Rw2G006120

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002500
Physical Location & Seq
Forward (+)
5929629 .. 5939778
10150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006120.1

Sequence Viewer

Length: 4293 bp
ATGGTTCCTTTTGCCTCAGTTCTCCACCACCCTTTCATTCTCATCCTCTTCACCCTTTTCACCATAAACCCTACTCTCTCCACTGATTTGGGCAATGAATTCTCAATCCTTAGCTTCGATTCCGATGACTTTCGTGGAGACTACGCGCCTCCATCGCCACCGCCGAGTCCGCCGGAGCCTCAGCCGCCCTCACTCTCCTGCAGTGAGGGTCTCAATGCAACTGGGTCTTTCGACACCATTTGTGAGCTCAATTCAAGCTTGAGTCTTGAAAATGATGTGTATATACATGGAAATGGGAGCTTTTATATCCTCCCTGGTGTTAATGTGAGCTGCCCACGTTTGGGGTGCTCCATTGTGGTTAATATTAGTGGAGGTTTTAGTTTAGGTAGCAATTCAGTGATTATTGCCGGGTTAGTTTCGGTTAATGCTTCCAATGTTAGCTTGTTTACTGGTTCAGTGATTAATGTGACCGGGATGGCGGGGGCGCCGCCTCCAATGACTAGTGGCACACCAGGGGGTGAGCAGGGTGCTGGTGGAGGTCATGGTGGGAGAGGAGCTAGTTGTGTCACAGATAACAAGAAACTTCCGGATGATATTTGGGGTGGGGATCCATATGCTTGGTCATCGTTGGAGGAGCCGAAAAGTTATGGGAGTAAGGGTGGGAGTACCAGTAAGGAAGTGAAGTATGGTGGGGAAGGAGGTGGAAGGATTTGGTTGGAGACGAGAAACTCGATTGTTGTTGGGGGGAGTGTTTTGGCTGATGGTGGTGATGGTGGTATCAAGGGTGGAGGAGGGTCAGGAGGCAGCATATACATCAAAGGTCTTAGAATGACTGGAAGTGGCATGATAAGTGCAATTGGGGGTAATGGATTTGCTGGAGGTGGAGGTGGAAGAATTTCTATCAATGTTTTCAGCAGGCGTGATGAACCAGAAATCTTTAGTCATGGGGGAAGGAGTTTAGGCTGTCCTGAAAATGCAGGTGCTGCAGGGACATATTATGATGCTGTCCCTCGAAGGCTTATAGTCAGTAATCATAACTTGTCCACACGGACTGACACGCTTCTGTTGGAGTTTCCTAAACTGCCCCTCTGGACAAATGTTGATATACAAGATCATGCCAAGGCTCTGGTTCCTCTGTTTTGGAGCCGTGTTCAGGTTAGAGGTCAAATTCGTTTATCATGTGGTGCAGTTTTGAGTTTTGGTCTTCAACATTTTGCTTCATCGGAGTTTGAGTTAATGGCAGAAGAGCTTGTAATGAGTGATTCTATCGTCAAGATATTTGGGGCTCTTCGAATGTCTGTGAAAATGCACTTGATGTGGAATTCCAAAATGCTTATAGAGGGTGGTGTTGATGCACTTGTGGCAACATCCTTGCTTGAAGCAACCAATTTAATGGTTCTCAGGGAGTCCTCTGTGATACATTCTACTGCGAATTTGGGAGTTCATGGACAGGGTTTCATGAATTTGACTGGGCCAGGAGATGTTATTGAAGCAAAACACCTGGTTCTGTCTCTATTTTTTAGTATCAATGTTGGACCTGGATCTTTTCTGCGAGGGCCTTTGGATCGCAGCAGTAGTAACTTTACGACACCCCAACTCAACTGTGAGCTCCCAAATTGCCCTGTGGAATTACTTCATCCACCTGAAGATTGTAACATGAACTCTACATTGACCTTCACTCTTCAGATTTGTCGAGTTGAATATGTTATTGTTGAAGGCATTATAACTGGATCTGTTATACATTTTCACTGGGTGAGAGATGTAGTTGTACACTCTTCTGGGGTAATTACTGCATCTGGTCTGGGTTGCACTGGTGGGCTGGGTAGAGGAAGATTTTTTGTAAATGGTCTTGGCAGCGGTGGCGGGCATGGAGGCAAAGGTGGAGATGGGTTTTACGATGGAAATTTTATTGAAGGTGGTGTTTCCTATGGAAATGCTGATTTACCTTGTGAGCTTGGTAGTGGTAGTGGAAATGATAGTCTAGCTGGTGCAACAGCAGGTGGTGGTATTATTGTTTCACTCTTGCGGGCTCTCTTAGAGCTGATGGAGAAACCTTTGGAGAAAATTTTGGGGAGAAAAGTTGGTCAGACCATTTCAAATATAGGTCCTGGCGGTGGATCCGGTGGAACCGTTCTTTTGTTTGTTCAGACATTAGCACTCGGTGATTCTTCTACAATCTCAACTGTTGGTGGGCATGGTAGTCCTAGTGGTGGCGGTGGAGGAGGTGGTGGGAGGATTCACTTCCATTGGTCGGACATACCAGTTGGGGATGCATATCTACCCATAGCAAGCGTGCGAGGAAACATTAATACTGGGGGAGGATTCGGTAGAGGAAATGGGCGTGCTGGACAAAATGGGACTATCACTGGAAGGGCCTGTCCCAGAGGGTTATATGGTATCTTTTGTGAGGAATGCCCTCTTGGCACCTTTAAGAATGTCAGTGGCTCTGCTAGAGCCCTTTGTCATGCTTGTCCTTCTTTTCAGCTTCCGCATCGTGCCATATATGTTACCGTTCGAGGTGGTGTTACTGAGACTCCATGTCCATACAAGTGCGTTTCTGACAGATATCACATGCCAAACTGTTATACAGCAGTTGAAGAATTGGTATACACTTTCGGTGGACCATGGTTGTTCTGTCTTATTCTGTTAGGTCTCCTCGTCCTCTTAGCTCTTGTGCTCAGTGTTGCACGAATGAAATATGTTACTGCAGACGAATTACCGGCTGCTGTACCTGCTCGACGGGGCTCCCGTATAGATCATTCTTTCCCTTTCCTTGAATCGTTAAATGAGGTTTTGGAAACAAATAGAAATGAGGAATCTCAGAGTCATGTGCATAGGATGTATTTCATGGGGCCAAATACATTCAGTGAACCTTGGCATCTACCTCATTCCCCTCCAGAACAAATAACAGAGATAGTATATGAGGATGCATTCAATCGGTTTGTGGATGAGATTAATGGTTTAGCTGCTTATCAGTGGTGGGAAGGATCAGTCTACAGCATTCTTTCTATTCTTGCCTATCCACTTGCTTGGTGTTGGCTACAGAGTCGACGGAAACAGAAATTACAACAACTTCGTGAATTTGTTCGATCAGAATATGATCATTCTTGCTTGCGTTCTTGTCGTTCCCGTGCACTATATGAAGGGATTAAGGTGGCTGCAACTACGGATTTGATGCTTGCATATGTGGACTTTTTCCTAGGTGGAGATGAGAAAAGAGCTGGCCTTCCTCCCCGTCTTCATCAGAGATTTCCAATGTCTATAATTTTTGGAGGAGATGGAAGTTACATGGCTCCTTTCTCTCTTCAGAGTGATAACATTCTTACTAGCCTCATGAGTCAGTCTGTTCCACCTACCATCTGGTATCGACTTGTAGCCGGTCTAAATGCTCAGTTGCGCTTAGTCCGGCATGGACACTTAAAAATGACTTTTAGTCATGTTACTAACTGGCTTGAAACACATGCAAAACCTACTCTTGGTGCATTTGGCATACATGTTGATCTTGCCTGGTTTCAGCCTACAGCGTCTGGATATTGCCACTTCGGAATCGTGGTTTATGCTATGGAAGTTGAAAGTCTGCCATCTGAATTGGACGGTCAAGGTGCATTCTTTCTACCAGAGCATCAATCACGCATGCCAAGAAATCATTGGGAAAACCAAATTGAACATTTGAGACTCATTGACCTTTGGATATCTCAAAAGAAGTTTTCTGGAGGGATTTTACACACCAAAGGCTTGAGGACGTTAAAAGAGAAGAAGGCCATATGTTATCCCTTCTCTTTTATACTTTATAATGCTAAACCCATTGGCCATCAGGATCTTGTTGGTTTGGTCATTTCTATTTTACTCTTGGGAGACTTTAGCTTAGTTTTGCTCACTTTGCTCCAGCTGTATTCTATTTCACTCTTAAGTTTATTCTTAGTCTTGTTCATTCTTCCTCTCGGTCTACTATTCCCCTTCCCGGCTGGAATCAGCGCTTTGTTTAGTCACGGGGCTAGACGTTCAGCAGGTCTTGCCCGTTTATATGCTTTGTGGAATCTCACATCCTTGATCAATGTTGTAGTTGCTTTTATATGTGGATTGATTCATTATACGACTCACTCAAATAAAAAGCATTCCAATTTTCAGTCCTGGACTTTTAGCATGGATGAAAGTGAGTGGTGGGTGCTTCCTTGTGGTCTAGTACTGTGCAAAGTTGTCCAATCACGACTTATTGATTGCCTCGTGGCAAACCAGGAGATTCAGGATCACTCACTGTACAGCAATGACCCTGAACTGTTCTGGCAGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1430

Amino Acids

153.94

Weight (kDa)

6.29

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8003 PF26010 788 - 862 1.5e-34 Domain of unknown function (DUF8003)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1724, 3792
