MD15G1094500.v1.1

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004018
Physical Location & Seq
Forward (+)
6625341 .. 6628750
3410 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1094500.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGTCATCTTTATCGGCTACTCTGAATCTCTTAAGACCCTCTCTGGCTCCATCGCCACCGTCCGTGAGGTTTTTTGGTGTGCGAAACTCTGCCTCTTCTTATTCTACTTCTCGGAGACTCACTCTCTTTCAACTCGGCTCTGCTCTGCCTCAATCACAACTGTTAGGTGCAAAAGCATCTGAGTTGCTAAGAGGAGAGGGAAACCCTTCAGAGGCTGCCCCAGTTGCGAATTTGGCACAGGCAGGCACCGCTATTGCCAAGGAAAATGCACTGGAGTGGGTAAAAAATGACAAAAGAAGAATGCTTCATGTTGTCTATCGTGTTGGGGACTTGGACAAGACTATAAAATTCTATACTGAATGTTTGGGGATGAAGCTGTTAAGAAAACGTGACATACCTGAGGATCGATATTCGAATGCTTTTCTTGGATATGGACCAGAAGAATCCAATTTTGTGGTTGAACTTACATACAATTACGGAGTGGACAAGTACAACATTGGGACTGGTTTTGGCCATTTTGGCATTGCACTTGAAGATGTAACCAAAACCGTGGAACTTATAAAGGCAAAGGGAGGGAAAGTTACCGAGGAACCTGGTCCTGCTAAAGGTGGCAGCACAATAGTTGCCTCTGTTGAGGATCCCGATGGCTACACGTTTGAGCTTTTGGAGAGAGGGCCTTCACCTGAGCCCTTCCGTCAAGTGATGCTTCGTGTAGGTGATCTTGACCGTGCTATTAACTTTTATAAGAAGGCTTATGGGATGGAACTTCTCCACAAGAGAGATAATCCTGAATACAAGTGTACTGTTGCCGTGTTGGGCTACGGTCCTGAAGATAAGAACGCAGTGCTTGAACTGACGTACAACTATGGGATCACAGATTATGACAAAGGAAATGGTTACGCGCAGATCGCAATAGGCACAGATGATGTTTACAAGACTGCAGAAGCAATCAAACTAAGTGGAGGGAAGATTATTCTTGATCCTGGGCCTTTGCCAGGCATCAACACAAAGATAACTGCTTGCCTCGACCCTGATGGCTGGAAATCGGTCTTTGTTGATAATGCTGATTTTCTGAAGGAATTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

363

Amino Acids

39.61

Weight (kDa)

5.67

Isoelectric Point (pI)

38.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 101 - 221 1.1e-25 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 103 - 205 1e-09 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 233 - 348 5.1e-16 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 560, 744
AccB1I GGYRCC 1 cut(s) 245
AccII CGCG 1 cut(s) 902
AciI CCGC 1 cut(s) 249
AclWI GGATC 5 cut(s) 411, 632, 645, 878, 974
AcoI YGGCCR 1 cut(s) 511
AcsI RAATTY 2 cut(s) 229, 347
AcuI CTGAAG 2 cut(s) 192, 849
AfaI GTAC 3 cut(s) 491, 802, 860
AfiI CCNNNNNNNGG 3 cut(s) 211, 605, 995
AflII CTTAAG 1 cut(s) 31
AflIII ACRYGT 1 cut(s) 651
AgsI TTSAA 4 cut(s) 131, 461, 533, 851
AjnI CCWGG 3 cut(s) 592, 982, 994
AleI CACNNNNGTG 1 cut(s) 274
AluBI AGCT 2 cut(s) 376, 661
