Rroxscaffold_2G00153360

Rap1-interacting factor 1 N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006378
Physical Location & Seq
Forward (+)
90129157 .. 90135243
6087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00153360.1

Sequence Viewer

Length: 3432 bp
ATGTCGAATCTCTCAGACCAGCTCCAAGAAATCAAATCCCTAATTTCTTCACCCACCAAATCAAACAAGTCACTCGCGTACTCCACTCTATCACACCTCCAACAACAGTCCTCCGATGCTCTGACTCAAACACTCGCGCTGAGCTCGCAGAGCCTTCTCCATCTGATCGTCGCCGATATTCACGACGACGACGAAGAAATTGCGGCGCAGGCATTGAAATGTTTGGGTTTTATGATATACCATCCGGCCATTGTTTCCGAAATTTCAGTGGAGGATGTGAAGTTGGTTTTGGAATCATTGGTTAAACTCATTACAACTACGAAAATTAAGTCGGTTTGTAATTTAGGCGTGTGGTGCATATCTATACAACAGCTGAGTGAACCACTTTTGGCTCCCCATTTCCATTCTCTTTTGCGCGCAGTAGTTCATGCCATTGACAATCCCATTGGTTCTCTGTCAACAACTTTTGAGGCTATTCAGGCTGTTATTAAGCTAGCAACTCTTTTAAGTGAGAACATGAGAGAGTTATCTCATATATGGGCACCTCCAATATATAGAAGACTGCTTAGCTCTGATAAGAGAGACAGGGATATGTCTGAAAGGTGTCTGTTGAAGATTAGATCCACAATCATACCCCCTCCAATAGATCTCTCCAAGGTACTTGTCAGAGATTTAAAGACAAAGTTGCTCACTATGATGCACGATATGCTAACCAGTGGTATGAAGGTTCAAACTATTCAAGCATGGGGATGGTATATCCGCTTACTAGGGTCTCATGCCTTGAAGAACAGACATTTAATTAATGATTTGCTGAAAATTCCAGAGAAAACATTTTCAGATCACGACTCACAAGTTCAGATTGCTTCCCAGGTTGCCTGGGAAGGTCTTATTGATGCTCTTATTCACCCTCCAATGATATTACCGTGTGCAACAAATGCTGCAAAAGAGGATGATGGTACGCAAAAAAAGGGAACACATAAAGGAAACAGTGGTGACATCCAAAACACTAGATCTTTGAAAAGCGTAAGGCTGATTATGACACCACTGATAGGCATCATGTTGAGTAACTGTGATGTATCTGTCCACATGGCTTGCTTGAACACGTGGTGCTATCTGTTACATAGACTTGATACCTCTCTGAATGATTCTTCAATGATCAACCTGGTAGTAGATCCTATCTTTGAAGCAGCTTTTCAACTTGATCCTGATGGTAAGAGCTTCTGGGCTAGGGATCTGTGCATTAATTTGCTTGATGATTTTATATCAGCAAAATGTAAACCTCAAGACTATGACCCATGTAACCAGGTAAGCCAGCAGTTATCAGCCAAAACCTCTATGAATGCTCCCATTATATCTGGCAGTTGCTCATGGAAGCAGTATCCTATTAAATGGTTGCCATGGGATTTAAGTCTGTTGGACTTTCATTTAAAGATGATCTACAGTCTCATCCATCAACCATCAAGGCAAACTGTCTGCCATGATAACAGGGTTCCAGCTGATGATGCTCCCTTAAGGTTATTTAGAGCTGTTTTGAAAGGAGTTGAACAGGAGTTCAAGAGGTCATCCATCGATTATGATGATATTATGTTGTGTTTGAATGCAGTCTTGAAGTTCTTAAGAAATATGTGTGAAGAACTAAGTCTGGATGGTAGTGACAGAAATGGCTTGCATCATATATCACTTCAGTATATGGAGGCTGTCAGCGAGGTGATAGTACCTACTCTAATGGGATCCCCTCTTTACAAGGTGGCATTGAACATCAAGTATATTGAAAACCTGCAAATGATTCCTGACGTTGGATATGAAAGATCTGGTGTCTGCTCTATTGGTTATATGGACATGGTTTCGCCAATGGTTTATTTAAGCGTACTTTATTTCACTGTGGTGGTTCGGTCAACTTTGAGTACATCCAAAGTCGACTACATTCTGCAGGGAATGCAGCAATATTTTAAATTTATGTTATCATCTTTTGGTCCCCTGGAAAGTCTCGTTATCACAACTGAATTATTGTACAAGCACACAGGGCCAGGCTGCTTAACGATGTGGATAGCAATAGCAGAATGTCTGAAAAACTACATAAATGATGTGAAGGATCTTTCATTGTTCAAAATGGACTCTGAGAGTAAGGGTTGTTATGCCATGAGCCATCTAATGTCCTATCCACTGCTTGTATGCTCCTGCGCCCAGAAAGACATTATGTCAGCAATGAGTGGATGTCCCTCAGAAACCCCTTCTTGTCCACAGATGAATGCTGAGCTTGAACAAGTTATCACATTATGGAAGTCCCTCTATGGTTCTATCTGTACCTCACTGTCTGGGTGTATCATTGTTGGCAGTTTTTCTGAGGCTCTAGTTTCTGTATTGGACAGGTGCCTTGACAAGTATGCTAGCATGCTTACATGTGCTAACGAGCTTCATTTAAACTTCAAGGATTTGGATCTTCATCTCATTTCCTTATATGGTGATGCTTTTATGTGTATTCTGAAAAATTCCAGTTCTTCAGAATCAAGTTCAGATGGAAATGCCAAGCATTGCTATGATTACAAGATCACAAGTGGCATTAAAGGCTGCTTGACTTTGGCCATTAGATATATGAAGTTGCTGCAGACAAAGATAGGAATAGATGCACCAATTGGTCTTACTATGTTCTCTAGTTGCAGGTTATGTTCTGCATTGGCACTCTTCATCAGGTCCCTTCACTTGAAGCAAGATATTGTTTCCTTCTTTGAGATGATATCATGCCCACTGCTTGAGTGGCTGGGATACATGGAAATGCAAGATGAGGGCACTAGTGATCAATTTGAAGTCCTATGGATCGAAATGCTTAATTGTTTAAGACAAAGTCAGCCTCCAATAGTCTTTGATTCTGCATTTCTCAAACTCCAGGCCTCCATCCTTGAGAAAACTCTTGATCACTCAAAACTGTCCATTTCAGAGCCAACCATCACCTTTTGGAACTCCATATATGGCGAACAGATTAACTTGGATTACCCTCAGACCTTGCTTCATGTCCTGGACAAGCTCTGGAGGCAGGGAAGAATAAACCTCCACAAGAGAAGCCTACCTCTTCAAAGATGTCAATCTAAATCGGAAGCCATCACTGCTCCACCGAGATTCACGGTCAATACAGCACACAATAGAGGCTCTAAAAGAGTAGAACTAGTAGAGAATACCACAGACAGTAAGCAGAAGGAAATGCCACATTTGAGTTTGAAAAGGAAGAGGTCGGAACTCACTGAGCATCAGAAGGAAGTGAGACGAGCACAACGAGGAAGGGGAAGGGATTGTGGCGGACACGGCATTGGTATCCAGACTTACACAAGACTCGACTTTTCACAAGGGAATGAGGACTCACAGGGGAATGAGGATTCGCAAGACATCGAAGATATCCGGAATCCAGAATGCATTTTGCAGATGTTGAGGAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04550 map04550
Pfam Domains
Protein Families
Rif1_N

Protein Analysis

1143

Amino Acids

128.7

Weight (kDa)

6.29

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rif1_N PF12231 18 - 301 5.4e-33 Rap1-interacting factor 1 N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1785, 2649
AccB1I GGYRCC 2 cut(s) 541, 2370
AccI GTMKAC 1 cut(s) 1917
AccII CGCG 3 cut(s) 77, 137, 417
AccIII TCCGGA 1 cut(s) 3390
AciI CCGC 3 cut(s) 203, 760, 3291
AclWI GGATC 9 cut(s) 615, 1166, 1196, 1239, 1725, 1738, 2100, 2445, 2822
AcoI YGGCCR 2 cut(s) 246, 2580
AcsI RAATTY 4 cut(s) 261, 816, 1952, 2488
AcuI CTGAAG 2 cut(s) 1667, 2484
