MD07G1249900.v1.1

May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004629
Physical Location & Seq
Reverse (-)
31870048 .. 31873841
3794 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1249900.v1.1.491

Sequence Viewer

Length: 861 bp
ATGTCTACGCCTGGGGAATACTATCGATCTCTACCGCCGGTGAGCAAGACGTATGGAGTCGGATGTTTTATGTTTGCAGCTGCCTTGTACTTCCAACTTTATAATCCTTGGAACATAGCCCTTGATTATGGCCTTGTCATAAAACGTTTCCAGGTTTGGAGGCTTGTTACCAATTTCTTCTTTCTCGGACCCTTTTCCTTCCCGTTTGCTTGCGATCTTATAATGATAGCAAGATATGGTGTTGCATTGGAGAGAGGACCGTTTGACAAGAGAACGGCAGACTTTTTGTGGATGCTTATGTTTGGAGCTCTCTCACTTTTGGCTATGGCTGCAGTTCCATATTTATGGTCTCCGTTTATGGGAACTTCTGTGGTTTTCATGATCGTCTATGTATGGTCTCGTGAGTTCCCAAATTCACGTGTCAACATAGCTGGTGTTGTGTCCTTTAAGGGGTTCTATCTTCCCTACGTACTGCTGACTCTGGATTTGATGTTTGGAGTTCCTTTGAAGCCAGATATTATGGGGATGGTTGCAGGTCATCTCTATTACTTTTTAACTGTGCTTCATCCTCTTTCTGGTGGAAAATTCACTCTCAAGACTCCTCTCTGGGTACACAAATTAGTAGCCTTCTGGGGTCAGGGAATCCAAGTCAATTCCCCAGTGGAGCGCAATCCACAAGCCGGAGTTGCATTCCGAGGAAGAAGCTTTCGACTCAACGGGAGTACTAGAACAAGCACCCCAGTGCAGCCACAAACAAGCACCCCAGTGCAGCCACAAACAAGCAGCGGGACTACTACCACCACACAGCAGGCTGATGGAGTTGCTTTCCGTGGGACAGGCAGGCGCCTCAACGATCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DER1

Protein Analysis

287

Amino Acids

32.06

Weight (kDa)

9.88

Isoelectric Point (pI)

