FvH4_1g05730

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004018
Physical Location & Seq
Reverse (-)
3018467 .. 3021482
3016 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g05730.t2

Sequence Viewer

Length: 876 bp
ATGGGGGTGGTTGCGAATGCAGCACAAGCGAGTACAACAGCTTCGCCGGAGAATGTGCTAGAGTGGGTCAAGCAGGACAAACGGAGGCTTCTCCATGTTGTATACCGCGTCGGGGATTTGGACAGGACCATAAAATTCTACACCGAGTGCCTGGGAATGAAATTGCTGCGGAAACGTGACATACCAGAGGAAAGATATACAAATGCTTTTCTTGGATATGGGCCAGAGGATTCCCATTTCGTCATCGAACTCACTTACAATTATGGGGTAGACAAGTATGATATCGGAACTGCTTTCGGTCATTTTGGTGTTGCTGTTGAGGATGTTGCTAAGACTGTGGAACTAGTAAAAGCTAAGGGAGGCAAGGTAACCCGAGAACCTGGTCCAGTGAAAGGTGGAAATACAGTAATAGCTTTTGTTGAAGATCCTGATGGTTATAAGTTTGAACTCTTGGAAAGAGGTCCTACACCTGAACCCTTGTGTCAAGTTATGCTTCGTGTAGGTGATCTTGATCGTTCCATAGCTTTTTATGAGAAGGCTTTTGGCATGGAGCTTCTTCGCAAGAGAGATAATCCAGAGTACAAGTATACTATAGCAATGCTCGGGTATGGCCCTGAAGACAAAAATGCTGTTCTGGAGTTGACATACAACTATGGGGTCACTGAATACAACAAAGGAAATGCTTATGCGCAGATAGCGGTAGGCACAGATGATGTCTATAAAACTGCTGAAGCAATTAAACTCTGCGGAGGCAAGATTACCCGGGAACCTGGGCCATTACCTGGTCTTAACACAAAGATCACTGCTTGCTTGGACCCTGATGGTTGGAAGTCGGTTTTTGTGGATAATGTTGATTTCCTAAAGGAATTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

292

Amino Acids

32.45

Weight (kDa)

5.27

Isoelectric Point (pI)

