RchiOBHm_Chr2g0087621

Rap1-interacting factor 1 N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006378
Physical Location & Seq
Reverse (-)
2316983 .. 2323131
6149 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46306

Sequence Viewer

Length: 3414 bp
ATGTCGAATCTCTCAGACCAGCTCCAACAAATCAAATCCCTAATTTCTTCACCCACCAAATCAAACAAGTCACTCGCTTACTCCACTCTGTCACACCTTCAACAACAGTCCTCCGATGCTCTGATTCAAACACTCGCGCTGAGCTCGCAGAGCCTTCTCCGTTGGATCGTCGACGATATTCACGACGACGATGAAGAAATTGCGGCGCAGGCATTGAAATGTTTGGGTTTTATGATATACCATCCGGCCATTGTTTCCGAAATTTCAGTGGAGGATGTGAAGTTGGTTTTGGAATCATTGGTTAAACTCATTACAACTACGAAAATTAAGTCGGTTTGTAATTTAGGAGTGTGGTGCATATCTATACAACAGCTGAGTGAACCACTTTTGGCTCCCCATTTCCATTCTCTTTTGCGCGCAGTAGTTCATGCCATTGACAATCCCATTGGTTCTCTGTCAACAACTTTTGAGGCTATTCAGGCTGTCATTAAGCTAGCTACTCTTTTAAGTGAGAGCATGAGAGAGTTGTCTCATATATGGGCACCTCCAATATATAGAAGACTGCTTAGCTCTGATAAGAGAGACAGGGATATGTCAGAAAGGTGTCTGTTGAAGATTAGATCCACAATCATACCCCCTCCAATAGATCTCTCCAAGGTACTTGTCAGAGATTTAAAGACAAAGTTGCTCACTATGATGCACGATATGCTAACCAGTGGTATGAAGGTTCAAACTATTCAAGCATGGGGATGGTATATCCGCTTACTAGGGTCTCATGCCTTGAAGAACAGACATTTAATTAATGATATGCTGAAAATTCCCGAGAAAACATTTTCAGATCACGACTCACAAGTTCAGATTGCTTCCCAGGTTGCCTGGGAAGGTCTTATTGATGCTCTTATTCACCCTCCAATGATATTACCGTGTGCAACAAATGCTGCAAAAGAGGATGATGGTACGCAAAAAAAGGGAACACATAAAGGAAACAGTGGTGACACCCAAAACACTGGATCTTTGAAAAGCGTAAGGCTGATCATGACACCACTGATAGGCATCATGTTGAGTAACTGTGATGTATCTGTCCACATGGCTTGCTTGAACACATGGTGCTATCTATTACATAGACTTGATACCTCTCTGAATGATTCTTCAATGATCAACCTGGTAGTAGACCCTATCTTTGAAGCAGCTTTTCAACTTGATCCTGATGGTAAGAGCTTCTGGGCTAGGGATCTGTGCATTAATTTGCTTGATGATTTTATATCAGCAAAATGTAAACCTCAAGGCTATGAACCACGTAACCAGGTAAGCCAGCAGTTATCAGCCAAAACCTCTATGAATGCTCCCATTATATCTGGCAGTTGCTCATGGAAGCAGTATCCTATTAAATGGTTGCCATGGGATTTAAGTCTGTTGGACTTTCATTTAAAGATGATCTACAGTCTCATCCGTCAACCATCAAGGCAAACTGTCTGCCATGATAACAGGGATGCTCCCTTAAGGTTATTTAGAGCTGTTTTGAAAGGAGTTGAACAGGAGTTCAAGAGGTCGTCCATCGATTATGATGATATTATGTTGTGTTTGAATGCAGTATTGAAGTTCTTAAGAAATATGTGTGAAGAACTAAGTCTGGATGGTAGTGACAGAAATGGCTTGCATCATCTATCACTTCAGTATATGGAGGCTGTCAGCGAGGTGATAGAACCTACTCTAATGGGATCCCCTCTTTACAGGGTGGCATTGAACATCAAGTATATTGAAAACCTGCAAATGATTCCTGACGTTGGATATGAAAAATCAGGTGTCTGCTCTATTGGTTATATGGAACTTGTTTCGCCAATGGTTTATTTAAGCGTACTTTATTTCACTGTGGTGGTTCGGTCAACTTTTAGTACGTCCAAAGCTGACTACATTCTGCAGGGAATGCAGCAATATTTTAAATTTATGTTATCATCTTTTGGTCCCCTGGAAAGTCTCGTTATCACAACTGAATTATTGTACAAGCACACAGGGCCAGGCTGCTTAACGATGTGGATAGCAATAGCAGAAAGTCTGAAAAACTACATAAATGATGTGAAGGATCTTTCATTGTTCAAAATGGACTCTGAGAGTAAGGGTTGTTATGCCATGAGCCATCTAATGTCCTATCCACTGCTTGTATGCTCCTGCGCCCAGAAAGACATTATGTCAGCAATGAGTGGATGTCCCTTAGAAACCCCTTCTTCTCCATGGATGAATACTGAGCTTGAACAAGTTATCACATTATGGAAGTCCCTCTATGGTTCTATCTGTACCTCACTGTCTGGGTGTATCATTGTTGGCAGTTTCTCTGAGGCTCTAGTTTCTGTATTGGACAGGTGCCTTGACAAGTATACTAGCATGCTTACATGTACTAACGAGCTTCACTTAAACTTCAAGGATTTGGATCTTCATCTCATTTCCTTATATGGTGATGCTTTTATGTGTATTCTGAAAAATTCCAGTTCTTCAGAATCAAGTTCCGATGGAAATACCAAGCATTACTATGATTACAAGATCACTAGTGGCATTAAAGGCTGCTTGACTTTGGCCATTAGATATATGAAGCTGTTGCAGACAAAGATAGGAACAGACGCACCAATTGGTCTTCCTATGTTCTCTAGGTTATGTTCTGCATTGGCACTCTTCATCAGGTCCCTTCACTTGAAGCAAGATATTGTTTCCTTCTTTGAGATGATATCATGTCCACTGCTTGAGTGGCTGGGATACATGGAAATGCAAGATGAGGGCACCAGTGATCAATTTGAACTCCTATGGACCGAAATGCTTAATTGTTTAAGACGAAGTCAGCCTCCAATAGTCTTTGATTCTGCATTTCTCAAACTCCAGGCCTCTATCCTTGAGAAAACTCTTGATCACTCAAAACTGTCCATTTCAGAGCCAACCATCACCTTTTGGAACTCCATATATGGCGAACAGATCAACTTGGATTACCCTCAGACCTTGCTCCATGTCCTGGACAAGCTCTGGAGACAGGGAAGAATAAACCTCCACAAGAGAAGCCTACCTCTTCAAAGATGTCAATCTAAATCGGAAGCCATCACTGCTCCACCGAGATTCACGGTCAATACAGCACACAATAGAGGCTCTAAAAGAGTAGAACTAGTAGAGAATACCAGAGACACTAAGCAGAAGGAAATGCCACATTTGAGTTTGAAAAGGAAGAGGTCGGAACTCACTGAGCATCAGAAGGAAGTGAGACGAGCACAACGAGGAAGGGGAAGGGATTGTGGCGGACACGGCATTGGTATCCAGACTTACACAACACTCGACTTTTCACAAGGGAATGAGGACTCACAGGGGAATGAGGATTTGCAAGACAGCGAAGATATCCGGAATCCAGAATGCATTTTGCAGATGTTGAGGAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04550 map04550
Pfam Domains
Protein Families
Rif1_N

Protein Analysis

1137

Amino Acids

128.48

Weight (kDa)

6.49

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rif1_N PF12231 19 - 301 2.1e-32 Rap1-interacting factor 1 N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1773
AccB1I GGYRCC 3 cut(s) 541, 2358, 2768
AccB7I CCANNNNNTGG 1 cut(s) 2995
AccI GTMKAC 3 cut(s) 171, 1170, 2372
AccII CGCG 2 cut(s) 137, 417
AccIII TCCGGA 1 cut(s) 3372
AciI CCGC 3 cut(s) 203, 760, 3273
AclWI GGATC 9 cut(s) 173, 615, 1018, 1196, 1239, 1713, 1726, 2088, 2433
AcoI YGGCCR 2 cut(s) 246, 2568
AcsI RAATTY 4 cut(s) 261, 816, 1940, 2476
AcuI CTGAAG 2 cut(s) 1655, 2472
AdeI CACNNNGTG 1 cut(s) 1107
AfaI GTAC 7 cut(s) 660, 958, 1857, 1894, 2000, 2293, 2392
AfiI CCNNNNNNNGG 3 cut(s) 1049, 1784, 2995
