Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0002641

Physical Location & Seq

Genomic Coordinates Reverse (-)

58440127 .. 58445373

Gene Structure 5247 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000019911

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1692 bp

ATGAGAACAAGCTTACCAATACTCGATCGGAGCAGAAATTTTCGAGATACCCAATCAGAAAACGAACACCAATTTTCATATATTGGGCTTTTTGTCGAGCTGCCTCTCTCTCGTAAAGCTCAAAGCTTCAACCAAACAAACGCAGAGACTGACTTTCTAGGGCTTCAATCTTCCATCAACATGTACAGAACTGCAGCTTCGCGAGTCAGGTCGCTTAAGGGCCGTGTGGGCGCCACCAGGTATGCGTCAGTAACTGCAGCTGCTACAAAGCCAGCCTCAGGGGGTATCTTTAGCTGGCTGACTGGGGAAAAGTCCAGTGCTCTTCCTCGTCTGGAAACCCCATTAGCAGGTGTGAACCTCCCTCCTCCACTACCTGATTATGTGGAACCAAGCAAGACTAAGGTTACAAAGCTCTCCAATGGTGTCAGAATAGCTTCAGAAAAGTCACCGAGTCCTGCAGCCTCCATTGGATTATATGTTAATTGTGGTTCCATTTATGAGACTCCAGTAACGAGTGGGGCTTCACACTTGTTAGAAAGGCTGGCCTTTAAGAGCACAACCAACCGGAGCCACTTGCGCATTGTTAGAGAAGTGGAAGCAATTGGTGGTAACATTGCAGCCAGTGCCTCTAGGGAACAAATGGGATACACTTTCGATGCTCTTAAAACCTATGTTCCTCAGATGGTGGAATTGCTTGTTGACTCTGTGAGAAATCCTGCCTTCTTGGAATGGGAAGTCAATGAGGAGCTGGAAAAGGTGCGGGCAGAAATTGCAGAACTTTCTAAGAATCCTCAAGGCTTGCTCTTGGAGAGGATTCATTCGGCTGGTTTTTCTGGAGCATTAAGAAATCCTCTTTTGGCTTCTGAAGATGCACTGAACAGATTGGATAGCAACATCCTTGAGGAATTTGTTGCTGAGAATTATACTGCTCCTCGGATAGTTCTTGCAGCATCTGGGGTTGATCATGAGGAGCTTTTATCTATTGCAGAACCTCTTCTCTCTGACCTACCAAGTGTGCCCCTTGTTGGCGAGCCAAAATCTCAATATGTTGGAGGGGAGTATCGTACTCAAGGTGATACAAAGGATGAGGCACATATTGCTCTTGCTTTTGAAGTTCCTGGTGGTTGGCGTCAAGAGAAGCAAGCTATCATTTTGACTGTTCTCCAGTTGCTTATGGGAGGAGGTGGCTCTTTCTCTGCCGGGGGTCCTGGAAAGGGGATGCACTCAAGGCTATATCTCCGTGTCTTGAATAATCATCAGGAGATTCAAAATTTTACTGCATTCAACAGCCTCTTTAATGATTCTGGATTGTTTGGAATTTATGCTAGCACTGGCTCTGGGTTTGCATCACAGGCAGTTGAAATAGCAGCAAAAGAGTTGCTTTCAATTGCCACACCTGGACAAGTTTCGGATGTAGAGCTTAAACGTGCTAAAGAGTCCACAAAGGCTGCAGTTTTGATGAATCTAGAATCTAGAATGATTGCATCAGAAGATATAGGAAGGCAAGTTTTGACATATGGAGAGAGGAAACCTGTGGAGGATTTCTTAAAGACAGTAGAAGGAGTGACTTTGAAAGATATTACTACAGCCGCCCAACAGATTATATCTTCACCTCTAACAATGGCATCATATGGGAATGTTGTAAATGTCCCAAGCTACGATTCAGTTAACAGAATCTTCCATGCAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006508 GO:0006605 GO:0006626 GO:0006627 GO:0006807 GO:0006810 GO:0006839 GO:0006886 GO:0006950 GO:0006970 GO:0006996 GO:0007005 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010467 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016485 GO:0016787 GO:0017076 GO:0019538 GO:0019866 GO:0019867 GO:0030554 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0034982 GO:0035639 GO:0036094 GO:0042221 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045184 GO:0045275 GO:0046872 GO:0046907 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0051604 GO:0051641 GO:0051649 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0070585 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072655 GO:0097159 GO:0097367 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1902494 GO:1990204

