FvH4_2g35120

glycine-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002500
Physical Location & Seq
Forward (+)
25952910 .. 25962812
9903 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35120.t3

Sequence Viewer

Length: 4341 bp
ATGGCTCGCTTTCTTCATTTCGCTTTCTGCTTCATCCTCGTAGCCCTAACCACAAACCCTAGCCTCCTCCGAGCAGATGACGACGAGTTGAACGATTCCGATTCCGAATCCAATCTCTTCCACCAGGACTACTCGCCGCCGGCTCCGCCACCGCCGCCGCCGCTCCCGCCGTCGGTGTCCTGCACCGACGACCTCGGCGGCGTCGGGTCGCTGGACACCACGTGTCAGATAGTCTCTGATGCGAACCTCACCGGAGACGTGTATATACATGGGAGAGGAAACTTTTATATCCTGCCCGGGGTGAGATTCCATTGTGCGACTCCTGGTTGCTCCATAATCGTTAACATTACTGGTAATTTTAGCTTAGGTAACTCGTCGTCGATTTTCGCCGGAGATTTTGAGCTCGTGGCGTTCAATGCAAGCTTCCACAACGGCTCGGCGGTGAACACTACCGCTTTGGCGGGGAAGCCGCCGCCGCAGACGAGCGGGACTCCGCAGGGGATAGATGGCGCCGGCGGGGGGCATGGAGGCCGCGGCGCGTGTTGTTTGGTGGACCGGACGAAGCTTCCGGAAGACGTGTGGGGCGGCGACGCCTACTCATGGTCGTCGCTGCAGAAGCCGTGTAGTTTTGGGAGCCGAGGCGGTTCGACGAGCAAGGAAGTGGATTATGGAGGCTTAGGGGGAGGGATAGTGAAGCTGGAGATTAGGAATTATCTGGAGGTGAATGGAAGTGCTTTGGCTGATGGAGGTGATGGAGGTTCGAAAGGCGGAGGTGGTTCTGGTGGCAGCATCTACATTAAGGCTCAGAGAATGACTGGTAGTGGCAGGATAAGTGCTTGTGGTGGTAATGGTTATGCTGGTGGCGGTGGTGGAAGAGTGTCAATTGATGTTTTTAGCAGGCATGATTATCCTGCAGTATTTGTGCATGGAGGAAGTAGCAATGCTTGCCCAGAAAATGCAGGTGGTGCTGGTACTTTATATGACGCAGTTCCTCGAAGCCTTATTATTAGCAATCATAACAAGTCAACAGATACAGAAACGCTTCTTTTAGAGTTCCCTTATCAGCCTCTTTGGACAAATGTATATGTTCGAAACAAAGCTAGGGCTACTGTCCCTCTGCTTTGGAGTCGAGTCCAGGTGCAAGGACAGATAAGTCTTCTATGTGACGGTGTGCTAAGCTTTGGACTTCAACATTATGCTTCATCAGAGTTTGAGTTATTGGCTGAAGAATTGCTAATGAGCGATTCTGTAATAAAGGTGTATGGGGCCTTGCGCATGTCTGTAAAAATGTTCTTGATGTGGAATTCCAAGATGCTCATAGATGGTGGTGGAGAAGAAGCTGTTGAAACATCCTTACTGGAGGCTAGTAACTTGATTGTTCTCAGGGAGTCATCTGTGATACACTCTAATGCAAATTTGGGAGTTCATGGACAAGGGTTACTGAACCTATCAGGAGCAGGAGATTGGATTCAAGCACAACGTCTGGTTTTGTCACTATTCTACAGTATTCATGTTGGGCCTGGATCTGTTTTGCGTGGCCCCTTGGAGAACGCAACATCTGATTCTGTAACACCAAAGCTCTACTGTGAAAATGAAGATTGCCCTGTTGAGCTACTTCATCCACCTGAAGATTGCAATGTGAACTCCTCACTGTCCTTTACACTCCAGATATGCCGGGTTGAGGATATCAACATTTTAGGCCACATAAAAGGATCCGTTGTTCATTTTCACAGGGCAAGGACTATTGCTGTCCACTCTACTGGAATGATAAGTGCATCAGGAATGGGCTGCACTGGTGGTATTGGGAGTGGCAACGTTCTAAGCAATGGAATTGGCAGTGGTGGTGGGCATGGCGGTAAAGGTGGGGCTGCATGTAGTAATAATGGTAGTTGTGTTGAAGGTGGAATTTCATATGGGAATGCAAAGTTGCCTTGTGAACTTGGTAGTGGAAGTGGATATGACATTACAGCTGGTTCAACTGCTGGTGGTGGTATTATTGTAATGGGTTCTGTAGAACATCCATTGTCAAGTTTGACTGTTGAAGGCTTGGTGACATCTGATGGTGAAAGTTTTGAAAGAAACACAGTAATTGGAAAGCTTCCTATTGCTGATAGTTTAAGTGGACATCCTGGGGGTGGGTCTGGTGGAACAATACTTCTCTTCTTGCGAACATTAACTCTTGGTGAATCTGCCAATCTTTCAACTGTTGGTGGATATGGAAGCTCCAATGGGTCAGGTGCCGGTGGTGGTGGAAGAATTCATTTTCATTGGTCAGACATTCCCACTGGAGATGTGTATCAGCCAATTGCGAGTGTTGAAGGAAACATCCATGCAAGGGGAGGGAGAGGCAGAGACCAGGGTGGTGCTGGAGAGGATGGGACAGTGACGGGAAAAGCATGCCCAAAGGGGTTGTATGGGACCTTTTGTGAGGAATGTCCAGCTGGCACTTATAAGAATGTCATTGGGTCTGATGGGGCACTTTGTCAAAATTGTCCTGCTGATCAGCTTCCCCAACGTGCAGTTTATATTTCTGTCCGAGGTGGAGTTGCCGAGACACCGTGTCCTTACAAATGCATTTCAGATAGATATCACTTGCCAAACTGTTATACGGCTCTTGAAGAGTTGATCTACACATTTGGTGGGCCTTGGCTATTTGGTCTTCTTCTGATTGGTCTCCTCATACTATTAGCTTTGGTGCTAAGTGTTGCACGTATGAAATTTGTTGGGGTTGATGAAGCACCAGGCCCAGCTCCCACTCAGCATGGCTCACAAATAGACCATTCTTTTCCATTCCTTGAATCTTTGAACGAGGTTTTGGAAACAAATAGAGCTGAGGAGTCCCATAGCCATGTGCACAGAATGTATTTTATGGGTCCAAATACATTCAGTGAACCTTGGCATCTGCCTCGCACTCCTCCAGAACAAATAAAGGAAATTGTATATGAAGGTCCATTCAATACATTTGTTGCTGAGATCAATGCCATAACCACTTATCAGTGGTGGGAGGGAGCAATGTACATCATTCTTGCTGTTCTTGCATACCCCCTTGCCATCTCATGGCAACAGTGGCGCCGAAGATTGAAATTGCAACGCCTGCGTGAATTTGTTCGATCAGAATATGATCATGCCTGTTTACGGTCTTGTCGTTCACGTGCTCTATATGAAGGAATTAAGGTAAATGCAACCGCTGATTTAATGCTAGCTTATGTGGACTTCTTCCTTGGTGGGGATGAAAAGAGAACTGATCTTCCTCCTCGTTTACATCAAAGATTTCCTATGTCATTACCTTTTGGAGGTGATGGAAGTTACATGGCTCCTTTCTGTCTTCACAGTGATAATATTGTCACAAGTCTCATGAGTCAGTCGATCCCACCTACCACATGGTACCGAATGGTGGCTGGTTTGAATGCGCAATTACGCTTAGTTTGCCGTGGAAGGCTAAGAGTTACACTCCTACCTGTCCTTACGTGGCTTGAAAGCCATGCCAATCCGGCTCTGAGGAATTATGGTGTACATGTTGATCTTGCGTGGTTTCAGGCTACTGCTTGTGGTTATCGCCATTATGGACTTGTGGTGTATGCTCGTGAAGAAGACAACGTTCCTGTTTCTGTTGGGAGCATTGATGGAGCAATTGGAAATGAAGAGTCACGTGCAAGTAGCATTTACAAAGAAGATTCCTCTGTTCATTTGATAGAAGAACCACTAATAAGCCATTCCCACAGAAGCAATATGAATCGTGTGAGGCGAAGGAGAGCATGTGGAGGGATTGTGGATGCCGACAATTTACAAATGCTCGAGGACAAGAGAGATATATTTTATCCTCTCTCTTTTATGCTTCATAATACTAAACCTGTTGGACACCAGGATCTTGTTGGTTTAGTCATCTCAATGCTTCTTTTAGGAGATTTTAGTTTAGTTTTGCTCACATTACTCCAGCTGTATTCGTTTTCACTAGTGGACGTCTTTCTGGTTCTGTTTTTTTTACCACTTGGCTTTCTTCTCCCATTTCCTGCTGGCATCAATGCTCTATTCAGTCATGGACCCAGACGTTCTGCCGGCCTCGCACGTATACATGCTCTGTGGAATGTTACATCCTTAATTAATGTTGTGGTTGCTTTCATTTGTGGATATGCTCACTTTAACAGTCAAGCGTCAAGTAAAAAACATCCATTACAACCCTGGAATATAAGCATGGATGACAGTGAATGGTGGATTTTTCCTGCGGGTCTGGTTGTGTGTAAAATTTTGCAATCACAGCTAATCAATTGGCATGTTGCCAACCTGGAGATTCAAGACCGCTCCTTGTACAGCAATGATTTTGAGCTGTTCTGGCAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1447

Amino Acids

155.22

Weight (kDa)

5.81