AflII CTTAAG 2 cut(s) 1498, 1603
AflIII ACRYGT 1 cut(s) 2387
AhdI GACNNNNNGTC 1 cut(s) 2185
AhlI ACTAGT 2 cut(s) 2540, 3142
AjnI CCWGG 8 cut(s) 867, 875, 1161, 1302, 1965, 2014, 2865, 2994
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AleI CACNNNNGTG 1 cut(s) 1871
Alw21I GWGCWC 2 cut(s) 146, 3247
Alw26I GTCTC 8 cut(s) 534, 576, 777, 1448, 1979, 3004, 3153, 3232
AlwI GGATC 9 cut(s) 173, 615, 1018, 1196, 1239, 1713, 1726, 2088, 2433
Ama87I CYCGRG 1 cut(s) 821
Aor13HI TCCGGA 1 cut(s) 3372
AoxI GGCC 4 cut(s) 246, 2012, 2568, 2868
ApeKI GCWGC 5 cut(s) 938, 1187, 1927, 2019, 2556
ApoI RAATTY 4 cut(s) 261, 816, 1940, 2476
ArsI GACNNNNNNTTYG 4 cut(s) 314, 346, 1163, 1195
AseI ATTAAT 2 cut(s) 801, 1242
AspLEI GCGC 5 cut(s) 139, 208, 417, 419, 2171
AspS9I GGNCC 4 cut(s) 1961, 2012, 2673, 2796
AsuHPI GGTGA 6 cut(s) 42, 896, 1004, 1708, 2462, 2920
AsuNHI GCTAGC 1 cut(s) 493
AvaI CYCGRG 1 cut(s) 821
AvaII GGWCC 3 cut(s) 1961, 2673, 2796
BaeGI GKGCMC 2 cut(s) 544, 2771
BalI TGGCCA 1 cut(s) 2570
BamHI GGATCC 1 cut(s) 1718
BanI GGYRCC 3 cut(s) 541, 2358, 2768
BanII GRGCYC 1 cut(s) 146
BbsI GAAGAC 2 cut(s) 565, 2618
Bbv12I GWGCWC 2 cut(s) 146, 3247
BbvI GCAGC 5 cut(s) 925, 1199, 1939, 2006, 2543
BceAI ACGGC 1 cut(s) 3295
BciT130I CCWGG 8 cut(s) 869, 877, 1163, 1304, 1967, 2016, 2867, 2996
BciVI GTATCC 3 cut(s) 1389, 2738, 3299
BclI TGATCA 4 cut(s) 1032, 1155, 2776, 2893
BcoDI GTCTC 8 cut(s) 534, 576, 777, 1448, 1979, 3004, 3153, 3232
BcuI ACTAGT 2 cut(s) 2540, 3142
BfaI CTAG 8 cut(s) 494, 767, 1227, 2339, 2376, 2541, 2640, 3143
BfmI CTRYAG 2 cut(s) 1438, 1916
BfrI CTTAAG 2 cut(s) 1498, 1603
BfuAI ACCTGC 1 cut(s) 1773
BfuI GTATCC 3 cut(s) 1389, 2738, 3299
BglII AGATCT 1 cut(s) 646
BisI GCNGC 6 cut(s) 204, 939, 1188, 1928, 2020, 2557
BlpI GCTNAGC 2 cut(s) 140, 566
BlsI GCNGC 6 cut(s) 205, 940, 1189, 1929, 2021, 2558
Bme1390I CCNGG 8 cut(s) 869, 877, 1163, 1304, 1967, 2016, 2867, 2996
Bme18I GGWCC 3 cut(s) 1961, 2673, 2796
BmeRI GACNNNNNGTC 1 cut(s) 2185
BmeT110I CYCGRG 1 cut(s) 821
BmgT120I GGNCC 4 cut(s) 1961, 2012, 2673, 2796
BmiI GGNNCC 7 cut(s) 393, 543, 1720, 1963, 2360, 2675, 2770
BmrFI CCNGG 8 cut(s) 869, 877, 1163, 1304, 1967, 2016, 2867, 2996
BmsI GCATC 8 cut(s) 106, 687, 883, 1062, 1480, 1666, 2443, 3232
BmtI GCTAGC 1 cut(s) 497
