Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0002641

Physical Location & Seq

Genomic Coordinates Reverse (-)

3450584 .. 3454923

Gene Structure 4340 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g06540.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1944 bp

ATGACTTGCGATGGTAAACCAGAACTAAAAAAGAGATATTCCTTCTTGATATTTTTTCTTGTCATACAAGAAACTACAAAGGAAAGTTACACTTTATTCTCGACCCGGTTGAGTAAAACCCAACCGAAACCCAAAACGCGTACCCGACTCACCCAAGCTGCTAAGGTGGCATCACTTCCCCCGTCCACCTGTCCGTCCCTCCAGTATCTTCCAGTCAATCCGACGGCCCACAATCCCTCCCCTAGAAACTTCCACACGTGTCTTCCCTCCGCCTTTAGTGGCGGAGCTCACAGTCACAAAGTGACAAAACCAACGACTCTTCTTCCAAATCAGTCCACCGATTTCTCAGCCACTAACGGCGTCGCCGCTATGTTCAAGACCGCCACGTCACGCCTCCGGGCTCTCAAGGGAAGGACAGGCATTAGGGGACTCGCTAGGTTTTCGACATTGGGTGAAGTTTCGAGTCCAGCCGCGGTTGCGGCGAGGCCTTCTTGGTCTCCTTTGGGTGGTTTTTTTGGTTGGCTGACCGGAGGACGGTCCAGGTCTATGCCACCTCTGGATTTTCCACTTCAAGGTGTTAAGTTTCCACCTGCATTGCCGGATTATGTACAACCGGAGAAGCCAAAGATTACTACTCTGCCGAATGGTGTCAAAGTAGCTTCGATGAAGTCAGAGATACCTACAGCTTCCATAGGTTTATATATTAATTGTGGTTCGGTCTATGAGACACCAGAGTCATCCGGGGGCTCACACCTGCTGGAGCGTATGGCTTTCAAGAGCACAAGTAACCGCAGTCATTTGCGTATTGTGCGGGAGGTGGAGGCGATTGGTGGCACTGTGCAGGCGTCAGCTTCTCGGGAGCAGATGAGTTACACCATTAGTTCTCTGAAGACTTATGTTCCTGAAATGGTGGAGCTGCTTCTTGATTCAGTTCGAAATCCTGTTTTCCTTGATTGGGAAGTGAAGGAGGAACTTCTCAGGGTGAAAGACGATATTAATAAAGCCTCCAAAAACCCTGAAGCCCTTGTTTTGGAAGCAGTTCACTGTGTTGGTTACAAGGGTGGATTGGCAAATCCACTCTTGGCCCAAGAATCTTCGATTAGTAGCCTAAATAGTGAAACTTTGGAGGAATTCGTTACCGAAAATTATACTGCTCCTCGGATGGTGCTGGCCGCTTATGGTGTTGAACATGAGGAACTTCTAAAAATTGCAGAACCACTTTTGTCTGATCTCCCCAGTGTCCCTCTTGCTGAGGAGCCAACGTCTGTGTACACTGGAGGTGACTACCGTTGTCAAGCTGAATCAGGGGATACTCATTTTGCTCTTGCATTTGAACTTCCTGGTGGATGGCTTAGGATGAAGGATGCAATGATTTTGAATGTTCTCCAGATGCTAATGGGAGGAGGTGAATCCTTTTCAAGTGGTGGACCTGGAAAAGGGATGAACTCGCGATTATATATTCATGTGTTGGCTCAAAATCCTGAGGTTAAGTCAATTTCAGCAATCAATAGCATATACAACAACAGCGCTATATTCGGCATCCAAGCTTCCGCTAGTTCAGATTATGTGGCAGAAGCCATTGATATTGCGGCCGATGAGCTTGTTGCACTTGCAACTCCTGGAGAAGTTGACGTGGTACAGCTAGATCGTGCCAAACAGTCAACGAAGTCTTCCATTCTTATGCATATGGAATCTAGAATGGTTGTTGTAGACGATATTGGGAAACAAATCTTGACGTACAATGATTGGAAATTGGAGCAACTCATGAAGGCTGTAGATGAGGTTTCTATGGAAGATATTACTTCAACAACTCAAAAGCTTTTATCGACCCCTCTCACAATGGCATCATATGGAGATGTCACTAAAGTCCCACGCTATGATTTAGTCAGCCAAAAATTTTCTCTCCCAAATAAGAAAACAAAGTCAGCAAAGAAGATCACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0016020 