Rh2BG070700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002500
Physical Location & Seq
Reverse (-)
5316402 .. 5326582
10181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG070700.1

Sequence Viewer

Length: 4323 bp
ATGGTTCCTTTTGCCTCAGTTCTCCACCACCTTTTCATTCTCAGCCTCTTCACCCTCTTCACCATAAACCCTACTCTCTCCACTGATTTGGGCAATGAATTCTCAATCCTTAGCTTCGATTCCGATGACTTTCGTGGAGACTACGGGCCTCCATCGCCACCGCCGAGTCCGCCGGAGCCTCAGCCGCCCTCACTCTCCTGCAGAGAAGGTCTCAATGCAACTGGGTCTTTCGACACCATTTGTGAGCTCAATTCAAGCTTGAGTCTTGAAAATGATGTGTATATACATGGAAATGGGAGCTTTTATATCCTCCCTGGTGTTAATGTGAGCTGCCCACATTTGGGGTGCTCCATTGTGGTTAATATTAGTGGAGATTTTAGTTTAGGTAGCAATTCAGTGATTATTGCCGGGTTAGTTTCGGTTAATGCTTCCAATGTTAGCTTGTTTACTGGTTCAGTGATTAATGTGACCGGGATGGCGGGGGCGCCGCCTCCAATGACTAGTGGCACACCAGGGGGTGAGCAGGGTGCTGGTGGAGGTCATGGTGGGAGAGGAGCTAGTTGTGTCACAGATAACAAGAAGCTCCCGGATGATATTTGGGGTGGGGATCCATATGCTTGGTCATCGTTGGAGGAGCCGAAAAGTTATGGGAGTAAGGGTGGGAGTACTAGTAAGGAAGTGAAGTATGGTGGGGAAGGAGGTGGAAGGATTTGGTTGGAGACGAGAAACTCGATTGTTGTTGGGGGGAGTGTTTTGGCTGATGGTGGTGATGGTGGTATCAAGGGTGGAGGAGGGTCAGGAGGCAGCATATACATCAAAGGTCTTAGAATGACTGGAAGTGGCATGATAAGTGCAATTGGGGGTAATGGATTTGCTGGAGGTGGAGGTGGAAGAATTTCTATCAATGTTTTCAGCAGGCGTGATGAACCAGAAATCTTCAGTCATGGGGGAAGGAGTTTAGGCTGTCCTGAAAATGCAGGTGCTGCAGGGACATATTATGATGCTGTCCCTCGAAGGCTTATAGTCAGTAATCATAACTTGTCCACACGGACTGACACGCTTCTGTTGGAGTTTCCTAAACTGCCCCTCTGGACAAATGTTGATATACAAGATCATGCCAAGGCTCTGGTTCCTCTGTTTTGGAGCCGTGTTCAGGTTAGAGGTCAAATTCGTTTAACATGTGGTGCAGTTTTGAGTTTTGGTCTTCAACATTTTGCTTCATCGGAGTTTGAGTTAATGGCAGAAGAGCTTGTAATGAGTGATTCTATCGTCAAGATATTTGGGGCTCTTCGAATGTCTGTGAAAATGCACTTGATGTGGAATTCCAAAATGCTTATAGAGGGTGGTGTTGATGCACTTGTGGCAACATCCTTGCTTGAAGCAACCAATTTAATGGTTCTCAGGGAGTCCTCTGTGATACATTCTACTGCGAATTTGGGAGTTCATGGACAAGGTTTCTTGAATTTGACTGGGCCAGGAGATGTTATTGAAGCAAAACACCTGGTTCTGTCTCTATTTTTTAGTATCAATGTTGGACCTGGATCTTTTCTGCGAGGGCCTTTGGATCGCAGCAGTAGTAACTTTACGACACCCCAACTCAACTGTGAGCTCCCAAATTGCCCTGTGGAATTACTTCATCCACCTGAAGATTGTAACATGAACTCTACATTGACCTTCACTCTTCAGATTTGTCGAGTTGAATATGTTATTGTTGAAGGCATTATAACTGGATCTGTTATACATTTTCACTGGGTGAGAGATGTAGTTGTACACTCTTCTGGGGTAATTACTGCATCTGGTCTGGGTTGCACTGGTGGGCTGGGTAGAGGAAGATTTTTTGTAAATGGTCTTGGCAGCGGTGGCGGGCATGGAGGCAAAGGTGGAGATGGGTTTTACGACGGAAATTTTATTGAAGGTGGTGTTTCCTATGGAAATGCTGATTTACCTTGTGAACTTGGTAGTGGTAGTGGAAATGATAGTCTAGCTGGTGCAACAGCAGGTGGTGGTATTATTGTAATGGGTTCATTGGAGCGCTCATTGTCTAGTTTCACTCTTGCGGGCTCTCTTAGAGCTGATGGAGAAACCTTTGGAGAAAATTTTGGGGAGAAAGTTGGTCAGACCATTTCAAATATAGGTCCTGGTGGTGGATCCGGTGGAACCATTCTTTTGTTTGTTCAGACATTAGCACTCGGTGATTCTTCTACAATCTCAACTGTTGGTGGGCATGGTAGTCCTAGTGGTGGCGGTGGAGGAGGTGGTGGGAGGATTCACTTCCATTGGTCGGACATACCAGTTGGGGATGCATATCTACCCATAGCAAGCGTGCGAGGAAACATTAATACTGGGGGAGGATTCGGTAGAGGAAATGGGCGTGCTGGACAAAATGGGACTATCACTGGAAGGGCCTGTCCCAGAGGGTTATATGGTATCTTTTGTGAGGAATGCCCTCTTGGCACCTTTAAGAATGTGAGTGGCTCTGCTAGAGCCCTTTGTCATGCTTGTCCTTCTTTTCAGCTTCCGCATCGTGCCATATATGTTACCGTTCGAGGTGGTGTTACTGAGACTCCATGTCCATACAAGTGTGTTTCTGACAGATATCACATGCCAAACTGTTATACAGCAGTTGAAGAATTGGTATACACTTTCGGTGGACCGTGGTTGTTCTGTCTTATTCTGTTAGGTCTCCTCATCCTCTTAGCTCTTGTGCTCAGTGTTGCACGAATGAAATATGTTACTGCAGACGAATTACCGGCTGCTGTACCTGCTCGACGGGGCTCCCGTATAGATCATTCTTTCCCTTTCCTTGAATCATTAAATGAGGTTTTGGAAACAAATAGAAATGAGGAATCTCAGAGTCATGTGCATAGGATGTATTTCATGGGGCCAAATACATTCAGTGAACCTTGGCATCTACCTCATTCCCCTCCAGAACAAATAACAGAGATAGTATATGAGGATGCATTCAATCGGTTTGTGGATGAGATTAATGGTTTAGCTGCTTATCAGTGGTGGGAAGGATCAGTCTACAGCATTCTTTCTATTCTTGCCTATCCACTTGCTTGGTGTTGGCTACAGAGTCGACGGAAACAGAAATTACAACAACTTCGTGAATTTGTTCGATCAGAATATGATCATTCTTGCTTGCGTTCTTGTCGTTCCCGTGCACTATATGAAGGGATTAAGGTGGCTGCAACTACGGATTTGATGCTTGCATATGTGGACTTTTTCCTAGGTGGAGATGAGAAAAGAGCTGGCCTTCCTCCCCGTCTTCATCAGAGATTTCCAATGTCTATAATTTTTGGAGGAGATGGAAGTTACATGGCTCCTTTCTCTCTTCAGAGTGATAACATTCTTACTAGCCTCATGAGTCAGTCTGTTCCACCTACCATCTGGTATCGGCTTGTAGCCGGTCTAAATGCTCAGTTGCGCTTAGTCCGGCATGGACACTTAAAACTGACTTTTAGTCATGTTACTAGCTGGCTTGAAACACATGCAAAACCTACTCTTGGTGCATTTGGCATACATGTTGATCTTGCCTGGTTTCAGCCTACAGCGTCTGGATATTGCCACTTCGGAATCGTGGTTTATGCTATGGAAGTTGAAAGTCTGCCATCTGAATTGGACGGTCAAGGTGCATTCTTTCTACCAGAGCATCAGTCACGCATGCCAAGAAATCATTGGGAAAACCAAATTGAACATTTGAGACTCATCGACCTTTGGATATCTCAAAAGAAGTTTTCTGGAGGGATTTTACACACCAAAGGCTTGAGGACGTTAAAAGAGAAGAAGGCCATATGTTATCCCTTCTCTTTTATACTTTATAATGCTAAACCCATTGGCCATCAGGATCTTGTTGGTTTGGTCATTTCTATTTTACTCTTGGGAGACTTTAGCTTAGTTTTGCTTACTTTGCTCCAGCTGTATTCTATTTCACTCTTAAGTTTATTCTTAGTCTTGTTCATTCTTCCTCTCGGTCTACTATTCCCCTTCCCGGCTGGAATCAGCGCTTTGTTTAGTCATGGGGCTAGACGTTCAGCAGGTCTTGCCCGTTTATATGCTTTGTGGAATCTCACATCCTTGATCAATGTTGTAGTTGCTTTTATATGTGGATTGATTCATTATACGACTCACTCAAATAAAAAGCATTCCAATTTTCAGTCCTGGACTTTTACCATGGATGAAAGTGAATGGTGGGTGCTTCCTTGTGGTCTAGTACTGTGCAAAGTTGTCCAATCACGACTTATTGATTGCCTCGTGGCAAACCAGGAGATTCAGGATCACTCACTGTACAGCAATGACCCTGAACTGTTCTGGCAGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1440

Amino Acids

154.86

Weight (kDa)

6.13

Isoelectric Point (pI)

