MD08G1108700.v1.1

glycine-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002500
Physical Location & Seq
Forward (+)
9567650 .. 9577771
10122 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1108700.v1.1.491

Sequence Viewer

Length: 4341 bp
ATGGCTCGCTTCCGCACTCCCACTCTCCTCCTCGCATTCCTCTCCGCCCTTGTTGCCCTAACCGCAAACCCTAGCCACCTCCTCCTCGCGTCCGAAGATGATGACTTTCCGATTAACGATTCCGATTCCTATCTCTTCCACCAAGACTACTCGCCGCCGGCTCCGCCACCTCCTCCGCCGCTCCCGCCGTCGGTCTCTTGCACCGATGACCTCGGTGGCGTCGGCTCTCTGGACGCCACGTGTCAGATAGTCTCCGATTCGAACCTCACCAGCGACGTGTACATAACAGGGAAGGGCAACTTTTATATCCTGCCCGGGGTGAGATTCGGCTGCGCGATTCCTGGCTGCGCGATAATTATTAACATTACTGGTAACTTTAGCTTAGGCAGCAACGCGTCGCTTTTGGCCGGGGCTTTCGAGCTCACGGCGTGCAATGCCAGCTTCCTCAACGGCTCGGCGCTGAACACGACGGCTTTGGCTGGGAAGCCGCCTCCGCAGACAAGTGGGACTCCGCAGGGGATAGATGGGGCGGGTGGGGGACACGGAGGGCGTGGGGCGTGTTGTTTGGTGGACAAGACGAAGCTTCCGGAGGACGTCTGGGGTGGGGATGCATACTCGTGGTCGACGCTGCAGAGGCCGGCTAGTTTTGGGAGCCGAGGCGGGTCGACGAGTAAGGAGGTGGATTATGGAGGTTTAGGTGGAGGGAGGGTGAGGCTCCAAGTGAAGGAATTGCTGGTGGTGGAGGGAAGTGTTTTGGCTGAAGGCGGTGGCGGAGGGAATAGAGGTGGCGGTGGTTCTGGTGGCAGTATCTACATTAAGGCTCATAAAATGACTGGTAGTGGCAGGATAAGTGCCTGTGGTGGTGATGGTTACGCTGGTGGTGGTGGTGGAAGAGTGTCAGTTGATGTCTATAGCAGGCATGATGACCCCAAAATTTTTGTGCATGGAGGAAATAGCTATTCTTGTCCAGAAAATGCAGGCGGGGCTGGGACTTTATATGATGCAGTTCCTCGAAGCCTTATTGTTAGCAATCATAACAAGTCAACAGATACAGAATCACTTCTTATGGAATTTCCTTATCAGCCTCTTTGGACAAATGTTTATATTCAAAATAAGGCGAGGGCGACTGTCCCATTGCTTTGGAGTCGTGTCCAGGTGCAAGGACAGATAAGCCTTTTGAGTGATGGTGTGCTAAGCTTTGGACTTCAACATTATGCTTCATCAGAGTTTGAGTTATTGGCTGAAGAATTGTTGATGAGCGACTCTGTAATCAAAGTGTATGGGGCCTTACGCATGACTGTAAAAATGTTCTTGATGTGGAATTCCAAGATGCTCATTGATGGTGGAGGGGAAGAAGCTGTTGAGACATCCTTACTCGAGTCTAGCAACTTGGTGGTTCTCAGGGGATCATCTGTGATACACTCTAATGCAAATCTGGGAGTTCATGGACAAGGTTTATTGAACTTATCAGGACCTGGAGATTGGATTCAAGCACAACGTCTGGTTCTATCGCTATTCTACAGTATTCATGTTGGACCGGGATCTGTTTTGCGTGGTCCTTTAGAGAATGCCGCATCTGATTCTGTAACACCAAAGCTTTATTGTGAAAACAAAGATTGCCCCTATGAGCTACTTCTTCCACCTGAAGATTGCAATGTGAACTCATCCCTGCCCTTTACTCTTCAGGTATGCCGAGTTGAGGATATCATTATTGAAGGCCTCATAAAAGGATCTGTTGTTAATTTTCACCGGGCAAGGACCATTGCTATCCATTCTTCTGGAGAAATAAGTGCATCAGGAATGGGTTGTACTGGTGGTATTGGCAGTGGCAACATTTTAAGCAATGGAATTAGCAGTGGTGGCGGGCATGGAGGTAAAGGTGGGGTGGCATGTTATAATGGCAACTGTATTGAGGGTGGAATCTCATATGGGAATGCAAAGTTGCCTTGCGAACTTGGTAGTGGAAGTGGATATGACCTTTCAGCTGGCTTAACTGCTGGCGGTGGTATTATCATAATGGGTTCTTCAGAACATCCCTTGTCAAGTTTGTCTGTTGAAGGCGCGATGACAGCTGATGGTGAAAGTTTTGAAGGGACTGTTGTGGAGGAAAAGTATGCCCTTGTTGATAACACAACTGGAGGGCCTGGGGGTGGGTCTGGTGGAACTATACTTCTGTTCTTGCGAACACTAGCTCTTGGTGAGACTGCTATTCTTTCAAGTGTTGGGGGATATGGTAGTTCCATCGGTGGTGGTGGAGGTGGTGGTGGAAGGATTCATTTTCATTGGTCAGATATTCCCACTGGAGATGTGTATCAGCCCATTGCAAGTGTGGACGGAAGCATCCTTGCAGGGGGAGGGGTGGGTAGAGACCAGGGTGGTGCTGGAGAAAATGGAACATTGACAGGTGCAGATTGCCCAAAAGGTCTCTATGGGACCTTTTGTGAGGCATGTCCAGCTGGTACTTACAAGAATGCTATTGGGTCTGATAGGGCACTCTGTCATCATTGTCCTGCTACTCAGCTTCCCCCTCGTGCGATTTATATTCCTGTCCGAGGTGGTGTTGCTGAGATTCCTTGTCCTTACAAATGCATTTCAGACAGATATCACATGCCAAACTGTTTTACAGCTCTTGAAGAATTGATTTACACATTTGGTGGGCCTTGGCTATTCGGTCTTCTTCTGATTGGGCTCCTCATCCTGTTGGCTTTGGTACTCAGTGTTGCACGTATGAAATTGGTTGGGGTTGATGAATTACCAGGCCCTGCTCCCACTCAACATGGCTCACAAATAGACCACTCTTTCCCTTTCCTAGAGTCATTGAATGAGGTTTTAGAAACAAACAGGGCTGAGGAGTCACAGAGCCATGTGCACCGGATGTATTTTATGGGTCCTAATACTTTTGGTGATCCTTGGCATTTGCCCCACACACCTCCAGAACAAATAAAAGAGATTGTATATGAGGGGCCATTCAATACATTTGTCGATGAGATTAATTCCATAGCCACTTATCAGTGGTGGGAGGGAGCAATGTACATCATTCTTTCTGTTCTTGCATACCCGCTTGCATGGTCATGGCAGCAGTGCCGCCGAAGATTGAAATTGCAACATCTACGTGAATTTGTTCGATCGGAGTATGATCATGCCTGCTTACGGTCCTGCCGTTCGCGTGCTTTGTATGAAGGGATTAAGGTAGCTGCAACTTCTGATTTAATGCTAGCATATATCGACTTCTTCCTTGGTGGTGATGAAAAGAGAACTGATCTTCCCCCCCGTTTACATCAAAGATTTCCTATTTCATTACCCTTTGGAGGTGATGGAAGTTACATGGCCCCTTTCTCACTTCACAGTGATAACATTCTTACAAGCCTCATGAGTCAGTCAGTCCCGCCTACCGCATGGTACCGTATGGTTGCTGGTTTGAATGCACAGTTACGCTTAGTTTGCCGTGGACGGCTAAGAGTTACACTTCAGCCTGTCCTTCGGTGGCTAGAACATTATGCCAATCCTGCCTTGAAGATTTATGGTGTACGTGTTGATCTTGCCTGGTTTCAGGCTACATCTTTTGGTTATTGTCATTATGGGCTCGTGGTGGATGTTCTTGAAGAAGACAGTGAACCTGCCTCTGTTAGGAACATTGATGGAGAAATACGAACTGAAGAATCACGTGCAAACTACAAAGAAGATTCATCTGGTCATTTGAGAGAATCGCTCCTAAACCAGTCTCGCAGAAGTGAAAATTTTATGAGGCCAAAAAGAGAATACGGAGGGATTATAGATGCTAACAACTTACAAACGCTTGAAGAAAAGAGAGACATGTTTTATCTTCTCTCTTTTATACTTCATAATACTAAACCTGTTGGACACCAGGATCTTGTTGGTTTGGTCATCTCAATGCTTCTTTTAGGAGATTTGAGCTTAGTTTTGCTTACATTACTCCAGCTGTATTCGATTTCATTGGTGGACGTCTTTCTGGTTCTATTTATTTTACCCCTTGGCATACTTCTCCCGTTTCCTGCTGGTATCAATGCTCTATTCAGTCATGGACCTAGACGCTCTGCTGGGCTTGCACGTCTTTATGCTTTGTGGAACCTTACATCCTTGTTTAATGTTGCGGTTGCTTTTGGTTGCGGTTACGTTCATTATAGCACTCAATCATCAAGTAAAAAGCATCCCTTTCAACCGTGGAGTAACATGGACGAAAGTGAATGGTGGATTTTTCCCGCCGGTCTGCTGCTTTGTAAAGTTTTTCAATCCCAGCTAGTCAATTGGCACGTTGCGAATCTGGAGATTCAGGACCGTTCGTTGTACAGCAATGATTTTGAGCTCTTCTGGCAGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1447

Amino Acids

155.0

Weight (kDa)

5.54

Isoelectric Point (pI)

49.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8003 PF26010 801 - 874 1.6e-34 Domain of unknown function (DUF8003)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1896
AatII GACGTC 2 cut(s) 597, 3966
Acc36I ACCTGC 1 cut(s) 3625
