Rh2CG404100

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002641
Physical Location & Seq
Reverse (-)
55037989 .. 55044197
6209 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG404100.1

Sequence Viewer

Length: 1053 bp
ATGGGATACACTTTTGATGCTCTTAAAACCTATGTTCCTCAGATGGTGGAATTGCTTGTTGACTCTGTGAGAAATCCTGCCTTCTTGGAATGGGAGGTCAATGAGGAGCTGGAAAAGGTGCGGGCAGAAATTGCTGACCTTTCTAAAAATCCTCAAGGCTTACTCTTGGAGAGGATTCATTCGGCTGGTTTTTCTGGAGCTTTGAGAAATCCTCTTTTGGCTTCTGAAGACGCACTGAACAGATTGGATAGCAACATCCTTGAGGAATTTGTTGCTGAGAATTATACTGCTCCTCGGATAGTTCTTGCAGCATCTGGGGTTGACCATGAGGAGCTTTTATCTATTGCAGAACCACTTCTCTCTGACCTACCAAGTGTGCCCCTTGTTGGCGAGCCAAAATCTCAATATGTTGGAGGGGAGTATCGTACTCAAGGTGATACAAAGGATGAGGCACATGTTGCTCTTGCTTTTGAAGTTCCTGGTGGTTGGCGTGAAGAGAAGCAAGCTATCATTTTGACTGTTCTCCAGTTGCTTATGGGAGGAGGTGGCTCTTTCTCTGCCGGGGGTCCTGGAAAGGGGATGCACTCAAGGCTATATCTCCGTGTCTTGAATAATCATCAGGAGATTCAAAATTTTACTGCATTCAACAGCCTCTTTAATGATTCTGGATTGTTTGGAATTTATGCTAGCACTGGCTCTGGGTTTGCATCACAGGCAGTTGAAATAGCAGCAAAAGAGTTACTTTCAATTGCCACACCTGGACAAGTTTCGGATGTAGAGCTTAAACGTGCTAAAGAGTCCACAAAGGCTGCAGTTTTGATGAATCTAGAATCTAGAATGATTGCATCAGAAGATATAGGAAGGCAAGTTTTGACATATGGCGAGAGGAAACCCGTGGAGGATTTCTTAAAGACAGTAGAAGGAGTGACTTTGAAAGATATTACTACAGCCGCCCAACAGATTATATCTTCACCTCTAACAATGGCATCATATGGGAATGTTGTTAATGTCCCAAGCTACGATTCAGTTAACAGAATCTTCCATGGAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006508 GO:0006605 GO:0006626 GO:0006627 GO:0006807 GO:0006810 GO:0006839 GO:0006886 GO:0006950 GO:0006970 GO:0006996 GO:0007005 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010467 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016485 GO:0016787 GO:0017076 GO:0019538 GO:0019866 GO:0019867 GO:0030554 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0034982 GO:0035639 GO:0036094 GO:0042221 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045184 GO:0045275 GO:0046872 GO:0046907 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0051604 GO:0051641 GO:0051649 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0070585 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072655 GO:0097159 GO:0097367 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1902494 GO:1990204
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C

Protein Analysis

350

Amino Acids

38.03

Weight (kDa)

4.88

Isoelectric Point (pI)

