RLG00000017415

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003455
Physical Location & Seq
Reverse (-)
16353268 .. 16356368
3101 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017415

Sequence Viewer

Length: 732 bp
ATGGAGGAGATAAGATGGGTGTTTGATAAATTTGACACCAACAAAGACGGGAAGATAAGCCGGGAGGAGTACAAGTCGGCGTTGAAAACCCTCGACAAAGAAATGACAGAAGCTGAGATTGCCAAGACATTCAAGGCCCTTGATTCTGACGGAGACGGGTCCATAGGCTGGACGGAGTTTGTGGAGATGTTCAACATGGGAGCTGATGAGGCCAAGAAAGCTGACATTGAAGGAGCTTTTCGGGTGTTTGATTTGGATGGAAATGGGAAGATAAGCGCAGAGGAGTTGTCTCTTGTTCTCAAGAAGCTGGGAGAGAATTTCAGCCTTGGGGCTTGTAAGAAAATGGTGAAAGGGGTGGATGTTGATGGTGATGGTTTGATAGATATAAATGAGTTTACCAAAATGATGGGAAGCTTCAAGAAACCAATGTTAAATGCAGAGTCTCCTTCTCTAGTCCAAGTGCAGCCTCACCAGGAGATGACAGTGGACGAGTTCAAAGCGTGGCTGCGCCGGTTCGACAATGATCACAATGGACAGATCAGCCGGGAGGAGCTAAAAGAAGCTCTTCACAGCTTGAGGATTTGGTTCGGGTGGTGGAGAAGTCGTCAAGTAATGAAGCAAGCAGACTTCAATAGAGATGGCCAAATAGACAACGCCAAGGAATTCGAAAAGCTGGTGAACTATGCTCAGCAAAATTTGCACATGAAGATTTATGAGAGTAATGGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5

Protein Analysis

244

Amino Acids

28.0

Weight (kDa)

5.07

Isoelectric Point (pI)

