Rw6G040010

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0004018
Physical Location & Seq
Reverse (-)
63838270 .. 63842004
3735 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G040010.1

Sequence Viewer

Length: 840 bp
ATGGCGTCTTTATCGGTCACTCTCAATCTCTTAAGGCCTTGTTCCATTGCTCCTAAGCCATCTCCGTCTTTGAGATTCTGTGATGTCCGCAACTCTGCTTCTTCCTATTGTTCTACTTCTCGAAGACTCGCTCTCTTTCACCTTGGCTCTGCTCTTCCTCAGTCACAAATATTAGGTGCAAAAGCATCTCAATTGCTGAGAGGAGAGGGGAAACCTGTAGAGGCTGCCCCAATTGCTGATTTGGCACAAGCAGGTGCTGCAATTGCTCAGGAAGATGCATTGGAATGGGTAAAAAATGATAAACGAAGAATGCTTCATGTCGTCTATCGTGTTGGGGACTTGGACAAGACTATAAAATTCTACACTGAGTGTTTGGGGATGAAGCTGTTGAGAAAACGTGACATATCCGAGGAGCAATATTCGAATGCTTTTCTTGGATATGGGCCAGAAGATTCCAACTTTACTGTTGAACTTACATATAATTATGGAGTGGACAAGTATGACATTGGGACTGGTTTTGGCCACTTTGGTATTGCAGTTGAAGATGTTGCCAAAACAGTGGAACTTATAAAGGCAAAGGGAGGGAAAGTTACCAGGGAACCTGGACCTGCCAATGGTGGCGGTACAATAATTGCTCTTATTGAGGATCCTGATGGTTATCCGTTTAAGCTTTTAGAGAAATGGCCAACACCAGAGCCCTTTTCACAAGTGATGCTTCGTGTAGGTGATCTTGACCGTGCTATTAACTTTTATACGAAGGCTTTTGGAATGGAACTTCTCCGCACAAGAGATAATCCCAAATACAAGGTAACCTCACTCTTACTACGTTCTGTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

279

Amino Acids

30.72

Weight (kDa)

8.75

Isoelectric Point (pI)