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M16 Peptidase_M16_C

Protein Analysis

564

Amino Acids

61.09

Weight (kDa)

5.97

Isoelectric Point (pI)

40.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M16	PF00675	143 - 287	7.3e-42	Insulinase (Peptidase family M16)
Peptidase_M16_C	PF05193	298 - 479	1.3e-31	Peptidase M16 inactive domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002641)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G51980 AT1G51980 AT3G16480
fragaria_vesca	FvH4_1g06540 FvH4_1g06540 FvH4_1g06540 FvH4_6g32600 FvH4_6g32600
malus_domestica	MD02G1068800.v1.1 MD09G1200100.v1.1 MD17G1181200.v1.1
prunus_persica	Prupe.3G056700_v2.0.a1 Prupe.7G216900_v2.0.a1
pyrus_communis	pycom02g05450 pycom09g11770 pycom17g19060
rosa_chinensis	RchiOBHm_Chr2g0092291 RchiOBHm_Chr2g0141051
rosa_laevigata	RLG00000016294 RLG00000019911
rosa_multiflora	Rmu_co8416335.1_g000001 Rmu_sc0003289.1_g000021 Rmu_sc0004769.1_g000017 Rmu_sc0012897.1_g000005
rosa_roxburghii	Rroxscaffold_2G00104250 Rroxscaffold_2G00149290
rosa_rugosa	Rorug02G0024700 Rorug02G0366400
rosa_samantha	Rh2AG070400 Rh2AG417800 Rh2BG069900 Rh2BG427700 Rh2CG071100 Rh2CG356200 Rh2CG404100 Rh2DG068900 Rh2DG437200
rosa_wichuraiana	Rw2G006190 Rw2G034200 Rw2G034260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	338
Acc16I	TGCGCA	1 cut(s)	578
Acc36I	ACCTGC	1 cut(s)	338
AccB1I	GGYRCC	1 cut(s)	230
AccII	CGCG	1 cut(s)	202
AciI	CCGC	2 cut(s)	760, 1590
AcsI	RAATTY	4 cut(s)	37, 905, 1270, 1317
AcuI	CTGAAG	2 cut(s)	420, 885
AcyI	GRCGYC	2 cut(s)	231, 1129
AfaI	GTAC	2 cut(s)	185, 1066
AfiI	CCNNNNNNNGG	4 cut(s)	278, 347, 1025, 1214
AflII	CTTAAG	1 cut(s)	215
AflIII	ACRYGT	1 cut(s)	180
AgsI	TTSAA	9 cut(s)	130, 167, 1112, 1249, 1268, 1285, 1361, 1386, 1573
AjnI	CCWGG	4 cut(s)	236, 1117, 1207, 1396
AjuI	GAANNNNNNNTTGG	2 cut(s)	1645, 1677
Alw21I	GWGCWC	2 cut(s)	322, 557
Alw26I	GTCTC	2 cut(s)	140, 494
AlwNI	CAGNNNCTG	3 cut(s)	149, 254, 953
AoxI	GGCC	2 cut(s)	220, 543
ApeKI	GCWGC	9 cut(s)	100, 194, 257, 260, 458, 617, 947, 1367, 1448
ApoI	RAATTY	4 cut(s)	37, 905, 1270, 1317
