Rw6G040550

glycine-rich protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002500
Physical Location & Seq
Reverse (-)
64301480 .. 64310800
9321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G040550.1

Sequence Viewer

Length: 4272 bp
ATGGCTCGCTTTCACCCTCCCACTCTTCATTTCGCATTCTGCTTCATCCTTATAGCTCTAACTACAAACCCTAGCCTCCTCCTTGCATCCGAAGACGACGACGAGTTTTCTGATTCCAATCTCTTCCACCAGGATTACTCGCCACCGGCTCCGCCGCCGCCGCCGCCGCACCCGCCGTCGGTGTCCTGCACCGATGACCTCGGCGGCGTCGGCTCGCTGGACACCACGTGTCAGATAGTCTCTGATTTGAACCTCACCAGAGACGTGTATATAGCAGGGAAAGGAAATTTTTATATCCTACCCGGTGTGAGATTCTATTGCGATACTCCTGGTTGCTCCATAATCGTTAACGTTTCTGGTAATTTTAGCTTAGGTAACTCGTCGTCGGTTTTCGCCGGAGCTTTTGAGGTCGTGGCGCACAATGCCAGCTTTCTCAACGGCTCAGTGGTGAACACGACGGCTCTGGCGGGGATACCGCCGCCCCAGACGAGCGGGACCCCGCAGGGGATAGACGGCGCGGGCGGCGGCCATGGAGGGCGCGGCGCGTGTTGTTTGGTGGACCATTCGAAGCTTCCGGAAGACGTGTGGGGCGGCGACGCCTACTCGTGGTCGTCGCTGCAGAGGCCGTGTAGTTTTGGGAGCAGAGGCGGTTCGACGAGTAAGGAAGTGGATTATGGAGGCTTAGGGGGAGGGATAGTGAAGCTGGTGATTACGAATTTTCTGGAGGTGAATGGGAGTGTTTTGGCTGAGGGAGGTGATGGAGGTGCCAAAGGTGGAGGTGGTTCTGGTGGCAGCATCTACATTAAGGCTCAGAGAATGACTGGTAGTGGCAGGATAAGTGCTTGTGGTGGTAATGGTTATGCTGGTGGCGGTGGTGGAAGAGTGTCAATTGATGTTTTTAGCAGGCATGATGACCCTTCAGTCTTTGTGCATGGAGGAAGTAGCAATGCATGTCCAGAAAATGCAGGCGGTGCTGGTACTTTATATGATGCAGTTCCTCGAAGCCTTATTATTAGCAATCATAACAAGTCAACAGATACAGAAACACTTCTTTTGGAGTTTCCTTATCAGCCTCTTATGACAAATGTATATGTTCGAAATAAAGCTAGGGCTACTGTCCCTCTGCTTTGGAGTCGAGTCCAGGTGCAAGGACAGATAAGTCTTCTCTGTGACAGTGTGCTTAGCTTTGGACTTCAACATTATGCTTCATCAGAATTTGAGTTATTGGCTGAAGAATTGCTAATGAGCGATTCTGTAATCAAGGTGTATGGAGCCTTGCGCATGTCCGTAAAAATGTTCTTGATGTGGAATTCCAAGATGCTTATAGATGGTGGTGGAGAAGAAGCTGTTGACACATCCTTACTTGAGGCTAGTAACTTGGTTGTTCTCAGGGAGTCATCTGTGATACACTCTAATGCAAATTTGGGAGTTCATGGACAAGGGTTACTGAACCTATCAGGACCAGGAGATTGGATTCAAGCACAACGTCTGGTGCTATCACTATTCTACAGTATTCATGTTGGGCCTGGATCTGTTTTGCGTGGCCCCTTAGAGAACGCAACATCTGATTCTGTAACACCAAAGCTCTACTGTGAAAATGAAGATTGCCCGGTTGAGCTACTTCATCCACCTGAAGATTGCAATGTGAACTCCTCATTGTCCTTTACACTTCAGATATGCCGGGTTGAGGATATCAATATTGCAGGCCTGATAAAAGGATCCATTGTTCATTTTCACAGGGCAAGGACCATTGCTGTCCACTCTACTGGAATGATAAGTGCATCAGGAATGGGCTGCACTGGTGGTGTAGGGAGTGGTACCGTCCTAAGCAATGGAATTGGCAGTGGTGGTGGGCATGGTGGTAAAGGTGGGGCTGCATGTATTAACGGTAGTTGTATTGAGGGTGGAATCACATATGGGAATGAAAAGTTGCCTTGTGAACTTGGTAGTGGAAGTGGATATGACAGTTCAGCTGGTTCAACTGCTGGTGGTGGTATTATTGTAATGGGTTCTTTAGAACATCCATTGTCAAGTATGTCTGTTGAAGGCTTGGTGACAGCTGATGGTGAAGATTCTGAAAGTGGACGTCCTGGGGGTGGTTCTGGTGGAACTATACTTCTCTTCTTGCGTACAATAGATCTTGGTAAATCTGCCAATCTCTCGAGTGCTGGGGGATATGGGAGTTCCAATGGGGCCGGTGGTGGTGGTGGTGGAAGAATTCATTTTCATTGGTCAGACATTCCCACTGGAGATGTTTATCAGCCCATTGCAAATGTGGAGGGAAACATTCATGCAGGGGGAGGGACAGGCAGAGACCAGGGTGGTGCTGGAGAACATGGGACAGTGACAGGAAAAGCATGCCCAAAAGGGCTATATGGGACCTTTTGTGAGGAATGTCCAGCTGGCACTTATAAGGATGTCATTGGGTCTGATGGGGCACTTTGTCATGATTGTCCTGCTAAAGACCTTCCTCAACGTGCTATTTATATTTCTGTTCGAGGTGGTGTTGCCGAGACGCCTTGTCCTTACAAATGCATTTCAGACAGATATCACATGCCAAACTGTTATACGGCTCTTGAAGAGTTGATCTACACATTTGGTGGGCCTTGGCTATTTGGTCTTCTTCTGATTGGTCTCCTCATCCTGTTAGCTTTGGTGCTAAGTGTCGCGCGTATGAAATTTGTTGGGGTTGATGAATTACCCGGCCCAGCTCCCACTCAACATGGCTCACAAATAGACCACTCTTTTCCATTTCTTGAATCATTGAACGAGGTTTTGGAAACAAACAGAGCTGAGGAGTCCCATAGCCATGTGCACAGAATGTATTTTATGGGTCCGAATACATTCAGCGAACCTTGGCATCTGCCTCACACTCCTCCAGAACAAATAAAAGAGATTGTATACGAGGGTCCATTCAATACATTTGTTGATGAGATTAATGCCATAGCCAATTATCAGTGGTGGGAGGGAGCAATGTACATCATTCTTGCTGTTCTTGCATATCCCCTTGCATGCTCATGGCAACAGTGGCGCCGAAGATTGAAATTGCAACGCCTGCGTGAATTTGTTCGATCAGAATACGATCATGCCTGCTTACGGTCTTGTCGTTCACGTGCTCTTTATGAAGGAATTAAGGTAGCTGCCACTTCTGATTTAATGCTAGCTTATGTGGACTTCTTCCTTGGTGGGGATGAAAAGAGAACTGATCTTCCTCCTCGTTTACATCAAAGATTTCCTATGTCATTACCTTTTGGGGGTGATGGAAGTTATATGGCTCCTTTCTGTCTTCACAGTGATAATATTGTCACAAGTCTCATGAGTCAGTCGATCCCACCGACCACATGGTACCGTATGGTGGCTGGTTTGAATGCACAATTACGCTTAGTTTGCCGTGGACGGCTAAGAGTTACACTCCTTCCTGTCCTTAGGTGGCTTGAAAGCCATGCCAATCCGGCTTTGAGGAATTATGGTGTACGTGTTGATCTTGCATGGTTTCAGGCTACAGCTTTTGGTTATCGCCATTATGGACTAGTGGTGTATGCTCTTGAAGAAGACCACATTGATGGAGCAATTGGAACTGAAGAATCACGTGCAAATAGCATTTACAATGAAAATTCTTCTGGTCATTTGATAGAAGAACCACTCATAAGCCAGTCTCACAGAAGCAATGTGAGGCGAAGGAGAGCATATGGAGGGATTGTAGATGCCAACAATGTACAAATGCTTATGGAACCGAGAGATATATTTTATCCTGTCTCTCTTATGCTTCATAATACTAAACCCGTTGGACACCAGGATCTTGTTGGTTTAGTCATCTCAATGCTTCTTTTAGGAGATTTTAGTTTAGTTTTGCTCACATTACTCCAACTGTATTCGTTTACATTAGTGGATGTCTTTCTGGTTCTGTTTATTTTACCGCTCGGCATTCTTCTCCCATTTCCTGCTGGTATTAATGCTCTGTTCAGTCATGGACCCAGACGTTCTGCCGGGCTTGCACGTATACATGCTCTGTGGAATCTTACATCCTTAATTAATGTTGTGGTTGCTTTCGTTTGTGGATATGTTCACTTTAACAGTCAAGCATCGAGTAAAAAACATCCATTTCAACCCTGGAATATAAGCATGGATGACAGTGAATGGTGGATTTTTCCTGCTGGTCTGGTTCTTTGTAAAATTTTTCAATCACAGCTAATCAATTGGCATGTAGCAAACCTGGAGATTCAAGACCGTTCCTTGTACAGCAATGATTTTGAGCTGTTCTGGCAATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1423

Amino Acids

152.65

Weight (kDa)

5.65

Isoelectric Point (pI)