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8003 PF26010 383 - 457 1.4e-35 Domain of unknown function (DUF8003)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2451
AarI CACCTGC 1 cut(s) 950
AasI GACNNNNNNGTC 1 cut(s) 1152
AatII GACGTC 1 cut(s) 3963
Acc16I TGCGCA 2 cut(s) 1274, 3411
Acc36I ACCTGC 1 cut(s) 950
Acc65I GGTACC 1 cut(s) 3384
AccB1I GGYRCC 4 cut(s) 509, 2237, 3069, 3384
AccB7I CCANNNNNTGG 1 cut(s) 3057
AccBSI CCGCTC 3 cut(s) 163, 486, 4299
AccI GTMKAC 1 cut(s) 4069
AccII CGCG 2 cut(s) 534, 539
AccIII TCCGGA 1 cut(s) 568
AclI AACGTT 2 cut(s) 1815, 3597
AclWI GGATC 5 cut(s) 1531, 1707, 1720, 3361, 3873
AcsI RAATTY 7 cut(s) 1303, 1414, 1905, 2256, 2717, 3101, 4242
AcuI CTGAAG 2 cut(s) 1245, 1647
AcvI CACGTG 3 cut(s) 222, 3152, 3650
AcyI GRCGYC 5 cut(s) 201, 510, 591, 3070, 3960
AdeI CACNNNGTG 1 cut(s) 222
AfaI GTAC 5 cut(s) 973, 3017, 3386, 3513, 4307
AflIII ACRYGT 4 cut(s) 221, 258, 576, 3514
AhdI GACNNNNNGTC 1 cut(s) 2559
AhlI ACTAGT 1 cut(s) 3952
AjiI CACGTC 2 cut(s) 259, 577
AjuI GAANNNNNNNTTGG 2 cut(s) 2798, 2830
AloI GAACNNNNNNTCC 4 cut(s) 1704, 1736, 3232, 3264
Alw21I GWGCWC 3 cut(s) 405, 2856, 3157
Alw26I GTCTC 6 cut(s) 238, 249, 2346, 2546, 2678, 3356
Alw44I GTGCAC 1 cut(s) 2852
AlwI GGATC 5 cut(s) 1531, 1707, 1720, 3361, 3873
Ama87I CYCGRG 2 cut(s) 296, 3794
Aor13HI TCCGGA 1 cut(s) 568
AoxI GGCC 8 cut(s) 529, 1266, 1517, 1537, 1699, 2642, 2743, 4057
ApaLI GTGCAC 1 cut(s) 2852
ApeKI GCWGC 4 cut(s) 610, 786, 1788, 1868
ApoI RAATTY 7 cut(s) 1303, 1414, 1905, 2256, 2717, 3101, 4242
AseI ATTAAT 1 cut(s) 4101
Asp700I GAANNNNTTC 2 cut(s) 1041, 3105
Asp718I GGTACC 1 cut(s) 3384
AspLEI GCGC 5 cut(s) 512, 539, 1275, 3072, 3412
AsuC2I CCSGG 3 cut(s) 297, 298, 1676
AsuHPI GGTGA 9 cut(s) 241, 313, 454, 733, 761, 2062, 2075, 2195, 3308
AsuII TTCGAA 2 cut(s) 761, 1090
AsuNHI GCTAGC 1 cut(s) 3199
AvaI CYCGRG 2 cut(s) 296, 3794
AvaII GGWCC 5 cut(s) 553, 2418, 2873, 2948, 4040
BaeGI GKGCMC 2 cut(s) 2479, 2856
BamHI GGATCC 1 cut(s) 1712
BanI GGYRCC 4 cut(s) 509, 2237, 3069, 3384
BanII GRGCYC 1 cut(s) 405
BauI CACGAG 2 cut(s) 404, 3582
BbrPI CACGTG 3 cut(s) 222, 3152, 3650
BbsI GAAGAC 5 cut(s) 579, 1148, 2651, 3317, 3597
Bbv12I GWGCWC 3 cut(s) 405, 2856, 3157
BbvCI CCTCAGC 1 cut(s) 2832
BbvI GCAGC 4 cut(s) 597, 798, 1775, 1855
BceAI ACGGC 5 cut(s) 154, 448, 604, 2625, 3414
BcgI CGANNNNNNTGC 2 cut(s) 2888, 2922
BclI TGATCA 2 cut(s) 2500, 3121