AarI CACCTGC 2 cut(s) 968, 1988
Acc36I ACCTGC 4 cut(s) 968, 1988, 2740, 3998
AccB1I GGYRCC 2 cut(s) 484, 2423
AccI GTMKAC 4 cut(s) 2607, 2994, 3049, 3946
AccII CGCG 1 cut(s) 146
AccIII TCCGGA 1 cut(s) 586
AcoI YGGCCR 1 cut(s) 3808
AcsI RAATTY 9 cut(s) 98, 894, 1167, 1321, 1432, 1462, 1903, 2064, 3080
AcuI CTGAAG 3 cut(s) 1665, 1667, 3290
AcyI GRCGYC 1 cut(s) 485
AdeI CACNNNGTG 2 cut(s) 1753, 2162
AfaI GTAC 5 cut(s) 667, 1770, 2730, 4185, 4259
AfeI AGCGCT 1 cut(s) 3976
AfiI CCNNNNNNNGG 8 cut(s) 340, 341, 1153, 2114, 2210, 2251, 3476, 3961
AflII CTTAAG 1 cut(s) 3907
AflIII ACRYGT 1 cut(s) 3493
AhdI GACNNNNNGTC 2 cut(s) 2538, 3432
AhlI ACTAGT 1 cut(s) 500
AjnI CCWGG 9 cut(s) 313, 511, 1474, 1500, 1537, 2107, 3506, 4130, 4233
AjuI GAANNNNNNNTTGG 2 cut(s) 2403, 2435
Alw21I GWGCWC 5 cut(s) 249, 350, 1611, 2679, 3136
Alw26I GTCTC 8 cut(s) 132, 215, 713, 1515, 2525, 2657, 3667, 3849
Alw44I GTGCAC 1 cut(s) 3132
AlwNI CAGNNNCTG 2 cut(s) 983, 3527
Aor13HI TCCGGA 1 cut(s) 586
Aor51HI AGCGCT 1 cut(s) 3976
AoxI GGCC 7 cut(s) 1472, 1556, 2373, 2852, 3223, 3759, 3808
ApaLI GTGCAC 1 cut(s) 3132
ApeKI GCWGC 8 cut(s) 330, 804, 983, 1569, 1854, 2723, 2966, 3158
ApoI RAATTY 9 cut(s) 98, 894, 1167, 1321, 1432, 1462, 1903, 2064, 3080
AseI ATTAAT 3 cut(s) 462, 2307, 2955
Asp700I GAANNNNTTC 3 cut(s) 895, 1632, 3084
AspA2I CCTAGG 1 cut(s) 3199
AspLEI GCGC 4 cut(s) 148, 487, 3399, 3977
AspS9I GGNCC 7 cut(s) 1472, 1535, 1556, 2105, 2373, 2621, 2852
AsuC2I CCSGG 3 cut(s) 409, 472, 3962
AsuHPI GGTGA 6 cut(s) 43, 52, 530, 779, 1765, 2174
AsuII TTCGAA 1 cut(s) 1291
AvaII GGWCC 3 cut(s) 1535, 2105, 2621
AvrII CCTAGG 1 cut(s) 3199
BaeGI GKGCMC 1 cut(s) 3136
BalI TGGCCA 1 cut(s) 3810
BamHI GGATCC 2 cut(s) 607, 2117
BanI GGYRCC 2 cut(s) 484, 2423
BanII GRGCYC 6 cut(s) 249, 1288, 1611, 2032, 2458, 2747
BauI CACGAG 1 cut(s) 4223
BbsI GAAGAC 2 cut(s) 1196, 3230
Bbv12I GWGCWC 5 cut(s) 249, 350, 1611, 2679, 3136
BbvCI CCTCAGC 1 cut(s) 180
BbvI GCAGC 8 cut(s) 317, 816, 970, 1581, 1866, 2710, 2953, 3145
BceAI ACGGC 1 cut(s) 1131
BciT130I CCWGG 9 cut(s) 315, 513, 1476, 1502, 1539, 2109, 3508, 4132, 4235
BclI TGATCA 2 cut(s) 3100, 4050
BcnI CCSGG 3 cut(s) 409, 472, 3962
BcoDI GTCTC 8 cut(s) 132, 215, 713, 1515, 2525, 2657, 3667, 3849
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 9 cut(s) 501, 558, 1982, 2205, 2451, 3200, 3327, 3996, 4181