46.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8003 PF26010 798 - 872 1.5e-34 Domain of unknown function (DUF8003)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1724, 3822
AarI CACCTGC 2 cut(s) 968, 1988
Acc36I ACCTGC 4 cut(s) 968, 1988, 2770, 4028
AccB1I GGYRCC 2 cut(s) 484, 2453
AccI GTMKAC 4 cut(s) 2637, 3024, 3079, 3976
AcoI YGGCCR 1 cut(s) 3838
AcsI RAATTY 9 cut(s) 98, 894, 1167, 1321, 1432, 1462, 1903, 2095, 3110
AcuI CTGAAG 4 cut(s) 922, 1665, 1667, 3320
AcyI GRCGYC 1 cut(s) 485
AdeI CACNNNGTG 2 cut(s) 1753, 2192
AfaI GTAC 5 cut(s) 667, 1770, 2760, 4215, 4289
AfeI AGCGCT 2 cut(s) 2033, 4006
AfiI CCNNNNNNNGG 8 cut(s) 340, 341, 1153, 2144, 2240, 2281, 3506, 3991
AflII CTTAAG 1 cut(s) 3937
AflIII ACRYGT 2 cut(s) 1178, 3523
AhdI GACNNNNNGTC 2 cut(s) 2568, 3462
AhlI ACTAGT 2 cut(s) 500, 668
AjnI CCWGG 9 cut(s) 313, 511, 1474, 1500, 1537, 2137, 3536, 4160, 4263
AjuI GAANNNNNNNTTGG 2 cut(s) 2433, 2465
Alw21I GWGCWC 5 cut(s) 249, 350, 1611, 2709, 3166
Alw26I GTCTC 8 cut(s) 132, 215, 713, 1515, 2555, 2687, 3697, 3879
Alw44I GTGCAC 1 cut(s) 3162
AlwNI CAGNNNCTG 2 cut(s) 983, 3557
Aor51HI AGCGCT 2 cut(s) 2033, 4006
AoxI GGCC 8 cut(s) 146, 1472, 1556, 2403, 2882, 3253, 3789, 3838
ApaLI GTGCAC 1 cut(s) 3162
ApeKI GCWGC 8 cut(s) 330, 804, 983, 1569, 1854, 2753, 2996, 3188
ApoI RAATTY 9 cut(s) 98, 894, 1167, 1321, 1432, 1462, 1903, 2095, 3110
AseI ATTAAT 3 cut(s) 462, 2337, 2985
Asp700I GAANNNNTTC 3 cut(s) 895, 1632, 3114
AspA2I CCTAGG 1 cut(s) 3229
AspLEI GCGC 4 cut(s) 487, 2034, 3429, 4007
AspS9I GGNCC 8 cut(s) 146, 1472, 1535, 1556, 2135, 2403, 2651, 2882
AsuC2I CCSGG 4 cut(s) 409, 472, 587, 3992
AsuHPI GGTGA 6 cut(s) 43, 52, 530, 779, 1765, 2204
AsuII TTCGAA 1 cut(s) 1291
AvaII GGWCC 3 cut(s) 1535, 2135, 2651
AvrII CCTAGG 1 cut(s) 3229
BaeGI GKGCMC 1 cut(s) 3166
BalI TGGCCA 1 cut(s) 3840
BamHI GGATCC 2 cut(s) 607, 2147
BanI GGYRCC 2 cut(s) 484, 2453
BanII GRGCYC 6 cut(s) 249, 1288, 1611, 2063, 2488, 2777
BauI CACGAG 1 cut(s) 4253
BbsI GAAGAC 2 cut(s) 1196, 3260
Bbv12I GWGCWC 5 cut(s) 249, 350, 1611, 2709, 3166
BbvCI CCTCAGC 1 cut(s) 180
BbvI GCAGC 8 cut(s) 317, 816, 970, 1581, 1866, 2740, 2983, 3175
BceAI ACGGC 1 cut(s) 1131
BciT130I CCWGG 9 cut(s) 315, 513, 1476, 1502, 1539, 2139, 3538, 4162, 4265
BclI TGATCA 2 cut(s) 3130, 4080
BcnI CCSGG 4 cut(s) 409, 472, 587, 3992