BcnI CCSGG 3 cut(s) 297, 298, 1676
BcoDI GTCTC 6 cut(s) 238, 249, 2346, 2546, 2678, 3356
BcuI ACTAGT 1 cut(s) 3952
BfaI CTAG 5 cut(s) 60, 1101, 1365, 3200, 3953
BfmI CTRYAG 4 cut(s) 611, 912, 1501, 2010
BfoI RGCGCY 2 cut(s) 513, 3073
BfuAI ACCTGC 1 cut(s) 950
BglI GCCNNNNNGGC 1 cut(s) 3491
BlpI GCTNAGC 1 cut(s) 1175
Bme18I GGWCC 5 cut(s) 553, 2418, 2873, 2948, 4040
BmeRI GACNNNNNGTC 1 cut(s) 2559
BmeT110I CYCGRG 2 cut(s) 296, 3794
BmgBI CACGTC 2 cut(s) 259, 577
BmsI GCATC 7 cut(s) 229, 798, 1302, 1784, 2908, 3763, 4026
BmtI GCTAGC 1 cut(s) 3203
BpiI GAAGAC 5 cut(s) 579, 1148, 2651, 3317, 3597
BplI GAGNNNNNCTC 4 cut(s) 475, 507, 3435, 3467
BpmI CTGGAG 9 cut(s) 719, 737, 1379, 1649, 2307, 2388, 2901, 3917, 4304
Bpu10I CCTNAGC 3 cut(s) 364, 676, 2832
Bpu1102I GCTNAGC 1 cut(s) 1175
Bpu14I TTCGAA 2 cut(s) 761, 1090
BpuMI CCSGG 3 cut(s) 297, 298, 1676
BsaAI YACGTR 6 cut(s) 222, 2711, 3152, 3468, 3650, 4067
BsaBI GATNNNNATC 2 cut(s) 2295, 2586
BsaHI GRCGYC 5 cut(s) 201, 510, 591, 3070, 3960
BsaI GGTCTC 2 cut(s) 2346, 2678
BsaWI WCCGGW 3 cut(s) 251, 555, 568
BsaXI ACNNNNNCTCC 6 cut(s) 675, 705, 3742, 3753, 3772, 3783
Bse118I RCCGGY 4 cut(s) 139, 512, 2240, 4055
Bse1I ACTGG 6 cut(s) 355, 820, 1362, 1765, 1798, 2290
Bse3DI GCAATG 5 cut(s) 946, 1642, 1831, 3018, 4318
Bse8I GATNNNNATC 2 cut(s) 2295, 2586
BseAI TCCGGA 1 cut(s) 568
BseJI GATNNNNATC 2 cut(s) 2295, 2586
BseMI GCAATG 5 cut(s) 946, 1642, 1831, 3018, 4318
BseMII CTCAG 6 cut(s) 818, 1396, 2771, 2823, 2961, 3488
BseNI ACTGG 6 cut(s) 355, 820, 1362, 1765, 1798, 2290
BseRI GAGGAG 6 cut(s) 56, 1636, 2666, 2849, 2904, 3243
BseSI GKGCMC 2 cut(s) 2479, 2856
BseXI GCAGC 4 cut(s) 597, 798, 1775, 1855
BseYI CCCAGC 1 cut(s) 2746
BsgI GTGCAG 3 cut(s) 166, 1774, 2538
Bsh1236I CGCG 2 cut(s) 534, 539
BshFI GGCC 8 cut(s) 531, 1268, 1519, 1539, 1701, 2644, 2745, 4059
BshNI GGYRCC 4 cut(s) 509, 2237, 3069, 3384
BsiHKAI GWGCWC 3 cut(s) 405, 2856, 3157
BsiHKCI CYCGRG 2 cut(s) 296, 3794
BslFI GGGAC 5 cut(s) 502, 1097, 2392, 2431, 2824
BsmAI GTCTC 6 cut(s) 238, 249, 2346, 2546, 2678, 3356
BsmBI CGTCTC 1 cut(s) 249
BsmFI GGGAC 5 cut(s) 502, 1097, 2392, 2431, 2824
BsmI GAATGC 2 cut(s) 1924, 3412
BsnI GGCC 8 cut(s) 531, 1268, 1519, 1539, 1701, 2644, 2745, 4059
Bso31I GGTCTC 2 cut(s) 2346, 2678
BsoBI CYCGRG 2 cut(s) 296, 3794
Bsp119I TTCGAA 2 cut(s) 761, 1090
Bsp1286I GDGCHC 4 cut(s) 405, 2479, 2856, 3157
