MD02G1069200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002500
Physical Location & Seq
Reverse (-)
5602774 .. 5610699
7926 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1069200.v1.1.491

Sequence Viewer

Length: 4314 bp
ATGGCTCAAAGCGCCCCGTTTCTTCTCCACCTTTTCATCCTCAACCTCTTCATCAGCTTCGCCATAAACCCTAGCCTCTGCGCTGATTCCGACGATGAATTTTCAATTCTCGCGTTCGAAATTCACGGGGACTACTCGCCTCCTTCTCCTCCGCCGGTTCCGCCGCCGCCCCACCCTCCCCCGCTCTCGTGCCAGGGGGGTCTCAATGGAATTGGGTCTCTGGACACCATTTGTGAGCTGAATTCAAGCTTGATTCTTGAAGATAATGTGTATATAGAAGGAAATGGGAGCTTATTTATACTTTCCGGTGTTAATTTGAGCTGCCCATTTTTAGGTTGCGAAATTGTGGTTAACATTAGTGGGGAATTCAGTTTAGGTAGAAATTCAATGCTTATTGCCGGGTTAGTTACGGTTAATGCTTCGAATGCGAGCTTTTTAACTGGTTCAGTGGTAAATGTTACGGCATTGGCGGGTGCACCTCCTGAGCAGACTAGTGGCACCCCCGACGGTCTTCAGGGTTCTGGTGGAGGGCATGGTGGGAGAGGTGCTAGTTGTGTGATTGATAATACGAAGCTCCCCGACGATGTTTGGGGCGGAGATACGTATGCTTGGTCATCGTTAAATGAGCCGGTGAGTTATGGGAGTAAGGGTGGAACAACTAGTAAGGATGAGAAGTATGGTGGGGAAGGAGGTGGGAGGATTTGGTTGGAGGCAAGAAGTTCAATTGAACTTAGTGGGAGTGTTTTGGCTGATGGCGGTGATGGTGGGATTAAGGGTGGTGGAGGGTCAGGGGGTAGCATATTTGTCAAAGCTCGTCGAATGACTGGAAGTGGCAGGATAAGTGCAGTGGGGGGTAATGGATTTGCAGGAGGAGGAGGTGGAAGAGTTTCCATCAAAGTTTTCAGCAGGCGTGATAATACAGATATCGTTGCTCATGGGGGAAGGAGTTTAGGCTGTCCTGAAAATGCAGGTGCTGCAGGGACATATTATGATGCTGTTCCTCGGAGACTTATAGTCAATAATCATAACTTGTCCACACAGACTGACACGCTTCTGTTAGAGTTTCCCAAACAGCCACTTTGGACAAATGTTGATATCCAAAATCATGCCAAGGCTTTGGTTCCTTTGTATTGGAGCCGTGTTCAGGTTCGAGGTCAAATTCGTTTATCATGTGGTGCAGTTTTGAGTTTTGGTCTTGCACATTTTGCTTCATCAGAATTTGAGTTAATGGCAGAAGAGCTTCTGATGAGTGATTCTGTTATCAAGATATTTGGGGCTCTTCGAATGTCTGTCAAAATGCACTTGATGTGGAATTCTAAAATGCTTATAGATGGTCTTGCTGATGCAATTGTGGCAACATCCTTGCTTGAGGCAAGCAATGTAGTGGTTCTCAGGGGGGCGTCCGTGATACATTCTAATGCAAATTTGGGAGTTCATGGACAAGGTTTCTTGAATTTGTCTGGGCCAGGAGATCTGATTGAAGCACAACACCTGATTCTGTCTTTATTTTTCAGTGTCAATATTGGACCTGGATCTTCACTGCGAGGGCCTCTGGAAAGTGGTGGTAGTAATTTGACGAAACCCAAACTCAACTGTGAACTTCCAAACTGCCCCATGGAATTACTTCATCCACCCGAAGATTGTAACATGAACTCTACATTGACCTTCACTCTTCAGATCTGTCGAGTTGAAGATGTTATTATTCAAGGCAGTATAACTGGATCTGTTATACATTTTCACTGGGTTAGAGCTGTAGAGGTCCACTCTTCTGGGGTAATTAGTGCATCTGGCCTAGGCTGCCCTGGTGGGATTGGTAGAGGAAAGTTTTTCGCAAATGGGCTTGGTGGCGGTGGTGGGCATGGTGGCAAAGGTGGGGATGGGTATTACGATGGAAATTTTATTGATGGTGGTGTTTCCTATGGAGATGCTGATTTGCCGTGTGAACTTGGTAGTGGTAGTGGAAATGATAGCCTAGCTGGTGCAACTGCAGGTGGTGGTATCATTGTAATGGGTTCATTGGAGCGCTCATTGTCAAGTTTGTCTCTTGATGGTTCACTTAGAGCCGACGGAGAATCCTTTGGAGAAAAATATCTGGAGAAAAATGATAGGGTCTTTTCAAATATAGGTCCTGGGGGTGGTTCTGGTGGAACTATTCTATTGTTTGTTCAAACATTGGCACTTGGTAATTCTACTACAATCTCAACTGTTGGTGGACATGGTAGTCCTAGTGGTGGTGGTGGAGGAGGTGGTGGGAGGATTCACTTTCACTGGTCAGATATACCAGTTGGGGATGCATATCTACCCATAGCAAGTGCGAATGGAAGCATCATTACTGGGGGAGGCTTCGGTAGAGGTCATGGTCGGGCTGGACAAAATGGGACTGTTACCGGAAAGGCCTGTCCCAGAGGGCTTTATGGTATCTTTTGTGAGGAATGCCCTGTTGGCAGCTTTAAGAATGTGAGTGGCTCTGATAGAGCCCTTTGTCATGCTTGCCCATCTTTGGAGCTTCCACATCGTGCCATATATGTCACGGTTCGAGGTGGTGTCACTGAAACTCCATGTCCGCATAAGTGCATTTCTGATAGATATCACATGCCTAAGTGTTATACAGCATTTGAAGAGTTGGTTTACACTTTTGGCGGACCATGGTGGTTTGGTTTTATTCTGTTAAGCGTCCTCATCCTGTTAGCTCTTGTGCTTAGTGTTGCACGGATGAAGTATGTTACTGGAGACGAATTACCGACTCCTCTACCTGCTCGACAGGGCTCTCGTTTAGATCATTCCTTCCCTTTCCTGGAATCGTTGAATGAGGTTTTGGAAACAAATAGAAACGAGGAATCTCAGAGTCATGTGCATAGGATGTTTTTCATGGGGCCAAATACATTCAGTGAACCTTGGCATCTACCTCATTCCCCTCCAGAACAAGTCACAGAGATTGTGTATGAGGCTGCATTCAATAGATTTGTGGATGAGATAAATGGTTTAGCTGCTTATCAGTGGTGGGAAGGATCAGTCTACAGCATTCTTTGTGTGTTTGCCTATCCACTTGCCTGGTCATGGTTACAGAGTCGAAGGAAAAAGAAGTTACAACAACTTCGTGAATATGTTCGATCAGAATATGATCATTCTTGTTTGCGTTCTTGTCGTTCACGTGCACTGTATGAAGGACTCAAGGTAGCTGCAACTTCTGATTTAATGCTTGCATACGTGGACTTTTACCTTGGTGGAGATGAGAAAAGAGCTGGTCTTCCTCCCCGTCTTCATCAGCGATTTCCATTGTCATTAATTTTTGGAGGAGATGGAAGTTACATGGCACCTTTCCATCTTTGCAGTGATAACATTCTCACTAGCCTCATGAGTCAGAGTATTCCACCTACCATCTGGTATCGGCTTGTAGCTGGTCTAAACGCTCAACTGCGATTGGTTCGGTGTGGTCACTTAAAGTTAACTTTTGGTCATGTTATCAGCTGGCTTGAAACGCATGCTAACCCTACTCTCAGGCTATATGGCGTACATGTTGATCTTACCTGGTTTCAGCCTACAGCTTCTGGGTATAGCCAGTTTGGACTATTGATGTATGCAATCGACAATGAAAGTGTGCCACCAATACTGGAGGGTCAAGATGCATTGTTTCCGCGGGATTATCTGTCACGTACGCCAAGAAATCATTGGGAAAACTCATTTGAGCATTTGAGACTCATCGACCATTGGATGTCACAGAAAAGATTTTCTGGAGGCATTTTACACAGCAAGAACTTAAGGACGTTTAAAGAGAACAAAGCTATATGTTATCCATATTCTTTCGTAGTTTATAATGGCAAACCGGTTGGCCATCAGGATCTTGTCGGTTTGTTCATCTCCATTCTACTCTTGGGAGATTTTAGCATGGTCTTGCTCAGTTTGCTGCAGCTATATTCTATTTCACTGTTGAACTTTTTCCTAGTCTTATTTATTCTTCCTCTCGGTCTACTGTTCCCCTTCCCGGCTGGAATCAGTGCTTTGTTTAGTCGTGGACCTAAACGGGCAGCAGGTCTTGCGCGCATATATGCTTTATGGAATATCACATCCTTGATCAATGTCGGTGTTGCGTTTACGTGTGGATTGATTCATTATGCGACCCACTCGAGGAAAAAGCACTCCAACTTTCAGTCCTGGAATTTTAGCATGGATGAAAGTGAATGGTGGGTGCTTCCCTGCGGCTTGGCACTCTGTAAACTCGTGCAGTCGCGACTCATCGATTGCCATGTGGCGAACCAGGAAATTCAGGATCACTCATTGTACAGCAGCGACCCCGACGTTTTCTGGCAGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1438

Amino Acids

154.48

Weight (kDa)

6.17

Isoelectric Point (pI)

44.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8003 PF26010 795 - 869 5.1e-34 Domain of unknown function (DUF8003)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3813
