Rh2AG071100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002500
Physical Location & Seq
Reverse (-)
5856928 .. 5863369
6442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG071100.1

Sequence Viewer

Length: 2667 bp
ATGAGTGGTTGGCTTGGATTGCTGCTCCAGATTTGTCGAGTTGAATATGTTATTATTGAAGGCATTATAACTGGATCTGTTATACATTTTCACTGGGTGAGAGATGTAGTTGTACACTCTTCTGGGGTAATTACTGCATCTGGTCTGGGTTGCACTGGTGGGCTGGGTAGAGGAAGATTTTTTGTAAATGGTCTTGGCAGCGGTGGCGGGCATGGAGGCAAAGGTGGAGATGGGTTTTACGACGGAAATTTTATTGAAGGTGGTGTTTCCTATGGAAATGCTGATTTACCTTGTGAACTTGGTAGTGGTAGTGGAAATGATAGTCTAGCTGGTGCAACAGCAGGTGGTGGTATTATTGTAATGGGTTCATTGGAGCGCTCATTGTCTAGTTTCACTCTTGCGGGCTCTCTTAGAGCTGATGGAGAAACCTTTGGAGAAAATTTTGGGGAGAAAGTTGGTCAGACCATTTCAAATATAGGTCCTGGTGGTGGATCTGGTGGAACCATTCTTTTGTTTGTTCAGACATTAGCACTTGGTGATTCTTCTACAATCTCAACTGTTGGTGGGCATGGTAGTCCTAGTGGTGGTGGTGGAGGAGGTGGTGGGAGGATTCACTTCCATTGGTCGGACATACCAGTTGGGGATGCATATCTACCCATAGCAAGCGTGCGAGGAAACATTAATACTGGGGGAGGATTCGGTAGAGGAAATGGGCGTGCTGGACAAAATGGGACTATCACTGGAAGGGCCTGTCCCAGAGGGTTATATGGTATCTTTTGTGAGGAATGCCCTCTTGGCACCTTTAAGAATGTCAGTGGCTCTGCTAGAGCCCTTTGTCATGCTTGTCCTTCGTTTCAGCTTCCGCATCGTGCCATATATGTTACCATTCGAGGTGGTGTTACTGAGACTCCATGTCCATACAAGTGCGTTTCTGACAGATATCACATGCCAAACTGTTATACAGCAGTTGAAGAATTGGTATACACTTTCGGTGGACCGTGGTTGTTCTGTCTTATTCTGTTAGGTCTTCTCGTCCTCTTAGCTCTTGTGCTCAGTGTTGCACGAATGAAATATGTTACTGCAGACGAATTACCGGCTGCTGTACCTGCTCGACGGGGCTCCCGTATAGATCATTCTTTCCCTTTCCTTGAATCGTTAAATGAGGTTTTGGAAACAAATAGAAATGAGGAATCTCAGAGTCATGTGCATAGGATGTATTTCACGGGGCCAAATACATTCAGTGAACCTTGGCATCTACCTCATTCCCCTCCAGAACAAATAACAGAGATAGTATATGAGGATGCATTCAATCGGTTTGTGGATGAGATTAATGGTTTAGCTGCTTATCAGTGGTGGGAAGGATCAGTCTACAGCATTCTTTCTATTCTTGCCTATCCACTTGCTTGGTGTTGGCTACAGAGTCGACGGAAACAGAAATTGCAACAACTTCGTGAATTTGTTCGATCAGAATATGATCATTCTTGCTTGCGTTCTTGTCGTTCCCGTGCACTATATGAAGGGATTAAGGTGGCTGCAACTACGGATTTGATGCTTGCATATGTGGACTTTTTCCTAGGTGGAGATGAGAAAAGAGCTGGCCTTCCTCCCCGTCTTCATCAGAGATTTCCAATGTCTATAATTTTTGGAGGAGATGGAAGTTACATGGCTCCTTTCTCTCTTCAGAGTGATAACATTCTTACTAGCCTCATGAGTCAGTCTGTTCCACCTACCATCTGGTATCGGCTTGTAGCCGGTCTAAATGCTCAGCTGCGCTTAGTCCGGCATGGACACTTAAAACTGACTTTTAGTCATGTTACTAGCTGGCTTGAAACACATGCAAAACCTACTCTTGGTGCATTTGGCATACATGTTGATCTTGCCTGGTTTCAGCCTACAGCGTCTGGATATTGCCACTTCGGAATCGTGGTTTATGCTATGGAAGTTGAAAGTCTGCCATCTGAATTGGACGGTCAAGGTGCATTCTTTCTACCAGAGCATCAGTCACGCATGCCAAGAAATCATTGGGAAAACCAAATTGAACATTTGAGACTCATCGACCTTTGGATATCTCAAAAGAAGTTTTGTGGAGGGATTTTACACACCAAAGGCTTGAGGACGTTAAAAGAGAAGAAGGCCATATGTTATCCCTTCTCTTTTATACTTTATAATGCTAAACCCATTGGCCATCAGGATCTTGTTGGTTTGGTCATTTCTATTTTACTCTTGGGAGACTTTAGCTTAGTTTTGCTCACTTTGCTCCAGCTGTATTCTATTTCACTCTTAAGTTTATTCTTAGTCTTGTTCATTCTTCCTCTCGGTCTACTATTCCCCTTCCCGGCTGGAATCAGCGCTTTGTTTAGTCACGGGGCTAGACGTTCAGCAGGTCTTGCCCGTTTATATGCTTTGTGGAATCTCACATCCTTGATCAATGTTGTAGTTGCTTTTATATGTGGATTGATTCATTATACGACTCACTCAAATAAAAAGCATTCCAATTTTCAGTCCTGGACTTTTAGCATGGATGAAAGTGAATGGTGGGTGCTTCCTTGTGGTCTAGTACTGTGCAAAGTTGTCCAATCACGACTTATTGATTGCCTCGTGGCAAACCAGGAGATTCAGGATCACTCACTGTACAGCAATGACCCTGAACTGTTCTGGCAGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

888

Amino Acids

96.98

Weight (kDa)

6.71

Isoelectric Point (pI)

49.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8003 PF26010 246 - 320 1.6e-34 Domain of unknown function (DUF8003)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 68, 2166
AarI CACCTGC 1 cut(s) 332
Acc36I ACCTGC 3 cut(s) 332, 1114, 2372
AccB1I GGYRCC 1 cut(s) 797
AccI GTMKAC 4 cut(s) 981, 1368, 1423, 2320
AciI CCGC 4 cut(s) 201, 207, 401, 863
AclWI GGATC 5 cut(s) 82, 499, 1369, 2199, 2628
AcoI YGGCCR 1 cut(s) 2182
AcsI RAATTY 3 cut(s) 247, 439, 1454
AcuI CTGAAG 1 cut(s) 1664
AdeI CACNNNGTG 2 cut(s) 97, 536
AfaI GTAC 4 cut(s) 114, 1104, 2559, 2633
AfeI AGCGCT 2 cut(s) 377, 2350
AfiI CCNNNNNNNGG 5 cut(s) 488, 584, 625, 1850, 2335
AflII CTTAAG 1 cut(s) 2281
AflIII ACRYGT 1 cut(s) 1867
AhdI GACNNNNNGTC 2 cut(s) 912, 1806
AjnI CCWGG 4 cut(s) 481, 1880, 2504, 2607
AjuI GAANNNNNNNTTGG 2 cut(s) 777, 809
Alw21I GWGCWC 2 cut(s) 1053, 1510
Alw26I GTCTC 3 cut(s) 899, 2041, 2223
Alw44I GTGCAC 1 cut(s) 1506
AlwI GGATC 5 cut(s) 82, 499, 1369, 2199, 2628
AlwNI CAGNNNCTG 1 cut(s) 1901
Aor51HI AGCGCT 2 cut(s) 377, 2350
AoxI GGCC 5 cut(s) 747, 1226, 1597, 2133, 2182
ApaLI GTGCAC 1 cut(s) 1506
ApeKI GCWGC 6 cut(s) 22, 198, 1097, 1340, 1532, 1768
ApoI RAATTY 3 cut(s) 247, 439, 1454
AseI ATTAAT 2 cut(s) 681, 1329
Asp700I GAANNNNTTC 1 cut(s) 1458
AspA2I CCTAGG 1 cut(s) 1573
AspLEI GCGC 3 cut(s) 378, 1773, 2351
AspS9I GGNCC 4 cut(s) 479, 747, 995, 1226
AsuC2I CCSGG 1 cut(s) 2336
AsuHPI GGTGA 2 cut(s) 109, 548
AvaII GGWCC 2 cut(s) 479, 995
AvrII CCTAGG 1 cut(s) 1573
BaeGI GKGCMC 1 cut(s) 1510
BalI TGGCCA 1 cut(s) 2184
BanI GGYRCC 1 cut(s) 797
