FvH4_1g06620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002500
Physical Location & Seq
Reverse (-)
3478961 .. 3489120
10160 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06620.t1

Sequence Viewer

Length: 4323 bp
ATGGTTCCTTTTGCTTCATTTCTCCACCACCTTTTCACTATTCTCAGCTTCTTCACCCTCTTCACCATAAACCCTACTCTCTCCACTGATTTGGGCAATGAATTCTCAATCCTCAGCTTCAATTCTGATGACTTATATGGAGACTACTCGCCTCCATCGCCGCCGCCGAGTCCGCCGGAGCCTCGGCCGCCCTCACTCTCCTGCAGTGAGGGTCTGAATGCAACTGGGTCTTTCGACACCGTATGTGAGCTGAACTCTAGCTTGATTCTTGATAATGATGTGTATATACATGGAAATGGGAGCTTTTATATCCTCCCTGGTGTTAATTTGAGCTGCCCACATTTGGGGTGCTCCATTGTGGTTAAAATTAGTGGAGATTTTAGTTTAGGTAGCAATTCAGTGATTATTGCCGGGTTAGTTTCGGTTAATGCTTCCAATGCTAGCTTGTTTACTGGTTCAATGATCAATGTTACCGCGATGGCGGGGGAGCCGCCTCCAATGACTAGTGGCTCACCAAGGGGTGTGCAGGGTGCTGGTGGAGGTCATGGTGGGAGAGGAGCTAGTTGTGTCACAGATAACAGTAAGCTCCCAGATGATTTTTGGGGTGGGGATCCGTATGCTTGGTCATCGTTGGAAGAGCCGAAAAGTTTTGGGAGTAAGGGTGGGAGTACTAGTAAGGAGGTGAAGTATGGTGGGGAAGGAGGTGGGAGGATTTGGTTGGAGGCAAGAAACTCGATTGTTCTTGAGGGAAATGTTGTGGCTGATGGTGGTGATGGCGGTATGAAGGGTGGAGGAGGGTCAGGAGGCAGCATATACATTAAAGGTCTTAGAATGACTGGAAGTGGCAGGATAAGTGCAATTGGGGGTAACGGATTTGCTGGAGGAGGAGGGGGAAGAGTTTCTATCAATGTTTTTAGTAGGCGTGGTGAACCAGGAATCTTCAGTCATGGGGGAAGGAGTTTAGGCTGTCCTGAAAATGCAGGTGCTGCAGGGACATATTATGATGCTGTCCCTCGGAGGCTTATAGTCAGCAACCATAACTTGTCCACACGGACTGACACGCTTCTGTTGGAGTTTCCTAAACTGCCCCTCTGGACAAATGTTGATATACAAGATCATGCCAAGGCTTTGGTTCCTTTGTATTGGAGCCGTGTTCAGGTTAGAGGTCAAATTCGTTTATCATGTGCGGCAGTTTTGAGTTTTGGTCTTCAACAATTTGCTTCATCTGAGTTTGAGTTAATGGCAGAAGAGCTTCTCATGAGTGATTCTATCATCAAGATATTTGGAGCTCTTCGAATGTCTGTGAAAATGCACTTGATGTGGAATTCCAAAATGCTTATTGAGGGTGGTGTTGATACACTTGTGCAAACATCCTTGCTTGAAGCAACTAATTTAGTGGTTCTCAGGGAGTCCTCGGTGATACATTCTAATGCTAATTTGGGAGTTCATGGACAAGGTTTCTTGAATTTGTCTGGGGCAGGAGATGTTATTGAAGCGCAACACCTGGTTTTGTCTCTATTTTTTAGTATCAATGTTGGACCTGGATCTTTTCTGCGAGGGCCTTTGGATCACAGCAGTAGTAACTTTACGAGACCCCAACTCAACTGTGAGCTCCCAAATTGCCCTGCGGAATTACTTCATCCACCTGAAGATTGTAACATGAACTCTACATTGACCTTCACTCTTCAGATCTGTCGAGTTGAAGATGTTATTGTTAGAGGCATTATAACTGGATCTGTTATACATTTTCACTGGGTTAGAGAGGTAGTTGTACACTCTTCTGGGGTAATTACTGCATCTGGTCTGGGTTGCACTGGTGGGCTGGGTAGAGGAAGATTTTTTTTAAATGGTCTTGGCAGCGGTGGCGGGCATGGAGGCAAAGGTGGAGATGGGTTTTACGAGGGAAATTTTATTGAAGGCGGTGCTTCCTATGGAAATGCTGATTTACCTTGTGAACTTGGTAGTGGTAGTGGAAATGATAGTCTAGCTGGTGCAACAGCAGGTGGTGGTATTATTGTACTGGGTTCATTGGACCGCTCATTGTCTAGTTTGTCTGTTGCTGGCTCTCTTAGAGCTGATGGAGAAACCTTTGGAGAAAATTTTGGGGAGAATGTTGGTAAGACCATTTCAAATATAGGTCCTGGTGGTGGATCCGGTGGTACTATTCTTTTATTCGTTCAGACATTAGCACTTGGTGATTCTTCTACAATCTCAACTGTTGGTGGGCATGGTAGTCCTAGTGGTGGCGGTGGAGGTGGTGGTGGGAGGATTCACTTCCATTGGTCGGATATACCAGTTGGGGATGCATATTTACCCATTGCAAGCGTGGGAGGAAACATTAATACTGGGGGAGGATTCGGTAGAGGAAATGGTCGTGCTGGACAAAATGGGACTATCACTGGAAGGGCCTGTCCCAGAGGGTTATATGGTATTTTTTGTGAGGAATGCCCTCTTGGCACATTTAAGAATGTCAGTGGCTCTGCTAGATCCCTTTGTCGTGCTTGTCCGTCTTTTCAGCTACCGCATCGTGCCATATATGTTACTGTTCGAGGTGGTGTTACGGAGACTCCATGTCCATACAAGTGTATTTCTGATAGATATCACATGCCAAACTGTTATACTGCAGTTGAAGAATTGGTATACACTTTCGGTGGACCATGGTTGTTCAGTCTTATTCTGTTAGGTCTCCTCGTCTTCTTAGCTCTTGTGCTCAGTGTTGCACGATTGAAATATGTTACTACAGATGAATTGCCGGCTTCTGTACCTGCTCGACGGGGCTCCCGTTTAGATAATTCTTTTCCTTTCCTTGAATCGTTAAATGAGGTATTGGAAACAAATAGAAACGAGGAATCTCAGAGTCATGTCCATAGGATGTATTTCATGGGGCCAAATACATTCAGTGAACCTTGGCATCTACCTCATTCCCCTCCAGAACAAATAACAGATATAGTATATGAGGATGCATTCAATAGGTTTGTGGATGAGATCAATGGTTTAGCTGCTTATCAGTGGTGGGAAGGATCAGTCTACAGCATTCTTTCCATTCTTGCTTATCCACTTGCTTGGTCATGGCTACAGAGTCGACGGAAACATAAATTACAACAACTTCGTGAATTTGTTCGATCAGAATATGATCATTCTTGCTTGCGTTCTTGTCGCTCCCGTGCTCTATATGAAGGGATTAAGGTGGCTGCAACTACAGATCTGATGCTTGCATACGTGGACTTTTTCCTAGGTGGAGATGAGAAAAGAGCTGGCCTTCCTCCCCGTCTTCATCAGAGATTTCCAATGTCTATAATTTTTGGAGGAGATGGAAGTTACATGACTCCTTTCTCTCTTCAGAGTGATAACATTCTTACTAGCCTCATGAGTCAATCTGTTCCACCAACCATCTGGTATCGGCTTGTAGCCGGTCTAAATGCTCAATTGCGCTTAGTCCGGCATGGACACCTAAAATTGACTTTTAGTCACGTTATTAGCTGGCTTGAAACACATGTGAAACCCACTCTTGGTGCATTTGGCATACATGTTGATCTTGCCTGGTTTCAGCCTACAGCGTCTGGATATTGCCACTTTGGAATTGTGGTTTATACTACGGAAGTTGAAAGTCTGCCTCCTGAATTGGATGGTCAAGATGCATTCTTTCAACCAGAGCATCAGTCACGCATGCCAATAAATCATTGGGAAAACCAGATTGAACATATGAGACTCGTGGACCTTTGGATGTCTCAAAAGAAGTTTTCTGGAGGGATTTTACACACCAAAGGCTTGAGGATGTTAAAAGAGAAGAAAGCCATATGTTATCCCTTCGCTTTTGTACTTTATAATACTAAACCCATTGCTCATCAGGATCTCGTTGGTTTGGTCATTTCTGTTCTACTCTTGGGAGACTTTAGCTTAGTTTTGCTCACTTTGTTGCAGCACTATTCTATTTCACTCTTAAGTTTATTCTTAGTCTTGTTCATTCTTCCTCTCGGTCTACTGTTCCCCTTCCCGGCTGGAATCAGTGCTTTGTTTAGTCATGGGCCTAGACGTGCAGCAGGTCTTGCTCGTTTATATGCTTTGTGGAATATCACATCCCTGATCAATGTTGTTGTTGCTTTTATGTGTGGATTGATTCATTATATGACTCACTCAAATAGAAGGCATTCCAACTTTCCGTCCTGGAGTTTTAGCATGGATGAAAGTGAATGGTGGGTGCTTCCTTGTGGTCTAGCACTGTGTAAACTTGTCCAATCATGGCTCATTGATTGCCATGTTGCAAACCAGGAAATTCAGGATCACTCGTTGTACAGCAATGACCCTGAACTGTTCTGGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1441

Amino Acids

155.2

Weight (kDa)

6.15

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8003 PF26010 799 - 873 1.7e-34 Domain of unknown function (DUF8003)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1727, 3825