AarI CACCTGC 2 cut(s) 959, 1979
AasI GACNNNNNNGTC 1 cut(s) 2220
Acc36I ACCTGC 4 cut(s) 959, 1979, 2761, 4019
AccB1I GGYRCC 2 cut(s) 497, 3313
AccBSI CCGCTC 1 cut(s) 184
AccI GTMKAC 2 cut(s) 3015, 3967
AccII CGCG 4 cut(s) 113, 3637, 4039, 4228
AclWI GGATC 5 cut(s) 1540, 1729, 3016, 3846, 4275
AcoI YGGCCR 1 cut(s) 3829
AcuI CTGAAG 2 cut(s) 497, 1658
AcvI CACGTG 1 cut(s) 3152
AcyI GRCGYC 1 cut(s) 1400
AdeI CACNNNGTG 1 cut(s) 2516
AfaI GTAC 3 cut(s) 3513, 3655, 4280
AfeI AGCGCT 1 cut(s) 2024
AfiI CCNNNNNNNGG 9 cut(s) 332, 1144, 2135, 2231, 2500, 2794, 3057, 3982, 4125
AflII CTTAAG 1 cut(s) 3757
AflIII ACRYGT 2 cut(s) 3514, 4094
AgeI ACCGGT 1 cut(s) 3823
AhdI GACNNNNNGTC 1 cut(s) 1013
AhlI ACTAGT 2 cut(s) 491, 659
AjuI GAANNNNNNNTTGG 4 cut(s) 2424, 2456, 2798, 2830
Alw21I GWGCWC 2 cut(s) 478, 3157
Alw26I GTCTC 6 cut(s) 206, 222, 1000, 2046, 2724, 3688
Alw44I GTGCAC 2 cut(s) 474, 3153
AlwI GGATC 5 cut(s) 1540, 1729, 3016, 3846, 4275
AlwNI CAGNNNCTG 2 cut(s) 974, 3548
Ama87I CYCGRG 1 cut(s) 4123
Aor51HI AGCGCT 1 cut(s) 2024
AoxI GGCC 6 cut(s) 1463, 1547, 1789, 2394, 2873, 3829
ApaLI GTGCAC 2 cut(s) 474, 3153
AseI ATTAAT 1 cut(s) 3284
AsiGI ACCGGT 1 cut(s) 3823
Asp700I GAANNNNTTC 5 cut(s) 886, 1239, 1623, 3105, 3935
AspA2I CCTAGG 1 cut(s) 1792
AspLEI GCGC 5 cut(s) 14, 83, 2025, 4039, 4041
AspS9I GGNCC 8 cut(s) 1463, 1526, 1547, 1759, 2126, 2642, 2873, 4013
AsuC2I CCSGG 2 cut(s) 400, 3983
AsuHPI GGTGA 2 cut(s) 643, 770
AsuII TTCGAA 3 cut(s) 117, 422, 1282
AvaI CYCGRG 1 cut(s) 4123
AvaII GGWCC 5 cut(s) 1526, 1759, 2126, 2642, 4013
AvrII CCTAGG 1 cut(s) 1792
BaeGI GKGCMC 2 cut(s) 478, 3157
BalI TGGCCA 1 cut(s) 3831
BanI GGYRCC 2 cut(s) 497, 3313
BanII GRGCYC 3 cut(s) 1279, 2479, 2768
BarI GAAGNNNNNNTAC 2 cut(s) 2314, 2346
BauI CACGAG 2 cut(s) 187, 4217
BbrPI CACGTG 1 cut(s) 3152
BbsI GAAGAC 3 cut(s) 503, 3239, 3251
Bbv12I GWGCWC 2 cut(s) 478, 3157
BceAI ACGGC 3 cut(s) 477, 1122, 1921
BclI TGATCA 2 cut(s) 3121, 4071
BcnI CCSGG 2 cut(s) 400, 3983
BcoDI GTCTC 6 cut(s) 206, 222, 1000, 2046, 2724, 3688
BcuI ACTAGT 2 cut(s) 491, 659
BfaI CTAG 9 cut(s) 72, 492, 549, 660, 1793, 1973, 2226, 3348, 3941
BfmI CTRYAG 6 cut(s) 975, 1752, 1986, 3016, 3540, 3905
BfoI RGCGCY 2 cut(s) 15, 2026
BfrI CTTAAG 1 cut(s) 3757
BfuAI ACCTGC 4 cut(s) 959, 1979, 2761, 4019
BglI GCCNNNNNGGC 1 cut(s) 2442
BglII AGATCT 2 cut(s) 1471, 1677
BlnI CCTAGG 1 cut(s) 1792
Bme18I GGWCC 5 cut(s) 1526, 1759, 2126, 2642, 4013
BmeRI GACNNNNNGTC 1 cut(s) 1013
