RchiOBHm_Chr2g0141051

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002641
Physical Location & Seq
Reverse (-)
58625686 .. 58631015
5330 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51138

Sequence Viewer

Length: 1512 bp
ATGTACAGAACTGCAGCTTCGCGAGTCAGGTCGCTTAAGGGCCGTGTGGGCGCCACCAGGTATGCGTCAGTAACTGCAGCTGCTACAAAGCCAGCCTCAGGGGGTATCTTTAGCTGGCTGACTGGGGAAAAGTCCAGTGCTCTTCCTCGTCTGGAAACCCCATTAGCAGGTGTGAACCTCCCTCCTCCACTACCTGATTATGTGGAACCAAGCAAGACTAAGGTTACAAAGCTCTCCAATGGTGTCAGAATAGCTTCAGAAAAGTCACCGAGTCCTGCAGCCTCCATTGGATTATATGTTAATTGTGGATCCATTTATGAGACTCCAGTAACGAGTGGGGCTTCACACTTGTTAGAAAGGCTGGCCTTTAAGAGTACAACCAACCGGAGCCACTTGCGCATTATTAGAGAAGTGGAAGCAATTGGTGGTAACATTGGAGCCAGTGCCTCTAGGGAACAAATGGGATACACTTTTGATGCTCTTAAAACCTATGTTCCTCAGATGGTGGAATTGCTTGTTGACTCTGTGAGAAATCCTGCCTTCTTGGAATGGGAGGTCAATGAGGAGCTGGAAAAGGTGCGGGCAGAAATTGCTGACCTTTCTAAAAATCCTCAAGGCTTACTCTTGGAGAGGATTCATTCGGCTGGTTTTTCTGGAGCTTTGAGAAATCCTCTTTTGGCTTCTGAAGACGCACTGAACAGATTGGATAGCAACATCCTTGAGGAATTTGTTGCTGAGAATTATACTGCTCCTCGGATAGTTCTTGCAGCATCTGGGGTTGACCATGAGGAGCTTTTATCTATTGCAGAACCACTTCTCTCTGACCTACCAAGTGTGCCCCTTGTTGGCGAGCCAAAATCTCAATATGTTGGAGGGGAGTATCGTGCTCAAGGTGATACAAAGGATGAGGCACATGTTGCTCTTGCTTTTGAAGTTCCTGGTGGTTGGCGTGAAGAGAAGCAAGCTATCATTTTGACTGTTCTCCAGTTGCTTATGGGAGGAGGTGGCTCTTTCTCTGCCGGGGGTCCTGGAAAGGGGATGCACTCAAGGCTATATCTCCGTGTCTTGAATAATCATCAGGAGATTCAAAATTTTACTGCATTCAACAGCCTCTTTAATGATTCTGGATTGTTTGGAATTTATGCTAGCACTGGCTCTGGGTTTGCATCACAGGCAGTTGAAATAGCAGCAAAAGAGTTACTTTCAATTGCCACACCTGGACAAGTTTCGGATGTAGAGCTTAAACGTGCTAAAGAGTCCACAAAGGCTGCAGTTTTGATGAATCTAGAATCTAGAATGATTGCATCAGAAGATATAGGAAGGCAAGTTTTGACATATGGCGAGAGGAAACCCGTGGAGGATTTCTTAAAGACAGTAGAAGGAGTGACTTTGAAAGATATTACTACAGCCGCCCAACAGATTATATCTTCACCTCTAACAATGGCATCATATGGGAATGTTGTTAATGTCCCAAGCTACGATTCAGTTAACAGAATCTTCCATGCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000166 GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006508 GO:0006605 GO:0006626 GO:0006627 GO:0006807 GO:0006810 GO:0006839 GO:0006886 GO:0006950 GO:0006970 GO:0006996 GO:0007005 GO:0008104 GO:0008144 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009987 GO:0010033 GO:0010467 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016485 GO:0016787 GO:0017076 GO:0019538 GO:0019866 GO:0019867 GO:0030554 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0034982 GO:0035639 GO:0036094 GO:0042221 GO:0042886 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045184 GO:0045275 GO:0046872 GO:0046907 GO:0046914 GO:0050896 GO:0051179 GO:0051234 GO:0051604 GO:0051641 GO:0051649 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0070585 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072655 GO:0097159 GO:0097367 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901265 GO:1901363 GO:1901564 GO:1902494 GO:1990204
