Rh6BG441900

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0004018
Physical Location & Seq
Forward (+)
65972552 .. 65976315
3764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG441900.1

Sequence Viewer

Length: 1188 bp
ATGGCGTCTTTATCGGTCGCTCTCAATCTCTTAAGGCCTTGTTCCATTGCTCCTAAGCCATCTCCGTCTTTGAGATTCTGTGATGTCCGAAACTCTGCTTCTTCCTATTGTTCTACTTCTCGAAGACTCGCTCTCTTTCACCTTGGCTCTGCTCTTCCTCAGTCACAAATATTAGGTGCAAAAGCATCTCAATTGCTGAGAGGAGAGGGGAAACCTGTAGAGGCTGCCCCAATTGCTGATTTGGCACAAGCAGGTGCTGCAATTGCTCAGGAAGATGCATTGGAATGGGTAAAAAATGATAAACGAAGAATGCTTCATGTCGTCTATCGTGTTGGGGACTTGGACAAGACTATAAAGTACGCGTTTATTTATTTATTTATTTTTGCAGAAAATAGGTTTCAACAATGCAGTTCTAGAGATAGCTATGTATTATTATTCATATGCAGATTCTACACTGAGTGTTTGGGGATGAAGCTGTTGAGAAAACGTGACATACCCGAGGAGCAATATTCGAACGCTTTTCTTGGATATGGGCCTGAAGATTCCAACTTTACTGTTGAACTTACATATAATTATGGAGTGGACAAGTATGACATTGGGACTGGTTTTGGCCACTTTGGTATTGCAGTTGAAGATGTTGCCAAAACAGTGGAACTTATAAAGGCAAAGGGAGGGAAAGTTACCAGGGAACCTGGTCCTGCCAATGGTGGCAGTACAATAATTGCTCTTATTGAGGATCCTGATGGTTATCCGTTTGAGCTTTTAGAGAAATGGCCTACACCAGAGCCCTTTTCACAAGTGATGCTTCGTGTAGGTGATCTTGACCGTGCTATTAACTTTTATATGAAGGCTTTTGGAATGGAACTTCTCCGCACAAGAGATAATCCCGAATACAAGTATACAGTAGCCATGTTGGGATATGGTACTGAAGATAATAGTCCAGTGCTTGAACTGACATATAACTATGGGATCACTGATTATGATAAAGGAAATGGTTATGCTCAGATTGCAATAGGCACAGACGATGTTTATAAAAGTGCAGAAGCAGTTAGACTCTCTGGAGGGAAGATTATTCTTGAACCTGGTCCCTTGCCAGGTATCAACACCAAGATAACTGCTTGCTTAGACCCCGATGGCTGGAAATCGGTTTTTGTTGATAATATTGATTTTCTGAAGGAATTAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

395

Amino Acids

43.8

Weight (kDa)

