MD15G1192700.v1.1

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004018
Physical Location & Seq
Reverse (-)
15198590 .. 15201960
3371 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1192700.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGTGCGGATAATTCCCATGGCGTCTTCAATCAGACCCTCGCTATCCACGCTGAGGTTCGCCGGAGCTTCTCGCCTGGGCTTTCCCTTCCCTTCCTTCACTCCTTCCCGGCGACTCGCTTGCTTCCAACTCGCTAGTGCTGTGCCACAGTCGCCGTCTTTCGGTCTGAAAGCTTCTAAACTGTTGAGAGGAGATGGTAAAACCGCGGGGGTGGCTGCTGCTGGAAATGCCGCGCAAGCTAGCACAACAGCTTCCCCTGAAAATGTCCTAGAGTGGGTCAAACAGGACAAGCGGAGGATGCTCCATGTTGTTTACCGCGTTGGAGATTTGGACAGGACCATAAAATTCTACACAGAATGCTTAGGAATGAAATTGTTGCGAAAGCGTGACATACCCGAGGAAAGATACACCAATGCTTTCCTTGGGTACGGGCCAGAGGATTCCCACTTTGTTATTGAACTCACTTACAATTATGGAGTTGACAAGTATGATATTGGAACTGCATTTGGTCATTTTGGTATTGCCGTTGAGGATGTTGCCAAGACTGTGGAGCTCATAAAAGCTAAGGGTGGCAAGGTAACTCGTGAACCTGGTCCGGTCAAAGGGGGAAAAACAGTAATTGCATTTATTGAAGATCCAGATGGTTATAAGTTCGAACTATTGGAAAGAGGGCCTACCCCTGAGCCCTTGTGTCAAGTTATGCTTCGTGTGGGCAATCTTGATCGATCCATAACTTTTTATGAAAAGGCTTTTGGCATGGAGCTCCTTCGCAAAAGAGATAATCCGGAATACAAGTATACTATAGCAATGATGGGTTATGGCCCCGAAGACAAAAGTGCTGTTCTGGAGTTGACATACAATTACGGGGTCACCGAATATGACAAGGGAAATGCTTATGCTCAGATAGCAATAGGCACAGATGATGTCTATAAAACTGCTGAAGCAGTTAAACTCTCTGGGGGCAAGATTACTCGGGAGCCTGGGCCATTACCTGGTCTAAACACAAAGATCACTGCTTGCTTGGACCCTGACGGATGGAAATCGGTTTTTGTGGATAACGTTGATTTCCTCAAGGAATTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00620 map00620
Pfam Domains
Protein Families
Glyoxalase

Protein Analysis

362

Amino Acids

39.83

Weight (kDa)

8.44

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 100 - 220 4.4e-27 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 102 - 207 1.8e-10 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase PF00903 232 - 347 2.6e-19 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 648
AccB7I CCANNNNNTGG 1 cut(s) 992
AccI GTMKAC 1 cut(s) 797
AccII CGCG 3 cut(s) 206, 233, 318
AccIII TCCGGA 1 cut(s) 784
AciI CCGC 6 cut(s) 7, 204, 206, 231, 292, 316
AclI AACGTT 1 cut(s) 1059
AclWI GGATC 2 cut(s) 629, 720
AcsI RAATTY 1 cut(s) 344
AcuI CTGAAG 1 cut(s) 960
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 1 cut(s) 428
AfiI CCNNNNNNNGG 5 cut(s) 54, 161, 274, 602, 992
AgsI TTSAA 3 cut(s) 30, 458, 632
AjnI CCWGG 4 cut(s) 75, 589, 979, 991
AjuI GAANNNNNNNTTGG 2 cut(s) 735, 767
AluBI AGCT 7 cut(s) 68, 173, 239, 251, 553, 563, 763
AluI AGCT 7 cut(s) 68, 173, 239, 251, 553, 563, 763
Alw21I GWGCWC 2 cut(s) 555, 765
AlwI GGATC 2 cut(s) 629, 720
Ama87I CYCGRG 2 cut(s) 395, 972
