Rw2G006250

May be involved in the degradation of misfolded endoplasmic reticulum (ER) luminal proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004629
Physical Location & Seq
Forward (+)
6057383 .. 6060342
2960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006250.1

Sequence Viewer

Length: 750 bp
ATGTCTTCTCCTGCTGAATTCTATCAGTCACTCCCACCCATAAGCAAGGCTTACGGGACCATCTGTGTGGTGGCAACTGCGGCCTTTCAATTGGGACTTTATGATCCTCGAACTATTGCATTATTATATGGACCAGTATTTTCACAGTTCCAGGTGTGGAGGTTGATTACAAATTTCTTTTTCCTTGGGAAGTTCTCTGTCAACTTTGGAATTCGCCTTTTAATGATAGCAAGATATGGAGTTCAACTTGAAAAGGGACCATTTGAACGGCGTACTGCTGATTTCTTGTGGATGATGATATTTGGAGCCCTGACATTATTGGTTTTATCAGCCATCCCACTGTTTTGGTCTCCATTCTTGGGGGTATCTCTTGTGTTCATGCTTGTTTATGTCTGGAGTAGAGAATATCCGAATGCTAATATCAACTTATATGGCCTCGTGCAACTTAAGGCATTTTATTTCCCCTGGGCGATGCTTGCTTTGGACGTCATATTTGGTTCACCAATTTGGCCAGATCTGCTGGGAATCATTGCTGGACATCTGTACCACTTCTTGACCGTGTTGCATCCATTGGCAGGTGGAAAGAACATATTGAAGACTCCAAGATGGGTACATAAAATAGTTGCAAGATGGAGGATAGGTGCTCCTCCACCAACAAGCCGATCCCAACCTGCTGCTGCTGCCGCTGCCGCTGCCGGTACGGGTACGGGTGTGGCTTTCAGAGGGAGGTCTTATCGACTTAATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DER1

Protein Analysis

249

Amino Acids

27.81

Weight (kDa)

