RH2BG030500

Rap1-interacting factor 1 N terminal

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0006378
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG030500.1

Sequence Viewer

Length: 3432 bp
ATGTCGAATCTCTCAGACCAGCTCGAAGAAATCAAATCCCTAATTTCTTCACCCACCAAATCAAACAAGTCACTCGCTTACTCGACTCTATCACACCTCCAACAACAGTCCTCCGATGCTCTGATTCAAACACTCGCGCTGAGCTCTCAGAGCCTTCTCCGTCGGATCGTCGCCGATATTCACGACGACGACGAAGAAATTGCGGCGCAGGCATTGAAATGTTTGGGTTTTATGATATACCATCCCGCCATTGTTTCCGAAATTTCAGTGGAGGATGTGAAGGTGGTTTTGGAATCATTGGTTAAACTCATTACAACTACGAAAATTAAGTCGGTTTGTAATTTAGGAGTGTGGTGCATATCTATACAACAGCTGAGTGAACCACTTTTGGCTCCCCATTTCCATTCTCTTTTGCGCGCGGTAGTTCATGCCATTGACAATCCCATTGGTTCTCTGTCAACAACTTTTGAGGCTATTCAGGCTGTTATTAAGCTAGCAACTCTTTTAAGTGAGAACATGAGAGAGTTATCTCATATATGGGCACCTCCAATATATAGAAGACTGCTTAGCTCTGATAAGAGAGACAGGGATATGTCAGAAAGGTGTCTGTTGAAGATTAGATCCACAATCATACCCCCTCCAATAGATCTCTCCAAGGTACTTGTCAGAGATTTAAAGACAAACTTGCTCACTATGATGCACGATATGCTAACCAATGGTATGAAGGTTCAAACTATTCAAGCATGGGGATGGTATATCCGCTTACTAGGGTCTCATGCCTTGAAGAACAGACATTTAATTAATGATATGCTGAAAATTCCCGAGAAAACATTTTCAGATCACGACTCACAAGTTCAGATTGCTTCCCAGGTTGCCTGGGAAGGTCTTATTGATGCTCTTATTCACCCTCCAATGATATTACCGTGTGCAACAAATGCTGCAAAAGAGGATGATGGTACGCAAAAAAAGGGAACACATAAAGGAAACAGTGGTGACATCCAAAACACTGGATCTTTGAAAAGCATAAGGCTGATCATGACACCACTGATAGGCATCATGTTGAATAACTGTGATGTATCTGTCCACATGGCTTGCTTGAACACGTGGTGCTATCTATTACATAGACTTGATACCTCTCTGAATGATTCTTCAATGATCAACCTGGTAGTAGACCCTATCTTTGAAGCAGCTTTTCAACTTGATCCTGATGGTAAGAGCTTCTGGGTTAGGGATCTGTGCATTAATTTGCTTGATGATTTTATATCAGCAAAATTTAAACCTCAAGACTATGACCCACGTAACCAGGTAAGCCAGCAGTTATCAGCCAAAACCTCTATGAATGCTCCCATTATATCTGGCAGTTGCTCATGGAAGCAGTATCCTATTAAATGGTTGCCATGGGATTTAAGTCTGTTGGACTTTCATTTAAGGATGATCTACAGTCTCATCCGTCAACCATCAAGGCAAACTGTCTGCCATGATAACAGGGTTCCAGCTGATGATGCTCCCTTAAGGTTATTTAGAGCTGTTTTGAAAGGAGTTGAACAGGAGTTCAAGAGGTCGTCCATCGATTATGATGATATTATGTTGTGTTTGAATGCAGTATTGAAGTTCTTAAGAAATATGTGTGAAGAGCTAAGTCTGGATGGTAGTGACAGAAATGGCTTGCATCATATATCACTTCAGTATATGGAGGCTGTCAGTGAGGTGATAGAACCTACTCTAATGGGATCCCCTCTTTACAAGGTGGCATTGAACATCAAGTATATTGAAAACCTGCAAATGATTCCTGACGTTGGATATGAAAAATCAGGTGTCTGCTCCATTGGTTATATGGAAATGGTTTCGCCAATGGTTTATTTAAGCGTACTTTATTTCACTGTGGTGGTTCGGTCAACTTTGAGTACGTCCAAAGCCGACTACATTATGCAGGGAATGCACCAATATTTTAAATTTATGTTATCATCTTTTGGTCCCCTGGAAAGTCTCGTTATCACAACTGAATTATTGTACAAGCACACAGGGCCAGGCTGCTTAACGATGTGGATAGCAATAGCAGAATGTCTGAAAAACTACATAAATGATGTGAAGGATCTTTCATTGTTCAAAATGGACTCTGAGAGTAAGGGTTGTTATGCCATGAGCCATCTAATGTCCTATCCACTGCTTGTATGCTCCTGCGCCCAGAAAGACATTATGTCAGCAATGAGTGGATGTCCCTCAGAAACCCCTTCTTCTCCACAGATGAATGCTGTGCTTGAACAAGTTATCACATTATGGAAGTCCCTCTATGGTTCTATCTGTACCTCACTGTCTGGGTGTATCATTGTTGGCAGTGTCTCTGAGGCTCTAGTTTCTGTACTGGACAGGTGCCTTGACAAGTATACTAGCATGCTTACATGTGCTAACGAGCTTCACTTAAACATCAAGGATTTGGATCTTCATCTCATTTCCTTATATGGTGATGCTTTTATGTGTATTCTGAAAAATTCCAGTTCTTCAGAATCAAGTTCAGATGGAAATACCAAGCATTGCTATGATTACAAGATCACTAGTGGCATTAAAGGCTGCTTGACTTTGGCCATTAGATATATGAAGTTGTCGCAGACAAAGATAGGAATAGACGCACCAATTGGTCTTCCTATGTTCTCTAGTTGCAGGTTATGTTCTGCATTGGCACTCTTCATCAGGTCCCTTCACTTGAAGCAAGATATTGTTTCCTTCTTTGAGATGATATCATGTCCACTGCTTGAGTGGCTGGGATACATGGAAATGCAAGATGAGGGCACCAGTGATCAATTTGAACTCCTATGGACCGAAATGCTTAATTGTTTAAGACGAAGTCAGCCTCCAATAGTCTTTGATTCTGCATTTCTCAAACTCCAGTCCTCTATCCTTGAGAAAACTCTTGATCACTCAAAACTGTCCATTTCAGAGCCAACCATCACCTTTTGGAACTCCATATATGGCGAACAGATCAACTTGGATTACCCTCAGACCTTGCTCCATGTCCTGGACAAGCTCTGGAGACAGGGAAGAATAAACCTCCACAAGAGAAGCCTACCTCTTCAAAGATGTCAATCTAAATCGGAAGCCATCACTGCTCCACCGAGATTCACGGTCAATACAGCACACAATAGAGGCTCTAAAAGAGTAGAACTAGTAGAGAATGCCACAGACAGTAAGCAGAAAGAAATGCCACATCCGAGTTTGAAAAGGAAGAGGTCGGAACTCACTGAGCATCAGAAGGAAGTGAGACGAGCACAACGAGGAAGGGGAAGGGATTGTGGCGGACACGGCATTGGTATCCAGACTTACACAACACTCAACTTTTCACAAGGGAATGAGGACTCACAGGGGAATGAGGATTCGCAAGACAGCGAAGATATCCGGAATCCAGAATGCATTTTGCAGATGTTGAGGAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04550 map04550
Pfam Domains
Protein Families
Rif1_N

Protein Analysis

1143

Amino Acids

128.74

Weight (kDa)

6.38

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rif1_N PF12231 19 - 301 7.5e-33 Rap1-interacting factor 1 N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1785, 2649
AccB1I GGYRCC 3 cut(s) 541, 2370, 2786
AccB7I CCANNNNNTGG 1 cut(s) 3013
AccI GTMKAC 2 cut(s) 1170, 2384
AccII CGCG 3 cut(s) 137, 417, 419
AccIII TCCGGA 1 cut(s) 3390
AciI CCGC 5 cut(s) 203, 246, 419, 760, 3291
AclWI GGATC 9 cut(s) 173, 615, 1018, 1196, 1239, 1725, 1738, 2100, 2445
AcoI YGGCCR 1 cut(s) 2580
AcsI RAATTY 5 cut(s) 261, 816, 1271, 1952, 2488
AcuI CTGAAG 2 cut(s) 1667, 2484
AcvI CACGTG 1 cut(s) 1104
AdeI CACNNNGTG 1 cut(s) 1107