Aor13HI TCCGGA 1 cut(s) 784
AoxI GGCC 4 cut(s) 431, 671, 820, 983
ApeKI GCWGC 2 cut(s) 215, 218
ApoI RAATTY 1 cut(s) 344
AspLEI GCGC 1 cut(s) 235
AspS9I GGNCC 7 cut(s) 336, 431, 593, 671, 821, 983, 1024
AsuC2I CCSGG 1 cut(s) 109
AsuHPI GGTGA 1 cut(s) 862
AsuII TTCGAA 1 cut(s) 654
AsuNHI GCTAGC 1 cut(s) 239
AvaI CYCGRG 2 cut(s) 395, 972
AvaII GGWCC 3 cut(s) 336, 593, 1024
BanII GRGCYC 3 cut(s) 555, 687, 765
BauI CACGAG 1 cut(s) 582
BbsI GAAGAC 2 cut(s) 18, 834
Bbv12I GWGCWC 2 cut(s) 555, 765
BbvCI CCTCAGC 1 cut(s) 53
BbvI GCAGC 2 cut(s) 202, 205
BccI CCATC 4 cut(s) 188, 635, 805, 1029
BceAI ACGGC 2 cut(s) 139, 509
BcgI CGANNNNNNTGC 2 cut(s) 102, 136
BciT130I CCWGG 4 cut(s) 77, 591, 981, 993
BcnI CCSGG 1 cut(s) 109
BfaI CTAG 3 cut(s) 135, 240, 269
BfmI CTRYAG 1 cut(s) 801
BisI GCNGC 3 cut(s) 216, 219, 231
BlsI GCNGC 3 cut(s) 217, 220, 232
Bme1390I CCNGG 5 cut(s) 77, 109, 591, 981, 993
Bme18I GGWCC 3 cut(s) 336, 593, 1024
BmeT110I CYCGRG 2 cut(s) 395, 972
BmgT120I GGNCC 7 cut(s) 336, 431, 593, 671, 821, 983, 1024
BmiI GGNNCC 3 cut(s) 823, 978, 1026
BmrFI CCNGG 5 cut(s) 77, 109, 591, 981, 993
BmsI GCATC 1 cut(s) 288
BmtI GCTAGC 1 cut(s) 243
BpiI GAAGAC 2 cut(s) 18, 834
BpmI CTGGAG 1 cut(s) 866
Bpu10I CCTNAGC 4 cut(s) 53, 361, 564, 681
Bpu14I TTCGAA 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 1055
BpuMI CCSGG 1 cut(s) 109
Bsa29I ATCGAT 1 cut(s) 724
BsaBI GATNNNNATC 1 cut(s) 1039
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 6 cut(s) 18, 76, 204, 396, 421, 980
BsaWI WCCGGW 2 cut(s) 595, 784
Bsc4I CCNNNNNNNGG 5 cut(s) 54, 161, 274, 602, 992
Bse3DI GCAATG 1 cut(s) 813
Bse8I GATNNNNATC 1 cut(s) 1039
BseAI TCCGGA 1 cut(s) 784
BseBI CCWGG 4 cut(s) 77, 591, 981, 993
BseCI ATCGAT 1 cut(s) 724
BseDI CCNNGG 6 cut(s) 18, 76, 204, 396, 421, 980
BseGI GGATG 3 cut(s) 303, 538, 1040
BseJI GATNNNNATC 1 cut(s) 1039
BseLI CCNNNNNNNGG 5 cut(s) 54, 161, 274, 602, 992
BseMI GCAATG 1 cut(s) 813
BseMII CTCAG 3 cut(s) 44, 672, 914
BseRI GAGGAG 1 cut(s) 204
BseXI GCAGC 2 cut(s) 202, 205
Bsh1236I CGCG 3 cut(s) 206, 233, 318
BshFI GGCC 4 cut(s) 433, 673, 822, 985
BshVI ATCGAT 1 cut(s) 724
BsiHKAI GWGCWC 2 cut(s) 555, 765
BsiHKCI CYCGRG 2 cut(s) 395, 972
BsiSI CCGG 4 cut(s) 63, 109, 596, 785
BslI CCNNNNNNNGG 5 cut(s) 54, 161, 274, 602, 992
BsmI GAATGC 1 cut(s) 362
BsnI GGCC 4 cut(s) 433, 673, 822, 985
BsoBI CYCGRG 2 cut(s) 395, 972
Bsp119I TTCGAA 1 cut(s) 654
Bsp1286I GDGCHC 3 cut(s) 555, 687, 765
Bsp13I TCCGGA 1 cut(s) 784