28.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 99 - 220 2.8e-27 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 102 - 209 1.1e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 232 - 268 3.4e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 438
Acc16I TGCGCA 1 cut(s) 690
AccB7I CCANNNNNTGG 1 cut(s) 782
AccI GTMKAC 3 cut(s) 102, 270, 587
AccII CGCG 1 cut(s) 108
AciI CCGC 4 cut(s) 106, 169, 698, 747
AclWI GGATC 1 cut(s) 419
AcsI RAATTY 1 cut(s) 134
AcuI CTGAAG 2 cut(s) 636, 750
AdeI CACNNNGTG 1 cut(s) 147
AfaI GTAC 2 cut(s) 34, 581
AfiI CCNNNNNNNGG 3 cut(s) 112, 392, 782
AgsI TTSAA 2 cut(s) 422, 446
AhlI ACTAGT 1 cut(s) 343
AjnI CCWGG 4 cut(s) 150, 379, 769, 781
AluBI AGCT 5 cut(s) 41, 353, 413, 524, 553
AluI AGCT 5 cut(s) 41, 353, 413, 524, 553
AlwI GGATC 1 cut(s) 419
Ama87I CYCGRG 3 cut(s) 372, 602, 762
AoxI GGCC 3 cut(s) 221, 610, 773
ApeKI GCWGC 2 cut(s) 20, 166
ApoI RAATTY 1 cut(s) 134
AspLEI GCGC 1 cut(s) 691
AspS9I GGNCC 7 cut(s) 126, 221, 383, 461, 611, 773, 814
AsuC2I CCSGG 2 cut(s) 763, 764
AsuHPI GGTGA 1 cut(s) 515
AvaI CYCGRG 3 cut(s) 372, 602, 762
AvaII GGWCC 4 cut(s) 126, 383, 461, 814
BarI GAAGNNNNNNTAC 2 cut(s) 25, 57
BbsI GAAGAC 1 cut(s) 624
BbvI GCAGC 2 cut(s) 32, 153
BccI CCATC 2 cut(s) 425, 815
BciT130I CCWGG 4 cut(s) 152, 381, 771, 783
BcnI CCSGG 2 cut(s) 763, 764
BcuI ACTAGT 1 cut(s) 343
BfaI CTAG 2 cut(s) 59, 344
BfmI CTRYAG 1 cut(s) 591
BisI GCNGC 2 cut(s) 21, 167
BlsI GCNGC 2 cut(s) 22, 168
Bme1390I CCNGG 6 cut(s) 152, 381, 763, 764, 771, 783
Bme18I GGWCC 4 cut(s) 126, 383, 461, 814
BmeT110I CYCGRG 3 cut(s) 372, 602, 762
BmgT120I GGNCC 7 cut(s) 126, 221, 383, 461, 611, 773, 814
BmiI GGNNCC 2 cut(s) 768, 816
BmrFI CCNGG 6 cut(s) 152, 381, 763, 764, 771, 783
BpiI GAAGAC 1 cut(s) 624
BpmI CTGGAG 1 cut(s) 656
Bpu10I CCTNAGC 1 cut(s) 354
BpuMI CCSGG 2 cut(s) 763, 764
BsaBI GATNNNNATC 1 cut(s) 510
BsaJI CCNNGG 3 cut(s) 151, 762, 770
BsaXI ACNNNNNCTCC 2 cut(s) 351, 381
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 392, 782
Bse1I ACTGG 1 cut(s) 386
Bse3DI GCAATG 1 cut(s) 603
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 4 cut(s) 152, 381, 771, 783
BseDI CCNNGG 3 cut(s) 151, 762, 770
BseGI GGATG 1 cut(s) 328
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 3 cut(s) 112, 392, 782
BseMI GCAATG 1 cut(s) 603
BseNI ACTGG 1 cut(s) 386
BseXI GCAGC 2 cut(s) 32, 153
Bsh1236I CGCG 1 cut(s) 108
BshFI GGCC 3 cut(s) 223, 612, 775
BsiHKCI CYCGRG 3 cut(s) 372, 602, 762
BsiSI CCGG 2 cut(s) 47, 763
BslI CCNNNNNNNGG 3 cut(s) 112, 392, 782
BsmI GAATGC 1 cut(s) 22
BsnI GGCC 3 cut(s) 223, 612, 775
BsoBI CYCGRG 3 cut(s) 372, 602, 762
Bsp143I GATC 4 cut(s) 424, 505, 511, 798
BspACI CCGC 4 cut(s) 106, 169, 698, 747
BspANI GGCC 3 cut(s) 223, 612, 775
BspFNI CGCG 1 cut(s) 108
BspLI GGNNCC 2 cut(s) 768, 816
