Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0002641

Physical Location & Seq

Genomic Coordinates Forward (+)

18343599 .. 18348131

Gene Structure 4533 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1200100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGTACAAGGCAGCCTCCCGACTCAGGTCCCTCAAGGGCCGTGGAAATTTGGGGGCCACCCGGTTTGCAACAAGTGCAGCTGTATCGAAGCCATCCTCAGGCGGTCTCTTTAGTTGGCTGACCGGGGAGAGCTCAAGTGCTCTTCCTCCGTTGGAAACCCAGCTCCCAGGTGTTAACCTCCCTCCTCCAGTACCTGATTATGTTGAACCAAGCAAAACTCAGATTACAACTCTTTCTAATGGTGTCAAAATAGCTTCTGAAACTTCAACGAGTCCTGCAGCGTCCATTGGAATATATGTGGATTCTGGTTCCATATACGAGACACCAGTATCCAGTGGTGCCTCACACTTGCTAGAACGATTGGCTTTTAAGAGTACAACCAACCGGAGCCACTTACGCATTGTGAGAGAAGTGGAAGCAATCGGTGGTCACATAGGAGCCAGTGCCTCTAGGGAGCAAATGGGATATACTTTTGATGCCATCAAGACCTATGTTCCCCAGATGGTGGAACTGCTTGTTGACTCTGTGAGGAATCCTGCCTTCTTGGAATGGGAAGTCAATGAAGAGCTCAAAAAAGTGCGGGCAGAGATTGGAGAACTCTCAAAGAATCCCCAGGGCTTACTGTTGGAGGCAATTCATTCTGCTGGTTATTCTGGTGCATTGGCTAATCCTCTTTTGGCTTCTGAAGCATCACTGAACAGATTGAATGGCAGCATCTTGGAGCAGTTCATTGCTGAAAATTATACTGCTCCTCGTATAGTTCTTGCAGCATCTGGGGTTGATCATCAGGAGATTGTATCCATTGCTGAACCTCTTCTCTCTGACCTACCGAGTGTGCCTCGCTATGAGGAGCCACTATCCAAATATGTTGGAGGAGAGTACCGTTATCAAGCTGATACACCGGAAACACACGTAGCTCTTGCTTTTGAGGCTCCCGGTGGCTGGCGCGAGGAGAAGCAAGCCATTCTTTTGACAGTTCTCCAGTTGCTTATGGGAGGTGGTGGATCTTTCTCCGCTGGGGGCCCCGGAAAGGGGATGCACTCAAGGCTATATCTCCGCGTCTTGAATCAGTATCCAGAGATCCAATCCTTTTCTGCATTCAACAGCATCTTTAATGATTCTGGGTTGTTTGGTATTTATGCTAGCACTGGCTCTGAATTTGCATCAAAAGCAGTTGATATAGCAGCACAAGAATTACTTACAATTGCAACACCTGGACAAGTTACGGATGTACAGCTTAAACGTGCCAAGGAGTCCACAAAGGCGGCAGTTTTAATGAATCTAGAATCTAGAATGATTGCATCAGAGGATATAGGAAGGCAGGTTTTGACATACGGAGAGAGGAAACCTGTGGAGCACTTCTTGAAGACGGTAGAAGAAGTGAGCTTGAAAGATATTACTACAATCGCGCAGAAGATTATTTCTTCACCGCTAACAATGGCAACATATGGCAATGTTGTACATGTACCCGGTTACGACTCAATTAGCAGCAGGTTCCAAGCAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006508 GO:0006605 GO:0006626 GO:0006627 GO:0006807 GO:0006810 GO:0006839 GO:0006886 GO:0006950 GO:0006970 GO:0006996 GO:0007005 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010467 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016485 GO:0016787 GO:0017076 GO:0019538 GO:0019866 GO:0019867 GO:0030554 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0034982 GO:0035639 GO:0036094 GO:0042221 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045184 GO:0045275 GO:0046872 GO:0046907 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0051604 GO:0051641 GO:0051649 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0070585 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072655 GO:0097159 GO:0097367 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M16 Peptidase_M16_C

Protein Analysis

503

Amino Acids

53.97

Weight (kDa)

5.83

Isoelectric Point (pI)