AcvI CACGTG 1 cut(s) 1104
AdeI CACNNNGTG 1 cut(s) 1107
AfaI GTAC 8 cut(s) 80, 660, 958, 1716, 1869, 1906, 2012, 2305
AfiI CCNNNNNNNGG 2 cut(s) 1049, 1796
AflII CTTAAG 2 cut(s) 1510, 1615
AflIII ACRYGT 2 cut(s) 1101, 2399
AhdI GACNNNNNGTC 1 cut(s) 2197
AhlI ACTAGT 2 cut(s) 2789, 3160
AjnI CCWGG 8 cut(s) 867, 875, 1161, 1302, 1977, 2026, 2883, 3012
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AleI CACNNNNGTG 1 cut(s) 1883
Alw21I GWGCWC 2 cut(s) 146, 3265
Alw26I GTCTC 5 cut(s) 576, 777, 1448, 1991, 3250
AlwI GGATC 9 cut(s) 615, 1166, 1196, 1239, 1725, 1738, 2100, 2445, 2822
Aor13HI TCCGGA 1 cut(s) 3390
AoxI GGCC 4 cut(s) 246, 2024, 2580, 2886
ApeKI GCWGC 6 cut(s) 938, 1187, 1939, 2031, 2568, 2602
ApoI RAATTY 4 cut(s) 261, 816, 1952, 2488
ArsI GACNNNNNNTTYG 2 cut(s) 314, 346
AseI ATTAAT 2 cut(s) 801, 1242
AspLEI GCGC 5 cut(s) 139, 208, 417, 419, 2183
AspS9I GGNCC 3 cut(s) 1973, 2024, 2691
AsuHPI GGTGA 6 cut(s) 42, 896, 1004, 1720, 2474, 2938
AsuNHI GCTAGC 2 cut(s) 493, 2387
AvaII GGWCC 2 cut(s) 1973, 2691
BaeGI GKGCMC 2 cut(s) 544, 2789
BalI TGGCCA 1 cut(s) 2582
BamHI GGATCC 1 cut(s) 1730
BanI GGYRCC 2 cut(s) 541, 2370
BanII GRGCYC 1 cut(s) 146
BbrPI CACGTG 1 cut(s) 1104
BbsI GAAGAC 1 cut(s) 565
Bbv12I GWGCWC 2 cut(s) 146, 3265
BbvI GCAGC 6 cut(s) 925, 1199, 1951, 2018, 2555, 2589
BceAI ACGGC 1 cut(s) 3313
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
BciVI GTATCC 3 cut(s) 1389, 2756, 3317
BclI TGATCA 3 cut(s) 1155, 2794, 2911
BcoDI GTCTC 5 cut(s) 576, 777, 1448, 1991, 3250
BcuI ACTAGT 2 cut(s) 2789, 3160
BfaI CTAG 9 cut(s) 494, 767, 1008, 1227, 2351, 2388, 2652, 2790, 3161
BfmI CTRYAG 3 cut(s) 1438, 1928, 2603
BfrI CTTAAG 2 cut(s) 1510, 1615
BfuAI ACCTGC 2 cut(s) 1785, 2649
BfuI GTATCC 3 cut(s) 1389, 2756, 3317
BglII AGATCT 3 cut(s) 646, 1010, 1808
BisI GCNGC 7 cut(s) 204, 939, 1188, 1940, 2032, 2569, 2603
BlpI GCTNAGC 3 cut(s) 140, 566, 2253
BlsI GCNGC 7 cut(s) 205, 940, 1189, 1941, 2033, 2570, 2604
Bme1390I CCNGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
Bme18I GGWCC 2 cut(s) 1973, 2691
BmeRI GACNNNNNGTC 1 cut(s) 2197
BmgT120I GGNCC 3 cut(s) 1973, 2024, 2691
BmiI GGNNCC 7 cut(s) 393, 543, 1491, 1732, 1975, 2372, 2693