BmeT110I CYCGRG 1 cut(s) 4123
BmgT120I GGNCC 8 cut(s) 1463, 1526, 1547, 1759, 2126, 2642, 2873, 4013
BmiI GGNNCC 6 cut(s) 159, 499, 1122, 1136, 2874, 3315
BmrI ACTGGG 2 cut(s) 1750, 2343
BmsI GCATC 8 cut(s) 982, 1333, 1793, 1915, 2281, 2334, 2908, 3613
BmuI ACTGGG 2 cut(s) 1750, 2343
BpiI GAAGAC 3 cut(s) 503, 3239, 3251
BplI GAGNNNNNCTC 2 cut(s) 1748, 1780
BpmI CTGGAG 5 cut(s) 2114, 2748, 2901, 3632, 3753
Bpu10I CCTNAGC 1 cut(s) 483
Bpu14I TTCGAA 3 cut(s) 117, 422, 1282
BpuEI CTTGAG 2 cut(s) 1388, 3155
BpuMI CCSGG 2 cut(s) 400, 3983
Bsa29I ATCGAT 1 cut(s) 4236
BsaAI YACGTR 5 cut(s) 603, 3152, 3208, 3653, 4095
BsaBI GATNNNNATC 2 cut(s) 2295, 2586
BsaHI GRCGYC 1 cut(s) 1400
BsaI GGTCTC 2 cut(s) 206, 222
BsaWI WCCGGW 3 cut(s) 305, 2387, 3823
BsaXI ACNNNNNCTCC 4 cut(s) 634, 664, 2539, 2569
Bsc4I CCNNNNNNNGG 9 cut(s) 332, 1144, 2135, 2231, 2500, 2794, 3057, 3982, 4125
Bse118I RCCGGY 3 cut(s) 154, 628, 3823
Bse3DI GCAATG 1 cut(s) 1384
Bse8I GATNNNNATC 2 cut(s) 2295, 2586
BseCI ATCGAT 1 cut(s) 4236
BseJI GATNNNNATC 2 cut(s) 2295, 2586
BseLI CCNNNNNNNGG 9 cut(s) 332, 1144, 2135, 2231, 2500, 2794, 3057, 3982, 4125
BseMI GCAATG 1 cut(s) 1384
BseMII CTCAG 5 cut(s) 474, 1405, 2855, 3511, 3910
BsePI GCGCGC 1 cut(s) 4037
BseRI GAGGAG 6 cut(s) 138, 885, 888, 2256, 2736, 3309
BseSI GKGCMC 2 cut(s) 478, 3157
BsgI GTGCAG 3 cut(s) 864, 1197, 4241
Bsh1236I CGCG 4 cut(s) 113, 3637, 4039, 4228
BshFI GGCC 6 cut(s) 1465, 1549, 1791, 2396, 2875, 3831
BshNI GGYRCC 2 cut(s) 497, 3313
BshTI ACCGGT 1 cut(s) 3823
BshVI ATCGAT 1 cut(s) 4236
BsiHKAI GWGCWC 2 cut(s) 478, 3157
BsiHKCI CYCGRG 1 cut(s) 4123
BsiSI CCGG 7 cut(s) 155, 306, 399, 629, 2388, 3824, 3983
BsiWI CGTACG 1 cut(s) 3653
BslFI GGGAC 4 cut(s) 143, 994, 2385, 2392
BslI CCNNNNNNNGG 9 cut(s) 332, 1144, 2135, 2231, 2500, 2794, 3057, 3982, 4125
BsmAI GTCTC 6 cut(s) 206, 222, 1000, 2046, 2724, 3688
BsmBI CGTCTC 1 cut(s) 2724
BsmFI GGGAC 4 cut(s) 143, 994, 2385, 2392
BsmI GAATGC 4 cut(s) 430, 2438, 2951, 3021
BsnI GGCC 6 cut(s) 1465, 1549, 1791, 2396, 2875, 3831
Bso31I GGTCTC 2 cut(s) 206, 222
BsoBI CYCGRG 1 cut(s) 4123
Bsp119I TTCGAA 3 cut(s) 117, 422, 1282
Bsp1286I GDGCHC 5 cut(s) 478, 1279, 2479, 2768, 3157
Bsp1407I TGTACA 1 cut(s) 4278
Bsp19I CCATGG 2 cut(s) 1614, 2645
Bsp68I TCGCGA 1 cut(s) 4228
BspANI GGCC 6 cut(s) 1465, 1549, 1791, 2396, 2875, 3831
BspCNI CTCAG 5 cut(s) 475, 1404, 2854, 3510, 3909
BspDI ATCGAT 1 cut(s) 4236