33.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 2 - 64 7.3e-18 EF-hand domain pair
EF-hand_1 PF00036 3 - 31 1.7e-08 EF hand domain
EF-hand_5 PF13202 7 - 26 3e-07 EF hand
EF-hand_6 PF13405 7 - 31 4e-06 EF-hand domain
EF-hand_8 PF13833 26 - 66 6.7e-07 EF-hand domain pair
EF-hand_7 PF13499 74 - 136 1.7e-12 EF-hand domain pair
EF-hand_1 PF00036 76 - 103 5.8e-07 EF hand domain
EF-hand_6 PF13405 76 - 104 1.4e-06 EF-hand domain
EF-hand_5 PF13202 79 - 98 1.2e-06 EF hand
EF-hand_1 PF00036 164 - 191 9.1e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 168
AcoI YGGCCR 1 cut(s) 640
AcsI RAATTY 4 cut(s) 29, 316, 662, 694
AfaI GTAC 1 cut(s) 71
AfiI CCNNNNNNNGG 1 cut(s) 168
AgsI TTSAA 7 cut(s) 85, 133, 193, 230, 418, 496, 631
AjnI CCWGG 1 cut(s) 471
Alw26I GTCTC 3 cut(s) 147, 294, 447
AlwNI CAGNNNCTG 1 cut(s) 113
AoxI GGCC 3 cut(s) 135, 210, 640
ApeKI GCWGC 2 cut(s) 463, 505
ApoI RAATTY 4 cut(s) 29, 316, 662, 694
Asp700I GAANNNNTTC 1 cut(s) 564
AspLEI GCGC 2 cut(s) 278, 510
AspS9I GGNCC 2 cut(s) 136, 159
AsuC2I CCSGG 2 cut(s) 62, 545
AsuHPI GGTGA 4 cut(s) 358, 380, 461, 688
AsuII TTCGAA 1 cut(s) 666
AvaII GGWCC 1 cut(s) 159
BalI TGGCCA 1 cut(s) 642
BbvI GCAGC 2 cut(s) 475, 492
BccI CCATC 6 cut(s) 9, 251, 359, 365, 400, 632
BciT130I CCWGG 1 cut(s) 473
BclI TGATCA 1 cut(s) 523
BcnI CCSGG 2 cut(s) 62, 545
BcoDI GTCTC 3 cut(s) 147, 294, 447
BfaI CTAG 1 cut(s) 452
BisI GCNGC 2 cut(s) 464, 506
BlpI GCTNAGC 1 cut(s) 687
BlsI GCNGC 2 cut(s) 465, 507
Bme1390I CCNGG 3 cut(s) 62, 473, 545
Bme18I GGWCC 1 cut(s) 159
BmgT120I GGNCC 2 cut(s) 136, 159
BmiI GGNNCC 1 cut(s) 160
BmrFI CCNGG 3 cut(s) 62, 473, 545
Bpu1102I GCTNAGC 1 cut(s) 687
Bpu14I TTCGAA 1 cut(s) 666
BpuEI CTTGAG 2 cut(s) 284, 595
BpuMI CCSGG 2 cut(s) 62, 545
BsaJI CCNNGG 2 cut(s) 325, 657
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 589, 619
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse118I RCCGGY 1 cut(s) 510
BseBI CCWGG 1 cut(s) 473
BseDI CCNNGG 2 cut(s) 325, 657
BseGI GGATG 2 cut(s) 262, 364
BseLI CCNNNNNNNGG 1 cut(s) 168
BseMII CTCAG 2 cut(s) 105, 701
BseRI GAGGAG 4 cut(s) 20, 80, 296, 563
BseXI GCAGC 2 cut(s) 475, 492
BseYI CCCAGC 1 cut(s) 307
BsgI GTGCAG 1 cut(s) 482
BshFI GGCC 3 cut(s) 137, 212, 642
BsiSI CCGG 3 cut(s) 61, 511, 544
BslI CCNNNNNNNGG 1 cut(s) 168
BsmAI GTCTC 3 cut(s) 147, 294, 447
BsmBI CGTCTC 1 cut(s) 147
BsnI GGCC 3 cut(s) 137, 212, 642
Bsp119I TTCGAA 1 cut(s) 666
Bsp143I GATC 2 cut(s) 523, 537
Bsp1720I GCTNAGC 1 cut(s) 687
BspANI GGCC 3 cut(s) 137, 212, 642
BspCNI CTCAG 2 cut(s) 106, 700
BspLI GGNNCC 1 cut(s) 160
BspQI GCTCTTC 1 cut(s) 570
BspT104I TTCGAA 1 cut(s) 666
BsrFI RCCGGY 1 cut(s) 510
BssAI RCCGGY 1 cut(s) 510
BssECI CCNNGG 2 cut(s) 325, 657
BssMI GATC 2 cut(s) 523, 537
BssT1I CCWWGG 2 cut(s) 325, 657
Bst2UI CCWGG 1 cut(s) 473
Bst4CI ACNGT 1 cut(s) 484
Bst6I CTCTTC 1 cut(s) 570
BstAPI GCANNNNNTGC 1 cut(s) 697
BstBI TTCGAA 1 cut(s) 666
BstC8I GCNNGC 1 cut(s) 621
BstDEI CTNAG 2 cut(s) 114, 687
BstF5I GGATG 2 cut(s) 262, 364
BstHHI GCGC 2 cut(s) 278, 510
BstKTI GATC 2 cut(s) 526, 540
BstMAI GTCTC 3 cut(s) 147, 294, 447
BstMBI GATC 2 cut(s) 523, 537
BstMWI GCNNNNNNNGC 4 cut(s) 119, 209, 218, 697
BstNI CCWGG 1 cut(s) 473
BstSCI CCNGG 3 cut(s) 60, 471, 543
BstV1I GCAGC 2 cut(s) 475, 492
BstXI CCANNNNNNTGG 1 cut(s) 406
BsuRI GGCC 3 cut(s) 137, 212, 642
BtsCI GGATG 2 cut(s) 262, 364
BtsIMutI CAGTG 1 cut(s) 489
Cac8I GCNNGC 1 cut(s) 621
CaiI CAGNNNCTG 1 cut(s) 113
CfoI GCGC 2 cut(s) 278, 510
Cfr10I RCCGGY 1 cut(s) 510
Cfr13I GGNCC 2 cut(s) 136, 159
Csp6I GTAC 1 cut(s) 70
CviAII CATG 2 cut(s) 196, 703
CviQI GTAC 1 cut(s) 70
DdeI CTNAG 2 cut(s) 114, 687
DpnI GATC 2 cut(s) 525, 539
DpnII GATC 2 cut(s) 523, 537
EaeI YGGCCR 1 cut(s) 640