Asp700I	GAANNNNTTC	2 cut(s)	433, 993
AspLEI	GCGC	2 cut(s)	233, 579
AspS9I	GGNCC	2 cut(s)	220, 1205
AsuC2I	CCSGG	1 cut(s)	1201
AsuHPI	GGTGA	3 cut(s)	438, 1085, 1602
AsuNHI	GCTAGC	1 cut(s)	1325
AvaII	GGWCC	1 cut(s)	1205
AxyI	CCTNAGG	1 cut(s)	277
BaeGI	GKGCMC	1 cut(s)	1020
BanI	GGYRCC	1 cut(s)	230
Bbv12I	GWGCWC	2 cut(s)	322, 557
BbvI	GCAGC	9 cut(s)	87, 206, 247, 269, 470, 629, 959, 1379, 1435
BccI	CCATC	2 cut(s)	182, 676
BceAI	ACGGC	1 cut(s)	207
BciT130I	CCWGG	4 cut(s)	238, 1119, 1209, 1398
BciVI	GTATCC	1 cut(s)	638
BclI	TGATCA	1 cut(s)	961
BcnI	CCSGG	1 cut(s)	1201
BcoDI	GTCTC	2 cut(s)	140, 494
BfaI	CTAG	5 cut(s)	158, 630, 1326, 1466, 1473
BfmI	CTRYAG	5 cut(s)	192, 255, 456, 1449, 1584
BfoI	RGCGCY	1 cut(s)	234
BfrI	CTTAAG	1 cut(s)	215
BfuAI	ACCTGC	1 cut(s)	338
BfuI	GTATCC	1 cut(s)	638
BglI	GCCNNNNNGGC	1 cut(s)	228
Bme1390I	CCNGG	5 cut(s)	238, 1119, 1201, 1209, 1398
Bme18I	GGWCC	1 cut(s)	1205
BmgT120I	GGNCC	2 cut(s)	220, 1205
BmiI	GGNNCC	5 cut(s)	232, 387, 490, 569, 1206
BmrFI	CCNGG	5 cut(s)	238, 1119, 1201, 1209, 1398
BmrI	ACTGGG	1 cut(s)	312
BmsI	GCATC	7 cut(s)	646, 859, 959, 1209, 1355, 1493, 1634
BmtI	GCTAGC	1 cut(s)	1329
BmuI	ACTGGG	1 cut(s)	312
BpmI	CTGGAG	3 cut(s)	489, 855, 1148
BpuEI	CTTGAG	4 cut(s)	777, 920, 1053, 1210
BpuMI	CCSGG	1 cut(s)	1201
BsaHI	GRCGYC	2 cut(s)	231, 1129
BsaJI	CCNNGG	2 cut(s)	932, 1200
BsaWI	WCCGGW	1 cut(s)	564
Bsc4I	CCNNNNNNNGG	4 cut(s)	278, 347, 1025, 1214
Bse1I	ACTGG	6 cut(s)	307, 315, 506, 621, 1165, 1336
Bse21I	CCTNAGG	1 cut(s)	277
Bse3DI	GCAATG	1 cut(s)	612
BseBI	CCWGG	4 cut(s)	238, 1119, 1209, 1398
BseDI	CCNNGG	2 cut(s)	932, 1200
BseGI	GGATG	4 cut(s)	894, 1090, 1224, 1417
BseLI	CCNNNNNNNGG	4 cut(s)	278, 347, 1025, 1214
BseMI	GCAATG	1 cut(s)	612
BseMII	CTCAG	3 cut(s)	291, 692, 906
BseNI	ACTGG	6 cut(s)	307, 315, 506, 621, 1165, 1336
BseRI	GAGGAG	5 cut(s)	354, 758, 921, 983, 1194
BseSI	GKGCMC	1 cut(s)	1020
BseXI	GCAGC	9 cut(s)	87, 206, 247, 269, 470, 629, 959, 1379, 1435
Bsh1236I	CGCG	1 cut(s)	202
Bsh1285I	CGRYCG	1 cut(s)	28
BshFI	GGCC	2 cut(s)	222, 545
BshNI	GGYRCC	1 cut(s)	230