5.23

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 148 - 254 2.7e-19 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 149 - 236 1e-05 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 265 - 381 1.3e-17 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 659, 1032
AarI CACCTGC 1 cut(s) 242
Acc36I ACCTGC 1 cut(s) 242
AccI GTMKAC 1 cut(s) 899
AccII CGCG 1 cut(s) 362
AciI CCGC 1 cut(s) 871
AclWI GGATC 3 cut(s) 731, 744, 977
AcoI YGGCCR 1 cut(s) 610
AcuI CTGAAG 2 cut(s) 558, 948
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 459
AfaI GTAC 3 cut(s) 359, 715, 925
AfiI CCNNNNNNNGG 3 cut(s) 704, 1094, 1137
AflII CTTAAG 1 cut(s) 31
AflIII ACRYGT 1 cut(s) 360
AgsI TTSAA 5 cut(s) 401, 560, 632, 950, 1079
AjnI CCWGG 4 cut(s) 683, 691, 1081, 1093
AluBI AGCT 3 cut(s) 423, 475, 760
AluI AGCT 3 cut(s) 423, 475, 760
AlwI GGATC 3 cut(s) 731, 744, 977
AlwNI CAGNNNCTG 1 cut(s) 257
Ama87I CYCGRG 1 cut(s) 497
AoxI GGCC 4 cut(s) 35, 533, 610, 773
ApeKI GCWGC 2 cut(s) 224, 257
AspS9I GGNCC 3 cut(s) 533, 695, 1085
AsuHPI GGTGA 2 cut(s) 131, 827
AsuII TTCGAA 1 cut(s) 512
AvaI CYCGRG 1 cut(s) 497
AvaII GGWCC 2 cut(s) 695, 1085
BalI TGGCCA 1 cut(s) 612
BamHI GGATCC 1 cut(s) 736
BanII GRGCYC 1 cut(s) 789
BbsI GAAGAC 1 cut(s) 130
BbvI GCAGC 2 cut(s) 211, 244
BccI CCATC 3 cut(s) 67, 737, 1127
BciT130I CCWGG 4 cut(s) 685, 693, 1083, 1095
BfaI CTAG 1 cut(s) 414
BfmI CTRYAG 1 cut(s) 216
BfrI CTTAAG 1 cut(s) 31
BfuAI ACCTGC 1 cut(s) 242
BisI GCNGC 2 cut(s) 225, 258
BlsI GCNGC 2 cut(s) 226, 259
Bme1390I CCNGG 4 cut(s) 685, 693, 1083, 1095
Bme18I GGWCC 2 cut(s) 695, 1085
BmeT110I CYCGRG 1 cut(s) 497
BmgT120I GGNCC 3 cut(s) 533, 695, 1085
BmiI GGNNCC 3 cut(s) 690, 738, 1087
BmrFI CCNGG 4 cut(s) 685, 693, 1083, 1095
BmsI GCATC 3 cut(s) 194, 265, 792
BpiI GAAGAC 1 cut(s) 130
BpmI CTGGAG 1 cut(s) 1080
Bpu10I CCTNAGC 2 cut(s) 54, 267
Bpu14I TTCGAA 1 cut(s) 512
BsaBI GATNNNNATC 1 cut(s) 747
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 142, 498, 684
BsaXI ACNNNNNCTCC 2 cut(s) 663, 693
Bsc4I CCNNNNNNNGG 3 cut(s) 704, 1094, 1137
Bse1I ACTGG 2 cut(s) 607, 941
Bse3DI GCAATG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 747
BseBI CCWGG 4 cut(s) 685, 693, 1083, 1095
BseDI CCNNGG 3 cut(s) 142, 498, 684
BseGI GGATG 1 cut(s) 474
BseJI GATNNNNATC 1 cut(s) 747
BseLI CCNNNNNNNGG 3 cut(s) 704, 1094, 1137
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 5 cut(s) 173, 188, 281, 447, 1016
BseNI ACTGG 2 cut(s) 607, 941