38.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DER1 PF04511 11 - 201 7e-42 Der1-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 102, 221
Acc36I ACCTGC 1 cut(s) 524
AccB1I GGYRCC 1 cut(s) 843
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 35, 786
AclI AACGTT 1 cut(s) 145
AclWI GGATC 1 cut(s) 848
AcsI RAATTY 2 cut(s) 412, 584
AcvI CACGTG 1 cut(s) 419
AcyI GRCGYC 1 cut(s) 844
AfaI GTAC 4 cut(s) 89, 471, 612, 724
AfiI CCNNNNNNNGG 4 cut(s) 359, 450, 575, 680
AflIII ACRYGT 1 cut(s) 418
AgsI TTSAA 1 cut(s) 508
AjnI CCWGG 2 cut(s) 10, 150
AleI CACNNNNGTG 2 cut(s) 740, 764
AluBI AGCT 4 cut(s) 80, 308, 431, 705
AluI AGCT 4 cut(s) 80, 308, 431, 705
Alw21I GWGCWC 1 cut(s) 310
Alw26I GTCTC 2 cut(s) 354, 402
AlwI GGATC 1 cut(s) 848
AoxI GGCC 1 cut(s) 130
ApeKI GCWGC 6 cut(s) 77, 80, 329, 745, 769, 783
ApoI RAATTY 2 cut(s) 412, 584
ArsI GACNNNNNNTTYG 2 cut(s) 469, 501
AspLEI GCGC 2 cut(s) 669, 846
AspS9I GGNCC 2 cut(s) 188, 257
AsuHPI GGTGA 1 cut(s) 52
AvaII GGWCC 2 cut(s) 188, 257
BanI GGYRCC 1 cut(s) 843
BanII GRGCYC 1 cut(s) 310
BauI CACGAG 1 cut(s) 399
BbrPI CACGTG 1 cut(s) 419
Bbv12I GWGCWC 1 cut(s) 310
BbvI GCAGC 6 cut(s) 67, 89, 316, 757, 781, 795
BccI CCATC 2 cut(s) 520, 809
BceAI ACGGC 1 cut(s) 291
BciT130I CCWGG 2 cut(s) 12, 152
BcoDI GTCTC 2 cut(s) 354, 402
BfaI CTAG 1 cut(s) 726
BfmI CTRYAG 1 cut(s) 330
BfoI RGCGCY 1 cut(s) 847
BfuAI ACCTGC 1 cut(s) 524
BisI GCNGC 6 cut(s) 78, 81, 330, 746, 770, 784
BlsI GCNGC 6 cut(s) 79, 82, 331, 747, 771, 785
BmcAI AGTACT 1 cut(s) 724
Bme1390I CCNGG 2 cut(s) 12, 152
Bme18I GGWCC 2 cut(s) 188, 257
BmgT120I GGNCC 2 cut(s) 188, 257
BmiI GGNNCC 2 cut(s) 190, 845
BmrFI CCNGG 2 cut(s) 12, 152
BmrI ACTGGG 3 cut(s) 653, 734, 758
BmsI GCATC 1 cut(s) 282
BmuI ACTGGG 3 cut(s) 653, 734, 758
BpuEI CTTGAG 1 cut(s) 578
Bsa29I ATCGAT 1 cut(s) 25
BsaAI YACGTR 2 cut(s) 419, 469
BsaHI GRCGYC 1 cut(s) 844
BsaI GGTCTC 2 cut(s) 354, 402
BsaJI CCNNGG 4 cut(s) 11, 107, 694, 829
Bsc4I CCNNNNNNNGG 4 cut(s) 359, 450, 575, 680
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 3 cut(s) 659, 740, 764
BseBI CCWGG 2 cut(s) 12, 152
BseCI ATCGAT 1 cut(s) 25
BseDI CCNNGG 4 cut(s) 11, 107, 694, 829
BseGI GGATG 4 cut(s) 68, 297, 531, 565
BseLI CCNNNNNNNGG 4 cut(s) 359, 450, 575, 680
BseNI ACTGG 3 cut(s) 659, 740, 764
BseRI GAGGAG 1 cut(s) 591
BseXI GCAGC 6 cut(s) 67, 89, 316, 757, 781, 795
BsgI GTGCAG 2 cut(s) 764, 788
BshFI GGCC 1 cut(s) 132
BshNI GGYRCC 1 cut(s) 843
BshVI ATCGAT 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 310
BsiSI CCGG 2 cut(s) 38, 681
BslFI GGGAC 2 cut(s) 802, 847
BslI CCNNNNNNNGG 4 cut(s) 359, 450, 575, 680
BsmAI GTCTC 2 cut(s) 354, 402
BsmFI GGGAC 2 cut(s) 802, 847
BsmI GAATGC 1 cut(s) 689
BsnI GGCC 1 cut(s) 132
Bso31I GGTCTC 2 cut(s) 354, 402
Bsp1286I GDGCHC 1 cut(s) 310
Bsp143I GATC 4 cut(s) 26, 214, 381, 853
BspACI CCGC 2 cut(s) 35, 786
BspANI GGCC 1 cut(s) 132
BspDI ATCGAT 1 cut(s) 25
BspHI TCATGA 1 cut(s) 378
BspLI GGNNCC 2 cut(s) 190, 845
BspMAI CTGCAG 1 cut(s) 334
BspMI ACCTGC 1 cut(s) 524
BspPI GGATC 1 cut(s) 848
BspT107I GGYRCC 1 cut(s) 843
BspTNI GGTCTC 2 cut(s) 354, 402
