FvH4_1g15920

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003455
Physical Location & Seq
Reverse (-)
9053743 .. 9054622
880 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15920.t1

Sequence Viewer

Length: 297 bp
ATGGCCATAACGTGTTGTATTCAGGTGCAGCCTCACCAGGAGATGACAGTGGACGAGTTCAAAGCGTGGCTGCGCCGGTTCGACAATGATCACAATGGACAGATCAGCCGGGAGGAGCTAACAGAAGCCCTTCACAGCTTGAAGATTTGGTTCGGCTGGTGGAAAAGTCGTCGAGTCATGAAGCAAGCAGACTCCAAAAGAGATGGCCAAATAGACAACACCAAGGAATTCGAAAAGCTGGTAACCTATGCTCAGCAAAATCTGCACATGAAGATTTATCAAAGTAATGGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5

Protein Analysis

99

Amino Acids

11.8

Weight (kDa)

7.82

Isoelectric Point (pI)

48.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 19 - 46 8.2e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 3, 205
AcsI RAATTY 1 cut(s) 227
AflIII ACRYGT 1 cut(s) 11
AgsI TTSAA 2 cut(s) 61, 142
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 3 cut(s) 118, 138, 238
AluI AGCT 3 cut(s) 118, 138, 238
AoxI GGCC 2 cut(s) 3, 205
ApeKI GCWGC 2 cut(s) 28, 70
ApoI RAATTY 1 cut(s) 227
Asp700I GAANNNNTTC 1 cut(s) 129
AspLEI GCGC 1 cut(s) 75
AsuC2I CCSGG 1 cut(s) 110
AsuHPI GGTGA 1 cut(s) 26
AsuII TTCGAA 1 cut(s) 231
BalI TGGCCA 2 cut(s) 5, 207
BbvI GCAGC 2 cut(s) 40, 57
BccI CCATC 1 cut(s) 197
BciT130I CCWGG 1 cut(s) 38
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 1 cut(s) 110
BisI GCNGC 2 cut(s) 29, 71
BlpI GCTNAGC 1 cut(s) 252
BlsI GCNGC 2 cut(s) 30, 72
Bme1390I CCNGG 2 cut(s) 38, 110
BmrFI CCNGG 2 cut(s) 38, 110
Bpu1102I GCTNAGC 1 cut(s) 252
Bpu14I TTCGAA 1 cut(s) 231
BpuMI CCSGG 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 222
Bse118I RCCGGY 1 cut(s) 75
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 1 cut(s) 222
BseMII CTCAG 1 cut(s) 266
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 2 cut(s) 40, 57
BsgI GTGCAG 2 cut(s) 47, 248
BshFI GGCC 2 cut(s) 5, 207
BsiSI CCGG 2 cut(s) 76, 109
BsnI GGCC 2 cut(s) 5, 207
Bsp119I TTCGAA 1 cut(s) 231
Bsp143I GATC 2 cut(s) 88, 102
Bsp1720I GCTNAGC 1 cut(s) 252
BspANI GGCC 2 cut(s) 5, 207
BspCNI CTCAG 1 cut(s) 265
BspHI TCATGA 1 cut(s) 177
BspT104I TTCGAA 1 cut(s) 231
BsrFI RCCGGY 1 cut(s) 75
BssAI RCCGGY 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 2 cut(s) 88, 102
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 1 cut(s) 49
BstAPI GCANNNNNTGC 1 cut(s) 262
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 1 cut(s) 252
BstEII GGTNACC 1 cut(s) 241
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 2 cut(s) 91, 105
BstMBI GATC 2 cut(s) 88, 102
BstMWI GCNNNNNNNGC 1 cut(s) 262
BstNI CCWGG 1 cut(s) 38
BstPI GGTNACC 1 cut(s) 241
BstSCI CCNGG 2 cut(s) 36, 108
BstV1I GCAGC 2 cut(s) 40, 57
BsuRI GGCC 2 cut(s) 5, 207
BtsIMutI CAGTG 1 cut(s) 54
Cac8I GCNNGC 1 cut(s) 186
CciI TCATGA 1 cut(s) 177