41.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 104 - 222 1.2e-25 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 106 - 211 1.4e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 235 - 272 3.5e-07 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 569
AarI CACCTGC 1 cut(s) 242
Acc36I ACCTGC 2 cut(s) 242, 616
AciI CCGC 3 cut(s) 88, 621, 781
AclWI GGATC 2 cut(s) 641, 654
AcoI YGGCCR 2 cut(s) 520, 683
AcsI RAATTY 1 cut(s) 356
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 369
AfaI GTAC 1 cut(s) 625
AfiI CCNNNNNNNGG 1 cut(s) 614
AflII CTTAAG 1 cut(s) 31
AgsI TTSAA 2 cut(s) 470, 542
AjnI CCWGG 2 cut(s) 593, 601
AluBI AGCT 2 cut(s) 385, 670
AluI AGCT 2 cut(s) 385, 670
AlwI GGATC 2 cut(s) 641, 654
AlwNI CAGNNNCTG 1 cut(s) 257
AoxI GGCC 4 cut(s) 35, 443, 520, 683
ApeKI GCWGC 2 cut(s) 224, 257
ApoI RAATTY 1 cut(s) 356
AspS9I GGNCC 2 cut(s) 443, 605
AsuHPI GGTGA 2 cut(s) 131, 737
AsuII TTCGAA 1 cut(s) 422
AvaII GGWCC 1 cut(s) 605
BalI TGGCCA 2 cut(s) 522, 685
BamHI GGATCC 1 cut(s) 646
BanII GRGCYC 1 cut(s) 699
BbsI GAAGAC 1 cut(s) 130
BbvI GCAGC 2 cut(s) 211, 244
BccI CCATC 2 cut(s) 67, 647
BciT130I CCWGG 2 cut(s) 595, 603
BfmI CTRYAG 1 cut(s) 216
BfrI CTTAAG 1 cut(s) 31
BfuAI ACCTGC 2 cut(s) 242, 616
BisI GCNGC 2 cut(s) 225, 258
BlsI GCNGC 2 cut(s) 226, 259
Bme1390I CCNGG 2 cut(s) 595, 603
Bme18I GGWCC 1 cut(s) 605
BmgT120I GGNCC 2 cut(s) 443, 605
BmiI GGNNCC 2 cut(s) 600, 648
BmrFI CCNGG 2 cut(s) 595, 603
BmsI GCATC 3 cut(s) 194, 265, 702
BpiI GAAGAC 1 cut(s) 130
Bpu10I CCTNAGC 2 cut(s) 54, 267
Bpu14I TTCGAA 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 657
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 142, 408, 594
BsaXI ACNNNNNCTCC 2 cut(s) 573, 603
Bsc4I CCNNNNNNNGG 1 cut(s) 614
Bse1I ACTGG 1 cut(s) 517
Bse3DI GCAATG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 657
BseBI CCWGG 2 cut(s) 595, 603
BseDI CCNNGG 3 cut(s) 142, 408, 594
BseGI GGATG 1 cut(s) 384
BseJI GATNNNNATC 1 cut(s) 657
BseLI CCNNNNNNNGG 1 cut(s) 614
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 4 cut(s) 173, 188, 281, 357
BseNI ACTGG 1 cut(s) 517
BseRI GAGGAG 2 cut(s) 216, 425
BseXI GCAGC 2 cut(s) 211, 244
BshFI GGCC 4 cut(s) 37, 445, 522, 685
BslFI GGGAC 2 cut(s) 350, 523
BslI CCNNNNNNNGG 1 cut(s) 614
BsmFI GGGAC 2 cut(s) 350, 523
BsmI GAATGC 2 cut(s) 315, 430
BsnI GGCC 4 cut(s) 37, 445, 522, 685
Bsp119I TTCGAA 1 cut(s) 422
Bsp1286I GDGCHC 1 cut(s) 699
Bsp143I GATC 2 cut(s) 646, 727
BspACI CCGC 3 cut(s) 88, 621, 781
BspANI GGCC 4 cut(s) 37, 445, 522, 685
BspCNI CTCAG 4 cut(s) 172, 189, 280, 358
BspLI GGNNCC 2 cut(s) 600, 648
BspMI ACCTGC 2 cut(s) 242, 616
BspPI GGATC 2 cut(s) 641, 654
BspQI GCTCTTC 1 cut(s) 159
BspT104I TTCGAA 1 cut(s) 422
BspTI CTTAAG 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 1 cut(s) 517