AfaI GTAC 7 cut(s) 660, 958, 1869, 1906, 2012, 2305, 2361
AfiI CCNNNNNNNGG 3 cut(s) 1049, 1796, 3013
AflII CTTAAG 2 cut(s) 1510, 1615
AflIII ACRYGT 2 cut(s) 1101, 2399
AhdI GACNNNNNGTC 1 cut(s) 2197
AhlI ACTAGT 2 cut(s) 2552, 3160
AjnI CCWGG 7 cut(s) 867, 875, 1161, 1302, 1977, 2026, 3012
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AleI CACNNNNGTG 1 cut(s) 1883
Alw21I GWGCWC 2 cut(s) 146, 3265
Alw26I GTCTC 7 cut(s) 576, 777, 1448, 1991, 2344, 3022, 3250
AlwI GGATC 9 cut(s) 173, 615, 1018, 1196, 1239, 1725, 1738, 2100, 2445
Ama87I CYCGRG 1 cut(s) 821
Aor13HI TCCGGA 1 cut(s) 3390
AoxI GGCC 2 cut(s) 2024, 2580
ApeKI GCWGC 4 cut(s) 938, 1187, 2031, 2568
ApoI RAATTY 5 cut(s) 261, 816, 1271, 1952, 2488
ArsI GACNNNNNNTTYG 4 cut(s) 314, 346, 1163, 1195
AseI ATTAAT 2 cut(s) 801, 1242
AspLEI GCGC 5 cut(s) 139, 208, 417, 419, 2183
AspS9I GGNCC 4 cut(s) 1973, 2024, 2691, 2814
AsuHPI GGTGA 6 cut(s) 42, 896, 1004, 1720, 2474, 2938
AsuNHI GCTAGC 1 cut(s) 493
AvaI CYCGRG 1 cut(s) 821
AvaII GGWCC 3 cut(s) 1973, 2691, 2814
BaeGI GKGCMC 2 cut(s) 544, 2789
BalI TGGCCA 1 cut(s) 2582
BamHI GGATCC 1 cut(s) 1730
BanI GGYRCC 3 cut(s) 541, 2370, 2786
BanII GRGCYC 1 cut(s) 146
BbrPI CACGTG 1 cut(s) 1104
BbsI GAAGAC 2 cut(s) 565, 2630
Bbv12I GWGCWC 2 cut(s) 146, 3265
BbvI GCAGC 4 cut(s) 925, 1199, 2018, 2555
BceAI ACGGC 1 cut(s) 3313
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 7 cut(s) 869, 877, 1163, 1304, 1979, 2028, 3014
BciVI GTATCC 3 cut(s) 1389, 2756, 3317
BclI TGATCA 4 cut(s) 1032, 1155, 2794, 2911
BcoDI GTCTC 7 cut(s) 576, 777, 1448, 1991, 2344, 3022, 3250
BcuI ACTAGT 2 cut(s) 2552, 3160
BfaI CTAG 7 cut(s) 494, 767, 2351, 2388, 2553, 2652, 3161
BfmI CTRYAG 1 cut(s) 1438
BfrI CTTAAG 2 cut(s) 1510, 1615
BfuAI ACCTGC 2 cut(s) 1785, 2649
BfuI GTATCC 3 cut(s) 1389, 2756, 3317
BglII AGATCT 1 cut(s) 646
BisI GCNGC 5 cut(s) 204, 939, 1188, 2032, 2569
BlpI GCTNAGC 2 cut(s) 140, 566
BlsI GCNGC 5 cut(s) 205, 940, 1189, 2033, 2570
Bme1390I CCNGG 7 cut(s) 869, 877, 1163, 1304, 1979, 2028, 3014
Bme18I GGWCC 3 cut(s) 1973, 2691, 2814
BmeRI GACNNNNNGTC 1 cut(s) 2197
BmeT110I CYCGRG 1 cut(s) 821
BmgT120I GGNCC 4 cut(s) 1973, 2024, 2691, 2814
BmiI GGNNCC 8 cut(s) 393, 543, 1491, 1732, 1975, 2372, 2693, 2788