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C

Protein Analysis

503

Amino Acids

54.11

Weight (kDa)

5.89

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 83 - 227 1.7e-41 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 238 - 419 3.8e-31 Peptidase M16 inactive domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 158
Acc16I TGCGCA 1 cut(s) 398
Acc36I ACCTGC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 50
AccII CGCG 1 cut(s) 22
AciI CCGC 2 cut(s) 580, 1410
AclWI GGATC 2 cut(s) 303, 316
AcsI RAATTY 3 cut(s) 725, 1090, 1137
AcuI CTGAAG 2 cut(s) 240, 705
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 2 cut(s) 5, 376
AfiI CCNNNNNNNGG 4 cut(s) 98, 167, 845, 1034
AflII CTTAAG 1 cut(s) 35
AflIII ACRYGT 1 cut(s) 913
AgsI TTSAA 7 cut(s) 932, 1069, 1088, 1105, 1181, 1206, 1393
AjnI CCWGG 4 cut(s) 56, 937, 1027, 1216
AjuI GAANNNNNNNTTGG 2 cut(s) 1465, 1497
Alw21I GWGCWC 2 cut(s) 142, 889
Alw26I GTCTC 1 cut(s) 314
AlwI GGATC 2 cut(s) 303, 316
AlwNI CAGNNNCTG 2 cut(s) 74, 773
AoxI GGCC 2 cut(s) 40, 363
ApeKI GCWGC 7 cut(s) 14, 77, 80, 278, 767, 1187, 1268
ApoI RAATTY 3 cut(s) 725, 1090, 1137
Asp700I GAANNNNTTC 2 cut(s) 253, 813
AspLEI GCGC 2 cut(s) 53, 399
AspS9I GGNCC 2 cut(s) 40, 1025
AsuC2I CCSGG 1 cut(s) 1021
AsuHPI GGTGA 3 cut(s) 258, 905, 1422
AsuNHI GCTAGC 1 cut(s) 1145
AvaII GGWCC 1 cut(s) 1025
AxyI CCTNAGG 1 cut(s) 97
BaeGI GKGCMC 1 cut(s) 840
BamHI GGATCC 1 cut(s) 308
BanI GGYRCC 1 cut(s) 50
BbsI GAAGAC 1 cut(s) 693
Bbv12I GWGCWC 2 cut(s) 142, 889
BbvI GCAGC 7 cut(s) 26, 67, 89, 290, 779, 1199, 1255
BccI CCATC 1 cut(s) 496
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 4 cut(s) 58, 939, 1029, 1218
BciVI GTATCC 1 cut(s) 458
BcnI CCSGG 1 cut(s) 1021
BcoDI GTCTC 1 cut(s) 314
BfaI CTAG 4 cut(s) 450, 1146, 1286, 1293
BfmI CTRYAG 5 cut(s) 12, 75, 276, 1269, 1404
BfoI RGCGCY 1 cut(s) 54
BfrI CTTAAG 1 cut(s) 35
BfuAI ACCTGC 1 cut(s) 158
BfuI GTATCC 1 cut(s) 458
BglI GCCNNNNNGGC 1 cut(s) 48
BisI GCNGC 8 cut(s) 15, 78, 81, 279, 768, 1188, 1269, 1410
BlsI GCNGC 8 cut(s) 16, 79, 82, 280, 769, 1189, 1270, 1411
Bme1390I CCNGG 5 cut(s) 58, 939, 1021, 1029, 1218
Bme18I GGWCC 1 cut(s) 1025
BmgT120I GGNCC 2 cut(s) 40, 1025
BmiI GGNNCC 6 cut(s) 52, 207, 310, 389, 439, 1026
BmrFI CCNGG 5 cut(s) 58, 939, 1021, 1029, 1218
BmrI ACTGGG 1 cut(s) 132
BmsI GCATC 6 cut(s) 466, 779, 1029, 1175, 1313, 1454
BmtI GCTAGC 1 cut(s) 1149
BmuI ACTGGG 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 693
BplI GAGNNNNNCTC 2 cut(s) 655, 687
BpmI CTGGAG 3 cut(s) 309, 675, 968
BpuEI CTTGAG 4 cut(s) 597, 740, 873, 1030
BpuMI CCSGG 1 cut(s) 1021
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 3 cut(s) 752, 1020, 1353
BsaWI WCCGGW 1 cut(s) 384
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 167, 845, 1034
Bse1I ACTGG 6 cut(s) 127, 135, 326, 441, 985, 1156
Bse21I CCTNAGG 1 cut(s) 97
BseBI CCWGG 4 cut(s) 58, 939, 1029, 1218
BseDI CCNNGG 3 cut(s) 752, 1020, 1353
BseGI GGATG 4 cut(s) 714, 910, 1044, 1237
BseLI CCNNNNNNNGG 4 cut(s) 98, 167, 845, 1034
BseMII CTCAG 3 cut(s) 111, 512, 726
BseNI ACTGG 6 cut(s) 127, 135, 326, 441, 985, 1156
BseRI GAGGAG 5 cut(s) 174, 578, 741, 803, 1014
BseSI GKGCMC 1 cut(s) 840
BseXI GCAGC 7 cut(s) 26, 67, 89, 290, 779, 1199, 1255
Bsh1236I CGCG 1 cut(s) 22
BshFI GGCC 2 cut(s) 42, 365
BshNI GGYRCC 1 cut(s) 50
BsiHKAI GWGCWC 2 cut(s) 142, 889
BsiSI CCGG 2 cut(s) 385, 1020
BslFI GGGAC 1 cut(s) 1454
BslI CCNNNNNNNGG 4 cut(s) 98, 167, 845, 1034
BsmAI GTCTC 1 cut(s) 314
BsmFI GGGAC 1 cut(s) 1454
BsmI GAATGC 1 cut(s) 1100
BsnI GGCC 2 cut(s) 42, 365
Bsp1286I GDGCHC 3 cut(s) 142, 840, 889
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 1 cut(s) 308
Bsp68I TCGCGA 1 cut(s) 22
BspACI CCGC 2 cut(s) 580, 1410
BspANI GGCC 2 cut(s) 42, 365
BspCNI CTCAG 3 cut(s) 110, 511, 727
BspFNI CGCG 1 cut(s) 22
BspLI GGNNCC 6 cut(s) 52, 207, 310, 389, 439, 1026
BspMAI CTGCAG 4 cut(s) 16, 79, 280, 1273
BspMI ACCTGC 1 cut(s) 158
BspOI GCTAGC 1 cut(s) 1149
BspPI GGATC 2 cut(s) 303, 316
BspQI GCTCTTC 1 cut(s) 147
BspT107I GGYRCC 1 cut(s) 50
BspTI CTTAAG 1 cut(s) 35
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 6 cut(s) 127, 135, 326, 441, 985, 1156
BssECI CCNNGG 3 cut(s) 752, 1020, 1353
BssMI GATC 1 cut(s) 308
BssNI GRCGYC 1 cut(s) 51
Bst2UI CCWGG 4 cut(s) 58, 939, 1029, 1218
Bst4CI ACNGT 2 cut(s) 979, 1375
Bst6I CTCTTC 2 cut(s) 147, 948
BstACI GRCGYC 1 cut(s) 51
BstAFI CTTAAG 1 cut(s) 35
BstAPI GCANNNNNTGC 2 cut(s) 590, 917
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 7 cut(s) 93, 116, 363, 582, 851, 963, 1147