BpiI GAAGAC 2 cut(s) 565, 2618
BpmI CTGGAG 2 cut(s) 2849, 3028
Bpu1102I GCTNAGC 2 cut(s) 140, 566
BpuEI CTTGAG 3 cut(s) 1266, 2753, 2900
Bsa29I ATCGAT 1 cut(s) 1557
BsaAI YACGTR 1 cut(s) 1298
BsaBI GATNNNNATC 4 cut(s) 1052, 2424, 2430, 3061
BsaI GGTCTC 1 cut(s) 777
BsaJI CCNNGG 6 cut(s) 654, 867, 876, 1397, 1965, 2228
BsaWI WCCGGW 1 cut(s) 3372
Bsc4I CCNNNNNNNGG 3 cut(s) 1049, 1784, 2995
Bse1I ACTGG 4 cut(s) 714, 1012, 2481, 2772
Bse3DI GCAATG 1 cut(s) 2199
Bse8I GATNNNNATC 4 cut(s) 1052, 2424, 2430, 3061
BseAI TCCGGA 1 cut(s) 3372
BseBI CCWGG 8 cut(s) 869, 877, 1163, 1304, 1967, 2016, 2867, 2996
BseCI ATCGAT 1 cut(s) 1557
BseDI CCNNGG 6 cut(s) 654, 867, 876, 1397, 1965, 2228
BseGI GGATG 9 cut(s) 241, 280, 755, 955, 1446, 1495, 1639, 2207, 2238
BseJI GATNNNNATC 4 cut(s) 1052, 2424, 2430, 3061
BseLI CCNNNNNNNGG 3 cut(s) 1049, 1784, 2995
BseMI GCAATG 1 cut(s) 2199
BseMII CTCAG 8 cut(s) 27, 131, 365, 2097, 2232, 2322, 2990, 3210
BseNI ACTGG 4 cut(s) 714, 1012, 2481, 2772
BsePI GCGCGC 1 cut(s) 415
BseSI GKGCMC 2 cut(s) 544, 2771
BseXI GCAGC 5 cut(s) 925, 1199, 1939, 2006, 2543
BseYI CCCAGC 1 cut(s) 2740
Bsh1236I CGCG 2 cut(s) 137, 417
BshFI GGCC 4 cut(s) 248, 2014, 2570, 2870
BshNI GGYRCC 3 cut(s) 541, 2358, 2768
BshVI ATCGAT 1 cut(s) 1557
BsiHKAI GWGCWC 2 cut(s) 146, 3247
BsiHKCI CYCGRG 1 cut(s) 821
BsiSI CCGG 2 cut(s) 245, 3373
BslFI GGGAC 4 cut(s) 1947, 2190, 2257, 2659
BslI CCNNNNNNNGG 3 cut(s) 1049, 1784, 2995
BsmAI GTCTC 8 cut(s) 534, 576, 777, 1448, 1979, 3004, 3153, 3232
BsmBI CGTCTC 1 cut(s) 3232
BsmFI GGGAC 4 cut(s) 1947, 2190, 2257, 2659
BsmI GAATGC 4 cut(s) 1345, 1591, 1929, 3389
BsnI GGCC 4 cut(s) 248, 2014, 2570, 2870
Bso31I GGTCTC 1 cut(s) 777
BsoBI CYCGRG 1 cut(s) 821
Bsp1286I GDGCHC 4 cut(s) 146, 544, 2771, 3247
Bsp13I TCCGGA 1 cut(s) 3372
Bsp1407I TGTACA 1 cut(s) 1998
Bsp1720I GCTNAGC 2 cut(s) 140, 566
Bsp19I CCATGG 2 cut(s) 1397, 2228
BspACI CCGC 3 cut(s) 203, 760, 3273
BspANI GGCC 4 cut(s) 248, 2014, 2570, 2870
BspCNI CTCAG 8 cut(s) 26, 132, 366, 2098, 2233, 2323, 2989, 3211
BspDI ATCGAT 1 cut(s) 1557
BspEI TCCGGA 1 cut(s) 3372
BspFNI CGCG 2 cut(s) 137, 417
BspHI TCATGA 1 cut(s) 1035
BspLI GGNNCC 7 cut(s) 393, 543, 1720, 1963, 2360, 2675, 2770
BspMAI CTGCAG 1 cut(s) 1920
BspMI ACCTGC 1 cut(s) 1773