AarI CACCTGC 2 cut(s) 971, 1991
Acc36I ACCTGC 4 cut(s) 971, 1991, 2773, 4031
AccBSI CCGCTC 1 cut(s) 2037
AccI GTMKAC 4 cut(s) 2640, 3027, 3082, 3979
AccII CGCG 1 cut(s) 476
AcoI YGGCCR 1 cut(s) 185
AcsI RAATTY 8 cut(s) 101, 1170, 1324, 1465, 1906, 2098, 3113, 4272
AcuI CTGAAG 4 cut(s) 925, 1668, 1670, 3323
AdeI CACNNNGTG 1 cut(s) 2195
AfaI GTAC 7 cut(s) 670, 1773, 2019, 2161, 2763, 3819, 4292
AfiI CCNNNNNNNGG 8 cut(s) 343, 344, 1156, 2147, 2243, 2284, 3509, 3994
AflII CTTAAG 1 cut(s) 3940
AflIII ACRYGT 2 cut(s) 3493, 3526
AhdI GACNNNNNGTC 2 cut(s) 2571, 3465
AhlI ACTAGT 2 cut(s) 503, 671
AjiI CACGTC 1 cut(s) 4034
AjnI CCWGG 8 cut(s) 316, 931, 1503, 1540, 2140, 3539, 4163, 4266
AjuI GAANNNNNNNTTGG 2 cut(s) 2436, 2468
Alw21I GWGCWC 5 cut(s) 353, 1291, 1614, 2712, 3169
Alw26I GTCTC 8 cut(s) 135, 1518, 1585, 2558, 2690, 3700, 3732, 3882
AlwNI CAGNNNCTG 2 cut(s) 986, 3560
AoxI GGCC 6 cut(s) 185, 1559, 2406, 2885, 3256, 4025
ApeKI GCWGC 8 cut(s) 333, 807, 986, 1857, 2999, 3191, 3919, 4037
ApoI RAATTY 8 cut(s) 101, 1170, 1324, 1465, 1906, 2098, 3113, 4272
AseI ATTAAT 1 cut(s) 2340
Asp700I GAANNNNTTC 5 cut(s) 898, 1251, 1635, 3117, 4147
AspA2I CCTAGG 1 cut(s) 3232
AspLEI GCGC 2 cut(s) 1498, 3432
AspS9I GGNCC 9 cut(s) 1538, 1559, 2032, 2138, 2406, 2654, 2885, 3715, 4025
AsuC2I CCSGG 2 cut(s) 412, 3995
AsuHPI GGTGA 8 cut(s) 46, 55, 504, 694, 782, 938, 1429, 2207
AsuII TTCGAA 1 cut(s) 1294
AsuNHI GCTAGC 1 cut(s) 440
AvaII GGWCC 5 cut(s) 1538, 2032, 2138, 2654, 3715
AvrII CCTAGG 1 cut(s) 3232
BaeI ACNNNNGTAYC 2 cut(s) 1347, 1380
BamHI GGATCC 2 cut(s) 610, 2150
BanII GRGCYC 3 cut(s) 1291, 1614, 2780
BauI CACGAG 1 cut(s) 3710
BbsI GAAGAC 3 cut(s) 1199, 2686, 3263
Bbv12I GWGCWC 5 cut(s) 353, 1291, 1614, 2712, 3169
BbvCI CCTCAGC 1 cut(s) 113
BbvI GCAGC 8 cut(s) 320, 819, 973, 1869, 2986, 3178, 3931, 4049
BccI CCATC 9 cut(s) 163, 472, 758, 767, 1883, 2072, 3305, 3398, 3620
BceAI ACGGC 1 cut(s) 1134
BcgI CGANNNNNNTGC 2 cut(s) 714, 748
BciT130I CCWGG 8 cut(s) 318, 933, 1505, 1542, 2142, 3541, 4165, 4268
BclI TGATCA 3 cut(s) 462, 3133, 4083
BcnI CCSGG 2 cut(s) 412, 3995
BcoDI GTCTC 8 cut(s) 135, 1518, 1585, 2558, 2690, 3700, 3732, 3882
BcuI ACTAGT 2 cut(s) 503, 671
BfmI CTRYAG 8 cut(s) 202, 987, 2622, 2739, 3028, 3074, 3198, 3552
BfrI CTTAAG 1 cut(s) 3940
BfuAI ACCTGC 4 cut(s) 971, 1991, 2773, 4031
BglI GCCNNNNNGGC 1 cut(s) 2454
BglII AGATCT 2 cut(s) 1689, 3202