BmrFI CCNGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
BmsI GCATC 9 cut(s) 106, 687, 883, 1062, 1492, 1678, 2455, 2614, 3250
BmtI GCTAGC 2 cut(s) 497, 2391
BpiI GAAGAC 1 cut(s) 565
BpmI CTGGAG 2 cut(s) 2867, 3046
Bpu1102I GCTNAGC 3 cut(s) 140, 566, 2253
BpuEI CTTGAG 3 cut(s) 1266, 2771, 2918
Bsa29I ATCGAT 1 cut(s) 1569
BsaAI YACGTR 1 cut(s) 1104
BsaBI GATNNNNATC 4 cut(s) 1052, 2436, 2442, 3079
BsaI GGTCTC 1 cut(s) 777
BsaJI CCNNGG 5 cut(s) 654, 867, 876, 1397, 1977
BsaWI WCCGGW 1 cut(s) 3390
Bsc4I CCNNNNNNNGG 2 cut(s) 1049, 1796
Bse1I ACTGG 2 cut(s) 714, 2493
Bse3DI GCAATG 2 cut(s) 2211, 2530
Bse8I GATNNNNATC 4 cut(s) 1052, 2436, 2442, 3079
BseAI TCCGGA 1 cut(s) 3390
BseBI CCWGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
BseCI ATCGAT 1 cut(s) 1569
BseDI CCNNGG 5 cut(s) 654, 867, 876, 1397, 1977
BseJI GATNNNNATC 4 cut(s) 1052, 2436, 2442, 3079
BseLI CCNNNNNNNGG 2 cut(s) 1049, 1796
BseMI GCAATG 2 cut(s) 2211, 2530
BseMII CTCAG 9 cut(s) 27, 131, 365, 2109, 2235, 2244, 2334, 3008, 3228
BseNI ACTGG 2 cut(s) 714, 2493
BsePI GCGCGC 1 cut(s) 415
BseSI GKGCMC 2 cut(s) 544, 2789
BseXI GCAGC 6 cut(s) 925, 1199, 1951, 2018, 2555, 2589
BseYI CCCAGC 1 cut(s) 2758
Bsh1236I CGCG 3 cut(s) 77, 137, 417
BshFI GGCC 4 cut(s) 248, 2026, 2582, 2888
BshNI GGYRCC 2 cut(s) 541, 2370
BshVI ATCGAT 1 cut(s) 1569
BsiHKAI GWGCWC 2 cut(s) 146, 3265
BsiSI CCGG 2 cut(s) 245, 3391
BslFI GGGAC 4 cut(s) 1959, 2202, 2269, 2677
BslI CCNNNNNNNGG 2 cut(s) 1049, 1796
BsmAI GTCTC 5 cut(s) 576, 777, 1448, 1991, 3250
BsmBI CGTCTC 1 cut(s) 3250
BsmFI GGGAC 4 cut(s) 1959, 2202, 2269, 2677
BsmI GAATGC 5 cut(s) 1345, 1603, 1941, 2254, 3407
BsnI GGCC 4 cut(s) 248, 2026, 2582, 2888
Bso31I GGTCTC 1 cut(s) 777
Bsp1286I GDGCHC 4 cut(s) 146, 544, 2789, 3265
Bsp13I TCCGGA 1 cut(s) 3390
Bsp1407I TGTACA 1 cut(s) 2010
Bsp1720I GCTNAGC 3 cut(s) 140, 566, 2253
Bsp19I CCATGG 1 cut(s) 1397
BspACI CCGC 3 cut(s) 203, 760, 3291
BspANI GGCC 4 cut(s) 248, 2026, 2582, 2888
BspCNI CTCAG 9 cut(s) 26, 132, 366, 2110, 2234, 2245, 2335, 3007, 3229
BspDI ATCGAT 1 cut(s) 1569
BspEI TCCGGA 1 cut(s) 3390
BspFNI CGCG 3 cut(s) 77, 137, 417
BspLI GGNNCC 7 cut(s) 393, 543, 1491, 1732, 1975, 2372, 2693
BspMAI CTGCAG 2 cut(s) 1932, 2607