37.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 2 - 74 1.1e-12 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 85 - 266 7.4e-32 Peptidase M16 inactive domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 121, 951
AcsI RAATTY 3 cut(s) 266, 631, 678
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 1 cut(s) 427
AfiI CCNNNNNNNGG 2 cut(s) 386, 575
AflIII ACRYGT 1 cut(s) 454
AgsI TTSAA 7 cut(s) 473, 610, 629, 646, 722, 747, 934
AjnI CCWGG 3 cut(s) 478, 568, 757
AjuI GAANNNNNNNTTGG 2 cut(s) 1006, 1038
AluBI AGCT 6 cut(s) 109, 200, 334, 506, 781, 1017
AluI AGCT 6 cut(s) 109, 200, 334, 506, 781, 1017
AlwNI CAGNNNCTG 1 cut(s) 314
ApeKI GCWGC 3 cut(s) 308, 728, 809
ApoI RAATTY 3 cut(s) 266, 631, 678
Asp700I GAANNNNTTC 1 cut(s) 354
AspS9I GGNCC 1 cut(s) 566
AsuC2I CCSGG 1 cut(s) 562
AsuHPI GGTGA 2 cut(s) 446, 963
AsuNHI GCTAGC 1 cut(s) 686
AvaII GGWCC 1 cut(s) 566
BaeGI GKGCMC 1 cut(s) 381
BbsI GAAGAC 1 cut(s) 234
BbvI GCAGC 3 cut(s) 320, 740, 796
BccI CCATC 1 cut(s) 37
BciT130I CCWGG 3 cut(s) 480, 570, 759
BcnI CCSGG 1 cut(s) 562
BfaI CTAG 3 cut(s) 687, 827, 834
BfmI CTRYAG 2 cut(s) 810, 945
BisI GCNGC 4 cut(s) 309, 729, 810, 951
BlsI GCNGC 4 cut(s) 310, 730, 811, 952
Bme1390I CCNGG 4 cut(s) 480, 562, 570, 759
Bme18I GGWCC 1 cut(s) 566
BmgT120I GGNCC 1 cut(s) 566
BmiI GGNNCC 1 cut(s) 567
BmrFI CCNGG 4 cut(s) 480, 562, 570, 759
BmsI GCATC 6 cut(s) 7, 320, 570, 716, 854, 995
BmtI GCTAGC 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 234
BplI GAGNNNNNCTC 2 cut(s) 196, 228
BpmI CTGGAG 2 cut(s) 216, 509
BpuEI CTTGAG 4 cut(s) 138, 281, 414, 571
BpuMI CCSGG 1 cut(s) 562
BsaJI CCNNGG 4 cut(s) 293, 561, 894, 1042
Bsc4I CCNNNNNNNGG 2 cut(s) 386, 575
Bse1I ACTGG 2 cut(s) 526, 697
BseBI CCWGG 3 cut(s) 480, 570, 759
BseDI CCNNGG 4 cut(s) 293, 561, 894, 1042
BseGI GGATG 4 cut(s) 255, 451, 585, 778
BseLI CCNNNNNNNGG 2 cut(s) 386, 575
BseMII CTCAG 2 cut(s) 53, 267
BseNI ACTGG 2 cut(s) 526, 697
BseRI GAGGAG 4 cut(s) 119, 282, 344, 555
BseSI GKGCMC 1 cut(s) 381
BseXI GCAGC 3 cut(s) 320, 740, 796
BsiSI CCGG 1 cut(s) 561
BslFI GGGAC 1 cut(s) 995
BslI CCNNNNNNNGG 2 cut(s) 386, 575
BsmFI GGGAC 1 cut(s) 995
BsmI GAATGC 1 cut(s) 641
Bsp1286I GDGCHC 1 cut(s) 381
Bsp19I CCATGG 1 cut(s) 1042
BspACI CCGC 2 cut(s) 121, 951
BspCNI CTCAG 2 cut(s) 52, 268
BspLI GGNNCC 1 cut(s) 567
BspMAI CTGCAG 1 cut(s) 814
BspOI GCTAGC 1 cut(s) 690
BsrI ACTGG 2 cut(s) 526, 697
BssECI CCNNGG 4 cut(s) 293, 561, 894, 1042
BssT1I CCWWGG 1 cut(s) 1042
Bst2UI CCWGG 3 cut(s) 480, 570, 759
Bst4CI ACNGT 2 cut(s) 520, 916
Bst6I CTCTTC 1 cut(s) 489