AluI AGCT 2 cut(s) 376, 661
Alw26I GTCTC 1 cut(s) 109
AlwI GGATC 5 cut(s) 411, 632, 645, 878, 974
AlwNI CAGNNNCTG 1 cut(s) 215
AoxI GGCC 3 cut(s) 511, 674, 986
ApeKI GCWGC 2 cut(s) 215, 612
ApoI RAATTY 2 cut(s) 229, 347
Asp700I GAANNNNTTC 1 cut(s) 205
AspLEI GCGC 1 cut(s) 904
AspS9I GGNCC 5 cut(s) 434, 596, 674, 824, 986
AsuHPI GGTGA 2 cut(s) 672, 728
AsuII TTCGAA 1 cut(s) 413
AvaII GGWCC 3 cut(s) 434, 596, 824
AxyI CCTNAGG 1 cut(s) 399
BalI TGGCCA 1 cut(s) 513
BamHI GGATCC 1 cut(s) 637
BanI GGYRCC 1 cut(s) 245
BanII GRGCYC 1 cut(s) 690
BbvI GCAGC 2 cut(s) 202, 624
BccI CCATC 4 cut(s) 58, 638, 754, 1028
BceAI ACGGC 1 cut(s) 794
BciT130I CCWGG 3 cut(s) 594, 984, 996
BcoDI GTCTC 1 cut(s) 109
BfmI CTRYAG 1 cut(s) 939
BfrI CTTAAG 1 cut(s) 31
BglI GCCNNNNNGGC 1 cut(s) 519
BisI GCNGC 2 cut(s) 216, 613
BlsI GCNGC 2 cut(s) 217, 614
Bme1390I CCNGG 3 cut(s) 594, 984, 996
Bme18I GGWCC 3 cut(s) 434, 596, 824
BmgT120I GGNCC 5 cut(s) 434, 596, 674, 824, 986
BmiI GGNNCC 4 cut(s) 48, 247, 591, 639
BmrFI CCNGG 3 cut(s) 594, 984, 996
BmrI ACTGGG 1 cut(s) 215
BmsI GCATC 3 cut(s) 185, 693, 1008
BmuI ACTGGG 1 cut(s) 215
BplI GAGNNNNNCTC 2 cut(s) 106, 138
BpmI CTGGAG 1 cut(s) 293
Bpu10I CCTNAGC 1 cut(s) 684
Bpu14I TTCGAA 1 cut(s) 413
Bsa29I ATCGAT 1 cut(s) 406
BsaJI CCNNGG 4 cut(s) 258, 549, 585, 983
BsaXI ACNNNNNCTCC 2 cut(s) 564, 594
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 605, 995
Bse1I ACTGG 3 cut(s) 221, 276, 508
Bse21I CCTNAGG 1 cut(s) 399
Bse3DI GCAATG 1 cut(s) 522
BseBI CCWGG 3 cut(s) 594, 984, 996
BseCI ATCGAT 1 cut(s) 406
BseDI CCNNGG 4 cut(s) 258, 549, 585, 983
BseGI GGATG 2 cut(s) 375, 765
BseLI CCNNNNNNNGG 3 cut(s) 211, 605, 995
BseMI GCAATG 1 cut(s) 522
BseMII CTCAG 3 cut(s) 171, 390, 675
BseNI ACTGG 3 cut(s) 221, 276, 508
BseRI GAGGAG 1 cut(s) 207
BseXI GCAGC 2 cut(s) 202, 624
Bsh1236I CGCG 1 cut(s) 902
BshFI GGCC 3 cut(s) 513, 676, 988
BshNI GGYRCC 1 cut(s) 245
BshVI ATCGAT 1 cut(s) 406
BslFI GGGAC 2 cut(s) 341, 514
BslI CCNNNNNNNGG 3 cut(s) 211, 605, 995
BsmAI GTCTC 1 cut(s) 109
BsmFI GGGAC 2 cut(s) 341, 514
BsmI GAATGC 2 cut(s) 306, 421
BsnI GGCC 3 cut(s) 513, 676, 988
Bsp119I TTCGAA 1 cut(s) 413
Bsp1286I GDGCHC 1 cut(s) 690
Bsp143I GATC 6 cut(s) 403, 637, 718, 870, 906, 979
