FvH4_1g06480

May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004629
Physical Location & Seq
Reverse (-)
3416410 .. 3419417
3008 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06480.t1

Sequence Viewer

Length: 744 bp
ATGTCGACGCCTGCTGAATTCTATCAGTCACTCCCACCCATAAGCAAGGCTTACGGGACCATCTGTGTAGTGGCAACTGCGGCCTTTCACTTGGGGCTTTATGATCCTCGAACAATTGCATTATTATATGGACCAGTATTTTCACAGTTCCAGGTGTGGAGGTTGATTACAAATTTCTTTTTCCTCGGGAAGTTCTCTGTCAACTTTGGAATTCGCCTTTTAATGATAGCAAGATATGGAGTTCAACTTGAGAAGGGACCATTTGAACGGCGTACTGCTGATTTCTTGTGGATGATGATATTTGGAGCCTTGACACTATTGGTTTTATCCGCCATCCCAATGTTTTGGTCTCCATTCTTGGGGATATCTCTTGTGTTCATGCTTGTTTATGTCTGGAGTAGAGAATATCCAAATGCTGTTATCAACTTATATGGCCTCGTCCAACTTAAGGCATTTTATTTCCCCTGGGCGATGCTTGCTTTGGACGTCATCTTTGGTTCACCAATTTGGCCAGATCTGCTGGGGATCATTGCTGGACATCTGTACCACTTCTTGACCGTGTTGCATCCATTGGCAGGTGGAAAGAACATATTGAAGACTCCAAGATGGGTACATAAAATAGTTGCAAGATATAGGATAGGTGCTCCTCCACCAACAAGCCGATCCCAACCTACTGGCGCTGCTGCTGCTGCTCCTGGTACTGGTGCGGCTTTCAGAGGGAGGTCTTATCGACTTAATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DER1

Protein Analysis

248

Amino Acids

27.69

Weight (kDa)

10.04

Isoelectric Point (pI)

