Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0002641

Physical Location & Seq

Genomic Coordinates Forward (+)

21107010 .. 21111591

Gene Structure 4582 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1181200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGTACAAAGCAGCCTCCCGACTCAGGTCCCTCAAGGGCCGTGGAACTTTGGGGGCCACCCGGTTTGCAACAAGTGCAGCTGTATCGAAGCCTTCATCTGGGGGTCTCTTTAGCTGGCTGACGGGGGAGAGCTCAAGTGCCCTTCCTCCTTTGGAAACCCAACTCCCAGGTGTTAACCTTCCTCCTCCAGTACCTGATTATGTTGAACCAAGCAAGACCCAGATTACAACTCTTTCTAATGGTGTCAAAATAGCTTCAGAAACTTCAACGAGTCCTGCAGCGTCCATTGGAATATATGTGGATTCTGGTTCCATATATGAGACACCAGTATCCAGTGGTGCCTCACACTTGCTAGAACGATTGGCTTTTAAGAGTACAACCAACCGGAGCCACTTACGCATTGTGAGAGAAGTGGAAGCAATCGGTGGTCACATAGGAGCCAGTGCCTCTAGGGAGCAAATGGGATATACTTTTGATGCTATCAAGACCTATGTTCCCCAGATGGTGGAACTACTTGTTGACTCTTTGAGGAACCCTGCCTTCTTGGAATGGGAAGTCAATGAAGAGCTCAATAAGGTGCAGGCAGAGATTGGAGAACTTTCTAAGAATCCGCAGGGCCTACTCTTGGAGGCGATTCATTCTGCTGGTTATTCTGGTGCATTGGCTAATCCTCTATTGGCTTCTGAATCATCACTAAACAGATTGAATGGCAGCATCTTGGAGCAGTTCATTGCTGAAAATTATACTGCTCCTCGTATAGTTCTTGCAGCATCTGGGGTTGATCATCAGGAGATTGTATCCATTGCAGAACCACTTCTCTCTGACCTACCGAGTGTGCCTCGCTTCGAGGAGCCACTATCCAAATATGTTGGAGGAGAGTTCCGTTGTCAAGCTGATACACCGGAAACACACGTAGCTCTTGCATTTGAGGTTCCCGGTGGCTGGCGGCAAGAGAAGCAAGCCGTCCTTTTGACAGTTCTCCAGTTGCTGATGGGAGGTGGTGGCTCTTTCTCTGCTGGGGGCCCTGGAAAGGGGATGCACTCAAGGCTATATCTCCGCGTCTTGAATCAGTATCCACAGATCCAATCCTTTTCTGCATTCAACAGCATATTCAATGATTCTGGGTTGTTTGGTATTTGTGCTAGCACTGGCTCTGAATTTGCATCGAAAGCAGTTGATATAGCAGCACAGGAATTACTTACAATTGCAACACCTGGACAAGTTACGGATGTACAGCTCAAACGTGCCAAGGAGTCGACAAAGGCTGCAGTTTTAATGAATCTAGAATCTAGAATGATTGCATCTGAGGATATAGGAAGGCAGGTTTTGACATACGGAGAGAGGAAACCTGTGGAGCACTTCTTGAAGACAGTGGAAGGAGTGACGTTGAAAGACATTACTACAATCGCGCAGAAGATTATCTCTTCACCTCTAACACTGGCAACATATGGCAATGTTGTACATGTACCGGGTTACGACTCAATTAGCAGCAGGTTCCATGCAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006508 GO:0006605 GO:0006626 GO:0006627 GO:0006807 GO:0006810 GO:0006839 GO:0006886 GO:0006950 GO:0006970 GO:0006996 GO:0007005 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010467 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016485 GO:0016787 GO:0017076 GO:0019538 GO:0019866 GO:0019867 GO:0030554 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0034982 GO:0035639 GO:0036094 GO:0042221 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045184 GO:0045275 GO:0046872 GO:0046907 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0051604 GO:0051641 GO:0051649 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0070585 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072655 GO:0097159 GO:0097367 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M16 Peptidase_M16_C

Protein Analysis

503

Amino Acids

53.73

Weight (kDa)

5.99

Isoelectric Point (pI)