BmrFI CCNGG 7 cut(s) 869, 877, 1163, 1304, 1979, 2028, 3014
BmsI GCATC 8 cut(s) 106, 687, 883, 1062, 1492, 1678, 2455, 3250
BmtI GCTAGC 1 cut(s) 497
BpiI GAAGAC 2 cut(s) 565, 2630
BpmI CTGGAG 2 cut(s) 2867, 3046
Bpu1102I GCTNAGC 2 cut(s) 140, 566
BpuEI CTTGAG 3 cut(s) 1266, 2771, 2918
Bsa29I ATCGAT 1 cut(s) 1569
BsaAI YACGTR 2 cut(s) 1104, 1298
BsaBI GATNNNNATC 4 cut(s) 1052, 2436, 2442, 3079
BsaI GGTCTC 1 cut(s) 777
BsaJI CCNNGG 5 cut(s) 654, 867, 876, 1397, 1977
BsaWI WCCGGW 1 cut(s) 3390
Bsc4I CCNNNNNNNGG 3 cut(s) 1049, 1796, 3013
Bse1I ACTGG 5 cut(s) 1012, 2367, 2493, 2790, 2884
Bse3DI GCAATG 2 cut(s) 2211, 2530
Bse8I GATNNNNATC 4 cut(s) 1052, 2436, 2442, 3079
BseAI TCCGGA 1 cut(s) 3390
BseBI CCWGG 7 cut(s) 869, 877, 1163, 1304, 1979, 2028, 3014
BseCI ATCGAT 1 cut(s) 1569
BseDI CCNNGG 5 cut(s) 654, 867, 876, 1397, 1977
BseJI GATNNNNATC 4 cut(s) 1052, 2436, 2442, 3079
BseLI CCNNNNNNNGG 3 cut(s) 1049, 1796, 3013
BseMI GCAATG 2 cut(s) 2211, 2530
BseMII CTCAG 9 cut(s) 27, 131, 161, 365, 2109, 2235, 2334, 3008, 3228
BseNI ACTGG 5 cut(s) 1012, 2367, 2493, 2790, 2884
BsePI GCGCGC 1 cut(s) 415
BseSI GKGCMC 2 cut(s) 544, 2789
BseXI GCAGC 4 cut(s) 925, 1199, 2018, 2555
BseYI CCCAGC 1 cut(s) 2758
Bsh1236I CGCG 3 cut(s) 137, 417, 419
BshFI GGCC 2 cut(s) 2026, 2582
BshNI GGYRCC 3 cut(s) 541, 2370, 2786
BshVI ATCGAT 1 cut(s) 1569
BsiHKAI GWGCWC 2 cut(s) 146, 3265
BsiHKCI CYCGRG 1 cut(s) 821
BsiSI CCGG 1 cut(s) 3391
BslFI GGGAC 4 cut(s) 1959, 2202, 2269, 2677
BslI CCNNNNNNNGG 3 cut(s) 1049, 1796, 3013
BsmAI GTCTC 7 cut(s) 576, 777, 1448, 1991, 2344, 3022, 3250
BsmBI CGTCTC 1 cut(s) 3250
BsmFI GGGAC 4 cut(s) 1959, 2202, 2269, 2677
BsmI GAATGC 6 cut(s) 1345, 1603, 1941, 2254, 3175, 3407
BsnI GGCC 2 cut(s) 2026, 2582
Bso31I GGTCTC 1 cut(s) 777
BsoBI CYCGRG 1 cut(s) 821
Bsp1286I GDGCHC 4 cut(s) 146, 544, 2789, 3265
Bsp13I TCCGGA 1 cut(s) 3390
Bsp1407I TGTACA 1 cut(s) 2010
Bsp1720I GCTNAGC 2 cut(s) 140, 566
Bsp19I CCATGG 1 cut(s) 1397
BspACI CCGC 5 cut(s) 203, 246, 419, 760, 3291
BspANI GGCC 2 cut(s) 2026, 2582
BspCNI CTCAG 9 cut(s) 26, 132, 160, 366, 2110, 2234, 2335, 3007, 3229
BspDI ATCGAT 1 cut(s) 1569
BspEI TCCGGA 1 cut(s) 3390
BspFNI CGCG 3 cut(s) 137, 417, 419
BspHI TCATGA 1 cut(s) 1035