BlnI CCTAGG 1 cut(s) 3232
BmcAI AGTACT 1 cut(s) 670
Bme18I GGWCC 5 cut(s) 1538, 2032, 2138, 2654, 3715
BmeRI GACNNNNNGTC 2 cut(s) 2571, 3465
BmgBI CACGTC 1 cut(s) 4034
BmgT120I GGNCC 9 cut(s) 1538, 1559, 2032, 2138, 2406, 2654, 2885, 3715, 4025
BmiI GGNNCC 9 cut(s) 6, 180, 489, 612, 1134, 1148, 2152, 2779, 2886
BmrI ACTGGG 4 cut(s) 234, 1762, 2030, 2355
BmsI GCATC 9 cut(s) 994, 1805, 2293, 2533, 2920, 2950, 3198, 3625, 3664
BmtI GCTAGC 1 cut(s) 444
BmuI ACTGGG 4 cut(s) 234, 1762, 2030, 2355
BpiI GAAGAC 3 cut(s) 1199, 2686, 3263
BplI GAGNNNNNCTC 2 cut(s) 239, 271
BpmI CTGGAG 4 cut(s) 900, 2913, 3765, 4186
Bpu10I CCTNAGC 1 cut(s) 113
Bpu14I TTCGAA 1 cut(s) 1294
BpuEI CTTGAG 2 cut(s) 764, 3790
BpuMI CCSGG 2 cut(s) 412, 3995
BsaAI YACGTR 1 cut(s) 3220
BsaBI GATNNNNATC 1 cut(s) 2598
BsaI GGTCTC 2 cut(s) 1585, 2690
BsaJI CCNNGG 9 cut(s) 182, 316, 515, 1013, 1122, 1413, 2657, 2906, 3232
BsaWI WCCGGW 1 cut(s) 2153
BsaXI ACNNNNNCTCC 8 cut(s) 646, 658, 671, 676, 688, 701, 2551, 2581
Bsc4I CCNNNNNNNGG 8 cut(s) 343, 344, 1156, 2147, 2243, 2284, 3509, 3994
Bse118I RCCGGY 2 cut(s) 2752, 3410
Bse3DI GCAATG 4 cut(s) 103, 2316, 3837, 4303
Bse8I GATNNNNATC 1 cut(s) 2598
BseBI CCWGG 8 cut(s) 318, 933, 1505, 1542, 2142, 3541, 4165, 4268
BseDI CCNNGG 9 cut(s) 182, 316, 515, 1013, 1122, 1413, 2657, 2906, 3232
BseJI GATNNNNATC 1 cut(s) 2598
BseLI CCNNNNNNNGG 8 cut(s) 343, 344, 1156, 2147, 2243, 2284, 3509, 3994
BseMI GCAATG 4 cut(s) 103, 2316, 3837, 4303
BseMII CTCAG 6 cut(s) 58, 127, 1218, 1417, 2725, 2867
BseRI GAGGAG 6 cut(s) 570, 807, 897, 900, 2678, 3321
BseX3I CGGCCG 1 cut(s) 185
BseXI GCAGC 8 cut(s) 320, 819, 973, 1869, 2986, 3178, 3931, 4049
BseYI CCCAGC 1 cut(s) 1822
BsgI GTGCAG 2 cut(s) 545, 4056
Bsh1236I CGCG 1 cut(s) 476
Bsh1285I CGRYCG 1 cut(s) 188
BshFI GGCC 6 cut(s) 187, 1561, 2408, 2887, 3258, 4027
BsiEI CGRYCG 1 cut(s) 188
BsiHKAI GWGCWC 5 cut(s) 353, 1291, 1614, 2712, 3169
BsiSI CCGG 7 cut(s) 176, 411, 2154, 2753, 3411, 3439, 3995
BslFI GGGAC 4 cut(s) 995, 1006, 2397, 2404
BslI CCNNNNNNNGG 8 cut(s) 343, 344, 1156, 2147, 2243, 2284, 3509, 3994
BsmAI GTCTC 8 cut(s) 135, 1518, 1585, 2558, 2690, 3700, 3732, 3882
BsmFI GGGAC 4 cut(s) 995, 1006, 2397, 2404
BsmI GAATGC 6 cut(s) 223, 2450, 2963, 3033, 3638, 4147
BsnI GGCC 6 cut(s) 187, 1561, 2408, 2887, 3258, 4027
Bso31I GGTCTC 2 cut(s) 1585, 2690
Bsp119I TTCGAA 1 cut(s) 1294
