MD15G1200900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002500
Physical Location & Seq
Reverse (-)
15974424 .. 15982519
8096 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1200900.v1.1.491

Sequence Viewer

Length: 4314 bp
ATGGCTCAAAACCCCCCGTTTCTTCTCCACCTTTTCATCCTCAACCTCTTCATCAGTTTCGCCATAAACCCTAGTCTCTGCGCTGATTCGGACGATGATTTTTCGATTCTTGGGTTCGAAATTCATGGGGACTACTCGCCACCTTCGCCGCCTCCGAGTCCGCCGCCGCCACACCCTCCCCCGCTCTCGTGCCAGGAGGGTCTCAACGGAATTGGGTCTCTGGACACCACTTGCGAGCTGAATTCAAGCTTGATTCTTGAAGATAATGTGTATATAGAAGGAAATGGGAGCTTATATATACTTCCCGGTGTTAATTTGAGCTGCCCATTATTGGGTTGTGAAATTGTGGTTAACATTAGTGGGGAATTTAGTTTAGGTAGAAATTCAATGATTATAGCCGGGTTAGTTTCGGTTAATGCTTCGAATGCGAGCTTTTTAACTGGTTCAGTAATAAATGTTACGGCATTGGCTGGTGCACCCCCGGCGCAGACTAGTGGCACCCCGGATGGTGTTCAGGGTTCTGGTGGAGGGCATGGTGGGAGAGGTGCTAGTTGTGTGATTGATAATACAAAGCTCCCAGATGATGTTTGGGGTGGGGATCCGTATGCGTGGTCATCGTTGGATGAGCCAGTGAGTTATGGCAGTAAGGGTGGAACAACTAGTAAGGATGAGAAGTATGGTGGGGAAGGAGGTGGGAGGATTTGGTTGGAGGCAAGAAGTTCAATTGATCTTAGTGGGAGTGTTTTGGCTGATGGTGGTGATGGTGGGATTAAGGGTGGTGGAGGTTCAGGGGGCAGCATATTTGTTAAAGCTCGTAGAATGACTGGAAGTGGCAGGATAAGTGCAGTGGGAGGTAATGGATTTGCTGGAGGAGGAGGTGGAAGAGTTTCCATCAAAGTTTTCAGCAGGCGTGATGATACAGATATCTTCGCTCATGGGGGAAGGAGTTTAGGCTGTCTTGAAAATGCAGGTGCTGCGGGAACATATTATGATGCTGTACCTCGGAGACTTATAGTCAGTAATCATAACTTATCCACACAGACTGACACGCTTCTGCTAGAGTTCCCTAAACAGCCACTTTGGACAAATGTTGACATCCAAAATCATGCCAAGGCTTTGGTTCCTTTGTATTGGAGCCGTGTTCAGGTTCGAGGTCAAATTCGTTTATCATGTGGTGCAGTTTTGAGTTTTGGTCTTGCACATTTTGCTTCATCAGAATTTGAGTTGATGGCTGAAGAGCTTCTGATGAGTGATTCTATTGTCAAGATATTTGGGGCTCTTCGAATGTCTGTCAAAATGCACTTGATGTTGAATTCTAAAATGCTAATAGATGGTGGTGCTGATTCAATTGTGGCAACATCCTTACTTGAGGCAAGCAATTTAGTGGTTCTCAGGGGGGCGTCTGTGATACATTCTAATGCTAATTTGGGAGTTCACGGACAAGGTTTCTTGAATTTGTCTGGGCCAGGAGACCTGATTGAAGCACAACACCTGATTCTGTCTCTATTTTTTAGTATCAAGATTGGACCTGGATCTTTACTGCGAGGGCCCCTGGACAGTGGCAGTAGTAATTTGACGAAACCCCAACTCAACTGTGAACTTCCAAACTGCCCCATGGAATTACTTCATCCACCCGAAGATTGTAACATGAACTCTACATTGACCTTCACTCTTCAGATCTGTCGAGTTGAAGATGTTATTGTTCAAGGAAGTATGACTGGATCTGTTATTCATTTTCACTGGGTTAGAGCTGTATCGGTCCACTCTTCTGGGGTAATTAGTGCATCCGGGCTAGGTTGCACTGGTGGGGTTGGTAGAGGAAAGTTTTTCGCAAATGGGCTTGGTGGTGGTGGTGGGCATGGTGGCAAAGGTGGGGATGGGTATTACGATGGAAATTTTATTGATGGTGGTGCTTCCTATGGAGATGCTGATTTGCCTTGTGAACTTGGTAGTGGTAGCGGAAATGATAGCCTAGCTGGTGCAACTGCAGGTGGTGGTATCATTGTAATGGGTTCATTGGAGCGCTCATTGTCAAGTTTGTCTCTTGATGGTTCACTTAGAGCCGATGGAGAAAGCTTTGGAGAAGAATATTTGGAGAAAAATGATAGGATCTTTTCAAATATAGGTCCTGGTGGTGGTTCTGGTGGAACTGTTCTATTGTTTGTTCAAACATTGGAACTTGGTAATTCTTCTACAATCTCAACTGTTGGTGGACATGGTAGTCCTAGTGGTGGTGGTGGAGGAGGTGGTGGGAGGATTCACTTTCACTGGTCAGACATACCAGTTGGGGATGCATATCTACCTATAGCAAGTGCGGGAGGAAGCATCATTACTGAGGGAGGTTTTGGTAGAGGTCATGGTCGGGCTGGACAAAATGGGACTGTTACTGGAAAGGCCTGTCCCAGAGGGCTTTATGGTATCTTTTGTGAGGAATGCCCTATTGGCACCTTCAAGAATGTGAGTGGCTCTGATAGAGCCCTTTGTCATGCTTGCCCATCTTTGGAGCTTCCACATCGTGCCATTTATGTCACGGTTCGAGGTGGTGTTACCAAAACTCCATGTCCATACAAGTGCATTTCTGACAGATATCACATGCCAAAATGTTATACAGCATTTGAAGAGTTGGTTTACACTTTTGGTGGACCATGGTGGTTTGGTCTTATTCTGTTAAGTGTCCTCATCCTGTTAGCTCTCGTGCTTAGCGTTGCACGGATGAAGTATGTTACTGGAGACGAATTACCGACTCCTGTACCTGCTCGACAGGGCTCTCGTTTAGATCATTCCTTCCCTTTCCTGGAATCATTGAATGAGGTTTTGGAAACAAACAGAAATGAGGAATCTCAGAGTCATGTGCATAGGATGTTTTTCATGGGGCCAAATACATTCAGTGAACCTTGGCATCTACCTCATTCCCCTCCAGAACAAGTCACAGATATTGTGTATGAGGCTGCATTCAATAGATTTGTGAACGAGATTAATGCTTTAGCTGCCTATCAGTGGTGGGAAGGATCAGTCTACAGCATTCTTTGTGTTTTTGTTTATCCACTTGCATGGTCATGGTTACAGAGTCGAAGGAAAAAGAAGTTACAACAACTGCGTGAATATGTTCGATCAGAATATGATCATTCTTGCTTGCGTTCTTGTCGTTCACGTGCACTGTATGAAGGACTCAAGGTAGCTGCAACTTCTGATTTAATGCTTGCATACATGGACTTTTACCTCGGTGGAGATGAGAAAAGGGCTGGTCTTCCTCCCCGTCTTCATCAGAGATTTCCATTGTCATTAATTTTTGGAGGAGATGGAAGTTACATGGCTCCTTTCCATCTTTGCAGTGATAACATTCTCACTAGCCTCATGAGTCAGAGTATTCCACCTACCATCTGGTATCGGCTTGTAGCTGGTCTAAACGCTCAACTGCGATTAGTTCGGCAAGGTCACTTAAAGTTGACTTTTGGTCATGTTATCAGCTGGCTTGAAACGCACGCAAACCCTACTCTTAGTGCATATGGCGTACATGTTGCTCTTGCGTGGTTTCAGCCTACAGCTTCTGGGTATAGCCAGTTTGGACTTTTGGTATATGCTATAAACAATGAAAGTGTGCCACCAGCACTGGAGGGTCAAGATGCATTGTCACCGCGGGATCATCTGTCACGTACACCAAGAAATCATTGGGAAAACCCATTTGACCATTTGAGACTCATCGACCATTGGATGTCACAAAAAAGATTTTCTGGAGGAATTTTACTCAGCAAGAGCTTAAGGACGTTTAAAGAGAAGAAAGCTATATGTTATCCCTTTTCTTTCATAGTTTATAATGGCAAACCGGTTGGCCATCAGGATCTTGTCGGTTTGTTCATCTCTATTCTACTCTTGGGAGATTTTAGCCTAGTCTTGCTCAGTCTGCTGCAGCTATATTCCATTTCACTGTTGAACTTTTTCTTGGTCTTATTTATTCTTCCTCTCGGTCTACTATTCCCCTTCCCGGCTGGGATCAGTGCTTTGTTTAGTCATGGACCTAGACGGGCAGCAGGTCTTGCGCGTATATATGCTTTGTGGAATATCACATCCTTGATCAATGTCGTTGTTGCATTTACTTGTGGATTGATTCATTATACGACCCACACGAGGAAGAAGCATTCAAACTTTCAATCCTGGAATTTTAGCATGGATGAAAGTGAATGGTGGGTGCTTCCCTGTGGCCTGGCACTGTGTAAACTCGTGCAGTCGCGTCTCATCGATTGCCATGTGGCGAACCAGGAAATTCAGGATCACTCGTTGTACAGCAAGGACCCTGACGTGTTCTGGCAGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1438

Amino Acids

154.27

Weight (kDa)

6.16

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8003 PF26010 795 - 869 8.1e-36 Domain of unknown function (DUF8003)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3813
AarI CACCTGC 2 cut(s) 959, 1979
AasI GACNNNNNNGTC 1 cut(s) 2220
Acc36I ACCTGC 4 cut(s) 959, 1979, 2761, 4019
AccB1I GGYRCC 2 cut(s) 497, 2444
AccBSI CCGCTC 1 cut(s) 184
AccI GTMKAC 2 cut(s) 3015, 3967
AccII CGCG 3 cut(s) 3637, 4039, 4228
AcoI YGGCCR 1 cut(s) 3829
AcuI CTGAAG 2 cut(s) 1254, 1658
AcvI CACGTG 1 cut(s) 3152
AcyI GRCGYC 1 cut(s) 1400
AdeI CACNNNGTG 1 cut(s) 2516
AfaI GTAC 5 cut(s) 999, 2751, 3513, 3655, 4280
AfeI AGCGCT 1 cut(s) 2024
AflII CTTAAG 1 cut(s) 3757
AflIII ACRYGT 2 cut(s) 3514, 4296
AgeI ACCGGT 1 cut(s) 3823
AhdI GACNNNNNGTC 2 cut(s) 1013, 3453
AhlI ACTAGT 2 cut(s) 491, 659
AjiI CACGTC 1 cut(s) 4297
AjnI CCWGG 9 cut(s) 192, 1465, 1528, 1551, 2128, 2793, 4151, 4200, 4254
AjuI GAANNNNNNNTTGG 6 cut(s) 2424, 2456, 2798, 2830, 3923, 3955
Alw21I GWGCWC 2 cut(s) 478, 3157
Alw44I GTGCAC 2 cut(s) 474, 3153
AlwNI CAGNNNCTG 2 cut(s) 974, 3548
Aor51HI AGCGCT 1 cut(s) 2024
AoxI GGCC 6 cut(s) 1463, 1547, 2394, 2873, 3829, 4198
ApaI GGGCCC 1 cut(s) 1551
ApaLI GTGCAC 2 cut(s) 474, 3153
ApeKI GCWGC 9 cut(s) 321, 795, 974, 2948, 2987, 3179, 3904, 3907, 4025
AseI ATTAAT 2 cut(s) 2976, 3284
AsiGI ACCGGT 1 cut(s) 3823
Asp700I GAANNNNTTC 5 cut(s) 886, 1239, 1623, 3105, 3935
AspLEI GCGC 4 cut(s) 83, 487, 2025, 4039
AsuC2I CCSGG 6 cut(s) 306, 400, 482, 503, 1789, 3983
AsuHPI GGTGA 2 cut(s) 770, 3624
AsuII TTCGAA 3 cut(s) 117, 422, 1282
AvaII GGWCC 6 cut(s) 1526, 1759, 2126, 2642, 4013, 4288
BaeGI GKGCMC 3 cut(s) 478, 1551, 3157
BaeI ACNNNNGTAYC 2 cut(s) 2733, 2766
BalI TGGCCA 1 cut(s) 3831
BamHI GGATCC 1 cut(s) 598
BanI GGYRCC 2 cut(s) 497, 2444
BanII GRGCYC 4 cut(s) 1279, 1551, 2479, 2768
BarI GAAGNNNNNNTAC 2 cut(s) 2314, 2346
BauI CACGAG 4 cut(s) 187, 2693, 4123, 4217
BbrPI CACGTG 1 cut(s) 3152
BbsI GAAGAC 2 cut(s) 3239, 3251
Bbv12I GWGCWC 2 cut(s) 478, 3157
BbvI GCAGC 9 cut(s) 308, 807, 961, 2935, 2974, 3166, 3891, 3919, 4037
BceAI ACGGC 2 cut(s) 477, 1122
BcgI CGANNNNNNTGC 2 cut(s) 2960, 2994
BciT130I CCWGG 9 cut(s) 194, 1467, 1530, 1553, 2130, 2795, 4153, 4202, 4256
BclI TGATCA 2 cut(s) 3121, 4071
