MD02G1058400.v1.1

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0004018
Physical Location & Seq
Reverse (-)
4687638 .. 4690584
2947 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1058400.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGTGGGGATAATTCCCATGGCGTCTTCAATCAGACCTTCGCTCTCGACGTTGAGGTTCGCCGGAGCTTCTCGCCTGGGCCTTTCCCTCGCTTCCTTCACTCCTTCAAGGCGACTCGCTTGCGTCCAACTCGCTAGTGCTGTGCCTCAGTCGCCGTCTTTCGGTCTGAAAGCTTCTAAGCTGTTGAGAGGAGATGGTAAAACCACGGGGGTAGCTGCTGCTGGAAATGCCGCACAAGCTAGCACGACAGCTTCCCCCGAAAATGTCCTTGAGTGGGTCAAACAGGACAAGCGGAGGATGCTCCATGTTGTTTACCGCGTTGGAGATTTGGACAGGACCATAAAATTCTACACAGAGTGCTTGGGAATGAAATTGTTGCGAAAACGTGACATACCCGAGGAAAGATATACCAATGCTTTTCTTGGGTACGGGCCAGAGGATTCCCACTTTGTTATTGAACTCACTTACAATTATGGAGTTGACAAGTATGATATTGGAACTGCATTTGGTCATTTTGGTATTGCTGTTGAGGATGTTGCCAAGACTGTGGAGCTTGTAAAAGCTAAGGGTGGCAAGGTAACTCGCGAACCTGGTCCAGTCAAAGGGGGCAAAACAGTAATCGCATTTGTTGAAGATCCTGATGGTTATAAGTTTGAACTTTTGGAAAGAGGGCCTACCCCAGAGCCCTTGTGTCAAGTTATGCTTCGTGTGGGTGATCTTGACCGATCTGTAACTTTTTATGAGAAGGCTTTTGGCATGGAGCTCCTTCGCAAAAGAGATAATCCGGAATACAAGTATACTATAGCAATTATGGGTTATGGCCCCGAAGACAAAAGTGCCATTCTGGAGTTGACATACAATTATGGGGTCACCGAATATGATAAAGGAAATGCTTATGCTCAGATAGCAATAGGCACAGATGATGTCTATAAAACTGCCGAAGCAATTAAACTCTGTGGGGGCAAGATTACCCGGGAACCTGGGCCATTACCTGGTCTCAACACAAAGATCACTGCTTGCTTGGATCCTGACGGATGGAAGTCGGTTTTCGTGGATAATGTTGATTTCCTCAAGGAATTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

362

Amino Acids

39.59

Weight (kDa)

7.55

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 100 - 220 6.4e-27 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 102 - 207 2.4e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 232 - 347 1.6e-18 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 648
AccB7I CCANNNNNTGG 1 cut(s) 992
AccI GTMKAC 1 cut(s) 797
AccII CGCG 2 cut(s) 318, 585
AccIII TCCGGA 1 cut(s) 784
AciI CCGC 3 cut(s) 231, 292, 316
AclWI GGATC 3 cut(s) 629, 1019, 1032
AcsI RAATTY 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 23
AdeI CACNNNGTG 1 cut(s) 357
AfaI GTAC 1 cut(s) 428
AfiI CCNNNNNNNGG 4 cut(s) 161, 274, 602, 992
AgsI TTSAA 5 cut(s) 30, 108, 458, 632, 656
AjnI CCWGG 4 cut(s) 75, 589, 979, 991
AluBI AGCT 9 cut(s) 68, 173, 181, 215, 239, 251, 553, 563, 763
AluI AGCT 9 cut(s) 68, 173, 181, 215, 239, 251, 553, 563, 763
Alw21I GWGCWC 1 cut(s) 765
Alw26I GTCTC 1 cut(s) 1001