37.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	84 - 227	1.2e-40	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	240 - 418	6.8e-30	Peptidase M16 inactive domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002641)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51980 AT1G51980 AT3G16480
fragaria_vesca	FvH4_1g06540 FvH4_1g06540 FvH4_1g06540 FvH4_6g32600 FvH4_6g32600
malus_domestica	MD02G1068800.v1.1 MD09G1200100.v1.1 MD17G1181200.v1.1
prunus_persica	Prupe.3G056700_v2.0.a1 Prupe.7G216900_v2.0.a1
pyrus_communis	pycom02g05450 pycom09g11770 pycom17g19060
rosa_chinensis	RchiOBHm_Chr2g0092291 RchiOBHm_Chr2g0141051
rosa_laevigata	RLG00000016294 RLG00000019911
rosa_multiflora	Rmu_co8416335.1_g000001 Rmu_sc0003289.1_g000021 Rmu_sc0004769.1_g000017 Rmu_sc0012897.1_g000005
rosa_roxburghii	Rroxscaffold_2G00104250 Rroxscaffold_2G00149290
rosa_rugosa	Rorug02G0024700 Rorug02G0366400
rosa_samantha	Rh2AG070400 Rh2AG417800 Rh2BG069900 Rh2BG427700 Rh2CG071100 Rh2CG356200 Rh2CG404100 Rh2DG068900 Rh2DG437200
rosa_wichuraiana	Rw2G006190 Rw2G034200 Rw2G034260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1312, 1482
AccB1I	GGYRCC	1 cut(s)	338
AccB7I	CCANNNNNTGG	1 cut(s)	505
AccI	GTMKAC	1 cut(s)	1256
AccII	CGCG	2 cut(s)	1059, 1409
AciI	CCGC	3 cut(s)	611, 946, 1057
AclWI	GGATC	1 cut(s)	1075
AcsI	RAATTY	1 cut(s)	1157
AcuI	CTGAAG	1 cut(s)	240
AfaI	GTAC	6 cut(s)	5, 192, 376, 1233, 1461, 1467
AfiI	CCNNNNNNNGG	7 cut(s)	24, 98, 505, 625, 942, 1030, 1031
AflIII	ACRYGT	2 cut(s)	910, 1462
AgsI	TTSAA	8 cut(s)	206, 267, 706, 1066, 1102, 1114, 1366, 1390
AjnI	CCWGG	3 cut(s)	166, 1024, 1213
AluBI	AGCT	8 cut(s)	80, 114, 132, 254, 568, 893, 917, 1237
AluI	AGCT	8 cut(s)	80, 114, 132, 254, 568, 893, 917, 1237
Alw21I	GWGCWC	3 cut(s)	134, 570, 1359
Alw26I	GTCTC	2 cut(s)	110, 314
AlwI	GGATC	1 cut(s)	1075
AlwNI	CAGNNNCTG	3 cut(s)	194, 773, 988
AoxI	GGCC	4 cut(s)	37, 54, 616, 1021
ApaI	GGGCCC	1 cut(s)	1025
ApeKI	GCWGC	8 cut(s)	11, 77, 278, 711, 767, 1184, 1265, 1488
ApoI	RAATTY	1 cut(s)	1157
Asp700I	GAANNNNTTC	1 cut(s)	813
AspLEI	GCGC	1 cut(s)	1411
AspS9I	GGNCC	6 cut(s)	27, 37, 54, 616, 1021, 1022
AsuC2I	CCSGG	3 cut(s)	61, 936, 1470