BanII GRGCYC 3 cut(s) 407, 832, 1121
BauI CACGAG 1 cut(s) 2597
BbsI GAAGAC 2 cut(s) 1019, 1604
Bbv12I GWGCWC 2 cut(s) 1053, 1510
BbvI GCAGC 6 cut(s) 9, 210, 1084, 1327, 1519, 1755
BccI CCATC 6 cut(s) 224, 413, 1646, 1739, 1963, 2193
BcgI CGANNNNNNTGC 2 cut(s) 1430, 1464
BciT130I CCWGG 4 cut(s) 483, 1882, 2506, 2609
BclI TGATCA 2 cut(s) 1474, 2424
BcnI CCSGG 1 cut(s) 2336
BcoDI GTCTC 3 cut(s) 899, 2041, 2223
BfaI CTAG 9 cut(s) 326, 387, 579, 825, 1574, 1701, 1818, 2370, 2555
BfmI CTRYAG 4 cut(s) 1080, 1369, 1415, 1893
BfoI RGCGCY 2 cut(s) 379, 2352
BfrI CTTAAG 1 cut(s) 2281
BfuAI ACCTGC 3 cut(s) 332, 1114, 2372
BglI GCCNNNNNGGC 1 cut(s) 795
BisI GCNGC 6 cut(s) 23, 199, 1098, 1341, 1533, 1769
BlnI CCTAGG 1 cut(s) 1573
BlpI GCTNAGC 1 cut(s) 1764
BlsI GCNGC 6 cut(s) 24, 200, 1099, 1342, 1534, 1770
BmcAI AGTACT 1 cut(s) 2559
Bme1390I CCNGG 5 cut(s) 483, 1882, 2336, 2506, 2609
Bme18I GGWCC 2 cut(s) 479, 995
BmeRI GACNNNNNGTC 2 cut(s) 912, 1806
BmgT120I GGNCC 4 cut(s) 479, 747, 995, 1226
BmiI GGNNCC 5 cut(s) 502, 799, 1120, 1227, 1668
BmrFI CCNGG 5 cut(s) 483, 1882, 2336, 2506, 2609
BmrI ACTGGG 2 cut(s) 103, 696
BmsI GCATC 7 cut(s) 146, 634, 874, 1261, 1291, 1539, 2005
BmuI ACTGGG 2 cut(s) 103, 696
BpiI GAAGAC 2 cut(s) 1019, 1604
BpmI CTGGAG 3 cut(s) 11, 1254, 2243
Bpu1102I GCTNAGC 1 cut(s) 1764
BpuEI CTTGAG 1 cut(s) 2131
BpuMI CCSGG 1 cut(s) 2336
BsaBI GATNNNNATC 1 cut(s) 648
BsaJI CCNNGG 3 cut(s) 998, 1247, 1573
BsaXI ACNNNNNCTCC 2 cut(s) 892, 922
Bsc4I CCNNNNNNNGG 5 cut(s) 488, 584, 625, 1850, 2335
Bse118I RCCGGY 2 cut(s) 1093, 1751
Bse1I ACTGG 6 cut(s) 76, 98, 160, 635, 691, 745
Bse3DI GCAATG 1 cut(s) 2644
Bse8I GATNNNNATC 1 cut(s) 648
BseBI CCWGG 4 cut(s) 483, 1882, 2506, 2609
BseDI CCNNGG 3 cut(s) 998, 1247, 1573
BseGI GGATG 6 cut(s) 649, 1218, 1306, 1327, 2417, 2527
BseJI GATNNNNATC 1 cut(s) 648
BseLI CCNNNNNNNGG 5 cut(s) 488, 584, 625, 1850, 2335
BseMI GCAATG 1 cut(s) 2644
BseMII CTCAG 4 cut(s) 894, 1066, 1208, 1778
BseNI ACTGG 6 cut(s) 76, 98, 160, 635, 691, 745
BseRI GAGGAG 2 cut(s) 609, 1662
BseSI GKGCMC 1 cut(s) 1510
BseXI GCAGC 6 cut(s) 9, 210, 1084, 1327, 1519, 1755
BseYI CCCAGC 1 cut(s) 163
BshFI GGCC 5 cut(s) 749, 1228, 1599, 2135, 2184
BshNI GGYRCC 1 cut(s) 797
BsiHKAI GWGCWC 2 cut(s) 1053, 1510
BsiSI CCGG 4 cut(s) 1094, 1752, 1780, 2336