37.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	84 - 227	1.5e-40	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	240 - 418	1.4e-30	Peptidase M16 inactive domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002641)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51980 AT1G51980 AT3G16480
fragaria_vesca	FvH4_1g06540 FvH4_1g06540 FvH4_1g06540 FvH4_6g32600 FvH4_6g32600
malus_domestica	MD02G1068800.v1.1 MD09G1200100.v1.1 MD17G1181200.v1.1
prunus_persica	Prupe.3G056700_v2.0.a1 Prupe.7G216900_v2.0.a1
pyrus_communis	pycom02g05450 pycom09g11770 pycom17g19060
rosa_chinensis	RchiOBHm_Chr2g0092291 RchiOBHm_Chr2g0141051
rosa_laevigata	RLG00000016294 RLG00000019911
rosa_multiflora	Rmu_co8416335.1_g000001 Rmu_sc0003289.1_g000021 Rmu_sc0004769.1_g000017 Rmu_sc0012897.1_g000005
rosa_roxburghii	Rroxscaffold_2G00104250 Rroxscaffold_2G00149290
rosa_rugosa	Rorug02G0024700 Rorug02G0366400
rosa_samantha	Rh2AG070400 Rh2AG417800 Rh2BG069900 Rh2BG427700 Rh2CG071100 Rh2CG356200 Rh2CG404100 Rh2DG068900 Rh2DG437200
rosa_wichuraiana	Rw2G006190 Rw2G034200 Rw2G034260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1312, 1482
AccB1I	GGYRCC	1 cut(s)	338
AccB7I	CCANNNNNTGG	1 cut(s)	505
AccII	CGCG	3 cut(s)	948, 1059, 1409
AciI	CCGC	6 cut(s)	102, 580, 1014, 1057, 1265, 1430
AclWI	GGATC	2 cut(s)	1012, 1075
AcsI	RAATTY	2 cut(s)	46, 1157
AcuI	CTGAAG	1 cut(s)	705
AfaI	GTAC	7 cut(s)	5, 192, 376, 881, 1233, 1461, 1467
AfiI	CCNNNNNNNGG	7 cut(s)	24, 98, 505, 942, 1030, 1031, 1032
AflIII	ACRYGT	2 cut(s)	910, 1462
AgsI	TTSAA	7 cut(s)	206, 267, 706, 1066, 1102, 1366, 1390
AjnI	CCWGG	3 cut(s)	166, 612, 1213
AluBI	AGCT	9 cut(s)	80, 132, 163, 254, 568, 893, 917, 1237, 1386
AluI	AGCT	9 cut(s)	80, 132, 163, 254, 568, 893, 917, 1237, 1386
Alw21I	GWGCWC	4 cut(s)	134, 142, 570, 1359
Alw26I	GTCTC	2 cut(s)	110, 314
AlwI	GGATC	2 cut(s)	1012, 1075
AlwNI	CAGNNNCTG	2 cut(s)	194, 773
AoxI	GGCC	3 cut(s)	37, 54, 1021
ApaI	GGGCCC	1 cut(s)	1025
ApeKI	GCWGC	7 cut(s)	11, 77, 278, 711, 767, 1184, 1488
ApoI	RAATTY	2 cut(s)	46, 1157
Asp700I	GAANNNNTTC	1 cut(s)	813
AspLEI	GCGC	2 cut(s)	948, 1411
AspS9I	GGNCC	5 cut(s)	27, 37, 54, 1021, 1022
AsuC2I	CCSGG	5 cut(s)	61, 124, 936, 1026, 1470
AsuHPI	GGTGA	1 cut(s)	1419
AsuNHI	GCTAGC	1 cut(s)	1142