BstAPI GCANNNNNTGC 2 cut(s) 131, 458
BstC8I GCNNGC 4 cut(s) 123, 392, 504, 688
BstDEI CTNAG 2 cut(s) 39, 276
BstDSI CCRYGG 2 cut(s) 894, 1042
BstF5I GGATG 4 cut(s) 255, 451, 585, 778
BstMWI GCNNNNNNNGC 4 cut(s) 131, 458, 589, 713
BstNI CCWGG 3 cut(s) 480, 570, 759
BstNSI RCATGY 1 cut(s) 458
BstSCI CCNGG 4 cut(s) 478, 560, 568, 757
BstSFI CTRYAG 2 cut(s) 810, 945
BstSLI GKGCMC 1 cut(s) 381
BstV1I GCAGC 3 cut(s) 320, 740, 796
BstV2I GAAGAC 1 cut(s) 234
BtgI CCRYGG 2 cut(s) 894, 1042
BtsCI GGATG 4 cut(s) 255, 451, 585, 778
BtsIMutI CAGTG 2 cut(s) 233, 690
Cac8I GCNNGC 4 cut(s) 123, 392, 504, 688
CaiI CAGNNNCTG 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 566
CseI GACGC 1 cut(s) 239
Csp6I GTAC 1 cut(s) 426
CviAII CATG 3 cut(s) 326, 455, 1043
CviQI GTAC 1 cut(s) 426
DdeI CTNAG 2 cut(s) 39, 276
Eam1104I CTCTTC 1 cut(s) 489
EarI CTCTTC 1 cut(s) 489
Eco130I CCWWGG 1 cut(s) 1042
Eco47I GGWCC 1 cut(s) 566
Eco57I CTGAAG 1 cut(s) 246
EcoO109I RGGNCCY 1 cut(s) 566
EcoRII CCWGG 3 cut(s) 478, 568, 757
EcoT14I CCWWGG 1 cut(s) 1042
ErhI CCWWGG 1 cut(s) 1042
FaeI CATG 3 cut(s) 329, 458, 1046
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FaqI GGGAC 1 cut(s) 995
FatI CATG 3 cut(s) 325, 454, 1042
FauI CCCGC 1 cut(s) 114
FauNDI CATATG 2 cut(s) 877, 991
Fnu4HI GCNGC 4 cut(s) 309, 729, 810, 951
FokI GGATG 4 cut(s) 242, 458, 592, 785
Fsp4HI GCNGC 4 cut(s) 309, 729, 810, 951
FspBI CTAG 3 cut(s) 687, 827, 834
GluI GCNGC 4 cut(s) 309, 729, 810, 951
GsuI CTGGAG 2 cut(s) 216, 509
HapII CCGG 1 cut(s) 561
HgaI GACGC 1 cut(s) 239
Hin1II CATG 3 cut(s) 329, 458, 1046
HincII GTYRAC 3 cut(s) 61, 322, 1030
HindII GTYRAC 3 cut(s) 61, 322, 1030
HinfI GANTC 9 cut(s) 62, 175, 625, 662, 797, 823, 830, 1022, 1035
HpaI GTTAAC 1 cut(s) 1030
HpaII CCGG 1 cut(s) 561
HphI GGTGA 2 cut(s) 446, 963
Hpy166II GTNNAC 4 cut(s) 61, 322, 801, 1030
Hpy188I TCNGA 6 cut(s) 42, 226, 297, 364, 772, 850
Hpy188III TCNNGA 6 cut(s) 195, 607, 620, 666, 827, 834
Hpy8I GTNNAC 4 cut(s) 61, 322, 801, 1030
HpyAV CCTTC 3 cut(s) 91, 855, 914
HpyCH4III ACNGT 2 cut(s) 520, 916
HpyCH4IV ACGT 1 cut(s) 787
HpyCH4V TGCA 7 cut(s) 308, 347, 583, 641, 707, 812, 845
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 458, 589, 713
HpyF3I CTNAG 2 cut(s) 39, 276
HpySE526I ACGT 1 cut(s) 787
Hsp92II CATG 3 cut(s) 329, 458, 1046
KspAI GTTAAC 1 cut(s) 1030
LmnI GCTCC 4 cut(s) 106, 197, 295, 331
Lsp1109I GCAGC 3 cut(s) 320, 740, 796
LweI GCATC 6 cut(s) 7, 320, 570, 716, 854, 995
MaeI CTAG 3 cut(s) 687, 827, 834
MaeII ACGT 1 cut(s) 787
MaeIII GTNAC 2 cut(s) 738, 925
MboII GAAGA 5 cut(s) 239, 506, 863, 960, 1030
