Rh2CG356200

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002641
Physical Location & Seq
Forward (+)
48250293 .. 48263757
13465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG356200.1

Sequence Viewer

Length: 576 bp
ATGAACTCGCGATTATATATTCGTGTGTTGGCTCAAAATCCCGAGGTTAAGTCAATGTCAGTAATCAATAGCATCTACAACAATAGTGCTTTATTTGGCATCCAAGCTTCCACTAGTTCAGATTATGTGACAGAAGCCATTGATATAGCAGCAGATGAGCTTATTGCACTTGCAACCCCTGGAGAAGTTGACCTGGTACAGCTAGATCGTGCCAAACAGTCAACGAAGTCTTCCATTCTTATGAATTTGGAATCTAGAGCATGTAAGAAGAATTTGGTTACTGTTCTTATACAGAAATTGAAGGAAATGCTTCCTCTGACATGGGTGTTGCTGAGGACTGCCGCAATACATTCTTGGAACTGCAGAGGTTGTGGAGAAGAAGAAAGAGAAAAGATCAGCCAGAGGATGAAAATTCTTCAGGATTTGGTTCCTGGATGTAATAAGATTGAGTTGAACCACACAGGCACAACCCTGATGCATAAAGTAGCCAACGGTAAAGAAAGAGCTGCTCTAGCACAATGGACAATGCTATTGCAAATTATAAGTACACAACTTCAAGTGACTGAAGGCAACTGA

Protein Analysis

191

Amino Acids

21.25

Weight (kDa)

8.35

Isoelectric Point (pI)