BsiEI	CGRYCG	1 cut(s)	28
BsiHKAI	GWGCWC	2 cut(s)	322, 557
BsiSI	CCGG	2 cut(s)	565, 1200
BslFI	GGGAC	1 cut(s)	1634
BslI	CCNNNNNNNGG	4 cut(s)	278, 347, 1025, 1214
BsmAI	GTCTC	2 cut(s)	140, 494
BsmFI	GGGAC	1 cut(s)	1634
BsmI	GAATGC	1 cut(s)	1280
BsnI	GGCC	2 cut(s)	222, 545
Bsp1286I	GDGCHC	3 cut(s)	322, 557, 1020
Bsp1407I	TGTACA	1 cut(s)	183
Bsp143I	GATC	2 cut(s)	25, 961
Bsp68I	TCGCGA	1 cut(s)	202
BspACI	CCGC	2 cut(s)	760, 1590
BspANI	GGCC	2 cut(s)	222, 545
BspCNI	CTCAG	3 cut(s)	290, 691, 907
BspFNI	CGCG	1 cut(s)	202
BspHI	TCATGA	1 cut(s)	964
BspLI	GGNNCC	5 cut(s)	232, 387, 490, 569, 1206
BspMAI	CTGCAG	4 cut(s)	196, 259, 460, 1453
BspMI	ACCTGC	1 cut(s)	338
BspOI	GCTAGC	1 cut(s)	1329
BspQI	GCTCTTC	1 cut(s)	327
BspT107I	GGYRCC	1 cut(s)	230
BspTI	CTTAAG	1 cut(s)	215
BsrDI	GCAATG	1 cut(s)	612
BsrGI	TGTACA	1 cut(s)	183
BsrI	ACTGG	6 cut(s)	307, 315, 506, 621, 1165, 1336
BssECI	CCNNGG	2 cut(s)	932, 1200
BssMI	GATC	2 cut(s)	25, 961
BssNI	GRCGYC	2 cut(s)	231, 1129
Bst2UI	CCWGG	4 cut(s)	238, 1119, 1209, 1398
Bst4CI	ACNGT	2 cut(s)	1159, 1555
Bst6I	CTCTTC	2 cut(s)	327, 999
BstACI	GRCGYC	2 cut(s)	231, 1129
BstAFI	CTTAAG	1 cut(s)	215
BstAPI	GCANNNNNTGC	3 cut(s)	623, 770, 1097
BstAUI	TGTACA	1 cut(s)	183
BstC8I	GCNNGC	8 cut(s)	273, 296, 543, 762, 800, 1031, 1143, 1327
BstDEI	CTNAG	5 cut(s)	277, 399, 678, 783, 915
BstF5I	GGATG	4 cut(s)	894, 1090, 1224, 1417
BstFNI	CGCG	1 cut(s)	202
BstH2I	RGCGCY	1 cut(s)	234
BstHHI	GCGC	2 cut(s)	233, 579
BstKTI	GATC	2 cut(s)	28, 964
BstMAI	GTCTC	2 cut(s)	140, 494
BstMBI	GATC	2 cut(s)	25, 961
BstMCI	CGRYCG	1 cut(s)	28
BstMWI	GCNNNNNNNGC	7 cut(s)	228, 576, 623, 770, 1097, 1228, 1352
BstNI	CCWGG	4 cut(s)	238, 1119, 1209, 1398
BstNSI	RCATGY	1 cut(s)	184
BstSCI	CCNGG	5 cut(s)	236, 1117, 1199, 1207, 1396
BstSFI	CTRYAG	5 cut(s)	192, 255, 456, 1449, 1584
BstSLI	GKGCMC	1 cut(s)	1020
BstUI	CGCG	1 cut(s)	202
BstV1I	GCAGC	9 cut(s)	87, 206, 247, 269, 470, 629, 959, 1379, 1435
Bsu36I	CCTNAGG	1 cut(s)	277
BsuI	GTATCC	1 cut(s)	638
BsuRI	GGCC	2 cut(s)	222, 545
BtsCI	GGATG	4 cut(s)	894, 1090, 1224, 1417
BtsIMutI	CAGTG	4 cut(s)	322, 628, 872, 1329