BssECI CCNNGG 3 cut(s) 142, 408, 594
BssMI GATC 2 cut(s) 646, 727
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 142
Bst2UI CCWGG 2 cut(s) 595, 603
Bst4CI ACNGT 3 cut(s) 466, 559, 737
Bst6I CTCTTC 1 cut(s) 159
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 31
BstAPI GCANNNNNTGC 1 cut(s) 257
BstBI TTCGAA 1 cut(s) 422
BstDEI CTNAG 5 cut(s) 54, 159, 197, 267, 366
BstEII GGTNACC 1 cut(s) 808
BstF5I GGATG 1 cut(s) 384
BstKTI GATC 2 cut(s) 649, 730
BstMBI GATC 2 cut(s) 646, 727
BstMWI GCNNNNNNNGC 4 cut(s) 233, 242, 257, 263
BstNI CCWGG 2 cut(s) 595, 603
BstPI GGTNACC 1 cut(s) 808
BstSCI CCNGG 2 cut(s) 593, 601
BstSFI CTRYAG 1 cut(s) 216
BstV1I GCAGC 2 cut(s) 211, 244
BstV2I GAAGAC 1 cut(s) 130
BstX2I RGATCY 1 cut(s) 646
BstXI CCANNNNNNTGG 1 cut(s) 559
BstYI RGATCY 1 cut(s) 646
BsuRI GGCC 4 cut(s) 37, 445, 522, 685
BtsCI GGATG 1 cut(s) 384
BtsIMutI CAGTG 2 cut(s) 363, 564
BveI ACCTGC 2 cut(s) 242, 616
CaiI CAGNNNCTG 1 cut(s) 257
Cfr13I GGNCC 2 cut(s) 443, 605
Csp6I GTAC 1 cut(s) 624
CviAII CATG 1 cut(s) 317
CviQI GTAC 1 cut(s) 624
DdeI CTNAG 5 cut(s) 54, 159, 197, 267, 366
DpnI GATC 2 cut(s) 648, 729
DpnII GATC 2 cut(s) 646, 727
DraIII CACNNNGTG 1 cut(s) 369
EaeI YGGCCR 2 cut(s) 520, 683
Eam1104I CTCTTC 1 cut(s) 159
EarI CTCTTC 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 142
Eco147I AGGCCT 1 cut(s) 37
Eco24I GRGCYC 1 cut(s) 699
Eco47I GGWCC 1 cut(s) 605
Eco91I GGTNACC 1 cut(s) 808
EcoO65I GGTNACC 1 cut(s) 808
EcoRII CCWGG 2 cut(s) 593, 601
EcoT14I CCWWGG 1 cut(s) 142
EcoT22I ATGCAT 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 699
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 1 cut(s) 320
FalI AAGNNNNNCTT 2 cut(s) 699, 731
FaqI GGGAC 2 cut(s) 350, 523
FatI CATG 1 cut(s) 316
Fnu4HI GCNGC 2 cut(s) 225, 258
FokI GGATG 1 cut(s) 391
FriOI GRGCYC 1 cut(s) 699
Fsp4HI GCNGC 2 cut(s) 225, 258
GluI GCNGC 2 cut(s) 225, 258
HaeIII GGCC 4 cut(s) 37, 445, 522, 685
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 320
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 3 cut(s) 75, 126, 452
HphI GGTGA 2 cut(s) 131, 737
Hpy166II GTNNAC 1 cut(s) 493
Hpy188I TCNGA 1 cut(s) 409
Hpy188III TCNNGA 4 cut(s) 120, 269, 650, 731
Hpy8I GTNNAC 1 cut(s) 493
HpyAV CCTTC 1 cut(s) 751
HpyCH4III ACNGT 3 cut(s) 466, 559, 737
HpyCH4IV ACGT 2 cut(s) 397, 826
HpyCH4V TGCA 4 cut(s) 179, 260, 278, 536
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 242, 257, 263
HpyF3I CTNAG 5 cut(s) 54, 159, 197, 267, 366
HpySE526I ACGT 2 cut(s) 397, 826
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 320
Kzo9I GATC 2 cut(s) 646, 727
LguI GCTCTTC 1 cut(s) 159
LmnI GCTCC 2 cut(s) 55, 412
Lsp1109I GCAGC 2 cut(s) 211, 244
LweI GCATC 3 cut(s) 194, 265, 702
MaeII ACGT 2 cut(s) 397, 826
MaeIII GTNAC 5 cut(s) 16, 162, 398, 589, 808