37.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16_C PF05193 1 - 73 6e-06 Peptidase M16 inactive domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 542
AccII CGCG 1 cut(s) 10
AciI CCGC 1 cut(s) 342
AcsI RAATTY 3 cut(s) 244, 271, 411
AcuI CTGAAG 1 cut(s) 401
AfaI GTAC 2 cut(s) 198, 547
AgsI TTSAA 3 cut(s) 301, 454, 557
AhlI ACTAGT 1 cut(s) 113
AjnI CCWGG 3 cut(s) 178, 192, 430
AluBI AGCT 4 cut(s) 107, 160, 202, 506
AluI AGCT 4 cut(s) 107, 160, 202, 506
Ama87I CYCGRG 1 cut(s) 41
ApeKI GCWGC 2 cut(s) 149, 506
ApoI RAATTY 3 cut(s) 244, 271, 411
Asp700I GAANNNNTTC 1 cut(s) 309
AvaI CYCGRG 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 222
BbvCI CCTCAGC 1 cut(s) 332
BbvI GCAGC 2 cut(s) 161, 493
BciT130I CCWGG 3 cut(s) 180, 194, 432
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 4 cut(s) 114, 203, 255, 512
BfmI CTRYAG 1 cut(s) 361
BisI GCNGC 3 cut(s) 150, 342, 507
BlsI GCNGC 3 cut(s) 151, 343, 508
Bme1390I CCNGG 3 cut(s) 180, 194, 432
BmeT110I CYCGRG 1 cut(s) 41
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 3 cut(s) 180, 194, 432
BmsI GCATC 3 cut(s) 81, 108, 465
BpiI GAAGAC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 201
Bpu10I CCTNAGC 1 cut(s) 332
BsaJI CCNNGG 2 cut(s) 42, 178
BseBI CCWGG 3 cut(s) 180, 194, 432
BseDI CCNNGG 2 cut(s) 42, 178
BseGI GGATG 3 cut(s) 99, 411, 440
BseMII CTCAG 1 cut(s) 323
BseXI GCAGC 2 cut(s) 161, 493
Bsh1236I CGCG 1 cut(s) 10
BsiHKCI CYCGRG 1 cut(s) 41
BsoBI CYCGRG 1 cut(s) 41
Bsp143I GATC 2 cut(s) 205, 393
Bsp68I TCGCGA 1 cut(s) 10
BspACI CCGC 1 cut(s) 342
BspCNI CTCAG 1 cut(s) 324
BspFNI CGCG 1 cut(s) 10
BspLI GGNNCC 1 cut(s) 429
BspMAI CTGCAG 1 cut(s) 365
BssECI CCNNGG 2 cut(s) 42, 178
BssMI GATC 2 cut(s) 205, 393
Bst2UI CCWGG 3 cut(s) 180, 194, 432
Bst4CI ACNGT 3 cut(s) 219, 283, 494
BstDEI CTNAG 1 cut(s) 332
BstF5I GGATG 3 cut(s) 99, 411, 440
BstFNI CGCG 1 cut(s) 10
BstKTI GATC 2 cut(s) 208, 396
BstMBI GATC 2 cut(s) 205, 393
BstMWI GCNNNNNNNGC 1 cut(s) 512
BstNI CCWGG 3 cut(s) 180, 194, 432
BstNSI RCATGY 1 cut(s) 264
BstSCI CCNGG 3 cut(s) 178, 192, 430
BstSFI CTRYAG 1 cut(s) 361
BstUI CGCG 1 cut(s) 10
BstV1I GCAGC 2 cut(s) 161, 493
BstV2I GAAGAC 1 cut(s) 222
BtsCI GGATG 3 cut(s) 99, 411, 440
BtuMI TCGCGA 1 cut(s) 10
CsiI ACCWGGT 1 cut(s) 192
Csp6I GTAC 2 cut(s) 197, 546
CviAII CATG 2 cut(s) 261, 321
CviJI RGCY 8 cut(s) 32, 107, 137, 160, 202, 399, 488, 506
CviKI_1 RGCY 8 cut(s) 32, 107, 137, 160, 202, 399, 488, 506
CviQI GTAC 2 cut(s) 197, 546
DdeI CTNAG 1 cut(s) 332
DpnI GATC 2 cut(s) 207, 395
DpnII GATC 2 cut(s) 205, 393
Eco57I CTGAAG 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 41
EcoRII CCWGG 3 cut(s) 178, 192, 430
EcoT22I ATGCAT 1 cut(s) 480
FaeI CATG 2 cut(s) 264, 324
FatI CATG 2 cut(s) 260, 320
Fnu4HI GCNGC 3 cut(s) 150, 342, 507
FokI GGATG 3 cut(s) 86, 418, 447
Fsp4HI GCNGC 3 cut(s) 150, 342, 507
FspBI CTAG 4 cut(s) 114, 203, 255, 512
GluI GCNGC 3 cut(s) 150, 342, 507
GsuI CTGGAG 1 cut(s) 201
Hin1II CATG 2 cut(s) 264, 324
HincII GTYRAC 2 cut(s) 190, 222
HindII GTYRAC 2 cut(s) 190, 222
HindIII AAGCTT 1 cut(s) 105
HinfI GANTC 1 cut(s) 251
Hpy166II GTNNAC 3 cut(s) 190, 222, 548
Hpy188I TCNGA 2 cut(s) 121, 318
Hpy188III TCNNGA 4 cut(s) 9, 41, 255, 419
Hpy8I GTNNAC 3 cut(s) 190, 222, 548
HpyAV CCTTC 2 cut(s) 295, 560
HpyCH4III ACNGT 3 cut(s) 219, 283, 494
HpyCH4V TGCA 5 cut(s) 167, 173, 363, 478, 535
HpyF10VI GCNNNNNNNGC 1 cut(s) 512
HpyF3I CTNAG 1 cut(s) 332
Hsp92II CATG 2 cut(s) 264, 324
Kzo9I GATC 2 cut(s) 205, 393
Lsp1109I GCAGC 2 cut(s) 161, 493
LweI GCATC 3 cut(s) 81, 108, 465
MabI ACCWGGT 1 cut(s) 192
MaeI CTAG 4 cut(s) 114, 203, 255, 512
MaeIII GTNAC 3 cut(s) 127, 277, 559
MalI GATC 2 cut(s) 207, 395
MboI GATC 2 cut(s) 205, 393
MboII GAAGA 5 cut(s) 222, 280, 389, 392, 407
MluCI AATT 5 cut(s) 244, 271, 296, 411, 537
MnlI CCTC 5 cut(s) 37, 324, 327, 359, 396
Mph1103I ATGCAT 1 cut(s) 480
MroXI GAANNNNTTC 1 cut(s) 309
MseI TTAA 1 cut(s) 48
MslI CAYNNNNRTG 1 cut(s) 239
MspR9I CCNGG 3 cut(s) 180, 194, 432
MvaI CCWGG 3 cut(s) 180, 194, 432
MvnI CGCG 1 cut(s) 10
MwoI GCNNNNNNNGC 1 cut(s) 512
NdeII GATC 2 cut(s) 205, 393
NlaIII CATG 2 cut(s) 264, 324
NlaIV GGNNCC 1 cut(s) 429
NmuCI GTSAC 2 cut(s) 127, 559
NruI TCGCGA 1 cut(s) 10
NsiI ATGCAT 1 cut(s) 480
NspI RCATGY 1 cut(s) 264
PdmI GAANNNNTTC 1 cut(s) 309
PfeI GAWTC 1 cut(s) 251
PfoI TCCNGGA 1 cut(s) 430
PkrI GCNGC 3 cut(s) 151, 343, 508
PsiI TTATAA 1 cut(s) 542
Psp6I CCWGG 3 cut(s) 178, 192, 430
PspGI CCWGG 3 cut(s) 178, 192, 430
PspN4I GGNNCC 1 cut(s) 429
PstI CTGCAG 1 cut(s) 365
RruI TCGCGA 1 cut(s) 10
RsaI GTAC 2 cut(s) 198, 547
RsaNI GTAC 2 cut(s) 197, 546
RseI CAYNNNNRTG 1 cut(s) 239
SaqAI TTAA 1 cut(s) 48
SatI GCNGC 3 cut(s) 150, 342, 507
Sau3AI GATC 2 cut(s) 205, 393
ScrFI CCNGG 3 cut(s) 180, 194, 432
SetI ASST 7 cut(s) 48, 109, 162, 195, 204, 370, 508
SexAI ACCWGGT 1 cut(s) 192
SfaNI GCATC 3 cut(s) 81, 108, 465
SfcI CTRYAG 1 cut(s) 361
SmiMI CAYNNNNRTG 1 cut(s) 239
SpeI ACTAGT 1 cut(s) 113
Sse9I AATT 5 cut(s) 244, 271, 296, 411, 537
SsiI CCGC 1 cut(s) 342
SspMI CTAG 4 cut(s) 114, 203, 255, 512
StyD4I CCNGG 3 cut(s) 178, 192, 430
TaaI ACNGT 3 cut(s) 219, 283, 494
TasI AATT 5 cut(s) 244, 271, 296, 411, 537
TatI WGTACW 1 cut(s) 545
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 1 cut(s) 48
Tru9I TTAA 1 cut(s) 48
TseFI GTSAC 2 cut(s) 127, 559
TseI GCWGC 2 cut(s) 149, 506
Tsp45I GTSAC 2 cut(s) 127, 559
TspDTI ATGAA 3 cut(s) 17, 257, 422
XapI RAATTY 3 cut(s) 244, 271, 411
XbaI TCTAGA 1 cut(s) 254
XceI RCATGY 1 cut(s) 264
XmnI GAANNNNTTC 1 cut(s) 309
XspI CTAG 4 cut(s) 114, 203, 255, 512
Zsp2I ATGCAT 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.