MD15G1089200.v1.1

glycine-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002500
Physical Location & Seq
Forward (+)
6207858 .. 6217372
9515 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1089200.v1.1.491

Sequence Viewer

Length: 4353 bp
ATGGCTCGCCTCGGCCTTCCCACCCTCCTCATCGCATTCCTCTCCGCCCTGATAGCCTTAACTGCAAACCCTAGCCACCTCCTCCTCGCTTCCGTAGACGATGAGTTTACGATAACCGATTCCTCCACCTCCGATTCCTATCTCTTCCACCAAGACTACTCGCCGCCGGCTCCGCCACCTCCGCCGCCGCGCCCGCCGTCGGTATCGTGCACCGATGACCTCGGTGGCGTCGGCTCTCTGGACGCCACGTGTCAGATAGTCTCCGATTCGAACCTCACCAGCGACGTGTACATAACAGGGAAGGGCAGCTTTTATATCTTGCCCGGAGTGAGATTCAGCTGCGCGATTCCTGGCTGCGCGATAATTATTAACATTACCGGTAACTTTAGCTTAGGCAGCAACGCGTCGCTTTTGGCTGGGGCTTTCGAGCTCACGGCGCACAATGCCAGCTTTCTCGAGGGCTCGGCGCTGAACACGACGGCGTTGGCGGGAAAGCCGCCGCCTCAGACGAGTGGGACTCCGCAGGGGATAGATGGGGCGGGAGGGGGACATGGAGGGCGGGGGGCGTCTTGTTTGGTGGACAAGAGGAAGCTTCCCGAGGACGTGTGGGGAGGGGATGCGTACTCGTGGTCGTCACTGCAAAGGCCGGCTAGTTTTGGGAGCCGAGGCGGTTCGACCAGTAGGGAGGTAGATTATGGAGGTTTAGGGGGAGGGAGGGTTAGGCTGCAAGTGAGGGAATTTCTGGTGGTGGAGGGGAGGGTTTTGGCTGAAGGAGGTGGCGGAGGGAATAGAGGTGGCGGTGGTTCTGGTGGCAGCATCTTCATTAAGGCTTATAAAATGACTGGTATTGGCAGGATAAGTGCTTGTGGTGGTGATGGTTATGCTGGTGGTGGTGGTGGAAGAGTGTCAGTTGATGTCTATAGCAGGCATGATGACCCCCAAATTTTTGTGCATGGAGGAAATAGCTATGCTTGTCCAGAAAATGCAGGCGGGGCTGGGACTTTATATGATGCAGTTCCTCGAAGCCTCATTGTTAGCAATCATAACAAGTCAACAGATACAGAAACACTTCTTTTGGAGTTTCCTTATCAGCCTCTTTGGACAAATGTTTATATTCAGAATAAGGCTAGGGCCACTGTCCCATTGCTTTGGAGTCGTGTCCAGGTGCAAGGACAGATAAGCCTTTTGAGTGACGGTATGTTAAGCTTTGGACTTGAACATTATGCTTCATCAGAATTTGAGTTATTGGCTGAAGAATTGTTGATGAGCGACTCTGTAATTAAGGTGTATGGGGCCTTACGTATGACTGTAAAAATGTTCTTGATGTGGAATTCCAAGATACTTATAGATGGTGGGGGGGAAGAAGCTGTTGAGACATCCTTGCTCGAGGCTAGCAACTTAGTTGTTCTCAGGGGATCATCTGTGATACACTCTAATGCAAATCTGGGAGTTCATGGACAAGGTTTATTGAACTTATCAGGACCTGGAGATGGGATTCAAGCACAACGTCTGGTTCTATCGCTATTCTACAGTATTCATGTTGGGCCTGGATCTGTTTTGCGTGGTCCTTTAGAGAATGCCACAACTGATTCTGTGACACCAAAGCTTTATTGTGAAAACGAAGATTGTCCCTATGAGCTACTTCATCCACCTGAAGATTGCAATGTGAACTCATCCCTGTCCTTTACTCTTATGGTATGCCGAGTTGAAGATATCATTATTGAAGGCCTCATAGAAGGATCTGTTGTTCATTTTCACCGGGCAAGGACCATTGCTATCCATTCTTCTGGAGAAATAAGTGCATCAGGAATGGGTTGTACTGGTGGTATTGGCAGTGGCAACATATTAGGCAATGGAATTGGCAGTGGTGGGGGGCATGGAGGTAAAGGTGGGGCTGCATGTTATAATGACAGTTGTGTTGAGGGTGGAATCTCATATGGGAATGTGAAGTTGCCTTGTGAACTTGGTAGTGGAAGTGGATATGACTTTTCAGCTGGCTTAACTGCTGGTGGTGGTATTATCGTAATGGGTTCTTTAGAACATCCCTTGTCAAGTTTGTCTGTTGAAGGCTCGCTGACAGCTGATGGTGAAAGTTTTGAAGGGACTGCTGTGAAGGAAAAGTTTGCCCTTGCTGATAATACAACTGGAGGCCCTGGTGGTGGGTCTGGTGGAACTATACTTTTGTTCTTGCGAACACTAGACCTTGGTGAGACTGCCATTCTTTCAAGTGTTGGGGGATATGGTAGTTCAATTGGCGGTGGTGGAGGTGGTGGTGGAAGGATTCACTTTCATTGGTCAGATATTCCCACTGGAGATGTGTATCAGCCCATTGCAAGTGTGGACGGAAGCATCCTTGCAGGGGGAGGGGTGGGTGGAGACCAGGGTGGTGCTGGAGAAAATGGAACAGTGACGGGTAAAGCTTGCCCAAAAGGTCTTTATGGGACCTTTTGTGGGGCATGTCCAGTTGGCACTTATAAGAATGCTATTGGGTCAGATAGAGCACTTTGTCATCATTGTCCTGCTACTGAGCTTCCCCCTCGTGCAATTTATATATCTGTCCGAGGTGGTGTTGCAGAGGCTCCTTGTCCTTACAAATGCATTTCAGACAGATATCACATGCCACACTGTTATACTGCTCTTGAAGAATTGATTTACACATTTGGTGGGCCTTGGCTGTTTGGTCTTCTTCTGATTGGGCTCCTCATCCTGTTGGCTTTGGTGCTCAGTGTTGCACGTATGAAATTGGTTGGTGTTGATGAATTACCAGGCCCTGCTCCCACTCAACATGGCTCACAAATAGACCACTCTTTCCCTTTCCTGGAGTCATTGAATGAGGTTTTAGAAACAAACAGGGCTGAGGAGTCACAGAGCCATGTGCACCGGATGTATTTTATGGGTCCTAATACTTTTGGGGATCCTTGGCATTTGACTCACACTCCTCCAGAACAAATAAAAGAGATTGTATATGAGGGGCCATTCAATACATTTGTCGATGAGATTAATTCCATAGCCACATATCAATGGTGGGAGGGAGCAATGCACAGCATTCTCTCTGTTCTTGCATACCCCCTTGCATGGTCATGGCAGCAGTGGCGCCGAAGATTGAAACTGCAACGTCTGCGTGAATTCGTTCGATCAGAGTATGATCATGCCTGCTTAAGGTCTTGCCGTTCGCGTGCTTTATATGAAGGGATTAAGGTAGCTGCAACTTCTGATTTAATGCTAGCATATATGGACTTCTTCCTTGGTGGTGATGAAAAGAGAACTGATCTTCCCCCTCGTTTACATCAAAGGTTTCCGATTTCATTACCTTTTGGAGGTGATGGAAGTTATATGGCCCCTTTCTCACTTCACAGTGATAACATTGTTACAAGCCTCATGAGTCAGGCAGTCCCGCCGACCTCATGGTACCGTATGGTTGCTGGTTTGAATGCACAGTTGCGCTTAGTTTGCCGTGGGCGGCTAAGAGTTACACTTCAGCCTGTCCTTAGGTGGCTAGAAAATTATGCCAATCCTGCTTTGAAGATTTATGGTGTACGTGTTGATCTTGCCTGGTTTCAGGCTACATCTTGTGGTTATTGTCATTATGGGCTCGTGGTGGATGCTTTTGAAGAAGACAGTGAACCTGCCTCTGTTGGAAGCATTGATGGAGCAATACGAACTGAAGAATCACGTGCAAATTACATAGAAGATTCATCTGGTCATTTGAGACAATCACTCCTAAACCAGTCTCACAGAAGTGAAAATTTTATGAGGCCAAAAAGAGCATGTGGAGGGATTATAGATGCCAACAACATACAAAAGCTTGAGGAAAAGAGAGATATGTTTTATCTTCTCTCTTTTATACTTCATAATACCAAACCTGTTGGACACCAGGATCTTGTTGGTTTAGTTATCTCAATGCTTCTTTTAGGAGATTTTAGCTTAGCCTTGCTTACATTACTCCAGCTGTATTCATTTTCATTGGTGGACGTCTTTCTGGTTCTATTTATTTTACCCCTTGGCATTCTTCTCCCGTTTCCTGCTGGTATCAATGCTCTATTCAGTCATGGACCCAGACGTTCTGCTGGCCTTGCACGTCTACATGCTTTGTGGAACCTTACATCCTTGATTAATGTTGTGGTTGCTTTTGTTTGTGGTTACGTTCATTATAACACTCAATCATCAAGTAGAAAACATCCCTTTCAACCCTGGAGTGTTAGCATGGACGATAGTGAATGGTGGATTTTTCCTGCCGGTCTGCTGCTTTGTAAAGCTTTTCAGTCGCAGCTAATCAATTGGCATGTTGCGAATCTGGAGATTCAAGACCGTTCGTTGTACAGCAATGATTTTGAGCTCTTCTGGCAGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1451

Amino Acids

155.04

Weight (kDa)

5.59

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8003 PF26010 803 - 877 3.8e-35 Domain of unknown function (DUF8003)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 834, 1905, 2475, 4152
AatII GACGTC 1 cut(s) 3975
Acc36I ACCTGC 1 cut(s) 3634
Acc65I GGTACC 1 cut(s) 3408
AccB1I GGYRCC 2 cut(s) 3093, 3408
AccI GTMKAC 2 cut(s) 96, 4081
AccII CGCG 5 cut(s) 190, 344, 359, 404, 3175
AclWI GGATC 6 cut(s) 1423, 1558, 1747, 2909, 2922, 3885
AcsI RAATTY 6 cut(s) 737, 942, 1235, 1330, 3125, 3745
AcuI CTGAAG 5 cut(s) 789, 1272, 1674, 3461, 3684
AcvI CACGTG 2 cut(s) 249, 3674
AcyI GRCGYC 5 cut(s) 228, 243, 566, 3094, 3972
AfaI GTAC 6 cut(s) 290, 623, 1819, 3410, 3537, 4319
AfiI CCNNNNNNNGG 9 cut(s) 199, 1490, 2159, 2359, 2451, 2818, 3075, 3159, 3317
AflII CTTAAG 1 cut(s) 3157
AflIII ACRYGT 5 cut(s) 248, 285, 402, 603, 3538
AgeI ACCGGT 1 cut(s) 377
AjiI CACGTC 3 cut(s) 286, 604, 4079
AleI CACNNNNGTG 1 cut(s) 3738
AloI GAACNNNNNNTCC 6 cut(s) 1731, 1763, 3256, 3288, 4088, 4120
Alw21I GWGCWC 6 cut(s) 212, 432, 2503, 2724, 2880, 4338
Alw26I GTCTC 6 cut(s) 265, 1367, 2204, 2370, 3703, 3735
Alw44I GTGCAC 2 cut(s) 208, 2876
AlwI GGATC 6 cut(s) 1423, 1558, 1747, 2909, 2922, 3885
AlwNI CAGNNNCTG 2 cut(s) 1484, 2771
Ama87I CYCGRG 3 cut(s) 455, 596, 1385
ApaLI GTGCAC 2 cut(s) 208, 2876
ApoI RAATTY 6 cut(s) 737, 942, 1235, 1330, 3125, 3745
AseI ATTAAT 2 cut(s) 3000, 4113
AsiGI ACCGGT 1 cut(s) 377
Asp700I GAANNNNTTC 2 cut(s) 1068, 3129
Asp718I GGTACC 1 cut(s) 3408
AspLEI GCGC 7 cut(s) 192, 344, 359, 439, 469, 3096, 3444
AsuC2I CCSGG 2 cut(s) 324, 1760
AsuHPI GGTGA 7 cut(s) 268, 884, 1748, 2099, 2219, 3263, 3332
AsuII TTCGAA 1 cut(s) 269
AsuNHI GCTAGC 2 cut(s) 1391, 3223
AvaI CYCGRG 3 cut(s) 455, 596, 1385
AvaII GGWCC 6 cut(s) 1481, 1565, 1767, 2442, 2897, 4052
AxyI CCTNAGG 1 cut(s) 3488
BaeGI GKGCMC 2 cut(s) 212, 2880
BamHI GGATCC 1 cut(s) 2914
BanI GGYRCC 2 cut(s) 3093, 3408
BanII GRGCYC 5 cut(s) 432, 464, 2700, 3594, 4338
BauI CACGAG 3 cut(s) 625, 2538, 3593
BbrPI CACGTG 2 cut(s) 249, 3674
BbsI GAAGAC 2 cut(s) 2675, 3621
Bbv12I GWGCWC 6 cut(s) 212, 432, 2503, 2724, 2880, 4338
BbvCI CCTCAGC 1 cut(s) 2856
BccI CCATC 7 cut(s) 527, 869, 1343, 1484, 2078, 3317, 3641
BceAI ACGGC 5 cut(s) 181, 450, 495, 3153, 3438
BclI TGATCA 1 cut(s) 3145
BcnI CCSGG 2 cut(s) 324, 1760
BcoDI GTCTC 6 cut(s) 265, 1367, 2204, 2370, 3703, 3735
BfaI CTAG 7 cut(s) 72, 651, 1128, 1392, 2198, 3224, 3497
BfmI CTRYAG 2 cut(s) 919, 1528
BfoI RGCGCY 2 cut(s) 470, 3097