MalI GATC 2 cut(s) 648, 729
MboI GATC 2 cut(s) 646, 727
MboII GAAGA 7 cut(s) 93, 135, 146, 284, 318, 461, 554
MfeI CAATTG 3 cut(s) 191, 231, 261
MflI RGATCY 1 cut(s) 646
MhlI GDGCHC 1 cut(s) 699
MlsI TGGCCA 2 cut(s) 522, 685
MluCI AATT 6 cut(s) 191, 231, 261, 356, 481, 630
MluNI TGGCCA 2 cut(s) 522, 685
MlyI GAGTC 1 cut(s) 120
MmeI TCCRAC 1 cut(s) 480
MnlI CCTC 8 cut(s) 168, 194, 199, 214, 403, 575, 637, 823
Mox20I TGGCCA 2 cut(s) 522, 685
Mph1103I ATGCAT 1 cut(s) 280
MscI TGGCCA 2 cut(s) 522, 685
MseI TTAA 4 cut(s) 32, 666, 744, 838
MslI CAYNNNNRTG 1 cut(s) 283
Msp20I TGGCCA 2 cut(s) 522, 685
MspCI CTTAAG 1 cut(s) 31
MspR9I CCNGG 2 cut(s) 595, 603
MunI CAATTG 3 cut(s) 191, 231, 261
Mva1269I GAATGC 2 cut(s) 315, 430
MvaI CCWGG 2 cut(s) 595, 603
MwoI GCNNNNNNNGC 4 cut(s) 233, 242, 257, 263
NdeII GATC 2 cut(s) 646, 727
NlaIII CATG 1 cut(s) 320
NlaIV GGNNCC 2 cut(s) 600, 648
NmuCI GTSAC 3 cut(s) 16, 162, 398
NsiI ATGCAT 1 cut(s) 280
NspV TTCGAA 1 cut(s) 422
PaqCI CACCTGC 1 cut(s) 242
PceI AGGCCT 1 cut(s) 37
PciSI GCTCTTC 1 cut(s) 159
PctI GAATGC 2 cut(s) 315, 430
PfeI GAWTC 2 cut(s) 75, 452
PkrI GCNGC 2 cut(s) 226, 259
PleI GAGTC 1 cut(s) 120
PpsI GAGTC 1 cut(s) 120
PsiI TTATAA 1 cut(s) 569
Psp6I CCWGG 2 cut(s) 593, 601
PspEI GGTNACC 1 cut(s) 808
PspGI CCWGG 2 cut(s) 593, 601
PspN4I GGNNCC 2 cut(s) 600, 648
PspPI GGNCC 2 cut(s) 443, 605
PstNI CAGNNNCTG 1 cut(s) 257
PsuI RGATCY 1 cut(s) 646
RsaI GTAC 1 cut(s) 625
RsaNI GTAC 1 cut(s) 624
RseI CAYNNNNRTG 1 cut(s) 283
SapI GCTCTTC 1 cut(s) 159
SaqAI TTAA 4 cut(s) 32, 666, 744, 838
SatI GCNGC 2 cut(s) 225, 258
Sau3AI GATC 2 cut(s) 646, 727
Sau96I GGNCC 2 cut(s) 443, 605
SchI GAGTC 1 cut(s) 120
ScrFI CCNGG 2 cut(s) 595, 603
SduI GDGCHC 1 cut(s) 699
SfaNI GCATC 3 cut(s) 194, 265, 702
SfcI CTRYAG 1 cut(s) 216
SfuI TTCGAA 1 cut(s) 422
SinI GGWCC 1 cut(s) 605
SmiMI CAYNNNNRTG 1 cut(s) 283
SmlI CTYRAG 1 cut(s) 31
SmoI CTYRAG 1 cut(s) 31
Sse9I AATT 6 cut(s) 191, 231, 261, 356, 481, 630
SseBI AGGCCT 1 cut(s) 37
SsiI CCGC 3 cut(s) 88, 621, 781
SspI AATATT 2 cut(s) 171, 419
StuI AGGCCT 1 cut(s) 37
StyD4I CCNGG 2 cut(s) 593, 601
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 3 cut(s) 466, 559, 737
TaiI ACGT 2 cut(s) 400, 829
TaqI TCGA 2 cut(s) 121, 422
TaqII GACCGA 1 cut(s) 4
TasI AATT 6 cut(s) 191, 231, 261, 356, 481, 630
TfiI GAWTC 2 cut(s) 75, 452
Tru1I TTAA 4 cut(s) 32, 666, 744, 838
Tru9I TTAA 4 cut(s) 32, 666, 744, 838
TscAI CASTG 2 cut(s) 370, 564
TseFI GTSAC 3 cut(s) 16, 162, 398
TseI GCWGC 2 cut(s) 224, 257
Tsp45I GTSAC 3 cut(s) 16, 162, 398
TspDTI ATGAA 2 cut(s) 305, 395
TspGWI ACGGA 2 cut(s) 54, 651
TspRI CASTG 2 cut(s) 370, 564
Vha464I CTTAAG 1 cut(s) 31
VpaK11BI GGWCC 1 cut(s) 605
XapI RAATTY 1 cut(s) 356
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.