AlwI GGATC 3 cut(s) 629, 1019, 1032
Ama87I CYCGRG 2 cut(s) 395, 972
Aor13HI TCCGGA 1 cut(s) 784
AoxI GGCC 5 cut(s) 79, 431, 671, 820, 983
ApeKI GCWGC 2 cut(s) 215, 218
ApoI RAATTY 1 cut(s) 344
AspS9I GGNCC 7 cut(s) 79, 336, 431, 593, 671, 821, 983
AsuC2I CCSGG 2 cut(s) 973, 974
AsuHPI GGTGA 2 cut(s) 725, 862
AsuNHI GCTAGC 1 cut(s) 239
AvaI CYCGRG 2 cut(s) 395, 972
AvaII GGWCC 2 cut(s) 336, 593
BamHI GGATCC 1 cut(s) 1024
BanII GRGCYC 2 cut(s) 687, 765
BbsI GAAGAC 2 cut(s) 18, 834
Bbv12I GWGCWC 1 cut(s) 765
BbvI GCAGC 2 cut(s) 202, 205
BccI CCATC 3 cut(s) 188, 635, 1029
BceAI ACGGC 1 cut(s) 139
BcgI CGANNNNNNTGC 2 cut(s) 102, 136
BciT130I CCWGG 4 cut(s) 77, 591, 981, 993
BcnI CCSGG 2 cut(s) 973, 974
BcoDI GTCTC 1 cut(s) 1001
BfaI CTAG 2 cut(s) 135, 240
BfmI CTRYAG 1 cut(s) 801
BisI GCNGC 3 cut(s) 216, 219, 231
BlsI GCNGC 3 cut(s) 217, 220, 232
Bme1390I CCNGG 6 cut(s) 77, 591, 973, 974, 981, 993
Bme18I GGWCC 2 cut(s) 336, 593
BmeT110I CYCGRG 2 cut(s) 395, 972
BmgT120I GGNCC 7 cut(s) 79, 336, 431, 593, 671, 821, 983
BmiI GGNNCC 3 cut(s) 823, 978, 1026
BmrFI CCNGG 6 cut(s) 77, 591, 973, 974, 981, 993
BmsI GCATC 1 cut(s) 288
BmtI GCTAGC 1 cut(s) 243
BpiI GAAGAC 2 cut(s) 18, 834
BpmI CTGGAG 1 cut(s) 866
Bpu10I CCTNAGC 1 cut(s) 564
BpuEI CTTGAG 2 cut(s) 290, 1055
BpuMI CCSGG 2 cut(s) 973, 974
BsaHI GRCGYC 1 cut(s) 23
BsaI GGTCTC 1 cut(s) 1001
BsaJI CCNNGG 6 cut(s) 18, 76, 204, 396, 972, 980
BsaWI WCCGGW 1 cut(s) 784
Bsc4I CCNNNNNNNGG 4 cut(s) 161, 274, 602, 992
Bse1I ACTGG 1 cut(s) 596
BseAI TCCGGA 1 cut(s) 784
BseBI CCWGG 4 cut(s) 77, 591, 981, 993
BseDI CCNNGG 6 cut(s) 18, 76, 204, 396, 972, 980
BseGI GGATG 3 cut(s) 303, 538, 1040
BseLI CCNNNNNNNGG 4 cut(s) 161, 274, 602, 992
BseMII CTCAG 2 cut(s) 161, 914
BseNI ACTGG 1 cut(s) 596
BseRI GAGGAG 1 cut(s) 204
BseXI GCAGC 2 cut(s) 202, 205
Bsh1236I CGCG 2 cut(s) 318, 585
BshFI GGCC 5 cut(s) 81, 433, 673, 822, 985
BsiHKAI GWGCWC 1 cut(s) 765
BsiHKCI CYCGRG 2 cut(s) 395, 972
BsiSI CCGG 3 cut(s) 63, 785, 973
BslI CCNNNNNNNGG 4 cut(s) 161, 274, 602, 992
BsmAI GTCTC 1 cut(s) 1001
BsnI GGCC 5 cut(s) 81, 433, 673, 822, 985
Bso31I GGTCTC 1 cut(s) 1001
BsoBI CYCGRG 2 cut(s) 395, 972
Bsp1286I GDGCHC 2 cut(s) 687, 765
Bsp13I TCCGGA 1 cut(s) 784
Bsp143I GATC 5 cut(s) 634, 715, 725, 1008, 1024
Bsp19I CCATGG 1 cut(s) 18