BspACI CCGC 1 cut(s) 249
BspANI GGCC 3 cut(s) 513, 676, 988
BspCNI CTCAG 3 cut(s) 172, 391, 676
BspDI ATCGAT 1 cut(s) 406
BspFNI CGCG 1 cut(s) 902
BspLI GGNNCC 4 cut(s) 48, 247, 591, 639
BspMAI CTGCAG 1 cut(s) 943
BspPI GGATC 5 cut(s) 411, 632, 645, 878, 974
BspT104I TTCGAA 1 cut(s) 413
BspT107I GGYRCC 1 cut(s) 245
BspTI CTTAAG 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 522
BsrI ACTGG 3 cut(s) 221, 276, 508
BssECI CCNNGG 4 cut(s) 258, 549, 585, 983
BssMI GATC 6 cut(s) 403, 637, 718, 870, 906, 979
BssT1I CCWWGG 1 cut(s) 258
Bst2UI CCWGG 3 cut(s) 594, 984, 996
Bst4CI ACNGT 6 cut(s) 60, 162, 550, 728, 805, 824
Bst6I CTCTTC 1 cut(s) 100
BstAFI CTTAAG 1 cut(s) 31
BstBI TTCGAA 1 cut(s) 413
BstC8I GCNNGC 2 cut(s) 244, 1021
BstDEI CTNAG 5 cut(s) 180, 188, 399, 684, 956
BstDSI CCRYGG 1 cut(s) 549
BstENI CCTNNNNNAGG 2 cut(s) 603, 993
BstF5I GGATG 2 cut(s) 375, 765
BstFNI CGCG 1 cut(s) 902
BstHHI GCGC 1 cut(s) 904
BstKTI GATC 6 cut(s) 406, 640, 721, 873, 909, 982
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 6 cut(s) 403, 637, 718, 870, 906, 979
BstMWI GCNNNNNNNGC 5 cut(s) 224, 233, 248, 519, 908
BstNI CCWGG 3 cut(s) 594, 984, 996
BstSCI CCNGG 3 cut(s) 592, 982, 994
BstSFI CTRYAG 1 cut(s) 939
BstUI CGCG 1 cut(s) 902
BstV1I GCAGC 2 cut(s) 202, 624
BstX2I RGATCY 1 cut(s) 637
BstXI CCANNNNNNTGG 2 cut(s) 454, 550
BstYI RGATCY 1 cut(s) 637
Bsu15I ATCGAT 1 cut(s) 406
Bsu36I CCTNAGG 1 cut(s) 399
BsuRI GGCC 3 cut(s) 513, 676, 988
BsuTUI ATCGAT 1 cut(s) 406
BtgI CCRYGG 1 cut(s) 549
BtgZI GCGATG 1 cut(s) 36
BtsCI GGATG 2 cut(s) 375, 765
BtsI GCAGTG 1 cut(s) 849
BtsIMutI CAGTG 2 cut(s) 269, 849
Cac8I GCNNGC 2 cut(s) 244, 1021
CaiI CAGNNNCTG 1 cut(s) 215
CfoI GCGC 1 cut(s) 904
Cfr13I GGNCC 5 cut(s) 434, 596, 674, 824, 986
ClaI ATCGAT 1 cut(s) 406
CsiI ACCWGGT 1 cut(s) 592
Csp6I GTAC 3 cut(s) 490, 801, 859
CspCI CAANNNNNGTGG 2 cut(s) 940, 975
CviAII CATG 1 cut(s) 308
CviQI GTAC 3 cut(s) 490, 801, 859
DdeI CTNAG 5 cut(s) 180, 188, 399, 684, 956
DpnI GATC 6 cut(s) 405, 639, 720, 872, 908, 981
DpnII GATC 6 cut(s) 403, 637, 718, 870, 906, 979
EaeI YGGCCR 1 cut(s) 511
Eam1104I CTCTTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 258
Eco24I GRGCYC 1 cut(s) 690