Bsp143I GATC 4 cut(s) 634, 721, 725, 1008
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 6 cut(s) 7, 204, 206, 231, 292, 316
BspANI GGCC 4 cut(s) 433, 673, 822, 985
BspCNI CTCAG 3 cut(s) 45, 673, 913
BspDI ATCGAT 1 cut(s) 724
BspEI TCCGGA 1 cut(s) 784
BspFNI CGCG 3 cut(s) 206, 233, 318
BspLI GGNNCC 3 cut(s) 823, 978, 1026
BspOI GCTAGC 1 cut(s) 243
BspPI GGATC 2 cut(s) 629, 720
BspT104I TTCGAA 1 cut(s) 654
BsrDI GCAATG 1 cut(s) 813
BssECI CCNNGG 6 cut(s) 18, 76, 204, 396, 421, 980
BssMI GATC 4 cut(s) 634, 721, 725, 1008
BssNAI GTATAC 1 cut(s) 798
BssNI GRCGYC 1 cut(s) 23
BssSI CACGAG 1 cut(s) 582
BssT1I CCWWGG 2 cut(s) 18, 421
Bst1107I GTATAC 1 cut(s) 798
Bst2BI CACGAG 1 cut(s) 582
Bst2UI CCWGG 4 cut(s) 77, 591, 981, 993
Bst4CI ACNGT 4 cut(s) 150, 183, 547, 616
BstACI GRCGYC 1 cut(s) 23
BstBI TTCGAA 1 cut(s) 654
BstC8I GCNNGC 4 cut(s) 121, 237, 241, 1018
BstDEI CTNAG 5 cut(s) 53, 361, 564, 681, 900
BstDSI CCRYGG 2 cut(s) 18, 204
BstEII GGTNACC 1 cut(s) 868
BstF5I GGATG 3 cut(s) 303, 538, 1040
BstFNI CGCG 3 cut(s) 206, 233, 318
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 4 cut(s) 637, 724, 728, 1011
BstMBI GATC 4 cut(s) 634, 721, 725, 1008
BstMWI GCNNNNNNNGC 7 cut(s) 49, 151, 212, 227, 236, 298, 905
BstNI CCWGG 4 cut(s) 77, 591, 981, 993
BstPI GGTNACC 1 cut(s) 868
BstSCI CCNGG 5 cut(s) 75, 107, 589, 979, 991
BstSFI CTRYAG 1 cut(s) 801
BstUI CGCG 3 cut(s) 206, 233, 318
BstV1I GCAGC 2 cut(s) 202, 205
BstV2I GAAGAC 2 cut(s) 18, 834
BstX2I RGATCY 1 cut(s) 634
BstXI CCANNNNNNTGG 1 cut(s) 547
BstYI RGATCY 1 cut(s) 634
BstZ17I GTATAC 1 cut(s) 798
Bsu15I ATCGAT 1 cut(s) 724
BsuRI GGCC 4 cut(s) 433, 673, 822, 985
BsuTUI ATCGAT 1 cut(s) 724
BtgI CCRYGG 2 cut(s) 18, 204
BtsCI GGATG 3 cut(s) 303, 538, 1040
BtsI GCAGTG 1 cut(s) 1011
BtsIMutI CAGTG 1 cut(s) 1011
Cac8I GCNNGC 4 cut(s) 121, 237, 241, 1018
CfoI GCGC 1 cut(s) 235
Cfr13I GGNCC 7 cut(s) 336, 431, 593, 671, 821, 983, 1024
Cfr42I CCGCGG 1 cut(s) 207
ClaI ATCGAT 1 cut(s) 724
CseI GACGC 1 cut(s) 12
CsiI ACCWGGT 2 cut(s) 589, 991
Csp6I GTAC 1 cut(s) 427
CviAII CATG 3 cut(s) 19, 305, 757
CviQI GTAC 1 cut(s) 427
DdeI CTNAG 5 cut(s) 53, 361, 564, 681, 900
DpnI GATC 4 cut(s) 636, 723, 727, 1010
DpnII GATC 4 cut(s) 634, 721, 725, 1008
Ecl136II GAGCTC 2 cut(s) 553, 763
Eco130I CCWWGG 2 cut(s) 18, 421
Eco24I GRGCYC 3 cut(s) 555, 687, 765
Eco47I GGWCC 3 cut(s) 336, 593, 1024
Eco53kI GAGCTC 2 cut(s) 553, 763
Eco57I CTGAAG 1 cut(s) 960
Eco88I CYCGRG 2 cut(s) 395, 972