47.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8003 PF26010 782 - 856 5.3e-36 Domain of unknown function (DUF8003)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2412
AasI GACNNNNNNGTC 2 cut(s) 920, 1158
AatII GACGTC 1 cut(s) 2089
Acc16I TGCGCA 1 cut(s) 1280
Acc65I GGTACC 2 cut(s) 1817, 3345
AccB1I GGYRCC 4 cut(s) 764, 1817, 3030, 3345
AccBSI CCGCTC 2 cut(s) 492, 3919
AccI GTMKAC 2 cut(s) 2901, 4000
AccII CGCG 5 cut(s) 518, 540, 545, 2669, 2671
AccIII TCCGGA 1 cut(s) 574
AclI AACGTT 1 cut(s) 351
AclWI GGATC 5 cut(s) 1537, 1713, 1726, 3322, 3804
AcoI YGGCCR 1 cut(s) 526
AcuI CTGAAG 5 cut(s) 903, 1251, 1653, 1655, 3600
AcvI CACGTG 3 cut(s) 228, 3113, 3590
AcyI GRCGYC 5 cut(s) 207, 597, 2086, 2516, 3031
AdeI CACNNNGTG 1 cut(s) 228
AfaI GTAC 8 cut(s) 979, 1819, 2131, 2978, 3347, 3474, 3717, 4238
AfiI CCNNNNNNNGG 9 cut(s) 178, 504, 606, 1687, 2096, 3096, 3187, 3254, 3355
AflIII ACRYGT 4 cut(s) 227, 264, 582, 3475
AhdI GACNNNNNGTC 1 cut(s) 2520
AhlI ACTAGT 1 cut(s) 3529
AjiI CACGTC 2 cut(s) 265, 583
AjuI GAANNNNNNNTTGG 2 cut(s) 2759, 2791
AloI GAACNNNNNNTCC 4 cut(s) 1710, 1742, 3193, 3225
Alw21I GWGCWC 2 cut(s) 2817, 3118
Alw26I GTCTC 8 cut(s) 244, 255, 2307, 2507, 2639, 3317, 3660, 3760
Alw44I GTGCAC 1 cut(s) 2813
AlwI GGATC 5 cut(s) 1537, 1713, 1726, 3322, 3804
Ama87I CYCGRG 1 cut(s) 2161
Aor13HI TCCGGA 1 cut(s) 574
AoxI GGCC 8 cut(s) 526, 623, 1523, 1543, 1705, 2193, 2603, 2704
ApaLI GTGCAC 1 cut(s) 2813
ApeKI GCWGC 5 cut(s) 616, 792, 1794, 1874, 3140
AseI ATTAAT 3 cut(s) 2937, 3951, 4032
Asp700I GAANNNNTTC 3 cut(s) 1047, 2843, 3066
Asp718I GGTACC 2 cut(s) 1817, 3345
AspLEI GCGC 7 cut(s) 418, 518, 540, 545, 1281, 2671, 3033
AsuC2I CCSGG 5 cut(s) 303, 1610, 1682, 2703, 3988
AsuHPI GGTGA 9 cut(s) 5, 247, 460, 718, 739, 767, 2065, 2078, 3269
AsuII TTCGAA 2 cut(s) 566, 1096
AsuNHI GCTAGC 1 cut(s) 3160
AvaI CYCGRG 1 cut(s) 2161
AvaII GGWCC 8 cut(s) 495, 559, 1460, 1746, 2379, 2834, 2909, 3971
AxyI CCTNAGG 1 cut(s) 3425
BaeGI GKGCMC 2 cut(s) 2440, 2817
BamHI GGATCC 1 cut(s) 1718
BanI GGYRCC 4 cut(s) 764, 1817, 3030, 3345
BauI CACGAG 1 cut(s) 604
BbrPI CACGTG 3 cut(s) 228, 3113, 3590
BbsI GAAGAC 6 cut(s) 99, 585, 1154, 2612, 3278, 3558
Bbv12I GWGCWC 2 cut(s) 2817, 3118
BbvCI CCTCAGC 2 cut(s) 747, 2793
BbvI GCAGC 5 cut(s) 603, 804, 1781, 1861, 3127
BccI CCATC 6 cut(s) 752, 1322, 2057, 2426, 3254, 3557