GO:0019866 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M16 Peptidase_M16_C

Protein Analysis

648

Amino Acids

70.43

Weight (kDa)

8.29

Isoelectric Point (pI)

39.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	219 - 362	2.9e-40	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	369 - 553	8e-27	Peptidase M16 inactive domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002641)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51980 AT1G51980 AT3G16480
fragaria_vesca	FvH4_1g06540 FvH4_1g06540 FvH4_1g06540 FvH4_6g32600 FvH4_6g32600
malus_domestica	MD02G1068800.v1.1 MD09G1200100.v1.1 MD17G1181200.v1.1
prunus_persica	Prupe.3G056700_v2.0.a1 Prupe.7G216900_v2.0.a1
pyrus_communis	pycom02g05450 pycom09g11770 pycom17g19060
rosa_chinensis	RchiOBHm_Chr2g0092291 RchiOBHm_Chr2g0141051
rosa_laevigata	RLG00000016294 RLG00000019911
rosa_multiflora	Rmu_co8416335.1_g000001 Rmu_sc0003289.1_g000021 Rmu_sc0004769.1_g000017 Rmu_sc0012897.1_g000005
rosa_roxburghii	Rroxscaffold_2G00104250 Rroxscaffold_2G00149290
rosa_rugosa	Rorug02G0024700 Rorug02G0366400
rosa_samantha	Rh2AG070400 Rh2AG417800 Rh2BG069900 Rh2BG427700 Rh2CG071100 Rh2CG356200 Rh2CG404100 Rh2DG068900 Rh2DG437200
rosa_wichuraiana	Rw2G006190 Rw2G034200 Rw2G034260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	598, 762
AasI	GACNNNNNNGTC	2 cut(s)	385, 733
Acc36I	ACCTGC	2 cut(s)	598, 762
AccI	GTMKAC	1 cut(s)	1710
AccII	CGCG	3 cut(s)	139, 473, 1450
AcoI	YGGCCR	2 cut(s)	1170, 1590
AcsI	RAATTY	2 cut(s)	1130, 1895
AcuI	CTGAAG	2 cut(s)	908, 1038
AcvI	CACGTG	1 cut(s)	258
AcyI	GRCGYC	2 cut(s)	360, 845
AdeI	CACNNNGTG	1 cut(s)	301
AfaI	GTAC	5 cut(s)	142, 609, 1271, 1638, 1739
AfeI	AGCGCT	1 cut(s)	1528
AfiI	CCNNNNNNNGG	6 cut(s)	506, 534, 572, 955, 1030, 1436
AflIII	ACRYGT	3 cut(s)	137, 255, 257