10.09

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DER1 PF04511 11 - 201 1e-45 Der1-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 566
AatII GACGTC 1 cut(s) 489
Acc36I ACCTGC 2 cut(s) 566, 679
AciI CCGC 3 cut(s) 80, 684, 690
AclWI GGATC 2 cut(s) 98, 657
AcoI YGGCCR 1 cut(s) 509
AcsI RAATTY 3 cut(s) 17, 172, 210
AcyI GRCGYC 1 cut(s) 486
AfaI GTAC 5 cut(s) 274, 545, 612, 700, 706
AfiI CCNNNNNNNGG 2 cut(s) 359, 575
AflII CTTAAG 1 cut(s) 446
AgsI TTSAA 5 cut(s) 89, 245, 251, 266, 595
AjnI CCWGG 2 cut(s) 150, 464
Alw21I GWGCWC 1 cut(s) 646
Alw26I GTCTC 1 cut(s) 354
AlwI GGATC 2 cut(s) 98, 657
AoxI GGCC 3 cut(s) 81, 433, 509
ApeKI GCWGC 5 cut(s) 674, 677, 680, 686, 692
ApoI RAATTY 3 cut(s) 17, 172, 210
ArsI GACNNNNNNTTYG 2 cut(s) 476, 508
AspS9I GGNCC 3 cut(s) 57, 131, 257
AsuHPI GGTGA 1 cut(s) 492
AvaII GGWCC 3 cut(s) 57, 131, 257
BaeI ACNNNNGTAYC 4 cut(s) 527, 560, 696, 729
BalI TGGCCA 1 cut(s) 511
BanII GRGCYC 1 cut(s) 310
BauI CACGAG 1 cut(s) 437
BbsI GAAGAC 1 cut(s) 602
Bbv12I GWGCWC 1 cut(s) 646
BbvI GCAGC 5 cut(s) 661, 664, 667, 673, 679
BccI CCATC 4 cut(s) 68, 341, 600, 624
BceAI ACGGC 1 cut(s) 284
BciT130I CCWGG 2 cut(s) 152, 466
BcoDI GTCTC 1 cut(s) 354
BfrI CTTAAG 1 cut(s) 446
BfuAI ACCTGC 2 cut(s) 566, 679
BglII AGATCT 1 cut(s) 514
BisI GCNGC 8 cut(s) 81, 675, 678, 681, 684, 687, 690, 693
BlsI GCNGC 8 cut(s) 82, 676, 679, 682, 685, 688, 691, 694
Bme1390I CCNGG 2 cut(s) 152, 466
Bme18I GGWCC 3 cut(s) 57, 131, 257
BmgT120I GGNCC 3 cut(s) 57, 131, 257
BmiI GGNNCC 3 cut(s) 58, 258, 307
BmrFI CCNGG 2 cut(s) 152, 466
BmsI GCATC 2 cut(s) 462, 574
BpiI GAAGAC 1 cut(s) 602
BpmI CTGGAG 1 cut(s) 415
BsaHI GRCGYC 1 cut(s) 486
BsaI GGTCTC 1 cut(s) 354
BsaJI CCNNGG 3 cut(s) 184, 464, 465
BsaXI ACNNNNNCTCC 2 cut(s) 625, 655
Bsc4I CCNNNNNNNGG 2 cut(s) 359, 575
Bse118I RCCGGY 1 cut(s) 695
Bse1I ACTGG 1 cut(s) 134
Bse3DI GCAATG 1 cut(s) 528
BseBI CCWGG 2 cut(s) 152, 466
BseDI CCNNGG 3 cut(s) 184, 464, 465
BseGI GGATG 3 cut(s) 297, 333, 565
BseLI CCNNNNNNNGG 2 cut(s) 359, 575
BseMI GCAATG 1 cut(s) 528
BseNI ACTGG 1 cut(s) 134
BseRI GAGGAG 1 cut(s) 636
BseXI GCAGC 5 cut(s) 661, 664, 667, 673, 679
BseYI CCCAGC 1 cut(s) 520
BshFI GGCC 3 cut(s) 83, 435, 511
BsiHKAI GWGCWC 1 cut(s) 646
BsiSI CCGG 1 cut(s) 696
BslFI GGGAC 3 cut(s) 70, 108, 270
BslI CCNNNNNNNGG 2 cut(s) 359, 575
BsmAI GTCTC 1 cut(s) 354
BsmFI GGGAC 3 cut(s) 70, 108, 270
BsmI GAATGC 1 cut(s) 418
BsnI GGCC 3 cut(s) 83, 435, 511
Bso31I GGTCTC 1 cut(s) 354
Bsp1286I GDGCHC 2 cut(s) 310, 646
Bsp143I GATC 3 cut(s) 103, 514, 662
BspACI CCGC 3 cut(s) 80, 684, 690
BspANI GGCC 3 cut(s) 83, 435, 511
BspLI GGNNCC 3 cut(s) 58, 258, 307
BspMI ACCTGC 2 cut(s) 566, 679
BspPI GGATC 2 cut(s) 98, 657
BspTI CTTAAG 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 354