BcoDI GTCTC 8 cut(s) 132, 215, 713, 1515, 2555, 2687, 3697, 3879
BcuI ACTAGT 2 cut(s) 500, 668
BfmI CTRYAG 6 cut(s) 199, 984, 2736, 3025, 3071, 3549
BfoI RGCGCY 3 cut(s) 488, 2035, 4008
BfrI CTTAAG 1 cut(s) 3937
BfuAI ACCTGC 4 cut(s) 968, 1988, 2770, 4028
BglI GCCNNNNNGGC 1 cut(s) 2451
BlnI CCTAGG 1 cut(s) 3229
BmcAI AGTACT 2 cut(s) 667, 4215
Bme18I GGWCC 3 cut(s) 1535, 2135, 2651
BmeRI GACNNNNNGTC 2 cut(s) 2568, 3462
BmgT120I GGNCC 8 cut(s) 146, 1472, 1535, 1556, 2135, 2403, 2651, 2882
BmrI ACTGGG 4 cut(s) 231, 1479, 1759, 2352
BmsI GCATC 9 cut(s) 991, 1342, 1802, 2290, 2530, 2917, 2947, 3195, 3661
BmuI ACTGGG 4 cut(s) 231, 1479, 1759, 2352
BpiI GAAGAC 2 cut(s) 1196, 3260
BplI GAGNNNNNCTC 2 cut(s) 195, 227
BpmI CTGGAG 4 cut(s) 897, 2910, 3762, 3899
Bpu10I CCTNAGC 2 cut(s) 110, 180
Bpu14I TTCGAA 1 cut(s) 1291
BpuEI CTTGAG 2 cut(s) 280, 3787
BpuMI CCSGG 4 cut(s) 409, 472, 587, 3992
BsaBI GATNNNNATC 1 cut(s) 2304
BsaHI GRCGYC 1 cut(s) 485
BsaI GGTCTC 2 cut(s) 215, 2687
BsaJI CCNNGG 7 cut(s) 313, 512, 1119, 2654, 2903, 3229, 4173
BsaWI WCCGGW 1 cut(s) 2150
BsaXI ACNNNNNCTCC 8 cut(s) 643, 655, 673, 685, 1217, 1247, 2548, 2578
Bsc4I CCNNNNNNNGG 8 cut(s) 340, 341, 1153, 2144, 2240, 2281, 3506, 3991
Bse118I RCCGGY 2 cut(s) 2749, 3407
Bse3DI GCAATG 2 cut(s) 100, 4300
Bse8I GATNNNNATC 1 cut(s) 2304
BseBI CCWGG 9 cut(s) 315, 513, 1476, 1502, 1539, 2139, 3538, 4162, 4265
BseDI CCNNGG 7 cut(s) 313, 512, 1119, 2654, 2903, 3229, 4173
BseJI GATNNNNATC 1 cut(s) 2304
BseLI CCNNNNNNNGG 8 cut(s) 340, 341, 1153, 2144, 2240, 2281, 3506, 3991
BseMI GCAATG 2 cut(s) 100, 4300
BseMII CTCAG 8 cut(s) 30, 55, 194, 1414, 2550, 2722, 2864, 3434
BseRI GAGGAG 6 cut(s) 567, 647, 804, 2265, 2675, 3318
BseSI GKGCMC 1 cut(s) 3166
BseXI GCAGC 8 cut(s) 317, 816, 970, 1581, 1866, 2740, 2983, 3175
BseYI CCCAGC 1 cut(s) 1819
BsgI GTGCAG 1 cut(s) 1206
BshFI GGCC 8 cut(s) 148, 1474, 1558, 2405, 2884, 3255, 3791, 3840
BshNI GGYRCC 2 cut(s) 484, 2453
BsiHKAI GWGCWC 5 cut(s) 249, 350, 1611, 2709, 3166
BsiSI CCGG 9 cut(s) 173, 408, 471, 587, 2151, 2750, 3408, 3436, 3992
BslFI GGGAC 4 cut(s) 992, 1003, 2394, 2401
BslI CCNNNNNNNGG 8 cut(s) 340, 341, 1153, 2144, 2240, 2281, 3506, 3991
BsmAI GTCTC 8 cut(s) 132, 215, 713, 1515, 2555, 2687, 3697, 3879
BsmBI CGTCTC 1 cut(s) 713
BsmFI GGGAC 4 cut(s) 992, 1003, 2394, 2401
BsmI GAATGC 5 cut(s) 2447, 2960, 3030, 3635, 4144