AvaII	GGWCC	1 cut(s)	27
AxyI	CCTNAGG	1 cut(s)	97
BaeGI	GKGCMC	1 cut(s)	1025
BaeI	ACNNNNGTAYC	2 cut(s)	312, 345
BanI	GGYRCC	1 cut(s)	338
BanII	GRGCYC	3 cut(s)	134, 570, 1025
BbsI	GAAGAC	1 cut(s)	1373
Bbv12I	GWGCWC	4 cut(s)	134, 142, 570, 1359
BbvI	GCAGC	7 cut(s)	23, 89, 290, 723, 779, 1196, 1500
BccI	CCATC	3 cut(s)	100, 488, 496
BceAI	ACGGC	1 cut(s)	24
BcgI	CGANNNNNNTGC	2 cut(s)	66, 100
BciT130I	CCWGG	3 cut(s)	168, 614, 1215
BciVI	GTATCC	3 cut(s)	340, 808, 1083
BclI	TGATCA	1 cut(s)	781
BcnI	CCSGG	5 cut(s)	61, 124, 936, 1026, 1470
BcoDI	GTCTC	2 cut(s)	110, 314
BfaI	CTAG	5 cut(s)	353, 450, 1143, 1283, 1290
BfmI	CTRYAG	1 cut(s)	276
BfuAI	ACCTGC	2 cut(s)	1312, 1482
BfuI	GTATCC	3 cut(s)	340, 808, 1083
BisI	GCNGC	8 cut(s)	12, 78, 279, 712, 768, 1185, 1266, 1489
BlsI	GCNGC	8 cut(s)	13, 79, 280, 713, 769, 1186, 1267, 1490
Bme1390I	CCNGG	8 cut(s)	61, 124, 168, 614, 936, 1026, 1215, 1470
Bme18I	GGWCC	1 cut(s)	27
BmgT120I	GGNCC	5 cut(s)	27, 37, 54, 1021, 1022
BmrFI	CCNGG	8 cut(s)	61, 124, 168, 614, 936, 1026, 1215, 1470
BmsI	GCATC	8 cut(s)	466, 698, 723, 779, 1026, 1116, 1172, 1310
BmtI	GCTAGC	1 cut(s)	1146
BoxI	GACNNNNGTC	1 cut(s)	25
BpiI	GAAGAC	1 cut(s)	1373
BplI	GAGNNNNNCTC	2 cut(s)	823, 855
BpmI	CTGGAG	2 cut(s)	171, 965
BpuEI	CTTGAG	3 cut(s)	17, 118, 1027
BpuMI	CCSGG	5 cut(s)	61, 124, 936, 1026, 1470
BsaAI	YACGTR	1 cut(s)	913
BsaI	GGTCTC	1 cut(s)	110
BsaJI	CCNNGG	7 cut(s)	40, 123, 166, 612, 613, 1024, 1248
BsaWI	WCCGGW	2 cut(s)	384, 901
Bsc4I	CCNNNNNNNGG	7 cut(s)	24, 98, 505, 942, 1030, 1031, 1032
Bse1I	ACTGG	6 cut(s)	188, 326, 333, 441, 982, 1153
Bse21I	CCTNAGG	1 cut(s)	97
Bse3DI	GCAATG	3 cut(s)	729, 801, 1459
BseBI	CCWGG	3 cut(s)	168, 614, 1215
BseDI	CCNNGG	7 cut(s)	40, 123, 166, 612, 613, 1024, 1248
BseGI	GGATG	3 cut(s)	92, 1041, 1234
BseLI	CCNNNNNNNGG	7 cut(s)	24, 98, 505, 942, 1030, 1031, 1032
BseMI	GCAATG	3 cut(s)	729, 801, 1459
BseMII	CTCAG	3 cut(s)	37, 111, 233
BseNI	ACTGG	6 cut(s)	188, 326, 333, 441, 982, 1153
BseRI	GAGGAG	5 cut(s)	174, 741, 863, 888, 965
BseSI	GKGCMC	1 cut(s)	1025
BseXI	GCAGC	7 cut(s)	23, 89, 290, 723, 779, 1196, 1500
BseYI	CCCAGC	2 cut(s)	159, 1016
BsgI	GTGCAG	1 cut(s)	96
Bsh1236I	CGCG	3 cut(s)	948, 1059, 1409
BshFI	GGCC	3 cut(s)	39, 56, 1023
BshNI	GGYRCC	1 cut(s)	338