BseRI GAGGAG 2 cut(s) 216, 515
BseXI GCAGC 2 cut(s) 211, 244
BsgI GTGCAG 1 cut(s) 1059
Bsh1236I CGCG 1 cut(s) 362
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 4 cut(s) 37, 535, 612, 775
BsiEI CGRYCG 1 cut(s) 18
BsiHKCI CYCGRG 1 cut(s) 497
BslFI GGGAC 3 cut(s) 350, 613, 1071
BslI CCNNNNNNNGG 3 cut(s) 704, 1094, 1137
BsmFI GGGAC 3 cut(s) 350, 613, 1071
BsmI GAATGC 1 cut(s) 315
BsnI GGCC 4 cut(s) 37, 535, 612, 775
BsoBI CYCGRG 1 cut(s) 497
Bsp119I TTCGAA 1 cut(s) 512
Bsp1286I GDGCHC 1 cut(s) 789
Bsp143I GATC 3 cut(s) 736, 817, 969
BspACI CCGC 1 cut(s) 871
BspANI GGCC 4 cut(s) 37, 535, 612, 775
BspCNI CTCAG 5 cut(s) 172, 189, 280, 448, 1015
BspFNI CGCG 1 cut(s) 362
BspLI GGNNCC 3 cut(s) 690, 738, 1087
BspMI ACCTGC 1 cut(s) 242
BspPI GGATC 3 cut(s) 731, 744, 977
BspQI GCTCTTC 1 cut(s) 159
BspT104I TTCGAA 1 cut(s) 512
BspTI CTTAAG 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 2 cut(s) 607, 941
BssECI CCNNGG 3 cut(s) 142, 498, 684
BssMI GATC 3 cut(s) 736, 817, 969
BssNAI GTATAC 1 cut(s) 900
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 142
Bst1107I GTATAC 1 cut(s) 900
Bst2UI CCWGG 4 cut(s) 685, 693, 1083, 1095
Bst4CI ACNGT 4 cut(s) 556, 649, 827, 904
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 31
BstAPI GCANNNNNTGC 1 cut(s) 257
BstBI TTCGAA 1 cut(s) 512
BstC8I GCNNGC 1 cut(s) 1120
BstDEI CTNAG 7 cut(s) 54, 159, 197, 267, 456, 1002, 1123
BstF5I GGATG 1 cut(s) 474
BstFNI CGCG 1 cut(s) 362
BstKTI GATC 3 cut(s) 739, 820, 972
BstMBI GATC 3 cut(s) 736, 817, 969
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 5 cut(s) 233, 242, 257, 263, 1007
BstNI CCWGG 4 cut(s) 685, 693, 1083, 1095
BstSCI CCNGG 4 cut(s) 683, 691, 1081, 1093
BstSFI CTRYAG 1 cut(s) 216
BstUI CGCG 1 cut(s) 362
BstV1I GCAGC 2 cut(s) 211, 244
BstV2I GAAGAC 1 cut(s) 130
BstX2I RGATCY 1 cut(s) 736
BstXI CCANNNNNNTGG 1 cut(s) 649
BstYI RGATCY 1 cut(s) 736
BstZ17I GTATAC 1 cut(s) 900
BsuRI GGCC 4 cut(s) 37, 535, 612, 775
BtsCI GGATG 1 cut(s) 474
BtsIMutI CAGTG 4 cut(s) 453, 654, 948, 972
BveI ACCTGC 1 cut(s) 242
Cac8I GCNNGC 1 cut(s) 1120
CaiI CAGNNNCTG 1 cut(s) 257
Cfr13I GGNCC 3 cut(s) 533, 695, 1085
CsiI ACCWGGT 2 cut(s) 691, 1081
Csp6I GTAC 3 cut(s) 358, 714, 924
CviAII CATG 2 cut(s) 317, 910
CviQI GTAC 3 cut(s) 358, 714, 924
DdeI CTNAG 7 cut(s) 54, 159, 197, 267, 456, 1002, 1123
DpnI GATC 3 cut(s) 738, 819, 971
DpnII GATC 3 cut(s) 736, 817, 969
DraIII CACNNNGTG 1 cut(s) 459
EaeI YGGCCR 1 cut(s) 610