BsnI GGCC 8 cut(s) 148, 1474, 1558, 2405, 2884, 3255, 3791, 3840
Bso31I GGTCTC 2 cut(s) 215, 2687
Bsp119I TTCGAA 1 cut(s) 1291
Bsp1286I GDGCHC 9 cut(s) 249, 350, 1288, 1611, 2063, 2488, 2709, 2777, 3166
Bsp1407I TGTACA 2 cut(s) 1768, 4287
Bsp19I CCATGG 1 cut(s) 4173
BspANI GGCC 8 cut(s) 148, 1474, 1558, 2405, 2884, 3255, 3791, 3840
BspCNI CTCAG 8 cut(s) 29, 54, 193, 1413, 2551, 2721, 2863, 3433
BspHI TCATGA 1 cut(s) 3363
BspMAI CTGCAG 3 cut(s) 203, 988, 2740
BspMI ACCTGC 4 cut(s) 968, 1988, 2770, 4028
BspQI GCTCTTC 2 cut(s) 1239, 1293
BspT104I TTCGAA 1 cut(s) 1291
BspT107I GGYRCC 2 cut(s) 484, 2453
BspTI CTTAAG 1 cut(s) 3937
BspTNI GGTCTC 2 cut(s) 215, 2687
BsrDI GCAATG 2 cut(s) 100, 4300
BsrFI RCCGGY 2 cut(s) 2749, 3407
BsrGI TGTACA 2 cut(s) 1768, 4287
BssAI RCCGGY 2 cut(s) 2749, 3407
BssECI CCNNGG 7 cut(s) 313, 512, 1119, 2654, 2903, 3229, 4173
BssNAI GTATAC 1 cut(s) 2638
BssNI GRCGYC 1 cut(s) 485
BssSI CACGAG 1 cut(s) 4253
BssT1I CCWWGG 4 cut(s) 1119, 2903, 3229, 4173
Bst1107I GTATAC 1 cut(s) 2638
Bst2BI CACGAG 1 cut(s) 4253
Bst2UI CCWGG 9 cut(s) 315, 513, 1476, 1502, 1539, 2139, 3538, 4162, 4265
Bst4CI ACNGT 9 cut(s) 1604, 2215, 2542, 2612, 2655, 3626, 4218, 4287, 4308
Bst6I CTCTTC 7 cut(s) 53, 62, 1239, 1293, 1686, 1780, 3339
BstACI GRCGYC 1 cut(s) 485
BstAFI CTTAAG 1 cut(s) 3937
BstAPI GCANNNNNTGC 2 cut(s) 983, 4043
BstAUI TGTACA 2 cut(s) 1768, 4287
BstBI TTCGAA 1 cut(s) 1291
BstDSI CCRYGG 2 cut(s) 2654, 4173
BstH2I RGCGCY 3 cut(s) 488, 2035, 4008
BstHHI GCGC 4 cut(s) 487, 2034, 3429, 4007
BstMAI GTCTC 8 cut(s) 132, 215, 713, 1515, 2555, 2687, 3697, 3879
BstNI CCWGG 9 cut(s) 315, 513, 1476, 1502, 1539, 2139, 3538, 4162, 4265
BstNSI RCATGY 5 cut(s) 1182, 2605, 3494, 3527, 3667
BstSFI CTRYAG 6 cut(s) 199, 984, 2736, 3025, 3071, 3549
BstSLI GKGCMC 1 cut(s) 3166
BstV1I GCAGC 8 cut(s) 317, 816, 970, 1581, 1866, 2740, 2983, 3175
BstV2I GAAGAC 2 cut(s) 1196, 3260
BstX2I RGATCY 5 cut(s) 607, 1541, 1730, 2147, 3847
BstXI CCANNNNNNTGG 5 cut(s) 88, 618, 1126, 1393, 3060
BstYI RGATCY 5 cut(s) 607, 1541, 1730, 2147, 3847
BstZ17I GTATAC 1 cut(s) 2638
BsuRI GGCC 8 cut(s) 148, 1474, 1558, 2405, 2884, 3255, 3791, 3840
BtgI CCRYGG 2 cut(s) 2654, 4173
BtgZI GCGATG 1 cut(s) 138
BveI ACCTGC 4 cut(s) 968, 1988, 2770, 4028
CaiI CAGNNNCTG 2 cut(s) 983, 3557
CciI TCATGA 1 cut(s) 3363