AgsI	TTSAA	8 cut(s)	376, 572, 775, 1187, 1334, 1378, 1419, 1806
AjiI	CACGTC	2 cut(s)	387, 1633
AjnI	CCWGG	4 cut(s)	539, 1339, 1429, 1618
Alw21I	GWGCWC	2 cut(s)	289, 782
Alw26I	GTCTC	2 cut(s)	501, 719
Ama87I	CYCGRG	1 cut(s)	855
Aor51HI	AGCGCT	1 cut(s)	1528
AoxI	GGCC	5 cut(s)	225, 485, 1083, 1170, 1590
ApeKI	GCWGC	2 cut(s)	158, 916
ApoI	RAATTY	2 cut(s)	1130, 1895
AseI	ATTAAT	2 cut(s)	705, 996
Asp700I	GAANNNNTTC	1 cut(s)	1038
AspLEI	GCGC	1 cut(s)	1529
AspS9I	GGNCC	4 cut(s)	226, 537, 1084, 1427
AsuC2I	CCSGG	3 cut(s)	106, 398, 742
AsuHPI	GGTGA	5 cut(s)	142, 464, 994, 1292, 1418
AsuII	TTCGAA	1 cut(s)	934
AvaI	CYCGRG	1 cut(s)	855
AvaII	GGWCC	2 cut(s)	537, 1427
AxyI	CCTNAGG	1 cut(s)	1482
BanII	GRGCYC	3 cut(s)	289, 403, 749
BbrPI	CACGTG	1 cut(s)	258
BbsI	GAAGAC	3 cut(s)	254, 896, 1662
Bbv12I	GWGCWC	2 cut(s)	289, 782
BbvCI	CCTCAGC	1 cut(s)	1251
BbvI	GCAGC	2 cut(s)	145, 903
BccI	CCATC	3 cut(s)	5, 1156, 1341
BceAI	ACGGC	2 cut(s)	240, 373
BciT130I	CCWGG	4 cut(s)	541, 1341, 1431, 1620
BciVI	GTATCC	1 cut(s)	1303
BcnI	CCSGG	3 cut(s)	106, 398, 742
BcoDI	GTCTC	2 cut(s)	501, 719
BfaI	CTAG	5 cut(s)	243, 435, 1554, 1643, 1695
BfmI	CTRYAG	2 cut(s)	681, 1773
BfoI	RGCGCY	1 cut(s)	1530
BfuAI	ACCTGC	2 cut(s)	598, 762
BfuI	GTATCC	1 cut(s)	1303
BisI	GCNGC	7 cut(s)	159, 366, 471, 480, 917, 1173, 1590
BlsI	GCNGC	7 cut(s)	160, 367, 472, 481, 918, 1174, 1591
Bme1390I	CCNGG	7 cut(s)	106, 398, 541, 742, 1341, 1431, 1620
Bme18I	GGWCC	2 cut(s)	537, 1427
BmeT110I	CYCGRG	1 cut(s)	855
BmgBI	CACGTC	2 cut(s)	387, 1633
BmgT120I	GGNCC	4 cut(s)	226, 537, 1084, 1427
BmiI	GGNNCC	1 cut(s)	1257
BmrFI	CCNGG	7 cut(s)	106, 398, 541, 742, 1341, 1431, 1620
BmrI	ACTGGG	1 cut(s)	1230
BmsI	GCATC	5 cut(s)	179, 1354, 1380, 1548, 1853
BmuI	ACTGGG	1 cut(s)	1230
BpiI	GAAGAC	3 cut(s)	254, 896, 1662
BpmI	CTGGAG	5 cut(s)	185, 779, 1296, 1370, 1641
Bpu10I	CCTNAGC	3 cut(s)	162, 1251, 1352
Bpu14I	TTCGAA	1 cut(s)	934
BpuEI	CTTGAG	1 cut(s)	389
BpuMI	CCSGG	3 cut(s)	106, 398, 742
BsaAI	YACGTR	1 cut(s)	258
BsaHI	GRCGYC	2 cut(s)	360, 845
BsaI	GGTCTC	1 cut(s)	501
BsaJI	CCNNGG	3 cut(s)	471, 741, 1157