Eco47I GGWCC 3 cut(s) 434, 596, 824
Eco57I CTGAAG 2 cut(s) 192, 849
Eco81I CCTNAGG 1 cut(s) 399
EcoNI CCTNNNNNAGG 2 cut(s) 603, 993
EcoO109I RGGNCCY 1 cut(s) 674
EcoRII CCWGG 3 cut(s) 592, 982, 994
EcoT14I CCWWGG 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 690
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 1 cut(s) 311
FalI AAGNNNNNCTT 2 cut(s) 690, 722
FaqI GGGAC 2 cut(s) 341, 514
FatI CATG 1 cut(s) 307
Fnu4HI GCNGC 2 cut(s) 216, 613
FokI GGATG 2 cut(s) 382, 772
FriOI GRGCYC 1 cut(s) 690
Fsp4HI GCNGC 2 cut(s) 216, 613
GlaI GCGC 1 cut(s) 903
GluI GCNGC 2 cut(s) 216, 613
GsuI CTGGAG 1 cut(s) 293
HaeIII GGCC 3 cut(s) 513, 676, 988
HhaI GCGC 1 cut(s) 904
Hin1II CATG 1 cut(s) 311
Hin6I GCGC 1 cut(s) 902
HinP1I GCGC 1 cut(s) 902
HinfI GANTC 3 cut(s) 25, 117, 443
HphI GGTGA 2 cut(s) 672, 728
Hpy166II GTNNAC 3 cut(s) 484, 801, 931
Hpy188I TCNGA 5 cut(s) 24, 114, 181, 211, 1074
Hpy188III TCNNGA 5 cut(s) 641, 722, 788, 827, 977
Hpy8I GTNNAC 3 cut(s) 484, 801, 931
HpyAV CCTTC 5 cut(s) 216, 687, 700, 742, 1069
HpyCH4III ACNGT 6 cut(s) 60, 162, 550, 728, 805, 824
HpyCH4IV ACGT 3 cut(s) 388, 653, 857
HpyCH4V TGCA 4 cut(s) 170, 269, 527, 941
HpyF10VI GCNNNNNNNGC 5 cut(s) 224, 233, 248, 519, 908
HpyF3I CTNAG 5 cut(s) 180, 188, 399, 684, 956
HpySE526I ACGT 3 cut(s) 388, 653, 857
Hsp92II CATG 1 cut(s) 311
HspAI GCGC 1 cut(s) 902
Kzo9I GATC 6 cut(s) 403, 637, 718, 870, 906, 979
LmnI GCTCC 1 cut(s) 52
Lsp1109I GCAGC 2 cut(s) 202, 624
LweI GCATC 3 cut(s) 185, 693, 1008
MabI ACCWGGT 1 cut(s) 592
MaeII ACGT 3 cut(s) 388, 653, 857
MaeIII GTNAC 4 cut(s) 389, 538, 580, 896
MalI GATC 6 cut(s) 405, 639, 720, 872, 908, 981
MboI GATC 6 cut(s) 403, 637, 718, 870, 906, 979
MboII GAAGA 6 cut(s) 87, 309, 452, 545, 842, 979
MflI RGATCY 1 cut(s) 637
MhlI GDGCHC 1 cut(s) 690
MlsI TGGCCA 1 cut(s) 513
MluCI AATT 5 cut(s) 229, 347, 448, 472, 1079
MluNI TGGCCA 1 cut(s) 513
MlyI GAGTC 1 cut(s) 111
Mox20I TGGCCA 1 cut(s) 513
MroXI GAANNNNTTC 1 cut(s) 205
MscI TGGCCA 1 cut(s) 513
MseI TTAA 3 cut(s) 32, 380, 735
MslI CAYNNNNRTG 1 cut(s) 274
Msp20I TGGCCA 1 cut(s) 513
MspCI CTTAAG 1 cut(s) 31
MspR9I CCNGG 3 cut(s) 594, 984, 996
Mva1269I GAATGC 2 cut(s) 306, 421
MvaI CCWGG 3 cut(s) 594, 984, 996