BtuMI	TCGCGA	1 cut(s)	202
BveI	ACCTGC	1 cut(s)	338
Cac8I	GCNNGC	8 cut(s)	273, 296, 543, 762, 800, 1031, 1143, 1327
CaiI	CAGNNNCTG	3 cut(s)	149, 254, 953
CciI	TCATGA	1 cut(s)	964
CfoI	GCGC	2 cut(s)	233, 579
Cfr13I	GGNCC	2 cut(s)	220, 1205
CseI	GACGC	2 cut(s)	234, 1118
CsiI	ACCWGGT	1 cut(s)	236
Csp6I	GTAC	2 cut(s)	184, 1065
CviAII	CATG	3 cut(s)	181, 965, 1682
CviQI	GTAC	2 cut(s)	184, 1065
DdeI	CTNAG	5 cut(s)	277, 399, 678, 783, 915
DinI	GGCGCC	1 cut(s)	232
DpnI	GATC	2 cut(s)	27, 963
DpnII	GATC	2 cut(s)	25, 961
Eam1104I	CTCTTC	2 cut(s)	327, 999
EarI	CTCTTC	2 cut(s)	327, 999
Eco47I	GGWCC	1 cut(s)	1205
Eco57I	CTGAAG	2 cut(s)	420, 885
Eco81I	CCTNAGG	1 cut(s)	277
EcoO109I	RGGNCCY	1 cut(s)	1205
EcoRII	CCWGG	4 cut(s)	236, 1117, 1207, 1396
EgeI	GGCGCC	1 cut(s)	232
EheI	GGCGCC	1 cut(s)	232
FaeI	CATG	3 cut(s)	184, 968, 1685
FalI	AAGNNNNNCTT	2 cut(s)	1365, 1397
FaqI	GGGAC	1 cut(s)	1634
FatI	CATG	3 cut(s)	180, 964, 1681
FauI	CCCGC	1 cut(s)	753
FauNDI	CATATG	2 cut(s)	1516, 1630
FbaI	TGATCA	1 cut(s)	961
FokI	GGATG	4 cut(s)	881, 1097, 1231, 1424
FspBI	CTAG	5 cut(s)	158, 630, 1326, 1466, 1473
FspI	TGCGCA	1 cut(s)	578
GlaI	GCGC	2 cut(s)	232, 578
GsuI	CTGGAG	3 cut(s)	489, 855, 1148
HaeII	RGCGCY	1 cut(s)	234
HaeIII	GGCC	2 cut(s)	222, 545
HapII	CCGG	2 cut(s)	565, 1200
HgaI	GACGC	2 cut(s)	234, 1118
HhaI	GCGC	2 cut(s)	233, 579
Hin1I	GRCGYC	2 cut(s)	231, 1129
Hin1II	CATG	3 cut(s)	184, 968, 1685
Hin6I	GCGC	2 cut(s)	231, 577
HinP1I	GCGC	2 cut(s)	231, 577
HincII	GTYRAC	2 cut(s)	700, 1669
HindII	GTYRAC	2 cut(s)	700, 1669
HindIII	AAGCTT	2 cut(s)	10, 124
HpaI	GTTAAC	1 cut(s)	1669
HpaII	CCGG	2 cut(s)	565, 1200
HphI	GGTGA	3 cut(s)	438, 1085, 1602
Hpy166II	GTNNAC	4 cut(s)	355, 700, 1440, 1669
Hpy8I	GTNNAC	4 cut(s)	355, 700, 1440, 1669
HpyAV	CCTTC	3 cut(s)	730, 1494, 1553
HpyCH4III	ACNGT	2 cut(s)	1159, 1555
HpyCH4IV	ACGT	1 cut(s)	1426
HpyF10VI	GCNNNNNNNGC	7 cut(s)	228, 576, 623, 770, 1097, 1228, 1352
HpyF3I	CTNAG	5 cut(s)	277, 399, 678, 783, 915
HpySE526I	ACGT	1 cut(s)	1426
Hsp92I	GRCGYC	2 cut(s)	231, 1129
Hsp92II	CATG	3 cut(s)	184, 968, 1685