MfeI CAATTG 1 cut(s) 747
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 7 cut(s) 50, 129, 266, 280, 631, 678, 747
MlyI GAGTC 2 cut(s) 56, 806
MmeI TCCRAC 1 cut(s) 391
MroXI GAANNNNTTC 1 cut(s) 354
MseI TTAA 6 cut(s) 24, 657, 783, 908, 1005, 1029
MspI CCGG 1 cut(s) 561
MspR9I CCNGG 4 cut(s) 480, 562, 570, 759
MunI CAATTG 1 cut(s) 747
Mva1269I GAATGC 1 cut(s) 641
MvaI CCWGG 3 cut(s) 480, 570, 759
MwoI GCNNNNNNNGC 4 cut(s) 131, 458, 589, 713
NciI CCSGG 1 cut(s) 562
NcoI CCATGG 1 cut(s) 1042
NdeI CATATG 2 cut(s) 877, 991
NheI GCTAGC 1 cut(s) 686
NlaIII CATG 3 cut(s) 329, 458, 1046
NlaIV GGNNCC 1 cut(s) 567
NmuCI GTSAC 1 cut(s) 925
NspI RCATGY 1 cut(s) 458
PciI ACATGT 1 cut(s) 454
PctI GAATGC 1 cut(s) 641
PdmI GAANNNNTTC 1 cut(s) 354
PfeI GAWTC 7 cut(s) 175, 625, 662, 823, 830, 1022, 1035
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 4 cut(s) 310, 730, 811, 952
PleI GAGTC 2 cut(s) 56, 805
PpsI GAGTC 2 cut(s) 56, 805
PpuMI RGGWCCY 1 cut(s) 566
PscI ACATGT 1 cut(s) 454
Psp5II RGGWCCY 1 cut(s) 566
Psp6I CCWGG 3 cut(s) 478, 568, 757
PspGI CCWGG 3 cut(s) 478, 568, 757
PspN4I GGNNCC 1 cut(s) 567
PspPI GGNCC 1 cut(s) 566
PspPPI RGGWCCY 1 cut(s) 566
PstI CTGCAG 1 cut(s) 814
PstNI CAGNNNCTG 1 cut(s) 314
RsaI GTAC 1 cut(s) 427
RsaNI GTAC 1 cut(s) 426
SaqAI TTAA 6 cut(s) 24, 657, 783, 908, 1005, 1029
SatI GCNGC 4 cut(s) 309, 729, 810, 951
Sau96I GGNCC 1 cut(s) 566
SchI GAGTC 2 cut(s) 56, 806
ScrFI CCNGG 4 cut(s) 480, 562, 570, 759
SduI GDGCHC 1 cut(s) 381
SfaNI GCATC 6 cut(s) 7, 320, 570, 716, 854, 995
SfcI CTRYAG 2 cut(s) 810, 945
SinI GGWCC 1 cut(s) 566
SmlI CTYRAG 4 cut(s) 153, 260, 429, 586
SmoI CTYRAG 4 cut(s) 153, 260, 429, 586
Sse9I AATT 7 cut(s) 50, 129, 266, 280, 631, 678, 747
SsiI CCGC 2 cut(s) 121, 951
SspMI CTAG 3 cut(s) 687, 827, 834
StyD4I CCNGG 4 cut(s) 478, 560, 568, 757
StyI CCWWGG 1 cut(s) 1042
TaaI ACNGT 2 cut(s) 520, 916
TaiI ACGT 1 cut(s) 790
TasI AATT 7 cut(s) 50, 129, 266, 280, 631, 678, 747
TauI GCSGC 1 cut(s) 953
TfiI GAWTC 7 cut(s) 175, 625, 662, 823, 830, 1022, 1035
Tru1I TTAA 6 cut(s) 24, 657, 783, 908, 1005, 1029
Tru9I TTAA 6 cut(s) 24, 657, 783, 908, 1005, 1029
TscAI CASTG 2 cut(s) 240, 697
TseFI GTSAC 1 cut(s) 925
TseI GCWGC 3 cut(s) 308, 728, 809
Tsp45I GTSAC 1 cut(s) 925
TspDTI ATGAA 2 cut(s) 167, 836
TspGWI ACGGA 1 cut(s) 590
TspRI CASTG 2 cut(s) 240, 697
VpaK11BI GGWCC 1 cut(s) 566
XapI RAATTY 3 cut(s) 266, 631, 678
XbaI TCTAGA 2 cut(s) 826, 833
XceI RCATGY 1 cut(s) 458
XmnI GAANNNNTTC 1 cut(s) 354
XspI CTAG 3 cut(s) 687, 827, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.