BsiHKAI	GWGCWC	4 cut(s)	134, 142, 570, 1359
BsiSI	CCGG	7 cut(s)	61, 123, 385, 902, 936, 1026, 1470
BslFI	GGGAC	1 cut(s)	13
BslI	CCNNNNNNNGG	7 cut(s)	24, 98, 505, 942, 1030, 1031, 1032
BsmAI	GTCTC	2 cut(s)	110, 314
BsmFI	GGGAC	1 cut(s)	13
BsmI	GAATGC	1 cut(s)	1097
BsnI	GGCC	3 cut(s)	39, 56, 1023
Bso31I	GGTCTC	1 cut(s)	110
Bsp120I	GGGCCC	1 cut(s)	1021
Bsp1286I	GDGCHC	5 cut(s)	134, 142, 570, 1025, 1359
Bsp1407I	TGTACA	3 cut(s)	3, 1231, 1459
Bsp143I	GATC	3 cut(s)	781, 1004, 1080
BspACI	CCGC	6 cut(s)	102, 580, 1014, 1057, 1265, 1430
BspANI	GGCC	3 cut(s)	39, 56, 1023
BspCNI	CTCAG	3 cut(s)	36, 110, 232
BspFNI	CGCG	3 cut(s)	948, 1059, 1409
BspMAI	CTGCAG	1 cut(s)	280
BspMI	ACCTGC	2 cut(s)	1312, 1482
BspOI	GCTAGC	1 cut(s)	1146
BspPI	GGATC	2 cut(s)	1012, 1075
BspQI	GCTCTTC	2 cut(s)	147, 558
BspT107I	GGYRCC	1 cut(s)	338
BspTNI	GGTCTC	1 cut(s)	110
BsrDI	GCAATG	3 cut(s)	729, 801, 1459
BsrGI	TGTACA	3 cut(s)	3, 1231, 1459
BsrI	ACTGG	6 cut(s)	188, 326, 333, 441, 982, 1153
BssECI	CCNNGG	7 cut(s)	40, 123, 166, 612, 613, 1024, 1248
BssMI	GATC	3 cut(s)	781, 1004, 1080
BssT1I	CCWWGG	1 cut(s)	1248
Bst2UI	CCWGG	3 cut(s)	168, 614, 1215
Bst4CI	ACNGT	4 cut(s)	624, 884, 976, 1372
Bst6I	CTCTTC	3 cut(s)	147, 558, 819
BstAPI	GCANNNNNTGC	1 cut(s)	74
BstAUI	TGTACA	3 cut(s)	3, 1231, 1459
BstBAI	YACGTR	1 cut(s)	913
BstC8I	GCNNGC	4 cut(s)	582, 944, 960, 1144
BstDEI	CTNAG	3 cut(s)	23, 97, 219
BstDSI	CCRYGG	1 cut(s)	40
BstF5I	GGATG	3 cut(s)	92, 1041, 1234
BstFNI	CGCG	3 cut(s)	948, 1059, 1409
BstHHI	GCGC	2 cut(s)	948, 1411
BstKTI	GATC	3 cut(s)	784, 1007, 1083
BstMAI	GTCTC	2 cut(s)	110, 314
BstMBI	GATC	3 cut(s)	781, 1004, 1080
BstMWI	GCNNNNNNNGC	6 cut(s)	74, 396, 686, 929, 1045, 1169
BstNI	CCWGG	3 cut(s)	168, 614, 1215
BstNSI	RCATGY	1 cut(s)	1466
BstPAI	GACNNNNGTC	1 cut(s)	25
BstSCI	CCNGG	8 cut(s)	59, 122, 166, 612, 934, 1024, 1213, 1468
BstSFI	CTRYAG	1 cut(s)	276
BstSLI	GKGCMC	1 cut(s)	1025
BstUI	CGCG	3 cut(s)	948, 1059, 1409
BstV1I	GCAGC	7 cut(s)	23, 89, 290, 723, 779, 1196, 1500
BstV2I	GAAGAC	1 cut(s)	1373
BstX2I	RGATCY	2 cut(s)	1004, 1080
BstYI	RGATCY	2 cut(s)	1004, 1080
Bsu36I	CCTNAGG	1 cut(s)	97
BsuI	GTATCC	3 cut(s)	340, 808, 1083
BsuRI	GGCC	3 cut(s)	39, 56, 1023
BtgI	CCRYGG	1 cut(s)	40
BtsCI	GGATG	3 cut(s)	92, 1041, 1234