CfoI GCGC 1 cut(s) 75
Cfr10I RCCGGY 1 cut(s) 75
CviAII CATG 2 cut(s) 178, 268
DdeI CTNAG 1 cut(s) 252
DpnI GATC 2 cut(s) 90, 104
DpnII GATC 2 cut(s) 88, 102
EaeI YGGCCR 2 cut(s) 3, 205
Eco130I CCWWGG 1 cut(s) 222
Eco91I GGTNACC 1 cut(s) 241
EcoO65I GGTNACC 1 cut(s) 241
EcoRI GAATTC 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 2 cut(s) 181, 271
FaiI YATR 4 cut(s) 8, 179, 249, 269
FatI CATG 2 cut(s) 177, 267
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 2 cut(s) 29, 71
Fsp4HI GCNGC 2 cut(s) 29, 71
GlaI GCGC 1 cut(s) 74
GluI GCNGC 2 cut(s) 29, 71
HaeIII GGCC 2 cut(s) 5, 207
HapII CCGG 2 cut(s) 76, 109
HhaI GCGC 1 cut(s) 75
Hin1II CATG 2 cut(s) 181, 271
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HinfI GANTC 2 cut(s) 174, 191
HpaII CCGG 2 cut(s) 76, 109
HphI GGTGA 1 cut(s) 26
Hpy166II GTNNAC 1 cut(s) 52
Hpy188III TCNNGA 1 cut(s) 178
Hpy8I GTNNAC 1 cut(s) 52
Hpy99I CGWCG 1 cut(s) 174
HpyAV CCTTC 1 cut(s) 140
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 2 cut(s) 28, 265
HpyF10VI GCNNNNNNNGC 1 cut(s) 262
HpyF3I CTNAG 1 cut(s) 252
HpySE526I ACGT 1 cut(s) 11
Hsp92II CATG 2 cut(s) 181, 271
HspAI GCGC 1 cut(s) 73
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 2 cut(s) 88, 102
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 7 cut(s) 8, 23, 50, 89, 122, 142, 224
Lsp1109I GCAGC 2 cut(s) 40, 57
MaeII ACGT 1 cut(s) 11
MaeIII GTNAC 1 cut(s) 241
MalI GATC 2 cut(s) 90, 104
MboI GATC 2 cut(s) 88, 102
MboII GAAGA 2 cut(s) 154, 283
MlsI TGGCCA 2 cut(s) 5, 207
MluCI AATT 1 cut(s) 227
MluNI TGGCCA 2 cut(s) 5, 207
MlyI GAGTC 2 cut(s) 183, 185
MnlI CCTC 2 cut(s) 42, 106
Mox20I TGGCCA 2 cut(s) 5, 207
MroXI GAANNNNTTC 1 cut(s) 129
MscI TGGCCA 2 cut(s) 5, 207
Msp20I TGGCCA 2 cut(s) 5, 207
MspI CCGG 2 cut(s) 76, 109
MspR9I CCNGG 2 cut(s) 38, 110
MvaI CCWGG 1 cut(s) 38
MwoI GCNNNNNNNGC 1 cut(s) 262
NciI CCSGG 1 cut(s) 110
NdeII GATC 2 cut(s) 88, 102
NlaIII CATG 2 cut(s) 181, 271
NspV TTCGAA 1 cut(s) 231
PagI TCATGA 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 129
PkrI GCNGC 2 cut(s) 30, 72
PleI GAGTC 2 cut(s) 182, 185
PpsI GAGTC 2 cut(s) 182, 185
Psp6I CCWGG 1 cut(s) 36
PspEI GGTNACC 1 cut(s) 241
PspGI CCWGG 1 cut(s) 36
SatI GCNGC 2 cut(s) 29, 71
Sau3AI GATC 2 cut(s) 88, 102
SchI GAGTC 2 cut(s) 183, 185
ScrFI CCNGG 2 cut(s) 38, 110
SetI ASST 6 cut(s) 14, 27, 120, 140, 240, 248
SfuI TTCGAA 1 cut(s) 231
Sse9I AATT 1 cut(s) 227
StyD4I CCNGG 2 cut(s) 36, 108
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 49
TaiI ACGT 1 cut(s) 14
TaqI TCGA 3 cut(s) 81, 172, 231
TasI AATT 1 cut(s) 227
TscAI CASTG 1 cut(s) 54
TseI GCWGC 2 cut(s) 28, 70
TspDTI ATGAA 2 cut(s) 194, 284
TspRI CASTG 1 cut(s) 54
XapI RAATTY 1 cut(s) 227
XmnI GAANNNNTTC 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.