BceAI ACGGC 8 cut(s) 160, 454, 474, 529, 610, 2586, 3375, 3413
BciVI GTATCC 1 cut(s) 465
BcnI CCSGG 5 cut(s) 303, 1610, 1682, 2703, 3988
BcoDI GTCTC 8 cut(s) 244, 255, 2307, 2507, 2639, 3317, 3660, 3760
BcuI ACTAGT 1 cut(s) 3529
BfaI CTAG 5 cut(s) 72, 1107, 1371, 3161, 3530
BfmI CTRYAG 3 cut(s) 617, 1507, 3501
BfoI RGCGCY 1 cut(s) 3034
BfuI GTATCC 1 cut(s) 465
BglI GCCNNNNNGGC 1 cut(s) 3452
BglII AGATCT 1 cut(s) 2137
BlpI GCTNAGC 1 cut(s) 1181
Bme18I GGWCC 8 cut(s) 495, 559, 1460, 1746, 2379, 2834, 2909, 3971
BmeRI GACNNNNNGTC 1 cut(s) 2520
BmeT110I CYCGRG 1 cut(s) 2161
BmgBI CACGTC 2 cut(s) 265, 583
BmsI GCATC 8 cut(s) 95, 804, 979, 1308, 1790, 2869, 3694, 4091
BmtI GCTAGC 1 cut(s) 3164
BpiI GAAGAC 6 cut(s) 99, 585, 1154, 2612, 3278, 3558
BplI GAGNNNNNCTC 2 cut(s) 3396, 3428
BpmI CTGGAG 5 cut(s) 743, 2268, 2349, 2862, 4235
Bpu10I CCTNAGC 5 cut(s) 370, 682, 747, 1826, 2793
Bpu1102I GCTNAGC 1 cut(s) 1181
Bpu14I TTCGAA 2 cut(s) 566, 1096
BpuEI CTTGAG 1 cut(s) 1385
BpuMI CCSGG 5 cut(s) 303, 1610, 1682, 2703, 3988
BsaAI YACGTR 5 cut(s) 228, 3113, 3476, 3590, 3998
BsaBI GATNNNNATC 2 cut(s) 117, 2256
BsaHI GRCGYC 5 cut(s) 207, 597, 2086, 2516, 3031
BsaI GGTCTC 2 cut(s) 2307, 2639
BsaJI CCNNGG 9 cut(s) 199, 529, 2090, 2317, 2606, 2855, 3181, 3391, 4109
BsaWI WCCGGW 1 cut(s) 574
BsaXI ACNNNNNCTCC 4 cut(s) 681, 711, 3684, 3714
Bsc4I CCNNNNNNNGG 9 cut(s) 178, 504, 606, 1687, 2096, 3096, 3187, 3254, 3355
Bse118I RCCGGY 2 cut(s) 145, 2195
Bse1I ACTGG 5 cut(s) 826, 1771, 1804, 2251, 3652
Bse21I CCTNAGG 1 cut(s) 3425
Bse3DI GCAATG 8 cut(s) 952, 1648, 1749, 1837, 2265, 2979, 3673, 4249
Bse8I GATNNNNATC 2 cut(s) 117, 2256
BseAI TCCGGA 1 cut(s) 574
BseDI CCNNGG 9 cut(s) 199, 529, 2090, 2317, 2606, 2855, 3181, 3391, 4109
BseJI GATNNNNATC 2 cut(s) 117, 2256
BseLI CCNNNNNNNGG 9 cut(s) 178, 504, 606, 1687, 2096, 3096, 3187, 3254, 3355
BseMI GCAATG 8 cut(s) 952, 1648, 1749, 1837, 2265, 2979, 3673, 4249
BseMII CTCAG 5 cut(s) 456, 738, 824, 1402, 2784
BseNI ACTGG 5 cut(s) 826, 1771, 1804, 2251, 3652
BseRI GAGGAG 6 cut(s) 68, 1642, 2627, 2810, 2865, 3204
BseSI GKGCMC 2 cut(s) 2440, 2817
BseXI GCAGC 5 cut(s) 603, 804, 1781, 1861, 3127
BseYI CCCAGC 2 cut(s) 2168, 2707
BsgI GTGCAG 2 cut(s) 172, 1780
Bsh1236I CGCG 5 cut(s) 518, 540, 545, 2669, 2671
BshFI GGCC 8 cut(s) 528, 625, 1525, 1545, 1707, 2195, 2605, 2706