Bsp13I TCCGGA 1 cut(s) 568
Bsp1407I TGTACA 3 cut(s) 3015, 3511, 4305
Bsp1720I GCTNAGC 1 cut(s) 1175
BspANI GGCC 8 cut(s) 531, 1268, 1519, 1539, 1701, 2644, 2745, 4059
BspCNI CTCAG 6 cut(s) 817, 1395, 2770, 2824, 2962, 3489
BspEI TCCGGA 1 cut(s) 568
BspFNI CGCG 2 cut(s) 534, 539
BspHI TCATGA 1 cut(s) 3354
BspMAI CTGCAG 2 cut(s) 615, 916
BspMI ACCTGC 1 cut(s) 950
BspOI GCTAGC 1 cut(s) 3203
BspPI GGATC 5 cut(s) 1531, 1707, 1720, 3361, 3873
BspT104I TTCGAA 2 cut(s) 761, 1090
BspT107I GGYRCC 4 cut(s) 509, 2237, 3069, 3384
BspTNI GGTCTC 2 cut(s) 2346, 2678
BsrBI CCGCTC 3 cut(s) 163, 486, 4299
BsrDI GCAATG 5 cut(s) 946, 1642, 1831, 3018, 4318
BsrFI RCCGGY 4 cut(s) 139, 512, 2240, 4055
BsrGI TGTACA 3 cut(s) 3015, 3511, 4305
BsrI ACTGG 6 cut(s) 355, 820, 1362, 1765, 1798, 2290
BssAI RCCGGY 4 cut(s) 139, 512, 2240, 4055
BssNAI GTATAC 1 cut(s) 4070
BssNI GRCGYC 5 cut(s) 201, 510, 591, 3070, 3960
BssSI CACGAG 2 cut(s) 404, 3582
BssT1I CCWWGG 4 cut(s) 1542, 2645, 2894, 3220
Bst1107I GTATAC 1 cut(s) 4070
Bst2BI CACGAG 2 cut(s) 404, 3582
Bst6I CTCTTC 5 cut(s) 122, 868, 2165, 2613, 3636
BstACI GRCGYC 5 cut(s) 201, 510, 591, 3070, 3960
BstAPI GCANNNNNTGC 3 cut(s) 945, 965, 3094
BstAUI TGTACA 3 cut(s) 3015, 3511, 4305
BstBAI YACGTR 6 cut(s) 222, 2711, 3152, 3468, 3650, 4067
BstBI TTCGAA 2 cut(s) 761, 1090
BstDSI CCRYGG 2 cut(s) 532, 3430
BstENI CCTNNNNNAGG 1 cut(s) 3291
BstFNI CGCG 2 cut(s) 534, 539
BstH2I RGCGCY 2 cut(s) 513, 3073
BstHHI GCGC 5 cut(s) 512, 539, 1275, 3072, 3412
BstMAI GTCTC 6 cut(s) 238, 249, 2346, 2546, 2678, 3356
BstNSI RCATGY 7 cut(s) 1279, 1875, 2400, 3518, 3759, 4076, 4274
BstSFI CTRYAG 4 cut(s) 611, 912, 1501, 2010
BstSLI GKGCMC 2 cut(s) 2479, 2856
BstUI CGCG 2 cut(s) 534, 539
BstV1I GCAGC 4 cut(s) 597, 798, 1775, 1855
BstV2I GAAGAC 5 cut(s) 579, 1148, 2651, 3317, 3597
BstX2I RGATCY 3 cut(s) 1523, 1712, 3865
BstXI CCANNNNNNTGG 1 cut(s) 1760
BstYI RGATCY 3 cut(s) 1523, 1712, 3865
BstZ17I GTATAC 1 cut(s) 4070
BsuRI GGCC 8 cut(s) 531, 1268, 1519, 1539, 1701, 2644, 2745, 4059
BtgI CCRYGG 2 cut(s) 532, 3430
BtrI CACGTC 2 cut(s) 259, 577
BtsI GCAGTG 1 cut(s) 1843
BveI ACCTGC 1 cut(s) 950
CciI TCATGA 1 cut(s) 3354
CfoI GCGC 5 cut(s) 512, 539, 1275, 3072, 3412
Cfr10I RCCGGY 4 cut(s) 139, 512, 2240, 4055
Cfr42I CCGCGG 1 cut(s) 535
Cfr9I CCCGGG 1 cut(s) 296
CseI GACGC 4 cut(s) 190, 599, 992, 4140