CfoI GCGC 4 cut(s) 487, 2034, 3429, 4007
Cfr10I RCCGGY 2 cut(s) 2749, 3407
Cfr13I GGNCC 8 cut(s) 146, 1472, 1535, 1556, 2135, 2403, 2651, 2882
CseI GACGC 1 cut(s) 3543
CsiI ACCWGGT 1 cut(s) 1500
Csp6I GTAC 5 cut(s) 666, 1769, 2759, 4214, 4288
CviQI GTAC 5 cut(s) 666, 1769, 2759, 4214, 4288
DinI GGCGCC 1 cut(s) 486
DraIII CACNNNGTG 2 cut(s) 1753, 2192
DriI GACNNNNNGTC 2 cut(s) 2568, 3462
EaeI YGGCCR 1 cut(s) 3838
Eam1104I CTCTTC 7 cut(s) 53, 62, 1239, 1293, 1686, 1780, 3339
Eam1105I GACNNNNNGTC 2 cut(s) 2568, 3462
EarI CTCTTC 7 cut(s) 53, 62, 1239, 1293, 1686, 1780, 3339
EciI GGCGGA 1 cut(s) 159
Ecl136II GAGCTC 2 cut(s) 247, 1609
Eco130I CCWWGG 4 cut(s) 1119, 2903, 3229, 4173
Eco24I GRGCYC 6 cut(s) 249, 1288, 1611, 2063, 2488, 2777
Eco31I GGTCTC 2 cut(s) 215, 2687
Eco32I GATATC 2 cut(s) 2597, 3723
Eco47I GGWCC 3 cut(s) 1535, 2135, 2651
Eco47III AGCGCT 2 cut(s) 2033, 4006
Eco53kI GAGCTC 2 cut(s) 247, 1609
Eco57I CTGAAG 4 cut(s) 922, 1665, 1667, 3320
EcoICRI GAGCTC 2 cut(s) 247, 1609
EcoO109I RGGNCCY 3 cut(s) 1556, 2135, 2403
EcoRI GAATTC 2 cut(s) 98, 1321
EcoRII CCWGG 9 cut(s) 313, 511, 1474, 1500, 1537, 2137, 3536, 4160, 4263
EcoRV GATATC 2 cut(s) 2597, 3723
EcoT14I CCWWGG 4 cut(s) 1119, 2903, 3229, 4173
EcoT22I ATGCAT 2 cut(s) 2305, 2962
EcoT38I GRGCYC 6 cut(s) 249, 1288, 1611, 2063, 2488, 2777
EgeI GGCGCC 1 cut(s) 486
EheI GGCGCC 1 cut(s) 486
ErhI CCWWGG 4 cut(s) 1119, 2903, 3229, 4173
Esp3I CGTCTC 1 cut(s) 713
FaqI GGGAC 4 cut(s) 992, 1003, 2394, 2401
FauI CCCGC 3 cut(s) 472, 1856, 2050
FauNDI CATATG 3 cut(s) 613, 3214, 3794
FbaI TGATCA 2 cut(s) 3130, 4080
FblI GTMKAC 4 cut(s) 2637, 3024, 3079, 3976
FriOI GRGCYC 6 cut(s) 249, 1288, 1611, 2063, 2488, 2777
GlaI GCGC 4 cut(s) 486, 2033, 3428, 4006
GsaI CCCAGC 1 cut(s) 1823
GsuI CTGGAG 4 cut(s) 897, 2910, 3762, 3899
HaeII RGCGCY 3 cut(s) 488, 2035, 4008
HaeIII GGCC 8 cut(s) 148, 1474, 1558, 2405, 2884, 3255, 3791, 3840
HapII CCGG 9 cut(s) 173, 408, 471, 587, 2151, 2750, 3408, 3436, 3992
HgaI GACGC 1 cut(s) 3543
HhaI GCGC 4 cut(s) 487, 2034, 3429, 4007
Hin1I GRCGYC 1 cut(s) 485
Hin6I GCGC 4 cut(s) 485, 2032, 3427, 4005
HinP1I GCGC 4 cut(s) 485, 2032, 3427, 4005
HincII GTYRAC 1 cut(s) 3080
HindII GTYRAC 1 cut(s) 3080
HindIII AAGCTT 1 cut(s) 256
HpaII CCGG 9 cut(s) 173, 408, 471, 587, 2151, 2750, 3408, 3436, 3992
HphI GGTGA 6 cut(s) 43, 52, 530, 779, 1765, 2204
