Rroxscaffold_2G00149350

May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004629
Physical Location & Seq
Forward (+)
86950373 .. 86952893
2521 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149350.1

Sequence Viewer

Length: 738 bp
ATGTCTTCTCCTGCTGAATTCTATCAGTCACTCCCACCCATAAGCAAGGCCTACGGGACCATCTGTGTAGTGGCAACTGCGGCCTTTCAATTGGGACTTTATGATCCTCGAACTATTGCATTATTATATGGACCAGTATTTTCACAGTTCCAGGTGTGGAGGTTGATTACAAATTTCTTTTTCCTTGGGAAGTTCTCTGTCAACTTTGGAATTCGCCTTTTAATGATAGCAAGATATGGAGTTCAACTTGAAAAGGGACCATTTGAACGGCGTACTGCTGATTTCTTGTGGATGATGATATTTGGAGCCTTGACATTATTGGTTTTATCCGCCATCCCACTGTTTTGGTCTCCATTCTTGGGGGTATCTCTTGTGTTCATGCTTGTTTATGTCTGGAGTAGAGAATATCCGAATGCTAATATCAACTTATATGGCCTCGTGCAACTTAAGGCATTTTATTTCCCCTGGGTGATGCTTGCTTTGGACGTCATATTTGGTTCACCAATTTGGCCAGATCTGCTGGGAATCATTGCTGGACATCTGTACCACTTCTTGACCGTGTTGCATCCATTGGCAGGTGGAAAGAACATATTGAAGACTCCAAGATGGGTACATAAAATAGTTGCAAGATGGAGGATAGGTGCTCCTCCACCAACAAGCCGATCCCAACCTGCTGCCGCTCCCGCTCCCGGTACTGGTGTGGCTTTCAGAGGGAGGTCTTATCGACTTGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DER1

Protein Analysis

245

Amino Acids

27.59

Weight (kDa)