BfmI CTRYAG 6 cut(s) 199, 984, 2706, 2995, 3041, 3519
BfoI RGCGCY 2 cut(s) 488, 3978
BfrI CTTAAG 1 cut(s) 3907
BfuAI ACCTGC 4 cut(s) 968, 1988, 2740, 3998
BglI GCCNNNNNGGC 1 cut(s) 2421
BlnI CCTAGG 1 cut(s) 3199
BmcAI AGTACT 1 cut(s) 4185
Bme18I GGWCC 3 cut(s) 1535, 2105, 2621
BmeRI GACNNNNNGTC 2 cut(s) 2538, 3432
BmgT120I GGNCC 7 cut(s) 1472, 1535, 1556, 2105, 2373, 2621, 2852
BmrI ACTGGG 4 cut(s) 231, 1479, 1759, 2322
BmsI GCATC 9 cut(s) 991, 1342, 1802, 2260, 2500, 2887, 2917, 3165, 3631
BmuI ACTGGG 4 cut(s) 231, 1479, 1759, 2322
BpiI GAAGAC 2 cut(s) 1196, 3230
BpmI CTGGAG 4 cut(s) 897, 2880, 3732, 3869
Bpu10I CCTNAGC 2 cut(s) 110, 180
Bpu14I TTCGAA 1 cut(s) 1291
BpuEI CTTGAG 2 cut(s) 280, 3757
BpuMI CCSGG 3 cut(s) 409, 472, 3962
BsaBI GATNNNNATC 1 cut(s) 2274
BsaHI GRCGYC 1 cut(s) 485
BsaI GGTCTC 2 cut(s) 215, 2657
BsaJI CCNNGG 6 cut(s) 313, 512, 1119, 2624, 2873, 3199
BsaWI WCCGGW 2 cut(s) 586, 2120
BsaXI ACNNNNNCTCC 8 cut(s) 643, 655, 673, 685, 1217, 1247, 2518, 2548
Bsc4I CCNNNNNNNGG 8 cut(s) 340, 341, 1153, 2114, 2210, 2251, 3476, 3961
Bse118I RCCGGY 2 cut(s) 2719, 3377
Bse3DI GCAATG 2 cut(s) 100, 4270
Bse8I GATNNNNATC 1 cut(s) 2274
BseAI TCCGGA 1 cut(s) 586
BseBI CCWGG 9 cut(s) 315, 513, 1476, 1502, 1539, 2109, 3508, 4132, 4235
BseDI CCNNGG 6 cut(s) 313, 512, 1119, 2624, 2873, 3199
BseJI GATNNNNATC 1 cut(s) 2274
BseLI CCNNNNNNNGG 8 cut(s) 340, 341, 1153, 2114, 2210, 2251, 3476, 3961
BseMI GCAATG 2 cut(s) 100, 4270
BseMII CTCAG 7 cut(s) 30, 194, 1414, 2520, 2692, 2834, 3404
BseRI GAGGAG 6 cut(s) 567, 647, 804, 2235, 2645, 3288
BseSI GKGCMC 1 cut(s) 3136
BseXI GCAGC 8 cut(s) 317, 816, 970, 1581, 1866, 2710, 2953, 3145
BseYI CCCAGC 1 cut(s) 1819
BsgI GTGCAG 1 cut(s) 1206
Bsh1236I CGCG 1 cut(s) 146
BshFI GGCC 7 cut(s) 1474, 1558, 2375, 2854, 3225, 3761, 3810
BshNI GGYRCC 2 cut(s) 484, 2423
BsiHKAI GWGCWC 5 cut(s) 249, 350, 1611, 2679, 3136
BsiSI CCGG 9 cut(s) 173, 408, 471, 587, 2121, 2720, 3378, 3406, 3962
BslFI GGGAC 4 cut(s) 992, 1003, 2364, 2371
BslI CCNNNNNNNGG 8 cut(s) 340, 341, 1153, 2114, 2210, 2251, 3476, 3961
BsmAI GTCTC 8 cut(s) 132, 215, 713, 1515, 2525, 2657, 3667, 3849
BsmBI CGTCTC 1 cut(s) 713
BsmFI GGGAC 4 cut(s) 992, 1003, 2364, 2371
BsmI GAATGC 5 cut(s) 2417, 2930, 3000, 3605, 4114
BsnI GGCC 7 cut(s) 1474, 1558, 2375, 2854, 3225, 3761, 3810
Bso31I GGTCTC 2 cut(s) 215, 2657
Bsp119I TTCGAA 1 cut(s) 1291