Hpy99I CGWCG 3 cut(s) 1901, 2772, 3084
HpyCH4III ACNGT 9 cut(s) 1604, 2215, 2542, 2612, 2655, 3626, 4218, 4287, 4308
HpyCH4IV ACGT 2 cut(s) 3773, 4030
HpySE526I ACGT 2 cut(s) 3773, 4030
Hsp92I GRCGYC 1 cut(s) 485
HspAI GCGC 4 cut(s) 485, 2032, 3427, 4005
KasI GGCGCC 1 cut(s) 484
Ksp22I TGATCA 2 cut(s) 3130, 4080
LguI GCTCTTC 2 cut(s) 1239, 1293
Lsp1109I GCAGC 8 cut(s) 317, 816, 970, 1581, 1866, 2740, 2983, 3175
LweI GCATC 9 cut(s) 991, 1342, 1802, 2290, 2530, 2917, 2947, 3195, 3661
MabI ACCWGGT 1 cut(s) 1500
MaeII ACGT 2 cut(s) 3773, 4030
MfeI CAATTG 1 cut(s) 855
MflI RGATCY 5 cut(s) 607, 1541, 1730, 2147, 3847
MhlI GDGCHC 9 cut(s) 249, 350, 1288, 1611, 2063, 2488, 2709, 2777, 3166
MlsI TGGCCA 1 cut(s) 3840
MluNI TGGCCA 1 cut(s) 3840
Mly113I GGCGCC 1 cut(s) 485
MlyI GAGTC 9 cut(s) 175, 271, 1415, 2557, 2863, 3085, 3376, 3699, 4120
MmeI TCCRAC 5 cut(s) 609, 696, 1047, 1513, 2262
Mox20I TGGCCA 1 cut(s) 3840
Mph1103I ATGCAT 2 cut(s) 2305, 2962
MroXI GAANNNNTTC 3 cut(s) 895, 1632, 3114
MscI TGGCCA 1 cut(s) 3840
MslI CAYNNNNRTG 2 cut(s) 291, 2578
Msp20I TGGCCA 1 cut(s) 3840
MspA1I CMGCKG 2 cut(s) 1857, 3919
MspCI CTTAAG 1 cut(s) 3937
MspI CCGG 9 cut(s) 173, 408, 471, 587, 2151, 2750, 3408, 3436, 3992
MunI CAATTG 1 cut(s) 855
Mva1269I GAATGC 5 cut(s) 2447, 2960, 3030, 3635, 4144
MvaI CCWGG 9 cut(s) 315, 513, 1476, 1502, 1539, 2139, 3538, 4162, 4265
NarI GGCGCC 1 cut(s) 485
NciI CCSGG 4 cut(s) 409, 472, 587, 3992
NcoI CCATGG 1 cut(s) 4173
NdeI CATATG 3 cut(s) 613, 3214, 3794
NmeAIII GCCGAG 1 cut(s) 189
NmuCI GTSAC 3 cut(s) 466, 565, 3657
NsiI ATGCAT 2 cut(s) 2305, 2962
NspI RCATGY 5 cut(s) 1182, 2605, 3494, 3527, 3667
NspV TTCGAA 1 cut(s) 1291
PaeI GCATGC 1 cut(s) 3667
PagI TCATGA 1 cut(s) 3363
PaqCI CACCTGC 2 cut(s) 968, 1988
PciI ACATGT 2 cut(s) 1178, 3523
PciSI GCTCTTC 2 cut(s) 1239, 1293
PcsI WCGNNNNNNNCGW 3 cut(s) 161, 728, 2776
PctI GAATGC 5 cut(s) 2447, 2960, 3030, 3635, 4144
PdmI GAANNNNTTC 3 cut(s) 895, 1632, 3114
PfoI TCCNGGA 2 cut(s) 585, 4160
PleI GAGTC 9 cut(s) 174, 270, 1414, 2557, 2862, 3084, 3375, 3699, 4120
PluTI GGCGCC 1 cut(s) 488
PpsI GAGTC 9 cut(s) 174, 270, 1414, 2557, 2862, 3084, 3375, 3699, 4120
PpuMI RGGWCCY 1 cut(s) 2135
PscI ACATGT 2 cut(s) 1178, 3523
PshBI ATTAAT 3 cut(s) 462, 2337, 2985
PsiI TTATAA 2 cut(s) 1724, 3822
Psp124BI GAGCTC 2 cut(s) 249, 1611
Psp5II RGGWCCY 1 cut(s) 2135