BspFNI CGCG 4 cut(s) 113, 3637, 4039, 4228
BspHI TCATGA 2 cut(s) 3354, 4310
BspLI GGNNCC 6 cut(s) 159, 499, 1122, 1136, 2874, 3315
BspMAI CTGCAG 3 cut(s) 979, 1990, 3909
BspMI ACCTGC 4 cut(s) 959, 1979, 2761, 4019
BspPI GGATC 5 cut(s) 1540, 1729, 3016, 3846, 4275
BspQI GCTCTTC 2 cut(s) 1230, 1284
BspT104I TTCGAA 3 cut(s) 117, 422, 1282
BspT107I GGYRCC 2 cut(s) 497, 3313
BspTI CTTAAG 1 cut(s) 3757
BspTNI GGTCTC 2 cut(s) 206, 222
BsrBI CCGCTC 1 cut(s) 184
BsrDI GCAATG 1 cut(s) 1384
BsrFI RCCGGY 3 cut(s) 154, 628, 3823
BsrGI TGTACA 1 cut(s) 4278
BssAI RCCGGY 3 cut(s) 154, 628, 3823
BssHII GCGCGC 1 cut(s) 4037
BssNI GRCGYC 1 cut(s) 1400
BssSI CACGAG 2 cut(s) 187, 4217
BssT1I CCWWGG 6 cut(s) 1110, 1614, 1792, 2645, 2894, 3220
Bst2BI CACGAG 2 cut(s) 187, 4217
Bst4CI ACNGT 9 cut(s) 412, 509, 1595, 2206, 2383, 2533, 3159, 3927, 3972
Bst6I CTCTTC 7 cut(s) 53, 877, 1230, 1284, 1677, 1771, 2613
BstACI GRCGYC 1 cut(s) 1400
BstAFI CTTAAG 1 cut(s) 3757
BstAPI GCANNNNNTGC 3 cut(s) 974, 1205, 4034
BstAUI TGTACA 1 cut(s) 4278
BstBAI YACGTR 5 cut(s) 603, 3152, 3208, 3653, 4095
BstBI TTCGAA 3 cut(s) 117, 422, 1282
BstC8I GCNNGC 8 cut(s) 430, 908, 1375, 2491, 3201, 3470, 3483, 4039
BstDEI CTNAG 9 cut(s) 483, 731, 1391, 2057, 2598, 2699, 2841, 3497, 3896
BstDSI CCRYGG 3 cut(s) 1614, 2645, 3635
BstFNI CGCG 4 cut(s) 113, 3637, 4039, 4228
BstH2I RGCGCY 2 cut(s) 15, 2026
BstHHI GCGC 5 cut(s) 14, 83, 2025, 4039, 4041
BstMAI GTCTC 6 cut(s) 206, 222, 1000, 2046, 2724, 3688
BstNSI RCATGY 3 cut(s) 2596, 3485, 3518
BstSFI CTRYAG 6 cut(s) 975, 1752, 1986, 3016, 3540, 3905
BstSLI GKGCMC 2 cut(s) 478, 3157
BstSNI TACGTA 1 cut(s) 603
BstUI CGCG 4 cut(s) 113, 3637, 4039, 4228
BstV2I GAAGAC 3 cut(s) 503, 3239, 3251
BstX2I RGATCY 5 cut(s) 1471, 1532, 1677, 1721, 3838
BstXI CCANNNNNNTGG 3 cut(s) 1117, 1769, 3051
BstYI RGATCY 5 cut(s) 1471, 1532, 1677, 1721, 3838
Bsu15I ATCGAT 1 cut(s) 4236
BsuRI GGCC 6 cut(s) 1465, 1549, 1791, 2396, 2875, 3831
BsuTUI ATCGAT 1 cut(s) 4236
BtgI CCRYGG 3 cut(s) 1614, 2645, 3635
BtsI GCAGTG 3 cut(s) 852, 1538, 3337
BtuMI TCGCGA 1 cut(s) 4228
BveI ACCTGC 4 cut(s) 959, 1979, 2761, 4019
Cac8I GCNNGC 8 cut(s) 430, 908, 1375, 2491, 3201, 3470, 3483, 4039
CaiI CAGNNNCTG 2 cut(s) 974, 3548
CciI TCATGA 2 cut(s) 3354, 4310
CfoI GCGC 5 cut(s) 14, 83, 2025, 4039, 4041
Cfr10I RCCGGY 3 cut(s) 154, 628, 3823
Cfr13I GGNCC 8 cut(s) 1463, 1526, 1547, 1759, 2126, 2642, 2873, 4013
Cfr42I CCGCGG 1 cut(s) 3638
ClaI ATCGAT 1 cut(s) 4236