Bsp1286I GDGCHC 9 cut(s) 249, 350, 1288, 1611, 2032, 2458, 2679, 2747, 3136
Bsp13I TCCGGA 1 cut(s) 586
Bsp1407I TGTACA 2 cut(s) 1768, 4257
Bsp19I CCATGG 1 cut(s) 2624
BspANI GGCC 7 cut(s) 1474, 1558, 2375, 2854, 3225, 3761, 3810
BspCNI CTCAG 7 cut(s) 29, 193, 1413, 2521, 2691, 2833, 3403
BspEI TCCGGA 1 cut(s) 586
BspFNI CGCG 1 cut(s) 146
BspHI TCATGA 2 cut(s) 1458, 3333
BspMAI CTGCAG 3 cut(s) 203, 988, 2710
BspMI ACCTGC 4 cut(s) 968, 1988, 2740, 3998
BspQI GCTCTTC 2 cut(s) 1239, 1293
BspT104I TTCGAA 1 cut(s) 1291
BspT107I GGYRCC 2 cut(s) 484, 2423
BspTI CTTAAG 1 cut(s) 3907
BspTNI GGTCTC 2 cut(s) 215, 2657
BsrDI GCAATG 2 cut(s) 100, 4270
BsrFI RCCGGY 2 cut(s) 2719, 3377
BsrGI TGTACA 2 cut(s) 1768, 4257
BssAI RCCGGY 2 cut(s) 2719, 3377
BssECI CCNNGG 6 cut(s) 313, 512, 1119, 2624, 2873, 3199
BssNAI GTATAC 1 cut(s) 2608
BssNI GRCGYC 1 cut(s) 485
BssSI CACGAG 1 cut(s) 4223
BssT1I CCWWGG 4 cut(s) 1119, 2624, 2873, 3199
Bst1107I GTATAC 1 cut(s) 2608
Bst2BI CACGAG 1 cut(s) 4223
Bst2UI CCWGG 9 cut(s) 315, 513, 1476, 1502, 1539, 2109, 3508, 4132, 4235
Bst4CI ACNGT 9 cut(s) 1604, 2131, 2185, 2512, 2582, 3596, 4188, 4257, 4278
Bst6I CTCTTC 6 cut(s) 53, 1239, 1293, 1686, 1780, 3309
BstACI GRCGYC 1 cut(s) 485
BstAFI CTTAAG 1 cut(s) 3907
BstAPI GCANNNNNTGC 2 cut(s) 983, 4013
BstAUI TGTACA 2 cut(s) 1768, 4257
BstBI TTCGAA 1 cut(s) 1291
BstDSI CCRYGG 1 cut(s) 2624
BstFNI CGCG 1 cut(s) 146
BstH2I RGCGCY 2 cut(s) 488, 3978
BstHHI GCGC 4 cut(s) 148, 487, 3399, 3977
BstMAI GTCTC 8 cut(s) 132, 215, 713, 1515, 2525, 2657, 3667, 3849
BstNI CCWGG 9 cut(s) 315, 513, 1476, 1502, 1539, 2109, 3508, 4132, 4235
BstNSI RCATGY 4 cut(s) 2575, 3464, 3497, 3637
BstSFI CTRYAG 6 cut(s) 199, 984, 2706, 2995, 3041, 3519
BstSLI GKGCMC 1 cut(s) 3136
BstUI CGCG 1 cut(s) 146
BstV1I GCAGC 8 cut(s) 317, 816, 970, 1581, 1866, 2710, 2953, 3145
BstV2I GAAGAC 2 cut(s) 1196, 3230
BstX2I RGATCY 5 cut(s) 607, 1541, 1730, 2117, 3817
BstXI CCANNNNNNTGG 5 cut(s) 88, 618, 1126, 1393, 3030
BstYI RGATCY 5 cut(s) 607, 1541, 1730, 2117, 3817
BstZ17I GTATAC 1 cut(s) 2608
BsuRI GGCC 7 cut(s) 1474, 1558, 2375, 2854, 3225, 3761, 3810
BtgI CCRYGG 1 cut(s) 2624
BtgZI GCGATG 1 cut(s) 138
BtsI GCAGTG 1 cut(s) 208
BveI ACCTGC 4 cut(s) 968, 1988, 2740, 3998
CaiI CAGNNNCTG 2 cut(s) 983, 3527
CciI TCATGA 2 cut(s) 1458, 3333
CfoI GCGC 4 cut(s) 148, 487, 3399, 3977
Cfr10I RCCGGY 2 cut(s) 2719, 3377
Cfr13I GGNCC 7 cut(s) 1472, 1535, 1556, 2105, 2373, 2621, 2852