10.0

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DER1 PF04511 11 - 201 2.3e-46 Der1-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 566
AatII GACGTC 1 cut(s) 489
Acc36I ACCTGC 2 cut(s) 566, 679
AccBSI CCGCTC 2 cut(s) 680, 686
AciI CCGC 4 cut(s) 80, 330, 678, 684
AclWI GGATC 2 cut(s) 98, 657
AcoI YGGCCR 1 cut(s) 509
AcsI RAATTY 3 cut(s) 17, 172, 210
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 4 cut(s) 274, 545, 612, 694
AfiI CCNNNNNNNGG 4 cut(s) 359, 575, 689, 695
AflII CTTAAG 1 cut(s) 446
AgsI TTSAA 5 cut(s) 89, 245, 251, 266, 595
AjnI CCWGG 2 cut(s) 150, 464
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 1 cut(s) 354
AlwI GGATC 2 cut(s) 98, 657
AoxI GGCC 4 cut(s) 48, 81, 433, 509
ApeKI GCWGC 1 cut(s) 674
ApoI RAATTY 3 cut(s) 17, 172, 210
ArsI GACNNNNNNTTYG 2 cut(s) 476, 508
AspS9I GGNCC 3 cut(s) 57, 131, 257
AsuC2I CCSGG 1 cut(s) 690
AsuHPI GGTGA 2 cut(s) 481, 492
AvaII GGWCC 3 cut(s) 57, 131, 257
BaeI ACNNNNGTAYC 4 cut(s) 527, 560, 684, 717
BalI TGGCCA 1 cut(s) 511
BauI CACGAG 1 cut(s) 437
BbsI GAAGAC 1 cut(s) 602
Bbv12I GWGCWC 1 cut(s) 646
BbvI GCAGC 1 cut(s) 661
BccI CCATC 4 cut(s) 68, 341, 600, 624
BceAI ACGGC 1 cut(s) 284
BciT130I CCWGG 2 cut(s) 152, 466
BcnI CCSGG 1 cut(s) 690
BcoDI GTCTC 1 cut(s) 354
BfrI CTTAAG 1 cut(s) 446
BfuAI ACCTGC 2 cut(s) 566, 679
BglII AGATCT 1 cut(s) 514
BisI GCNGC 3 cut(s) 81, 675, 678
BlsI GCNGC 3 cut(s) 82, 676, 679
Bme1390I CCNGG 3 cut(s) 152, 466, 690
Bme18I GGWCC 3 cut(s) 57, 131, 257
BmgT120I GGNCC 3 cut(s) 57, 131, 257
BmiI GGNNCC 3 cut(s) 58, 258, 307
BmrFI CCNGG 3 cut(s) 152, 466, 690
BmsI GCATC 2 cut(s) 462, 574
BpiI GAAGAC 1 cut(s) 602
BpmI CTGGAG 1 cut(s) 415
BpuMI CCSGG 1 cut(s) 690
BsaHI GRCGYC 1 cut(s) 486
BsaI GGTCTC 1 cut(s) 354
BsaJI CCNNGG 3 cut(s) 184, 464, 465
BsaXI ACNNNNNCTCC 2 cut(s) 625, 655
Bsc4I CCNNNNNNNGG 4 cut(s) 359, 575, 689, 695
Bse1I ACTGG 2 cut(s) 134, 700
Bse3DI GCAATG 1 cut(s) 528
BseBI CCWGG 2 cut(s) 152, 466
BseDI CCNNGG 3 cut(s) 184, 464, 465
BseGI GGATG 3 cut(s) 297, 333, 565
BseLI CCNNNNNNNGG 4 cut(s) 359, 575, 689, 695
BseMI GCAATG 1 cut(s) 528
BseNI ACTGG 2 cut(s) 134, 700
BseRI GAGGAG 1 cut(s) 636
BseXI GCAGC 1 cut(s) 661
BseYI CCCAGC 1 cut(s) 520
BshFI GGCC 4 cut(s) 50, 83, 435, 511
BsiHKAI GWGCWC 1 cut(s) 646
BsiSI CCGG 1 cut(s) 690
BslFI GGGAC 3 cut(s) 70, 108, 270
BslI CCNNNNNNNGG 4 cut(s) 359, 575, 689, 695
BsmAI GTCTC 1 cut(s) 354
BsmFI GGGAC 3 cut(s) 70, 108, 270
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 4 cut(s) 50, 83, 435, 511
Bso31I GGTCTC 1 cut(s) 354
Bsp1286I GDGCHC 1 cut(s) 646
Bsp143I GATC 3 cut(s) 103, 514, 662
BspACI CCGC 4 cut(s) 80, 330, 678, 684
BspANI GGCC 4 cut(s) 50, 83, 435, 511
BspLI GGNNCC 3 cut(s) 58, 258, 307