BslFI GGGAC 2 cut(s) 738, 745
BslI CCNNNNNNNGG 5 cut(s) 488, 584, 625, 1850, 2335
BsmAI GTCTC 3 cut(s) 899, 2041, 2223
BsmFI GGGAC 2 cut(s) 738, 745
BsmI GAATGC 5 cut(s) 791, 1304, 1374, 1979, 2488
BsnI GGCC 5 cut(s) 749, 1228, 1599, 2135, 2184
Bsp1286I GDGCHC 5 cut(s) 407, 832, 1053, 1121, 1510
Bsp1407I TGTACA 2 cut(s) 112, 2631
Bsp1720I GCTNAGC 1 cut(s) 1764
BspACI CCGC 4 cut(s) 201, 207, 401, 863
BspANI GGCC 5 cut(s) 749, 1228, 1599, 2135, 2184
BspCNI CTCAG 4 cut(s) 895, 1065, 1207, 1777
BspHI TCATGA 1 cut(s) 1707
BspLI GGNNCC 5 cut(s) 502, 799, 1120, 1227, 1668
BspMAI CTGCAG 1 cut(s) 1084
BspMI ACCTGC 3 cut(s) 332, 1114, 2372
BspPI GGATC 5 cut(s) 82, 499, 1369, 2199, 2628
BspT107I GGYRCC 1 cut(s) 797
BspTI CTTAAG 1 cut(s) 2281
BsrDI GCAATG 1 cut(s) 2644
BsrFI RCCGGY 2 cut(s) 1093, 1751
BsrGI TGTACA 2 cut(s) 112, 2631
BsrI ACTGG 6 cut(s) 76, 98, 160, 635, 691, 745
BssAI RCCGGY 2 cut(s) 1093, 1751
BssECI CCNNGG 3 cut(s) 998, 1247, 1573
BssNAI GTATAC 1 cut(s) 982
BssSI CACGAG 1 cut(s) 2597
BssT1I CCWWGG 2 cut(s) 1247, 1573
Bst1107I GTATAC 1 cut(s) 982
Bst2BI CACGAG 1 cut(s) 2597
Bst2UI CCWGG 4 cut(s) 483, 1882, 2506, 2609
Bst4CI ACNGT 7 cut(s) 559, 956, 999, 1970, 2562, 2631, 2652
Bst6I CTCTTC 2 cut(s) 124, 1683
BstAFI CTTAAG 1 cut(s) 2281
BstAPI GCANNNNNTGC 1 cut(s) 2387
BstAUI TGTACA 2 cut(s) 112, 2631
BstDEI CTNAG 9 cut(s) 410, 903, 1039, 1052, 1194, 1764, 1774, 2239, 2293
BstDSI CCRYGG 1 cut(s) 998
BstF5I GGATG 6 cut(s) 649, 1218, 1306, 1327, 2417, 2527
BstH2I RGCGCY 2 cut(s) 379, 2352
BstHHI GCGC 3 cut(s) 378, 1773, 2351
BstMAI GTCTC 3 cut(s) 899, 2041, 2223
BstMWI GCNNNNNNNGC 6 cut(s) 19, 204, 795, 1106, 2254, 2387
BstNI CCWGG 4 cut(s) 483, 1882, 2506, 2609
BstNSI RCATGY 4 cut(s) 949, 1838, 1871, 2011
BstSCI CCNGG 5 cut(s) 481, 1880, 2334, 2504, 2607
BstSFI CTRYAG 4 cut(s) 1080, 1369, 1415, 1893
BstSLI GKGCMC 1 cut(s) 1510
BstV1I GCAGC 6 cut(s) 9, 210, 1084, 1327, 1519, 1755
BstV2I GAAGAC 2 cut(s) 1019, 1604
BstX2I RGATCY 3 cut(s) 74, 491, 2191
BstXI CCANNNNNNTGG 1 cut(s) 1404
BstYI RGATCY 3 cut(s) 74, 491, 2191
BstZ17I GTATAC 1 cut(s) 982
BsuRI GGCC 5 cut(s) 749, 1228, 1599, 2135, 2184
BtgI CCRYGG 1 cut(s) 998
BtsCI GGATG 6 cut(s) 649, 1218, 1306, 1327, 2417, 2527
BtsIMutI CAGTG 8 cut(s) 91, 153, 738, 820, 1060, 1246, 1355, 2627