BcnI CCSGG 6 cut(s) 306, 400, 482, 503, 1789, 3983
BcuI ACTAGT 2 cut(s) 491, 659
BfmI CTRYAG 5 cut(s) 1986, 2304, 3016, 3540, 3905
BfoI RGCGCY 1 cut(s) 2026
BfrI CTTAAG 1 cut(s) 3757
BfuAI ACCTGC 4 cut(s) 959, 1979, 2761, 4019
BglI GCCNNNNNGGC 1 cut(s) 2442
BglII AGATCT 1 cut(s) 1677
BlpI GCTNAGC 1 cut(s) 2699
Bme18I GGWCC 6 cut(s) 1526, 1759, 2126, 2642, 4013, 4288
BmeRI GACNNNNNGTC 2 cut(s) 1013, 3453
BmgBI CACGTC 1 cut(s) 4297
BmrI ACTGGG 1 cut(s) 1750
BmsI GCATC 7 cut(s) 982, 1793, 1915, 2281, 2334, 2908, 3613
BmuI ACTGGG 1 cut(s) 1750
BpiI GAAGAC 2 cut(s) 3239, 3251
BpmI CTGGAG 5 cut(s) 888, 2748, 2901, 3632, 3753
Bpu1102I GCTNAGC 1 cut(s) 2699
Bpu14I TTCGAA 3 cut(s) 117, 422, 1282
BpuEI CTTGAG 2 cut(s) 1388, 3155
BpuMI CCSGG 6 cut(s) 306, 400, 482, 503, 1789, 3983
Bsa29I ATCGAT 1 cut(s) 4236
BsaAI YACGTR 2 cut(s) 3152, 3653
BsaBI GATNNNNATC 1 cut(s) 2295
BsaHI GRCGYC 1 cut(s) 1400
BsaI GGTCTC 3 cut(s) 206, 222, 1464
BsaWI WCCGGW 1 cut(s) 3823
BsaXI ACNNNNNCTCC 2 cut(s) 2539, 2569
Bse118I RCCGGY 1 cut(s) 3823
Bse8I GATNNNNATC 1 cut(s) 2295
BseBI CCWGG 9 cut(s) 194, 1467, 1530, 1553, 2130, 2795, 4153, 4202, 4256
BseCI ATCGAT 1 cut(s) 4236
BseJI GATNNNNATC 1 cut(s) 2295
BseMII CTCAG 5 cut(s) 1405, 2325, 2855, 3760, 3910
BseRI GAGGAG 4 cut(s) 885, 888, 2256, 3309
BseSI GKGCMC 3 cut(s) 478, 1551, 3157
BseXI GCAGC 9 cut(s) 308, 807, 961, 2935, 2974, 3166, 3891, 3919, 4037
BseYI CCCAGC 1 cut(s) 3986
BsgI GTGCAG 3 cut(s) 864, 1197, 4241
Bsh1236I CGCG 3 cut(s) 3637, 4039, 4228
BshFI GGCC 6 cut(s) 1465, 1549, 2396, 2875, 3831, 4200
BshNI GGYRCC 2 cut(s) 497, 2444
BshTI ACCGGT 1 cut(s) 3823
BshVI ATCGAT 1 cut(s) 4236
BsiHKAI GWGCWC 2 cut(s) 478, 3157
BsiSI CCGG 7 cut(s) 306, 399, 482, 503, 1788, 3824, 3983
BslFI GGGAC 3 cut(s) 143, 2385, 2392
BsmBI CGTCTC 2 cut(s) 2724, 4235
BsmFI GGGAC 3 cut(s) 143, 2385, 2392
BsmI GAATGC 5 cut(s) 430, 2438, 2951, 3021, 4135
BsnI GGCC 6 cut(s) 1465, 1549, 2396, 2875, 3831, 4200
Bso31I GGTCTC 3 cut(s) 206, 222, 1464
Bsp119I TTCGAA 3 cut(s) 117, 422, 1282
Bsp120I GGGCCC 1 cut(s) 1547
Bsp1286I GDGCHC 6 cut(s) 478, 1279, 1551, 2479, 2768, 3157
Bsp1407I TGTACA 1 cut(s) 4278
Bsp1720I GCTNAGC 1 cut(s) 2699
Bsp19I CCATGG 2 cut(s) 1614, 2645
BspANI GGCC 6 cut(s) 1465, 1549, 2396, 2875, 3831, 4200