Acc65I GGTACC 1 cut(s) 3399
AccB1I GGYRCC 1 cut(s) 3399
AccBSI CCGCTC 1 cut(s) 181
AccI GTMKAC 2 cut(s) 623, 665
AccII CGCG 6 cut(s) 89, 335, 350, 395, 2063, 3166
AccIII TCCGGA 1 cut(s) 586
AclWI GGATC 5 cut(s) 1414, 1549, 1738, 2900, 3876
AcoI YGGCCR 1 cut(s) 405
AcsI RAATTY 5 cut(s) 933, 1070, 1321, 3116, 3736
AcuI CTGAAG 7 cut(s) 780, 1263, 1665, 1667, 2010, 3452, 3675
AcvI CACGTG 2 cut(s) 240, 3665
AcyI GRCGYC 4 cut(s) 219, 234, 594, 3963
AdeI CACNNNGTG 1 cut(s) 429
AfaI GTAC 8 cut(s) 281, 1810, 2461, 2712, 3032, 3401, 3528, 4307
AflIII ACRYGT 5 cut(s) 239, 276, 393, 3529, 3813
AjiI CACGTC 2 cut(s) 277, 4070
AjnI CCWGG 8 cut(s) 340, 1152, 1474, 2143, 2370, 2755, 3542, 3864
AloI GAACNNNNNNTCC 4 cut(s) 3247, 3279, 4079, 4111
Alw21I GWGCWC 3 cut(s) 423, 2871, 4326
Alw26I GTCTC 8 cut(s) 199, 256, 1358, 2195, 2361, 2429, 3726, 3804
Alw44I GTGCAC 1 cut(s) 2867
AlwI GGATC 5 cut(s) 1414, 1549, 1738, 2900, 3876
AlwNI CAGNNNCTG 2 cut(s) 1475, 2762
Ama87I CYCGRG 2 cut(s) 314, 1376
Aor13HI TCCGGA 1 cut(s) 586
ApaLI GTGCAC 1 cut(s) 2867
ApeKI GCWGC 7 cut(s) 330, 345, 387, 628, 3076, 3194, 4231
ApoI RAATTY 5 cut(s) 933, 1070, 1321, 3116, 3736
AseI ATTAAT 1 cut(s) 2991
Asp700I GAANNNNTTC 2 cut(s) 1059, 3120
Asp718I GGTACC 1 cut(s) 3399
AspLEI GCGC 4 cut(s) 335, 350, 460, 2063
AsuC2I CCSGG 5 cut(s) 315, 316, 409, 1539, 1751
AsuII TTCGAA 1 cut(s) 260
AsuNHI GCTAGC 1 cut(s) 3214
AvaI CYCGRG 2 cut(s) 314, 1376
AvaII GGWCC 9 cut(s) 1472, 1535, 1556, 1758, 2433, 2888, 3153, 4043, 4294
BaeGI GKGCMC 2 cut(s) 2494, 2871
BanI GGYRCC 1 cut(s) 3399
BanII GRGCYC 4 cut(s) 423, 2691, 3585, 4326
BauI CACGAG 3 cut(s) 616, 2529, 3584
BbrPI CACGTG 2 cut(s) 240, 3665
BbsI GAAGAC 2 cut(s) 2666, 3612
Bbv12I GWGCWC 3 cut(s) 423, 2871, 4326
BbvCI CCTCAGC 1 cut(s) 2847
BbvI GCAGC 7 cut(s) 317, 332, 399, 615, 3088, 3181, 4218
BccI CCATC 8 cut(s) 518, 860, 1178, 1334, 2069, 2249, 3308, 3632
BceAI ACGGC 7 cut(s) 172, 441, 466, 486, 3144, 3429, 3467
BciT130I CCWGG 8 cut(s) 342, 1154, 1476, 2145, 2372, 2757, 3544, 3866
BclI TGATCA 1 cut(s) 3136
BcnI CCSGG 5 cut(s) 315, 316, 409, 1539, 1751
BcoDI GTCTC 8 cut(s) 199, 256, 1358, 2195, 2361, 2429, 3726, 3804
BfaI CTAG 9 cut(s) 72, 642, 1383, 2189, 2810, 3215, 3488, 4047, 4259
BfmI CTRYAG 3 cut(s) 629, 910, 1519
BfoI RGCGCY 1 cut(s) 461
BfuAI ACCTGC 1 cut(s) 3625
BlpI GCTNAGC 1 cut(s) 1193
Bme18I GGWCC 9 cut(s) 1472, 1535, 1556, 1758, 2433, 2888, 3153, 4043, 4294
BmeT110I CYCGRG 2 cut(s) 314, 1376
BmgBI CACGTC 2 cut(s) 277, 4070