AsuHPI	GGTGA	1 cut(s)	1419
AsuNHI	GCTAGC	1 cut(s)	1142
AvaII	GGWCC	1 cut(s)	27
BaeGI	GKGCMC	2 cut(s)	142, 1025
BaeI	ACNNNNGTAYC	2 cut(s)	312, 345
BanI	GGYRCC	1 cut(s)	338
BanII	GRGCYC	3 cut(s)	134, 570, 1025
BbsI	GAAGAC	1 cut(s)	1373
Bbv12I	GWGCWC	3 cut(s)	134, 570, 1359
BbvI	GCAGC	8 cut(s)	23, 89, 290, 723, 779, 1196, 1252, 1500
BccI	CCATC	2 cut(s)	496, 985
BceAI	ACGGC	2 cut(s)	24, 947
BcgI	CGANNNNNNTGC	2 cut(s)	66, 100
BciT130I	CCWGG	3 cut(s)	168, 1026, 1215
BciVI	GTATCC	3 cut(s)	340, 808, 1083
BclI	TGATCA	1 cut(s)	781
BcnI	CCSGG	3 cut(s)	61, 936, 1470
BcoDI	GTCTC	2 cut(s)	110, 314
BfaI	CTAG	5 cut(s)	353, 450, 1143, 1283, 1290
BfmI	CTRYAG	2 cut(s)	276, 1266
BfuAI	ACCTGC	2 cut(s)	1312, 1482
BfuI	GTATCC	3 cut(s)	340, 808, 1083
BisI	GCNGC	9 cut(s)	12, 78, 279, 712, 768, 947, 1185, 1266, 1489
BlsI	GCNGC	9 cut(s)	13, 79, 280, 713, 769, 948, 1186, 1267, 1490
Bme1390I	CCNGG	6 cut(s)	61, 168, 936, 1026, 1215, 1470
Bme18I	GGWCC	1 cut(s)	27
BmgT120I	GGNCC	6 cut(s)	27, 37, 54, 616, 1021, 1022
BmrFI	CCNGG	6 cut(s)	61, 168, 936, 1026, 1215, 1470
BmsI	GCATC	6 cut(s)	466, 723, 779, 1026, 1172, 1310
BmtI	GCTAGC	1 cut(s)	1146
BoxI	GACNNNNGTC	1 cut(s)	25
BpiI	GAAGAC	1 cut(s)	1373
BplI	GAGNNNNNCTC	2 cut(s)	823, 855
BpmI	CTGGAG	2 cut(s)	171, 965
BpuEI	CTTGAG	3 cut(s)	17, 118, 1027
BpuMI	CCSGG	3 cut(s)	61, 936, 1470
BsaAI	YACGTR	1 cut(s)	913
BsaI	GGTCTC	1 cut(s)	110
BsaJI	CCNNGG	4 cut(s)	40, 166, 1024, 1248
BsaWI	WCCGGW	2 cut(s)	384, 901
Bsc4I	CCNNNNNNNGG	7 cut(s)	24, 98, 505, 625, 942, 1030, 1031
Bse1I	ACTGG	7 cut(s)	188, 326, 333, 441, 982, 1153, 1443
Bse3DI	GCAATG	3 cut(s)	729, 801, 1459
BseBI	CCWGG	3 cut(s)	168, 1026, 1215
BseDI	CCNNGG	4 cut(s)	40, 166, 1024, 1248
BseGI	GGATG	2 cut(s)	1041, 1234
BseLI	CCNNNNNNNGG	7 cut(s)	24, 98, 505, 625, 942, 1030, 1031
BseMI	GCAATG	3 cut(s)	729, 801, 1459
BseMII	CTCAG	2 cut(s)	37, 1296
BseNI	ACTGG	7 cut(s)	188, 326, 333, 441, 982, 1153, 1443
BseRI	GAGGAG	4 cut(s)	174, 741, 863, 888
BseSI	GKGCMC	2 cut(s)	142, 1025
BseXI	GCAGC	8 cut(s)	23, 89, 290, 723, 779, 1196, 1252, 1500
BseYI	CCCAGC	1 cut(s)	1016
BsgI	GTGCAG	2 cut(s)	96, 599
Bsh1236I	CGCG	2 cut(s)	1059, 1409
BshFI	GGCC	4 cut(s)	39, 56, 618, 1023