BspMI ACCTGC 2 cut(s) 566, 679
BspPI GGATC 2 cut(s) 98, 657
BspTI CTTAAG 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 354
BsrBI CCGCTC 2 cut(s) 680, 686
BsrDI GCAATG 1 cut(s) 528
BsrI ACTGG 2 cut(s) 134, 700
BssECI CCNNGG 3 cut(s) 184, 464, 465
BssMI GATC 3 cut(s) 103, 514, 662
BssNI GRCGYC 1 cut(s) 486
BssSI CACGAG 1 cut(s) 437
BssT1I CCWWGG 1 cut(s) 184
Bst2BI CACGAG 1 cut(s) 437
Bst2UI CCWGG 2 cut(s) 152, 466
Bst4CI ACNGT 3 cut(s) 147, 342, 559
BstACI GRCGYC 1 cut(s) 486
BstAFI CTTAAG 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 477
BstF5I GGATG 3 cut(s) 297, 333, 565
BstKTI GATC 3 cut(s) 106, 517, 665
BstMAI GTCTC 1 cut(s) 354
BstMBI GATC 3 cut(s) 103, 514, 662
BstMWI GCNNNNNNNGC 3 cut(s) 80, 517, 683
BstNI CCWGG 2 cut(s) 152, 466
BstSCI CCNGG 3 cut(s) 150, 464, 688
BstV1I GCAGC 1 cut(s) 661
BstV2I GAAGAC 1 cut(s) 602
BstX2I RGATCY 1 cut(s) 514
BstXI CCANNNNNNTGG 1 cut(s) 345
BstYI RGATCY 1 cut(s) 514
BsuRI GGCC 4 cut(s) 50, 83, 435, 511
BtsCI GGATG 3 cut(s) 297, 333, 565
BtsIMutI CAGTG 1 cut(s) 338
BveI ACCTGC 2 cut(s) 566, 679
Cac8I GCNNGC 1 cut(s) 477
Cfr13I GGNCC 3 cut(s) 57, 131, 257
Csp6I GTAC 4 cut(s) 273, 544, 611, 693
CviAII CATG 1 cut(s) 379
CviJI RGCY 7 cut(s) 50, 83, 308, 435, 511, 660, 704
CviKI_1 RGCY 7 cut(s) 50, 83, 308, 435, 511, 660, 704
CviQI GTAC 4 cut(s) 273, 544, 611, 693
DpnI GATC 3 cut(s) 105, 516, 664
DpnII GATC 3 cut(s) 103, 514, 662
EaeI YGGCCR 1 cut(s) 509
EciI GGCGGA 1 cut(s) 319
Eco130I CCWWGG 1 cut(s) 184
Eco147I AGGCCT 1 cut(s) 50
Eco31I GGTCTC 1 cut(s) 354
Eco47I GGWCC 3 cut(s) 57, 131, 257
EcoRI GAATTC 2 cut(s) 17, 210
EcoRII CCWGG 2 cut(s) 150, 464
EcoT14I CCWWGG 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 184
FaeI CATG 1 cut(s) 382
FaqI GGGAC 3 cut(s) 70, 108, 270
FatI CATG 1 cut(s) 378
FauI CCCGC 1 cut(s) 691
Fnu4HI GCNGC 3 cut(s) 81, 675, 678
FokI GGATG 3 cut(s) 304, 320, 552
Fsp4HI GCNGC 3 cut(s) 81, 675, 678
GluI GCNGC 3 cut(s) 81, 675, 678
GsaI CCCAGC 1 cut(s) 524
GsuI CTGGAG 1 cut(s) 415
HaeIII GGCC 4 cut(s) 50, 83, 435, 511
HapII CCGG 1 cut(s) 690
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 1 cut(s) 382
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 2 cut(s) 525, 598
HpaII CCGG 1 cut(s) 690
HphI GGTGA 2 cut(s) 481, 492
Hpy166II GTNNAC 2 cut(s) 202, 500
Hpy188I TCNGA 2 cut(s) 411, 710
Hpy188III TCNNGA 2 cut(s) 394, 553
Hpy8I GTNNAC 2 cut(s) 202, 500
HpyCH4III ACNGT 3 cut(s) 147, 342, 559
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 4 cut(s) 119, 442, 565, 626
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 517, 683
HpySE526I ACGT 1 cut(s) 486
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 1 cut(s) 382
Kzo9I GATC 3 cut(s) 103, 514, 662