BtsIMutI	CAGTG	4 cut(s)	340, 448, 692, 1146
BveI	ACCTGC	2 cut(s)	1312, 1482
Cac8I	GCNNGC	4 cut(s)	582, 944, 960, 1144
CaiI	CAGNNNCTG	2 cut(s)	194, 773
CfoI	GCGC	2 cut(s)	948, 1411
Cfr13I	GGNCC	5 cut(s)	27, 37, 54, 1021, 1022
CseI	GACGC	2 cut(s)	270, 1048
Csp6I	GTAC	7 cut(s)	4, 191, 375, 880, 1232, 1460, 1466
CspCI	CAANNNNNGTGG	4 cut(s)	22, 46, 57, 81
CviAII	CATG	1 cut(s)	1463
CviQI	GTAC	7 cut(s)	4, 191, 375, 880, 1232, 1460, 1466
DdeI	CTNAG	3 cut(s)	23, 97, 219
DpnI	GATC	3 cut(s)	783, 1006, 1082
DpnII	GATC	3 cut(s)	781, 1004, 1080
Eam1104I	CTCTTC	3 cut(s)	147, 558, 819
EarI	CTCTTC	3 cut(s)	147, 558, 819
Ecl136II	GAGCTC	2 cut(s)	132, 568
Eco130I	CCWWGG	1 cut(s)	1248
Eco24I	GRGCYC	3 cut(s)	134, 570, 1025
Eco31I	GGTCTC	1 cut(s)	110
Eco47I	GGWCC	1 cut(s)	27
Eco53kI	GAGCTC	2 cut(s)	132, 568
Eco57I	CTGAAG	1 cut(s)	705
Eco81I	CCTNAGG	1 cut(s)	97
EcoICRI	GAGCTC	2 cut(s)	132, 568
EcoO109I	RGGNCCY	3 cut(s)	27, 1021, 1022
EcoRII	CCWGG	3 cut(s)	166, 612, 1213
EcoT14I	CCWWGG	1 cut(s)	1248
EcoT38I	GRGCYC	3 cut(s)	134, 570, 1025
ErhI	CCWWGG	1 cut(s)	1248
FaeI	CATG	1 cut(s)	1466
FalI	AAGNNNNNCTT	4 cut(s)	951, 983, 1182, 1214
FaqI	GGGAC	1 cut(s)	13
FatI	CATG	1 cut(s)	1462
FauI	CCCGC	1 cut(s)	573
FauNDI	CATATG	1 cut(s)	1447
FbaI	TGATCA	1 cut(s)	781
Fnu4HI	GCNGC	8 cut(s)	12, 78, 279, 712, 768, 1185, 1266, 1489
FokI	GGATG	3 cut(s)	79, 1048, 1241
FriOI	GRGCYC	3 cut(s)	134, 570, 1025
Fsp4HI	GCNGC	8 cut(s)	12, 78, 279, 712, 768, 1185, 1266, 1489
FspBI	CTAG	5 cut(s)	353, 450, 1143, 1283, 1290
GlaI	GCGC	2 cut(s)	947, 1410
GluI	GCNGC	8 cut(s)	12, 78, 279, 712, 768, 1185, 1266, 1489
GsaI	CCCAGC	2 cut(s)	163, 1020
GsuI	CTGGAG	2 cut(s)	171, 965
HaeIII	GGCC	3 cut(s)	39, 56, 1023
HapII	CCGG	7 cut(s)	61, 123, 385, 902, 936, 1026, 1470
HgaI	GACGC	2 cut(s)	270, 1048
HhaI	GCGC	2 cut(s)	948, 1411
Hin1II	CATG	1 cut(s)	1466
Hin6I	GCGC	2 cut(s)	946, 1409
HinP1I	GCGC	2 cut(s)	946, 1409
HincII	GTYRAC	2 cut(s)	175, 520
HindII	GTYRAC	2 cut(s)	175, 520
HpaI	GTTAAC	1 cut(s)	175
HpaII	CCGG	7 cut(s)	61, 123, 385, 902, 936, 1026, 1470
HphI	GGTGA	1 cut(s)	1419
Hpy166II	GTNNAC	3 cut(s)	175, 520, 1257
Hpy188I	TCNGA	6 cut(s)	222, 259, 685, 823, 1156, 1306
Hpy188III	TCNNGA	8 cut(s)	18, 484, 788, 1063, 1076, 1283, 1290, 1363
Hpy8I	GTNNAC	3 cut(s)	175, 520, 1257