BspCNI CTCAG 5 cut(s) 1404, 2326, 2854, 3759, 3909
BspDI ATCGAT 1 cut(s) 4236
BspFNI CGCG 3 cut(s) 3637, 4039, 4228
BspHI TCATGA 1 cut(s) 3354
BspMAI CTGCAG 2 cut(s) 1990, 3909
BspMI ACCTGC 4 cut(s) 959, 1979, 2761, 4019
BspQI GCTCTTC 2 cut(s) 1230, 1284
BspT104I TTCGAA 3 cut(s) 117, 422, 1282
BspT107I GGYRCC 2 cut(s) 497, 2444
BspTI CTTAAG 1 cut(s) 3757
BspTNI GGTCTC 3 cut(s) 206, 222, 1464
BsrBI CCGCTC 1 cut(s) 184
BsrFI RCCGGY 1 cut(s) 3823
BsrGI TGTACA 1 cut(s) 4278
BssAI RCCGGY 1 cut(s) 3823
BssNI GRCGYC 1 cut(s) 1400
BssSI CACGAG 4 cut(s) 187, 2693, 4123, 4217
BssT1I CCWWGG 4 cut(s) 1110, 1614, 2645, 2894
Bst2BI CACGAG 4 cut(s) 187, 2693, 4123, 4217
Bst2UI CCWGG 9 cut(s) 194, 1467, 1530, 1553, 2130, 2795, 4153, 4202, 4256
Bst4CI ACNGT 9 cut(s) 1559, 1595, 2152, 2206, 2383, 2533, 3159, 3927, 4209
Bst6I CTCTTC 7 cut(s) 53, 877, 1230, 1284, 1677, 1771, 2613
BstACI GRCGYC 1 cut(s) 1400
BstAFI CTTAAG 1 cut(s) 3757
BstAPI GCANNNNNTGC 3 cut(s) 974, 1205, 4034
BstAUI TGTACA 1 cut(s) 4278
BstBAI YACGTR 2 cut(s) 3152, 3653
BstBI TTCGAA 3 cut(s) 117, 422, 1282
BstC8I GCNNGC 9 cut(s) 236, 430, 908, 1375, 2491, 3134, 3201, 3470, 3483
BstDEI CTNAG 9 cut(s) 731, 1391, 2057, 2334, 2699, 2841, 3497, 3746, 3896
BstDSI CCRYGG 3 cut(s) 1614, 2645, 3635
BstFNI CGCG 3 cut(s) 3637, 4039, 4228
BstH2I RGCGCY 1 cut(s) 2026
BstHHI GCGC 4 cut(s) 83, 487, 2025, 4039
BstNI CCWGG 9 cut(s) 194, 1467, 1530, 1553, 2130, 2795, 4153, 4202, 4256
BstNSI RCATGY 2 cut(s) 2596, 3518
BstSFI CTRYAG 5 cut(s) 1986, 2304, 3016, 3540, 3905
BstSLI GKGCMC 3 cut(s) 478, 1551, 3157
BstUI CGCG 3 cut(s) 3637, 4039, 4228
BstV1I GCAGC 9 cut(s) 308, 807, 961, 2935, 2974, 3166, 3891, 3919, 4037
BstV2I GAAGAC 2 cut(s) 3239, 3251
BstX2I RGATCY 6 cut(s) 598, 1532, 1677, 1721, 2109, 3838
BstXI CCANNNNNNTGG 3 cut(s) 1117, 1769, 3051
BstYI RGATCY 6 cut(s) 598, 1532, 1677, 1721, 2109, 3838
Bsu15I ATCGAT 1 cut(s) 4236
BsuRI GGCC 6 cut(s) 1465, 1549, 2396, 2875, 3831, 4200
BsuTUI ATCGAT 1 cut(s) 4236
BtgI CCRYGG 3 cut(s) 1614, 2645, 3635
BtrI CACGTC 1 cut(s) 4297
BtsI GCAGTG 2 cut(s) 852, 3337
BveI ACCTGC 4 cut(s) 959, 1979, 2761, 4019
Cac8I GCNNGC 9 cut(s) 236, 430, 908, 1375, 2491, 3134, 3201, 3470, 3483
CaiI CAGNNNCTG 2 cut(s) 974, 3548
CciI TCATGA 1 cut(s) 3354
CfoI GCGC 4 cut(s) 83, 487, 2025, 4039
Cfr10I RCCGGY 1 cut(s) 3823