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 142
Eco147I AGGCCT 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 789
Eco47I GGWCC 2 cut(s) 695, 1085
Eco57I CTGAAG 2 cut(s) 558, 948
Eco88I CYCGRG 1 cut(s) 497
EcoRII CCWGG 4 cut(s) 683, 691, 1081, 1093
EcoT14I CCWWGG 1 cut(s) 142
EcoT22I ATGCAT 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 789
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 2 cut(s) 320, 913
FalI AAGNNNNNCTT 2 cut(s) 789, 821
FaqI GGGAC 3 cut(s) 350, 613, 1071
FatI CATG 2 cut(s) 316, 909
FauNDI CATATG 1 cut(s) 440
FblI GTMKAC 1 cut(s) 899
Fnu4HI GCNGC 2 cut(s) 225, 258
FokI GGATG 1 cut(s) 481
FriOI GRGCYC 1 cut(s) 789
Fsp4HI GCNGC 2 cut(s) 225, 258
FspBI CTAG 1 cut(s) 414
GluI GCNGC 2 cut(s) 225, 258
GsuI CTGGAG 1 cut(s) 1080
HaeIII GGCC 4 cut(s) 37, 535, 612, 775
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 320, 913
HinfI GANTC 5 cut(s) 75, 126, 447, 542, 1053
HphI GGTGA 2 cut(s) 131, 827
Hpy166II GTNNAC 2 cut(s) 583, 900
Hpy188I TCNGA 3 cut(s) 89, 1005, 1173
Hpy188III TCNNGA 8 cut(s) 120, 269, 414, 740, 821, 887, 1059, 1076
Hpy8I GTNNAC 2 cut(s) 583, 900
HpyAV CCTTC 2 cut(s) 841, 1168
HpyCH4III ACNGT 4 cut(s) 556, 649, 827, 904
HpyCH4IV ACGT 1 cut(s) 487
HpyCH4V TGCA 9 cut(s) 179, 260, 278, 386, 408, 444, 626, 1010, 1040
HpyF10VI GCNNNNNNNGC 5 cut(s) 233, 242, 257, 263, 1007
HpyF3I CTNAG 7 cut(s) 54, 159, 197, 267, 456, 1002, 1123
HpySE526I ACGT 1 cut(s) 487
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 320, 913
Kzo9I GATC 3 cut(s) 736, 817, 969
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 2 cut(s) 55, 502
Lsp1109I GCAGC 2 cut(s) 211, 244
LweI GCATC 3 cut(s) 194, 265, 792
MabI ACCWGGT 2 cut(s) 691, 1081
MaeI CTAG 1 cut(s) 414
MaeII ACGT 1 cut(s) 487
MaeIII GTNAC 3 cut(s) 162, 488, 679
MalI GATC 3 cut(s) 738, 819, 971
MboI GATC 3 cut(s) 736, 817, 969
MboII GAAGA 9 cut(s) 93, 135, 146, 284, 318, 551, 644, 941, 1078
MfeI CAATTG 3 cut(s) 191, 231, 261
MflI RGATCY 1 cut(s) 736
MhlI GDGCHC 1 cut(s) 789
MlsI TGGCCA 1 cut(s) 612
MluCI AATT 6 cut(s) 191, 231, 261, 571, 720, 1178
MluI ACGCGT 1 cut(s) 360
MluNI TGGCCA 1 cut(s) 612
MlyI GAGTC 2 cut(s) 120, 1047
MmeI TCCRAC 1 cut(s) 570
MnlI CCTC 8 cut(s) 168, 194, 199, 214, 493, 665, 727, 1055
Mox20I TGGCCA 1 cut(s) 612
Mph1103I ATGCAT 1 cut(s) 280
MscI TGGCCA 1 cut(s) 612
MseI TTAA 2 cut(s) 32, 834
MslI CAYNNNNRTG 1 cut(s) 283
Msp20I TGGCCA 1 cut(s) 612
MspCI CTTAAG 1 cut(s) 31