BspOI GCTAGC 1 cut(s) 497
BspPI GGATC 9 cut(s) 173, 615, 1018, 1196, 1239, 1713, 1726, 2088, 2433
BspT107I GGYRCC 3 cut(s) 541, 2358, 2768
BspTI CTTAAG 2 cut(s) 1498, 1603
BspTNI GGTCTC 1 cut(s) 777
BsrDI GCAATG 1 cut(s) 2199
BsrGI TGTACA 1 cut(s) 1998
BsrI ACTGG 4 cut(s) 714, 1012, 2481, 2772
BssECI CCNNGG 6 cut(s) 654, 867, 876, 1397, 1965, 2228
BssHII GCGCGC 1 cut(s) 415
BssNAI GTATAC 1 cut(s) 2373
BssT1I CCWWGG 3 cut(s) 654, 1397, 2228
Bst1107I GTATAC 1 cut(s) 2373
Bst2UI CCWGG 8 cut(s) 869, 877, 1163, 1304, 1967, 2016, 2867, 2996
Bst6I CTCTTC 3 cut(s) 2669, 3054, 3197
BstAFI CTTAAG 2 cut(s) 1498, 1603
BstAPI GCANNNNNTGC 3 cut(s) 706, 935, 1924
BstAUI TGTACA 1 cut(s) 1998
BstBAI YACGTR 1 cut(s) 1298
BstC8I GCNNGC 8 cut(s) 146, 210, 417, 495, 1093, 1313, 1655, 2381
BstDSI CCRYGG 2 cut(s) 1397, 2228
BstF5I GGATG 9 cut(s) 241, 280, 755, 955, 1446, 1495, 1639, 2207, 2238
BstFNI CGCG 2 cut(s) 137, 417
BstHHI GCGC 5 cut(s) 139, 208, 417, 419, 2171
BstMAI GTCTC 8 cut(s) 534, 576, 777, 1448, 1979, 3004, 3153, 3232
BstNI CCWGG 8 cut(s) 869, 877, 1163, 1304, 1967, 2016, 2867, 2996
BstNSI RCATGY 2 cut(s) 2383, 2391
BstSCI CCNGG 8 cut(s) 867, 875, 1161, 1302, 1965, 2014, 2865, 2994
BstSFI CTRYAG 2 cut(s) 1438, 1916
BstSLI GKGCMC 2 cut(s) 544, 2771
BstUI CGCG 2 cut(s) 137, 417
BstV1I GCAGC 5 cut(s) 925, 1199, 1939, 2006, 2543
BstV2I GAAGAC 2 cut(s) 565, 2618
BstX2I RGATCY 7 cut(s) 620, 646, 1010, 1231, 1718, 2080, 2425
BstXI CCANNNNNNTGG 1 cut(s) 1007
BstYI RGATCY 7 cut(s) 620, 646, 1010, 1231, 1718, 2080, 2425
BstZ17I GTATAC 1 cut(s) 2373
Bsu15I ATCGAT 1 cut(s) 1557
BsuI GTATCC 3 cut(s) 1389, 2738, 3299
BsuRI GGCC 4 cut(s) 248, 2014, 2570, 2870
BsuTUI ATCGAT 1 cut(s) 1557
BtgI CCRYGG 2 cut(s) 1397, 2228
BtsCI GGATG 9 cut(s) 241, 280, 755, 955, 1446, 1495, 1639, 2207, 2238
BtsI GCAGTG 3 cut(s) 2150, 2726, 3081
BveI ACCTGC 1 cut(s) 1773
Cac8I GCNNGC 8 cut(s) 146, 210, 417, 495, 1093, 1313, 1655, 2381
CciI TCATGA 1 cut(s) 1035
CfoI GCGC 5 cut(s) 139, 208, 417, 419, 2171
Cfr13I GGNCC 4 cut(s) 1961, 2012, 2673, 2796
ClaI ATCGAT 1 cut(s) 1557
CseI GACGC 1 cut(s) 2621
CsiI ACCWGGT 2 cut(s) 1161, 1302
Csp6I GTAC 7 cut(s) 659, 957, 1856, 1893, 1999, 2292, 2391
CspCI CAANNNNNGTGG 2 cut(s) 1073, 1108