BmsI GCATC 8 cut(s) 598, 991, 1320, 1583, 1802, 2349, 3766, 4177
BmtI GCTAGC 1 cut(s) 3218
BpiI GAAGAC 2 cut(s) 2666, 3612
BplI GAGNNNNNCTC 2 cut(s) 3693, 3725
BpmI CTGGAG 8 cut(s) 1497, 1800, 2157, 2322, 2403, 2916, 3920, 4304
Bpu10I CCTNAGC 2 cut(s) 382, 2847
Bpu1102I GCTNAGC 1 cut(s) 1193
Bpu14I TTCGAA 1 cut(s) 260
BpuMI CCSGG 5 cut(s) 315, 316, 409, 1539, 1751
BsaAI YACGTR 5 cut(s) 240, 2726, 3113, 3530, 3665
BsaBI GATNNNNATC 2 cut(s) 129, 2310
BsaHI GRCGYC 4 cut(s) 219, 234, 594, 3963
BsaI GGTCTC 3 cut(s) 199, 2361, 2429
BsaWI WCCGGW 2 cut(s) 586, 2871
BsaXI ACNNNNNCTCC 4 cut(s) 693, 723, 2096, 2126
Bse118I RCCGGY 3 cut(s) 157, 637, 4223
Bse1I ACTGG 6 cut(s) 373, 838, 1816, 2140, 2305, 3718
Bse3DI GCAATG 8 cut(s) 439, 1133, 1660, 1761, 1849, 2319, 3033, 4318
Bse8I GATNNNNATC 2 cut(s) 129, 2310
BseAI TCCGGA 1 cut(s) 586
BseBI CCWGG 8 cut(s) 342, 1154, 1476, 2145, 2372, 2757, 3544, 3866
BseJI GATNNNNATC 2 cut(s) 129, 2310
BseMI GCAATG 8 cut(s) 439, 1133, 1660, 1761, 1849, 2319, 3033, 4318
BseMII CTCAG 5 cut(s) 1414, 2531, 2556, 2728, 2838
BseNI ACTGG 6 cut(s) 373, 838, 1816, 2140, 2305, 3718
BseRI GAGGAG 7 cut(s) 17, 20, 71, 74, 162, 2681, 2864
BseSI GKGCMC 2 cut(s) 2494, 2871
BseXI GCAGC 7 cut(s) 317, 332, 399, 615, 3088, 3181, 4218
BseYI CCCAGC 4 cut(s) 479, 986, 4058, 4254
BsgI GTGCAG 1 cut(s) 2427
Bsh1236I CGCG 6 cut(s) 89, 335, 350, 395, 2063, 3166
Bsh1285I CGRYCG 1 cut(s) 3128
BshNI GGYRCC 1 cut(s) 3399
BsiEI CGRYCG 1 cut(s) 3128
BsiHKAI GWGCWC 3 cut(s) 423, 2871, 4326
BsiHKCI CYCGRG 2 cut(s) 314, 1376
BsiSI CCGG 9 cut(s) 158, 315, 408, 587, 638, 1538, 1750, 2872, 4224
BslFI GGGAC 7 cut(s) 520, 552, 1003, 1115, 2107, 2446, 3368
BsmAI GTCTC 8 cut(s) 199, 256, 1358, 2195, 2361, 2429, 3726, 3804
BsmFI GGGAC 7 cut(s) 520, 552, 1003, 1115, 2107, 2446, 3368
BsmI GAATGC 5 cut(s) 35, 1573, 1939, 2476, 3427
Bso31I GGTCTC 3 cut(s) 199, 2361, 2429
BsoBI CYCGRG 2 cut(s) 314, 1376
Bsp119I TTCGAA 1 cut(s) 260
Bsp1286I GDGCHC 6 cut(s) 423, 2494, 2691, 2871, 3585, 4326
Bsp13I TCCGGA 1 cut(s) 586
Bsp1407I TGTACA 3 cut(s) 279, 3030, 4305
Bsp143I GATC 9 cut(s) 1406, 1541, 1730, 2905, 3125, 3136, 3259, 3535, 3868
Bsp1720I GCTNAGC 1 cut(s) 1193
BspCNI CTCAG 5 cut(s) 1413, 2530, 2557, 2727, 2839
BspEI TCCGGA 1 cut(s) 586
BspFNI CGCG 6 cut(s) 89, 335, 350, 395, 2063, 3166
BspHI TCATGA 1 cut(s) 3369
BspMAI CTGCAG 1 cut(s) 633
BspMI ACCTGC 1 cut(s) 3625
BspOI GCTAGC 1 cut(s) 3218
BspPI GGATC 5 cut(s) 1414, 1549, 1738, 2900, 3876
BspQI GCTCTTC 1 cut(s) 4331