31.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DER1 PF04511 11 - 201 1e-45 Der1-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 566
AatII GACGTC 1 cut(s) 489
Acc36I ACCTGC 1 cut(s) 566
AccI GTMKAC 1 cut(s) 5
AciI CCGC 3 cut(s) 80, 330, 707
AclWI GGATC 3 cut(s) 98, 533, 657
AcoI YGGCCR 1 cut(s) 509
AcsI RAATTY 3 cut(s) 17, 172, 210
AcyI GRCGYC 2 cut(s) 8, 486
AfaI GTAC 4 cut(s) 274, 545, 612, 700
AfiI CCNNNNNNNGG 4 cut(s) 359, 448, 575, 701
AflII CTTAAG 1 cut(s) 446
AgsI TTSAA 3 cut(s) 245, 266, 595
AjnI CCWGG 3 cut(s) 150, 464, 694
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 1 cut(s) 354
AlwI GGATC 3 cut(s) 98, 533, 657
Ama87I CYCGRG 1 cut(s) 185
AoxI GGCC 3 cut(s) 81, 433, 509
ApeKI GCWGC 4 cut(s) 680, 683, 686, 689
ApoI RAATTY 3 cut(s) 17, 172, 210
ArsI GACNNNNNNTTYG 2 cut(s) 476, 508
AspLEI GCGC 1 cut(s) 680
AspS9I GGNCC 3 cut(s) 57, 131, 257
AsuHPI GGTGA 1 cut(s) 492
AvaI CYCGRG 1 cut(s) 185
AvaII GGWCC 3 cut(s) 57, 131, 257
BaeI ACNNNNGTAYC 2 cut(s) 527, 560
BalI TGGCCA 1 cut(s) 511
BbsI GAAGAC 1 cut(s) 602
Bbv12I GWGCWC 1 cut(s) 646
BbvI GCAGC 4 cut(s) 667, 670, 673, 676
BccI CCATC 3 cut(s) 68, 341, 600
BceAI ACGGC 1 cut(s) 284
BciT130I CCWGG 3 cut(s) 152, 466, 696
BcoDI GTCTC 1 cut(s) 354
BfoI RGCGCY 1 cut(s) 681
BfrI CTTAAG 1 cut(s) 446
BfuAI ACCTGC 1 cut(s) 566
BglII AGATCT 1 cut(s) 514
BisI GCNGC 6 cut(s) 81, 681, 684, 687, 690, 708
BlsI GCNGC 6 cut(s) 82, 682, 685, 688, 691, 709
Bme1390I CCNGG 3 cut(s) 152, 466, 696
Bme18I GGWCC 3 cut(s) 57, 131, 257
BmeT110I CYCGRG 1 cut(s) 185
BmgT120I GGNCC 3 cut(s) 57, 131, 257
BmiI GGNNCC 3 cut(s) 58, 258, 307
BmrFI CCNGG 3 cut(s) 152, 466, 696
BmsI GCATC 2 cut(s) 462, 574
BpiI GAAGAC 1 cut(s) 602
BpmI CTGGAG 1 cut(s) 415
BpuEI CTTGAG 1 cut(s) 269
BsaHI GRCGYC 2 cut(s) 8, 486
BsaI GGTCTC 1 cut(s) 354
BsaJI CCNNGG 3 cut(s) 184, 464, 465
Bsc4I CCNNNNNNNGG 4 cut(s) 359, 448, 575, 701
Bse1I ACTGG 3 cut(s) 134, 679, 706
Bse3DI GCAATG 1 cut(s) 528
BseBI CCWGG 3 cut(s) 152, 466, 696
BseDI CCNNGG 3 cut(s) 184, 464, 465
BseGI GGATG 3 cut(s) 297, 333, 565
BseLI CCNNNNNNNGG 4 cut(s) 359, 448, 575, 701
BseMI GCAATG 1 cut(s) 528
BseNI ACTGG 3 cut(s) 134, 679, 706
BseRI GAGGAG 1 cut(s) 636
BseXI GCAGC 4 cut(s) 667, 670, 673, 676
BseYI CCCAGC 1 cut(s) 520
BshFI GGCC 3 cut(s) 83, 435, 511
BsiHKAI GWGCWC 1 cut(s) 646
BsiHKCI CYCGRG 1 cut(s) 185
BslFI GGGAC 2 cut(s) 70, 270
BslI CCNNNNNNNGG 4 cut(s) 359, 448, 575, 701
BsmAI GTCTC 1 cut(s) 354
BsmFI GGGAC 2 cut(s) 70, 270
BsnI GGCC 3 cut(s) 83, 435, 511
Bso31I GGTCTC 1 cut(s) 354
BsoBI CYCGRG 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 646
Bsp143I GATC 4 cut(s) 103, 514, 525, 662
BspACI CCGC 3 cut(s) 80, 330, 707
BspANI GGCC 3 cut(s) 83, 435, 511
BspLI GGNNCC 3 cut(s) 58, 258, 307
BspMI ACCTGC 1 cut(s) 566