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 3 cut(s) 659, 740, 764
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 4 cut(s) 11, 107, 694, 829
BssMI GATC 4 cut(s) 26, 214, 381, 853
BssNI GRCGYC 1 cut(s) 844
BssSI CACGAG 1 cut(s) 399
BssT1I CCWWGG 1 cut(s) 107
Bst2BI CACGAG 1 cut(s) 399
Bst2UI CCWGG 2 cut(s) 12, 152
Bst4CI ACNGT 2 cut(s) 261, 559
BstACI GRCGYC 1 cut(s) 844
BstBAI YACGTR 2 cut(s) 419, 469
BstC8I GCNNGC 3 cut(s) 211, 810, 842
BstDSI CCRYGG 1 cut(s) 829
BstF5I GGATG 4 cut(s) 68, 297, 531, 565
BstH2I RGCGCY 1 cut(s) 847
BstHHI GCGC 2 cut(s) 669, 846
BstKTI GATC 4 cut(s) 29, 217, 384, 856
BstMAI GTCTC 2 cut(s) 354, 402
BstMBI GATC 4 cut(s) 26, 214, 381, 853
BstMWI GCNNNNNNNGC 2 cut(s) 329, 686
BstNI CCWGG 2 cut(s) 12, 152
BstSCI CCNGG 2 cut(s) 10, 150
BstSFI CTRYAG 1 cut(s) 330
BstSNI TACGTA 1 cut(s) 469
BstV1I GCAGC 6 cut(s) 67, 89, 316, 757, 781, 795
BstXI CCANNNNNNTGG 1 cut(s) 345
Bsu15I ATCGAT 1 cut(s) 25
BsuRI GGCC 1 cut(s) 132
BsuTUI ATCGAT 1 cut(s) 25
BtgI CCRYGG 1 cut(s) 829
BtsCI GGATG 4 cut(s) 68, 297, 531, 565
BtsIMutI CAGTG 3 cut(s) 666, 747, 771
BveI ACCTGC 1 cut(s) 524
Cac8I GCNNGC 3 cut(s) 211, 810, 842
CciI TCATGA 1 cut(s) 378
CfoI GCGC 2 cut(s) 669, 846
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 2 cut(s) 188, 257
ClaI ATCGAT 1 cut(s) 25
Csp6I GTAC 4 cut(s) 88, 470, 611, 723
CviAII CATG 1 cut(s) 379
CviQI GTAC 4 cut(s) 88, 470, 611, 723
DinI GGCGCC 1 cut(s) 845
DpnI GATC 4 cut(s) 28, 216, 383, 855
DpnII GATC 4 cut(s) 26, 214, 381, 853
Ecl136II GAGCTC 1 cut(s) 308
Eco105I TACGTA 1 cut(s) 469
Eco130I CCWWGG 1 cut(s) 107
Eco24I GRGCYC 1 cut(s) 310
Eco31I GGTCTC 2 cut(s) 354, 402
Eco47I GGWCC 2 cut(s) 188, 257
Eco53kI GAGCTC 1 cut(s) 308
Eco72I CACGTG 1 cut(s) 419
EcoICRI GAGCTC 1 cut(s) 308
EcoRII CCWGG 2 cut(s) 10, 150
EcoT14I CCWWGG 1 cut(s) 107
EcoT38I GRGCYC 1 cut(s) 310
EgeI GGCGCC 1 cut(s) 845
EheI GGCGCC 1 cut(s) 845
ErhI CCWWGG 1 cut(s) 107
FaeI CATG 1 cut(s) 382
FaqI GGGAC 2 cut(s) 802, 847
FatI CATG 1 cut(s) 378
FauI CCCGC 1 cut(s) 779
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 6 cut(s) 78, 81, 330, 746, 770, 784
FokI GGATG 4 cut(s) 75, 304, 538, 552
FriOI GRGCYC 1 cut(s) 310
Fsp4HI GCNGC 6 cut(s) 78, 81, 330, 746, 770, 784
FspBI CTAG 1 cut(s) 726
GlaI GCGC 2 cut(s) 668, 845
GluI GCNGC 6 cut(s) 78, 81, 330, 746, 770, 784
HaeII RGCGCY 1 cut(s) 847
HaeIII GGCC 1 cut(s) 132
HapII CCGG 2 cut(s) 38, 681
HhaI GCGC 2 cut(s) 669, 846
Hin1I GRCGYC 1 cut(s) 844
Hin1II CATG 1 cut(s) 382
Hin6I GCGC 2 cut(s) 667, 844
HinP1I GCGC 2 cut(s) 667, 844
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HindIII AAGCTT 1 cut(s) 703
HinfI GANTC 5 cut(s) 57, 478, 598, 642, 711
HpaII CCGG 2 cut(s) 38, 681
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 3 cut(s) 6, 424, 613
Hpy188I TCNGA 3 cut(s) 62, 188, 695
Hpy188III TCNNGA 4 cut(s) 379, 401, 482, 595
Hpy8I GTNNAC 3 cut(s) 6, 424, 613
HpyAV CCTTC 2 cut(s) 208, 637
HpyCH4III ACNGT 2 cut(s) 261, 559
HpyCH4IV ACGT 4 cut(s) 50, 145, 418, 468
HpyCH4V TGCA 7 cut(s) 77, 245, 332, 533, 689, 745, 769
HpyF10VI GCNNNNNNNGC 2 cut(s) 329, 686
HpySE526I ACGT 4 cut(s) 50, 145, 418, 468
Hsp92I GRCGYC 1 cut(s) 844