BveI ACCTGC 3 cut(s) 332, 1114, 2372
CaiI CAGNNNCTG 1 cut(s) 1901
CciI TCATGA 1 cut(s) 1707
CfoI GCGC 3 cut(s) 378, 1773, 2351
Cfr10I RCCGGY 2 cut(s) 1093, 1751
Cfr13I GGNCC 4 cut(s) 479, 747, 995, 1226
CseI GACGC 1 cut(s) 1887
Csp6I GTAC 4 cut(s) 113, 1103, 2558, 2632
CviQI GTAC 4 cut(s) 113, 1103, 2558, 2632
DdeI CTNAG 9 cut(s) 410, 903, 1039, 1052, 1194, 1764, 1774, 2239, 2293
DraIII CACNNNGTG 2 cut(s) 97, 536
DriI GACNNNNNGTC 2 cut(s) 912, 1806
EaeI YGGCCR 1 cut(s) 2182
Eam1104I CTCTTC 2 cut(s) 124, 1683
Eam1105I GACNNNNNGTC 2 cut(s) 912, 1806
EarI CTCTTC 2 cut(s) 124, 1683
Eco130I CCWWGG 2 cut(s) 1247, 1573
Eco24I GRGCYC 3 cut(s) 407, 832, 1121
Eco32I GATATC 2 cut(s) 941, 2067
Eco47I GGWCC 2 cut(s) 479, 995
Eco47III AGCGCT 2 cut(s) 377, 2350
Eco57I CTGAAG 1 cut(s) 1664
EcoO109I RGGNCCY 2 cut(s) 479, 747
EcoRII CCWGG 4 cut(s) 481, 1880, 2504, 2607
EcoRV GATATC 2 cut(s) 941, 2067
EcoT14I CCWWGG 2 cut(s) 1247, 1573
EcoT22I ATGCAT 2 cut(s) 649, 1306
EcoT38I GRGCYC 3 cut(s) 407, 832, 1121
ErhI CCWWGG 2 cut(s) 1247, 1573
FaqI GGGAC 2 cut(s) 738, 745
FauI CCCGC 2 cut(s) 200, 394
FauNDI CATATG 2 cut(s) 1558, 2138
FbaI TGATCA 2 cut(s) 1474, 2424
FblI GTMKAC 4 cut(s) 981, 1368, 1423, 2320
Fnu4HI GCNGC 6 cut(s) 23, 199, 1098, 1341, 1533, 1769
FokI GGATG 6 cut(s) 656, 1225, 1313, 1334, 2404, 2534
FriOI GRGCYC 3 cut(s) 407, 832, 1121
Fsp4HI GCNGC 6 cut(s) 23, 199, 1098, 1341, 1533, 1769
FspBI CTAG 9 cut(s) 326, 387, 579, 825, 1574, 1701, 1818, 2370, 2555
GlaI GCGC 3 cut(s) 377, 1772, 2350
GluI GCNGC 6 cut(s) 23, 199, 1098, 1341, 1533, 1769
GsaI CCCAGC 1 cut(s) 167
GsuI CTGGAG 3 cut(s) 11, 1254, 2243
HaeII RGCGCY 2 cut(s) 379, 2352
HaeIII GGCC 5 cut(s) 749, 1228, 1599, 2135, 2184
HapII CCGG 4 cut(s) 1094, 1752, 1780, 2336
HgaI GACGC 1 cut(s) 1887
HhaI GCGC 3 cut(s) 378, 1773, 2351
Hin6I GCGC 3 cut(s) 376, 1771, 2349
HinP1I GCGC 3 cut(s) 376, 1771, 2349
HincII GTYRAC 1 cut(s) 1424
HindII GTYRAC 1 cut(s) 1424
HpaII CCGG 4 cut(s) 1094, 1752, 1780, 2336
HphI GGTGA 2 cut(s) 109, 548
Hpy188III TCNNGA 9 cut(s) 28, 1271, 1451, 1708, 1902, 2189, 2580, 2618, 2664
Hpy99I CGWCG 3 cut(s) 245, 1116, 1428
HpyCH4III ACNGT 7 cut(s) 559, 956, 999, 1970, 2562, 2631, 2652
HpyCH4IV ACGT 2 cut(s) 2117, 2374
HpyF10VI GCNNNNNNNGC 6 cut(s) 19, 204, 795, 1106, 2254, 2387