BspLI GGNNCC 8 cut(s) 393, 543, 1491, 1732, 1975, 2372, 2693, 2788
BspMI ACCTGC 2 cut(s) 1785, 2649
BspOI GCTAGC 1 cut(s) 497
BspPI GGATC 9 cut(s) 173, 615, 1018, 1196, 1239, 1725, 1738, 2100, 2445
BspQI GCTCTTC 1 cut(s) 1626
BspT107I GGYRCC 3 cut(s) 541, 2370, 2786
BspTI CTTAAG 2 cut(s) 1510, 1615
BspTNI GGTCTC 1 cut(s) 777
BsrDI GCAATG 2 cut(s) 2211, 2530
BsrGI TGTACA 1 cut(s) 2010
BsrI ACTGG 5 cut(s) 1012, 2367, 2493, 2790, 2884
BssECI CCNNGG 5 cut(s) 654, 867, 876, 1397, 1977
BssHII GCGCGC 1 cut(s) 415
BssNAI GTATAC 1 cut(s) 2385
BssT1I CCWWGG 2 cut(s) 654, 1397
Bst1107I GTATAC 1 cut(s) 2385
Bst2UI CCWGG 7 cut(s) 869, 877, 1163, 1304, 1979, 2028, 3014
Bst6I CTCTTC 4 cut(s) 1626, 2687, 3072, 3215
BstAFI CTTAAG 2 cut(s) 1510, 1615
BstAPI GCANNNNNTGC 3 cut(s) 706, 935, 1936
BstAUI TGTACA 1 cut(s) 2010
BstBAI YACGTR 2 cut(s) 1104, 1298
BstC8I GCNNGC 7 cut(s) 210, 417, 495, 1093, 1313, 1667, 2393
BstDSI CCRYGG 1 cut(s) 1397
BstFNI CGCG 3 cut(s) 137, 417, 419
BstHHI GCGC 5 cut(s) 139, 208, 417, 419, 2183
BstMAI GTCTC 7 cut(s) 576, 777, 1448, 1991, 2344, 3022, 3250
BstNI CCWGG 7 cut(s) 869, 877, 1163, 1304, 1979, 2028, 3014
BstNSI RCATGY 2 cut(s) 2395, 2403
BstSCI CCNGG 7 cut(s) 867, 875, 1161, 1302, 1977, 2026, 3012
BstSFI CTRYAG 1 cut(s) 1438
BstSLI GKGCMC 2 cut(s) 544, 2789
BstUI CGCG 3 cut(s) 137, 417, 419
BstV1I GCAGC 4 cut(s) 925, 1199, 2018, 2555
BstV2I GAAGAC 2 cut(s) 565, 2630
BstX2I RGATCY 7 cut(s) 620, 646, 1010, 1231, 1730, 2092, 2437
BstXI CCANNNNNNTGG 1 cut(s) 1007
BstYI RGATCY 7 cut(s) 620, 646, 1010, 1231, 1730, 2092, 2437
BstZ17I GTATAC 1 cut(s) 2385
Bsu15I ATCGAT 1 cut(s) 1569
BsuI GTATCC 3 cut(s) 1389, 2756, 3317
BsuRI GGCC 2 cut(s) 2026, 2582
BsuTUI ATCGAT 1 cut(s) 1569
BtgI CCRYGG 1 cut(s) 1397
BtsI GCAGTG 4 cut(s) 2162, 2341, 2744, 3099
BveI ACCTGC 2 cut(s) 1785, 2649
Cac8I GCNNGC 7 cut(s) 210, 417, 495, 1093, 1313, 1667, 2393
CciI TCATGA 1 cut(s) 1035
CfoI GCGC 5 cut(s) 139, 208, 417, 419, 2183
Cfr13I GGNCC 4 cut(s) 1973, 2024, 2691, 2814
ClaI ATCGAT 1 cut(s) 1569
CseI GACGC 1 cut(s) 2633
CsiI ACCWGGT 2 cut(s) 1161, 1302
Csp6I GTAC 7 cut(s) 659, 957, 1868, 1905, 2011, 2304, 2360
CspCI CAANNNNNGTGG 2 cut(s) 1073, 1108
CviQI GTAC 7 cut(s) 659, 957, 1868, 1905, 2011, 2304, 2360