BshNI GGYRCC 4 cut(s) 764, 1817, 3030, 3345
BsiHKAI GWGCWC 2 cut(s) 2817, 3118
BsiHKCI CYCGRG 1 cut(s) 2161
BslFI GGGAC 6 cut(s) 508, 1103, 2317, 2353, 2392, 2785
BslI CCNNNNNNNGG 9 cut(s) 178, 504, 606, 1687, 2096, 3096, 3187, 3254, 3355
BsmAI GTCTC 8 cut(s) 244, 255, 2307, 2507, 2639, 3317, 3660, 3760
BsmBI CGTCTC 2 cut(s) 255, 2507
BsmFI GGGAC 6 cut(s) 508, 1103, 2317, 2353, 2392, 2785
BsmI GAATGC 3 cut(s) 35, 3373, 3924
BsnI GGCC 8 cut(s) 528, 625, 1525, 1545, 1707, 2195, 2605, 2706
Bso31I GGTCTC 2 cut(s) 2307, 2639
BsoBI CYCGRG 1 cut(s) 2161
Bsp119I TTCGAA 2 cut(s) 566, 1096
Bsp1286I GDGCHC 3 cut(s) 2440, 2817, 3118
Bsp13I TCCGGA 1 cut(s) 574
Bsp1407I TGTACA 3 cut(s) 2976, 3715, 4236
Bsp1720I GCTNAGC 1 cut(s) 1181
Bsp19I CCATGG 1 cut(s) 529
BspANI GGCC 8 cut(s) 528, 625, 1525, 1545, 1707, 2195, 2605, 2706
BspCNI CTCAG 5 cut(s) 455, 739, 823, 1401, 2785
BspEI TCCGGA 1 cut(s) 574
BspFNI CGCG 5 cut(s) 518, 540, 545, 2669, 2671
BspHI TCATGA 2 cut(s) 2446, 3315
BspMAI CTGCAG 1 cut(s) 621
BspOI GCTAGC 1 cut(s) 3164
BspPI GGATC 5 cut(s) 1537, 1713, 1726, 3322, 3804
BspT104I TTCGAA 2 cut(s) 566, 1096
BspT107I GGYRCC 4 cut(s) 764, 1817, 3030, 3345
BspTNI GGTCTC 2 cut(s) 2307, 2639
BsrBI CCGCTC 2 cut(s) 492, 3919
BsrDI GCAATG 8 cut(s) 952, 1648, 1749, 1837, 2265, 2979, 3673, 4249
BsrFI RCCGGY 2 cut(s) 145, 2195
BsrGI TGTACA 3 cut(s) 2976, 3715, 4236
BsrI ACTGG 5 cut(s) 826, 1771, 1804, 2251, 3652
BssAI RCCGGY 2 cut(s) 145, 2195
BssECI CCNNGG 9 cut(s) 199, 529, 2090, 2317, 2606, 2855, 3181, 3391, 4109
BssNAI GTATAC 2 cut(s) 2902, 4001
BssNI GRCGYC 5 cut(s) 207, 597, 2086, 2516, 3031
BssSI CACGAG 1 cut(s) 604
BssT1I CCWWGG 4 cut(s) 529, 2606, 2855, 3181
Bst1107I GTATAC 2 cut(s) 2902, 4001
Bst2BI CACGAG 1 cut(s) 604
Bst6I CTCTTC 5 cut(s) 30, 128, 874, 2126, 2574
BstACI GRCGYC 5 cut(s) 207, 597, 2086, 2516, 3031
BstAPI GCANNNNNTGC 2 cut(s) 971, 3055
BstAUI TGTACA 3 cut(s) 2976, 3715, 4236
BstBAI YACGTR 5 cut(s) 228, 3113, 3476, 3590, 3998
BstBI TTCGAA 2 cut(s) 566, 1096
BstDSI CCRYGG 2 cut(s) 529, 3391
BstFNI CGCG 5 cut(s) 518, 540, 545, 2669, 2671
BstH2I RGCGCY 1 cut(s) 3034
BstHHI GCGC 7 cut(s) 418, 518, 540, 545, 1281, 2671, 3033
BstMAI GTCTC 8 cut(s) 244, 255, 2307, 2507, 2639, 3317, 3660, 3760
BstNSI RCATGY 8 cut(s) 954, 1285, 1881, 2361, 2557, 3015, 4007, 4205