Csp6I GTAC 5 cut(s) 972, 3016, 3385, 3512, 4306
CviQI GTAC 5 cut(s) 972, 3016, 3385, 3512, 4306
DinI GGCGCC 2 cut(s) 511, 3071
DraIII CACNNNGTG 1 cut(s) 222
DrdI GACNNNNNNGTC 1 cut(s) 1152
DriI GACNNNNNGTC 1 cut(s) 2559
DseDI GACNNNNNNGTC 1 cut(s) 1152
Eam1104I CTCTTC 5 cut(s) 122, 868, 2165, 2613, 3636
Eam1105I GACNNNNNGTC 1 cut(s) 2559
EarI CTCTTC 5 cut(s) 122, 868, 2165, 2613, 3636
EciI GGCGGA 2 cut(s) 135, 783
Ecl136II GAGCTC 1 cut(s) 403
Eco130I CCWWGG 4 cut(s) 1542, 2645, 2894, 3220
Eco24I GRGCYC 1 cut(s) 405
Eco31I GGTCTC 2 cut(s) 2346, 2678
Eco32I GATATC 2 cut(s) 1687, 2588
Eco47I GGWCC 5 cut(s) 553, 2418, 2873, 2948, 4040
Eco53kI GAGCTC 1 cut(s) 403
Eco57I CTGAAG 2 cut(s) 1245, 1647
Eco72I CACGTG 3 cut(s) 222, 3152, 3650
Eco88I CYCGRG 2 cut(s) 296, 3794
EcoICRI GAGCTC 1 cut(s) 403
EcoNI CCTNNNNNAGG 1 cut(s) 3291
EcoO109I RGGNCCY 2 cut(s) 1266, 2418
EcoRI GAATTC 2 cut(s) 1303, 2256
EcoRV GATATC 2 cut(s) 1687, 2588
EcoT14I CCWWGG 4 cut(s) 1542, 2645, 2894, 3220
EcoT22I ATGCAT 1 cut(s) 2576
EcoT38I GRGCYC 1 cut(s) 405
EgeI GGCGCC 2 cut(s) 511, 3071
EheI GGCGCC 2 cut(s) 511, 3071
ErhI CCWWGG 4 cut(s) 1542, 2645, 2894, 3220
Esp3I CGTCTC 1 cut(s) 249
FaqI GGGAC 5 cut(s) 502, 1097, 2392, 2431, 2824
FauI CCCGC 5 cut(s) 174, 454, 479, 509, 4216
FauNDI CATATG 1 cut(s) 1912
FbaI TGATCA 2 cut(s) 2500, 3121
FblI GTMKAC 1 cut(s) 4069
FriOI GRGCYC 1 cut(s) 405
FspBI CTAG 5 cut(s) 60, 1101, 1365, 3200, 3953
FspI TGCGCA 2 cut(s) 1274, 3411
GlaI GCGC 5 cut(s) 511, 538, 1274, 3071, 3411
GsaI CCCAGC 1 cut(s) 2750
GsuI CTGGAG 9 cut(s) 719, 737, 1379, 1649, 2307, 2388, 2901, 3917, 4304
HaeII RGCGCY 2 cut(s) 513, 3073
HaeIII GGCC 8 cut(s) 531, 1268, 1519, 1539, 1701, 2644, 2745, 4059
HgaI GACGC 4 cut(s) 190, 599, 992, 4140
HhaI GCGC 5 cut(s) 512, 539, 1275, 3072, 3412
Hin1I GRCGYC 5 cut(s) 201, 510, 591, 3070, 3960
Hin6I GCGC 5 cut(s) 510, 537, 1273, 3070, 3410
HinP1I GCGC 5 cut(s) 510, 537, 1273, 3070, 3410
HincII GTYRAC 2 cut(s) 343, 1026
HindII GTYRAC 2 cut(s) 343, 1026
HindIII AAGCTT 4 cut(s) 421, 563, 1177, 2096
HpaI GTTAAC 1 cut(s) 343
HphI GGTGA 9 cut(s) 241, 313, 454, 733, 761, 2062, 2075, 2195, 3308
Hpy99I CGWCG 9 cut(s) 86, 175, 191, 206, 379, 382, 593, 610, 652
HpyAV CCTTC 7 cut(s) 1892, 2036, 2312, 2939, 3158, 3429, 3741
Hsp92I GRCGYC 5 cut(s) 201, 510, 591, 3070, 3960
HspAI GCGC 5 cut(s) 510, 537, 1273, 3070, 3410
KasI GGCGCC 2 cut(s) 509, 3069