BspMI ACCTGC 2 cut(s) 1785, 2649
BspOI GCTAGC 2 cut(s) 497, 2391
BspPI GGATC 9 cut(s) 615, 1166, 1196, 1239, 1725, 1738, 2100, 2445, 2822
BspT107I GGYRCC 2 cut(s) 541, 2370
BspTI CTTAAG 2 cut(s) 1510, 1615
BspTNI GGTCTC 1 cut(s) 777
BsrDI GCAATG 2 cut(s) 2211, 2530
BsrGI TGTACA 1 cut(s) 2010
BsrI ACTGG 2 cut(s) 714, 2493
BssECI CCNNGG 5 cut(s) 654, 867, 876, 1397, 1977
BssHII GCGCGC 1 cut(s) 415
BssT1I CCWWGG 2 cut(s) 654, 1397
Bst2UI CCWGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
Bst6I CTCTTC 3 cut(s) 2687, 3072, 3215
BstAFI CTTAAG 2 cut(s) 1510, 1615
BstAPI GCANNNNNTGC 3 cut(s) 706, 935, 1936
BstAUI TGTACA 1 cut(s) 2010
BstBAI YACGTR 1 cut(s) 1104
BstC8I GCNNGC 9 cut(s) 146, 210, 417, 495, 1093, 1313, 1667, 2389, 2393
BstDSI CCRYGG 1 cut(s) 1397
BstFNI CGCG 3 cut(s) 77, 137, 417
BstHHI GCGC 5 cut(s) 139, 208, 417, 419, 2183
BstMAI GTCTC 5 cut(s) 576, 777, 1448, 1991, 3250
BstNI CCWGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
BstNSI RCATGY 2 cut(s) 2395, 2403
BstSCI CCNGG 8 cut(s) 867, 875, 1161, 1302, 1977, 2026, 2883, 3012
BstSFI CTRYAG 3 cut(s) 1438, 1928, 2603
BstSLI GKGCMC 2 cut(s) 544, 2789
BstUI CGCG 3 cut(s) 77, 137, 417
BstV1I GCAGC 6 cut(s) 925, 1199, 1951, 2018, 2555, 2589
BstV2I GAAGAC 1 cut(s) 565
BstX2I RGATCY 9 cut(s) 620, 646, 1010, 1171, 1231, 1730, 1808, 2092, 2437
BstYI RGATCY 9 cut(s) 620, 646, 1010, 1171, 1231, 1730, 1808, 2092, 2437
Bsu15I ATCGAT 1 cut(s) 1569
BsuI GTATCC 3 cut(s) 1389, 2756, 3317
BsuRI GGCC 4 cut(s) 248, 2026, 2582, 2888
BsuTUI ATCGAT 1 cut(s) 1569
BtgI CCRYGG 1 cut(s) 1397
BtsI GCAGTG 3 cut(s) 2162, 2744, 3099
BveI ACCTGC 2 cut(s) 1785, 2649
Cac8I GCNNGC 9 cut(s) 146, 210, 417, 495, 1093, 1313, 1667, 2389, 2393
CfoI GCGC 5 cut(s) 139, 208, 417, 419, 2183
Cfr13I GGNCC 3 cut(s) 1973, 2024, 2691
ClaI ATCGAT 1 cut(s) 1569
CsiI ACCWGGT 2 cut(s) 1161, 1302
Csp6I GTAC 8 cut(s) 79, 659, 957, 1715, 1868, 1905, 2011, 2304
CspCI CAANNNNNGTGG 2 cut(s) 1073, 1108
CviQI GTAC 8 cut(s) 79, 659, 957, 1715, 1868, 1905, 2011, 2304
DraI TTTAAA 4 cut(s) 675, 1428, 1951, 2421
DraIII CACNNNGTG 1 cut(s) 1107
DriI GACNNNNNGTC 1 cut(s) 2197
EaeI YGGCCR 2 cut(s) 246, 2580
Eam1104I CTCTTC 3 cut(s) 2687, 3072, 3215