BstSFI CTRYAG 3 cut(s) 617, 1507, 3501
BstSLI GKGCMC 2 cut(s) 2440, 2817
BstUI CGCG 5 cut(s) 518, 540, 545, 2669, 2671
BstV1I GCAGC 5 cut(s) 603, 804, 1781, 1861, 3127
BstV2I GAAGAC 6 cut(s) 99, 585, 1154, 2612, 3278, 3558
BstX2I RGATCY 4 cut(s) 1529, 1718, 2137, 3796
BstXI CCANNNNNNTGG 3 cut(s) 1470, 1766, 3563
BstYI RGATCY 4 cut(s) 1529, 1718, 2137, 3796
BstZ17I GTATAC 2 cut(s) 2902, 4001
Bsu36I CCTNAGG 1 cut(s) 3425
BsuI GTATCC 1 cut(s) 465
BsuRI GGCC 8 cut(s) 528, 625, 1525, 1545, 1707, 2195, 2605, 2706
BtgI CCRYGG 2 cut(s) 529, 3391
BtrI CACGTC 2 cut(s) 265, 583
BtsI GCAGTG 1 cut(s) 1849
CciI TCATGA 2 cut(s) 2446, 3315
CfoI GCGC 7 cut(s) 418, 518, 540, 545, 1281, 2671, 3033
Cfr10I RCCGGY 2 cut(s) 145, 2195
CseI GACGC 3 cut(s) 196, 605, 2524
Csp6I GTAC 8 cut(s) 978, 1818, 2130, 2977, 3346, 3473, 3716, 4237
CviQI GTAC 8 cut(s) 978, 1818, 2130, 2977, 3346, 3473, 3716, 4237
DinI GGCGCC 1 cut(s) 3032
DraIII CACNNNGTG 1 cut(s) 228
DrdI GACNNNNNNGTC 2 cut(s) 920, 1158
DriI GACNNNNNGTC 1 cut(s) 2520
DseDI GACNNNNNNGTC 2 cut(s) 920, 1158
EaeI YGGCCR 1 cut(s) 526
Eam1104I CTCTTC 5 cut(s) 30, 128, 874, 2126, 2574
Eam1105I GACNNNNNGTC 1 cut(s) 2520
EarI CTCTTC 5 cut(s) 30, 128, 874, 2126, 2574
EciI GGCGGA 1 cut(s) 141
Eco130I CCWWGG 4 cut(s) 529, 2606, 2855, 3181
Eco147I AGGCCT 1 cut(s) 1707
Eco31I GGTCTC 2 cut(s) 2307, 2639
Eco32I GATATC 2 cut(s) 1693, 2549
Eco47I GGWCC 8 cut(s) 495, 559, 1460, 1746, 2379, 2834, 2909, 3971
Eco57I CTGAAG 5 cut(s) 903, 1251, 1653, 1655, 3600
Eco72I CACGTG 3 cut(s) 228, 3113, 3590
Eco81I CCTNAGG 1 cut(s) 3425
Eco88I CYCGRG 1 cut(s) 2161
EcoO109I RGGNCCY 2 cut(s) 495, 2379
EcoRI GAATTC 2 cut(s) 1309, 2217
EcoRV GATATC 2 cut(s) 1693, 2549
EcoT14I CCWWGG 4 cut(s) 529, 2606, 2855, 3181
EcoT22I ATGCAT 2 cut(s) 952, 2537
EgeI GGCGCC 1 cut(s) 3032
EheI GGCGCC 1 cut(s) 3032
ErhI CCWWGG 4 cut(s) 529, 2606, 2855, 3181
Esp3I CGTCTC 2 cut(s) 255, 2507
FaqI GGGAC 6 cut(s) 508, 1103, 2317, 2353, 2392, 2785
FauI CCCGC 5 cut(s) 180, 460, 485, 507, 511
FauNDI CATATG 2 cut(s) 1915, 3688
FblI GTMKAC 2 cut(s) 2901, 4000
FspBI CTAG 5 cut(s) 72, 1107, 1371, 3161, 3530
FspI TGCGCA 1 cut(s) 1280
GlaI GCGC 7 cut(s) 417, 517, 539, 544, 1280, 2670, 3032
GsaI CCCAGC 2 cut(s) 2172, 2711
GsuI CTGGAG 5 cut(s) 743, 2268, 2349, 2862, 4235
HaeII RGCGCY 1 cut(s) 3034