CviQI GTAC 7 cut(s) 659, 957, 1856, 1893, 1999, 2292, 2391
DraI TTTAAA 3 cut(s) 675, 1428, 1939
DraIII CACNNNGTG 1 cut(s) 1107
DriI GACNNNNNGTC 1 cut(s) 2185
EaeI YGGCCR 2 cut(s) 246, 2568
Eam1104I CTCTTC 3 cut(s) 2669, 3054, 3197
Eam1105I GACNNNNNGTC 1 cut(s) 2185
EarI CTCTTC 3 cut(s) 2669, 3054, 3197
EciI GGCGGA 1 cut(s) 3288
Ecl136II GAGCTC 1 cut(s) 144
Eco130I CCWWGG 3 cut(s) 654, 1397, 2228
Eco147I AGGCCT 1 cut(s) 2870
Eco24I GRGCYC 1 cut(s) 146
Eco31I GGTCTC 1 cut(s) 777
Eco32I GATATC 2 cut(s) 2718, 3370
Eco47I GGWCC 3 cut(s) 1961, 2673, 2796
Eco53kI GAGCTC 1 cut(s) 144
Eco57I CTGAAG 2 cut(s) 1655, 2472
Eco88I CYCGRG 1 cut(s) 821
EcoICRI GAGCTC 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 2673
EcoRII CCWGG 8 cut(s) 867, 875, 1161, 1302, 1965, 2014, 2865, 2994
EcoRV GATATC 2 cut(s) 2718, 3370
EcoT14I CCWWGG 3 cut(s) 654, 1397, 2228
EcoT22I ATGCAT 1 cut(s) 3389
EcoT38I GRGCYC 1 cut(s) 146
ErhI CCWWGG 3 cut(s) 654, 1397, 2228
Esp3I CGTCTC 1 cut(s) 3232
FaqI GGGAC 4 cut(s) 1947, 2190, 2257, 2659
FbaI TGATCA 4 cut(s) 1032, 1155, 2776, 2893
FblI GTMKAC 3 cut(s) 171, 1170, 2372
Fnu4HI GCNGC 6 cut(s) 204, 939, 1188, 1928, 2020, 2557
FokI GGATG 9 cut(s) 228, 287, 762, 962, 1433, 1502, 1646, 2214, 2245
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 6 cut(s) 204, 939, 1188, 1928, 2020, 2557
FspBI CTAG 8 cut(s) 494, 767, 1227, 2339, 2376, 2541, 2640, 3143
GlaI GCGC 5 cut(s) 138, 207, 416, 418, 2170
GluI GCNGC 6 cut(s) 204, 939, 1188, 1928, 2020, 2557
GsaI CCCAGC 1 cut(s) 2744
GsuI CTGGAG 2 cut(s) 2849, 3028
HaeIII GGCC 4 cut(s) 248, 2014, 2570, 2870
HapII CCGG 2 cut(s) 245, 3373
HgaI GACGC 1 cut(s) 2621
HhaI GCGC 5 cut(s) 139, 208, 417, 419, 2171
Hin6I GCGC 5 cut(s) 137, 206, 415, 417, 2169
HinP1I GCGC 5 cut(s) 137, 206, 415, 417, 2169
HincII GTYRAC 4 cut(s) 172, 459, 1454, 1884
HindII GTYRAC 4 cut(s) 172, 459, 1454, 1884
HpaII CCGG 2 cut(s) 245, 3373
HphI GGTGA 6 cut(s) 42, 896, 1004, 1708, 2462, 2920
Hpy99I CGWCG 4 cut(s) 173, 176, 188, 191
HpyCH4IV ACGT 3 cut(s) 1297, 1782, 1895
HpySE526I ACGT 3 cut(s) 1297, 1782, 1895
HspAI GCGC 5 cut(s) 137, 206, 415, 417, 2169
Kpn2I TCCGGA 1 cut(s) 3372
Ksp22I TGATCA 4 cut(s) 1032, 1155, 2776, 2893
LmnI GCTCC 7 cut(s) 27, 397, 1348, 1498, 2168, 2991, 3091
Lsp1109I GCAGC 5 cut(s) 925, 1199, 1939, 2006, 2543