BstDEI CTNAG 4 cut(s) 97, 219, 498, 735
BstDSI CCRYGG 1 cut(s) 1353
BstF5I GGATG 4 cut(s) 714, 910, 1044, 1237
BstFNI CGCG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 2 cut(s) 53, 399
BstKTI GATC 1 cut(s) 311
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 1 cut(s) 308
BstMWI GCNNNNNNNGC 6 cut(s) 48, 396, 590, 917, 1048, 1172
BstNI CCWGG 4 cut(s) 58, 939, 1029, 1218
BstNSI RCATGY 1 cut(s) 917
BstSCI CCNGG 5 cut(s) 56, 937, 1019, 1027, 1216
BstSFI CTRYAG 5 cut(s) 12, 75, 276, 1269, 1404
BstSLI GKGCMC 1 cut(s) 840
BstUI CGCG 1 cut(s) 22
BstV1I GCAGC 7 cut(s) 26, 67, 89, 290, 779, 1199, 1255
BstV2I GAAGAC 1 cut(s) 693
BstX2I RGATCY 1 cut(s) 308
BstYI RGATCY 1 cut(s) 308
Bsu36I CCTNAGG 1 cut(s) 97
BsuI GTATCC 1 cut(s) 458
BsuRI GGCC 2 cut(s) 42, 365
BtgI CCRYGG 1 cut(s) 1353
BtsCI GGATG 4 cut(s) 714, 910, 1044, 1237
BtsIMutI CAGTG 4 cut(s) 142, 448, 692, 1149
BtuMI TCGCGA 1 cut(s) 22
BveI ACCTGC 1 cut(s) 158
Cac8I GCNNGC 7 cut(s) 93, 116, 363, 582, 851, 963, 1147
CaiI CAGNNNCTG 2 cut(s) 74, 773
CfoI GCGC 2 cut(s) 53, 399
Cfr13I GGNCC 2 cut(s) 40, 1025
CseI GACGC 2 cut(s) 54, 698
CsiI ACCWGGT 1 cut(s) 56
Csp6I GTAC 2 cut(s) 4, 375
CviAII CATG 3 cut(s) 785, 914, 1502
CviQI GTAC 2 cut(s) 4, 375
DdeI CTNAG 4 cut(s) 97, 219, 498, 735
DinI GGCGCC 1 cut(s) 52
DpnI GATC 1 cut(s) 310
DpnII GATC 1 cut(s) 308
Eam1104I CTCTTC 2 cut(s) 147, 948
EarI CTCTTC 2 cut(s) 147, 948
Eco47I GGWCC 1 cut(s) 1025
Eco57I CTGAAG 2 cut(s) 240, 705
Eco81I CCTNAGG 1 cut(s) 97
EcoO109I RGGNCCY 1 cut(s) 1025
EcoRII CCWGG 4 cut(s) 56, 937, 1027, 1216
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
FaeI CATG 3 cut(s) 788, 917, 1505
FalI AAGNNNNNCTT 2 cut(s) 1185, 1217
FaqI GGGAC 1 cut(s) 1454
FatI CATG 3 cut(s) 784, 913, 1501
FauI CCCGC 1 cut(s) 573
FauNDI CATATG 2 cut(s) 1336, 1450
Fnu4HI GCNGC 8 cut(s) 15, 78, 81, 279, 768, 1188, 1269, 1410
FokI GGATG 4 cut(s) 701, 917, 1051, 1244
Fsp4HI GCNGC 8 cut(s) 15, 78, 81, 279, 768, 1188, 1269, 1410
FspBI CTAG 4 cut(s) 450, 1146, 1286, 1293
FspI TGCGCA 1 cut(s) 398
GlaI GCGC 2 cut(s) 52, 398
GluI GCNGC 8 cut(s) 15, 78, 81, 279, 768, 1188, 1269, 1410
GsuI CTGGAG 3 cut(s) 309, 675, 968
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 2 cut(s) 42, 365
HapII CCGG 2 cut(s) 385, 1020
HgaI GACGC 2 cut(s) 54, 698
HhaI GCGC 2 cut(s) 53, 399
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 3 cut(s) 788, 917, 1505
Hin6I GCGC 2 cut(s) 51, 397
HinP1I GCGC 2 cut(s) 51, 397