DraI TTTAAA 3 cut(s) 675, 1276, 1951
DraIII CACNNNGTG 1 cut(s) 1107
DriI GACNNNNNGTC 1 cut(s) 2197
EaeI YGGCCR 1 cut(s) 2580
Eam1104I CTCTTC 4 cut(s) 1626, 2687, 3072, 3215
Eam1105I GACNNNNNGTC 1 cut(s) 2197
EarI CTCTTC 4 cut(s) 1626, 2687, 3072, 3215
EciI GGCGGA 1 cut(s) 3306
Ecl136II GAGCTC 1 cut(s) 144
Eco130I CCWWGG 2 cut(s) 654, 1397
Eco24I GRGCYC 1 cut(s) 146
Eco31I GGTCTC 1 cut(s) 777
Eco32I GATATC 2 cut(s) 2736, 3388
Eco47I GGWCC 3 cut(s) 1973, 2691, 2814
Eco53kI GAGCTC 1 cut(s) 144
Eco57I CTGAAG 2 cut(s) 1667, 2484
Eco72I CACGTG 1 cut(s) 1104
Eco88I CYCGRG 1 cut(s) 821
EcoICRI GAGCTC 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 2691
EcoRII CCWGG 7 cut(s) 867, 875, 1161, 1302, 1977, 2026, 3012
EcoRV GATATC 2 cut(s) 2736, 3388
EcoT14I CCWWGG 2 cut(s) 654, 1397
EcoT22I ATGCAT 1 cut(s) 3407
EcoT38I GRGCYC 1 cut(s) 146
ErhI CCWWGG 2 cut(s) 654, 1397
Esp3I CGTCTC 1 cut(s) 3250
FalI AAGNNNNNCTT 2 cut(s) 668, 700
FaqI GGGAC 4 cut(s) 1959, 2202, 2269, 2677
FauI CCCGC 1 cut(s) 253
FbaI TGATCA 4 cut(s) 1032, 1155, 2794, 2911
FblI GTMKAC 2 cut(s) 1170, 2384
Fnu4HI GCNGC 5 cut(s) 204, 939, 1188, 2032, 2569
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 5 cut(s) 204, 939, 1188, 2032, 2569
FspBI CTAG 7 cut(s) 494, 767, 2351, 2388, 2553, 2652, 3161
GlaI GCGC 5 cut(s) 138, 207, 416, 418, 2182
GluI GCNGC 5 cut(s) 204, 939, 1188, 2032, 2569
GsaI CCCAGC 1 cut(s) 2762
GsuI CTGGAG 2 cut(s) 2867, 3046
HaeIII GGCC 2 cut(s) 2026, 2582
HapII CCGG 1 cut(s) 3391
HgaI GACGC 1 cut(s) 2633
HhaI GCGC 5 cut(s) 139, 208, 417, 419, 2183
Hin6I GCGC 5 cut(s) 137, 206, 415, 417, 2181
HinP1I GCGC 5 cut(s) 137, 206, 415, 417, 2181
HincII GTYRAC 3 cut(s) 459, 1454, 1896
HindII GTYRAC 3 cut(s) 459, 1454, 1896
HpaII CCGG 1 cut(s) 3391
HphI GGTGA 6 cut(s) 42, 896, 1004, 1720, 2474, 2938
Hpy166II GTNNAC 8 cut(s) 380, 459, 1084, 1171, 1454, 1896, 2385, 2744
Hpy8I GTNNAC 8 cut(s) 380, 459, 1084, 1171, 1454, 1896, 2385, 2744
Hpy99I CGWCG 5 cut(s) 165, 173, 188, 191, 194
HpyCH4IV ACGT 4 cut(s) 1103, 1297, 1794, 1907
HpySE526I ACGT 4 cut(s) 1103, 1297, 1794, 1907
HspAI GCGC 5 cut(s) 137, 206, 415, 417, 2181
Kpn2I TCCGGA 1 cut(s) 3390
Ksp22I TGATCA 4 cut(s) 1032, 1155, 2794, 2911
LguI GCTCTTC 1 cut(s) 1626