LmnI GCTCC 4 cut(s) 305, 649, 685, 691
Lsp1109I GCAGC 1 cut(s) 661
LweI GCATC 2 cut(s) 462, 574
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 1 cut(s) 27
MalI GATC 3 cut(s) 105, 516, 664
MbiI CCGCTC 2 cut(s) 680, 686
MboI GATC 3 cut(s) 103, 514, 662
MboII GAAGA 1 cut(s) 607
MfeI CAATTG 1 cut(s) 89
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 1 cut(s) 646
MlsI TGGCCA 1 cut(s) 511
MluCI AATT 5 cut(s) 17, 89, 172, 210, 504
MluNI TGGCCA 1 cut(s) 511
MlyI GAGTC 1 cut(s) 592
MnlI CCTC 7 cut(s) 117, 153, 446, 627, 657, 704, 708
Mox20I TGGCCA 1 cut(s) 511
MscI TGGCCA 1 cut(s) 511
MseI TTAA 3 cut(s) 221, 447, 736
Msp20I TGGCCA 1 cut(s) 511
MspCI CTTAAG 1 cut(s) 446
MspI CCGG 1 cut(s) 690
MspR9I CCNGG 3 cut(s) 152, 466, 690
MunI CAATTG 1 cut(s) 89
Mva1269I GAATGC 1 cut(s) 418
MvaI CCWGG 2 cut(s) 152, 466
MwoI GCNNNNNNNGC 3 cut(s) 80, 517, 683
NciI CCSGG 1 cut(s) 690
NdeII GATC 3 cut(s) 103, 514, 662
NlaIII CATG 1 cut(s) 382
NlaIV GGNNCC 3 cut(s) 58, 258, 307
NmuCI GTSAC 1 cut(s) 27
PaqCI CACCTGC 1 cut(s) 566
PasI CCCWGGG 1 cut(s) 465
PceI AGGCCT 1 cut(s) 50
PctI GAATGC 1 cut(s) 418
PfeI GAWTC 1 cut(s) 525
PkrI GCNGC 3 cut(s) 82, 676, 679
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
Psp6I CCWGG 2 cut(s) 150, 464
PspFI CCCAGC 1 cut(s) 520
PspGI CCWGG 2 cut(s) 150, 464
PspN4I GGNNCC 3 cut(s) 58, 258, 307
PspPI GGNCC 3 cut(s) 57, 131, 257
PsuI RGATCY 1 cut(s) 514
RsaI GTAC 4 cut(s) 274, 545, 612, 694
RsaNI GTAC 4 cut(s) 273, 544, 611, 693
SaqAI TTAA 3 cut(s) 221, 447, 736
SatI GCNGC 3 cut(s) 81, 675, 678
Sau3AI GATC 3 cut(s) 103, 514, 662
Sau96I GGNCC 3 cut(s) 57, 131, 257
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 3 cut(s) 152, 466, 690
SduI GDGCHC 1 cut(s) 646
SetI ASST 7 cut(s) 156, 164, 489, 580, 643, 673, 719
SfaNI GCATC 2 cut(s) 462, 574
SinI GGWCC 3 cut(s) 57, 131, 257
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 5 cut(s) 17, 89, 172, 210, 504
SseBI AGGCCT 1 cut(s) 50
SsiI CCGC 4 cut(s) 80, 330, 678, 684
StuI AGGCCT 1 cut(s) 50
StyD4I CCNGG 3 cut(s) 150, 464, 688
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 3 cut(s) 147, 342, 559
TaiI ACGT 1 cut(s) 489
TaqI TCGA 2 cut(s) 109, 724
TasI AATT 5 cut(s) 17, 89, 172, 210, 504
TauI GCSGC 2 cut(s) 83, 680
TfiI GAWTC 1 cut(s) 525
Tru1I TTAA 3 cut(s) 221, 447, 736
Tru9I TTAA 3 cut(s) 221, 447, 736
TscAI CASTG 1 cut(s) 345
TseFI GTSAC 1 cut(s) 27
TseI GCWGC 1 cut(s) 674
Tsp45I GTSAC 1 cut(s) 27
TspDTI ATGAA 1 cut(s) 367
TspRI CASTG 1 cut(s) 345
Vha464I CTTAAG 1 cut(s) 446
VpaK11BI GGWCC 3 cut(s) 57, 131, 257
XapI RAATTY 3 cut(s) 17, 172, 210
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
ZraI GACGTC 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.