Bsp1286I GDGCHC 6 cut(s) 353, 1291, 1614, 2712, 2780, 3169
Bsp1407I TGTACA 2 cut(s) 1771, 4290
Bsp19I CCATGG 1 cut(s) 2657
BspANI GGCC 6 cut(s) 187, 1561, 2408, 2887, 3258, 4027
BspCNI CTCAG 6 cut(s) 57, 126, 1219, 1416, 2724, 2866
BspFNI CGCG 1 cut(s) 476
BspHI TCATGA 2 cut(s) 1257, 3366
BspLI GGNNCC 9 cut(s) 6, 180, 489, 612, 1134, 1148, 2152, 2779, 2886
BspMAI CTGCAG 3 cut(s) 206, 991, 2626
BspMI ACCTGC 4 cut(s) 971, 1991, 2773, 4031
BspOI GCTAGC 1 cut(s) 444
BspQI GCTCTTC 3 cut(s) 630, 1242, 1296
BspT104I TTCGAA 1 cut(s) 1294
BspTI CTTAAG 1 cut(s) 3940
BspTNI GGTCTC 2 cut(s) 1585, 2690
BsrBI CCGCTC 1 cut(s) 2037
BsrDI GCAATG 4 cut(s) 103, 2316, 3837, 4303
BsrFI RCCGGY 2 cut(s) 2752, 3410
BsrGI TGTACA 2 cut(s) 1771, 4290
BssAI RCCGGY 2 cut(s) 2752, 3410
BssECI CCNNGG 9 cut(s) 182, 316, 515, 1013, 1122, 1413, 2657, 2906, 3232
BssNAI GTATAC 1 cut(s) 2641
BssSI CACGAG 1 cut(s) 3710
BssT1I CCWWGG 5 cut(s) 515, 1122, 2657, 2906, 3232
Bst1107I GTATAC 1 cut(s) 2641
Bst2BI CACGAG 1 cut(s) 3710
Bst2UI CCWGG 8 cut(s) 318, 933, 1505, 1542, 2142, 3541, 4165, 4268
Bst4CI ACNGT 9 cut(s) 241, 581, 1607, 2218, 2545, 2615, 3984, 4221, 4311
Bst6I CTCTTC 8 cut(s) 65, 630, 889, 1242, 1296, 1689, 1783, 3342
BstAFI CTTAAG 1 cut(s) 3940
BstAPI GCANNNNNTGC 2 cut(s) 986, 4046
BstAUI TGTACA 2 cut(s) 1771, 4290
BstBAI YACGTR 1 cut(s) 3220
BstBI TTCGAA 1 cut(s) 1294
BstDSI CCRYGG 1 cut(s) 2657
BstFNI CGCG 1 cut(s) 476
BstHHI GCGC 2 cut(s) 1498, 3432
BstMAI GTCTC 8 cut(s) 135, 1518, 1585, 2558, 2690, 3700, 3732, 3882
BstMCI CGRYCG 1 cut(s) 188
BstMWI GCNNNNNNNGC 8 cut(s) 157, 172, 187, 437, 986, 1863, 2454, 4046
BstNI CCWGG 8 cut(s) 318, 933, 1505, 1542, 2142, 3541, 4165, 4268
BstNSI RCATGY 4 cut(s) 2608, 3497, 3530, 3670
BstSFI CTRYAG 8 cut(s) 202, 987, 2622, 2739, 3028, 3074, 3198, 3552
BstUI CGCG 1 cut(s) 476
BstV1I GCAGC 8 cut(s) 320, 819, 973, 1869, 2986, 3178, 3931, 4049
BstV2I GAAGAC 3 cut(s) 1199, 2686, 3263
BstX2I RGATCY 8 cut(s) 610, 1544, 1689, 1733, 2150, 2486, 3202, 3850
BstXI CCANNNNNNTGG 4 cut(s) 91, 1129, 3063, 3393
BstYI RGATCY 8 cut(s) 610, 1544, 1689, 1733, 2150, 2486, 3202, 3850
BstZ17I GTATAC 1 cut(s) 2641
BstZI CGGCCG 1 cut(s) 185
BsuRI GGCC 6 cut(s) 187, 1561, 2408, 2887, 3258, 4027
BtgI CCRYGG 1 cut(s) 2657
BtgZI GCGATG 2 cut(s) 141, 491
BtrI CACGTC 1 cut(s) 4034
BtsI GCAGTG 1 cut(s) 211
BveI ACCTGC 4 cut(s) 971, 1991, 2773, 4031
CaiI CAGNNNCTG 2 cut(s) 986, 3560
CciI TCATGA 2 cut(s) 1257, 3366