Bsp68I TCGCGA 1 cut(s) 585
BspACI CCGC 3 cut(s) 231, 292, 316
BspANI GGCC 5 cut(s) 81, 433, 673, 822, 985
BspCNI CTCAG 2 cut(s) 160, 913
BspEI TCCGGA 1 cut(s) 784
BspFNI CGCG 2 cut(s) 318, 585
BspLI GGNNCC 3 cut(s) 823, 978, 1026
BspOI GCTAGC 1 cut(s) 243
BspPI GGATC 3 cut(s) 629, 1019, 1032
BspTNI GGTCTC 1 cut(s) 1001
BsrI ACTGG 1 cut(s) 596
BssECI CCNNGG 6 cut(s) 18, 76, 204, 396, 972, 980
BssMI GATC 5 cut(s) 634, 715, 725, 1008, 1024
BssNAI GTATAC 1 cut(s) 798
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 1 cut(s) 18
Bst1107I GTATAC 1 cut(s) 798
Bst2UI CCWGG 4 cut(s) 77, 591, 981, 993
Bst4CI ACNGT 2 cut(s) 547, 616
BstACI GRCGYC 1 cut(s) 23
BstC8I GCNNGC 3 cut(s) 121, 241, 1018
BstDEI CTNAG 4 cut(s) 147, 177, 564, 900
BstDSI CCRYGG 2 cut(s) 18, 204
BstEII GGTNACC 1 cut(s) 868
BstF5I GGATG 3 cut(s) 303, 538, 1040
BstFNI CGCG 2 cut(s) 318, 585
BstKTI GATC 5 cut(s) 637, 718, 728, 1011, 1027
BstMAI GTCTC 1 cut(s) 1001
BstMBI GATC 5 cut(s) 634, 715, 725, 1008, 1024
BstMWI GCNNNNNNNGC 5 cut(s) 151, 227, 236, 298, 905
BstNI CCWGG 4 cut(s) 77, 591, 981, 993
BstPI GGTNACC 1 cut(s) 868
BstSCI CCNGG 6 cut(s) 75, 589, 971, 972, 979, 991
BstSFI CTRYAG 1 cut(s) 801
BstUI CGCG 2 cut(s) 318, 585
BstV1I GCAGC 2 cut(s) 202, 205
BstV2I GAAGAC 2 cut(s) 18, 834
BstX2I RGATCY 2 cut(s) 634, 1024
BstXI CCANNNNNNTGG 1 cut(s) 547
BstYI RGATCY 2 cut(s) 634, 1024
BstZ17I GTATAC 1 cut(s) 798
BsuRI GGCC 5 cut(s) 81, 433, 673, 822, 985
BtgI CCRYGG 2 cut(s) 18, 204
BtsCI GGATG 3 cut(s) 303, 538, 1040
BtsI GCAGTG 1 cut(s) 1011
BtsIMutI CAGTG 1 cut(s) 1011
BtuMI TCGCGA 1 cut(s) 585
Cac8I GCNNGC 3 cut(s) 121, 241, 1018
Cfr13I GGNCC 7 cut(s) 79, 336, 431, 593, 671, 821, 983
Cfr9I CCCGGG 1 cut(s) 972
CseI GACGC 2 cut(s) 12, 112
CsiI ACCWGGT 2 cut(s) 589, 991
Csp6I GTAC 1 cut(s) 427
CviAII CATG 3 cut(s) 19, 305, 757
CviQI GTAC 1 cut(s) 427
DdeI CTNAG 4 cut(s) 147, 177, 564, 900
DpnI GATC 5 cut(s) 636, 717, 727, 1010, 1026
DpnII GATC 5 cut(s) 634, 715, 725, 1008, 1024
DraIII CACNNNGTG 1 cut(s) 357
Ecl136II GAGCTC 1 cut(s) 763
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 2 cut(s) 687, 765
Eco31I GGTCTC 1 cut(s) 1001
Eco47I GGWCC 2 cut(s) 336, 593
Eco53kI GAGCTC 1 cut(s) 763
Eco88I CYCGRG 2 cut(s) 395, 972
Eco91I GGTNACC 1 cut(s) 868
EcoICRI GAGCTC 1 cut(s) 763
EcoO109I RGGNCCY 1 cut(s) 671