HspAI	GCGC	2 cut(s)	231, 577
KasI	GGCGCC	1 cut(s)	230
Ksp22I	TGATCA	1 cut(s)	961
KspAI	GTTAAC	1 cut(s)	1669
Kzo9I	GATC	2 cut(s)	25, 961
LguI	GCTCTTC	1 cut(s)	327
LmnI	GCTCC	6 cut(s)	30, 567, 745, 836, 934, 970
Lsp1109I	GCAGC	9 cut(s)	87, 206, 247, 269, 470, 629, 959, 1379, 1435
LweI	GCATC	7 cut(s)	646, 859, 959, 1209, 1355, 1493, 1634
MabI	ACCWGGT	1 cut(s)	236
MaeI	CTAG	5 cut(s)	158, 630, 1326, 1466, 1473
MaeII	ACGT	1 cut(s)	1426
MaeIII	GTNAC	6 cut(s)	250, 403, 444, 508, 608, 1564
MalI	GATC	2 cut(s)	27, 963
MboI	GATC	2 cut(s)	25, 961
MboII	GAAGA	7 cut(s)	162, 314, 878, 986, 1502, 1599, 1669
MfeI	CAATTG	2 cut(s)	600, 1386
MhlI	GDGCHC	3 cut(s)	322, 557, 1020
Mly113I	GGCGCC	1 cut(s)	231
MlyI	GAGTC	5 cut(s)	213, 460, 496, 695, 1445
MmeI	TCCRAC	1 cut(s)	1030
MroXI	GAANNNNTTC	2 cut(s)	433, 993
MseI	TTAA	9 cut(s)	216, 480, 549, 663, 842, 1296, 1422, 1547, 1668
MslI	CAYNNNNRTG	1 cut(s)	179
MspA1I	CMGCKG	1 cut(s)	260
MspCI	CTTAAG	1 cut(s)	215
MspI	CCGG	2 cut(s)	565, 1200
MspR9I	CCNGG	5 cut(s)	238, 1119, 1201, 1209, 1398
MunI	CAATTG	2 cut(s)	600, 1386
Mva1269I	GAATGC	1 cut(s)	1280
MvaI	CCWGG	4 cut(s)	238, 1119, 1209, 1398
MvnI	CGCG	1 cut(s)	202
MwoI	GCNNNNNNNGC	7 cut(s)	228, 576, 623, 770, 1097, 1228, 1352
NarI	GGCGCC	1 cut(s)	231
NciI	CCSGG	1 cut(s)	1201
NdeI	CATATG	2 cut(s)	1516, 1630
NdeII	GATC	2 cut(s)	25, 961
NheI	GCTAGC	1 cut(s)	1325
NlaIII	CATG	3 cut(s)	184, 968, 1685
NlaIV	GGNNCC	5 cut(s)	232, 387, 490, 569, 1206
NmuCI	GTSAC	2 cut(s)	444, 1564
NruI	TCGCGA	1 cut(s)	202
NsbI	TGCGCA	1 cut(s)	578
NspI	RCATGY	1 cut(s)	184
PagI	TCATGA	1 cut(s)	964
PaqCI	CACCTGC	1 cut(s)	338
PciI	ACATGT	1 cut(s)	180
PciSI	GCTCTTC	1 cut(s)	327
PctI	GAATGC	1 cut(s)	1280
PdmI	GAANNNNTTC	2 cut(s)	433, 993
PfeI	GAWTC	8 cut(s)	787, 814, 1264, 1301, 1462, 1469, 1661, 1674
PfoI	TCCNGGA	1 cut(s)	1207
Ple19I	CGATCG	1 cut(s)	28
PleI	GAGTC	5 cut(s)	212, 459, 496, 695, 1444
PluTI	GGCGCC	1 cut(s)	234
PpsI	GAGTC	5 cut(s)	212, 459, 496, 695, 1444
PpuMI	RGGWCCY	1 cut(s)	1205
PscI	ACATGT	1 cut(s)	180
Psp5II	RGGWCCY	1 cut(s)	1205
Psp6I	CCWGG	4 cut(s)	236, 1117, 1207, 1396
