RLG00000016218

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0004018
Physical Location & Seq
Reverse (-)
4804673 .. 4807402
2730 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016218

Sequence Viewer

Length: 1092 bp
ATGGTGAGGATAATACCTATGGCCGCCTCCTCCATCCGACCCTCCCTCTCCACCTTCAGGTTCGCCGGAGCTTCCCGCCTCGGCGGCCTCTCCCTCTCCACCTTCGCTCCGCCCCGGAGGCAGCTCGCCTTCGTCCACCTCGGTAGCGCTGTGTCACAGTCGCAGTCGTTTGGTTTGAAAGCTTCGAGGATGTTGAGGAGTGATGGTAAAACCATGGGGGTGGTGTCTGCTGGTAATGCAGCACAGGCAAGCACAACATCGCCGGAGAATGTCCTGGAGTGGGTCAAGCAGGACAAACGGAGGCTTCTCCATGTTGTATACCGCGTTGGGGATTTGGACAGGACCATAAAGTTCTACACGGAGTGCCTGGGAATGAAATTGCTGCGGAAACGTGACATACCAGAGGAAAGATATACAAATGCTTTTCTTGGATATGGGCCGGAGGATTCCCATTTTGTCATCGAACTCACTTACAATTATGGGGTTGACAAGTATGATATTGGAACTGCATTTGGTCATTTTGGTGTTGCTGTTGAGGATGTTGCTAAGACTGTGGAACTTGTGAAAGCTAAGGGAGGCAAGGTAACCCGAGAACCTGGTCCAGTGAAAGGTGGAAATACAGTAATTGCTTTTGTTGAAGATCCTGATGGTTATAAGTTTGAACTCTTGGAAAGAGGGCCTACACCTGAGCCCTTGTGTCAAGTTATGCTTCGTGTAGGTGATCTTGATCGTGCCATAGCTTTTTATGAGAAGGCTTTTGGCATGGAGCTTCTTCGCAAGAGAGATAATCCAGAGTACAAGTATACTATAGCAATGCTCGGTTATGGCCCTGAAGACAAAAACGCTGTTCTGGAATTGACATTCAACTACGGGGTCACTGAATACGACAAAGGAAATGCTTATGCTCAGATAGCGGTAGGCACGGATGATGTCTATAAAACTGCTGAAGCAATTAAACTCTGCGGAGGCAAGATTACGCGGGAACCTGGGCCATTACCTGGTCTTAACACAAAGATTACTGCTTGCTTGGACCCTGATGGTTGGAAGTCGGTTTTCGTGGATAATGTTGATTTCCTCAAGGAATTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

364

Amino Acids

40.04

Weight (kDa)

7.6

Isoelectric Point (pI)