HpyAV	CCTTC	2 cut(s)	550, 1311
HpyCH4III	ACNGT	4 cut(s)	624, 884, 976, 1372
HpyCH4IV	ACGT	2 cut(s)	912, 1243
HpyF10VI	GCNNNNNNNGC	6 cut(s)	74, 396, 686, 929, 1045, 1169
HpyF3I	CTNAG	3 cut(s)	23, 97, 219
HpySE526I	ACGT	2 cut(s)	912, 1243
Hsp92II	CATG	1 cut(s)	1466
HspAI	GCGC	2 cut(s)	946, 1409
Ksp22I	TGATCA	1 cut(s)	781
KspAI	GTTAAC	1 cut(s)	175
Kzo9I	GATC	3 cut(s)	781, 1004, 1080
LguI	GCTCTTC	2 cut(s)	147, 558
LmnI	GCTCC	9 cut(s)	168, 387, 437, 454, 721, 754, 850, 937, 1354
Lsp1109I	GCAGC	7 cut(s)	23, 89, 290, 723, 779, 1196, 1500
LweI	GCATC	8 cut(s)	466, 698, 723, 779, 1026, 1116, 1172, 1310
MaeI	CTAG	5 cut(s)	353, 450, 1143, 1283, 1290
MaeII	ACGT	2 cut(s)	912, 1243
MaeIII	GTNAC	3 cut(s)	428, 1222, 1472
MalI	GATC	3 cut(s)	783, 1006, 1082
MboI	GATC	3 cut(s)	781, 1004, 1080
MboII	GAAGA	7 cut(s)	134, 575, 806, 1378, 1388, 1416, 1426
MfeI	CAATTG	1 cut(s)	1203
MflI	RGATCY	2 cut(s)	1004, 1080
MhlI	GDGCHC	5 cut(s)	134, 142, 570, 1025, 1359
MluCI	AATT	7 cut(s)	46, 633, 739, 1157, 1193, 1203, 1482
MlyI	GAGTC	5 cut(s)	15, 280, 515, 1262, 1472
MmeI	TCCRAC	3 cut(s)	132, 606, 850
MroXI	GAANNNNTTC	1 cut(s)	813
MseI	TTAA	5 cut(s)	174, 369, 1113, 1239, 1274
MspA1I	CMGCKG	2 cut(s)	80, 1016
MspI	CCGG	7 cut(s)	61, 123, 385, 902, 936, 1026, 1470
MspR9I	CCNGG	8 cut(s)	61, 124, 168, 614, 936, 1026, 1215, 1470
MunI	CAATTG	1 cut(s)	1203
Mva1269I	GAATGC	1 cut(s)	1097
MvaI	CCWGG	3 cut(s)	168, 614, 1215
MvnI	CGCG	3 cut(s)	948, 1059, 1409
MwoI	GCNNNNNNNGC	6 cut(s)	74, 396, 686, 929, 1045, 1169
NciI	CCSGG	5 cut(s)	61, 124, 936, 1026, 1470
NdeI	CATATG	1 cut(s)	1447
NdeII	GATC	3 cut(s)	781, 1004, 1080
NheI	GCTAGC	1 cut(s)	1142
NlaIII	CATG	1 cut(s)	1466
NmuCI	GTSAC	1 cut(s)	428
NspI	RCATGY	1 cut(s)	1466
PasI	CCCWGGG	1 cut(s)	613
PciI	ACATGT	1 cut(s)	1462
PciSI	GCTCTTC	2 cut(s)	147, 558
PctI	GAATGC	1 cut(s)	1097
PdmI	GAANNNNTTC	1 cut(s)	813
PfeI	GAWTC	7 cut(s)	302, 532, 607, 1066, 1118, 1279, 1286
PflMI	CCANNNNNTGG	1 cut(s)	505
PkrI	GCNGC	8 cut(s)	13, 79, 280, 713, 769, 1186, 1267, 1490
PleI	GAGTC	5 cut(s)	15, 279, 515, 1261, 1472
PpsI	GAGTC	5 cut(s)	15, 279, 515, 1261, 1472
Ppu21I	YACGTR	1 cut(s)	913
PpuMI	RGGWCCY	1 cut(s)	27
PscI	ACATGT	1 cut(s)	1462
PshAI	GACNNNNGTC	1 cut(s)	25
Psp124BI	GAGCTC	2 cut(s)	134, 570