LmnI GCTCC 7 cut(s) 397, 1348, 1510, 1826, 2180, 3009, 3109
Lsp1109I GCAGC 4 cut(s) 925, 1199, 2018, 2555
LweI GCATC 8 cut(s) 106, 687, 883, 1062, 1492, 1678, 2455, 3250
MabI ACCWGGT 2 cut(s) 1161, 1302
MaeI CTAG 7 cut(s) 494, 767, 2351, 2388, 2553, 2652, 3161
MaeII ACGT 4 cut(s) 1103, 1297, 1794, 1907
MaeIII GTNAC 4 cut(s) 69, 992, 1298, 1652
MfeI CAATTG 1 cut(s) 2631
MflI RGATCY 7 cut(s) 620, 646, 1010, 1231, 1730, 2092, 2437
MhlI GDGCHC 4 cut(s) 146, 544, 2789, 3265
MlsI TGGCCA 1 cut(s) 2582
MluNI TGGCCA 1 cut(s) 2582
MlyI GAGTC 4 cut(s) 79, 839, 2108, 3344
MmeI TCCRAC 5 cut(s) 124, 143, 1395, 1777, 3207
Mox20I TGGCCA 1 cut(s) 2582
Mph1103I ATGCAT 1 cut(s) 3407
MroI TCCGGA 1 cut(s) 3390
MscI TGGCCA 1 cut(s) 2582
MslI CAYNNNNRTG 6 cut(s) 217, 695, 748, 1883, 2535, 2771
Msp20I TGGCCA 1 cut(s) 2582
MspA1I CMGCKG 2 cut(s) 373, 1496
MspCI CTTAAG 2 cut(s) 1510, 1615
MspI CCGG 1 cut(s) 3391
MspR9I CCNGG 7 cut(s) 869, 877, 1163, 1304, 1979, 2028, 3014
MunI CAATTG 1 cut(s) 2631
Mva1269I GAATGC 6 cut(s) 1345, 1603, 1941, 2254, 3175, 3407
MvaI CCWGG 7 cut(s) 869, 877, 1163, 1304, 1979, 2028, 3014
MvnI CGCG 3 cut(s) 137, 417, 419
NcoI CCATGG 1 cut(s) 1397
NheI GCTAGC 1 cut(s) 493
NlaIV GGNNCC 8 cut(s) 393, 543, 1491, 1732, 1975, 2372, 2693, 2788
NmuCI GTSAC 3 cut(s) 69, 992, 1652
NsiI ATGCAT 1 cut(s) 3407
NspI RCATGY 2 cut(s) 2395, 2403
OliI CACNNNNGTG 1 cut(s) 1883
PacI TTAATTAA 1 cut(s) 801
PaeI GCATGC 1 cut(s) 2395
PagI TCATGA 1 cut(s) 1035
PauI GCGCGC 1 cut(s) 415
PciI ACATGT 1 cut(s) 2399
PciSI GCTCTTC 1 cut(s) 1626
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 3265
PctI GAATGC 6 cut(s) 1345, 1603, 1941, 2254, 3175, 3407
PflFI GACNNNGTC 1 cut(s) 2841
PflMI CCANNNNNTGG 1 cut(s) 3013
PfoI TCCNGGA 1 cut(s) 3012
PkrI GCNGC 5 cut(s) 205, 940, 1189, 2033, 2570
PleI GAGTC 4 cut(s) 79, 839, 2108, 3344
PmaCI CACGTG 1 cut(s) 1104
PmlI CACGTG 1 cut(s) 1104
PpsI GAGTC 4 cut(s) 79, 839, 2108, 3344
Ppu21I YACGTR 2 cut(s) 1104, 1298
PpuMI RGGWCCY 1 cut(s) 2691
PscI ACATGT 1 cut(s) 2399
PshBI ATTAAT 2 cut(s) 801, 1242
Psp124BI GAGCTC 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 2691
Psp6I CCWGG 7 cut(s) 867, 875, 1161, 1302, 1977, 2026, 3012
PspCI CACGTG 1 cut(s) 1104