Psp6I CCWGG 9 cut(s) 313, 511, 1474, 1500, 1537, 2137, 3536, 4160, 4263
PspFI CCCAGC 1 cut(s) 1819
PspGI CCWGG 9 cut(s) 313, 511, 1474, 1500, 1537, 2137, 3536, 4160, 4263
PspPI GGNCC 8 cut(s) 146, 1472, 1535, 1556, 2135, 2403, 2651, 2882
PspPPI RGGWCCY 1 cut(s) 2135
PstI CTGCAG 3 cut(s) 203, 988, 2740
PstNI CAGNNNCTG 2 cut(s) 983, 3557
PsuI RGATCY 5 cut(s) 607, 1541, 1730, 2147, 3847
PvuII CAGCTG 1 cut(s) 3919
RsaI GTAC 5 cut(s) 667, 1770, 2760, 4215, 4289
RsaNI GTAC 5 cut(s) 666, 1769, 2759, 4214, 4288
RseI CAYNNNNRTG 2 cut(s) 291, 2578
SacI GAGCTC 2 cut(s) 249, 1611
SalI GTCGAC 1 cut(s) 3078
SapI GCTCTTC 2 cut(s) 1239, 1293
Sau96I GGNCC 8 cut(s) 146, 1472, 1535, 1556, 2135, 2403, 2651, 2882
ScaI AGTACT 2 cut(s) 667, 4215
SchI GAGTC 9 cut(s) 175, 271, 1415, 2557, 2863, 3085, 3376, 3699, 4120
SduI GDGCHC 9 cut(s) 249, 350, 1288, 1611, 2063, 2488, 2709, 2777, 3166
SexAI ACCWGGT 1 cut(s) 1500
SfaNI GCATC 9 cut(s) 991, 1342, 1802, 2290, 2530, 2917, 2947, 3195, 3661
SfcI CTRYAG 6 cut(s) 199, 984, 2736, 3025, 3071, 3549
SfoI GGCGCC 1 cut(s) 486
SfuI TTCGAA 1 cut(s) 1291
SinI GGWCC 3 cut(s) 1535, 2135, 2651
SmiMI CAYNNNNRTG 2 cut(s) 291, 2578
SmlI CTYRAG 3 cut(s) 259, 3766, 3937
SmoI CTYRAG 3 cut(s) 259, 3766, 3937
SpeI ACTAGT 2 cut(s) 500, 668
SphI GCATGC 1 cut(s) 3667
SspDI GGCGCC 1 cut(s) 484
SspI AATATT 1 cut(s) 364
SstI GAGCTC 2 cut(s) 249, 1611
StyI CCWWGG 4 cut(s) 1119, 2903, 3229, 4173
TaaI ACNGT 9 cut(s) 1604, 2215, 2542, 2612, 2655, 3626, 4218, 4287, 4308
TaiI ACGT 2 cut(s) 3776, 4033
TaqII GACCGA 1 cut(s) 3962
TatI WGTACW 4 cut(s) 665, 1768, 4213, 4287
TauI GCSGC 2 cut(s) 187, 490
TseFI GTSAC 3 cut(s) 466, 565, 3657
TseI GCWGC 8 cut(s) 330, 804, 983, 1569, 1854, 2753, 2996, 3188
Tsp45I GTSAC 3 cut(s) 466, 565, 3657
TspGWI ACGGA 4 cut(s) 1063, 1914, 3097, 3212
Vha464I CTTAAG 1 cut(s) 3937
VneI GTGCAC 1 cut(s) 3162
VpaK11BI GGWCC 3 cut(s) 1535, 2135, 2651
VspI ATTAAT 3 cut(s) 462, 2337, 2985
XapI RAATTY 9 cut(s) 98, 894, 1167, 1321, 1432, 1462, 1903, 2095, 3110
XceI RCATGY 5 cut(s) 1182, 2605, 3494, 3527, 3667
XcmI CCANNNNNNNNNTGG 2 cut(s) 3387, 3675
XmaJI CCTAGG 1 cut(s) 3229
XmiI GTMKAC 4 cut(s) 2637, 3024, 3079, 3976
XmnI GAANNNNTTC 3 cut(s) 895, 1632, 3114
ZrmI AGTACT 2 cut(s) 667, 4215
Zsp2I ATGCAT 2 cut(s) 2305, 2962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.