EcoO65I GGTNACC 1 cut(s) 868
EcoRII CCWGG 4 cut(s) 75, 589, 979, 991
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 2 cut(s) 687, 765
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 3 cut(s) 22, 308, 760
FalI AAGNNNNNCTT 2 cut(s) 687, 719
FatI CATG 3 cut(s) 18, 304, 756
FblI GTMKAC 1 cut(s) 797
Fnu4HI GCNGC 3 cut(s) 216, 219, 231
FokI GGATG 3 cut(s) 310, 545, 1047
FriOI GRGCYC 2 cut(s) 687, 765
Fsp4HI GCNGC 3 cut(s) 216, 219, 231
FspBI CTAG 2 cut(s) 135, 240
GluI GCNGC 3 cut(s) 216, 219, 231
GsuI CTGGAG 1 cut(s) 866
HaeIII GGCC 5 cut(s) 81, 433, 673, 822, 985
HapII CCGG 3 cut(s) 63, 785, 973
HgaI GACGC 2 cut(s) 12, 112
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 3 cut(s) 22, 308, 760
HincII GTYRAC 2 cut(s) 481, 852
HindII GTYRAC 2 cut(s) 481, 852
HindIII AAGCTT 1 cut(s) 171
HinfI GANTC 2 cut(s) 114, 440
HpaII CCGG 3 cut(s) 63, 785, 973
HphI GGTGA 2 cut(s) 725, 862
Hpy166II GTNNAC 4 cut(s) 313, 481, 798, 852
Hpy188I TCNGA 3 cut(s) 35, 168, 903
Hpy188III TCNNGA 7 cut(s) 46, 584, 638, 719, 785, 845, 1028
Hpy8I GTNNAC 4 cut(s) 313, 481, 798, 852
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 5 cut(s) 48, 106, 114, 739, 776
HpyCH4III ACNGT 2 cut(s) 547, 616
HpyCH4IV ACGT 2 cut(s) 50, 385
HpyCH4V TGCA 1 cut(s) 503
HpyF10VI GCNNNNNNNGC 5 cut(s) 151, 227, 236, 298, 905
HpyF3I CTNAG 4 cut(s) 147, 177, 564, 900
HpySE526I ACGT 2 cut(s) 50, 385
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 3 cut(s) 22, 308, 760
Kpn2I TCCGGA 1 cut(s) 784
Kzo9I GATC 5 cut(s) 634, 715, 725, 1008, 1024
LmnI GCTCC 5 cut(s) 65, 306, 550, 760, 768
Lsp1109I GCAGC 2 cut(s) 202, 205
LweI GCATC 1 cut(s) 288
MabI ACCWGGT 2 cut(s) 589, 991
MaeI CTAG 2 cut(s) 135, 240
MaeII ACGT 2 cut(s) 50, 385
MaeIII GTNAC 4 cut(s) 386, 577, 730, 868
MalI GATC 5 cut(s) 636, 717, 727, 1010, 1026
MboI GATC 5 cut(s) 634, 715, 725, 1008, 1024
MboII GAAGA 3 cut(s) 18, 644, 839
MflI RGATCY 2 cut(s) 634, 1024
MhlI GDGCHC 2 cut(s) 687, 765
MluCI AATT 8 cut(s) 12, 344, 371, 469, 807, 859, 945, 1076
MlyI GAGTC 1 cut(s) 108
MmeI TCCRAC 2 cut(s) 151, 301
MroI TCCGGA 1 cut(s) 784
MseI TTAA 1 cut(s) 948
MspI CCGG 3 cut(s) 63, 785, 973
MspR9I CCNGG 6 cut(s) 77, 591, 973, 974, 981, 993
MvaI CCWGG 4 cut(s) 77, 591, 981, 993
MvnI CGCG 2 cut(s) 318, 585
MwoI GCNNNNNNNGC 5 cut(s) 151, 227, 236, 298, 905