Cfr42I CCGCGG 1 cut(s) 3638
ClaI ATCGAT 1 cut(s) 4236
CseI GACGC 2 cut(s) 1389, 4217
Csp6I GTAC 5 cut(s) 998, 2750, 3512, 3654, 4279
CspAI ACCGGT 1 cut(s) 3823
CviQI GTAC 5 cut(s) 998, 2750, 3512, 3654, 4279
DdeI CTNAG 9 cut(s) 731, 1391, 2057, 2334, 2699, 2841, 3497, 3746, 3896
DraI TTTAAA 1 cut(s) 3769
DraIII CACNNNGTG 1 cut(s) 2516
DrdI GACNNNNNNGTC 1 cut(s) 2220
DriI GACNNNNNGTC 2 cut(s) 1013, 3453
DseDI GACNNNNNNGTC 1 cut(s) 2220
EaeI YGGCCR 1 cut(s) 3829
Eam1104I CTCTTC 7 cut(s) 53, 877, 1230, 1284, 1677, 1771, 2613
Eam1105I GACNNNNNGTC 2 cut(s) 1013, 3453
EarI CTCTTC 7 cut(s) 53, 877, 1230, 1284, 1677, 1771, 2613
EciI GGCGGA 1 cut(s) 150
Eco130I CCWWGG 4 cut(s) 1110, 1614, 2645, 2894
Eco147I AGGCCT 1 cut(s) 2396
Eco24I GRGCYC 4 cut(s) 1279, 1551, 2479, 2768
Eco31I GGTCTC 3 cut(s) 206, 222, 1464
Eco32I GATATC 2 cut(s) 925, 2588
Eco47I GGWCC 6 cut(s) 1526, 1759, 2126, 2642, 4013, 4288
Eco47III AGCGCT 1 cut(s) 2024
Eco57I CTGAAG 2 cut(s) 1254, 1658
Eco72I CACGTG 1 cut(s) 3152
EcoO109I RGGNCCY 4 cut(s) 1547, 1548, 2126, 4288
EcoRI GAATTC 2 cut(s) 241, 1312
EcoRII CCWGG 9 cut(s) 192, 1465, 1528, 1551, 2128, 2793, 4151, 4200, 4254
EcoRV GATATC 2 cut(s) 925, 2588
EcoT14I CCWWGG 4 cut(s) 1110, 1614, 2645, 2894
EcoT22I ATGCAT 2 cut(s) 2296, 3628
EcoT38I GRGCYC 4 cut(s) 1279, 1551, 2479, 2768
ErhI CCWWGG 4 cut(s) 1110, 1614, 2645, 2894
Esp3I CGTCTC 2 cut(s) 2724, 4235
FaqI GGGAC 3 cut(s) 143, 2385, 2392
FauI CCCGC 4 cut(s) 189, 970, 2308, 3630
FauNDI CATATG 1 cut(s) 3505
FbaI TGATCA 2 cut(s) 3121, 4071
FblI GTMKAC 2 cut(s) 3015, 3967
FriOI GRGCYC 4 cut(s) 1279, 1551, 2479, 2768
GlaI GCGC 4 cut(s) 82, 486, 2024, 4038
GsaI CCCAGC 1 cut(s) 3990
GsuI CTGGAG 5 cut(s) 888, 2748, 2901, 3632, 3753
HaeII RGCGCY 1 cut(s) 2026
HaeIII GGCC 6 cut(s) 1465, 1549, 2396, 2875, 3831, 4200
HapII CCGG 7 cut(s) 306, 399, 482, 503, 1788, 3824, 3983
HgaI GACGC 2 cut(s) 1389, 4217
HhaI GCGC 4 cut(s) 83, 487, 2025, 4039
Hin1I GRCGYC 1 cut(s) 1400
Hin6I GCGC 4 cut(s) 81, 485, 2023, 4037
HinP1I GCGC 4 cut(s) 81, 485, 2023, 4037
HincII GTYRAC 3 cut(s) 352, 1093, 3447
HindII GTYRAC 3 cut(s) 352, 1093, 3447
HindIII AAGCTT 2 cut(s) 247, 2074
HpaI GTTAAC 1 cut(s) 352
HpaII CCGG 7 cut(s) 306, 399, 482, 503, 1788, 3824, 3983
HphI GGTGA 2 cut(s) 770, 3624