CseI GACGC 2 cut(s) 1389, 2662
CsiI ACCWGGT 1 cut(s) 3527
Csp6I GTAC 3 cut(s) 3512, 3654, 4279
CspAI ACCGGT 1 cut(s) 3823
CviQI GTAC 3 cut(s) 3512, 3654, 4279
DdeI CTNAG 9 cut(s) 483, 731, 1391, 2057, 2598, 2699, 2841, 3497, 3896
DraI TTTAAA 1 cut(s) 3769
DraIII CACNNNGTG 1 cut(s) 2516
DrdI GACNNNNNNGTC 1 cut(s) 2220
DriI GACNNNNNGTC 1 cut(s) 1013
DseDI GACNNNNNNGTC 1 cut(s) 2220
EaeI YGGCCR 1 cut(s) 3829
Eam1104I CTCTTC 7 cut(s) 53, 877, 1230, 1284, 1677, 1771, 2613
Eam1105I GACNNNNNGTC 1 cut(s) 1013
EarI CTCTTC 7 cut(s) 53, 877, 1230, 1284, 1677, 1771, 2613
EciI GGCGGA 4 cut(s) 141, 150, 609, 2655
Eco105I TACGTA 1 cut(s) 603
Eco130I CCWWGG 6 cut(s) 1110, 1614, 1792, 2645, 2894, 3220
Eco147I AGGCCT 1 cut(s) 2396
Eco24I GRGCYC 3 cut(s) 1279, 2479, 2768
Eco31I GGTCTC 2 cut(s) 206, 222
Eco32I GATATC 3 cut(s) 925, 1096, 2588
Eco47I GGWCC 5 cut(s) 1526, 1759, 2126, 2642, 4013
Eco47III AGCGCT 1 cut(s) 2024
Eco57I CTGAAG 2 cut(s) 497, 1658
Eco72I CACGTG 1 cut(s) 3152
Eco88I CYCGRG 1 cut(s) 4123
EcoO109I RGGNCCY 2 cut(s) 1547, 2126
EcoRI GAATTC 3 cut(s) 241, 365, 1312
EcoRV GATATC 3 cut(s) 925, 1096, 2588
EcoT14I CCWWGG 6 cut(s) 1110, 1614, 1792, 2645, 2894, 3220
EcoT22I ATGCAT 2 cut(s) 2296, 3628
EcoT38I GRGCYC 3 cut(s) 1279, 2479, 2768
ErhI CCWWGG 6 cut(s) 1110, 1614, 1792, 2645, 2894, 3220
Esp3I CGTCTC 1 cut(s) 2724
FaqI GGGAC 4 cut(s) 143, 994, 2385, 2392
FauI CCCGC 3 cut(s) 189, 463, 3630
FbaI TGATCA 2 cut(s) 3121, 4071
FblI GTMKAC 2 cut(s) 3015, 3967
FriOI GRGCYC 3 cut(s) 1279, 2479, 2768
FspBI CTAG 9 cut(s) 72, 492, 549, 660, 1793, 1973, 2226, 3348, 3941
GlaI GCGC 5 cut(s) 13, 82, 2024, 4038, 4040
GsuI CTGGAG 5 cut(s) 2114, 2748, 2901, 3632, 3753
HaeII RGCGCY 2 cut(s) 15, 2026
HaeIII GGCC 6 cut(s) 1465, 1549, 1791, 2396, 2875, 3831
HapII CCGG 7 cut(s) 155, 306, 399, 629, 2388, 3824, 3983
HgaI GACGC 2 cut(s) 1389, 2662
HhaI GCGC 5 cut(s) 14, 83, 2025, 4039, 4041
Hin1I GRCGYC 1 cut(s) 1400
Hin6I GCGC 5 cut(s) 12, 81, 2023, 4037, 4039
HinP1I GCGC 5 cut(s) 12, 81, 2023, 4037, 4039
HincII GTYRAC 2 cut(s) 352, 3447
HindII GTYRAC 2 cut(s) 352, 3447
HindIII AAGCTT 1 cut(s) 247
HpaI GTTAAC 2 cut(s) 352, 3447
HpaII CCGG 7 cut(s) 155, 306, 399, 629, 2388, 3824, 3983
HphI GGTGA 2 cut(s) 643, 770
Hpy99I CGWCG 6 cut(s) 95, 509, 584, 819, 2069, 4298
HpyCH4III ACNGT 9 cut(s) 412, 509, 1595, 2206, 2383, 2533, 3159, 3927, 3972
HpyCH4IV ACGT 7 cut(s) 602, 3151, 3207, 3652, 3764, 4094, 4296