CseI GACGC 1 cut(s) 3513
CsiI ACCWGGT 1 cut(s) 1500
Csp6I GTAC 5 cut(s) 666, 1769, 2729, 4184, 4258
CviQI GTAC 5 cut(s) 666, 1769, 2729, 4184, 4258
DinI GGCGCC 1 cut(s) 486
DraIII CACNNNGTG 2 cut(s) 1753, 2162
DriI GACNNNNNGTC 2 cut(s) 2538, 3432
EaeI YGGCCR 1 cut(s) 3808
Eam1104I CTCTTC 6 cut(s) 53, 1239, 1293, 1686, 1780, 3309
Eam1105I GACNNNNNGTC 2 cut(s) 2538, 3432
EarI CTCTTC 6 cut(s) 53, 1239, 1293, 1686, 1780, 3309
EciI GGCGGA 1 cut(s) 159
Ecl136II GAGCTC 2 cut(s) 247, 1609
Eco130I CCWWGG 4 cut(s) 1119, 2624, 2873, 3199
Eco24I GRGCYC 6 cut(s) 249, 1288, 1611, 2032, 2458, 2747
Eco31I GGTCTC 2 cut(s) 215, 2657
Eco32I GATATC 2 cut(s) 2567, 3693
Eco47I GGWCC 3 cut(s) 1535, 2105, 2621
Eco47III AGCGCT 1 cut(s) 3976
Eco53kI GAGCTC 2 cut(s) 247, 1609
Eco57I CTGAAG 3 cut(s) 1665, 1667, 3290
EcoICRI GAGCTC 2 cut(s) 247, 1609
EcoO109I RGGNCCY 3 cut(s) 1556, 2105, 2373
EcoRI GAATTC 2 cut(s) 98, 1321
EcoRII CCWGG 9 cut(s) 313, 511, 1474, 1500, 1537, 2107, 3506, 4130, 4233
EcoRV GATATC 2 cut(s) 2567, 3693
EcoT14I CCWWGG 4 cut(s) 1119, 2624, 2873, 3199
EcoT22I ATGCAT 2 cut(s) 2275, 2932
EcoT38I GRGCYC 6 cut(s) 249, 1288, 1611, 2032, 2458, 2747
EgeI GGCGCC 1 cut(s) 486
EheI GGCGCC 1 cut(s) 486
ErhI CCWWGG 4 cut(s) 1119, 2624, 2873, 3199
Esp3I CGTCTC 1 cut(s) 713
FaqI GGGAC 4 cut(s) 992, 1003, 2364, 2371
FauI CCCGC 3 cut(s) 472, 1856, 2019
FauNDI CATATG 3 cut(s) 613, 3184, 3764
FbaI TGATCA 2 cut(s) 3100, 4050
FblI GTMKAC 4 cut(s) 2607, 2994, 3049, 3946
FriOI GRGCYC 6 cut(s) 249, 1288, 1611, 2032, 2458, 2747
FspBI CTAG 9 cut(s) 501, 558, 1982, 2205, 2451, 3200, 3327, 3996, 4181
GlaI GCGC 4 cut(s) 147, 486, 3398, 3976
GsaI CCCAGC 1 cut(s) 1823
GsuI CTGGAG 4 cut(s) 897, 2880, 3732, 3869
HaeII RGCGCY 2 cut(s) 488, 3978
HaeIII GGCC 7 cut(s) 1474, 1558, 2375, 2854, 3225, 3761, 3810
HapII CCGG 9 cut(s) 173, 408, 471, 587, 2121, 2720, 3378, 3406, 3962
HgaI GACGC 1 cut(s) 3513
HhaI GCGC 4 cut(s) 148, 487, 3399, 3977
Hin1I GRCGYC 1 cut(s) 485
Hin6I GCGC 4 cut(s) 146, 485, 3397, 3975
HinP1I GCGC 4 cut(s) 146, 485, 3397, 3975
HincII GTYRAC 1 cut(s) 3050
HindII GTYRAC 1 cut(s) 3050
HindIII AAGCTT 1 cut(s) 256
HpaII CCGG 9 cut(s) 173, 408, 471, 587, 2121, 2720, 3378, 3406, 3962
HphI GGTGA 6 cut(s) 43, 52, 530, 779, 1765, 2174
Hpy99I CGWCG 2 cut(s) 2742, 3054
HpyCH4III ACNGT 9 cut(s) 1604, 2131, 2185, 2512, 2582, 3596, 4188, 4257, 4278