Hsp92II CATG 1 cut(s) 382
HspAI GCGC 2 cut(s) 667, 844
KasI GGCGCC 1 cut(s) 843
Kzo9I GATC 4 cut(s) 26, 214, 381, 853
LmnI GCTCC 2 cut(s) 305, 664
Lsp1109I GCAGC 6 cut(s) 67, 89, 316, 757, 781, 795
LweI GCATC 1 cut(s) 282
MaeI CTAG 1 cut(s) 726
MaeII ACGT 4 cut(s) 50, 145, 418, 468
MaeIII GTNAC 1 cut(s) 166
MalI GATC 4 cut(s) 28, 216, 383, 855
MboI GATC 4 cut(s) 26, 214, 381, 853
MboII GAAGA 3 cut(s) 169, 452, 711
MhlI GDGCHC 1 cut(s) 310
MluCI AATT 5 cut(s) 172, 412, 584, 617, 652
Mly113I GGCGCC 1 cut(s) 844
MlyI GAGTC 4 cut(s) 66, 472, 592, 705
MmeI TCCRAC 2 cut(s) 40, 118
MnlI CCTC 6 cut(s) 153, 248, 579, 612, 689, 857
MseI TTAA 3 cut(s) 447, 554, 859
MslI CAYNNNNRTG 3 cut(s) 343, 740, 764
MspA1I CMGCKG 2 cut(s) 80, 786
MspI CCGG 2 cut(s) 38, 681
MspR9I CCNGG 2 cut(s) 12, 152
Mva1269I GAATGC 1 cut(s) 689
MvaI CCWGG 2 cut(s) 12, 152
MwoI GCNNNNNNNGC 2 cut(s) 329, 686
NarI GGCGCC 1 cut(s) 844
NdeII GATC 4 cut(s) 26, 214, 381, 853
NlaIII CATG 1 cut(s) 382
NlaIV GGNNCC 2 cut(s) 190, 845
OliI CACNNNNGTG 2 cut(s) 740, 764
PagI TCATGA 1 cut(s) 378
PctI GAATGC 1 cut(s) 689
PfeI GAWTC 1 cut(s) 642
PkrI GCNGC 6 cut(s) 79, 82, 331, 747, 771, 785
PleI GAGTC 4 cut(s) 65, 472, 592, 705
PluTI GGCGCC 1 cut(s) 847
PmaCI CACGTG 1 cut(s) 419
PmlI CACGTG 1 cut(s) 419
PpsI GAGTC 4 cut(s) 65, 472, 592, 705
Ppu21I YACGTR 2 cut(s) 419, 469
PsiI TTATAA 2 cut(s) 102, 221
Psp124BI GAGCTC 1 cut(s) 310
Psp1406I AACGTT 1 cut(s) 145
Psp6I CCWGG 2 cut(s) 10, 150
PspCI CACGTG 1 cut(s) 419
PspGI CCWGG 2 cut(s) 10, 150
PspN4I GGNNCC 2 cut(s) 190, 845
PspPI GGNCC 2 cut(s) 188, 257
PstI CTGCAG 1 cut(s) 334
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 4 cut(s) 89, 471, 612, 724
RsaNI GTAC 4 cut(s) 88, 470, 611, 723
RseI CAYNNNNRTG 3 cut(s) 343, 740, 764
SacI GAGCTC 1 cut(s) 310
SaqAI TTAA 3 cut(s) 447, 554, 859
SatI GCNGC 6 cut(s) 78, 81, 330, 746, 770, 784
Sau3AI GATC 4 cut(s) 26, 214, 381, 853
Sau96I GGNCC 2 cut(s) 188, 257
ScaI AGTACT 1 cut(s) 724
SchI GAGTC 4 cut(s) 66, 472, 592, 705
ScrFI CCNGG 2 cut(s) 12, 152
SduI GDGCHC 1 cut(s) 310
SfaNI GCATC 1 cut(s) 282
SfcI CTRYAG 1 cut(s) 330
SfoI GGCGCC 1 cut(s) 845
SgrAI CRCCGGYG 1 cut(s) 37
SinI GGWCC 2 cut(s) 188, 257
SmiMI CAYNNNNRTG 3 cut(s) 343, 740, 764
SmlI CTYRAG 1 cut(s) 593
SmoI CTYRAG 1 cut(s) 593
SnaBI TACGTA 1 cut(s) 469
Sse9I AATT 5 cut(s) 172, 412, 584, 617, 652
SsiI CCGC 2 cut(s) 35, 786
SspDI GGCGCC 1 cut(s) 843
SspMI CTAG 1 cut(s) 726
SstI GAGCTC 1 cut(s) 310
StyD4I CCNGG 2 cut(s) 10, 150
StyI CCWWGG 1 cut(s) 107
TaaI ACNGT 2 cut(s) 261, 559
TaiI ACGT 4 cut(s) 53, 148, 421, 471
TaqI TCGA 2 cut(s) 25, 709
TasI AATT 5 cut(s) 172, 412, 584, 617, 652
TatI WGTACW 2 cut(s) 87, 722
TfiI GAWTC 1 cut(s) 642
Tru1I TTAA 3 cut(s) 447, 554, 859
Tru9I TTAA 3 cut(s) 447, 554, 859
TscAI CASTG 3 cut(s) 666, 747, 771
TseI GCWGC 6 cut(s) 77, 80, 329, 745, 769, 783
TspDTI ATGAA 2 cut(s) 367, 554
TspGWI ACGGA 2 cut(s) 342, 818
TspRI CASTG 3 cut(s) 666, 747, 771
VpaK11BI GGWCC 2 cut(s) 188, 257
XapI RAATTY 2 cut(s) 412, 584
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 726
ZrmI AGTACT 1 cut(s) 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.