PspGI	CCWGG	4 cut(s)	236, 1117, 1207, 1396
PspN4I	GGNNCC	5 cut(s)	232, 387, 490, 569, 1206
PspPI	GGNCC	2 cut(s)	220, 1205
PspPPI	RGGWCCY	1 cut(s)	1205
PstI	CTGCAG	4 cut(s)	196, 259, 460, 1453
PstNI	CAGNNNCTG	3 cut(s)	149, 254, 953
PvuI	CGATCG	1 cut(s)	28
PvuII	CAGCTG	1 cut(s)	260
RruI	TCGCGA	1 cut(s)	202
RsaI	GTAC	2 cut(s)	185, 1066
RsaNI	GTAC	2 cut(s)	184, 1065
RseI	CAYNNNNRTG	1 cut(s)	179
SapI	GCTCTTC	1 cut(s)	327
SaqAI	TTAA	9 cut(s)	216, 480, 549, 663, 842, 1296, 1422, 1547, 1668
Sau3AI	GATC	2 cut(s)	25, 961
Sau96I	GGNCC	2 cut(s)	220, 1205
SchI	GAGTC	5 cut(s)	213, 460, 496, 695, 1445
ScrFI	CCNGG	5 cut(s)	238, 1119, 1201, 1209, 1398
SduI	GDGCHC	3 cut(s)	322, 557, 1020
SexAI	ACCWGGT	1 cut(s)	236
SfaNI	GCATC	7 cut(s)	646, 859, 959, 1209, 1355, 1493, 1634
SfcI	CTRYAG	5 cut(s)	192, 255, 456, 1449, 1584
SfoI	GGCGCC	1 cut(s)	232
SinI	GGWCC	1 cut(s)	1205
SmiMI	CAYNNNNRTG	1 cut(s)	179
SmlI	CTYRAG	5 cut(s)	215, 792, 899, 1068, 1225
SmoI	CTYRAG	5 cut(s)	215, 792, 899, 1068, 1225
SsiI	CCGC	2 cut(s)	760, 1590
SspDI	GGCGCC	1 cut(s)	230
SspMI	CTAG	5 cut(s)	158, 630, 1326, 1466, 1473
StyD4I	CCNGG	5 cut(s)	236, 1117, 1199, 1207, 1396
TaaI	ACNGT	2 cut(s)	1159, 1555
TaiI	ACGT	1 cut(s)	1429
TaqI	TCGA	4 cut(s)	24, 43, 96, 654
TatI	WGTACW	1 cut(s)	183
TauI	GCSGC	1 cut(s)	1592
TfiI	GAWTC	8 cut(s)	787, 814, 1264, 1301, 1462, 1469, 1661, 1674
Tru1I	TTAA	9 cut(s)	216, 480, 549, 663, 842, 1296, 1422, 1547, 1668
Tru9I	TTAA	9 cut(s)	216, 480, 549, 663, 842, 1296, 1422, 1547, 1668
TscAI	CASTG	4 cut(s)	322, 628, 879, 1336
TseFI	GTSAC	2 cut(s)	444, 1564
TseI	GCWGC	9 cut(s)	100, 194, 257, 260, 458, 617, 947, 1367, 1448
Tsp45I	GTSAC	2 cut(s)	444, 1564
TspDTI	ATGAA	3 cut(s)	66, 806, 1475
TspGWI	ACGGA	1 cut(s)	1229
TspRI	CASTG	4 cut(s)	322, 628, 879, 1336
Vha464I	CTTAAG	1 cut(s)	215
VpaK11BI	GGWCC	1 cut(s)	1205
XapI	RAATTY	4 cut(s)	37, 905, 1270, 1317
XbaI	TCTAGA	2 cut(s)	1465, 1472
XceI	RCATGY	1 cut(s)	184
XmnI	GAANNNNTTC	2 cut(s)	433, 993
XspI	CTAG	5 cut(s)	158, 630, 1326, 1466, 1473

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.