Eco91I GGTNACC 1 cut(s) 868
EcoICRI GAGCTC 2 cut(s) 553, 763
EcoO109I RGGNCCY 1 cut(s) 671
EcoO65I GGTNACC 1 cut(s) 868
EcoRII CCWGG 4 cut(s) 75, 589, 979, 991
EcoT14I CCWWGG 2 cut(s) 18, 421
EcoT38I GRGCYC 3 cut(s) 555, 687, 765
ErhI CCWWGG 2 cut(s) 18, 421
FaeI CATG 3 cut(s) 22, 308, 760
FalI AAGNNNNNCTT 2 cut(s) 687, 719
FatI CATG 3 cut(s) 18, 304, 756
FauI CCCGC 1 cut(s) 199
FblI GTMKAC 1 cut(s) 797
Fnu4HI GCNGC 3 cut(s) 216, 219, 231
FokI GGATG 3 cut(s) 310, 545, 1047
FriOI GRGCYC 3 cut(s) 555, 687, 765
Fsp4HI GCNGC 3 cut(s) 216, 219, 231
FspBI CTAG 3 cut(s) 135, 240, 269
GlaI GCGC 1 cut(s) 234
GluI GCNGC 3 cut(s) 216, 219, 231
GsuI CTGGAG 1 cut(s) 866
HaeIII GGCC 4 cut(s) 433, 673, 822, 985
HapII CCGG 4 cut(s) 63, 109, 596, 785
HgaI GACGC 1 cut(s) 12
HhaI GCGC 1 cut(s) 235
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 3 cut(s) 22, 308, 760
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HincII GTYRAC 2 cut(s) 481, 852
HindII GTYRAC 2 cut(s) 481, 852
HindIII AAGCTT 1 cut(s) 171
HinfI GANTC 2 cut(s) 114, 440
HpaII CCGG 4 cut(s) 63, 109, 596, 785
HphI GGTGA 1 cut(s) 862
Hpy166II GTNNAC 5 cut(s) 313, 481, 587, 798, 852
Hpy188I TCNGA 3 cut(s) 35, 168, 903
Hpy188III TCNNGA 6 cut(s) 584, 638, 719, 785, 845, 974
Hpy8I GTNNAC 5 cut(s) 313, 481, 587, 798, 852
HpyAV CCTTC 5 cut(s) 97, 102, 106, 114, 776
HpyCH4III ACNGT 4 cut(s) 150, 183, 547, 616
HpyCH4IV ACGT 1 cut(s) 1059
HpyCH4V TGCA 2 cut(s) 503, 623
HpyF10VI GCNNNNNNNGC 7 cut(s) 49, 151, 212, 227, 236, 298, 905
HpyF3I CTNAG 5 cut(s) 53, 361, 564, 681, 900
HpySE526I ACGT 1 cut(s) 1059
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 3 cut(s) 22, 308, 760
HspAI GCGC 1 cut(s) 233
Kpn2I TCCGGA 1 cut(s) 784
KspI CCGCGG 1 cut(s) 207
Kzo9I GATC 4 cut(s) 634, 721, 725, 1008
LmnI GCTCC 6 cut(s) 65, 306, 550, 760, 768, 976
Lsp1109I GCAGC 2 cut(s) 202, 205
LweI GCATC 1 cut(s) 288
MabI ACCWGGT 2 cut(s) 589, 991
MaeI CTAG 3 cut(s) 135, 240, 269
MaeII ACGT 1 cut(s) 1059
MaeIII GTNAC 3 cut(s) 386, 577, 868
MalI GATC 4 cut(s) 636, 723, 727, 1010
MboI GATC 4 cut(s) 634, 721, 725, 1008
MboII GAAGA 3 cut(s) 18, 644, 839
MflI RGATCY 1 cut(s) 634
MhlI GDGCHC 3 cut(s) 555, 687, 765
MluCI AATT 7 cut(s) 12, 344, 371, 469, 618, 859, 1076
MlyI GAGTC 1 cut(s) 108
MmeI TCCRAC 2 cut(s) 151, 301
MnlI CCTC 9 cut(s) 48, 49, 182, 288, 391, 430, 523, 662, 1079
MroI TCCGGA 1 cut(s) 784
MseI TTAA 1 cut(s) 948
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 4 cut(s) 63, 109, 596, 785
