RLG00000010826

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0004018
Physical Location & Seq
Forward (+)
4620627 .. 4623571
2945 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010826

Sequence Viewer

Length: 1098 bp
ATGGCGTCTTTATCGGTCACTCTCAATCTCTTAAGGCCTTGTTCCATTGCTCCTAAGCCATCTCCGTCTTTGAGATTCTGTGATGTCCGAAACTCTGCTTCTTCCTATTGTTCTACTTCTCGACGACTCGCTCTCTTTCACCTTGGCTCTGCTCTTCCTCAGTCACAAATATTAGGTGCAAAAGCATCTCAATTGCTGAGAGGAGAGGGGAAACCTGTAGAGGCTGCCCCCATTGCTGATTTGGCACAAGCAGGTGCTGCAATTGCTCAGGAAGATGCATTGGAATGGGTAAAAAATGATAAACGAAGAATGCTTCATGTTGTCTATCGTGTTGGGGACTTGGACAAGACTATAAAATTCTACACTGAGTGTTTGGGGATGAAGCTGTTGAGAAAACGTGACATACCCGAGGAGCAATATTCGAATGCTTTTCTTGGATACGGGCCAGAAGATTCCAACTTTACTGTTGAACTTACATATAATTATGGAGTGGACAAGTATGACATTGGGACTGGTTTTGGCCACTTTGGTATTGCAGTTGAAGACGTTGCCAAAAAAGTGGAACTTATAAAGGCAAAGGGAGGGAAAGTTACCAGGGAACCTGGTCCTGCCAATGGTGGCAGTACAATAATTGCTCTTATTGAGGATCCTGATGGTTATCCATTTGAGCTTTTAGAGAAATGGCCTACACCAGAGCCCTTTTCACAAGTGATGCTTCGTGTAGGTGATCTTGACCGTGCTATTAACTTTTATACGAAGGCTTTTGGAATGGAACTTCTCCGCACAAGAGATAACCCCGAATACAAGTATACAGTAGCCATGTTGGGATATGGTACTGAAGATAATAGTCCAGTGCTTGAACTGACATATAACTATGGGATCACTGATTATGATAAAGGAAATGGTTATGCTCAGATTGCAATAGGCACAGACGATGTTTATAAAAGTGCAGAAGCAGTTAAACTCTCTGGAGGGAAGATTATTCTTGAACCTGGTCCCTTGCCAGGTATCAACACCAAGATAACTGCTTGCTTAGACCCCGATGGCTGGAAATCGGTTTTTGTTGATAATATTGATTTTCTGAAGGAATTAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

366

Amino Acids

40.1

Weight (kDa)