BfrI CTTAAG 1 cut(s) 3157
BfuAI ACCTGC 1 cut(s) 3634
BlpI GCTNAGC 1 cut(s) 3925
Bme18I GGWCC 6 cut(s) 1481, 1565, 1767, 2442, 2897, 4052
BmeT110I CYCGRG 3 cut(s) 455, 596, 1385
BmgBI CACGTC 3 cut(s) 286, 604, 4079
BmsI GCATC 7 cut(s) 607, 825, 1000, 1811, 2358, 3592, 3775
BmtI GCTAGC 2 cut(s) 1395, 3227
BpiI GAAGAC 2 cut(s) 2675, 3621
BplI GAGNNNNNCTC 2 cut(s) 502, 534
Bpu10I CCTNAGC 2 cut(s) 391, 2856
Bpu1102I GCTNAGC 1 cut(s) 3925
Bpu14I TTCGAA 1 cut(s) 269
BpuEI CTTGAG 1 cut(s) 3827
BpuMI CCSGG 2 cut(s) 324, 1760
BsaAI YACGTR 5 cut(s) 249, 1301, 2735, 3539, 3674
BsaBI GATNNNNATC 2 cut(s) 138, 2319
BsaHI GRCGYC 5 cut(s) 228, 243, 566, 3094, 3972
BsaI GGTCTC 1 cut(s) 2370
BsaWI WCCGGW 2 cut(s) 377, 2880
BsaXI ACNNNNNCTCC 6 cut(s) 702, 732, 2920, 2950, 3702, 3732
Bsc4I CCNNNNNNNGG 9 cut(s) 199, 1490, 2159, 2359, 2451, 2818, 3075, 3159, 3317
Bse118I RCCGGY 4 cut(s) 166, 377, 646, 4235
Bse1I ACTGG 7 cut(s) 678, 847, 1825, 2149, 2314, 2462, 3727
Bse21I CCTNAGG 1 cut(s) 3488
Bse3DI GCAATG 7 cut(s) 1142, 1669, 1770, 1858, 2328, 3042, 4330
Bse8I GATNNNNATC 2 cut(s) 138, 2319
BseJI GATNNNNATC 2 cut(s) 138, 2319
BseLI CCNNNNNNNGG 9 cut(s) 199, 1490, 2159, 2359, 2451, 2818, 3075, 3159, 3317
BseMI GCAATG 7 cut(s) 1142, 1669, 1770, 1858, 2328, 3042, 4330
BseMII CTCAG 5 cut(s) 518, 1423, 2517, 2737, 2847
BseNI ACTGG 7 cut(s) 678, 847, 1825, 2149, 2314, 2462, 3727
BseRI GAGGAG 6 cut(s) 17, 71, 74, 2690, 2873, 2928
BseSI GKGCMC 2 cut(s) 212, 2880
BseYI CCCAGC 2 cut(s) 416, 995
Bsh1236I CGCG 5 cut(s) 190, 344, 359, 404, 3175
BshNI GGYRCC 2 cut(s) 3093, 3408
BshTI ACCGGT 1 cut(s) 377
BsiHKAI GWGCWC 6 cut(s) 212, 432, 2503, 2724, 2880, 4338
BsiHKCI CYCGRG 3 cut(s) 455, 596, 1385
BsiSI CCGG 7 cut(s) 167, 324, 378, 647, 1759, 2881, 4236
BslFI GGGAC 8 cut(s) 529, 561, 1012, 1124, 1614, 2116, 2455, 3377
BslI CCNNNNNNNGG 9 cut(s) 199, 1490, 2159, 2359, 2451, 2818, 3075, 3159, 3317
BsmAI GTCTC 6 cut(s) 265, 1367, 2204, 2370, 3703, 3735
BsmFI GGGAC 8 cut(s) 529, 561, 1012, 1124, 1614, 2116, 2455, 3377
BsmI GAATGC 6 cut(s) 35, 1582, 2485, 3045, 3436, 4005
Bso31I GGTCTC 1 cut(s) 2370
BsoBI CYCGRG 3 cut(s) 455, 596, 1385
Bsp119I TTCGAA 1 cut(s) 269
Bsp1286I GDGCHC 9 cut(s) 212, 432, 464, 2503, 2700, 2724, 2880, 3594, 4338
Bsp1407I TGTACA 2 cut(s) 288, 4317
Bsp143I GATC 9 cut(s) 1415, 1550, 1739, 2914, 3134, 3145, 3268, 3544, 3877
Bsp1720I GCTNAGC 1 cut(s) 3925