HincII GTYRAC 3 cut(s) 520, 781, 1489
HindII GTYRAC 3 cut(s) 520, 781, 1489
HpaI GTTAAC 1 cut(s) 1489
HpaII CCGG 2 cut(s) 385, 1020
HphI GGTGA 3 cut(s) 258, 905, 1422
Hpy166II GTNNAC 5 cut(s) 175, 520, 781, 1260, 1489
Hpy188I TCNGA 8 cut(s) 248, 259, 501, 685, 756, 823, 1231, 1309
Hpy188III TCNNGA 8 cut(s) 21, 152, 654, 1066, 1079, 1125, 1286, 1293
Hpy8I GTNNAC 5 cut(s) 175, 520, 781, 1260, 1489
HpyAV CCTTC 3 cut(s) 550, 1314, 1373
HpyCH4III ACNGT 2 cut(s) 979, 1375
HpyCH4IV ACGT 1 cut(s) 1246
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 396, 590, 917, 1048, 1172
HpyF3I CTNAG 4 cut(s) 97, 219, 498, 735
HpySE526I ACGT 1 cut(s) 1246
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 3 cut(s) 788, 917, 1505
HspAI GCGC 2 cut(s) 51, 397
KasI GGCGCC 1 cut(s) 50
KspAI GTTAAC 1 cut(s) 1489
Kzo9I GATC 1 cut(s) 308
LguI GCTCTTC 1 cut(s) 147
LmnI GCTCC 6 cut(s) 387, 437, 565, 656, 754, 790
Lsp1109I GCAGC 7 cut(s) 26, 67, 89, 290, 779, 1199, 1255
LweI GCATC 6 cut(s) 466, 779, 1029, 1175, 1313, 1454
MabI ACCWGGT 1 cut(s) 56
MaeI CTAG 4 cut(s) 450, 1146, 1286, 1293
MaeII ACGT 1 cut(s) 1246
MaeIII GTNAC 7 cut(s) 70, 223, 264, 328, 428, 1197, 1384
MalI GATC 1 cut(s) 310
MboI GATC 1 cut(s) 308
MboII GAAGA 6 cut(s) 134, 698, 965, 1322, 1419, 1489
MfeI CAATTG 2 cut(s) 420, 1206
MflI RGATCY 1 cut(s) 308
MhlI GDGCHC 3 cut(s) 142, 840, 889
MluCI AATT 9 cut(s) 301, 420, 509, 588, 725, 739, 1090, 1137, 1206
Mly113I GGCGCC 1 cut(s) 51
MlyI GAGTC 5 cut(s) 33, 280, 316, 515, 1265
MmeI TCCRAC 1 cut(s) 850
MroXI GAANNNNTTC 2 cut(s) 253, 813
MseI TTAA 9 cut(s) 36, 300, 369, 483, 1116, 1242, 1367, 1464, 1488
MspA1I CMGCKG 1 cut(s) 80
MspCI CTTAAG 1 cut(s) 35
MspI CCGG 2 cut(s) 385, 1020
MspR9I CCNGG 5 cut(s) 58, 939, 1021, 1029, 1218
MunI CAATTG 2 cut(s) 420, 1206
Mva1269I GAATGC 1 cut(s) 1100
MvaI CCWGG 4 cut(s) 58, 939, 1029, 1218
MvnI CGCG 1 cut(s) 22
MwoI GCNNNNNNNGC 6 cut(s) 48, 396, 590, 917, 1048, 1172
NarI GGCGCC 1 cut(s) 51
NciI CCSGG 1 cut(s) 1021
NdeI CATATG 2 cut(s) 1336, 1450
NdeII GATC 1 cut(s) 308
NheI GCTAGC 1 cut(s) 1145
NlaIII CATG 3 cut(s) 788, 917, 1505
NlaIV GGNNCC 6 cut(s) 52, 207, 310, 389, 439, 1026
NmuCI GTSAC 2 cut(s) 264, 1384
NruI TCGCGA 1 cut(s) 22
NsbI TGCGCA 1 cut(s) 398
NspI RCATGY 1 cut(s) 917
PaqCI CACCTGC 1 cut(s) 158
PciI ACATGT 1 cut(s) 913
PciSI GCTCTTC 1 cut(s) 147
PctI GAATGC 1 cut(s) 1100
PdmI GAANNNNTTC 2 cut(s) 253, 813
PfeI GAWTC 7 cut(s) 634, 1084, 1121, 1282, 1289, 1481, 1494