Kpn2I TCCGGA 1 cut(s) 568
KpnI GGTACC 1 cut(s) 3388
KroI GCCGGC 3 cut(s) 139, 512, 4055
KroNI GCCGGC 3 cut(s) 141, 514, 4057
Ksp22I TGATCA 2 cut(s) 2500, 3121
KspAI GTTAAC 1 cut(s) 343
KspI CCGCGG 1 cut(s) 535
Lsp1109I GCAGC 4 cut(s) 597, 798, 1775, 1855
LweI GCATC 7 cut(s) 229, 798, 1302, 1784, 2908, 3763, 4026
MaeI CTAG 5 cut(s) 60, 1101, 1365, 3200, 3953
MbiI CCGCTC 3 cut(s) 163, 486, 4299
MfeI CAATTG 4 cut(s) 882, 2304, 3630, 4264
MflI RGATCY 3 cut(s) 1523, 1712, 3865
MhlI GDGCHC 4 cut(s) 405, 2479, 2856, 3157
Mly113I GGCGCC 2 cut(s) 510, 3070
MlyI GAGTC 8 cut(s) 313, 484, 1135, 1140, 1397, 2846, 3367, 3653
MmeI TCCRAC 1 cut(s) 3835
Mph1103I ATGCAT 1 cut(s) 2576
MreI CGCCGGCG 1 cut(s) 512
MroI TCCGGA 1 cut(s) 568
MroNI GCCGGC 3 cut(s) 139, 512, 4055
MroXI GAANNNNTTC 2 cut(s) 1041, 3105
MseI TTAA 9 cut(s) 342, 798, 2117, 2174, 3171, 3194, 4097, 4101, 4140
MslI CAYNNNNRTG 3 cut(s) 1407, 2847, 3887
MspA1I CMGCKG 5 cut(s) 534, 1970, 2441, 3188, 3937
MunI CAATTG 4 cut(s) 882, 2304, 3630, 4264
Mva1269I GAATGC 2 cut(s) 1924, 3412
MvnI CGCG 2 cut(s) 534, 539
NaeI GCCGGC 3 cut(s) 141, 514, 4057
NarI GGCGCC 2 cut(s) 510, 3070
NciI CCSGG 3 cut(s) 297, 298, 1676
NdeI CATATG 1 cut(s) 1912
NgoMIV GCCGGC 3 cut(s) 139, 512, 4055
NheI GCTAGC 1 cut(s) 3199
NmeAIII GCCGAG 4 cut(s) 174, 416, 662, 2575
NmuCI GTSAC 6 cut(s) 1163, 1491, 2050, 2383, 3343, 3645
NsbI TGCGCA 2 cut(s) 1274, 3411
NsiI ATGCAT 1 cut(s) 2576
NspI RCATGY 7 cut(s) 1279, 1875, 2400, 3518, 3759, 4076, 4274
NspV TTCGAA 2 cut(s) 761, 1090
PacI TTAATTAA 1 cut(s) 4101
PaeI GCATGC 1 cut(s) 2400
PaeR7I CTCGAG 1 cut(s) 3794
PagI TCATGA 1 cut(s) 3354
PaqCI CACCTGC 1 cut(s) 950
PciI ACATGT 1 cut(s) 3514
PcsI WCGNNNNNNNCGW 3 cut(s) 90, 3142, 3742
PctI GAATGC 2 cut(s) 1924, 3412
PdiI GCCGGC 3 cut(s) 141, 514, 4057
PdmI GAANNNNTTC 2 cut(s) 1041, 3105
PflMI CCANNNNNTGG 1 cut(s) 3057
PleI GAGTC 8 cut(s) 313, 484, 1134, 1139, 1396, 2845, 3366, 3652
PluTI GGCGCC 2 cut(s) 513, 3073
PmaCI CACGTG 3 cut(s) 222, 3152, 3650
PmlI CACGTG 3 cut(s) 222, 3152, 3650
PpsI GAGTC 8 cut(s) 313, 484, 1134, 1139, 1396, 2845, 3366, 3652
Ppu21I YACGTR 6 cut(s) 222, 2711, 3152, 3468, 3650, 4067
PpuMI RGGWCCY 1 cut(s) 2418
PscI ACATGT 1 cut(s) 3514
PshBI ATTAAT 1 cut(s) 4101
PsiI TTATAA 1 cut(s) 2451
Psp124BI GAGCTC 1 cut(s) 405
Psp1406I AACGTT 2 cut(s) 1815, 3597
Psp5II RGGWCCY 1 cut(s) 2418