34.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 101 - 222 4.9e-27 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 104 - 211 1.9e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 234 - 349 1.2e-18 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 654
AccB7I CCANNNNNTGG 1 cut(s) 998
AccI GTMKAC 2 cut(s) 318, 803
AccII CGCG 2 cut(s) 324, 979
AciI CCGC 9 cut(s) 24, 76, 84, 110, 322, 385, 914, 963, 979
AclWI GGATC 1 cut(s) 635
AcoI YGGCCR 1 cut(s) 21
AcuI CTGAAG 3 cut(s) 40, 852, 966
AdeI CACNNNGTG 1 cut(s) 363
AfaI GTAC 1 cut(s) 797
AfeI AGCGCT 1 cut(s) 148
AfiI CCNNNNNNNGG 5 cut(s) 57, 280, 328, 608, 998
AgsI TTSAA 4 cut(s) 178, 638, 662, 865
AjnI CCWGG 5 cut(s) 273, 366, 595, 985, 997
AluBI AGCT 6 cut(s) 71, 124, 182, 569, 740, 769
AluI AGCT 6 cut(s) 71, 124, 182, 569, 740, 769
AlwI GGATC 1 cut(s) 635
Ama87I CYCGRG 1 cut(s) 588
Aor51HI AGCGCT 1 cut(s) 148
AoxI GGCC 6 cut(s) 21, 85, 437, 677, 826, 989
ApeKI GCWGC 3 cut(s) 121, 239, 382
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 7 cut(s) 342, 437, 599, 677, 827, 989, 1030
AsuC2I CCSGG 1 cut(s) 115
AsuHPI GGTGA 2 cut(s) 16, 731
AvaI CYCGRG 1 cut(s) 588
AvaII GGWCC 3 cut(s) 342, 599, 1030
BanII GRGCYC 1 cut(s) 693
BbsI GAAGAC 1 cut(s) 840
BbvI GCAGC 3 cut(s) 133, 251, 369
BccI CCATC 4 cut(s) 41, 197, 641, 1031
BciT130I CCWGG 5 cut(s) 275, 368, 597, 987, 999
BcnI CCSGG 1 cut(s) 115
BfmI CTRYAG 1 cut(s) 807
BfoI RGCGCY 1 cut(s) 150
BglI GCCNNNNNGGC 2 cut(s) 84, 118
BisI GCNGC 5 cut(s) 24, 85, 122, 240, 383
BlsI GCNGC 5 cut(s) 25, 86, 123, 241, 384
Bme1390I CCNGG 6 cut(s) 115, 275, 368, 597, 987, 999
Bme18I GGWCC 3 cut(s) 342, 599, 1030
BmeT110I CYCGRG 1 cut(s) 588
BmgT120I GGNCC 7 cut(s) 342, 437, 599, 677, 827, 989, 1030
BmiI GGNNCC 2 cut(s) 984, 1032
BmrFI CCNGG 6 cut(s) 115, 275, 368, 597, 987, 999
BpiI GAAGAC 1 cut(s) 840
BpmI CTGGAG 1 cut(s) 296
Bpu10I CCTNAGC 2 cut(s) 570, 687
BpuEI CTTGAG 1 cut(s) 1061
BpuMI CCSGG 1 cut(s) 115
BsaBI GATNNNNATC 1 cut(s) 726
BsaJI CCNNGG 6 cut(s) 79, 113, 139, 213, 367, 986
BsaXI ACNNNNNCTCC 6 cut(s) 91, 121, 190, 220, 567, 597
Bsc4I CCNNNNNNNGG 5 cut(s) 57, 280, 328, 608, 998
Bse1I ACTGG 1 cut(s) 602
Bse3DI GCAATG 1 cut(s) 819
Bse8I GATNNNNATC 1 cut(s) 726
BseBI CCWGG 5 cut(s) 275, 368, 597, 987, 999
BseDI CCNNGG 6 cut(s) 79, 113, 139, 213, 367, 986
BseGI GGATG 4 cut(s) 33, 195, 544, 931
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 5 cut(s) 57, 280, 328, 608, 998
BseMI GCAATG 1 cut(s) 819
BseMII CTCAG 2 cut(s) 678, 920
BseNI ACTGG 1 cut(s) 602
BseRI GAGGAG 2 cut(s) 19, 211
BseXI GCAGC 3 cut(s) 133, 251, 369
Bsh1236I CGCG 2 cut(s) 324, 979
BshFI GGCC 6 cut(s) 23, 87, 439, 679, 828, 991
BsiHKCI CYCGRG 1 cut(s) 588
BsiSI CCGG 4 cut(s) 66, 115, 263, 440
BslI CCNNNNNNNGG 5 cut(s) 57, 280, 328, 608, 998
BsnI GGCC 6 cut(s) 23, 87, 439, 679, 828, 991
BsoBI CYCGRG 1 cut(s) 588
Bsp1286I GDGCHC 1 cut(s) 693
Bsp143I GATC 3 cut(s) 640, 721, 727
Bsp19I CCATGG 1 cut(s) 213
BspACI CCGC 9 cut(s) 24, 76, 84, 110, 322, 385, 914, 963, 979
BspANI GGCC 6 cut(s) 23, 87, 439, 679, 828, 991