PfoI TCCNGGA 1 cut(s) 1027
PkrI GCNGC 8 cut(s) 16, 79, 82, 280, 769, 1189, 1270, 1411
PleI GAGTC 5 cut(s) 32, 279, 316, 515, 1264
PluTI GGCGCC 1 cut(s) 54
PpsI GAGTC 5 cut(s) 32, 279, 316, 515, 1264
PpuMI RGGWCCY 1 cut(s) 1025
PscI ACATGT 1 cut(s) 913
Psp5II RGGWCCY 1 cut(s) 1025
Psp6I CCWGG 4 cut(s) 56, 937, 1027, 1216
PspGI CCWGG 4 cut(s) 56, 937, 1027, 1216
PspN4I GGNNCC 6 cut(s) 52, 207, 310, 389, 439, 1026
PspPI GGNCC 2 cut(s) 40, 1025
PspPPI RGGWCCY 1 cut(s) 1025
PstI CTGCAG 4 cut(s) 16, 79, 280, 1273
PstNI CAGNNNCTG 2 cut(s) 74, 773
PsuI RGATCY 1 cut(s) 308
PvuII CAGCTG 1 cut(s) 80
RruI TCGCGA 1 cut(s) 22
RsaI GTAC 2 cut(s) 5, 376
RsaNI GTAC 2 cut(s) 4, 375
SapI GCTCTTC 1 cut(s) 147
SaqAI TTAA 9 cut(s) 36, 300, 369, 483, 1116, 1242, 1367, 1464, 1488
SatI GCNGC 8 cut(s) 15, 78, 81, 279, 768, 1188, 1269, 1410
Sau3AI GATC 1 cut(s) 308
Sau96I GGNCC 2 cut(s) 40, 1025
SchI GAGTC 5 cut(s) 33, 280, 316, 515, 1265
ScrFI CCNGG 5 cut(s) 58, 939, 1021, 1029, 1218
SduI GDGCHC 3 cut(s) 142, 840, 889
SexAI ACCWGGT 1 cut(s) 56
SfaNI GCATC 6 cut(s) 466, 779, 1029, 1175, 1313, 1454
SfcI CTRYAG 5 cut(s) 12, 75, 276, 1269, 1404
SfoI GGCGCC 1 cut(s) 52
SinI GGWCC 1 cut(s) 1025
SmlI CTYRAG 5 cut(s) 35, 612, 719, 888, 1045
SmoI CTYRAG 5 cut(s) 35, 612, 719, 888, 1045
Sse9I AATT 9 cut(s) 301, 420, 509, 588, 725, 739, 1090, 1137, 1206
SsiI CCGC 2 cut(s) 580, 1410
SspDI GGCGCC 1 cut(s) 50
SspMI CTAG 4 cut(s) 450, 1146, 1286, 1293
StyD4I CCNGG 5 cut(s) 56, 937, 1019, 1027, 1216
TaaI ACNGT 2 cut(s) 979, 1375
TaiI ACGT 1 cut(s) 1249
TasI AATT 9 cut(s) 301, 420, 509, 588, 725, 739, 1090, 1137, 1206
TatI WGTACW 2 cut(s) 3, 374
TauI GCSGC 1 cut(s) 1412
TfiI GAWTC 7 cut(s) 634, 1084, 1121, 1282, 1289, 1481, 1494
Tru1I TTAA 9 cut(s) 36, 300, 369, 483, 1116, 1242, 1367, 1464, 1488
Tru9I TTAA 9 cut(s) 36, 300, 369, 483, 1116, 1242, 1367, 1464, 1488
TscAI CASTG 4 cut(s) 142, 448, 699, 1156
TseFI GTSAC 2 cut(s) 264, 1384
TseI GCWGC 7 cut(s) 14, 77, 80, 278, 767, 1187, 1268
Tsp45I GTSAC 2 cut(s) 264, 1384
TspDTI ATGAA 2 cut(s) 626, 1295
TspGWI ACGGA 1 cut(s) 1049
TspRI CASTG 4 cut(s) 142, 448, 699, 1156
Vha464I CTTAAG 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 1025
XapI RAATTY 3 cut(s) 725, 1090, 1137
XbaI TCTAGA 2 cut(s) 1285, 1292
XceI RCATGY 1 cut(s) 917
XmnI GAANNNNTTC 2 cut(s) 253, 813
XspI CTAG 4 cut(s) 450, 1146, 1286, 1293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.