BspCNI CTCAG 5 cut(s) 517, 1422, 2518, 2736, 2848
BspFNI CGCG 5 cut(s) 190, 344, 359, 404, 3175
BspHI TCATGA 1 cut(s) 3378
BspMI ACCTGC 1 cut(s) 3634
BspOI GCTAGC 2 cut(s) 1395, 3227
BspPI GGATC 6 cut(s) 1423, 1558, 1747, 2909, 2922, 3885
BspQI GCTCTTC 1 cut(s) 4343
BspT104I TTCGAA 1 cut(s) 269
BspT107I GGYRCC 2 cut(s) 3093, 3408
BspTI CTTAAG 1 cut(s) 3157
BspTNI GGTCTC 1 cut(s) 2370
BsrDI GCAATG 7 cut(s) 1142, 1669, 1770, 1858, 2328, 3042, 4330
BsrFI RCCGGY 4 cut(s) 166, 377, 646, 4235
BsrGI TGTACA 2 cut(s) 288, 4317
BsrI ACTGG 7 cut(s) 678, 847, 1825, 2149, 2314, 2462, 3727
BssAI RCCGGY 4 cut(s) 166, 377, 646, 4235
BssMI GATC 9 cut(s) 1415, 1550, 1739, 2914, 3134, 3145, 3268, 3544, 3877
BssNI GRCGYC 5 cut(s) 228, 243, 566, 3094, 3972
BssSI CACGAG 3 cut(s) 625, 2538, 3593
BssT1I CCWWGG 5 cut(s) 2203, 2669, 2918, 3244, 4000
Bst2BI CACGAG 3 cut(s) 625, 2538, 3593
Bst6I CTCTTC 3 cut(s) 149, 895, 4343
BstACI GRCGYC 5 cut(s) 228, 243, 566, 3094, 3972
BstAFI CTTAAG 1 cut(s) 3157
BstAPI GCANNNNNTGC 1 cut(s) 3118
BstAUI TGTACA 2 cut(s) 288, 4317
BstBAI YACGTR 5 cut(s) 249, 1301, 2735, 3539, 3674
BstBI TTCGAA 1 cut(s) 269
BstDSI CCRYGG 1 cut(s) 3454
BstENI CCTNNNNNAGG 2 cut(s) 3157, 3315
BstFNI CGCG 5 cut(s) 190, 344, 359, 404, 3175
BstH2I RGCGCY 2 cut(s) 470, 3097
BstHHI GCGC 7 cut(s) 192, 344, 359, 439, 469, 3096, 3444
BstKTI GATC 9 cut(s) 1418, 1553, 1742, 2917, 3137, 3148, 3271, 3547, 3880
BstMAI GTCTC 6 cut(s) 265, 1367, 2204, 2370, 3703, 3735
BstMBI GATC 9 cut(s) 1415, 1550, 1739, 2914, 3134, 3145, 3268, 3544, 3877
BstNSI RCATGY 6 cut(s) 1902, 2460, 2620, 3771, 4088, 4286
BstSFI CTRYAG 2 cut(s) 919, 1528
BstSLI GKGCMC 2 cut(s) 212, 2880
BstSNI TACGTA 1 cut(s) 1301
BstUI CGCG 5 cut(s) 190, 344, 359, 404, 3175
BstV2I GAAGAC 2 cut(s) 2675, 3621
BstX2I RGATCY 4 cut(s) 1550, 1739, 2914, 3877
BstXI CCANNNNNNTGG 2 cut(s) 1149, 1787
BstYI RGATCY 4 cut(s) 1550, 1739, 2914, 3877
Bsu36I CCTNAGG 1 cut(s) 3488
BtgI CCRYGG 1 cut(s) 3454
BtgZI GCGATG 1 cut(s) 16
BtrI CACGTC 3 cut(s) 286, 604, 4079
BtsI GCAGTG 4 cut(s) 635, 1840, 1870, 3095
BveI ACCTGC 1 cut(s) 3634
CaiI CAGNNNCTG 2 cut(s) 1484, 2771
CciI TCATGA 1 cut(s) 3378
CfoI GCGC 7 cut(s) 192, 344, 359, 439, 469, 3096, 3444
Cfr10I RCCGGY 4 cut(s) 166, 377, 646, 4235
CseI GACGC 4 cut(s) 217, 251, 393, 555
Csp6I GTAC 6 cut(s) 289, 622, 1818, 3409, 3536, 4318
CspAI ACCGGT 1 cut(s) 377
CviQI GTAC 6 cut(s) 289, 622, 1818, 3409, 3536, 4318