CfoI GCGC 2 cut(s) 1498, 3432
Cfr10I RCCGGY 2 cut(s) 2752, 3410
Cfr13I GGNCC 9 cut(s) 1538, 1559, 2032, 2138, 2406, 2654, 2885, 3715, 4025
CseI GACGC 1 cut(s) 3546
CsiI ACCWGGT 1 cut(s) 1503
Csp6I GTAC 7 cut(s) 669, 1772, 2018, 2160, 2762, 3818, 4291
CviQI GTAC 7 cut(s) 669, 1772, 2018, 2160, 2762, 3818, 4291
DraI TTTAAA 1 cut(s) 1845
DraIII CACNNNGTG 1 cut(s) 2195
DriI GACNNNNNGTC 2 cut(s) 2571, 3465
EaeI YGGCCR 1 cut(s) 185
EagI CGGCCG 1 cut(s) 185
Eam1104I CTCTTC 8 cut(s) 65, 630, 889, 1242, 1296, 1689, 1783, 3342
Eam1105I GACNNNNNGTC 2 cut(s) 2571, 3465
EarI CTCTTC 8 cut(s) 65, 630, 889, 1242, 1296, 1689, 1783, 3342
EciI GGCGGA 1 cut(s) 162
Ecl136II GAGCTC 2 cut(s) 1289, 1612
EclXI CGGCCG 1 cut(s) 185
Eco130I CCWWGG 5 cut(s) 515, 1122, 2657, 2906, 3232
Eco24I GRGCYC 3 cut(s) 1291, 1614, 2780
Eco31I GGTCTC 2 cut(s) 1585, 2690
Eco32I GATATC 1 cut(s) 2600
Eco47I GGWCC 5 cut(s) 1538, 2032, 2138, 2654, 3715
Eco52I CGGCCG 1 cut(s) 185
Eco53kI GAGCTC 2 cut(s) 1289, 1612
Eco57I CTGAAG 4 cut(s) 925, 1668, 1670, 3323
EcoICRI GAGCTC 2 cut(s) 1289, 1612
EcoO109I RGGNCCY 3 cut(s) 1559, 2138, 2406
EcoRI GAATTC 2 cut(s) 101, 1324
EcoRII CCWGG 8 cut(s) 316, 931, 1503, 1540, 2140, 3539, 4163, 4266
EcoRV GATATC 1 cut(s) 2600
EcoT14I CCWWGG 5 cut(s) 515, 1122, 2657, 2906, 3232
EcoT22I ATGCAT 3 cut(s) 2308, 2965, 3640
EcoT38I GRGCYC 3 cut(s) 1291, 1614, 2780
ErhI CCWWGG 5 cut(s) 515, 1122, 2657, 2906, 3232
FaqI GGGAC 4 cut(s) 995, 1006, 2397, 2404
FauI CCCGC 2 cut(s) 475, 1859
FauNDI CATATG 2 cut(s) 3702, 3797
FbaI TGATCA 3 cut(s) 462, 3133, 4083
FblI GTMKAC 4 cut(s) 2640, 3027, 3082, 3979
FriOI GRGCYC 3 cut(s) 1291, 1614, 2780
GlaI GCGC 2 cut(s) 1497, 3431
GsaI CCCAGC 1 cut(s) 1826
GsuI CTGGAG 4 cut(s) 900, 2913, 3765, 4186
HaeIII GGCC 6 cut(s) 187, 1561, 2408, 2887, 3258, 4027
HapII CCGG 7 cut(s) 176, 411, 2154, 2753, 3411, 3439, 3995
HgaI GACGC 1 cut(s) 3546
HhaI GCGC 2 cut(s) 1498, 3432
Hin6I GCGC 2 cut(s) 1496, 3430
HinP1I GCGC 2 cut(s) 1496, 3430
HincII GTYRAC 1 cut(s) 3083
HindII GTYRAC 1 cut(s) 3083
HpaII CCGG 7 cut(s) 176, 411, 2154, 2753, 3411, 3439, 3995
HphI GGTGA 8 cut(s) 46, 55, 504, 694, 782, 938, 1429, 2207
Hpy99I CGWCG 2 cut(s) 2775, 3087
HpyCH4III ACNGT 9 cut(s) 241, 581, 1607, 2218, 2545, 2615, 3984, 4221, 4311
HpyCH4IV ACGT 3 cut(s) 3219, 3471, 4033
HpyF10VI GCNNNNNNNGC 8 cut(s) 157, 172, 187, 437, 986, 1863, 2454, 4046
HpySE526I ACGT 3 cut(s) 3219, 3471, 4033