Eam1104I CTCTTC 1 cut(s) 570
EarI CTCTTC 1 cut(s) 570
Eco130I CCWWGG 2 cut(s) 325, 657
Eco47I GGWCC 1 cut(s) 159
EcoO109I RGGNCCY 1 cut(s) 136
EcoRI GAATTC 1 cut(s) 662
EcoRII CCWGG 1 cut(s) 471
EcoT14I CCWWGG 2 cut(s) 325, 657
ErhI CCWWGG 2 cut(s) 325, 657
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 2 cut(s) 199, 706
FaiI YATR 6 cut(s) 164, 197, 386, 684, 704, 714
FalI AAGNNNNNCTT 2 cut(s) 549, 581
FatI CATG 2 cut(s) 195, 702
FbaI TGATCA 1 cut(s) 523
Fnu4HI GCNGC 2 cut(s) 464, 506
FokI GGATG 2 cut(s) 269, 371
Fsp4HI GCNGC 2 cut(s) 464, 506
FspBI CTAG 1 cut(s) 452
GlaI GCGC 2 cut(s) 277, 509
GluI GCNGC 2 cut(s) 464, 506
GsaI CCCAGC 1 cut(s) 311
HaeIII GGCC 3 cut(s) 137, 212, 642
HapII CCGG 3 cut(s) 61, 511, 544
HhaI GCGC 2 cut(s) 278, 510
Hin1II CATG 2 cut(s) 199, 706
Hin6I GCGC 2 cut(s) 276, 508
HinP1I GCGC 2 cut(s) 276, 508
HindIII AAGCTT 1 cut(s) 412
HinfI GANTC 2 cut(s) 143, 440
HpaII CCGG 3 cut(s) 61, 511, 544
HphI GGTGA 4 cut(s) 358, 380, 461, 688
Hpy166II GTNNAC 3 cut(s) 396, 487, 679
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 2 cut(s) 301, 418
Hpy8I GTNNAC 3 cut(s) 396, 487, 679
HpyAV CCTTC 2 cut(s) 224, 456
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4V TGCA 3 cut(s) 437, 463, 700
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 209, 218, 697
HpyF3I CTNAG 2 cut(s) 114, 687
Hsp92II CATG 2 cut(s) 199, 706
HspAI GCGC 2 cut(s) 276, 508
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 2 cut(s) 523, 537
LguI GCTCTTC 1 cut(s) 570
LmnI GCTCC 3 cut(s) 200, 233, 550
LpnPI CCDG 8 cut(s) 74, 154, 293, 458, 485, 524, 557, 659
Lsp1109I GCAGC 2 cut(s) 475, 492
MaeI CTAG 1 cut(s) 452
MalI GATC 2 cut(s) 525, 539
MboI GATC 2 cut(s) 523, 537
MboII GAAGA 4 cut(s) 64, 280, 557, 718
MlsI TGGCCA 1 cut(s) 642
MluCI AATT 4 cut(s) 29, 316, 662, 694
MluNI TGGCCA 1 cut(s) 642
MlyI GAGTC 1 cut(s) 449
MnlI CCTC 7 cut(s) 58, 101, 202, 274, 477, 541, 570
Mox20I TGGCCA 1 cut(s) 642
MroXI GAANNNNTTC 1 cut(s) 564
MscI TGGCCA 1 cut(s) 642
MseI TTAA 1 cut(s) 431
Msp20I TGGCCA 1 cut(s) 642
MspI CCGG 3 cut(s) 61, 511, 544
MspR9I CCNGG 3 cut(s) 62, 473, 545
MvaI CCWGG 1 cut(s) 473
MwoI GCNNNNNNNGC 4 cut(s) 119, 209, 218, 697
NciI CCSGG 2 cut(s) 62, 545
NdeII GATC 2 cut(s) 523, 537
NlaIII CATG 2 cut(s) 199, 706
NlaIV GGNNCC 1 cut(s) 160
NspV TTCGAA 1 cut(s) 666
PciSI GCTCTTC 1 cut(s) 570
PdmI GAANNNNTTC 1 cut(s) 564
PfeI GAWTC 1 cut(s) 143
PflMI CCANNNNNTGG 1 cut(s) 168
PkrI GCNGC 2 cut(s) 465, 507
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
Psp6I CCWGG 1 cut(s) 471
PspFI CCCAGC 1 cut(s) 307
PspGI CCWGG 1 cut(s) 471
PspN4I GGNNCC 1 cut(s) 160
PspPI GGNCC 2 cut(s) 136, 159
PstNI CAGNNNCTG 1 cut(s) 113
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
SapI GCTCTTC 1 cut(s) 570
SaqAI TTAA 1 cut(s) 431
SatI GCNGC 2 cut(s) 464, 506
Sau3AI GATC 2 cut(s) 523, 537
Sau96I GGNCC 2 cut(s) 136, 159
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 3 cut(s) 62, 473, 545
SfuI TTCGAA 1 cut(s) 666
SinI GGWCC 1 cut(s) 159
SmlI CTYRAG 2 cut(s) 299, 574
SmoI CTYRAG 2 cut(s) 299, 574
Sse9I AATT 4 cut(s) 29, 316, 662, 694
SspMI CTAG 1 cut(s) 452
StyD4I CCNGG 3 cut(s) 60, 471, 543
StyI CCWWGG 2 cut(s) 325, 657
TaaI ACNGT 1 cut(s) 484
TaqI TCGA 3 cut(s) 93, 516, 666
TasI AATT 4 cut(s) 29, 316, 662, 694
TatI WGTACW 1 cut(s) 69
TfiI GAWTC 1 cut(s) 143
Tru1I TTAA 1 cut(s) 431
Tru9I TTAA 1 cut(s) 431
TscAI CASTG 1 cut(s) 489
TseI GCWGC 2 cut(s) 463, 505
TspDTI ATGAA 2 cut(s) 629, 719
TspGWI ACGGA 2 cut(s) 165, 188
TspRI CASTG 1 cut(s) 489
Van91I CCANNNNNTGG 1 cut(s) 168
VpaK11BI GGWCC 1 cut(s) 159
XapI RAATTY 4 cut(s) 29, 316, 662, 694
XmnI GAANNNNTTC 1 cut(s) 564
XspI CTAG 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.