BshNI	GGYRCC	1 cut(s)	338
BsiHKAI	GWGCWC	3 cut(s)	134, 570, 1359
BsiSI	CCGG	5 cut(s)	61, 385, 902, 936, 1469
BslFI	GGGAC	1 cut(s)	13
BslI	CCNNNNNNNGG	7 cut(s)	24, 98, 505, 625, 942, 1030, 1031
BsmAI	GTCTC	2 cut(s)	110, 314
BsmFI	GGGAC	1 cut(s)	13
BsmI	GAATGC	1 cut(s)	1097
BsnI	GGCC	4 cut(s)	39, 56, 618, 1023
Bso31I	GGTCTC	1 cut(s)	110
Bsp120I	GGGCCC	1 cut(s)	1021
Bsp1286I	GDGCHC	5 cut(s)	134, 142, 570, 1025, 1359
Bsp1407I	TGTACA	3 cut(s)	3, 1231, 1459
Bsp143I	GATC	2 cut(s)	781, 1080
BspACI	CCGC	3 cut(s)	611, 946, 1057
BspANI	GGCC	4 cut(s)	39, 56, 618, 1023
BspCNI	CTCAG	2 cut(s)	36, 1297
BspFNI	CGCG	2 cut(s)	1059, 1409
BspMAI	CTGCAG	2 cut(s)	280, 1270
BspMI	ACCTGC	2 cut(s)	1312, 1482
BspOI	GCTAGC	1 cut(s)	1146
BspPI	GGATC	1 cut(s)	1075
BspQI	GCTCTTC	1 cut(s)	558
BspT107I	GGYRCC	1 cut(s)	338
BspTNI	GGTCTC	1 cut(s)	110
BsrDI	GCAATG	3 cut(s)	729, 801, 1459
BsrGI	TGTACA	3 cut(s)	3, 1231, 1459
BsrI	ACTGG	7 cut(s)	188, 326, 333, 441, 982, 1153, 1443
BssECI	CCNNGG	4 cut(s)	40, 166, 1024, 1248
BssMI	GATC	2 cut(s)	781, 1080
BssT1I	CCWWGG	1 cut(s)	1248
Bst2UI	CCWGG	3 cut(s)	168, 1026, 1215
Bst4CI	ACNGT	2 cut(s)	976, 1372
Bst6I	CTCTTC	2 cut(s)	558, 1429
BstAPI	GCANNNNNTGC	1 cut(s)	74
BstAUI	TGTACA	3 cut(s)	3, 1231, 1459
BstBAI	YACGTR	1 cut(s)	913
BstC8I	GCNNGC	5 cut(s)	116, 582, 944, 960, 1144
BstDEI	CTNAG	3 cut(s)	23, 603, 1305
BstDSI	CCRYGG	1 cut(s)	40
BstF5I	GGATG	2 cut(s)	1041, 1234
BstFNI	CGCG	2 cut(s)	1059, 1409
BstHHI	GCGC	1 cut(s)	1411
BstKTI	GATC	2 cut(s)	784, 1083
BstMAI	GTCTC	2 cut(s)	110, 314
BstMBI	GATC	2 cut(s)	781, 1080
BstMWI	GCNNNNNNNGC	5 cut(s)	74, 396, 955, 1045, 1169
BstNI	CCWGG	3 cut(s)	168, 1026, 1215
BstNSI	RCATGY	1 cut(s)	1466
BstPAI	GACNNNNGTC	1 cut(s)	25
BstSCI	CCNGG	6 cut(s)	59, 166, 934, 1024, 1213, 1468
BstSFI	CTRYAG	2 cut(s)	276, 1266
BstSLI	GKGCMC	2 cut(s)	142, 1025
BstUI	CGCG	2 cut(s)	1059, 1409
BstV1I	GCAGC	8 cut(s)	23, 89, 290, 723, 779, 1196, 1252, 1500
BstV2I	GAAGAC	1 cut(s)	1373
BstX2I	RGATCY	1 cut(s)	1080
BstYI	RGATCY	1 cut(s)	1080
BsuI	GTATCC	3 cut(s)	340, 808, 1083
BsuRI	GGCC	4 cut(s)	39, 56, 618, 1023
BtgI	CCRYGG	1 cut(s)	40
BtsCI	GGATG	2 cut(s)	1041, 1234
BtsIMutI	CAGTG	5 cut(s)	340, 448, 1146, 1377, 1436