BsaWI	WCCGGW	2 cut(s)	527, 613
BsaXI	ACNNNNNCTCC	4 cut(s)	221, 251, 1247, 1277
Bsc4I	CCNNNNNNNGG	6 cut(s)	506, 534, 572, 955, 1030, 1436
Bse1I	ACTGG	4 cut(s)	202, 212, 1236, 1279
Bse21I	CCTNAGG	1 cut(s)	1482
Bse3DI	GCAATG	2 cut(s)	593, 1374
BseBI	CCWGG	4 cut(s)	541, 1341, 1431, 1620
BseDI	CCNNGG	3 cut(s)	471, 741, 1157
BseGI	GGATG	7 cut(s)	737, 1167, 1352, 1362, 1369, 1446, 1539
BseLI	CCNNNNNNNGG	6 cut(s)	506, 534, 572, 955, 1030, 1436
BseMI	GCAATG	2 cut(s)	593, 1374
BseMII	CTCAG	4 cut(s)	360, 991, 1242, 1473
BseNI	ACTGG	4 cut(s)	202, 212, 1236, 1279
BseRI	GAGGAG	3 cut(s)	1146, 1268, 1416
BseX3I	CGGCCG	1 cut(s)	1590
BseXI	GCAGC	2 cut(s)	145, 903
BsgI	GTGCAG	1 cut(s)	860
Bsh1236I	CGCG	3 cut(s)	139, 473, 1450
Bsh1285I	CGRYCG	1 cut(s)	1593
BshFI	GGCC	5 cut(s)	227, 487, 1085, 1172, 1592
BsiEI	CGRYCG	1 cut(s)	1593
BsiHKAI	GWGCWC	2 cut(s)	289, 782
BsiHKCI	CYCGRG	1 cut(s)	855
BsiSI	CCGG	6 cut(s)	106, 397, 528, 599, 614, 741
BslFI	GGGAC	4 cut(s)	181, 441, 1226, 1853
BslI	CCNNNNNNNGG	6 cut(s)	506, 534, 572, 955, 1030, 1436
BsmAI	GTCTC	2 cut(s)	501, 719
BsmFI	GGGAC	4 cut(s)	181, 441, 1226, 1853
BsnI	GGCC	5 cut(s)	227, 487, 1085, 1172, 1592
Bso31I	GGTCTC	1 cut(s)	501
BsoBI	CYCGRG	1 cut(s)	855
Bsp119I	TTCGAA	1 cut(s)	934
Bsp1286I	GDGCHC	4 cut(s)	289, 403, 749, 782
Bsp1407I	TGTACA	2 cut(s)	607, 1269
Bsp143I	GATC	3 cut(s)	1228, 1645, 1935
Bsp68I	TCGCGA	1 cut(s)	1450
BspANI	GGCC	5 cut(s)	227, 487, 1085, 1172, 1592
BspCNI	CTCAG	4 cut(s)	359, 990, 1243, 1474
BspFNI	CGCG	3 cut(s)	139, 473, 1450
BspHI	TCATGA	1 cut(s)	1764
BspLI	GGNNCC	1 cut(s)	1257
BspMI	ACCTGC	2 cut(s)	598, 762
BspT104I	TTCGAA	1 cut(s)	934
BspTNI	GGTCTC	1 cut(s)	501
BsrDI	GCAATG	2 cut(s)	593, 1374
BsrGI	TGTACA	2 cut(s)	607, 1269
BsrI	ACTGG	4 cut(s)	202, 212, 1236, 1279
BssECI	CCNNGG	3 cut(s)	471, 741, 1157
BssMI	GATC	3 cut(s)	1228, 1645, 1935
BssNI	GRCGYC	2 cut(s)	360, 845
Bst2UI	CCWGG	4 cut(s)	541, 1341, 1431, 1620
Bst4CI	ACNGT	6 cut(s)	293, 537, 838, 1046, 1289, 1659
Bst6I	CTCTTC	1 cut(s)	324
BstACI	GRCGYC	2 cut(s)	360, 845
BstAUI	TGTACA	2 cut(s)	607, 1269