HpyCH4III ACNGT 9 cut(s) 1559, 1595, 2152, 2206, 2383, 2533, 3159, 3927, 4209
HpyCH4IV ACGT 4 cut(s) 3151, 3652, 3764, 4296
HpyF3I CTNAG 9 cut(s) 731, 1391, 2057, 2334, 2699, 2841, 3497, 3746, 3896
HpySE526I ACGT 4 cut(s) 3151, 3652, 3764, 4296
Hsp92I GRCGYC 1 cut(s) 1400
HspAI GCGC 4 cut(s) 81, 485, 2023, 4037
Ksp22I TGATCA 2 cut(s) 3121, 4071
KspAI GTTAAC 1 cut(s) 352
KspI CCGCGG 1 cut(s) 3638
LguI GCTCTTC 2 cut(s) 1230, 1284
LmnI GCTCC 6 cut(s) 288, 579, 1134, 2020, 2503, 3319
Lsp1109I GCAGC 9 cut(s) 308, 807, 961, 2935, 2974, 3166, 3891, 3919, 4037
LweI GCATC 7 cut(s) 982, 1793, 1915, 2281, 2334, 2908, 3613
MaeII ACGT 4 cut(s) 3151, 3652, 3764, 4296
MbiI CCGCTC 1 cut(s) 184
MfeI CAATTG 2 cut(s) 723, 1347
MflI RGATCY 6 cut(s) 598, 1532, 1677, 1721, 2109, 3838
MhlI GDGCHC 6 cut(s) 478, 1279, 1551, 2479, 2768, 3157
MlsI TGGCCA 1 cut(s) 3831
MluNI TGGCCA 1 cut(s) 3831
MlyI GAGTC 7 cut(s) 166, 2737, 2854, 3076, 3162, 3367, 3690
MmeI TCCRAC 2 cut(s) 600, 687
Mox20I TGGCCA 1 cut(s) 3831
Mph1103I ATGCAT 2 cut(s) 2296, 3628
MroXI GAANNNNTTC 5 cut(s) 886, 1239, 1623, 3105, 3935
MscI TGGCCA 1 cut(s) 3831
MslI CAYNNNNRTG 5 cut(s) 1416, 2006, 2569, 3049, 3055
Msp20I TGGCCA 1 cut(s) 3831
MspA1I CMGCKG 2 cut(s) 3468, 3637
MspCI CTTAAG 1 cut(s) 3757
MspI CCGG 7 cut(s) 306, 399, 482, 503, 1788, 3824, 3983
MunI CAATTG 2 cut(s) 723, 1347
Mva1269I GAATGC 5 cut(s) 430, 2438, 2951, 3021, 4135
MvaI CCWGG 9 cut(s) 194, 1467, 1530, 1553, 2130, 2795, 4153, 4202, 4256
MvnI CGCG 3 cut(s) 3637, 4039, 4228
NciI CCSGG 6 cut(s) 306, 400, 482, 503, 1789, 3983
NcoI CCATGG 2 cut(s) 1614, 2645
NdeI CATATG 1 cut(s) 3505
NmuCI GTSAC 6 cut(s) 2527, 2926, 3434, 3630, 3648, 3714
NsiI ATGCAT 2 cut(s) 2296, 3628
NspI RCATGY 2 cut(s) 2596, 3518
NspV TTCGAA 3 cut(s) 117, 422, 1282
PagI TCATGA 1 cut(s) 3354
PaqCI CACCTGC 2 cut(s) 959, 1979
PceI AGGCCT 1 cut(s) 2396
PciI ACATGT 1 cut(s) 3514
PciSI GCTCTTC 2 cut(s) 1230, 1284
PcsI WCGNNNNNNNCGW 3 cut(s) 152, 2700, 4121
PctI GAATGC 5 cut(s) 430, 2438, 2951, 3021, 4135
PdmI GAANNNNTTC 5 cut(s) 886, 1239, 1623, 3105, 3935
PfoI TCCNGGA 2 cut(s) 2793, 4151
PinAI ACCGGT 1 cut(s) 3823
PleI GAGTC 7 cut(s) 165, 2737, 2853, 3075, 3162, 3366, 3690
PmaCI CACGTG 1 cut(s) 3152
PmlI CACGTG 1 cut(s) 3152
PpsI GAGTC 7 cut(s) 165, 2737, 2853, 3075, 3162, 3366, 3690