BveI	ACCTGC	2 cut(s)	1312, 1482
Cac8I	GCNNGC	5 cut(s)	116, 582, 944, 960, 1144
CaiI	CAGNNNCTG	3 cut(s)	194, 773, 988
CfoI	GCGC	1 cut(s)	1411
Cfr13I	GGNCC	6 cut(s)	27, 37, 54, 616, 1021, 1022
CseI	GACGC	2 cut(s)	270, 1048
Csp6I	GTAC	6 cut(s)	4, 191, 375, 1232, 1460, 1466
CspCI	CAANNNNNGTGG	4 cut(s)	22, 46, 57, 81
CviAII	CATG	2 cut(s)	1463, 1499
CviQI	GTAC	6 cut(s)	4, 191, 375, 1232, 1460, 1466
DdeI	CTNAG	3 cut(s)	23, 603, 1305
DpnI	GATC	2 cut(s)	783, 1082
DpnII	GATC	2 cut(s)	781, 1080
Eam1104I	CTCTTC	2 cut(s)	558, 1429
EarI	CTCTTC	2 cut(s)	558, 1429
Ecl136II	GAGCTC	2 cut(s)	132, 568
Eco130I	CCWWGG	1 cut(s)	1248
Eco24I	GRGCYC	3 cut(s)	134, 570, 1025
Eco31I	GGTCTC	1 cut(s)	110
Eco47I	GGWCC	1 cut(s)	27
Eco53kI	GAGCTC	2 cut(s)	132, 568
Eco57I	CTGAAG	1 cut(s)	240
EcoICRI	GAGCTC	2 cut(s)	132, 568
EcoO109I	RGGNCCY	4 cut(s)	27, 616, 1021, 1022
EcoRII	CCWGG	3 cut(s)	166, 1024, 1213
EcoT14I	CCWWGG	1 cut(s)	1248
EcoT38I	GRGCYC	3 cut(s)	134, 570, 1025
ErhI	CCWWGG	1 cut(s)	1248
FaeI	CATG	2 cut(s)	1466, 1502
FalI	AAGNNNNNCTT	2 cut(s)	951, 983
FaqI	GGGAC	1 cut(s)	13
FatI	CATG	2 cut(s)	1462, 1498
FauNDI	CATATG	1 cut(s)	1447
FbaI	TGATCA	1 cut(s)	781
FblI	GTMKAC	1 cut(s)	1256
Fnu4HI	GCNGC	9 cut(s)	12, 78, 279, 712, 768, 947, 1185, 1266, 1489
FokI	GGATG	2 cut(s)	1048, 1241
FriOI	GRGCYC	3 cut(s)	134, 570, 1025
Fsp4HI	GCNGC	9 cut(s)	12, 78, 279, 712, 768, 947, 1185, 1266, 1489
FspBI	CTAG	5 cut(s)	353, 450, 1143, 1283, 1290
GlaI	GCGC	1 cut(s)	1410
GluI	GCNGC	9 cut(s)	12, 78, 279, 712, 768, 947, 1185, 1266, 1489
GsaI	CCCAGC	1 cut(s)	1020
GsuI	CTGGAG	2 cut(s)	171, 965
HaeIII	GGCC	4 cut(s)	39, 56, 618, 1023
HapII	CCGG	5 cut(s)	61, 385, 902, 936, 1469
HgaI	GACGC	2 cut(s)	270, 1048
HhaI	GCGC	1 cut(s)	1411
Hin1II	CATG	2 cut(s)	1466, 1502
Hin6I	GCGC	1 cut(s)	1409
HinP1I	GCGC	1 cut(s)	1409
HincII	GTYRAC	3 cut(s)	175, 520, 1257
HindII	GTYRAC	3 cut(s)	175, 520, 1257
HpaI	GTTAAC	1 cut(s)	175
HpaII	CCGG	5 cut(s)	61, 385, 902, 936, 1469
HphI	GGTGA	1 cut(s)	1419
Hpy166II	GTNNAC	3 cut(s)	175, 520, 1257
Hpy188I	TCNGA	5 cut(s)	259, 685, 823, 1156, 1306
Hpy188III	TCNNGA	7 cut(s)	18, 484, 788, 1063, 1283, 1290, 1363
Hpy8I	GTNNAC	3 cut(s)	175, 520, 1257