DinI GGCGCC 1 cut(s) 3095
DpnI GATC 9 cut(s) 1417, 1552, 1741, 2916, 3136, 3147, 3270, 3546, 3879
DpnII GATC 9 cut(s) 1415, 1550, 1739, 2914, 3134, 3145, 3268, 3544, 3877
Eam1104I CTCTTC 3 cut(s) 149, 895, 4343
EarI CTCTTC 3 cut(s) 149, 895, 4343
EciI GGCGGA 4 cut(s) 34, 162, 171, 795
Ecl136II GAGCTC 2 cut(s) 430, 4336
Eco105I TACGTA 1 cut(s) 1301
Eco130I CCWWGG 5 cut(s) 2203, 2669, 2918, 3244, 4000
Eco147I AGGCCT 1 cut(s) 1728
Eco24I GRGCYC 5 cut(s) 432, 464, 2700, 3594, 4338
Eco31I GGTCTC 1 cut(s) 2370
Eco32I GATATC 2 cut(s) 1714, 2612
Eco47I GGWCC 6 cut(s) 1481, 1565, 1767, 2442, 2897, 4052
Eco53kI GAGCTC 2 cut(s) 430, 4336
Eco57I CTGAAG 5 cut(s) 789, 1272, 1674, 3461, 3684
Eco72I CACGTG 2 cut(s) 249, 3674
Eco81I CCTNAGG 1 cut(s) 3488
Eco88I CYCGRG 3 cut(s) 455, 596, 1385
EcoICRI GAGCTC 2 cut(s) 430, 4336
EcoNI CCTNNNNNAGG 2 cut(s) 3157, 3315
EcoO109I RGGNCCY 6 cut(s) 1293, 1481, 2150, 2442, 2768, 2897
EcoRI GAATTC 2 cut(s) 1330, 3125
EcoRV GATATC 2 cut(s) 1714, 2612
EcoT14I CCWWGG 5 cut(s) 2203, 2669, 2918, 3244, 4000
EcoT22I ATGCAT 1 cut(s) 2600
EcoT38I GRGCYC 5 cut(s) 432, 464, 2700, 3594, 4338
EgeI GGCGCC 1 cut(s) 3095
EheI GGCGCC 1 cut(s) 3095
ErhI CCWWGG 5 cut(s) 2203, 2669, 2918, 3244, 4000
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FaqI GGGAC 8 cut(s) 529, 561, 1012, 1124, 1614, 2116, 2455, 3377
FauI CCCGC 6 cut(s) 201, 481, 532, 552, 983, 3402
FauNDI CATATG 1 cut(s) 1936
FbaI TGATCA 1 cut(s) 3145
FblI GTMKAC 2 cut(s) 96, 4081
FriOI GRGCYC 5 cut(s) 432, 464, 2700, 3594, 4338
FspBI CTAG 7 cut(s) 72, 651, 1128, 1392, 2198, 3224, 3497
GlaI GCGC 7 cut(s) 191, 343, 358, 438, 468, 3095, 3443
GsaI CCCAGC 2 cut(s) 420, 999
HaeII RGCGCY 2 cut(s) 470, 3097
HapII CCGG 7 cut(s) 167, 324, 378, 647, 1759, 2881, 4236
HgaI GACGC 4 cut(s) 217, 251, 393, 555
HhaI GCGC 7 cut(s) 192, 344, 359, 439, 469, 3096, 3444
Hin1I GRCGYC 5 cut(s) 228, 243, 566, 3094, 3972
Hin6I GCGC 7 cut(s) 190, 342, 357, 437, 467, 3094, 3442
HinP1I GCGC 7 cut(s) 190, 342, 357, 437, 467, 3094, 3442
HincII GTYRAC 1 cut(s) 1053
HindII GTYRAC 1 cut(s) 1053
HindIII AAGCTT 6 cut(s) 590, 1204, 1604, 2418, 3803, 4254
HpaII CCGG 7 cut(s) 167, 324, 378, 647, 1759, 2881, 4236
HphI GGTGA 7 cut(s) 268, 884, 1748, 2099, 2219, 3263, 3332
Hpy99I CGWCG 5 cut(s) 202, 233, 287, 409, 481
Hsp92I GRCGYC 5 cut(s) 228, 243, 566, 3094, 3972
HspAI GCGC 7 cut(s) 190, 342, 357, 437, 467, 3094, 3442