Psp5II	RGGWCCY	1 cut(s)	27
Psp6I	CCWGG	3 cut(s)	166, 612, 1213
PspFI	CCCAGC	2 cut(s)	159, 1016
PspGI	CCWGG	3 cut(s)	166, 612, 1213
PspOMI	GGGCCC	1 cut(s)	1021
PspPI	GGNCC	5 cut(s)	27, 37, 54, 1021, 1022
PspPPI	RGGWCCY	1 cut(s)	27
PstI	CTGCAG	1 cut(s)	280
PstNI	CAGNNNCTG	2 cut(s)	194, 773
PsuI	RGATCY	2 cut(s)	1004, 1080
PvuII	CAGCTG	1 cut(s)	80
RsaI	GTAC	7 cut(s)	5, 192, 376, 881, 1233, 1461, 1467
RsaNI	GTAC	7 cut(s)	4, 191, 375, 880, 1232, 1460, 1466
SacI	GAGCTC	2 cut(s)	134, 570
SapI	GCTCTTC	2 cut(s)	147, 558
SaqAI	TTAA	5 cut(s)	174, 369, 1113, 1239, 1274
SatI	GCNGC	8 cut(s)	12, 78, 279, 712, 768, 1185, 1266, 1489
Sau3AI	GATC	3 cut(s)	781, 1004, 1080
Sau96I	GGNCC	5 cut(s)	27, 37, 54, 1021, 1022
SchI	GAGTC	5 cut(s)	15, 280, 515, 1262, 1472
ScrFI	CCNGG	8 cut(s)	61, 124, 168, 614, 936, 1026, 1215, 1470
SduI	GDGCHC	5 cut(s)	134, 142, 570, 1025, 1359
SfaNI	GCATC	8 cut(s)	466, 698, 723, 779, 1026, 1116, 1172, 1310
SfcI	CTRYAG	1 cut(s)	276
SinI	GGWCC	1 cut(s)	27
SmlI	CTYRAG	3 cut(s)	32, 133, 1042
SmoI	CTYRAG	3 cut(s)	32, 133, 1042
Sse9I	AATT	7 cut(s)	46, 633, 739, 1157, 1193, 1203, 1482
SsiI	CCGC	6 cut(s)	102, 580, 1014, 1057, 1265, 1430
SspMI	CTAG	5 cut(s)	353, 450, 1143, 1283, 1290
SstI	GAGCTC	2 cut(s)	134, 570
StyD4I	CCNGG	8 cut(s)	59, 122, 166, 612, 934, 1024, 1213, 1468
StyI	CCWWGG	1 cut(s)	1248
TaaI	ACNGT	4 cut(s)	624, 884, 976, 1372
TaiI	ACGT	2 cut(s)	915, 1246
TaqI	TCGA	1 cut(s)	86
TasI	AATT	7 cut(s)	46, 633, 739, 1157, 1193, 1203, 1482
TatI	WGTACW	4 cut(s)	3, 374, 1231, 1459
TauI	GCSGC	1 cut(s)	1268
TfiI	GAWTC	7 cut(s)	302, 532, 607, 1066, 1118, 1279, 1286
Tru1I	TTAA	5 cut(s)	174, 369, 1113, 1239, 1274
Tru9I	TTAA	5 cut(s)	174, 369, 1113, 1239, 1274
TscAI	CASTG	4 cut(s)	340, 448, 699, 1153
TseFI	GTSAC	1 cut(s)	428
TseI	GCWGC	7 cut(s)	11, 77, 278, 711, 767, 1184, 1488
Tsp45I	GTSAC	1 cut(s)	428
TspDTI	ATGAA	4 cut(s)	576, 626, 718, 1292
TspGWI	ACGGA	3 cut(s)	138, 1241, 1350
TspRI	CASTG	4 cut(s)	340, 448, 699, 1153
Van91I	CCANNNNNTGG	1 cut(s)	505
VpaK11BI	GGWCC	1 cut(s)	27
XapI	RAATTY	2 cut(s)	46, 1157
XbaI	TCTAGA	2 cut(s)	1282, 1289
XceI	RCATGY	1 cut(s)	1466
XmnI	GAANNNNTTC	1 cut(s)	813
XspI	CTAG	5 cut(s)	353, 450, 1143, 1283, 1290

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.