(s) 627, 776
ZrmI AGTACT 1 cut(s) 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.