BsrDI GCAATG 1 cut(s) 528
BsrFI RCCGGY 1 cut(s) 695
BsrI ACTGG 1 cut(s) 134
BssAI RCCGGY 1 cut(s) 695
BssECI CCNNGG 3 cut(s) 184, 464, 465
BssMI GATC 3 cut(s) 103, 514, 662
BssNI GRCGYC 1 cut(s) 486
BssSI CACGAG 1 cut(s) 437
BssT1I CCWWGG 1 cut(s) 184
Bst2BI CACGAG 1 cut(s) 437
Bst2UI CCWGG 2 cut(s) 152, 466
Bst4CI ACNGT 3 cut(s) 147, 342, 559
BstACI GRCGYC 1 cut(s) 486
BstAFI CTTAAG 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 477
BstF5I GGATG 3 cut(s) 297, 333, 565
BstKTI GATC 3 cut(s) 106, 517, 665
BstMAI GTCTC 1 cut(s) 354
BstMBI GATC 3 cut(s) 103, 514, 662
BstMWI GCNNNNNNNGC 8 cut(s) 80, 476, 517, 680, 683, 686, 689, 692
BstNI CCWGG 2 cut(s) 152, 466
BstSCI CCNGG 2 cut(s) 150, 464
BstV1I GCAGC 5 cut(s) 661, 664, 667, 673, 679
BstV2I GAAGAC 1 cut(s) 602
BstX2I RGATCY 1 cut(s) 514
BstXI CCANNNNNNTGG 2 cut(s) 67, 345
BstYI RGATCY 1 cut(s) 514
BsuRI GGCC 3 cut(s) 83, 435, 511
BtgZI GCGATG 1 cut(s) 485
BtsCI GGATG 3 cut(s) 297, 333, 565
BtsIMutI CAGTG 1 cut(s) 338
BveI ACCTGC 2 cut(s) 566, 679
Cac8I GCNNGC 1 cut(s) 477
Cfr10I RCCGGY 1 cut(s) 695
Cfr13I GGNCC 3 cut(s) 57, 131, 257
Csp6I GTAC 5 cut(s) 273, 544, 611, 699, 705
CviAII CATG 1 cut(s) 379
CviJI RGCY 8 cut(s) 50, 83, 308, 332, 435, 511, 660, 716
CviKI_1 RGCY 8 cut(s) 50, 83, 308, 332, 435, 511, 660, 716
CviQI GTAC 5 cut(s) 273, 544, 611, 699, 705
DpnI GATC 3 cut(s) 105, 516, 664
DpnII GATC 3 cut(s) 103, 514, 662
EaeI YGGCCR 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 310
Eco31I GGTCTC 1 cut(s) 354
Eco47I GGWCC 3 cut(s) 57, 131, 257
EcoRI GAATTC 2 cut(s) 17, 210
EcoRII CCWGG 2 cut(s) 150, 464
EcoT14I CCWWGG 1 cut(s) 184
EcoT38I GRGCYC 1 cut(s) 310
ErhI CCWWGG 1 cut(s) 184
FaeI CATG 1 cut(s) 382
FalI AAGNNNNNCTT 2 cut(s) 34, 66
FaqI GGGAC 3 cut(s) 70, 108, 270
FatI CATG 1 cut(s) 378
Fnu4HI GCNGC 8 cut(s) 81, 675, 678, 681, 684, 687, 690, 693
FokI GGATG 3 cut(s) 304, 320, 552
FriOI GRGCYC 1 cut(s) 310
Fsp4HI GCNGC 8 cut(s) 81, 675, 678, 681, 684, 687, 690, 693
GluI GCNGC 8 cut(s) 81, 675, 678, 681, 684, 687, 690, 693
GsaI CCCAGC 1 cut(s) 524
GsuI CTGGAG 1 cut(s) 415
HaeIII GGCC 3 cut(s) 83, 435, 511
HapII CCGG 1 cut(s) 696
Hin1I GRCGYC 1 cut(s) 486
Hin1II CATG 1 cut(s) 382
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 2 cut(s) 525, 598
HpaII CCGG 1 cut(s) 696
HphI GGTGA 1 cut(s) 492
Hpy166II GTNNAC 2 cut(s) 202, 500
Hpy188I TCNGA 2 cut(s) 411, 722
Hpy188III TCNNGA 2 cut(s) 394, 553
Hpy8I GTNNAC 2 cut(s) 202, 500
HpyCH4III ACNGT 3 cut(s) 147, 342, 559
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 4 cut(s) 119, 442, 565, 626
HpyF10VI GCNNNNNNNGC 8 cut(s) 80, 476, 517, 680, 683, 686, 689, 692
HpySE526I ACGT 1 cut(s) 486
Hsp92I GRCGYC 1 cut(s) 486
Hsp92II CATG 1 cut(s) 382
Kzo9I GATC 3 cut(s) 103, 514, 662
LmnI GCTCC 2 cut(s) 305, 649