BstBAI	YACGTR	1 cut(s)	258
BstBI	TTCGAA	1 cut(s)	934
BstC8I	GCNNGC	2 cut(s)	843, 1170
BstDEI	CTNAG	6 cut(s)	162, 346, 977, 1251, 1352, 1482
BstDSI	CCRYGG	1 cut(s)	471
BstENI	CCTNNNNNAGG	1 cut(s)	1434
BstF5I	GGATG	7 cut(s)	737, 1167, 1352, 1362, 1369, 1446, 1539
BstFNI	CGCG	3 cut(s)	139, 473, 1450
BstH2I	RGCGCY	1 cut(s)	1530
BstHHI	GCGC	1 cut(s)	1529
BstKTI	GATC	3 cut(s)	1231, 1648, 1938
BstMAI	GTCTC	2 cut(s)	501, 719
BstMBI	GATC	3 cut(s)	1228, 1645, 1935
BstMCI	CGRYCG	1 cut(s)	1593
BstMWI	GCNNNNNNNGC	4 cut(s)	167, 476, 479, 808
BstNI	CCWGG	4 cut(s)	541, 1341, 1431, 1620
BstSCI	CCNGG	7 cut(s)	104, 396, 539, 740, 1339, 1429, 1618
BstSFI	CTRYAG	2 cut(s)	681, 1773
BstUI	CGCG	3 cut(s)	139, 473, 1450
BstV1I	GCAGC	2 cut(s)	145, 903
BstV2I	GAAGAC	3 cut(s)	254, 896, 1662
BstZI	CGGCCG	1 cut(s)	1590
Bsu36I	CCTNAGG	1 cut(s)	1482
BsuI	GTATCC	1 cut(s)	1303
BsuRI	GGCC	5 cut(s)	227, 487, 1085, 1172, 1592
BtgI	CCRYGG	1 cut(s)	471
BtgZI	GCGATG	1 cut(s)	24
BtrI	CACGTC	2 cut(s)	387, 1633
BtsCI	GGATG	7 cut(s)	737, 1167, 1352, 1362, 1369, 1446, 1539
BtsIMutI	CAGTG	4 cut(s)	834, 1042, 1243, 1272
BtuMI	TCGCGA	1 cut(s)	1450
BveI	ACCTGC	2 cut(s)	598, 762
Cac8I	GCNNGC	2 cut(s)	843, 1170
CciI	TCATGA	1 cut(s)	1764
CfoI	GCGC	1 cut(s)	1529
Cfr13I	GGNCC	4 cut(s)	226, 537, 1084, 1427
Cfr42I	CCGCGG	1 cut(s)	474
CseI	GACGC	2 cut(s)	349, 834
Csp6I	GTAC	5 cut(s)	141, 608, 1270, 1637, 1738
CspCI	CAANNNNNGTGG	2 cut(s)	576, 611
CviAII	CATG	3 cut(s)	1190, 1463, 1765
CviQI	GTAC	5 cut(s)	141, 608, 1270, 1637, 1738
DdeI	CTNAG	6 cut(s)	162, 346, 977, 1251, 1352, 1482
DpnI	GATC	3 cut(s)	1230, 1647, 1937
DpnII	GATC	3 cut(s)	1228, 1645, 1935
DraIII	CACNNNGTG	1 cut(s)	301
DrdI	GACNNNNNNGTC	2 cut(s)	385, 733
DseDI	GACNNNNNNGTC	2 cut(s)	385, 733
EaeI	YGGCCR	2 cut(s)	1170, 1590
EagI	CGGCCG	1 cut(s)	1590
Eam1104I	CTCTTC	1 cut(s)	324
EarI	CTCTTC	1 cut(s)	324
EciI	GGCGGA	2 cut(s)	259, 297
Ecl136II	GAGCTC	1 cut(s)	287
EclXI	CGGCCG	1 cut(s)	1590
Eco147I	AGGCCT	1 cut(s)	487
Eco24I	GRGCYC	3 cut(s)	289, 403, 749
Eco31I	GGTCTC	1 cut(s)	501
Eco47I	GGWCC	2 cut(s)	537, 1427