PspCI CACGTG 3 cut(s) 222, 3152, 3650
PspFI CCCAGC 1 cut(s) 2746
PspPPI RGGWCCY 1 cut(s) 2418
PspXI VCTCGAGB 1 cut(s) 3794
PstI CTGCAG 2 cut(s) 615, 916
PsuI RGATCY 3 cut(s) 1523, 1712, 3865
PvuII CAGCTG 3 cut(s) 1970, 2441, 3937
RsaI GTAC 5 cut(s) 973, 3017, 3386, 3513, 4307
RsaNI GTAC 5 cut(s) 972, 3016, 3385, 3512, 4306
RseI CAYNNNNRTG 3 cut(s) 1407, 2847, 3887
SacI GAGCTC 1 cut(s) 405
SacII CCGCGG 1 cut(s) 535
SaqAI TTAA 9 cut(s) 342, 798, 2117, 2174, 3171, 3194, 4097, 4101, 4140
SchI GAGTC 8 cut(s) 313, 484, 1135, 1140, 1397, 2846, 3367, 3653
SduI GDGCHC 4 cut(s) 405, 2479, 2856, 3157
SfaNI GCATC 7 cut(s) 229, 798, 1302, 1784, 2908, 3763, 4026
SfcI CTRYAG 4 cut(s) 611, 912, 1501, 2010
SfoI GGCGCC 2 cut(s) 511, 3071
Sfr274I CTCGAG 1 cut(s) 3794
Sfr303I CCGCGG 1 cut(s) 535
SfuI TTCGAA 2 cut(s) 761, 1090
SgrAI CRCCGGYG 1 cut(s) 512
SgrBI CCGCGG 1 cut(s) 535
SinI GGWCC 5 cut(s) 553, 2418, 2873, 2948, 4040
SlaI CTCGAG 1 cut(s) 3794
SmaI CCCGGG 1 cut(s) 298
SmiMI CAYNNNNRTG 3 cut(s) 1407, 2847, 3887
SmlI CTYRAG 1 cut(s) 3794
SmoI CTYRAG 1 cut(s) 3794
SpeI ACTAGT 1 cut(s) 3952
SphI GCATGC 1 cut(s) 2400
SspDI GGCGCC 2 cut(s) 509, 3069
SspI AATATT 1 cut(s) 3340
SspMI CTAG 5 cut(s) 60, 1101, 1365, 3200, 3953
SstI GAGCTC 1 cut(s) 405
StyI CCWWGG 4 cut(s) 1542, 2645, 2894, 3220
TaqI TCGA 9 cut(s) 380, 647, 761, 994, 1090, 1129, 3109, 3365, 3795
TatI WGTACW 3 cut(s) 3015, 3511, 4305
Tru1I TTAA 9 cut(s) 342, 798, 2117, 2174, 3171, 3194, 4097, 4101, 4140
Tru9I TTAA 9 cut(s) 342, 798, 2117, 2174, 3171, 3194, 4097, 4101, 4140
TseFI GTSAC 6 cut(s) 1163, 1491, 2050, 2383, 3343, 3645
TseI GCWGC 4 cut(s) 610, 786, 1788, 1868
Tsp45I GTSAC 6 cut(s) 1163, 1491, 2050, 2383, 3343, 3645
TspGWI ACGGA 1 cut(s) 1705
TspMI CCCGGG 1 cut(s) 296
Van91I CCANNNNNTGG 1 cut(s) 3057
VneI GTGCAC 1 cut(s) 2852
VpaK11BI GGWCC 5 cut(s) 553, 2418, 2873, 2948, 4040
VspI ATTAAT 1 cut(s) 4101
XagI CCTNNNNNAGG 1 cut(s) 3291
XapI RAATTY 7 cut(s) 1303, 1414, 1905, 2256, 2717, 3101, 4242
XceI RCATGY 7 cut(s) 1279, 1875, 2400, 3518, 3759, 4076, 4274
XcmI CCANNNNNNNNNTGG 4 cut(s) 2363, 3378, 3869, 4176
XhoI CTCGAG 1 cut(s) 3794
XmaI CCCGGG 1 cut(s) 296
XmiI GTMKAC 1 cut(s) 4069
XmnI GAANNNNTTC 2 cut(s) 1041, 3105
XspI CTAG 5 cut(s) 60, 1101, 1365, 3200, 3953
ZraI GACGTC 1 cut(s) 3961
Zsp2I ATGCAT 1 cut(s) 2576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.