HpyCH4IV ACGT 3 cut(s) 337, 3743, 4000
HpySE526I ACGT 3 cut(s) 337, 3743, 4000
Hsp92I GRCGYC 1 cut(s) 485
HspAI GCGC 4 cut(s) 146, 485, 3397, 3975
KasI GGCGCC 1 cut(s) 484
Kpn2I TCCGGA 1 cut(s) 586
Ksp22I TGATCA 2 cut(s) 3100, 4050
LguI GCTCTTC 2 cut(s) 1239, 1293
Lsp1109I GCAGC 8 cut(s) 317, 816, 970, 1581, 1866, 2710, 2953, 3145
LweI GCATC 9 cut(s) 991, 1342, 1802, 2260, 2500, 2887, 2917, 3165, 3631
MabI ACCWGGT 1 cut(s) 1500
MaeI CTAG 9 cut(s) 501, 558, 1982, 2205, 2451, 3200, 3327, 3996, 4181
MaeII ACGT 3 cut(s) 337, 3743, 4000
MfeI CAATTG 1 cut(s) 855
MflI RGATCY 5 cut(s) 607, 1541, 1730, 2117, 3817
MhlI GDGCHC 9 cut(s) 249, 350, 1288, 1611, 2032, 2458, 2679, 2747, 3136
MlsI TGGCCA 1 cut(s) 3810
MluNI TGGCCA 1 cut(s) 3810
Mly113I GGCGCC 1 cut(s) 485
MlyI GAGTC 9 cut(s) 175, 271, 1415, 2527, 2833, 3055, 3346, 3669, 4090
MmeI TCCRAC 5 cut(s) 609, 696, 1047, 1513, 2232
Mox20I TGGCCA 1 cut(s) 3810
Mph1103I ATGCAT 2 cut(s) 2275, 2932
MroI TCCGGA 1 cut(s) 586
MroXI GAANNNNTTC 3 cut(s) 895, 1632, 3084
MscI TGGCCA 1 cut(s) 3810
MslI CAYNNNNRTG 2 cut(s) 291, 2548
Msp20I TGGCCA 1 cut(s) 3810
MspA1I CMGCKG 2 cut(s) 1857, 3889
MspCI CTTAAG 1 cut(s) 3907
MspI CCGG 9 cut(s) 173, 408, 471, 587, 2121, 2720, 3378, 3406, 3962
MunI CAATTG 1 cut(s) 855
Mva1269I GAATGC 5 cut(s) 2417, 2930, 3000, 3605, 4114
MvaI CCWGG 9 cut(s) 315, 513, 1476, 1502, 1539, 2109, 3508, 4132, 4235
MvnI CGCG 1 cut(s) 146
NarI GGCGCC 1 cut(s) 485
NciI CCSGG 3 cut(s) 409, 472, 3962
NcoI CCATGG 1 cut(s) 2624
NdeI CATATG 3 cut(s) 613, 3184, 3764
NmeAIII GCCGAG 1 cut(s) 189
NmuCI GTSAC 3 cut(s) 466, 565, 3986
NsiI ATGCAT 2 cut(s) 2275, 2932
NspI RCATGY 4 cut(s) 2575, 3464, 3497, 3637
NspV TTCGAA 1 cut(s) 1291
PaeI GCATGC 1 cut(s) 3637
PagI TCATGA 2 cut(s) 1458, 3333
PaqCI CACCTGC 2 cut(s) 968, 1988
PciI ACATGT 1 cut(s) 3493
PciSI GCTCTTC 2 cut(s) 1239, 1293
PcsI WCGNNNNNNNCGW 3 cut(s) 161, 728, 2746
PctI GAATGC 5 cut(s) 2417, 2930, 3000, 3605, 4114
PdmI GAANNNNTTC 3 cut(s) 895, 1632, 3084
PfoI TCCNGGA 1 cut(s) 4130
PleI GAGTC 9 cut(s) 174, 270, 1414, 2527, 2832, 3054, 3345, 3669, 4090
PluTI GGCGCC 1 cut(s) 488
PpsI GAGTC 9 cut(s) 174, 270, 1414, 2527, 2832, 3054, 3345, 3669, 4090
PpuMI RGGWCCY 1 cut(s) 2105
PscI ACATGT 1 cut(s) 3493
PshBI ATTAAT 3 cut(s) 462, 2307, 2955
PsiI TTATAA 2 cut(s) 1724, 3792
Psp124BI GAGCTC 2 cut(s) 249, 1611
Psp5II RGGWCCY 1 cut(s) 2105
Psp6I CCWGG 9 cut(s) 313, 511, 1474, 1500, 1537, 2107, 3506, 4130, 4233