MvnI CGCG 1 cut(s) 902
MwoI GCNNNNNNNGC 5 cut(s) 224, 233, 248, 519, 908
NdeII GATC 6 cut(s) 403, 637, 718, 870, 906, 979
NlaIII CATG 1 cut(s) 311
NlaIV GGNNCC 4 cut(s) 48, 247, 591, 639
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 1 cut(s) 389
NspV TTCGAA 1 cut(s) 413
OliI CACNNNNGTG 1 cut(s) 274
PctI GAATGC 2 cut(s) 306, 421
PdmI GAANNNNTTC 1 cut(s) 205
PfeI GAWTC 2 cut(s) 25, 443
PkrI GCNGC 2 cut(s) 217, 614
PleI GAGTC 1 cut(s) 111
PpsI GAGTC 1 cut(s) 111
PsiI TTATAA 2 cut(s) 560, 744
Psp6I CCWGG 3 cut(s) 592, 982, 994
PspGI CCWGG 3 cut(s) 592, 982, 994
PspN4I GGNNCC 4 cut(s) 48, 247, 591, 639
PspPI GGNCC 5 cut(s) 434, 596, 674, 824, 986
PstI CTGCAG 1 cut(s) 943
PstNI CAGNNNCTG 1 cut(s) 215
PsuI RGATCY 1 cut(s) 637
RsaI GTAC 3 cut(s) 491, 802, 860
RsaNI GTAC 3 cut(s) 490, 801, 859
RseI CAYNNNNRTG 1 cut(s) 274
SaqAI TTAA 3 cut(s) 32, 380, 735
SatI GCNGC 2 cut(s) 216, 613
Sau3AI GATC 6 cut(s) 403, 637, 718, 870, 906, 979
Sau96I GGNCC 5 cut(s) 434, 596, 674, 824, 986
SchI GAGTC 1 cut(s) 111
ScrFI CCNGG 3 cut(s) 594, 984, 996
SduI GDGCHC 1 cut(s) 690
SexAI ACCWGGT 1 cut(s) 592
SfaNI GCATC 3 cut(s) 185, 693, 1008
SfcI CTRYAG 1 cut(s) 939
SfuI TTCGAA 1 cut(s) 413
SinI GGWCC 3 cut(s) 434, 596, 824
SmiMI CAYNNNNRTG 1 cut(s) 274
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
Sse9I AATT 5 cut(s) 229, 347, 448, 472, 1079
SsiI CCGC 1 cut(s) 249
StyD4I CCNGG 3 cut(s) 592, 982, 994
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 6 cut(s) 60, 162, 550, 728, 805, 824
TaiI ACGT 3 cut(s) 391, 656, 860
TaqI TCGA 3 cut(s) 406, 413, 1026
TaqII GACCGA 1 cut(s) 1036
TasI AATT 5 cut(s) 229, 347, 448, 472, 1079
TatI WGTACW 2 cut(s) 489, 800
TfiI GAWTC 2 cut(s) 25, 443
Tru1I TTAA 3 cut(s) 32, 380, 735
Tru9I TTAA 3 cut(s) 32, 380, 735
TscAI CASTG 2 cut(s) 276, 849
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 2 cut(s) 215, 612
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 2 cut(s) 296, 386
TspGWI ACGGA 3 cut(s) 52, 492, 683
TspRI CASTG 2 cut(s) 276, 849
Vha464I CTTAAG 1 cut(s) 31
VpaK11BI GGWCC 3 cut(s) 434, 596, 824
XagI CCTNNNNNAGG 2 cut(s) 603, 993
XapI RAATTY 2 cut(s) 229, 347
XmnI GAANNNNTTC 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.