BspPI GGATC 3 cut(s) 98, 533, 657
BspTI CTTAAG 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 354
BsrDI GCAATG 1 cut(s) 528
BsrI ACTGG 3 cut(s) 134, 679, 706
BssECI CCNNGG 3 cut(s) 184, 464, 465
BssMI GATC 4 cut(s) 103, 514, 525, 662
BssNI GRCGYC 2 cut(s) 8, 486
Bst2UI CCWGG 3 cut(s) 152, 466, 696
Bst4CI ACNGT 2 cut(s) 147, 559
BstACI GRCGYC 2 cut(s) 8, 486
BstAFI CTTAAG 1 cut(s) 446
BstC8I GCNNGC 2 cut(s) 12, 477
BstF5I GGATG 3 cut(s) 297, 333, 565
BstH2I RGCGCY 1 cut(s) 681
BstHHI GCGC 1 cut(s) 680
BstKTI GATC 4 cut(s) 106, 517, 528, 665
BstMAI GTCTC 1 cut(s) 354
BstMBI GATC 4 cut(s) 103, 514, 525, 662
BstMWI GCNNNNNNNGC 5 cut(s) 80, 476, 517, 686, 689
BstNI CCWGG 3 cut(s) 152, 466, 696
BstSCI CCNGG 3 cut(s) 150, 464, 694
BstV1I GCAGC 4 cut(s) 667, 670, 673, 676
BstV2I GAAGAC 1 cut(s) 602
BstX2I RGATCY 1 cut(s) 514
BstXI CCANNNNNNTGG 2 cut(s) 345, 674
BstYI RGATCY 1 cut(s) 514
BsuRI GGCC 3 cut(s) 83, 435, 511
BtgZI GCGATG 1 cut(s) 485
BtsCI GGATG 3 cut(s) 297, 333, 565
BveI ACCTGC 1 cut(s) 566
Cac8I GCNNGC 2 cut(s) 12, 477
CfoI GCGC 1 cut(s) 680
Cfr13I GGNCC 3 cut(s) 57, 131, 257
CseI GACGC 1 cut(s) 16
Csp6I GTAC 4 cut(s) 273, 544, 611, 699
CviAII CATG 1 cut(s) 379
CviJI RGCY 8 cut(s) 50, 83, 97, 308, 435, 511, 660, 710
CviKI_1 RGCY 8 cut(s) 50, 83, 97, 308, 435, 511, 660, 710
CviQI GTAC 4 cut(s) 273, 544, 611, 699
DpnI GATC 4 cut(s) 105, 516, 527, 664
DpnII GATC 4 cut(s) 103, 514, 525, 662
EaeI YGGCCR 1 cut(s) 509
EciI GGCGGA 1 cut(s) 319
Eco31I GGTCTC 1 cut(s) 354
Eco32I GATATC 1 cut(s) 366
Eco47I GGWCC 3 cut(s) 57, 131, 257
Eco88I CYCGRG 1 cut(s) 185
EcoRI GAATTC 2 cut(s) 17, 210
EcoRII CCWGG 3 cut(s) 150, 464, 694
EcoRV GATATC 1 cut(s) 366
FaeI CATG 1 cut(s) 382
FalI AAGNNNNNCTT 2 cut(s) 34, 66
FaqI GGGAC 2 cut(s) 70, 270
FatI CATG 1 cut(s) 378
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 6 cut(s) 81, 681, 684, 687, 690, 708
FokI GGATG 3 cut(s) 304, 320, 552
Fsp4HI GCNGC 6 cut(s) 81, 681, 684, 687, 690, 708
GlaI GCGC 1 cut(s) 679
GluI GCNGC 6 cut(s) 81, 681, 684, 687, 690, 708
GsaI CCCAGC 1 cut(s) 524
GsuI CTGGAG 1 cut(s) 415
HaeII RGCGCY 1 cut(s) 681
HaeIII GGCC 3 cut(s) 83, 435, 511
HgaI GACGC 1 cut(s) 16
HhaI GCGC 1 cut(s) 680
Hin1I GRCGYC 2 cut(s) 8, 486
Hin1II CATG 1 cut(s) 382
Hin6I GCGC 1 cut(s) 678
HinP1I GCGC 1 cut(s) 678
HincII GTYRAC 2 cut(s) 6, 202
HindII GTYRAC 2 cut(s) 6, 202
HinfI GANTC 1 cut(s) 598
HphI GGTGA 1 cut(s) 492
Hpy166II GTNNAC 3 cut(s) 6, 202, 500
Hpy188I TCNGA 1 cut(s) 716
Hpy188III TCNNGA 3 cut(s) 187, 394, 553
Hpy8I GTNNAC 3 cut(s) 6, 202, 500
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 247
HpyCH4III ACNGT 2 cut(s) 147, 559
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 3 cut(s) 119, 565, 626
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 476, 517, 686, 689
HpySE526I ACGT 1 cut(s) 486