BspT104I TTCGAA 1 cut(s) 260
BspT107I GGYRCC 1 cut(s) 3399
BspTNI GGTCTC 3 cut(s) 199, 2361, 2429
BsrBI CCGCTC 1 cut(s) 181
BsrDI GCAATG 8 cut(s) 439, 1133, 1660, 1761, 1849, 2319, 3033, 4318
BsrFI RCCGGY 3 cut(s) 157, 637, 4223
BsrGI TGTACA 3 cut(s) 279, 3030, 4305
BsrI ACTGG 6 cut(s) 373, 838, 1816, 2140, 2305, 3718
BssAI RCCGGY 3 cut(s) 157, 637, 4223
BssMI GATC 9 cut(s) 1406, 1541, 1730, 2905, 3125, 3136, 3259, 3535, 3868
BssNI GRCGYC 4 cut(s) 219, 234, 594, 3963
BssSI CACGAG 3 cut(s) 616, 2529, 3584
BssT1I CCWWGG 4 cut(s) 2660, 2909, 3235, 3991
Bst2BI CACGAG 3 cut(s) 616, 2529, 3584
Bst2UI CCWGG 8 cut(s) 342, 1154, 1476, 2145, 2372, 2757, 3544, 3866
Bst6I CTCTTC 4 cut(s) 140, 886, 1686, 4331
BstACI GRCGYC 4 cut(s) 219, 234, 594, 3963
BstAUI TGTACA 3 cut(s) 279, 3030, 4305
BstBAI YACGTR 5 cut(s) 240, 2726, 3113, 3530, 3665
BstBI TTCGAA 1 cut(s) 260
BstDSI CCRYGG 2 cut(s) 3445, 4181
BstENI CCTNNNNNAGG 2 cut(s) 2550, 3625
BstFNI CGCG 6 cut(s) 89, 335, 350, 395, 2063, 3166
BstH2I RGCGCY 1 cut(s) 461
BstHHI GCGC 4 cut(s) 335, 350, 460, 2063
BstKTI GATC 9 cut(s) 1409, 1544, 1733, 2908, 3128, 3139, 3262, 3538, 3871
BstMAI GTCTC 8 cut(s) 199, 256, 1358, 2195, 2361, 2429, 3726, 3804
BstMBI GATC 9 cut(s) 1406, 1541, 1730, 2905, 3125, 3136, 3259, 3535, 3868
BstMCI CGRYCG 1 cut(s) 3128
BstNI CCWGG 8 cut(s) 342, 1154, 1476, 2145, 2372, 2757, 3544, 3866
BstNSI RCATGY 4 cut(s) 1893, 2451, 2611, 3817
BstSFI CTRYAG 3 cut(s) 629, 910, 1519
BstSLI GKGCMC 2 cut(s) 2494, 2871
BstUI CGCG 6 cut(s) 89, 335, 350, 395, 2063, 3166
BstV1I GCAGC 7 cut(s) 317, 332, 399, 615, 3088, 3181, 4218
BstV2I GAAGAC 2 cut(s) 2666, 3612
BstX2I RGATCY 3 cut(s) 1541, 1730, 3868
BstXI CCANNNNNNTGG 2 cut(s) 1140, 1778
BstYI RGATCY 3 cut(s) 1541, 1730, 3868
BtgI CCRYGG 2 cut(s) 3445, 4181
BtgZI GCGATG 1 cut(s) 2078
BtrI CACGTC 2 cut(s) 277, 4070
BtsI GCAGTG 3 cut(s) 1831, 1861, 3086
BtsIMutI CAGTG 8 cut(s) 1831, 1861, 2298, 2722, 3017, 3086, 3352, 3616
BveI ACCTGC 1 cut(s) 3625
CaiI CAGNNNCTG 2 cut(s) 1475, 2762
CciI TCATGA 1 cut(s) 3369
CfoI GCGC 4 cut(s) 335, 350, 460, 2063
Cfr10I RCCGGY 3 cut(s) 157, 637, 4223
Cfr9I CCCGGG 1 cut(s) 314
CseI GACGC 6 cut(s) 78, 208, 242, 384, 634, 4059
Csp6I GTAC 8 cut(s) 280, 1809, 2460, 2711, 3031, 3400, 3527, 4306
CviQI GTAC 8 cut(s) 280, 1809, 2460, 2711, 3031, 3400, 3527, 4306
DpnI GATC 9 cut(s) 1408, 1543, 1732, 2907, 3127, 3138, 3261, 3537, 3870
DpnII GATC 9 cut(s) 1406, 1541, 1730, 2905, 3125, 3136, 3259, 3535, 3868