NciI CCSGG 2 cut(s) 973, 974
NcoI CCATGG 1 cut(s) 18
NdeII GATC 5 cut(s) 634, 715, 725, 1008, 1024
NheI GCTAGC 1 cut(s) 239
NlaIII CATG 3 cut(s) 22, 308, 760
NlaIV GGNNCC 3 cut(s) 823, 978, 1026
NmuCI GTSAC 2 cut(s) 386, 868
NruI TCGCGA 1 cut(s) 585
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PfeI GAWTC 1 cut(s) 440
PflMI CCANNNNNTGG 1 cut(s) 992
PkrI GCNGC 3 cut(s) 217, 220, 232
PleI GAGTC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 108
PsiI TTATAA 1 cut(s) 648
Psp124BI GAGCTC 1 cut(s) 765
Psp6I CCWGG 4 cut(s) 75, 589, 979, 991
PspEI GGTNACC 1 cut(s) 868
PspGI CCWGG 4 cut(s) 75, 589, 979, 991
PspN4I GGNNCC 3 cut(s) 823, 978, 1026
PspPI GGNCC 7 cut(s) 79, 336, 431, 593, 671, 821, 983
PsuI RGATCY 2 cut(s) 634, 1024
RruI TCGCGA 1 cut(s) 585
RsaI GTAC 1 cut(s) 428
RsaNI GTAC 1 cut(s) 427
SacI GAGCTC 1 cut(s) 765
SaqAI TTAA 1 cut(s) 948
SatI GCNGC 3 cut(s) 216, 219, 231
Sau3AI GATC 5 cut(s) 634, 715, 725, 1008, 1024
Sau96I GGNCC 7 cut(s) 79, 336, 431, 593, 671, 821, 983
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 6 cut(s) 77, 591, 973, 974, 981, 993
SduI GDGCHC 2 cut(s) 687, 765
SexAI ACCWGGT 2 cut(s) 589, 991
SfaNI GCATC 1 cut(s) 288
SfcI CTRYAG 1 cut(s) 801
SinI GGWCC 2 cut(s) 336, 593
SmaI CCCGGG 1 cut(s) 974
SmlI CTYRAG 2 cut(s) 269, 1070
SmoI CTYRAG 2 cut(s) 269, 1070
Sse9I AATT 8 cut(s) 12, 344, 371, 469, 807, 859, 945, 1076
SsiI CCGC 3 cut(s) 231, 292, 316
SspMI CTAG 2 cut(s) 135, 240
SstI GAGCTC 1 cut(s) 765
StyD4I CCNGG 6 cut(s) 75, 589, 971, 972, 979, 991
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 2 cut(s) 547, 616
TaiI ACGT 2 cut(s) 53, 388
TaqI TCGA 1 cut(s) 47
TaqII GACCGA 2 cut(s) 152, 738
TasI AATT 8 cut(s) 12, 344, 371, 469, 807, 859, 945, 1076
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 1 cut(s) 440
Tru1I TTAA 1 cut(s) 948
Tru9I TTAA 1 cut(s) 948
TscAI CASTG 1 cut(s) 1018
TseFI GTSAC 2 cut(s) 386, 868
TseI GCWGC 2 cut(s) 215, 218
Tsp45I GTSAC 2 cut(s) 386, 868
TspDTI ATGAA 1 cut(s) 383
TspGWI ACGGA 1 cut(s) 1047
TspMI CCCGGG 1 cut(s) 972
TspRI CASTG 1 cut(s) 1018
Van91I CCANNNNNTGG 1 cut(s) 992
VpaK11BI GGWCC 2 cut(s) 336, 593
XapI RAATTY 1 cut(s) 344
XmaI CCCGGG 1 cut(s) 972
XmiI GTMKAC 1 cut(s) 797
XspI CTAG 2 cut(s) 135, 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.