HpyF3I CTNAG 9 cut(s) 410, 903, 1039, 1052, 1194, 1764, 1774, 2239, 2293
HpySE526I ACGT 2 cut(s) 2117, 2374
HspAI GCGC 3 cut(s) 376, 1771, 2349
Ksp22I TGATCA 2 cut(s) 1474, 2424
LmnI GCTCC 5 cut(s) 30, 373, 1124, 1672, 2262
Lsp1109I GCAGC 6 cut(s) 9, 210, 1084, 1327, 1519, 1755
LweI GCATC 7 cut(s) 146, 634, 874, 1261, 1291, 1539, 2005
MaeI CTAG 9 cut(s) 326, 387, 579, 825, 1574, 1701, 1818, 2370, 2555
MaeII ACGT 2 cut(s) 2117, 2374
MaeIII GTNAC 7 cut(s) 880, 898, 1075, 1658, 1813, 2001, 2360
MboII GAAGA 9 cut(s) 111, 186, 534, 983, 1019, 1604, 1670, 2140, 2300
MflI RGATCY 3 cut(s) 74, 491, 2191
MhlI GDGCHC 5 cut(s) 407, 832, 1053, 1121, 1510
MlsI TGGCCA 1 cut(s) 2184
MluNI TGGCCA 1 cut(s) 2184
MlyI GAGTC 6 cut(s) 901, 1207, 1429, 1720, 2043, 2464
MmeI TCCRAC 1 cut(s) 606
Mox20I TGGCCA 1 cut(s) 2184
Mph1103I ATGCAT 2 cut(s) 649, 1306
MroXI GAANNNNTTC 1 cut(s) 1458
MscI TGGCCA 1 cut(s) 2184
MseI TTAA 8 cut(s) 681, 804, 1157, 1329, 1524, 1793, 2120, 2282
MslI CAYNNNNRTG 1 cut(s) 922
Msp20I TGGCCA 1 cut(s) 2184
MspA1I CMGCKG 3 cut(s) 201, 1768, 2263
MspCI CTTAAG 1 cut(s) 2281
MspI CCGG 4 cut(s) 1094, 1752, 1780, 2336
MspR9I CCNGG 5 cut(s) 483, 1882, 2336, 2506, 2609
Mva1269I GAATGC 5 cut(s) 791, 1304, 1374, 1979, 2488
MvaI CCWGG 4 cut(s) 483, 1882, 2506, 2609
MwoI GCNNNNNNNGC 6 cut(s) 19, 204, 795, 1106, 2254, 2387
NciI CCSGG 1 cut(s) 2336
NdeI CATATG 2 cut(s) 1558, 2138
NlaIV GGNNCC 5 cut(s) 502, 799, 1120, 1227, 1668
NmuCI GTSAC 2 cut(s) 2001, 2360
NsiI ATGCAT 2 cut(s) 649, 1306
NspI RCATGY 4 cut(s) 949, 1838, 1871, 2011
PaeI GCATGC 1 cut(s) 2011
PagI TCATGA 1 cut(s) 1707
PaqCI CACCTGC 1 cut(s) 332
PciI ACATGT 1 cut(s) 1867
PcsI WCGNNNNNNNCGW 1 cut(s) 1120
PctI GAATGC 5 cut(s) 791, 1304, 1374, 1979, 2488
PdmI GAANNNNTTC 1 cut(s) 1458
PfoI TCCNGGA 1 cut(s) 2504
PkrI GCNGC 6 cut(s) 24, 200, 1099, 1342, 1534, 1770
PleI GAGTC 6 cut(s) 901, 1206, 1428, 1719, 2043, 2464
PpsI GAGTC 6 cut(s) 901, 1206, 1428, 1719, 2043, 2464
PpuMI RGGWCCY 1 cut(s) 479
PscI ACATGT 1 cut(s) 1867
PshBI ATTAAT 2 cut(s) 681, 1329
PsiI TTATAA 2 cut(s) 68, 2166
Psp5II RGGWCCY 1 cut(s) 479
Psp6I CCWGG 4 cut(s) 481, 1880, 2504, 2607
PspFI CCCAGC 1 cut(s) 163
PspGI CCWGG 4 cut(s) 481, 1880, 2504, 2607
PspN4I GGNNCC 5 cut(s) 502, 799, 1120, 1227, 1668