HpyF3I CTNAG 9 cut(s) 483, 731, 1391, 2057, 2598, 2699, 2841, 3497, 3896
HpySE526I ACGT 7 cut(s) 602, 3151, 3207, 3652, 3764, 4094, 4296
Hsp92I GRCGYC 1 cut(s) 1400
HspAI GCGC 5 cut(s) 12, 81, 2023, 4037, 4039
Ksp22I TGATCA 2 cut(s) 3121, 4071
KspAI GTTAAC 2 cut(s) 352, 3447
KspI CCGCGG 1 cut(s) 3638
LguI GCTCTTC 2 cut(s) 1230, 1284
LmnI GCTCC 5 cut(s) 288, 579, 1134, 2020, 2503
LweI GCATC 8 cut(s) 982, 1333, 1793, 1915, 2281, 2334, 2908, 3613
MabI ACCWGGT 1 cut(s) 3527
MaeI CTAG 9 cut(s) 72, 492, 549, 660, 1793, 1973, 2226, 3348, 3941
MaeII ACGT 7 cut(s) 602, 3151, 3207, 3652, 3764, 4094, 4296
MbiI CCGCTC 1 cut(s) 184
MfeI CAATTG 2 cut(s) 723, 1347
MflI RGATCY 5 cut(s) 1471, 1532, 1677, 1721, 3838
MhlI GDGCHC 5 cut(s) 478, 1279, 2479, 2768, 3157
MlsI TGGCCA 1 cut(s) 3831
MluNI TGGCCA 1 cut(s) 3831
MlyI GAGTC 7 cut(s) 2737, 2854, 3076, 3162, 3367, 3690, 4224
MmeI TCCRAC 3 cut(s) 114, 687, 4164
Mox20I TGGCCA 1 cut(s) 3831
Mph1103I ATGCAT 2 cut(s) 2296, 3628
MroXI GAANNNNTTC 5 cut(s) 886, 1239, 1623, 3105, 3935
MscI TGGCCA 1 cut(s) 3831
MslI CAYNNNNRTG 2 cut(s) 1416, 2006
Msp20I TGGCCA 1 cut(s) 3831
MspA1I CMGCKG 2 cut(s) 3468, 3637
MspCI CTTAAG 1 cut(s) 3757
MspI CCGG 7 cut(s) 155, 306, 399, 629, 2388, 3824, 3983
MunI CAATTG 2 cut(s) 723, 1347
Mva1269I GAATGC 4 cut(s) 430, 2438, 2951, 3021
MvnI CGCG 4 cut(s) 113, 3637, 4039, 4228
NciI CCSGG 2 cut(s) 400, 3983
NcoI CCATGG 2 cut(s) 1614, 2645
NlaIV GGNNCC 6 cut(s) 159, 499, 1122, 1136, 2874, 3315
NmuCI GTSAC 6 cut(s) 2527, 2545, 2926, 3434, 3648, 3714
NruI TCGCGA 1 cut(s) 4228
NsiI ATGCAT 2 cut(s) 2296, 3628
NspI RCATGY 3 cut(s) 2596, 3485, 3518
NspV TTCGAA 3 cut(s) 117, 422, 1282
PaeI GCATGC 1 cut(s) 3485
PaeR7I CTCGAG 1 cut(s) 4123
PagI TCATGA 2 cut(s) 3354, 4310
PaqCI CACCTGC 2 cut(s) 959, 1979
PauI GCGCGC 1 cut(s) 4037
PceI AGGCCT 1 cut(s) 2396
PciI ACATGT 1 cut(s) 3514
PciSI GCTCTTC 2 cut(s) 1230, 1284
PctI GAATGC 4 cut(s) 430, 2438, 2951, 3021
PdmI GAANNNNTTC 5 cut(s) 886, 1239, 1623, 3105, 3935
Pfl23II CGTACG 1 cut(s) 3653
PfoI TCCNGGA 2 cut(s) 2793, 4151
PinAI ACCGGT 1 cut(s) 3823
PleI GAGTC 7 cut(s) 2737, 2853, 3075, 3162, 3366, 3690, 4224
PmaCI CACGTG 1 cut(s) 3152
PmlI CACGTG 1 cut(s) 3152
PpsI GAGTC 7 cut(s) 2737, 2853, 3075, 3162, 3366, 3690, 4224
Ppu21I YACGTR 5 cut(s) 603, 3152, 3208, 3653, 4095
PpuMI RGGWCCY 1 cut(s) 2126
PscI ACATGT 1 cut(s) 3514
PshBI ATTAAT 1 cut(s) 3284
PsiI TTATAA 1 cut(s) 3813
Psp5II RGGWCCY 1 cut(s) 2126