Eam1105I GACNNNNNGTC 1 cut(s) 2197
EarI CTCTTC 3 cut(s) 2687, 3072, 3215
EciI GGCGGA 1 cut(s) 3306
Ecl136II GAGCTC 1 cut(s) 144
Eco130I CCWWGG 2 cut(s) 654, 1397
Eco147I AGGCCT 1 cut(s) 2888
Eco24I GRGCYC 1 cut(s) 146
Eco31I GGTCTC 1 cut(s) 777
Eco32I GATATC 2 cut(s) 2736, 3388
Eco47I GGWCC 2 cut(s) 1973, 2691
Eco53kI GAGCTC 1 cut(s) 144
Eco57I CTGAAG 2 cut(s) 1667, 2484
Eco72I CACGTG 1 cut(s) 1104
EcoICRI GAGCTC 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 2691
EcoRII CCWGG 8 cut(s) 867, 875, 1161, 1302, 1977, 2026, 2883, 3012
EcoRV GATATC 2 cut(s) 2736, 3388
EcoT14I CCWWGG 2 cut(s) 654, 1397
EcoT22I ATGCAT 1 cut(s) 3407
EcoT38I GRGCYC 1 cut(s) 146
ErhI CCWWGG 2 cut(s) 654, 1397
Esp3I CGTCTC 1 cut(s) 3250
FaqI GGGAC 4 cut(s) 1959, 2202, 2269, 2677
FbaI TGATCA 3 cut(s) 1155, 2794, 2911
FblI GTMKAC 1 cut(s) 1917
Fnu4HI GCNGC 7 cut(s) 204, 939, 1188, 1940, 2032, 2569, 2603
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 7 cut(s) 204, 939, 1188, 1940, 2032, 2569, 2603
FspBI CTAG 9 cut(s) 494, 767, 1008, 1227, 2351, 2388, 2652, 2790, 3161
GlaI GCGC 5 cut(s) 138, 207, 416, 418, 2182
GluI GCNGC 7 cut(s) 204, 939, 1188, 1940, 2032, 2569, 2603
GsaI CCCAGC 1 cut(s) 2762
GsuI CTGGAG 2 cut(s) 2867, 3046
HaeIII GGCC 4 cut(s) 248, 2026, 2582, 2888
HapII CCGG 2 cut(s) 245, 3391
HhaI GCGC 5 cut(s) 139, 208, 417, 419, 2183
Hin6I GCGC 5 cut(s) 137, 206, 415, 417, 2181
HinP1I GCGC 5 cut(s) 137, 206, 415, 417, 2181
HincII GTYRAC 3 cut(s) 459, 1896, 1918
HindII GTYRAC 3 cut(s) 459, 1896, 1918
HpaII CCGG 2 cut(s) 245, 3391
HphI GGTGA 6 cut(s) 42, 896, 1004, 1720, 2474, 2938
Hpy166II GTNNAC 7 cut(s) 380, 459, 1084, 1277, 1896, 1918, 2240
Hpy8I GTNNAC 7 cut(s) 380, 459, 1084, 1277, 1896, 1918, 2240
Hpy99I CGWCG 4 cut(s) 173, 188, 191, 194
HpyCH4IV ACGT 2 cut(s) 1103, 1794
HpySE526I ACGT 2 cut(s) 1103, 1794
HspAI GCGC 5 cut(s) 137, 206, 415, 417, 2181
Kpn2I TCCGGA 1 cut(s) 3390
Ksp22I TGATCA 3 cut(s) 1155, 2794, 2911
LmnI GCTCC 6 cut(s) 27, 397, 1348, 1510, 2180, 3109
Lsp1109I GCAGC 6 cut(s) 925, 1199, 1951, 2018, 2555, 2589
LweI GCATC 9 cut(s) 106, 687, 883, 1062, 1492, 1678, 2455, 2614, 3250
MabI ACCWGGT 2 cut(s) 1161, 1302
MaeI CTAG 9 cut(s) 494, 767, 1008, 1227, 2351, 2388, 2652, 2790, 3161