MspR9I CCNGG 5 cut(s) 77, 109, 591, 981, 993
Mva1269I GAATGC 1 cut(s) 362
MvaI CCWGG 4 cut(s) 77, 591, 981, 993
MvnI CGCG 3 cut(s) 206, 233, 318
MwoI GCNNNNNNNGC 7 cut(s) 49, 151, 212, 227, 236, 298, 905
NciI CCSGG 1 cut(s) 109
NcoI CCATGG 1 cut(s) 18
NdeII GATC 4 cut(s) 634, 721, 725, 1008
NheI GCTAGC 1 cut(s) 239
NlaIII CATG 3 cut(s) 22, 308, 760
NlaIV GGNNCC 3 cut(s) 823, 978, 1026
NmuCI GTSAC 2 cut(s) 386, 868
NspV TTCGAA 1 cut(s) 654
PcsI WCGNNNNNNNCGW 1 cut(s) 870
PctI GAATGC 1 cut(s) 362
PfeI GAWTC 1 cut(s) 440
PflMI CCANNNNNTGG 1 cut(s) 992
PkrI GCNGC 3 cut(s) 217, 220, 232
PleI GAGTC 1 cut(s) 108
PpsI GAGTC 1 cut(s) 108
PsiI TTATAA 1 cut(s) 648
Psp124BI GAGCTC 2 cut(s) 555, 765
Psp1406I AACGTT 1 cut(s) 1059
Psp6I CCWGG 4 cut(s) 75, 589, 979, 991
PspEI GGTNACC 1 cut(s) 868
PspGI CCWGG 4 cut(s) 75, 589, 979, 991
PspN4I GGNNCC 3 cut(s) 823, 978, 1026
PspPI GGNCC 7 cut(s) 336, 431, 593, 671, 821, 983, 1024
PsuI RGATCY 1 cut(s) 634
RsaI GTAC 1 cut(s) 428
RsaNI GTAC 1 cut(s) 427
SacI GAGCTC 2 cut(s) 555, 765
SacII CCGCGG 1 cut(s) 207
SaqAI TTAA 1 cut(s) 948
SatI GCNGC 3 cut(s) 216, 219, 231
Sau3AI GATC 4 cut(s) 634, 721, 725, 1008
Sau96I GGNCC 7 cut(s) 336, 431, 593, 671, 821, 983, 1024
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 5 cut(s) 77, 109, 591, 981, 993
SduI GDGCHC 3 cut(s) 555, 687, 765
SexAI ACCWGGT 2 cut(s) 589, 991
SfaNI GCATC 1 cut(s) 288
SfcI CTRYAG 1 cut(s) 801
Sfr303I CCGCGG 1 cut(s) 207
SfuI TTCGAA 1 cut(s) 654
SgrBI CCGCGG 1 cut(s) 207
SinI GGWCC 3 cut(s) 336, 593, 1024
SmlI CTYRAG 1 cut(s) 1070
SmoI CTYRAG 1 cut(s) 1070
Sse9I AATT 7 cut(s) 12, 344, 371, 469, 618, 859, 1076
SsiI CCGC 6 cut(s) 7, 204, 206, 231, 292, 316
SspMI CTAG 3 cut(s) 135, 240, 269
SstI GAGCTC 2 cut(s) 555, 765
StyD4I CCNGG 5 cut(s) 75, 107, 589, 979, 991
StyI CCWWGG 2 cut(s) 18, 421
TaaI ACNGT 4 cut(s) 150, 183, 547, 616
TaiI ACGT 1 cut(s) 1062
TaqI TCGA 2 cut(s) 654, 724
TaqII GACCGA 1 cut(s) 152
TasI AATT 7 cut(s) 12, 344, 371, 469, 618, 859, 1076
TauI GCSGC 1 cut(s) 233
TfiI GAWTC 1 cut(s) 440
Tru1I TTAA 1 cut(s) 948
Tru9I TTAA 1 cut(s) 948
TscAI CASTG 1 cut(s) 1018
TseFI GTSAC 2 cut(s) 386, 868
TseI GCWGC 2 cut(s) 215, 218
Tsp45I GTSAC 2 cut(s) 386, 868
TspDTI ATGAA 2 cut(s) 383, 756
TspGWI ACGGA 1 cut(s) 1047
TspRI CASTG 1 cut(s) 1018
Van91I CCANNNNNTGG 1 cut(s) 992
VpaK11BI GGWCC 3 cut(s) 336, 593, 1024
XapI RAATTY 1 cut(s) 344
XmiI GTMKAC 1 cut(s) 797
XspI CTAG 3 cut(s) 135, 240, 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.