PspPI GGNCC 4 cut(s) 479, 747, 995, 1226
PspPPI RGGWCCY 1 cut(s) 479
PstI CTGCAG 1 cut(s) 1084
PstNI CAGNNNCTG 1 cut(s) 1901
PsuI RGATCY 3 cut(s) 74, 491, 2191
PvuII CAGCTG 2 cut(s) 1768, 2263
RsaI GTAC 4 cut(s) 114, 1104, 2559, 2633
RsaNI GTAC 4 cut(s) 113, 1103, 2558, 2632
RseI CAYNNNNRTG 1 cut(s) 922
SalI GTCGAC 1 cut(s) 1422
SaqAI TTAA 8 cut(s) 681, 804, 1157, 1329, 1524, 1793, 2120, 2282
SatI GCNGC 6 cut(s) 23, 199, 1098, 1341, 1533, 1769
Sau96I GGNCC 4 cut(s) 479, 747, 995, 1226
ScaI AGTACT 1 cut(s) 2559
SchI GAGTC 6 cut(s) 901, 1207, 1429, 1720, 2043, 2464
ScrFI CCNGG 5 cut(s) 483, 1882, 2336, 2506, 2609
SduI GDGCHC 5 cut(s) 407, 832, 1053, 1121, 1510
SfaNI GCATC 7 cut(s) 146, 634, 874, 1261, 1291, 1539, 2005
SfcI CTRYAG 4 cut(s) 1080, 1369, 1415, 1893
SinI GGWCC 2 cut(s) 479, 995
SmiMI CAYNNNNRTG 1 cut(s) 922
SmlI CTYRAG 2 cut(s) 2110, 2281
SmoI CTYRAG 2 cut(s) 2110, 2281
SphI GCATGC 1 cut(s) 2011
SsiI CCGC 4 cut(s) 201, 207, 401, 863
SspMI CTAG 9 cut(s) 326, 387, 579, 825, 1574, 1701, 1818, 2370, 2555
StyD4I CCNGG 5 cut(s) 481, 1880, 2334, 2504, 2607
StyI CCWWGG 2 cut(s) 1247, 1573
TaaI ACNGT 7 cut(s) 559, 956, 999, 1970, 2562, 2631, 2652
TaiI ACGT 2 cut(s) 2120, 2377
TaqI TCGA 6 cut(s) 37, 889, 1111, 1423, 1462, 2055
TaqII GACCGA 1 cut(s) 2306
TatI WGTACW 3 cut(s) 112, 2557, 2631
Tru1I TTAA 8 cut(s) 681, 804, 1157, 1329, 1524, 1793, 2120, 2282
Tru9I TTAA 8 cut(s) 681, 804, 1157, 1329, 1524, 1793, 2120, 2282
TscAI CASTG 8 cut(s) 98, 160, 745, 820, 1060, 1246, 1355, 2634
TseFI GTSAC 2 cut(s) 2001, 2360
TseI GCWGC 6 cut(s) 22, 198, 1097, 1340, 1532, 1768
Tsp45I GTSAC 2 cut(s) 2001, 2360
TspDTI ATGAA 7 cut(s) 357, 1082, 1530, 1604, 2293, 2450, 2538
TspGWI ACGGA 3 cut(s) 258, 1441, 1556
TspRI CASTG 8 cut(s) 98, 160, 745, 820, 1060, 1246, 1355, 2634
Vha464I CTTAAG 1 cut(s) 2281
VneI GTGCAC 1 cut(s) 1506
VpaK11BI GGWCC 2 cut(s) 479, 995
VspI ATTAAT 2 cut(s) 681, 1329
XapI RAATTY 3 cut(s) 247, 439, 1454
XceI RCATGY 4 cut(s) 949, 1838, 1871, 2011
XcmI CCANNNNNNNNNTGG 2 cut(s) 1731, 2019
XmaJI CCTAGG 1 cut(s) 1573
XmiI GTMKAC 4 cut(s) 981, 1368, 1423, 2320
XmnI GAANNNNTTC 1 cut(s) 1458
XspI CTAG 9 cut(s) 326, 387, 579, 825, 1574, 1701, 1818, 2370, 2555
ZrmI AGTACT 1 cut(s) 2559
Zsp2I ATGCAT 2 cut(s) 649, 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.