MaeII ACGT 2 cut(s) 1103, 1794
MaeIII GTNAC 6 cut(s) 69, 992, 1064, 1116, 1298, 1652
MfeI CAATTG 1 cut(s) 2631
MflI RGATCY 9 cut(s) 620, 646, 1010, 1171, 1231, 1730, 1808, 2092, 2437
MhlI GDGCHC 4 cut(s) 146, 544, 2789, 3265
MlsI TGGCCA 1 cut(s) 2582
MluNI TGGCCA 1 cut(s) 2582
MlyI GAGTC 5 cut(s) 118, 839, 2108, 3318, 3344
MmeI TCCRAC 4 cut(s) 124, 1395, 1777, 3207
Mox20I TGGCCA 1 cut(s) 2582
Mph1103I ATGCAT 1 cut(s) 3407
MroI TCCGGA 1 cut(s) 3390
MscI TGGCCA 1 cut(s) 2582
MslI CAYNNNNRTG 6 cut(s) 217, 695, 748, 1883, 2535, 2771
Msp20I TGGCCA 1 cut(s) 2582
MspA1I CMGCKG 2 cut(s) 373, 1496
MspCI CTTAAG 2 cut(s) 1510, 1615
MspI CCGG 2 cut(s) 245, 3391
MspR9I CCNGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
MunI CAATTG 1 cut(s) 2631
Mva1269I GAATGC 5 cut(s) 1345, 1603, 1941, 2254, 3407
MvaI CCWGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
MvnI CGCG 3 cut(s) 77, 137, 417
NcoI CCATGG 1 cut(s) 1397
NheI GCTAGC 2 cut(s) 493, 2387
NlaIV GGNNCC 7 cut(s) 393, 543, 1491, 1732, 1975, 2372, 2693
NmuCI GTSAC 3 cut(s) 69, 992, 1652
NsiI ATGCAT 1 cut(s) 3407
NspI RCATGY 2 cut(s) 2395, 2403
OliI CACNNNNGTG 1 cut(s) 1883
PacI TTAATTAA 1 cut(s) 801
PaeI GCATGC 1 cut(s) 2395
PauI GCGCGC 1 cut(s) 415
PceI AGGCCT 1 cut(s) 2888
PciI ACATGT 1 cut(s) 2399
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 3265
PctI GAATGC 5 cut(s) 1345, 1603, 1941, 2254, 3407
PfeI GAWTC 9 cut(s) 7, 293, 1145, 1786, 2504, 2864, 3114, 3368, 3394
PflFI GACNNNGTC 1 cut(s) 2841
PfoI TCCNGGA 1 cut(s) 3012
PkrI GCNGC 7 cut(s) 205, 940, 1189, 1941, 2033, 2570, 2604
PleI GAGTC 5 cut(s) 118, 839, 2108, 3318, 3344
PmaCI CACGTG 1 cut(s) 1104
PmlI CACGTG 1 cut(s) 1104
PpsI GAGTC 5 cut(s) 118, 839, 2108, 3318, 3344
Ppu21I YACGTR 1 cut(s) 1104
PpuMI RGGWCCY 1 cut(s) 2691
PscI ACATGT 1 cut(s) 2399
PshBI ATTAAT 2 cut(s) 801, 1242
Psp124BI GAGCTC 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 2691
Psp6I CCWGG 8 cut(s) 867, 875, 1161, 1302, 1977, 2026, 2883, 3012
PspCI CACGTG 1 cut(s) 1104
PspFI CCCAGC 1 cut(s) 2758
PspGI CCWGG 8 cut(s) 867, 875, 1161, 1302, 1977, 2026, 2883, 3012
PspN4I GGNNCC 7 cut(s) 393, 543, 1491, 1732, 1975, 2372, 2693
PspPI GGNCC 3 cut(s) 1973, 2024, 2691