Hsp92I GRCGYC 2 cut(s) 8, 486
Hsp92II CATG 1 cut(s) 382
HspAI GCGC 1 cut(s) 678
Kzo9I GATC 4 cut(s) 103, 514, 525, 662
LmnI GCTCC 3 cut(s) 305, 649, 697
Lsp1109I GCAGC 4 cut(s) 667, 670, 673, 676
LweI GCATC 2 cut(s) 462, 574
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 1 cut(s) 27
MalI GATC 4 cut(s) 105, 516, 527, 664
MboI GATC 4 cut(s) 103, 514, 525, 662
MboII GAAGA 1 cut(s) 607
MfeI CAATTG 1 cut(s) 114
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 1 cut(s) 646
MlsI TGGCCA 1 cut(s) 511
MluCI AATT 5 cut(s) 17, 114, 172, 210, 504
MluNI TGGCCA 1 cut(s) 511
MlyI GAGTC 1 cut(s) 592
MmeI TCCRAC 1 cut(s) 466
MnlI CCTC 7 cut(s) 117, 153, 194, 446, 657, 710, 714
Mox20I TGGCCA 1 cut(s) 511
MscI TGGCCA 1 cut(s) 511
MseI TTAA 4 cut(s) 221, 447, 735, 742
MslI CAYNNNNRTG 1 cut(s) 338
Msp20I TGGCCA 1 cut(s) 511
MspCI CTTAAG 1 cut(s) 446
MspR9I CCNGG 3 cut(s) 152, 466, 696
MunI CAATTG 1 cut(s) 114
MvaI CCWGG 3 cut(s) 152, 466, 696
MwoI GCNNNNNNNGC 5 cut(s) 80, 476, 517, 686, 689
NdeII GATC 4 cut(s) 103, 514, 525, 662
NlaIII CATG 1 cut(s) 382
NlaIV GGNNCC 3 cut(s) 58, 258, 307
NmuCI GTSAC 1 cut(s) 27
PaqCI CACCTGC 1 cut(s) 566
PasI CCCWGGG 1 cut(s) 465
PkrI GCNGC 6 cut(s) 82, 682, 685, 688, 691, 709
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
Psp6I CCWGG 3 cut(s) 150, 464, 694
PspFI CCCAGC 1 cut(s) 520
PspGI CCWGG 3 cut(s) 150, 464, 694
PspN4I GGNNCC 3 cut(s) 58, 258, 307
PspPI GGNCC 3 cut(s) 57, 131, 257
PsuI RGATCY 1 cut(s) 514
RsaI GTAC 4 cut(s) 274, 545, 612, 700
RsaNI GTAC 4 cut(s) 273, 544, 611, 699
RseI CAYNNNNRTG 1 cut(s) 338
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 4 cut(s) 221, 447, 735, 742
SatI GCNGC 6 cut(s) 81, 681, 684, 687, 690, 708
Sau3AI GATC 4 cut(s) 103, 514, 525, 662
Sau96I GGNCC 3 cut(s) 57, 131, 257
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 3 cut(s) 152, 466, 696
SduI GDGCHC 1 cut(s) 646
SetI ASST 7 cut(s) 156, 164, 489, 580, 643, 673, 725
SfaNI GCATC 2 cut(s) 462, 574
SinI GGWCC 3 cut(s) 57, 131, 257
SmiMI CAYNNNNRTG 1 cut(s) 338
SmlI CTYRAG 2 cut(s) 248, 446
SmoI CTYRAG 2 cut(s) 248, 446
Sse9I AATT 5 cut(s) 17, 114, 172, 210, 504
SsiI CCGC 3 cut(s) 80, 330, 707
StyD4I CCNGG 3 cut(s) 150, 464, 694
TaaI ACNGT 2 cut(s) 147, 559
TaiI ACGT 1 cut(s) 489
TaqI TCGA 3 cut(s) 5, 109, 730
TasI AATT 5 cut(s) 17, 114, 172, 210, 504
TauI GCSGC 2 cut(s) 83, 710
Tru1I TTAA 4 cut(s) 221, 447, 735, 742
Tru9I TTAA 4 cut(s) 221, 447, 735, 742
TseFI GTSAC 1 cut(s) 27
TseI GCWGC 4 cut(s) 680, 683, 686, 689
Tsp45I GTSAC 1 cut(s) 27
TspDTI ATGAA 1 cut(s) 367
Vha464I CTTAAG 1 cut(s) 446
VpaK11BI GGWCC 3 cut(s) 57, 131, 257
XapI RAATTY 3 cut(s) 17, 172, 210
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XmiI GTMKAC 1 cut(s) 5
ZraI GACGTC 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.