Eco47III	AGCGCT	1 cut(s)	1528
Eco52I	CGGCCG	1 cut(s)	1590
Eco53kI	GAGCTC	1 cut(s)	287
Eco57I	CTGAAG	2 cut(s)	908, 1038
Eco72I	CACGTG	1 cut(s)	258
Eco81I	CCTNAGG	1 cut(s)	1482
Eco88I	CYCGRG	1 cut(s)	855
EcoICRI	GAGCTC	1 cut(s)	287
EcoNI	CCTNNNNNAGG	1 cut(s)	1434
EcoRI	GAATTC	1 cut(s)	1130
EcoRII	CCWGG	4 cut(s)	539, 1339, 1429, 1618
EcoT22I	ATGCAT	1 cut(s)	1686
EcoT38I	GRGCYC	3 cut(s)	289, 403, 749
FaeI	CATG	3 cut(s)	1193, 1466, 1768
FalI	AAGNNNNNCTT	2 cut(s)	76, 108
FaqI	GGGAC	4 cut(s)	181, 441, 1226, 1853
FatI	CATG	3 cut(s)	1189, 1462, 1764
FauI	CCCGC	1 cut(s)	804
FauNDI	CATATG	2 cut(s)	1686, 1849
FblI	GTMKAC	1 cut(s)	1710
Fnu4HI	GCNGC	7 cut(s)	159, 366, 471, 480, 917, 1173, 1590
FokI	GGATG	7 cut(s)	724, 1174, 1359, 1369, 1376, 1453, 1526
FriOI	GRGCYC	3 cut(s)	289, 403, 749
Fsp4HI	GCNGC	7 cut(s)	159, 366, 471, 480, 917, 1173, 1590
FspBI	CTAG	5 cut(s)	243, 435, 1554, 1643, 1695
GlaI	GCGC	1 cut(s)	1528
GluI	GCNGC	7 cut(s)	159, 366, 471, 480, 917, 1173, 1590
GsuI	CTGGAG	5 cut(s)	185, 779, 1296, 1370, 1641
HaeII	RGCGCY	1 cut(s)	1530
HaeIII	GGCC	5 cut(s)	227, 487, 1085, 1172, 1592
HapII	CCGG	6 cut(s)	106, 397, 528, 599, 614, 741
HgaI	GACGC	2 cut(s)	349, 834
HhaI	GCGC	1 cut(s)	1529
Hin1I	GRCGYC	2 cut(s)	360, 845
Hin1II	CATG	3 cut(s)	1193, 1466, 1768
Hin6I	GCGC	1 cut(s)	1527
HinP1I	GCGC	1 cut(s)	1527
HincII	GTYRAC	2 cut(s)	1630, 1662
HindII	GTYRAC	2 cut(s)	1630, 1662
HindIII	AAGCTT	2 cut(s)	1545, 1817
HpaII	CCGG	6 cut(s)	106, 397, 528, 599, 614, 741
HphI	GGTGA	5 cut(s)	142, 464, 994, 1292, 1418
Hpy188I	TCNGA	6 cut(s)	222, 673, 888, 1161, 1228, 1561
Hpy99I	CGWCG	2 cut(s)	226, 365
HpyAV	CCTTC	6 cut(s)	52, 405, 498, 958, 1354, 1762
HpyCH4III	ACNGT	6 cut(s)	293, 537, 838, 1046, 1289, 1659
HpyCH4IV	ACGT	5 cut(s)	257, 386, 1262, 1632, 1736
HpyCH4V	TGCA	8 cut(s)	593, 841, 1211, 1328, 1367, 1607, 1613, 1684
HpyF10VI	GCNNNNNNNGC	4 cut(s)	167, 476, 479, 808
HpyF3I	CTNAG	6 cut(s)	162, 346, 977, 1251, 1352, 1482
HpySE526I	ACGT	5 cut(s)	257, 386, 1262, 1632, 1736
Hsp92I	GRCGYC	2 cut(s)	360, 845
Hsp92II	CATG	3 cut(s)	1193, 1466, 1768