Ppu21I YACGTR 2 cut(s) 3152, 3653
PpuMI RGGWCCY 2 cut(s) 2126, 4288
PscI ACATGT 1 cut(s) 3514
PshBI ATTAAT 2 cut(s) 2976, 3284
PsiI TTATAA 1 cut(s) 3813
Psp5II RGGWCCY 2 cut(s) 2126, 4288
Psp6I CCWGG 9 cut(s) 192, 1465, 1528, 1551, 2128, 2793, 4151, 4200, 4254
PspCI CACGTG 1 cut(s) 3152
PspFI CCCAGC 1 cut(s) 3986
PspGI CCWGG 9 cut(s) 192, 1465, 1528, 1551, 2128, 2793, 4151, 4200, 4254
PspOMI GGGCCC 1 cut(s) 1547
PspPPI RGGWCCY 2 cut(s) 2126, 4288
PstI CTGCAG 2 cut(s) 1990, 3909
PstNI CAGNNNCTG 2 cut(s) 974, 3548
PsuI RGATCY 6 cut(s) 598, 1532, 1677, 1721, 2109, 3838
PvuII CAGCTG 1 cut(s) 3468
RsaI GTAC 5 cut(s) 999, 2751, 3513, 3655, 4280
RsaNI GTAC 5 cut(s) 998, 2750, 3512, 3654, 4279
RseI CAYNNNNRTG 5 cut(s) 1416, 2006, 2569, 3049, 3055
SacII CCGCGG 1 cut(s) 3638
SapI GCTCTTC 2 cut(s) 1230, 1284
SchI GAGTC 7 cut(s) 166, 2737, 2854, 3076, 3162, 3367, 3690
SduI GDGCHC 6 cut(s) 478, 1279, 1551, 2479, 2768, 3157
SfaNI GCATC 7 cut(s) 982, 1793, 1915, 2281, 2334, 2908, 3613
SfcI CTRYAG 5 cut(s) 1986, 2304, 3016, 3540, 3905
Sfr303I CCGCGG 1 cut(s) 3638
SfuI TTCGAA 3 cut(s) 117, 422, 1282
SgrBI CCGCGG 1 cut(s) 3638
SinI GGWCC 6 cut(s) 1526, 1759, 2126, 2642, 4013, 4288
SmiMI CAYNNNNRTG 5 cut(s) 1416, 2006, 2569, 3049, 3055
SmlI CTYRAG 3 cut(s) 1367, 3170, 3757
SmoI CTYRAG 3 cut(s) 1367, 3170, 3757
SpeI ACTAGT 2 cut(s) 491, 659
SseBI AGGCCT 1 cut(s) 2396
SspI AATATT 1 cut(s) 2090
StuI AGGCCT 1 cut(s) 2396
StyI CCWWGG 4 cut(s) 1110, 1614, 2645, 2894
TaaI ACNGT 9 cut(s) 1559, 1595, 2152, 2206, 2383, 2533, 3159, 3927, 4209
TaiI ACGT 4 cut(s) 3154, 3655, 3767, 4299
TaqII GACCGA 2 cut(s) 1747, 3953
TatI WGTACW 1 cut(s) 4278
TauI GCSGC 3 cut(s) 151, 166, 169
TseFI GTSAC 6 cut(s) 2527, 2926, 3434, 3630, 3648, 3714
TseI GCWGC 9 cut(s) 321, 795, 974, 2948, 2987, 3179, 3904, 3907, 4025
Tsp45I GTSAC 6 cut(s) 2527, 2926, 3434, 3630, 3648, 3714
TspGWI ACGGA 4 cut(s) 222, 591, 1452, 2725
Vha464I CTTAAG 1 cut(s) 3757
VneI GTGCAC 2 cut(s) 474, 3153
VpaK11BI GGWCC 6 cut(s) 1526, 1759, 2126, 2642, 4013, 4288
VspI ATTAAT 2 cut(s) 2976, 3284
XceI RCATGY 2 cut(s) 2596, 3518
XcmI CCANNNNNNNNNTGG 3 cut(s) 585, 3378, 3666
XmiI GTMKAC 2 cut(s) 3015, 3967
XmnI GAANNNNTTC 5 cut(s) 886, 1239, 1623, 3105, 3935
Zsp2I ATGCAT 2 cut(s) 2296, 3628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.