PspFI CCCAGC 1 cut(s) 2758
PspGI CCWGG 7 cut(s) 867, 875, 1161, 1302, 1977, 2026, 3012
PspN4I GGNNCC 8 cut(s) 393, 543, 1491, 1732, 1975, 2372, 2693, 2788
PspPI GGNCC 4 cut(s) 1973, 2024, 2691, 2814
PspPPI RGGWCCY 1 cut(s) 2691
PsuI RGATCY 7 cut(s) 620, 646, 1010, 1231, 1730, 2092, 2437
PsyI GACNNNGTC 1 cut(s) 2841
PteI GCGCGC 1 cut(s) 415
PvuII CAGCTG 2 cut(s) 373, 1496
RsaI GTAC 7 cut(s) 660, 958, 1869, 1906, 2012, 2305, 2361
RsaNI GTAC 7 cut(s) 659, 957, 1868, 1905, 2011, 2304, 2360
RseI CAYNNNNRTG 6 cut(s) 217, 695, 748, 1883, 2535, 2771
SacI GAGCTC 1 cut(s) 146
SapI GCTCTTC 1 cut(s) 1626
SatI GCNGC 5 cut(s) 204, 939, 1188, 2032, 2569
Sau96I GGNCC 4 cut(s) 1973, 2024, 2691, 2814
SchI GAGTC 4 cut(s) 79, 839, 2108, 3344
ScrFI CCNGG 7 cut(s) 869, 877, 1163, 1304, 1979, 2028, 3014
SduI GDGCHC 4 cut(s) 146, 544, 2789, 3265
SexAI ACCWGGT 2 cut(s) 1161, 1302
SfaNI GCATC 8 cut(s) 106, 687, 883, 1062, 1492, 1678, 2455, 3250
SfcI CTRYAG 1 cut(s) 1438
SinI GGWCC 3 cut(s) 1973, 2691, 2814
SmiMI CAYNNNNRTG 6 cut(s) 217, 695, 748, 1883, 2535, 2771
SmlI CTYRAG 5 cut(s) 1281, 1510, 1615, 2750, 2897
SmoI CTYRAG 5 cut(s) 1281, 1510, 1615, 2750, 2897
SpeI ACTAGT 2 cut(s) 2552, 3160
SphI GCATGC 1 cut(s) 2395
SsiI CCGC 5 cut(s) 203, 246, 419, 760, 3291
SspI AATATT 1 cut(s) 1946
SspMI CTAG 7 cut(s) 494, 767, 2351, 2388, 2553, 2652, 3161
SstI GAGCTC 1 cut(s) 146
StyD4I CCNGG 7 cut(s) 867, 875, 1161, 1302, 1977, 2026, 3012
StyI CCWWGG 2 cut(s) 654, 1397
TaiI ACGT 4 cut(s) 1106, 1300, 1797, 1910
TaqI TCGA 4 cut(s) 5, 24, 83, 1569
TaqII GACCGA 2 cut(s) 1881, 2831
TatI WGTACW 2 cut(s) 2010, 2359
TauI GCSGC 1 cut(s) 206
TseFI GTSAC 3 cut(s) 69, 992, 1652
TseI GCWGC 4 cut(s) 938, 1187, 2031, 2568
Tsp45I GTSAC 3 cut(s) 69, 992, 1652
TspGWI ACGGA 2 cut(s) 149, 1439
Tth111I GACNNNGTC 1 cut(s) 2841
Van91I CCANNNNNTGG 1 cut(s) 3013
Vha464I CTTAAG 2 cut(s) 1510, 1615
VpaK11BI GGWCC 3 cut(s) 1973, 2691, 2814
VspI ATTAAT 2 cut(s) 801, 1242
XapI RAATTY 5 cut(s) 261, 816, 1271, 1952, 2488
XceI RCATGY 2 cut(s) 2395, 2403
XcmI CCANNNNNNNNNTGG 2 cut(s) 1831, 2751
XmiI GTMKAC 2 cut(s) 1170, 2384
XspI CTAG 7 cut(s) 494, 767, 2351, 2388, 2553, 2652, 3161
Zsp2I ATGCAT 1 cut(s) 3407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.