HspAI	GCGC	1 cut(s)	1527
KspI	CCGCGG	1 cut(s)	474
Kzo9I	GATC	3 cut(s)	1228, 1645, 1935
LmnI	GCTCC	7 cut(s)	284, 760, 859, 913, 1159, 1255, 1756
Lsp1109I	GCAGC	2 cut(s)	145, 903
LweI	GCATC	5 cut(s)	179, 1354, 1380, 1548, 1853
MaeI	CTAG	5 cut(s)	243, 435, 1554, 1643, 1695
MaeII	ACGT	5 cut(s)	257, 386, 1262, 1632, 1736
MalI	GATC	3 cut(s)	1230, 1647, 1937
MboI	GATC	3 cut(s)	1228, 1645, 1935
MboII	GAAGA	8 cut(s)	200, 254, 311, 314, 901, 1086, 1662, 1805
MhlI	GDGCHC	4 cut(s)	289, 403, 749, 782
MluCI	AATT	7 cut(s)	706, 1130, 1144, 1206, 1494, 1751, 1895
MluI	ACGCGT	1 cut(s)	137
MlyI	GAGTC	5 cut(s)	141, 310, 423, 472, 743
MmeI	TCCRAC	1 cut(s)	245
Mph1103I	ATGCAT	1 cut(s)	1686
MroXI	GAANNNNTTC	1 cut(s)	1038
MseI	TTAA	5 cut(s)	579, 705, 996, 1488, 1942
MslI	CAYNNNNRTG	1 cut(s)	1679
MspA1I	CMGCKG	1 cut(s)	473
MspI	CCGG	6 cut(s)	106, 397, 528, 599, 614, 741
MspR9I	CCNGG	7 cut(s)	106, 398, 541, 742, 1341, 1431, 1620
MvaI	CCWGG	4 cut(s)	541, 1341, 1431, 1620
MvnI	CGCG	3 cut(s)	139, 473, 1450
MwoI	GCNNNNNNNGC	4 cut(s)	167, 476, 479, 808
NciI	CCSGG	3 cut(s)	106, 398, 742
NdeI	CATATG	2 cut(s)	1686, 1849
NdeII	GATC	3 cut(s)	1228, 1645, 1935
NlaIII	CATG	3 cut(s)	1193, 1466, 1768
NlaIV	GGNNCC	1 cut(s)	1257
NmuCI	GTSAC	5 cut(s)	293, 301, 387, 1280, 1858
NruI	TCGCGA	1 cut(s)	1450
NsiI	ATGCAT	1 cut(s)	1686
NspV	TTCGAA	1 cut(s)	934
PagI	TCATGA	1 cut(s)	1764
PaqCI	CACCTGC	2 cut(s)	598, 762
PceI	AGGCCT	1 cut(s)	487
PdmI	GAANNNNTTC	1 cut(s)	1038
PfeI	GAWTC	5 cut(s)	926, 1091, 1301, 1409, 1691
PfoI	TCCNGGA	1 cut(s)	1618
PkrI	GCNGC	7 cut(s)	160, 367, 472, 481, 918, 1174, 1591
PleI	GAGTC	5 cut(s)	141, 310, 423, 471, 742
PmaCI	CACGTG	1 cut(s)	258
PmlI	CACGTG	1 cut(s)	258
PpsI	GAGTC	5 cut(s)	141, 310, 423, 471, 742
Ppu21I	YACGTR	1 cut(s)	258
PshBI	ATTAAT	2 cut(s)	705, 996
Psp124BI	GAGCTC	1 cut(s)	289
Psp6I	CCWGG	4 cut(s)	539, 1339, 1429, 1618
PspCI	CACGTG	1 cut(s)	258
PspGI	CCWGG	4 cut(s)	539, 1339, 1429, 1618
PspN4I	GGNNCC	1 cut(s)	1257
PspPI	GGNCC	4 cut(s)	226, 537, 1084, 1427
RruI	TCGCGA	1 cut(s)	1450
RsaI	GTAC	5 cut(s)	142, 609, 1271, 1638, 1739