BspPI GGATC 1 cut(s) 419
BsrDI GCAATG 1 cut(s) 603
BsrI ACTGG 1 cut(s) 386
BssECI CCNNGG 3 cut(s) 151, 762, 770
BssMI GATC 4 cut(s) 424, 505, 511, 798
BssNAI GTATAC 2 cut(s) 103, 588
Bst1107I GTATAC 2 cut(s) 103, 588
Bst2UI CCWGG 4 cut(s) 152, 381, 771, 783
Bst4CI ACNGT 2 cut(s) 337, 406
BstC8I GCNNGC 1 cut(s) 808
BstDEI CTNAG 2 cut(s) 330, 354
BstEII GGTNACC 1 cut(s) 367
BstF5I GGATG 1 cut(s) 328
BstFNI CGCG 1 cut(s) 108
BstHHI GCGC 1 cut(s) 691
BstKTI GATC 4 cut(s) 427, 508, 514, 801
BstMBI GATC 4 cut(s) 424, 505, 511, 798
BstMWI GCNNNNNNNGC 3 cut(s) 20, 26, 695
BstNI CCWGG 4 cut(s) 152, 381, 771, 783
BstPI GGTNACC 1 cut(s) 367
BstSCI CCNGG 6 cut(s) 150, 379, 761, 762, 769, 781
BstSFI CTRYAG 1 cut(s) 591
BstUI CGCG 1 cut(s) 108
BstV1I GCAGC 2 cut(s) 32, 153
BstV2I GAAGAC 1 cut(s) 624
BstX2I RGATCY 1 cut(s) 424
BstYI RGATCY 1 cut(s) 424
BstZ17I GTATAC 2 cut(s) 103, 588
BsuRI GGCC 3 cut(s) 223, 612, 775
BtsCI GGATG 1 cut(s) 328
BtsI GCAGTG 1 cut(s) 801
BtsIMutI CAGTG 3 cut(s) 393, 660, 801
Cac8I GCNNGC 1 cut(s) 808
CfoI GCGC 1 cut(s) 691
Cfr13I GGNCC 7 cut(s) 126, 221, 383, 461, 611, 773, 814
Cfr9I CCCGGG 1 cut(s) 762
CseI GACGC 1 cut(s) 97
CsiI ACCWGGT 2 cut(s) 379, 781
Csp6I GTAC 2 cut(s) 33, 580
CviAII CATG 2 cut(s) 95, 547
CviQI GTAC 2 cut(s) 33, 580
DdeI CTNAG 2 cut(s) 330, 354
DpnI GATC 4 cut(s) 426, 507, 513, 800
DpnII GATC 4 cut(s) 424, 505, 511, 798
DraIII CACNNNGTG 1 cut(s) 147
Eco32I GATATC 1 cut(s) 283
Eco47I GGWCC 4 cut(s) 126, 383, 461, 814
Eco57I CTGAAG 2 cut(s) 636, 750
Eco88I CYCGRG 3 cut(s) 372, 602, 762
Eco91I GGTNACC 1 cut(s) 367
EcoO109I RGGNCCY 1 cut(s) 461
EcoO65I GGTNACC 1 cut(s) 367
EcoRII CCWGG 4 cut(s) 150, 379, 769, 781
EcoRV GATATC 1 cut(s) 283
FaeI CATG 2 cut(s) 98, 550
FalI AAGNNNNNCTT 2 cut(s) 477, 509
FatI CATG 2 cut(s) 94, 546
FblI GTMKAC 3 cut(s) 102, 270, 587
Fnu4HI GCNGC 2 cut(s) 21, 167
FokI GGATG 1 cut(s) 335
Fsp4HI GCNGC 2 cut(s) 21, 167
FspBI CTAG 2 cut(s) 59, 344
FspI TGCGCA 1 cut(s) 690
GlaI GCGC 1 cut(s) 690
GluI GCNGC 2 cut(s) 21, 167
GsuI CTGGAG 1 cut(s) 656
HaeIII GGCC 3 cut(s) 223, 612, 775
HapII CCGG 2 cut(s) 47, 763
HgaI GACGC 1 cut(s) 97
HhaI GCGC 1 cut(s) 691
Hin1II CATG 2 cut(s) 98, 550
Hin6I GCGC 1 cut(s) 689
HinP1I GCGC 1 cut(s) 689
HincII GTYRAC 1 cut(s) 642
HindII GTYRAC 1 cut(s) 642
HinfI GANTC 1 cut(s) 230
HpaII CCGG 2 cut(s) 47, 763
HphI GGTGA 1 cut(s) 515
Hpy166II GTNNAC 4 cut(s) 103, 271, 588, 642
Hpy188I TCNGA 1 cut(s) 287
Hpy188III TCNNGA 4 cut(s) 428, 509, 575, 635
Hpy8I GTNNAC 4 cut(s) 103, 271, 588, 642
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 1 cut(s) 529
HpyCH4III ACNGT 2 cut(s) 337, 406
HpyCH4IV ACGT 1 cut(s) 175
HpyCH4V TGCA 1 cut(s) 20
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 26, 695
HpyF3I CTNAG 2 cut(s) 330, 354