5.21

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 104 - 224 1.2e-25 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 106 - 206 2e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 235 - 351 1.9e-18 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 569, 942
AarI CACCTGC 1 cut(s) 242
Acc36I ACCTGC 1 cut(s) 242
AccI GTMKAC 1 cut(s) 809
AciI CCGC 1 cut(s) 781
AclWI GGATC 3 cut(s) 641, 654, 887
AcoI YGGCCR 1 cut(s) 520
AcsI RAATTY 1 cut(s) 356
AcuI CTGAAG 1 cut(s) 858
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 369
AfaI GTAC 2 cut(s) 625, 835
AfiI CCNNNNNNNGG 3 cut(s) 614, 1004, 1047
AflII CTTAAG 1 cut(s) 31
AgsI TTSAA 4 cut(s) 470, 542, 860, 989
AjnI CCWGG 4 cut(s) 593, 601, 991, 1003
AluBI AGCT 2 cut(s) 385, 670
AluI AGCT 2 cut(s) 385, 670
AlwI GGATC 3 cut(s) 641, 654, 887
AlwNI CAGNNNCTG 1 cut(s) 257
Ama87I CYCGRG 1 cut(s) 407
AoxI GGCC 4 cut(s) 35, 443, 520, 683
ApeKI GCWGC 2 cut(s) 224, 257
ApoI RAATTY 1 cut(s) 356
AspS9I GGNCC 3 cut(s) 443, 605, 995
AsuHPI GGTGA 2 cut(s) 131, 737
AsuII TTCGAA 1 cut(s) 422
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 2 cut(s) 605, 995
BalI TGGCCA 1 cut(s) 522
BamHI GGATCC 1 cut(s) 646
BanII GRGCYC 1 cut(s) 699
BbsI GAAGAC 1 cut(s) 549
BbvI GCAGC 2 cut(s) 211, 244
BccI CCATC 3 cut(s) 67, 647, 1037
BciT130I CCWGG 4 cut(s) 595, 603, 993, 1005
BciVI GTATCC 1 cut(s) 431
BfmI CTRYAG 1 cut(s) 216
BfrI CTTAAG 1 cut(s) 31
BfuAI ACCTGC 1 cut(s) 242
BfuI GTATCC 1 cut(s) 431
BisI GCNGC 2 cut(s) 225, 258
BlsI GCNGC 2 cut(s) 226, 259
Bme1390I CCNGG 4 cut(s) 595, 603, 993, 1005
Bme18I GGWCC 2 cut(s) 605, 995
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 3 cut(s) 443, 605, 995
BmiI GGNNCC 3 cut(s) 600, 648, 997
BmrFI CCNGG 4 cut(s) 595, 603, 993, 1005
BmsI GCATC 3 cut(s) 194, 265, 702
BpiI GAAGAC 1 cut(s) 549
BpmI CTGGAG 1 cut(s) 990
Bpu10I CCTNAGC 2 cut(s) 54, 267
Bpu14I TTCGAA 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 657
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 142, 408, 594
BsaXI ACNNNNNCTCC 2 cut(s) 573, 603
Bsc4I CCNNNNNNNGG 3 cut(s) 614, 1004, 1047
Bse1I ACTGG 2 cut(s) 517, 851
Bse3DI GCAATG 2 cut(s) 45, 231
Bse8I GATNNNNATC 1 cut(s) 657
BseBI CCWGG 4 cut(s) 595, 603, 993, 1005
BseDI CCNNGG 3 cut(s) 142, 408, 594
BseGI GGATG 1 cut(s) 384
BseJI GATNNNNATC 1 cut(s) 657
BseLI CCNNNNNNNGG 3 cut(s) 614, 1004, 1047
BseMI GCAATG 2 cut(s) 45, 231
BseMII CTCAG 5 cut(s) 173, 188, 281, 357, 926
BseNI ACTGG 2 cut(s) 517, 851
BseRI GAGGAG 2 cut(s) 216, 425
BseXI GCAGC 2 cut(s) 211, 244
BsgI GTGCAG 1 cut(s) 969
BshFI GGCC 4 cut(s) 37, 445, 522, 685
BsiHKCI CYCGRG 1 cut(s) 407
BslFI GGGAC 3 cut(s) 350, 523, 981
BslI CCNNNNNNNGG 3 cut(s) 614, 1004, 1047
BsmFI GGGAC 3 cut(s) 350, 523, 981
BsmI GAATGC 2 cut(s) 315, 430
BsnI GGCC 4 cut(s) 37, 445, 522, 685
BsoBI CYCGRG 1 cut(s) 407
Bsp119I TTCGAA 1 cut(s) 422
Bsp1286I GDGCHC 1 cut(s) 699
Bsp143I GATC 3 cut(s) 646, 727, 879
BspACI CCGC 1 cut(s) 781
BspANI GGCC 4 cut(s) 37, 445, 522, 685
BspCNI CTCAG 5 cut(s) 172, 189, 280, 358, 925
BspLI GGNNCC 3 cut(s) 600, 648, 997
BspMI ACCTGC 1 cut(s) 242
BspPI GGATC 3 cut(s) 641, 654, 887
BspQI GCTCTTC 1 cut(s) 159