KasI GGCGCC 1 cut(s) 3093
KpnI GGTACC 1 cut(s) 3412
KroI GCCGGC 2 cut(s) 166, 646
KroNI GCCGGC 2 cut(s) 168, 648
Ksp22I TGATCA 1 cut(s) 3145
Kzo9I GATC 9 cut(s) 1415, 1550, 1739, 2914, 3134, 3145, 3268, 3544, 3877
LguI GCTCTTC 1 cut(s) 4343
LmnI GCTCC 7 cut(s) 175, 660, 2584, 2703, 2779, 3032, 3650
LweI GCATC 7 cut(s) 607, 825, 1000, 1811, 2358, 3592, 3775
MaeI CTAG 7 cut(s) 72, 651, 1128, 1392, 2198, 3224, 3497
MaeIII GTNAC 9 cut(s) 380, 633, 1190, 1594, 2407, 2862, 3367, 3469, 4139
MalI GATC 9 cut(s) 1417, 1552, 1741, 2916, 3136, 3147, 3270, 3546, 3879
MboI GATC 9 cut(s) 1415, 1550, 1739, 2914, 3134, 3145, 3268, 3544, 3877
MfeI CAATTG 2 cut(s) 2250, 4276
MflI RGATCY 4 cut(s) 1550, 1739, 2914, 3877
MhlI GDGCHC 9 cut(s) 212, 432, 464, 2503, 2700, 2724, 2880, 3594, 4338
MluI ACGCGT 1 cut(s) 402
Mly113I GGCGCC 1 cut(s) 3094
MlyI GAGTC 7 cut(s) 511, 1162, 1265, 2831, 2870, 2923, 3391
MmeI TCCRAC 2 cut(s) 3616, 3847
Mph1103I ATGCAT 1 cut(s) 2600
MroNI GCCGGC 2 cut(s) 166, 646
MroXI GAANNNNTTC 2 cut(s) 1068, 3129
MslI CAYNNNNRTG 4 cut(s) 1434, 3019, 3079, 3738
MspA1I CMGCKG 4 cut(s) 339, 1994, 2081, 3949
MspCI CTTAAG 1 cut(s) 3157
MspI CCGG 7 cut(s) 167, 324, 378, 647, 1759, 2881, 4236
MunI CAATTG 2 cut(s) 2250, 4276
Mva1269I GAATGC 6 cut(s) 35, 1582, 2485, 3045, 3436, 4005
MvnI CGCG 5 cut(s) 190, 344, 359, 404, 3175
NaeI GCCGGC 2 cut(s) 168, 648
NarI GGCGCC 1 cut(s) 3094
NciI CCSGG 2 cut(s) 324, 1760
NdeI CATATG 1 cut(s) 1936
NdeII GATC 9 cut(s) 1415, 1550, 1739, 2914, 3134, 3145, 3268, 3544, 3877
NgoMIV GCCGGC 2 cut(s) 166, 646
NheI GCTAGC 2 cut(s) 1391, 3223
NmeAIII GCCGAG 3 cut(s) 443, 689, 1727
NmuCI GTSAC 5 cut(s) 633, 1190, 1594, 2407, 2862
NsiI ATGCAT 1 cut(s) 2600
NspI RCATGY 6 cut(s) 1902, 2460, 2620, 3771, 4088, 4286
NspV TTCGAA 1 cut(s) 269
OliI CACNNNNGTG 1 cut(s) 3738
PaeR7I CTCGAG 2 cut(s) 455, 1385
PagI TCATGA 1 cut(s) 3378
PceI AGGCCT 1 cut(s) 1728
PciSI GCTCTTC 1 cut(s) 4343
PctI GAATGC 6 cut(s) 35, 1582, 2485, 3045, 3436, 4005
PdiI GCCGGC 2 cut(s) 168, 648
PdmI GAANNNNTTC 2 cut(s) 1068, 3129
PfoI TCCNGGA 1 cut(s) 2817
PinAI ACCGGT 1 cut(s) 377
PleI GAGTC 7 cut(s) 511, 1161, 1265, 2830, 2869, 2923, 3390
PluTI GGCGCC 1 cut(s) 3097
PmaCI CACGTG 2 cut(s) 249, 3674
PmlI CACGTG 2 cut(s) 249, 3674
PpsI GAGTC 7 cut(s) 511, 1161, 1265, 2830, 2869, 2923, 3390
Ppu21I YACGTR 5 cut(s) 249, 1301, 2735, 3539, 3674