BspCNI CTCAG 2 cut(s) 679, 919
BspFNI CGCG 2 cut(s) 324, 979
BspLI GGNNCC 2 cut(s) 984, 1032
BspPI GGATC 1 cut(s) 635
BsrDI GCAATG 1 cut(s) 819
BsrI ACTGG 1 cut(s) 602
BssECI CCNNGG 6 cut(s) 79, 113, 139, 213, 367, 986
BssMI GATC 3 cut(s) 640, 721, 727
BssNAI GTATAC 2 cut(s) 319, 804
BssT1I CCWWGG 1 cut(s) 213
Bst1107I GTATAC 2 cut(s) 319, 804
Bst2UI CCWGG 5 cut(s) 275, 368, 597, 987, 999
Bst4CI ACNGT 3 cut(s) 159, 553, 622
BstC8I GCNNGC 3 cut(s) 126, 250, 1024
BstDEI CTNAG 4 cut(s) 546, 570, 687, 906
BstDSI CCRYGG 1 cut(s) 213
BstEII GGTNACC 1 cut(s) 583
BstF5I GGATG 4 cut(s) 33, 195, 544, 931
BstFNI CGCG 2 cut(s) 324, 979
BstH2I RGCGCY 1 cut(s) 150
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 3 cut(s) 643, 724, 730
BstMBI GATC 3 cut(s) 640, 721, 727
BstMWI GCNNNNNNNGC 5 cut(s) 84, 118, 236, 245, 911
BstNI CCWGG 5 cut(s) 275, 368, 597, 987, 999
BstPI GGTNACC 1 cut(s) 583
BstSCI CCNGG 6 cut(s) 113, 273, 366, 595, 985, 997
BstSFI CTRYAG 1 cut(s) 807
BstUI CGCG 2 cut(s) 324, 979
BstV1I GCAGC 3 cut(s) 133, 251, 369
BstV2I GAAGAC 1 cut(s) 840
BstX2I RGATCY 1 cut(s) 640
BstXI CCANNNNNNTGG 1 cut(s) 220
BstYI RGATCY 1 cut(s) 640
BstZ17I GTATAC 2 cut(s) 319, 804
BsuRI GGCC 6 cut(s) 23, 87, 439, 679, 828, 991
BtgI CCRYGG 1 cut(s) 213
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 4 cut(s) 33, 195, 544, 931
BtsIMutI CAGTG 2 cut(s) 609, 876
Cac8I GCNNGC 3 cut(s) 126, 250, 1024
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 7 cut(s) 342, 437, 599, 677, 827, 989, 1030
CsiI ACCWGGT 2 cut(s) 595, 997
Csp6I GTAC 1 cut(s) 796
CviAII CATG 3 cut(s) 214, 311, 763
CviQI GTAC 1 cut(s) 796
DdeI CTNAG 4 cut(s) 546, 570, 687, 906
DpnI GATC 3 cut(s) 642, 723, 729
DpnII GATC 3 cut(s) 640, 721, 727
DraIII CACNNNGTG 1 cut(s) 363
EaeI YGGCCR 1 cut(s) 21
EciI GGCGGA 1 cut(s) 99
Eco130I CCWWGG 1 cut(s) 213
Eco24I GRGCYC 1 cut(s) 693
Eco47I GGWCC 3 cut(s) 342, 599, 1030
Eco47III AGCGCT 1 cut(s) 148
Eco57I CTGAAG 3 cut(s) 40, 852, 966
Eco88I CYCGRG 1 cut(s) 588
Eco91I GGTNACC 1 cut(s) 583
EcoO109I RGGNCCY 1 cut(s) 677
EcoO65I GGTNACC 1 cut(s) 583
EcoRII CCWGG 5 cut(s) 273, 366, 595, 985, 997
EcoT14I CCWWGG 1 cut(s) 213
EcoT38I GRGCYC 1 cut(s) 693
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 3 cut(s) 217, 314, 766
FalI AAGNNNNNCTT 2 cut(s) 693, 725
FatI CATG 3 cut(s) 213, 310, 762
FauI CCCGC 2 cut(s) 83, 972
FblI GTMKAC 2 cut(s) 318, 803
Fnu4HI GCNGC 5 cut(s) 24, 85, 122, 240, 383
FokI GGATG 4 cut(s) 20, 202, 551, 938
FriOI GRGCYC 1 cut(s) 693
Fsp4HI GCNGC 5 cut(s) 24, 85, 122, 240, 383
GlaI GCGC 1 cut(s) 148
GluI GCNGC 5 cut(s) 24, 85, 122, 240, 383
GsuI CTGGAG 1 cut(s) 296
HaeII RGCGCY 1 cut(s) 150
HaeIII GGCC 6 cut(s) 23, 87, 439, 679, 828, 991
HapII CCGG 4 cut(s) 66, 115, 263, 440
HhaI GCGC 1 cut(s) 149
Hin1II CATG 3 cut(s) 217, 314, 766
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HindIII AAGCTT 1 cut(s) 180
HinfI GANTC 1 cut(s) 446
HpaII CCGG 4 cut(s) 66, 115, 263, 440
HphI GGTGA 2 cut(s) 16, 731
Hpy166II GTNNAC 4 cut(s) 136, 319, 487, 804
Hpy188I TCNGA 2 cut(s) 38, 909