RsaNI	GTAC	5 cut(s)	141, 608, 1270, 1637, 1738
RseI	CAYNNNNRTG	1 cut(s)	1679
SacI	GAGCTC	1 cut(s)	289
SacII	CCGCGG	1 cut(s)	474
SaqAI	TTAA	5 cut(s)	579, 705, 996, 1488, 1942
SatI	GCNGC	7 cut(s)	159, 366, 471, 480, 917, 1173, 1590
Sau3AI	GATC	3 cut(s)	1228, 1645, 1935
Sau96I	GGNCC	4 cut(s)	226, 537, 1084, 1427
SchI	GAGTC	5 cut(s)	141, 310, 423, 472, 743
ScrFI	CCNGG	7 cut(s)	106, 398, 541, 742, 1341, 1431, 1620
SduI	GDGCHC	4 cut(s)	289, 403, 749, 782
SfaNI	GCATC	5 cut(s)	179, 1354, 1380, 1548, 1853
SfcI	CTRYAG	2 cut(s)	681, 1773
Sfr303I	CCGCGG	1 cut(s)	474
SfuI	TTCGAA	1 cut(s)	934
SgrBI	CCGCGG	1 cut(s)	474
SinI	GGWCC	2 cut(s)	537, 1427
SmiMI	CAYNNNNRTG	1 cut(s)	1679
SmlI	CTYRAG	1 cut(s)	404
SmoI	CTYRAG	1 cut(s)	404
Sse9I	AATT	7 cut(s)	706, 1130, 1144, 1206, 1494, 1751, 1895
SseBI	AGGCCT	1 cut(s)	487
SspMI	CTAG	5 cut(s)	243, 435, 1554, 1643, 1695
SstI	GAGCTC	1 cut(s)	289
StuI	AGGCCT	1 cut(s)	487
StyD4I	CCNGG	7 cut(s)	104, 396, 539, 740, 1339, 1429, 1618
TaaI	ACNGT	6 cut(s)	293, 537, 838, 1046, 1289, 1659
TaiI	ACGT	5 cut(s)	260, 389, 1265, 1635, 1739
TaqI	TCGA	7 cut(s)	101, 443, 461, 662, 934, 1097, 1826
TaqII	GACCGA	1 cut(s)	706
TasI	AATT	7 cut(s)	706, 1130, 1144, 1206, 1494, 1751, 1895
TatI	WGTACW	2 cut(s)	607, 1269
TauI	GCSGC	5 cut(s)	368, 473, 482, 1175, 1592
TfiI	GAWTC	5 cut(s)	926, 1091, 1301, 1409, 1691
Tru1I	TTAA	5 cut(s)	579, 705, 996, 1488, 1942
Tru9I	TTAA	5 cut(s)	579, 705, 996, 1488, 1942
TscAI	CASTG	4 cut(s)	841, 1049, 1243, 1279
TseFI	GTSAC	5 cut(s)	293, 301, 387, 1280, 1858
TseI	GCWGC	2 cut(s)	158, 916
Tsp45I	GTSAC	5 cut(s)	293, 301, 387, 1280, 1858
TspDTI	ATGAA	5 cut(s)	680, 1373, 1451, 1457, 1781
TspGWI	ACGGA	1 cut(s)	183
TspRI	CASTG	4 cut(s)	841, 1049, 1243, 1279
VpaK11BI	GGWCC	2 cut(s)	537, 1427
VspI	ATTAAT	2 cut(s)	705, 996
XagI	CCTNNNNNAGG	1 cut(s)	1434
XapI	RAATTY	2 cut(s)	1130, 1895
XbaI	TCTAGA	1 cut(s)	1694
XmiI	GTMKAC	1 cut(s)	1710
XmnI	GAANNNNTTC	1 cut(s)	1038
XspI	CTAG	5 cut(s)	243, 435, 1554, 1643, 1695
Zsp2I	ATGCAT	1 cut(s)	1686

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.