PpuMI RGGWCCY 3 cut(s) 1481, 2442, 2897
PshBI ATTAAT 2 cut(s) 3000, 4113
PsiI TTATAA 4 cut(s) 834, 1905, 2475, 4152
Psp124BI GAGCTC 2 cut(s) 432, 4338
Psp5II RGGWCCY 3 cut(s) 1481, 2442, 2897
PspCI CACGTG 2 cut(s) 249, 3674
PspFI CCCAGC 2 cut(s) 416, 995
PspPPI RGGWCCY 3 cut(s) 1481, 2442, 2897
PspXI VCTCGAGB 1 cut(s) 1385
PstNI CAGNNNCTG 2 cut(s) 1484, 2771
PsuI RGATCY 4 cut(s) 1550, 1739, 2914, 3877
PvuII CAGCTG 4 cut(s) 339, 1994, 2081, 3949
RsaI GTAC 6 cut(s) 290, 623, 1819, 3410, 3537, 4319
RsaNI GTAC 6 cut(s) 289, 622, 1818, 3409, 3536, 4318
RseI CAYNNNNRTG 4 cut(s) 1434, 3019, 3079, 3738
SacI GAGCTC 2 cut(s) 432, 4338
SapI GCTCTTC 1 cut(s) 4343
Sau3AI GATC 9 cut(s) 1415, 1550, 1739, 2914, 3134, 3145, 3268, 3544, 3877
SchI GAGTC 7 cut(s) 511, 1162, 1265, 2831, 2870, 2923, 3391
SduI GDGCHC 9 cut(s) 212, 432, 464, 2503, 2700, 2724, 2880, 3594, 4338
SfaNI GCATC 7 cut(s) 607, 825, 1000, 1811, 2358, 3592, 3775
SfcI CTRYAG 2 cut(s) 919, 1528
SfoI GGCGCC 1 cut(s) 3095
Sfr274I CTCGAG 2 cut(s) 455, 1385
SfuI TTCGAA 1 cut(s) 269
SinI GGWCC 6 cut(s) 1481, 1565, 1767, 2442, 2897, 4052
SlaI CTCGAG 2 cut(s) 455, 1385
SmiMI CAYNNNNRTG 4 cut(s) 1434, 3019, 3079, 3738
SmlI CTYRAG 4 cut(s) 455, 1385, 3157, 3806
SmoI CTYRAG 4 cut(s) 455, 1385, 3157, 3806
SnaBI TACGTA 1 cut(s) 1301
SseBI AGGCCT 1 cut(s) 1728
SspDI GGCGCC 1 cut(s) 3093
SspMI CTAG 7 cut(s) 72, 651, 1128, 1392, 2198, 3224, 3497
SstI GAGCTC 2 cut(s) 432, 4338
StuI AGGCCT 1 cut(s) 1728
StyI CCWWGG 5 cut(s) 2203, 2669, 2918, 3244, 4000
TaqI TCGA 8 cut(s) 269, 426, 456, 674, 1021, 1386, 2991, 3133
TatI WGTACW 3 cut(s) 288, 1817, 4317
TauI GCSGC 6 cut(s) 166, 187, 190, 499, 502, 3463
TseFI GTSAC 5 cut(s) 633, 1190, 1594, 2407, 2862
Tsp45I GTSAC 5 cut(s) 633, 1190, 1594, 2407, 2862
TspGWI ACGGA 2 cut(s) 82, 2358
Vha464I CTTAAG 1 cut(s) 3157
VneI GTGCAC 2 cut(s) 208, 2876
VpaK11BI GGWCC 6 cut(s) 1481, 1565, 1767, 2442, 2897, 4052
VspI ATTAAT 2 cut(s) 3000, 4113
XagI CCTNNNNNAGG 2 cut(s) 3157, 3315
XapI RAATTY 6 cut(s) 737, 942, 1235, 1330, 3125, 3745
XceI RCATGY 6 cut(s) 1902, 2460, 2620, 3771, 4088, 4286
XcmI CCANNNNNNNNNTGG 3 cut(s) 2335, 2387, 3881
XhoI CTCGAG 2 cut(s) 455, 1385
XmiI GTMKAC 2 cut(s) 96, 4081
XmnI GAANNNNTTC 2 cut(s) 1068, 3129
XspI CTAG 7 cut(s) 72, 651, 1128, 1392, 2198, 3224, 3497
ZraI GACGTC 1 cut(s) 3973
Zsp2I ATGCAT 1 cut(s) 2600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.