Lsp1109I GCAGC 5 cut(s) 661, 664, 667, 673, 679
LweI GCATC 2 cut(s) 462, 574
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 1 cut(s) 27
MalI GATC 3 cut(s) 105, 516, 664
MboI GATC 3 cut(s) 103, 514, 662
MboII GAAGA 1 cut(s) 607
MfeI CAATTG 1 cut(s) 89
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 2 cut(s) 310, 646
MlsI TGGCCA 1 cut(s) 511
MluCI AATT 5 cut(s) 17, 89, 172, 210, 504
MluNI TGGCCA 1 cut(s) 511
MlyI GAGTC 1 cut(s) 592
MnlI CCTC 7 cut(s) 117, 153, 446, 627, 657, 716, 720
Mox20I TGGCCA 1 cut(s) 511
MscI TGGCCA 1 cut(s) 511
MseI TTAA 4 cut(s) 221, 447, 741, 748
MslI CAYNNNNRTG 1 cut(s) 65
Msp20I TGGCCA 1 cut(s) 511
MspA1I CMGCKG 2 cut(s) 686, 692
MspCI CTTAAG 1 cut(s) 446
MspI CCGG 1 cut(s) 696
MspR9I CCNGG 2 cut(s) 152, 466
MunI CAATTG 1 cut(s) 89
Mva1269I GAATGC 1 cut(s) 418
MvaI CCWGG 2 cut(s) 152, 466
MwoI GCNNNNNNNGC 8 cut(s) 80, 476, 517, 680, 683, 686, 689, 692
NdeII GATC 3 cut(s) 103, 514, 662
NlaIII CATG 1 cut(s) 382
NlaIV GGNNCC 3 cut(s) 58, 258, 307
NmuCI GTSAC 1 cut(s) 27
PaqCI CACCTGC 1 cut(s) 566
PasI CCCWGGG 1 cut(s) 465
PctI GAATGC 1 cut(s) 418
PfeI GAWTC 1 cut(s) 525
PkrI GCNGC 8 cut(s) 82, 676, 679, 682, 685, 688, 691, 694
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
Psp6I CCWGG 2 cut(s) 150, 464
PspFI CCCAGC 1 cut(s) 520
PspGI CCWGG 2 cut(s) 150, 464
PspN4I GGNNCC 3 cut(s) 58, 258, 307
PspPI GGNCC 3 cut(s) 57, 131, 257
PsuI RGATCY 1 cut(s) 514
RsaI GTAC 5 cut(s) 274, 545, 612, 700, 706
RsaNI GTAC 5 cut(s) 273, 544, 611, 699, 705
RseI CAYNNNNRTG 1 cut(s) 65
SaqAI TTAA 4 cut(s) 221, 447, 741, 748
SatI GCNGC 8 cut(s) 81, 675, 678, 681, 684, 687, 690, 693
Sau3AI GATC 3 cut(s) 103, 514, 662
Sau96I GGNCC 3 cut(s) 57, 131, 257
SchI GAGTC 1 cut(s) 592
ScrFI CCNGG 2 cut(s) 152, 466
SduI GDGCHC 2 cut(s) 310, 646
SetI ASST 7 cut(s) 156, 164, 489, 580, 643, 673, 731
SfaNI GCATC 2 cut(s) 462, 574
SinI GGWCC 3 cut(s) 57, 131, 257
SmiMI CAYNNNNRTG 1 cut(s) 65
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 5 cut(s) 17, 89, 172, 210, 504
SsiI CCGC 3 cut(s) 80, 684, 690
StyD4I CCNGG 2 cut(s) 150, 464
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 3 cut(s) 147, 342, 559
TaiI ACGT 1 cut(s) 489
TaqI TCGA 2 cut(s) 109, 736
TasI AATT 5 cut(s) 17, 89, 172, 210, 504
TauI GCSGC 3 cut(s) 83, 686, 692
TfiI GAWTC 1 cut(s) 525
Tru1I TTAA 4 cut(s) 221, 447, 741, 748
Tru9I TTAA 4 cut(s) 221, 447, 741, 748
TscAI CASTG 1 cut(s) 345
TseFI GTSAC 1 cut(s) 27
TseI GCWGC 5 cut(s) 674, 677, 680, 686, 692
Tsp45I GTSAC 1 cut(s) 27
TspDTI ATGAA 1 cut(s) 367
TspRI CASTG 1 cut(s) 345
Vha464I CTTAAG 1 cut(s) 446
VpaK11BI GGWCC 3 cut(s) 57, 131, 257
XapI RAATTY 3 cut(s) 17, 172, 210
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
ZraI GACGTC 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.