HspAI GCGC 2 cut(s) 1496, 3430
KroI GCCGGC 1 cut(s) 2752
KroNI GCCGGC 1 cut(s) 2754
Ksp22I TGATCA 3 cut(s) 462, 3133, 4083
LguI GCTCTTC 3 cut(s) 630, 1242, 1296
Lsp1109I GCAGC 8 cut(s) 320, 819, 973, 1869, 2986, 3178, 3931, 4049
LweI GCATC 9 cut(s) 994, 1805, 2293, 2533, 2920, 2950, 3198, 3625, 3664
MabI ACCWGGT 1 cut(s) 1503
MaeII ACGT 3 cut(s) 3219, 3471, 4033
MbiI CCGCTC 1 cut(s) 2037
MfeI CAATTG 2 cut(s) 858, 3425
MflI RGATCY 8 cut(s) 610, 1544, 1689, 1733, 2150, 2486, 3202, 3850
MhlI GDGCHC 6 cut(s) 353, 1291, 1614, 2712, 2780, 3169
MlyI GAGTC 9 cut(s) 178, 1418, 2560, 2866, 3088, 3319, 3379, 3702, 4123
MmeI TCCRAC 6 cut(s) 612, 699, 1050, 1516, 2265, 4176
Mph1103I ATGCAT 3 cut(s) 2308, 2965, 3640
MroNI GCCGGC 1 cut(s) 2752
MroXI GAANNNNTTC 5 cut(s) 898, 1251, 1635, 3117, 4147
MslI CAYNNNNRTG 3 cut(s) 294, 1428, 2581
MspA1I CMGCKG 1 cut(s) 1860
MspCI CTTAAG 1 cut(s) 3940
MspI CCGG 7 cut(s) 176, 411, 2154, 2753, 3411, 3439, 3995
MunI CAATTG 2 cut(s) 858, 3425
Mva1269I GAATGC 6 cut(s) 223, 2450, 2963, 3033, 3638, 4147
MvaI CCWGG 8 cut(s) 318, 933, 1505, 1542, 2142, 3541, 4165, 4268
MvnI CGCG 1 cut(s) 476
MwoI GCNNNNNNNGC 8 cut(s) 157, 172, 187, 437, 986, 1863, 2454, 4046
NaeI GCCGGC 1 cut(s) 2754
NciI CCSGG 2 cut(s) 412, 3995
NcoI CCATGG 1 cut(s) 2657
NdeI CATATG 2 cut(s) 3702, 3797
NgoMIV GCCGGC 1 cut(s) 2752
NheI GCTAGC 1 cut(s) 440
NlaIV GGNNCC 9 cut(s) 6, 180, 489, 612, 1134, 1148, 2152, 2779, 2886
NmeAIII GCCGAG 2 cut(s) 163, 192
NmuCI GTSAC 3 cut(s) 568, 3467, 3660
NsiI ATGCAT 3 cut(s) 2308, 2965, 3640
NspI RCATGY 4 cut(s) 2608, 3497, 3530, 3670
NspV TTCGAA 1 cut(s) 1294
PaeI GCATGC 1 cut(s) 3670
PagI TCATGA 2 cut(s) 1257, 3366
PaqCI CACCTGC 2 cut(s) 971, 1991
PciI ACATGT 2 cut(s) 3493, 3526
PciSI GCTCTTC 3 cut(s) 630, 1242, 1296
PcsI WCGNNNNNNNCGW 2 cut(s) 164, 2779
PctI GAATGC 6 cut(s) 223, 2450, 2963, 3033, 3638, 4147
PdiI GCCGGC 1 cut(s) 2754
PdmI GAANNNNTTC 5 cut(s) 898, 1251, 1635, 3117, 4147
PfoI TCCNGGA 1 cut(s) 4163
PleI GAGTC 9 cut(s) 177, 1417, 2560, 2865, 3087, 3319, 3378, 3702, 4123
PpsI GAGTC 9 cut(s) 177, 1417, 2560, 2865, 3087, 3319, 3378, 3702, 4123
Ppu21I YACGTR 1 cut(s) 3220
PpuMI RGGWCCY 1 cut(s) 2138
PscI ACATGT 2 cut(s) 3493, 3526
PshBI ATTAAT 1 cut(s) 2340
PsiI TTATAA 2 cut(s) 1727, 3825
Psp124BI GAGCTC 2 cut(s) 1291, 1614
Psp5II RGGWCCY 1 cut(s) 2138
Psp6I CCWGG 8 cut(s) 316, 931, 1503, 1540, 2140, 3539, 4163, 4266