HpySE526I ACGT 1 cut(s) 175
Hsp92II CATG 2 cut(s) 98, 550
HspAI GCGC 1 cut(s) 689
Kzo9I GATC 4 cut(s) 424, 505, 511, 798
LmnI GCTCC 1 cut(s) 550
Lsp1109I GCAGC 2 cut(s) 32, 153
MabI ACCWGGT 2 cut(s) 379, 781
MaeI CTAG 2 cut(s) 59, 344
MaeII ACGT 1 cut(s) 175
MaeIII GTNAC 3 cut(s) 176, 367, 658
MalI GATC 4 cut(s) 426, 507, 513, 800
MboI GATC 4 cut(s) 424, 505, 511, 798
MboII GAAGA 3 cut(s) 434, 548, 629
MflI RGATCY 1 cut(s) 424
MluCI AATT 5 cut(s) 134, 161, 259, 735, 866
MmeI TCCRAC 1 cut(s) 806
MnlI CCTC 7 cut(s) 78, 181, 220, 313, 353, 452, 743
MseI TTAA 2 cut(s) 738, 789
MslI CAYNNNNRTG 1 cut(s) 306
MspI CCGG 2 cut(s) 47, 763
MspR9I CCNGG 6 cut(s) 152, 381, 763, 764, 771, 783
Mva1269I GAATGC 1 cut(s) 22
MvaI CCWGG 4 cut(s) 152, 381, 771, 783
MvnI CGCG 1 cut(s) 108
MwoI GCNNNNNNNGC 3 cut(s) 20, 26, 695
NciI CCSGG 2 cut(s) 763, 764
NdeII GATC 4 cut(s) 424, 505, 511, 798
NlaIII CATG 2 cut(s) 98, 550
NlaIV GGNNCC 2 cut(s) 768, 816
NmuCI GTSAC 2 cut(s) 176, 658
NsbI TGCGCA 1 cut(s) 690
PctI GAATGC 1 cut(s) 22
PfeI GAWTC 1 cut(s) 230
PflMI CCANNNNNTGG 1 cut(s) 782
PkrI GCNGC 2 cut(s) 22, 168
PpuMI RGGWCCY 1 cut(s) 461
PsiI TTATAA 1 cut(s) 438
Psp5II RGGWCCY 1 cut(s) 461
Psp6I CCWGG 4 cut(s) 150, 379, 769, 781
PspEI GGTNACC 1 cut(s) 367
PspGI CCWGG 4 cut(s) 150, 379, 769, 781
PspN4I GGNNCC 2 cut(s) 768, 816
PspPI GGNCC 7 cut(s) 126, 221, 383, 461, 611, 773, 814
PspPPI RGGWCCY 1 cut(s) 461
PsuI RGATCY 1 cut(s) 424
RsaI GTAC 2 cut(s) 34, 581
RsaNI GTAC 2 cut(s) 33, 580
RseI CAYNNNNRTG 1 cut(s) 306
SaqAI TTAA 2 cut(s) 738, 789
SatI GCNGC 2 cut(s) 21, 167
Sau3AI GATC 4 cut(s) 424, 505, 511, 798
Sau96I GGNCC 7 cut(s) 126, 221, 383, 461, 611, 773, 814
ScrFI CCNGG 6 cut(s) 152, 381, 763, 764, 771, 783
SexAI ACCWGGT 2 cut(s) 379, 781
SfcI CTRYAG 1 cut(s) 591
SinI GGWCC 4 cut(s) 126, 383, 461, 814
SmaI CCCGGG 1 cut(s) 764
SmiMI CAYNNNNRTG 1 cut(s) 306
SpeI ACTAGT 1 cut(s) 343
Sse9I AATT 5 cut(s) 134, 161, 259, 735, 866
SsiI CCGC 4 cut(s) 106, 169, 698, 747
SspMI CTAG 2 cut(s) 59, 344
StyD4I CCNGG 6 cut(s) 150, 379, 761, 762, 769, 781
TaaI ACNGT 2 cut(s) 337, 406
TaiI ACGT 1 cut(s) 178
TaqI TCGA 1 cut(s) 246
TaqII GACCGA 1 cut(s) 287
TasI AATT 5 cut(s) 134, 161, 259, 735, 866
TatI WGTACW 2 cut(s) 32, 579
TfiI GAWTC 1 cut(s) 230
Tru1I TTAA 2 cut(s) 738, 789
Tru9I TTAA 2 cut(s) 738, 789
TscAI CASTG 3 cut(s) 393, 667, 808
TseFI GTSAC 2 cut(s) 176, 658
TseI GCWGC 2 cut(s) 20, 166
Tsp45I GTSAC 2 cut(s) 176, 658
TspDTI ATGAA 1 cut(s) 173
TspGWI ACGGA 1 cut(s) 97
TspMI CCCGGG 1 cut(s) 762
TspRI CASTG 3 cut(s) 393, 667, 808
Van91I CCANNNNNTGG 1 cut(s) 782
VpaK11BI GGWCC 4 cut(s) 126, 383, 461, 814
XapI RAATTY 1 cut(s) 134
XmaI CCCGGG 1 cut(s) 762
XmiI GTMKAC 3 cut(s) 102, 270, 587
XspI CTAG 2 cut(s) 59, 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.