HpyAV	CCTTC	6 cut(s)	102, 152, 188, 550, 1311, 1370
HpyCH4III	ACNGT	2 cut(s)	976, 1372
HpyCH4IV	ACGT	3 cut(s)	912, 1243, 1385
HpyF10VI	GCNNNNNNNGC	5 cut(s)	74, 396, 955, 1045, 1169
HpyF3I	CTNAG	3 cut(s)	23, 603, 1305
HpySE526I	ACGT	3 cut(s)	912, 1243, 1385
Hsp92II	CATG	2 cut(s)	1466, 1502
HspAI	GCGC	1 cut(s)	1409
Ksp22I	TGATCA	1 cut(s)	781
KspAI	GTTAAC	1 cut(s)	175
Kzo9I	GATC	2 cut(s)	781, 1080
LguI	GCTCTTC	1 cut(s)	558
LmnI	GCTCC	7 cut(s)	387, 437, 454, 721, 754, 850, 1354
Lsp1109I	GCAGC	8 cut(s)	23, 89, 290, 723, 779, 1196, 1252, 1500
LweI	GCATC	6 cut(s)	466, 723, 779, 1026, 1172, 1310
MaeI	CTAG	5 cut(s)	353, 450, 1143, 1283, 1290
MaeII	ACGT	3 cut(s)	912, 1243, 1385
MaeIII	GTNAC	4 cut(s)	428, 1222, 1381, 1472
MalI	GATC	2 cut(s)	783, 1082
MboI	GATC	2 cut(s)	781, 1080
MboII	GAAGA	4 cut(s)	575, 1378, 1416, 1426
MfeI	CAATTG	1 cut(s)	1203
MflI	RGATCY	1 cut(s)	1080
MhlI	GDGCHC	5 cut(s)	134, 142, 570, 1025, 1359
MluCI	AATT	5 cut(s)	739, 1157, 1193, 1203, 1482
MlyI	GAGTC	5 cut(s)	15, 280, 515, 1262, 1472
MmeI	TCCRAC	1 cut(s)	850
MroXI	GAANNNNTTC	1 cut(s)	813
MseI	TTAA	3 cut(s)	174, 369, 1274
MspA1I	CMGCKG	1 cut(s)	80
MspI	CCGG	5 cut(s)	61, 385, 902, 936, 1469
MspR9I	CCNGG	6 cut(s)	61, 168, 936, 1026, 1215, 1470
MunI	CAATTG	1 cut(s)	1203
Mva1269I	GAATGC	1 cut(s)	1097
MvaI	CCWGG	3 cut(s)	168, 1026, 1215
MvnI	CGCG	2 cut(s)	1059, 1409
MwoI	GCNNNNNNNGC	5 cut(s)	74, 396, 955, 1045, 1169
NciI	CCSGG	3 cut(s)	61, 936, 1470
NdeI	CATATG	1 cut(s)	1447
NdeII	GATC	2 cut(s)	781, 1080
NheI	GCTAGC	1 cut(s)	1142
NlaIII	CATG	2 cut(s)	1466, 1502
NmuCI	GTSAC	2 cut(s)	428, 1381
NspI	RCATGY	1 cut(s)	1466
PciI	ACATGT	1 cut(s)	1462
PciSI	GCTCTTC	1 cut(s)	558
PctI	GAATGC	1 cut(s)	1097
PdmI	GAANNNNTTC	1 cut(s)	813
PfeI	GAWTC	8 cut(s)	302, 607, 634, 686, 1066, 1118, 1279, 1286
PflMI	CCANNNNNTGG	1 cut(s)	505
PkrI	GCNGC	9 cut(s)	13, 79, 280, 713, 769, 948, 1186, 1267, 1490
PleI	GAGTC	5 cut(s)	15, 279, 515, 1261, 1472
PpsI	GAGTC	5 cut(s)	15, 279, 515, 1261, 1472
Ppu21I	YACGTR	1 cut(s)	913
PpuMI	RGGWCCY	1 cut(s)	27
PscI	ACATGT	1 cut(s)	1462
PshAI	GACNNNNGTC	1 cut(s)	25
Psp124BI	GAGCTC	2 cut(s)	134, 570
Psp5II	RGGWCCY	1 cut(s)	27
Psp6I	CCWGG	3 cut(s)	166, 1024, 1213