PspPPI RGGWCCY 1 cut(s) 2691
PstI CTGCAG 2 cut(s) 1932, 2607
PsuI RGATCY 9 cut(s) 620, 646, 1010, 1171, 1231, 1730, 1808, 2092, 2437
PsyI GACNNNGTC 1 cut(s) 2841
PteI GCGCGC 1 cut(s) 415
PvuII CAGCTG 2 cut(s) 373, 1496
RsaI GTAC 8 cut(s) 80, 660, 958, 1716, 1869, 1906, 2012, 2305
RsaNI GTAC 8 cut(s) 79, 659, 957, 1715, 1868, 1905, 2011, 2304
RseI CAYNNNNRTG 6 cut(s) 217, 695, 748, 1883, 2535, 2771
SacI GAGCTC 1 cut(s) 146
SalI GTCGAC 1 cut(s) 1916
SatI GCNGC 7 cut(s) 204, 939, 1188, 1940, 2032, 2569, 2603
Sau96I GGNCC 3 cut(s) 1973, 2024, 2691
SchI GAGTC 5 cut(s) 118, 839, 2108, 3318, 3344
ScrFI CCNGG 8 cut(s) 869, 877, 1163, 1304, 1979, 2028, 2885, 3014
SduI GDGCHC 4 cut(s) 146, 544, 2789, 3265
SexAI ACCWGGT 2 cut(s) 1161, 1302
SfaNI GCATC 9 cut(s) 106, 687, 883, 1062, 1492, 1678, 2455, 2614, 3250
SfcI CTRYAG 3 cut(s) 1438, 1928, 2603
SinI GGWCC 2 cut(s) 1973, 2691
SmiMI CAYNNNNRTG 6 cut(s) 217, 695, 748, 1883, 2535, 2771
SmlI CTYRAG 5 cut(s) 1281, 1510, 1615, 2750, 2897
SmoI CTYRAG 5 cut(s) 1281, 1510, 1615, 2750, 2897
SpeI ACTAGT 2 cut(s) 2789, 3160
SphI GCATGC 1 cut(s) 2395
SseBI AGGCCT 1 cut(s) 2888
SsiI CCGC 3 cut(s) 203, 760, 3291
SspI AATATT 1 cut(s) 1946
SspMI CTAG 9 cut(s) 494, 767, 1008, 1227, 2351, 2388, 2652, 2790, 3161
SstI GAGCTC 1 cut(s) 146
StuI AGGCCT 1 cut(s) 2888
StyD4I CCNGG 8 cut(s) 867, 875, 1161, 1302, 1977, 2026, 2883, 3012
StyI CCWWGG 2 cut(s) 654, 1397
TaiI ACGT 2 cut(s) 1106, 1797
TaqI TCGA 6 cut(s) 5, 1569, 1917, 2817, 3327, 3381
TaqII GACCGA 1 cut(s) 1881
TatI WGTACW 2 cut(s) 1904, 2010
TauI GCSGC 1 cut(s) 206
TfiI GAWTC 9 cut(s) 7, 293, 1145, 1786, 2504, 2864, 3114, 3368, 3394
TseFI GTSAC 3 cut(s) 69, 992, 1652
TseI GCWGC 6 cut(s) 938, 1187, 1939, 2031, 2568, 2602
Tsp45I GTSAC 3 cut(s) 69, 992, 1652
Tth111I GACNNNGTC 1 cut(s) 2841
Vha464I CTTAAG 2 cut(s) 1510, 1615
VpaK11BI GGWCC 2 cut(s) 1973, 2691
VspI ATTAAT 2 cut(s) 801, 1242
XapI RAATTY 4 cut(s) 261, 816, 1952, 2488
XceI RCATGY 2 cut(s) 2395, 2403
XcmI CCANNNNNNNNNTGG 1 cut(s) 2751
XmiI GTMKAC 1 cut(s) 1917
XspI CTAG 9 cut(s) 494, 767, 1008, 1227, 2351, 2388, 2652, 2790, 3161
Zsp2I ATGCAT 1 cut(s) 3407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.