Hpy188III TCNNGA 4 cut(s) 644, 725, 791, 851
Hpy8I GTNNAC 4 cut(s) 136, 319, 487, 804
HpyAV CCTTC 4 cut(s) 64, 112, 139, 745
HpyCH4III ACNGT 3 cut(s) 159, 553, 622
HpyCH4IV ACGT 1 cut(s) 391
HpyCH4V TGCA 2 cut(s) 239, 509
HpyF10VI GCNNNNNNNGC 5 cut(s) 84, 118, 236, 245, 911
HpyF3I CTNAG 4 cut(s) 546, 570, 687, 906
HpySE526I ACGT 1 cut(s) 391
Hsp92II CATG 3 cut(s) 217, 314, 766
HspAI GCGC 1 cut(s) 147
Kzo9I GATC 3 cut(s) 640, 721, 727
LmnI GCTCC 3 cut(s) 68, 112, 766
Lsp1109I GCAGC 3 cut(s) 133, 251, 369
MabI ACCWGGT 2 cut(s) 595, 997
MaeII ACGT 1 cut(s) 391
MaeIII GTNAC 4 cut(s) 153, 392, 583, 874
MalI GATC 3 cut(s) 642, 723, 729
MboI GATC 3 cut(s) 640, 721, 727
MboII GAAGA 3 cut(s) 650, 764, 845
MflI RGATCY 1 cut(s) 640
MhlI GDGCHC 1 cut(s) 693
MluCI AATT 6 cut(s) 377, 475, 624, 854, 951, 1082
MmeI TCCRAC 2 cut(s) 61, 1022
MseI TTAA 2 cut(s) 954, 1005
MslI CAYNNNNRTG 2 cut(s) 218, 522
MspI CCGG 4 cut(s) 66, 115, 263, 440
MspR9I CCNGG 6 cut(s) 115, 275, 368, 597, 987, 999
MvaI CCWGG 5 cut(s) 275, 368, 597, 987, 999
MvnI CGCG 2 cut(s) 324, 979
MwoI GCNNNNNNNGC 5 cut(s) 84, 118, 236, 245, 911
NciI CCSGG 1 cut(s) 115
NcoI CCATGG 1 cut(s) 213
NdeII GATC 3 cut(s) 640, 721, 727
NlaIII CATG 3 cut(s) 217, 314, 766
NlaIV GGNNCC 2 cut(s) 984, 1032
NmeAIII GCCGAG 1 cut(s) 60
NmuCI GTSAC 3 cut(s) 153, 392, 874
PfeI GAWTC 1 cut(s) 446
PflMI CCANNNNNTGG 1 cut(s) 998
PfoI TCCNGGA 1 cut(s) 273
PkrI GCNGC 5 cut(s) 25, 86, 123, 241, 384
PsiI TTATAA 1 cut(s) 654
Psp6I CCWGG 5 cut(s) 273, 366, 595, 985, 997
PspEI GGTNACC 1 cut(s) 583
PspGI CCWGG 5 cut(s) 273, 366, 595, 985, 997
PspN4I GGNNCC 2 cut(s) 984, 1032
PspPI GGNCC 7 cut(s) 342, 437, 599, 677, 827, 989, 1030
PsuI RGATCY 1 cut(s) 640
RsaI GTAC 1 cut(s) 797
RsaNI GTAC 1 cut(s) 796
RseI CAYNNNNRTG 2 cut(s) 218, 522
SaqAI TTAA 2 cut(s) 954, 1005
SatI GCNGC 5 cut(s) 24, 85, 122, 240, 383
Sau3AI GATC 3 cut(s) 640, 721, 727
Sau96I GGNCC 7 cut(s) 342, 437, 599, 677, 827, 989, 1030
ScrFI CCNGG 6 cut(s) 115, 275, 368, 597, 987, 999
SduI GDGCHC 1 cut(s) 693
SexAI ACCWGGT 2 cut(s) 595, 997
SfcI CTRYAG 1 cut(s) 807
SinI GGWCC 3 cut(s) 342, 599, 1030
SmiMI CAYNNNNRTG 2 cut(s) 218, 522
SmlI CTYRAG 1 cut(s) 1076
SmoI CTYRAG 1 cut(s) 1076
Sse9I AATT 6 cut(s) 377, 475, 624, 854, 951, 1082
SsiI CCGC 9 cut(s) 24, 76, 84, 110, 322, 385, 914, 963, 979
StyD4I CCNGG 6 cut(s) 113, 273, 366, 595, 985, 997
StyI CCWWGG 1 cut(s) 213
TaaI ACNGT 3 cut(s) 159, 553, 622
TaiI ACGT 1 cut(s) 394
TaqI TCGA 2 cut(s) 185, 462
TasI AATT 6 cut(s) 377, 475, 624, 854, 951, 1082
TatI WGTACW 1 cut(s) 795
TauI GCSGC 2 cut(s) 26, 87
TfiI GAWTC 1 cut(s) 446
Tru1I TTAA 2 cut(s) 954, 1005
Tru9I TTAA 2 cut(s) 954, 1005
TscAI CASTG 2 cut(s) 609, 883
TseFI GTSAC 3 cut(s) 153, 392, 874
TseI GCWGC 3 cut(s) 121, 239, 382
Tsp45I GTSAC 3 cut(s) 153, 392, 874
TspDTI ATGAA 1 cut(s) 389
TspGWI ACGGA 3 cut(s) 313, 374, 938
TspRI CASTG 2 cut(s) 609, 883
Van91I CCANNNNNTGG 1 cut(s) 998
VpaK11BI GGWCC 3 cut(s) 342, 599, 1030
XmiI GTMKAC 2 cut(s) 318, 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.