PspFI	CCCAGC	1 cut(s)	1016
PspGI	CCWGG	3 cut(s)	166, 1024, 1213
PspOMI	GGGCCC	1 cut(s)	1021
PspPI	GGNCC	6 cut(s)	27, 37, 54, 616, 1021, 1022
PspPPI	RGGWCCY	1 cut(s)	27
PstI	CTGCAG	2 cut(s)	280, 1270
PstNI	CAGNNNCTG	3 cut(s)	194, 773, 988
PsuI	RGATCY	1 cut(s)	1080
PvuII	CAGCTG	1 cut(s)	80
RsaI	GTAC	6 cut(s)	5, 192, 376, 1233, 1461, 1467
RsaNI	GTAC	6 cut(s)	4, 191, 375, 1232, 1460, 1466
SacI	GAGCTC	2 cut(s)	134, 570
SalI	GTCGAC	1 cut(s)	1255
SapI	GCTCTTC	1 cut(s)	558
SaqAI	TTAA	3 cut(s)	174, 369, 1274
SatI	GCNGC	9 cut(s)	12, 78, 279, 712, 768, 947, 1185, 1266, 1489
Sau3AI	GATC	2 cut(s)	781, 1080
Sau96I	GGNCC	6 cut(s)	27, 37, 54, 616, 1021, 1022
SchI	GAGTC	5 cut(s)	15, 280, 515, 1262, 1472
ScrFI	CCNGG	6 cut(s)	61, 168, 936, 1026, 1215, 1470
SduI	GDGCHC	5 cut(s)	134, 142, 570, 1025, 1359
SfaNI	GCATC	6 cut(s)	466, 723, 779, 1026, 1172, 1310
SfcI	CTRYAG	2 cut(s)	276, 1266
SinI	GGWCC	1 cut(s)	27
SmlI	CTYRAG	3 cut(s)	32, 133, 1042
SmoI	CTYRAG	3 cut(s)	32, 133, 1042
Sse9I	AATT	5 cut(s)	739, 1157, 1193, 1203, 1482
SsiI	CCGC	3 cut(s)	611, 946, 1057
SspMI	CTAG	5 cut(s)	353, 450, 1143, 1283, 1290
SstI	GAGCTC	2 cut(s)	134, 570
StyD4I	CCNGG	6 cut(s)	59, 166, 934, 1024, 1213, 1468
StyI	CCWWGG	1 cut(s)	1248
TaaI	ACNGT	2 cut(s)	976, 1372
TaiI	ACGT	3 cut(s)	915, 1246, 1388
TaqI	TCGA	4 cut(s)	86, 846, 1166, 1256
TasI	AATT	5 cut(s)	739, 1157, 1193, 1203, 1482
TatI	WGTACW	4 cut(s)	3, 374, 1231, 1459
TauI	GCSGC	1 cut(s)	949
TfiI	GAWTC	8 cut(s)	302, 607, 634, 686, 1066, 1118, 1279, 1286
Tru1I	TTAA	3 cut(s)	174, 369, 1274
Tru9I	TTAA	3 cut(s)	174, 369, 1274
TscAI	CASTG	5 cut(s)	340, 448, 1153, 1377, 1443
TseFI	GTSAC	2 cut(s)	428, 1381
TseI	GCWGC	8 cut(s)	11, 77, 278, 711, 767, 1184, 1265, 1488
Tsp45I	GTSAC	2 cut(s)	428, 1381
TspDTI	ATGAA	5 cut(s)	84, 576, 626, 718, 1292
TspGWI	ACGGA	3 cut(s)	872, 1241, 1350
TspRI	CASTG	5 cut(s)	340, 448, 1153, 1377, 1443
Van91I	CCANNNNNTGG	1 cut(s)	505
VpaK11BI	GGWCC	1 cut(s)	27
XapI	RAATTY	1 cut(s)	1157
XbaI	TCTAGA	2 cut(s)	1282, 1289
XceI	RCATGY	1 cut(s)	1466
XmiI	GTMKAC	1 cut(s)	1256
XmnI	GAANNNNTTC	1 cut(s)	813
XspI	CTAG	5 cut(s)	353, 450, 1143, 1283, 1290

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.