LweI GCATC 8 cut(s) 106, 687, 883, 1062, 1480, 1666, 2443, 3232
MabI ACCWGGT 2 cut(s) 1161, 1302
MaeI CTAG 8 cut(s) 494, 767, 1227, 2339, 2376, 2541, 2640, 3143
MaeII ACGT 3 cut(s) 1297, 1782, 1895
MaeIII GTNAC 6 cut(s) 69, 90, 992, 1064, 1298, 1640
MfeI CAATTG 1 cut(s) 2619
MflI RGATCY 7 cut(s) 620, 646, 1010, 1231, 1718, 2080, 2425
MhlI GDGCHC 4 cut(s) 146, 544, 2771, 3247
MlsI TGGCCA 1 cut(s) 2570
MluNI TGGCCA 1 cut(s) 2570
MlyI GAGTC 3 cut(s) 839, 2096, 3326
MmeI TCCRAC 5 cut(s) 49, 143, 1395, 1765, 3189
Mox20I TGGCCA 1 cut(s) 2570
Mph1103I ATGCAT 1 cut(s) 3389
MroI TCCGGA 1 cut(s) 3372
MscI TGGCCA 1 cut(s) 2570
MslI CAYNNNNRTG 6 cut(s) 217, 695, 748, 1871, 2523, 2753
Msp20I TGGCCA 1 cut(s) 2570
MspA1I CMGCKG 1 cut(s) 373
MspCI CTTAAG 2 cut(s) 1498, 1603
MspI CCGG 2 cut(s) 245, 3373
MspR9I CCNGG 8 cut(s) 869, 877, 1163, 1304, 1967, 2016, 2867, 2996
MunI CAATTG 1 cut(s) 2619
Mva1269I GAATGC 4 cut(s) 1345, 1591, 1929, 3389
MvaI CCWGG 8 cut(s) 869, 877, 1163, 1304, 1967, 2016, 2867, 2996
MvnI CGCG 2 cut(s) 137, 417
NcoI CCATGG 2 cut(s) 1397, 2228
NheI GCTAGC 1 cut(s) 493
NlaIV GGNNCC 7 cut(s) 393, 543, 1720, 1963, 2360, 2675, 2770
NmuCI GTSAC 4 cut(s) 69, 90, 992, 1640
NsiI ATGCAT 1 cut(s) 3389
NspI RCATGY 2 cut(s) 2383, 2391
OliI CACNNNNGTG 1 cut(s) 1871
PacI TTAATTAA 1 cut(s) 801
PaeI GCATGC 1 cut(s) 2383
PagI TCATGA 1 cut(s) 1035
PauI GCGCGC 1 cut(s) 415
PceI AGGCCT 1 cut(s) 2870
PciI ACATGT 1 cut(s) 2387
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 3247
PctI GAATGC 4 cut(s) 1345, 1591, 1929, 3389
PfeI GAWTC 9 cut(s) 7, 124, 293, 1145, 1774, 2492, 2846, 3096, 3376
PflFI GACNNNGTC 1 cut(s) 2823
PflMI CCANNNNNTGG 1 cut(s) 2995
PfoI TCCNGGA 1 cut(s) 2994
PkrI GCNGC 6 cut(s) 205, 940, 1189, 1929, 2021, 2558
PleI GAGTC 3 cut(s) 839, 2096, 3326
PpsI GAGTC 3 cut(s) 839, 2096, 3326
Ppu21I YACGTR 1 cut(s) 1298
PpuMI RGGWCCY 1 cut(s) 2673
PscI ACATGT 1 cut(s) 2387
PshBI ATTAAT 2 cut(s) 801, 1242
Psp124BI GAGCTC 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 2673
Psp6I CCWGG 8 cut(s) 867, 875, 1161, 1302, 1965, 2014, 2865, 2994
PspFI CCCAGC 1 cut(s) 2740
PspGI CCWGG 8 cut(s) 867, 875, 1161, 1302, 1965, 2014, 2865, 2994
PspN4I GGNNCC 7 cut(s) 393, 543, 1720, 1963, 2360, 2675, 2770