DraIII CACNNNGTG 1 cut(s) 429
EaeI YGGCCR 1 cut(s) 405
Eam1104I CTCTTC 4 cut(s) 140, 886, 1686, 4331
EarI CTCTTC 4 cut(s) 140, 886, 1686, 4331
EciI GGCGGA 4 cut(s) 34, 153, 165, 786
Ecl136II GAGCTC 2 cut(s) 421, 4324
Eco130I CCWWGG 4 cut(s) 2660, 2909, 3235, 3991
Eco147I AGGCCT 1 cut(s) 1719
Eco24I GRGCYC 4 cut(s) 423, 2691, 3585, 4326
Eco31I GGTCTC 3 cut(s) 199, 2361, 2429
Eco32I GATATC 2 cut(s) 1705, 2603
Eco47I GGWCC 9 cut(s) 1472, 1535, 1556, 1758, 2433, 2888, 3153, 4043, 4294
Eco53kI GAGCTC 2 cut(s) 421, 4324
Eco57I CTGAAG 7 cut(s) 780, 1263, 1665, 1667, 2010, 3452, 3675
Eco72I CACGTG 2 cut(s) 240, 3665
Eco88I CYCGRG 2 cut(s) 314, 1376
EcoICRI GAGCTC 2 cut(s) 421, 4324
EcoNI CCTNNNNNAGG 2 cut(s) 2550, 3625
EcoO109I RGGNCCY 6 cut(s) 1284, 1472, 2141, 2433, 2759, 2888
EcoRI GAATTC 1 cut(s) 1321
EcoRII CCWGG 8 cut(s) 340, 1152, 1474, 2143, 2370, 2755, 3542, 3864
EcoRV GATATC 2 cut(s) 1705, 2603
EcoT14I CCWWGG 4 cut(s) 2660, 2909, 3235, 3991
EcoT22I ATGCAT 2 cut(s) 613, 2591
EcoT38I GRGCYC 4 cut(s) 423, 2691, 3585, 4326
ErhI CCWWGG 4 cut(s) 2660, 2909, 3235, 3991
FalI AAGNNNNNCTT 4 cut(s) 284, 316, 4157, 4189
FaqI GGGAC 7 cut(s) 520, 552, 1003, 1115, 2107, 2446, 3368
FauI CCCGC 8 cut(s) 192, 523, 653, 974, 1856, 3066, 3393, 4228
FauNDI CATATG 1 cut(s) 1927
FbaI TGATCA 1 cut(s) 3136
FblI GTMKAC 2 cut(s) 623, 665
FriOI GRGCYC 4 cut(s) 423, 2691, 3585, 4326
FspBI CTAG 9 cut(s) 72, 642, 1383, 2189, 2810, 3215, 3488, 4047, 4259
GlaI GCGC 4 cut(s) 334, 349, 459, 2062
GsaI CCCAGC 4 cut(s) 483, 990, 4062, 4258
GsuI CTGGAG 8 cut(s) 1497, 1800, 2157, 2322, 2403, 2916, 3920, 4304
HaeII RGCGCY 1 cut(s) 461
HapII CCGG 9 cut(s) 158, 315, 408, 587, 638, 1538, 1750, 2872, 4224
HgaI GACGC 6 cut(s) 78, 208, 242, 384, 634, 4059
HhaI GCGC 4 cut(s) 335, 350, 460, 2063
Hin1I GRCGYC 4 cut(s) 219, 234, 594, 3963
Hin6I GCGC 4 cut(s) 333, 348, 458, 2061
HinP1I GCGC 4 cut(s) 333, 348, 458, 2061
HincII GTYRAC 3 cut(s) 624, 666, 1044
HindII GTYRAC 3 cut(s) 624, 666, 1044
HindIII AAGCTT 3 cut(s) 581, 1195, 1595
HpaII CCGG 9 cut(s) 158, 315, 408, 587, 638, 1538, 1750, 2872, 4224
Hpy99I CGWCG 7 cut(s) 193, 224, 278, 400, 472, 628, 670
HpyAV CCTTC 9 cut(s) 286, 718, 755, 1709, 2051, 2084, 2262, 3173, 3488
Hsp92I GRCGYC 4 cut(s) 219, 234, 594, 3963
HspAI GCGC 4 cut(s) 333, 348, 458, 2061
Kpn2I TCCGGA 1 cut(s) 586
KpnI GGTACC 1 cut(s) 3403
KroI GCCGGC 2 cut(s) 157, 637
KroNI GCCGGC 2 cut(s) 159, 639
Ksp22I TGATCA 1 cut(s) 3136
Kzo9I GATC 9 cut(s) 1406, 1541, 1730, 2905, 3125, 3136, 3259, 3535, 3868