PspFI CCCAGC 1 cut(s) 1819
PspGI CCWGG 9 cut(s) 313, 511, 1474, 1500, 1537, 2107, 3506, 4130, 4233
PspPI GGNCC 7 cut(s) 1472, 1535, 1556, 2105, 2373, 2621, 2852
PspPPI RGGWCCY 1 cut(s) 2105
PstI CTGCAG 3 cut(s) 203, 988, 2710
PstNI CAGNNNCTG 2 cut(s) 983, 3527
PsuI RGATCY 5 cut(s) 607, 1541, 1730, 2117, 3817
PvuII CAGCTG 1 cut(s) 3889
RsaI GTAC 5 cut(s) 667, 1770, 2730, 4185, 4259
RsaNI GTAC 5 cut(s) 666, 1769, 2729, 4184, 4258
RseI CAYNNNNRTG 2 cut(s) 291, 2548
SacI GAGCTC 2 cut(s) 249, 1611
SalI GTCGAC 1 cut(s) 3048
SapI GCTCTTC 2 cut(s) 1239, 1293
Sau96I GGNCC 7 cut(s) 1472, 1535, 1556, 2105, 2373, 2621, 2852
ScaI AGTACT 1 cut(s) 4185
SchI GAGTC 9 cut(s) 175, 271, 1415, 2527, 2833, 3055, 3346, 3669, 4090
SduI GDGCHC 9 cut(s) 249, 350, 1288, 1611, 2032, 2458, 2679, 2747, 3136
SexAI ACCWGGT 1 cut(s) 1500
SfaNI GCATC 9 cut(s) 991, 1342, 1802, 2260, 2500, 2887, 2917, 3165, 3631
SfcI CTRYAG 6 cut(s) 199, 984, 2706, 2995, 3041, 3519
SfoI GGCGCC 1 cut(s) 486
SfuI TTCGAA 1 cut(s) 1291
SinI GGWCC 3 cut(s) 1535, 2105, 2621
SmiMI CAYNNNNRTG 2 cut(s) 291, 2548
SmlI CTYRAG 3 cut(s) 259, 3736, 3907
SmoI CTYRAG 3 cut(s) 259, 3736, 3907
SpeI ACTAGT 1 cut(s) 500
SphI GCATGC 1 cut(s) 3637
SspDI GGCGCC 1 cut(s) 484
SspI AATATT 1 cut(s) 364
SspMI CTAG 9 cut(s) 501, 558, 1982, 2205, 2451, 3200, 3327, 3996, 4181
SstI GAGCTC 2 cut(s) 249, 1611
StyI CCWWGG 4 cut(s) 1119, 2624, 2873, 3199
TaaI ACNGT 9 cut(s) 1604, 2131, 2185, 2512, 2582, 3596, 4188, 4257, 4278
TaiI ACGT 3 cut(s) 340, 3746, 4003
TaqII GACCGA 1 cut(s) 3932
TatI WGTACW 3 cut(s) 1768, 4183, 4257
TauI GCSGC 2 cut(s) 187, 490
TseFI GTSAC 3 cut(s) 466, 565, 3986
TseI GCWGC 8 cut(s) 330, 804, 983, 1569, 1854, 2723, 2966, 3158
Tsp45I GTSAC 3 cut(s) 466, 565, 3986
TspGWI ACGGA 3 cut(s) 1063, 3067, 3182
Vha464I CTTAAG 1 cut(s) 3907
VneI GTGCAC 1 cut(s) 3132
VpaK11BI GGWCC 3 cut(s) 1535, 2105, 2621
VspI ATTAAT 3 cut(s) 462, 2307, 2955
XapI RAATTY 9 cut(s) 98, 894, 1167, 1321, 1432, 1462, 1903, 2064, 3080
XceI RCATGY 4 cut(s) 2575, 3464, 3497, 3637
XcmI CCANNNNNNNNNTGG 2 cut(s) 3357, 3645
XmaJI CCTAGG 1 cut(s) 3199
XmiI GTMKAC 4 cut(s) 2607, 2994, 3049, 3946
XmnI GAANNNNTTC 3 cut(s) 895, 1632, 3084
XspI CTAG 9 cut(s) 501, 558, 1982, 2205, 2451, 3200, 3327, 3996, 4181
ZrmI AGTACT 1 cut(s) 4185
Zsp2I ATGCAT 2 cut(s) 2275, 2932
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.