PspPI GGNCC 4 cut(s) 1961, 2012, 2673, 2796
PspPPI RGGWCCY 1 cut(s) 2673
PstI CTGCAG 1 cut(s) 1920
PsuI RGATCY 7 cut(s) 620, 646, 1010, 1231, 1718, 2080, 2425
PsyI GACNNNGTC 1 cut(s) 2823
PteI GCGCGC 1 cut(s) 415
PvuII CAGCTG 1 cut(s) 373
RsaI GTAC 7 cut(s) 660, 958, 1857, 1894, 2000, 2293, 2392
RsaNI GTAC 7 cut(s) 659, 957, 1856, 1893, 1999, 2292, 2391
RseI CAYNNNNRTG 6 cut(s) 217, 695, 748, 1871, 2523, 2753
SacI GAGCTC 1 cut(s) 146
SalI GTCGAC 1 cut(s) 170
SatI GCNGC 6 cut(s) 204, 939, 1188, 1928, 2020, 2557
Sau96I GGNCC 4 cut(s) 1961, 2012, 2673, 2796
SchI GAGTC 3 cut(s) 839, 2096, 3326
ScrFI CCNGG 8 cut(s) 869, 877, 1163, 1304, 1967, 2016, 2867, 2996
SduI GDGCHC 4 cut(s) 146, 544, 2771, 3247
SexAI ACCWGGT 2 cut(s) 1161, 1302
SfaNI GCATC 8 cut(s) 106, 687, 883, 1062, 1480, 1666, 2443, 3232
SfcI CTRYAG 2 cut(s) 1438, 1916
SgrDI CGTCGACG 1 cut(s) 170
SinI GGWCC 3 cut(s) 1961, 2673, 2796
SmiMI CAYNNNNRTG 6 cut(s) 217, 695, 748, 1871, 2523, 2753
SmlI CTYRAG 5 cut(s) 1281, 1498, 1603, 2732, 2879
SmoI CTYRAG 5 cut(s) 1281, 1498, 1603, 2732, 2879
SpeI ACTAGT 2 cut(s) 2540, 3142
SphI GCATGC 1 cut(s) 2383
SseBI AGGCCT 1 cut(s) 2870
SsiI CCGC 3 cut(s) 203, 760, 3273
SspI AATATT 1 cut(s) 1934
SspMI CTAG 8 cut(s) 494, 767, 1227, 2339, 2376, 2541, 2640, 3143
SstI GAGCTC 1 cut(s) 146
StuI AGGCCT 1 cut(s) 2870
StyD4I CCNGG 8 cut(s) 867, 875, 1161, 1302, 1965, 2014, 2865, 2994
StyI CCWWGG 3 cut(s) 654, 1397, 2228
TaiI ACGT 3 cut(s) 1300, 1785, 1898
TaqI TCGA 4 cut(s) 5, 171, 1557, 3309
TaqII GACCGA 2 cut(s) 1869, 2813
TatI WGTACW 2 cut(s) 1998, 2390
TauI GCSGC 1 cut(s) 206
TfiI GAWTC 9 cut(s) 7, 124, 293, 1145, 1774, 2492, 2846, 3096, 3376
TseFI GTSAC 4 cut(s) 69, 90, 992, 1640
TseI GCWGC 5 cut(s) 938, 1187, 1927, 2019, 2556
Tsp45I GTSAC 4 cut(s) 69, 90, 992, 1640
TspGWI ACGGA 2 cut(s) 149, 1439
Tth111I GACNNNGTC 1 cut(s) 2823
Van91I CCANNNNNTGG 1 cut(s) 2995
Vha464I CTTAAG 2 cut(s) 1498, 1603
VpaK11BI GGWCC 3 cut(s) 1961, 2673, 2796
VspI ATTAAT 2 cut(s) 801, 1242
XapI RAATTY 4 cut(s) 261, 816, 1940, 2476
XceI RCATGY 2 cut(s) 2383, 2391
XcmI CCANNNNNNNNNTGG 1 cut(s) 2733
XmiI GTMKAC 3 cut(s) 171, 1170, 2372
XspI CTAG 8 cut(s) 494, 767, 1227, 2339, 2376, 2541, 2640, 3143
Zsp2I ATGCAT 1 cut(s) 3389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.