LguI GCTCTTC 1 cut(s) 4331
LmnI GCTCC 8 cut(s) 166, 186, 651, 720, 2694, 2770, 3023, 3714
Lsp1109I GCAGC 7 cut(s) 317, 332, 399, 615, 3088, 3181, 4218
LweI GCATC 8 cut(s) 598, 991, 1320, 1583, 1802, 2349, 3766, 4177
MaeI CTAG 9 cut(s) 72, 642, 1383, 2189, 2810, 3215, 3488, 4047, 4259
MaeIII GTNAC 9 cut(s) 371, 869, 1585, 2853, 3320, 3429, 3460, 4130, 4187
MalI GATC 9 cut(s) 1408, 1543, 1732, 2907, 3127, 3138, 3261, 3537, 3870
MbiI CCGCTC 1 cut(s) 181
MboI GATC 9 cut(s) 1406, 1541, 1730, 2905, 3125, 3136, 3259, 3535, 3868
MfeI CAATTG 1 cut(s) 4264
MflI RGATCY 3 cut(s) 1541, 1730, 3868
MhlI GDGCHC 6 cut(s) 423, 2494, 2691, 2871, 3585, 4326
MluI ACGCGT 1 cut(s) 393
MlyI GAGTC 7 cut(s) 502, 1153, 1256, 1388, 2822, 2861, 3382
MmeI TCCRAC 2 cut(s) 1513, 3838
Mph1103I ATGCAT 2 cut(s) 613, 2591
MroI TCCGGA 1 cut(s) 586
MroNI GCCGGC 2 cut(s) 157, 637
MroXI GAANNNNTTC 2 cut(s) 1059, 3120
MslI CAYNNNNRTG 5 cut(s) 616, 1425, 3070, 3111, 3890
MspA1I CMGCKG 4 cut(s) 1985, 2072, 2456, 3940
MspI CCGG 9 cut(s) 158, 315, 408, 587, 638, 1538, 1750, 2872, 4224
MunI CAATTG 1 cut(s) 4264
Mva1269I GAATGC 5 cut(s) 35, 1573, 1939, 2476, 3427
MvaI CCWGG 8 cut(s) 342, 1154, 1476, 2145, 2372, 2757, 3544, 3866
MvnI CGCG 6 cut(s) 89, 335, 350, 395, 2063, 3166
NaeI GCCGGC 2 cut(s) 159, 639
NciI CCSGG 5 cut(s) 315, 316, 409, 1539, 1751
NdeI CATATG 1 cut(s) 1927
NdeII GATC 9 cut(s) 1406, 1541, 1730, 2905, 3125, 3136, 3259, 3535, 3868
NgoMIV GCCGGC 2 cut(s) 157, 637
NheI GCTAGC 1 cut(s) 3214
NmeAIII GCCGAG 3 cut(s) 434, 680, 1718
NmuCI GTSAC 1 cut(s) 2853
NsiI ATGCAT 2 cut(s) 613, 2591
NspI RCATGY 4 cut(s) 1893, 2451, 2611, 3817
NspV TTCGAA 1 cut(s) 260
PaeR7I CTCGAG 1 cut(s) 1376
PagI TCATGA 1 cut(s) 3369
PceI AGGCCT 1 cut(s) 1719
PciI ACATGT 1 cut(s) 3813
PciSI GCTCTTC 1 cut(s) 4331
PcsI WCGNNNNNNNCGW 1 cut(s) 3157
PctI GAATGC 5 cut(s) 35, 1573, 1939, 2476, 3427
PdiI GCCGGC 2 cut(s) 159, 639
PdmI GAANNNNTTC 2 cut(s) 1059, 3120
Ple19I CGATCG 1 cut(s) 3128
PleI GAGTC 7 cut(s) 502, 1152, 1256, 1387, 2821, 2860, 3381
PmaCI CACGTG 2 cut(s) 240, 3665
PmlI CACGTG 2 cut(s) 240, 3665
PpsI GAGTC 7 cut(s) 502, 1152, 1256, 1387, 2821, 2860, 3381
Ppu21I YACGTR 5 cut(s) 240, 2726, 3113, 3530, 3665
PpuMI RGGWCCY 3 cut(s) 1472, 2433, 2888
PscI ACATGT 1 cut(s) 3813
PshBI ATTAAT 1 cut(s) 2991
PsiI TTATAA 1 cut(s) 1896
Psp124BI GAGCTC 2 cut(s) 423, 4326
Psp5II RGGWCCY 3 cut(s) 1472, 2433, 2888
Psp6I CCWGG 8 cut(s) 340, 1152, 1474, 2143, 2370, 2755, 3542, 3864