PspFI CCCAGC 1 cut(s) 1822
PspGI CCWGG 8 cut(s) 316, 931, 1503, 1540, 2140, 3539, 4163, 4266
PspN4I GGNNCC 9 cut(s) 6, 180, 489, 612, 1134, 1148, 2152, 2779, 2886
PspPI GGNCC 9 cut(s) 1538, 1559, 2032, 2138, 2406, 2654, 2885, 3715, 4025
PspPPI RGGWCCY 1 cut(s) 2138
PstI CTGCAG 3 cut(s) 206, 991, 2626
PstNI CAGNNNCTG 2 cut(s) 986, 3560
PsuI RGATCY 8 cut(s) 610, 1544, 1689, 1733, 2150, 2486, 3202, 3850
RsaI GTAC 7 cut(s) 670, 1773, 2019, 2161, 2763, 3819, 4292
RsaNI GTAC 7 cut(s) 669, 1772, 2018, 2160, 2762, 3818, 4291
RseI CAYNNNNRTG 3 cut(s) 294, 1428, 2581
SacI GAGCTC 2 cut(s) 1291, 1614
SalI GTCGAC 1 cut(s) 3081
SapI GCTCTTC 3 cut(s) 630, 1242, 1296
Sau96I GGNCC 9 cut(s) 1538, 1559, 2032, 2138, 2406, 2654, 2885, 3715, 4025
ScaI AGTACT 1 cut(s) 670
SchI GAGTC 9 cut(s) 178, 1418, 2560, 2866, 3088, 3319, 3379, 3702, 4123
SduI GDGCHC 6 cut(s) 353, 1291, 1614, 2712, 2780, 3169
SexAI ACCWGGT 1 cut(s) 1503
SfaNI GCATC 9 cut(s) 994, 1805, 2293, 2533, 2920, 2950, 3198, 3625, 3664
SfcI CTRYAG 8 cut(s) 202, 987, 2622, 2739, 3028, 3074, 3198, 3552
SfuI TTCGAA 1 cut(s) 1294
SinI GGWCC 5 cut(s) 1538, 2032, 2138, 2654, 3715
SmiMI CAYNNNNRTG 3 cut(s) 294, 1428, 2581
SmlI CTYRAG 3 cut(s) 743, 3769, 3940
SmoI CTYRAG 3 cut(s) 743, 3769, 3940
SpeI ACTAGT 2 cut(s) 503, 671
SphI GCATGC 1 cut(s) 3670
SstI GAGCTC 2 cut(s) 1291, 1614
StyI CCWWGG 5 cut(s) 515, 1122, 2657, 2906, 3232
TaaI ACNGT 9 cut(s) 241, 581, 1607, 2218, 2545, 2615, 3984, 4221, 4311
TaiI ACGT 3 cut(s) 3222, 3474, 4036
TaqI TCGA 8 cut(s) 234, 734, 1294, 1696, 2548, 2770, 3082, 3121
TaqII GACCGA 1 cut(s) 3965
TatI WGTACW 5 cut(s) 668, 1771, 2017, 3817, 4290
TauI GCSGC 5 cut(s) 163, 166, 190, 493, 1190
TseFI GTSAC 3 cut(s) 568, 3467, 3660
TseI GCWGC 8 cut(s) 333, 807, 986, 1857, 2999, 3191, 3919, 4037
Tsp45I GTSAC 3 cut(s) 568, 3467, 3660
TspGWI ACGGA 8 cut(s) 603, 885, 1066, 2496, 2576, 3100, 3611, 4149
Vha464I CTTAAG 1 cut(s) 3940
VpaK11BI GGWCC 5 cut(s) 1538, 2032, 2138, 2654, 3715
VspI ATTAAT 1 cut(s) 2340
XapI RAATTY 8 cut(s) 101, 1170, 1324, 1465, 1906, 2098, 3113, 4272
XceI RCATGY 4 cut(s) 2608, 3497, 3530, 3670
XcmI CCANNNNNNNNNTGG 4 cut(s) 597, 2323, 3390, 3678
XmaJI CCTAGG 1 cut(s) 3232
XmiI GTMKAC 4 cut(s) 2640, 3027, 3082, 3979
XmnI GAANNNNTTC 5 cut(s) 898, 1251, 1635, 3117, 4147
ZrmI AGTACT 1 cut(s) 670
Zsp2I ATGCAT 3 cut(s) 2308, 2965, 3640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.