BspT104I TTCGAA 1 cut(s) 422
BspTI CTTAAG 1 cut(s) 31
BsrDI GCAATG 2 cut(s) 45, 231
BsrI ACTGG 2 cut(s) 517, 851
BssECI CCNNGG 3 cut(s) 142, 408, 594
BssMI GATC 3 cut(s) 646, 727, 879
BssNAI GTATAC 1 cut(s) 810
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 142
Bst1107I GTATAC 1 cut(s) 810
Bst2UI CCWGG 4 cut(s) 595, 603, 993, 1005
Bst4CI ACNGT 3 cut(s) 466, 737, 814
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 31
BstAPI GCANNNNNTGC 1 cut(s) 257
BstBI TTCGAA 1 cut(s) 422
BstC8I GCNNGC 1 cut(s) 1030
BstDEI CTNAG 7 cut(s) 54, 159, 197, 267, 366, 912, 1033
BstF5I GGATG 1 cut(s) 384
BstKTI GATC 3 cut(s) 649, 730, 882
BstMBI GATC 3 cut(s) 646, 727, 879
BstMWI GCNNNNNNNGC 5 cut(s) 233, 242, 257, 263, 917
BstNI CCWGG 4 cut(s) 595, 603, 993, 1005
BstSCI CCNGG 4 cut(s) 593, 601, 991, 1003
BstSFI CTRYAG 1 cut(s) 216
BstV1I GCAGC 2 cut(s) 211, 244
BstV2I GAAGAC 1 cut(s) 549
BstX2I RGATCY 1 cut(s) 646
BstXI CCANNNNNNTGG 1 cut(s) 559
BstYI RGATCY 1 cut(s) 646
BstZ17I GTATAC 1 cut(s) 810
BsuI GTATCC 1 cut(s) 431
BsuRI GGCC 4 cut(s) 37, 445, 522, 685
BtsCI GGATG 1 cut(s) 384
BtsIMutI CAGTG 3 cut(s) 363, 858, 882
BveI ACCTGC 1 cut(s) 242
Cac8I GCNNGC 1 cut(s) 1030
CaiI CAGNNNCTG 1 cut(s) 257
Cfr13I GGNCC 3 cut(s) 443, 605, 995
CsiI ACCWGGT 2 cut(s) 601, 991
Csp6I GTAC 2 cut(s) 624, 834
CviAII CATG 2 cut(s) 317, 820
CviQI GTAC 2 cut(s) 624, 834
DdeI CTNAG 7 cut(s) 54, 159, 197, 267, 366, 912, 1033
DpnI GATC 3 cut(s) 648, 729, 881
DpnII GATC 3 cut(s) 646, 727, 879
DraIII CACNNNGTG 1 cut(s) 369
EaeI YGGCCR 1 cut(s) 520
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 142
Eco147I AGGCCT 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 699
Eco47I GGWCC 2 cut(s) 605, 995
Eco57I CTGAAG 1 cut(s) 858
Eco88I CYCGRG 1 cut(s) 407
EcoRII CCWGG 4 cut(s) 593, 601, 991, 1003
EcoT14I CCWWGG 1 cut(s) 142
EcoT22I ATGCAT 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 699
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 2 cut(s) 320, 823
FalI AAGNNNNNCTT 4 cut(s) 549, 581, 699, 731
FaqI GGGAC 3 cut(s) 350, 523, 981
FatI CATG 2 cut(s) 316, 819
FblI GTMKAC 1 cut(s) 809
Fnu4HI GCNGC 2 cut(s) 225, 258
FokI GGATG 1 cut(s) 391
FriOI GRGCYC 1 cut(s) 699
Fsp4HI GCNGC 2 cut(s) 225, 258
GluI GCNGC 2 cut(s) 225, 258
GsuI CTGGAG 1 cut(s) 990
HaeIII GGCC 4 cut(s) 37, 445, 522, 685
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 320, 823
HinfI GANTC 3 cut(s) 75, 126, 452
HphI GGTGA 2 cut(s) 131, 737
Hpy166II GTNNAC 2 cut(s) 493, 810
Hpy188I TCNGA 3 cut(s) 89, 915, 1083
Hpy188III TCNNGA 6 cut(s) 120, 269, 650, 731, 969, 986
Hpy8I GTNNAC 2 cut(s) 493, 810
Hpy99I CGWCG 1 cut(s) 126
HpyAV CCTTC 2 cut(s) 751, 1078
HpyCH4III ACNGT 3 cut(s) 466, 737, 814
HpyCH4IV ACGT 2 cut(s) 397, 546
HpyCH4V TGCA 6 cut(s) 179, 260, 278, 536, 920, 950
HpyF10VI GCNNNNNNNGC 5 cut(s) 233, 242, 257, 263, 917
HpyF3I CTNAG 7 cut(s) 54, 159, 197, 267, 366, 912, 1033
HpySE526I ACGT 2 cut(s) 397, 546
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 320, 823
Kzo9I GATC 3 cut(s) 646, 727, 879
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 2 cut(s) 55, 412
Lsp1109I GCAGC 2 cut(s) 211, 244
LweI GCATC 3 cut(s) 194, 265, 702