PspCI CACGTG 2 cut(s) 240, 3665
PspFI CCCAGC 4 cut(s) 479, 986, 4058, 4254
PspGI CCWGG 8 cut(s) 340, 1152, 1474, 2143, 2370, 2755, 3542, 3864
PspPPI RGGWCCY 3 cut(s) 1472, 2433, 2888
PspXI VCTCGAGB 1 cut(s) 1376
PstI CTGCAG 1 cut(s) 633
PstNI CAGNNNCTG 2 cut(s) 1475, 2762
PsuI RGATCY 3 cut(s) 1541, 1730, 3868
PvuI CGATCG 1 cut(s) 3128
PvuII CAGCTG 4 cut(s) 1985, 2072, 2456, 3940
RsaI GTAC 8 cut(s) 281, 1810, 2461, 2712, 3032, 3401, 3528, 4307
RsaNI GTAC 8 cut(s) 280, 1809, 2460, 2711, 3031, 3400, 3527, 4306
RseI CAYNNNNRTG 5 cut(s) 616, 1425, 3070, 3111, 3890
SacI GAGCTC 2 cut(s) 423, 4326
SalI GTCGAC 2 cut(s) 622, 664
SapI GCTCTTC 1 cut(s) 4331
Sau3AI GATC 9 cut(s) 1406, 1541, 1730, 2905, 3125, 3136, 3259, 3535, 3868
SchI GAGTC 7 cut(s) 502, 1153, 1256, 1388, 2822, 2861, 3382
SduI GDGCHC 6 cut(s) 423, 2494, 2691, 2871, 3585, 4326
SfaNI GCATC 8 cut(s) 598, 991, 1320, 1583, 1802, 2349, 3766, 4177
SfcI CTRYAG 3 cut(s) 629, 910, 1519
Sfr274I CTCGAG 1 cut(s) 1376
SfuI TTCGAA 1 cut(s) 260
SinI GGWCC 9 cut(s) 1472, 1535, 1556, 1758, 2433, 2888, 3153, 4043, 4294
SlaI CTCGAG 1 cut(s) 1376
SmaI CCCGGG 1 cut(s) 316
SmiMI CAYNNNNRTG 5 cut(s) 616, 1425, 3070, 3111, 3890
SmlI CTYRAG 1 cut(s) 1376
SmoI CTYRAG 1 cut(s) 1376
SseBI AGGCCT 1 cut(s) 1719
SspMI CTAG 9 cut(s) 72, 642, 1383, 2189, 2810, 3215, 3488, 4047, 4259
SstI GAGCTC 2 cut(s) 423, 4326
StuI AGGCCT 1 cut(s) 1719
StyI CCWWGG 4 cut(s) 2660, 2909, 3235, 3991
TaqII GACCGA 2 cut(s) 181, 2660
TatI WGTACW 4 cut(s) 279, 1808, 3030, 4305
TauI GCSGC 5 cut(s) 157, 181, 490, 1574, 3087
TscAI CASTG 8 cut(s) 1831, 1861, 2305, 2722, 3017, 3086, 3352, 3616
TseFI GTSAC 1 cut(s) 2853
TseI GCWGC 7 cut(s) 330, 345, 387, 628, 3076, 3194, 4231
Tsp45I GTSAC 1 cut(s) 2853
TspGWI ACGGA 3 cut(s) 558, 2349, 3777
TspMI CCCGGG 1 cut(s) 314
TspRI CASTG 8 cut(s) 1831, 1861, 2305, 2722, 3017, 3086, 3352, 3616
VneI GTGCAC 1 cut(s) 2867
VpaK11BI GGWCC 9 cut(s) 1472, 1535, 1556, 1758, 2433, 2888, 3153, 4043, 4294
VspI ATTAAT 1 cut(s) 2991
XagI CCTNNNNNAGG 2 cut(s) 2550, 3625
XapI RAATTY 5 cut(s) 933, 1070, 1321, 3116, 3736
XceI RCATGY 4 cut(s) 1893, 2451, 2611, 3817
XcmI CCANNNNNNNNNTGG 3 cut(s) 2326, 2378, 3872
XhoI CTCGAG 1 cut(s) 1376
XmaI CCCGGG 1 cut(s) 314
XmiI GTMKAC 2 cut(s) 623, 665
XmnI GAANNNNTTC 2 cut(s) 1059, 3120
XspI CTAG 9 cut(s) 72, 642, 1383, 2189, 2810, 3215, 3488, 4047, 4259
ZraI GACGTC 2 cut(s) 595, 3964
Zsp2I ATGCAT 2 cut(s) 613, 2591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.