PspCI CACGTG 1 cut(s) 3152
PspLI CGTACG 1 cut(s) 3653
PspN4I GGNNCC 6 cut(s) 159, 499, 1122, 1136, 2874, 3315
PspPI GGNCC 8 cut(s) 1463, 1526, 1547, 1759, 2126, 2642, 2873, 4013
PspPPI RGGWCCY 1 cut(s) 2126
PspXI VCTCGAGB 1 cut(s) 4123
PstI CTGCAG 3 cut(s) 979, 1990, 3909
PstNI CAGNNNCTG 2 cut(s) 974, 3548
PsuI RGATCY 5 cut(s) 1471, 1532, 1677, 1721, 3838
PteI GCGCGC 1 cut(s) 4037
PvuII CAGCTG 1 cut(s) 3468
RruI TCGCGA 1 cut(s) 4228
RsaI GTAC 3 cut(s) 3513, 3655, 4280
RsaNI GTAC 3 cut(s) 3512, 3654, 4279
RseI CAYNNNNRTG 2 cut(s) 1416, 2006
SacII CCGCGG 1 cut(s) 3638
SapI GCTCTTC 2 cut(s) 1230, 1284
Sau96I GGNCC 8 cut(s) 1463, 1526, 1547, 1759, 2126, 2642, 2873, 4013
SchI GAGTC 7 cut(s) 2737, 2854, 3076, 3162, 3367, 3690, 4224
SduI GDGCHC 5 cut(s) 478, 1279, 2479, 2768, 3157
SexAI ACCWGGT 1 cut(s) 3527
SfaNI GCATC 8 cut(s) 982, 1333, 1793, 1915, 2281, 2334, 2908, 3613
SfcI CTRYAG 6 cut(s) 975, 1752, 1986, 3016, 3540, 3905
Sfr274I CTCGAG 1 cut(s) 4123
Sfr303I CCGCGG 1 cut(s) 3638
SfuI TTCGAA 3 cut(s) 117, 422, 1282
SgrBI CCGCGG 1 cut(s) 3638
SinI GGWCC 5 cut(s) 1526, 1759, 2126, 2642, 4013
SlaI CTCGAG 1 cut(s) 4123
SmiMI CAYNNNNRTG 2 cut(s) 1416, 2006
SmlI CTYRAG 4 cut(s) 1367, 3170, 3757, 4123
SmoI CTYRAG 4 cut(s) 1367, 3170, 3757, 4123
SnaBI TACGTA 1 cut(s) 603
SpeI ACTAGT 2 cut(s) 491, 659
SphI GCATGC 1 cut(s) 3485
SseBI AGGCCT 1 cut(s) 2396
SspI AATATT 1 cut(s) 1522
SspMI CTAG 9 cut(s) 72, 492, 549, 660, 1793, 1973, 2226, 3348, 3941
StuI AGGCCT 1 cut(s) 2396
StyI CCWWGG 6 cut(s) 1110, 1614, 1792, 2645, 2894, 3220
TaaI ACNGT 9 cut(s) 412, 509, 1595, 2206, 2383, 2533, 3159, 3927, 3972
TaiI ACGT 7 cut(s) 605, 3154, 3210, 3655, 3767, 4097, 4299
TaqII GACCGA 1 cut(s) 3953
TatI WGTACW 1 cut(s) 4278
TauI GCSGC 3 cut(s) 166, 169, 4200
TseFI GTSAC 6 cut(s) 2527, 2545, 2926, 3434, 3648, 3714
Tsp45I GTSAC 6 cut(s) 2527, 2545, 2926, 3434, 3648, 3714
TspGWI ACGGA 3 cut(s) 1393, 2082, 2725
Vha464I CTTAAG 1 cut(s) 3757
VneI GTGCAC 2 cut(s) 474, 3153
VpaK11BI GGWCC 5 cut(s) 1526, 1759, 2126, 2642, 4013
VspI ATTAAT 1 cut(s) 3284
XceI RCATGY 3 cut(s) 2596, 3485, 3518
XcmI CCANNNNNNNNNTGG 3 cut(s) 3378, 3666, 3868
XhoI CTCGAG 1 cut(s) 4123
XmaJI CCTAGG 1 cut(s) 1792
XmiI GTMKAC 2 cut(s) 3015, 3967
XmnI GAANNNNTTC 5 cut(s) 886, 1239, 1623, 3105, 3935
XspI CTAG 9 cut(s) 72, 492, 549, 660, 1793, 1973, 2226, 3348, 3941
Zsp2I ATGCAT 2 cut(s) 2296, 3628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.