MabI ACCWGGT 2 cut(s) 601, 991
MaeII ACGT 2 cut(s) 397, 546
MaeIII GTNAC 4 cut(s) 16, 162, 398, 589
MalI GATC 3 cut(s) 648, 729, 881
MboI GATC 3 cut(s) 646, 727, 879
MboII GAAGA 8 cut(s) 93, 146, 284, 318, 461, 554, 851, 988
MfeI CAATTG 2 cut(s) 191, 261
MflI RGATCY 1 cut(s) 646
MhlI GDGCHC 1 cut(s) 699
MlsI TGGCCA 1 cut(s) 522
MluCI AATT 6 cut(s) 191, 261, 356, 481, 630, 1088
MluNI TGGCCA 1 cut(s) 522
MlyI GAGTC 1 cut(s) 120
MmeI TCCRAC 1 cut(s) 480
MnlI CCTC 8 cut(s) 168, 194, 199, 214, 403, 575, 637, 965
Mox20I TGGCCA 1 cut(s) 522
Mph1103I ATGCAT 1 cut(s) 280
MscI TGGCCA 1 cut(s) 522
MseI TTAA 3 cut(s) 32, 744, 960
MslI CAYNNNNRTG 1 cut(s) 283
Msp20I TGGCCA 1 cut(s) 522
MspCI CTTAAG 1 cut(s) 31
MspR9I CCNGG 4 cut(s) 595, 603, 993, 1005
MunI CAATTG 2 cut(s) 191, 261
Mva1269I GAATGC 2 cut(s) 315, 430
MvaI CCWGG 4 cut(s) 595, 603, 993, 1005
MwoI GCNNNNNNNGC 5 cut(s) 233, 242, 257, 263, 917
NdeII GATC 3 cut(s) 646, 727, 879
NlaIII CATG 2 cut(s) 320, 823
NlaIV GGNNCC 3 cut(s) 600, 648, 997
NmuCI GTSAC 3 cut(s) 16, 162, 398
NsiI ATGCAT 1 cut(s) 280
NspV TTCGAA 1 cut(s) 422
PaqCI CACCTGC 1 cut(s) 242
PceI AGGCCT 1 cut(s) 37
PciSI GCTCTTC 1 cut(s) 159
PctI GAATGC 2 cut(s) 315, 430
PfeI GAWTC 2 cut(s) 75, 452
PkrI GCNGC 2 cut(s) 226, 259
PleI GAGTC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 120
PsiI TTATAA 2 cut(s) 569, 942
Psp6I CCWGG 4 cut(s) 593, 601, 991, 1003
PspGI CCWGG 4 cut(s) 593, 601, 991, 1003
PspN4I GGNNCC 3 cut(s) 600, 648, 997
PspPI GGNCC 3 cut(s) 443, 605, 995
PstNI CAGNNNCTG 1 cut(s) 257
PsuI RGATCY 1 cut(s) 646
RsaI GTAC 2 cut(s) 625, 835
RsaNI GTAC 2 cut(s) 624, 834
RseI CAYNNNNRTG 1 cut(s) 283
SapI GCTCTTC 1 cut(s) 159
SaqAI TTAA 3 cut(s) 32, 744, 960
SatI GCNGC 2 cut(s) 225, 258
Sau3AI GATC 3 cut(s) 646, 727, 879
Sau96I GGNCC 3 cut(s) 443, 605, 995
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 4 cut(s) 595, 603, 993, 1005
SduI GDGCHC 1 cut(s) 699
SexAI ACCWGGT 2 cut(s) 601, 991
SfaNI GCATC 3 cut(s) 194, 265, 702
SfcI CTRYAG 1 cut(s) 216
SfuI TTCGAA 1 cut(s) 422
SinI GGWCC 2 cut(s) 605, 995
SmiMI CAYNNNNRTG 1 cut(s) 283
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
Sse9I AATT 6 cut(s) 191, 261, 356, 481, 630, 1088
SseBI AGGCCT 1 cut(s) 37
SsiI CCGC 1 cut(s) 781
SspI AATATT 3 cut(s) 171, 419, 1072
StuI AGGCCT 1 cut(s) 37
StyD4I CCNGG 4 cut(s) 593, 601, 991, 1003
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 3 cut(s) 466, 737, 814
TaiI ACGT 2 cut(s) 400, 549
TaqI TCGA 2 cut(s) 121, 422
TaqII GACCGA 1 cut(s) 4
TasI AATT 6 cut(s) 191, 261, 356, 481, 630, 1088
TatI WGTACW 1 cut(s) 623
TfiI GAWTC 2 cut(s) 75, 452
Tru1I TTAA 3 cut(s) 32, 744, 960
Tru9I TTAA 3 cut(s) 32, 744, 960
TscAI CASTG 3 cut(s) 370, 858, 889
TseFI GTSAC 3 cut(s) 16, 162, 398
TseI GCWGC 2 cut(s) 224, 257
Tsp45I GTSAC 3 cut(s) 16, 162, 398
TspDTI ATGAA 2 cut(s) 305, 395
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 3 cut(s) 370, 858, 889
Vha464I CTTAAG 1 cut(s) 31
VpaK11BI GGWCC 2 cut(s) 605, 995
XapI RAATTY 1 cut(s) 356
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XmiI GTMKAC 1 cut(s) 809
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.