MspR9I CCNGG 4 cut(s) 685, 693, 1083, 1095
MunI CAATTG 3 cut(s) 191, 231, 261
Mva1269I GAATGC 1 cut(s) 315
MvaI CCWGG 4 cut(s) 685, 693, 1083, 1095
MvnI CGCG 1 cut(s) 362
MwoI GCNNNNNNNGC 5 cut(s) 233, 242, 257, 263, 1007
NdeI CATATG 1 cut(s) 440
NdeII GATC 3 cut(s) 736, 817, 969
NlaIII CATG 2 cut(s) 320, 913
NlaIV GGNNCC 3 cut(s) 690, 738, 1087
NmuCI GTSAC 2 cut(s) 162, 488
NsiI ATGCAT 1 cut(s) 280
NspV TTCGAA 1 cut(s) 512
PaqCI CACCTGC 1 cut(s) 242
PceI AGGCCT 1 cut(s) 37
PciSI GCTCTTC 1 cut(s) 159
PctI GAATGC 1 cut(s) 315
PfeI GAWTC 3 cut(s) 75, 447, 542
PkrI GCNGC 2 cut(s) 226, 259
PleI GAGTC 2 cut(s) 120, 1047
PpsI GAGTC 2 cut(s) 120, 1047
PsiI TTATAA 2 cut(s) 659, 1032
Psp6I CCWGG 4 cut(s) 683, 691, 1081, 1093
PspGI CCWGG 4 cut(s) 683, 691, 1081, 1093
PspN4I GGNNCC 3 cut(s) 690, 738, 1087
PspPI GGNCC 3 cut(s) 533, 695, 1085
PstNI CAGNNNCTG 1 cut(s) 257
PsuI RGATCY 1 cut(s) 736
RsaI GTAC 3 cut(s) 359, 715, 925
RsaNI GTAC 3 cut(s) 358, 714, 924
RseI CAYNNNNRTG 1 cut(s) 283
SapI GCTCTTC 1 cut(s) 159
SaqAI TTAA 2 cut(s) 32, 834
SatI GCNGC 2 cut(s) 225, 258
Sau3AI GATC 3 cut(s) 736, 817, 969
Sau96I GGNCC 3 cut(s) 533, 695, 1085
SchI GAGTC 2 cut(s) 120, 1047
ScrFI CCNGG 4 cut(s) 685, 693, 1083, 1095
SduI GDGCHC 1 cut(s) 789
SexAI ACCWGGT 2 cut(s) 691, 1081
SfaNI GCATC 3 cut(s) 194, 265, 792
SfcI CTRYAG 1 cut(s) 216
SfuI TTCGAA 1 cut(s) 512
SinI GGWCC 2 cut(s) 695, 1085
SmiMI CAYNNNNRTG 1 cut(s) 283
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
Sse9I AATT 6 cut(s) 191, 231, 261, 571, 720, 1178
SseBI AGGCCT 1 cut(s) 37
SsiI CCGC 1 cut(s) 871
SspI AATATT 3 cut(s) 171, 509, 1162
SspMI CTAG 1 cut(s) 414
StuI AGGCCT 1 cut(s) 37
StyD4I CCNGG 4 cut(s) 683, 691, 1081, 1093
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 4 cut(s) 556, 649, 827, 904
TaiI ACGT 1 cut(s) 490
TaqI TCGA 2 cut(s) 121, 512
TaqII GACCGA 1 cut(s) 4
TasI AATT 6 cut(s) 191, 231, 261, 571, 720, 1178
TatI WGTACW 1 cut(s) 713
TfiI GAWTC 3 cut(s) 75, 447, 542
Tru1I TTAA 2 cut(s) 32, 834
Tru9I TTAA 2 cut(s) 32, 834
TscAI CASTG 4 cut(s) 460, 654, 948, 979
TseFI GTSAC 2 cut(s) 162, 488
TseI GCWGC 2 cut(s) 224, 257
Tsp45I GTSAC 2 cut(s) 162, 488
TspDTI ATGAA 4 cut(s) 305, 427, 485, 860
TspGWI ACGGA 2 cut(s) 54, 741
TspRI CASTG 4 cut(s) 460, 654, 948, 979
Vha464I CTTAAG 1 cut(s) 31
VpaK11BI GGWCC 2 cut(s) 695, 1085
XbaI TCTAGA 1 cut(s) 413
XmiI GTMKAC 1 cut(s) 899
XspI CTAG 1 cut(s) 414
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.