HaeIII GGCC 8 cut(s) 528, 625, 1525, 1545, 1707, 2195, 2605, 2706
HgaI GACGC 3 cut(s) 196, 605, 2524
HhaI GCGC 7 cut(s) 418, 518, 540, 545, 1281, 2671, 3033
Hin1I GRCGYC 5 cut(s) 207, 597, 2086, 2516, 3031
Hin6I GCGC 7 cut(s) 416, 516, 538, 543, 1279, 2669, 3031
HinP1I GCGC 7 cut(s) 416, 516, 538, 543, 1279, 2669, 3031
HincII GTYRAC 3 cut(s) 349, 1032, 1351
HindII GTYRAC 3 cut(s) 349, 1032, 1351
HindIII AAGCTT 1 cut(s) 569
HpaI GTTAAC 1 cut(s) 349
HphI GGTGA 9 cut(s) 5, 247, 460, 718, 739, 767, 2065, 2078, 3269
HpyAV CCTTC 6 cut(s) 927, 2039, 2477, 3119, 3425, 3672
Hsp92I GRCGYC 5 cut(s) 207, 597, 2086, 2516, 3031
HspAI GCGC 7 cut(s) 416, 516, 538, 543, 1279, 2669, 3031
KasI GGCGCC 1 cut(s) 3030
KflI GGGWCCC 1 cut(s) 495
Kpn2I TCCGGA 1 cut(s) 574
KpnI GGTACC 2 cut(s) 1821, 3349
KspAI GTTAAC 1 cut(s) 349
LmnI GCTCC 9 cut(s) 154, 341, 398, 639, 1271, 2716, 2969, 3280, 3566
Lsp1109I GCAGC 5 cut(s) 603, 804, 1781, 1861, 3127
LweI GCATC 8 cut(s) 95, 804, 979, 1308, 1790, 2869, 3694, 4091
MaeI CTAG 5 cut(s) 72, 1107, 1371, 3161, 3530
MaeIII GTNAC 9 cut(s) 374, 1169, 1373, 1443, 1573, 2053, 2344, 3304, 3406
MbiI CCGCTC 2 cut(s) 492, 3919
MfeI CAATTG 3 cut(s) 888, 3570, 4195
MflI RGATCY 4 cut(s) 1529, 1718, 2137, 3796
MhlI GDGCHC 3 cut(s) 2440, 2817, 3118
Mly113I GGCGCC 1 cut(s) 3031
MlyI GAGTC 5 cut(s) 1141, 1146, 1403, 2807, 3328
MmeI TCCRAC 2 cut(s) 3766, 3889
Mph1103I ATGCAT 2 cut(s) 952, 2537
MroI TCCGGA 1 cut(s) 574
MroXI GAANNNNTTC 3 cut(s) 1047, 2843, 3066
MslI CAYNNNNRTG 5 cut(s) 1413, 2808, 3016, 3561, 3818
MspA1I CMGCKG 3 cut(s) 1973, 2060, 2402
MteI GCGCNGCGC 1 cut(s) 541
MunI CAATTG 3 cut(s) 888, 3570, 4195
Mva1269I GAATGC 3 cut(s) 35, 3373, 3924
MvnI CGCG 5 cut(s) 518, 540, 545, 2669, 2671
NarI GGCGCC 1 cut(s) 3031
NciI CCSGG 5 cut(s) 303, 1610, 1682, 2703, 3988
NcoI CCATGG 1 cut(s) 529
NdeI CATATG 2 cut(s) 1915, 3688
NheI GCTAGC 1 cut(s) 3160
NmeAIII GCCGAG 3 cut(s) 180, 2536, 3900
NmuCI GTSAC 4 cut(s) 1169, 2053, 2344, 3304
NsbI TGCGCA 1 cut(s) 1280
NsiI ATGCAT 2 cut(s) 952, 2537
NspI RCATGY 8 cut(s) 954, 1285, 1881, 2361, 2557, 3015, 4007, 4205
NspV TTCGAA 2 cut(s) 566, 1096
PacI TTAATTAA 1 cut(s) 4032
PaeI GCATGC 2 cut(s) 2361, 3015
PaeR7I CTCGAG 1 cut(s) 2161
PagI TCATGA 2 cut(s) 2446, 3315
PceI AGGCCT 1 cut(s) 1707
PcsI WCGNNNNNNNCGW 2 cut(s) 3103, 3332
PctI GAATGC 3 cut(s) 35, 3373, 3924
PdmI GAANNNNTTC 3 cut(s) 1047, 2843, 3066
PleI GAGTC 5 cut(s) 1140, 1145, 1402, 2806, 3327
PluTI GGCGCC 1 cut(s) 3034
PmaCI CACGTG 3 cut(s) 228, 3113, 3590
PmlI CACGTG 3 cut(s) 228, 3113, 3590
PpsI GAGTC 5 cut(s) 1140, 1145, 1402, 2806, 3327
Ppu21I YACGTR 5 cut(s) 228, 3113, 3476, 3590, 3998
PpuMI RGGWCCY 2 cut(s) 495, 2379
PshBI ATTAAT 3 cut(s) 2937, 3951, 4032
PsiI TTATAA 1 cut(s) 2412
Psp1406I AACGTT 1 cut(s) 351
Psp5II RGGWCCY 2 cut(s) 495, 2379
PspCI CACGTG 3 cut(s) 228, 3113, 3590
PspFI CCCAGC 2 cut(s) 2168, 2707
PspPPI RGGWCCY 2 cut(s) 495, 2379
PstI CTGCAG 1 cut(s) 621
PsuI RGATCY 4 cut(s) 1529, 1718, 2137, 3796
PvuII CAGCTG 3 cut(s) 1973, 2060, 2402
RsaI GTAC 8 cut(s) 979, 1819, 2131, 2978, 3347, 3474, 3717, 4238
RsaNI GTAC 8 cut(s) 978, 1818, 2130, 2977, 3346, 3473, 3716, 4237
RseI CAYNNNNRTG 5 cut(s) 1413, 2808, 3016, 3561, 3818
SchI GAGTC 5 cut(s) 1141, 1146, 1403, 2807, 3328
SduI GDGCHC 3 cut(s) 2440, 2817, 3118
SfaNI GCATC 8 cut(s) 95, 804, 979, 1308, 1790, 2869, 3694, 4091
SfcI CTRYAG 3 cut(s) 617, 1507, 3501
SfoI GGCGCC 1 cut(s) 3032
Sfr274I CTCGAG 1 cut(s) 2161
SfuI TTCGAA 2 cut(s) 566, 1096
SinI GGWCC 8 cut(s) 495, 559, 1460, 1746, 2379, 2834, 2909, 3971
SlaI CTCGAG 1 cut(s) 2161
SmiMI CAYNNNNRTG 5 cut(s) 1413, 2808, 3016, 3561, 3818
SmlI CTYRAG 2 cut(s) 1364, 2161
SmoI CTYRAG 2 cut(s) 1364, 2161
SpeI ACTAGT 1 cut(s) 3529
SphI GCATGC 2 cut(s) 2361, 3015
SseBI AGGCCT 1 cut(s) 1707
SspDI GGCGCC 1 cut(s) 3030
SspI AATATT 2 cut(s) 1699, 3301
SspMI CTAG 5 cut(s) 72, 1107, 1371, 3161, 3530
StuI AGGCCT 1 cut(s) 1707
StyI CCWWGG 4 cut(s) 529, 2606, 2855, 3181
TatI WGTACW 3 cut(s) 2976, 3715, 4236
TseFI GTSAC 4 cut(s) 1169, 2053, 2344, 3304
TseI GCWGC 5 cut(s) 616, 792, 1794, 1874, 3140
Tsp45I GTSAC 4 cut(s) 1169, 2053, 2344, 3304
TspGWI ACGGA 1 cut(s) 1276
VneI GTGCAC 1 cut(s) 2813
VpaK11BI GGWCC 8 cut(s) 495, 559, 1460, 1746, 2379, 2834, 2909, 3971
VspI ATTAAT 3 cut(s) 2937, 3951, 4032
XceI RCATGY 8 cut(s) 954, 1285, 1881, 2361, 2557, 3015, 4007, 4205
XcmI CCANNNNNNNNNTGG 5 cut(s) 2272, 2324, 3339, 3800, 4107
XhoI CTCGAG 1 cut(s) 2161
XmiI GTMKAC 2 cut(s) 2901, 4000
XmnI GAANNNNTTC 3 cut(s) 1047, 2843, 3066
XspI CTAG 5 cut(s) 72, 1107, 1371, 3161, 3530
ZraI GACGTC 1 cut(s) 2087
Zsp2I ATGCAT 2 cut(s) 952, 2537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.