pycom15g24590

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003455
Physical Location & Seq
Reverse (-)
18793154 .. 18793450
297 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g24590.1

Sequence Viewer

Length: 297 bp
ATGGCCATAATGTGTTGTGTGCAGGTGCAGCCTAACCAGGAGATGACGGTGGAAGAGTTCAAAGCGTGGATTTACGGATTTGACAATGATCACAACGGGCAGATTAGCCGTGAGGAACTGAAAGAGGCTCTTCGCAGCCTGAGGGTTTGGTTCGGGTGGTGGAGAGCTCGCCAAGCAATTAAGTCATCGGATTTCAACGGTAGTGGCCAAATCGACAACCCCAAAGAGTTTGAGAAGCTTGTTAACTATGCACAGCAACGTCTAGGCATGAAAATTCATGAAAATAATGATTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5

Protein Analysis

99

Amino Acids

11.64

Weight (kDa)

5.64

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 13
AcoI YGGCCR 2 cut(s) 3, 205
AcsI RAATTY 1 cut(s) 273
AgsI TTSAA 2 cut(s) 61, 196
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 2 cut(s) 167, 238
AluI AGCT 2 cut(s) 167, 238
Alw21I GWGCWC 1 cut(s) 169
AoxI GGCC 2 cut(s) 3, 205
ApeKI GCWGC 2 cut(s) 28, 135
ApoI RAATTY 1 cut(s) 273
AxyI CCTNAGG 1 cut(s) 140
BalI TGGCCA 2 cut(s) 5, 207
BanII GRGCYC 1 cut(s) 169
Bbv12I GWGCWC 1 cut(s) 169
BbvI GCAGC 2 cut(s) 40, 147
BceAI ACGGC 1 cut(s) 93
BciT130I CCWGG 1 cut(s) 38
BclI TGATCA 1 cut(s) 88
BfaI CTAG 1 cut(s) 263
BfuAI ACCTGC 1 cut(s) 13
BisI GCNGC 2 cut(s) 29, 136
BlsI GCNGC 2 cut(s) 30, 137
Bme1390I CCNGG 1 cut(s) 38
BmrFI CCNGG 1 cut(s) 38
Bse21I CCTNAGG 1 cut(s) 140
BseBI CCWGG 1 cut(s) 38
BseMII CTCAG 1 cut(s) 131
BseXI GCAGC 2 cut(s) 40, 147
BsgI GTGCAG 2 cut(s) 41, 47
BshFI GGCC 2 cut(s) 5, 207
BsiHKAI GWGCWC 1 cut(s) 169
BsnI GGCC 2 cut(s) 5, 207
Bsp1286I GDGCHC 1 cut(s) 169
Bsp143I GATC 1 cut(s) 88
BspANI GGCC 2 cut(s) 5, 207
BspCNI CTCAG 1 cut(s) 132
BspHI TCATGA 1 cut(s) 277
BspMI ACCTGC 1 cut(s) 13
BspQI GCTCTTC 1 cut(s) 135
BssMI GATC 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 2 cut(s) 49, 200
Bst6I CTCTTC 2 cut(s) 48, 135
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 1 cut(s) 140
BstKTI GATC 1 cut(s) 91
BstMBI GATC 1 cut(s) 88
BstMWI GCNNNNNNNGC 2 cut(s) 28, 173
BstNI CCWGG 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 36
BstV1I GCAGC 2 cut(s) 40, 147
Bsu36I CCTNAGG 1 cut(s) 140
BsuRI GGCC 2 cut(s) 5, 207
BveI ACCTGC 1 cut(s) 13
Cac8I GCNNGC 1 cut(s) 169
CciI TCATGA 1 cut(s) 277
CspCI CAANNNNNGTGG 2 cut(s) 184, 219
CviAII CATG 2 cut(s) 268, 278
CviJI RGCY 8 cut(s) 5, 31, 108, 128, 138, 167, 207, 238
CviKI_1 RGCY 8 cut(s) 5, 31, 108, 128, 138, 167, 207, 238
DdeI CTNAG 1 cut(s) 140
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
EaeI YGGCCR 2 cut(s) 3, 205
Eam1104I CTCTTC 2 cut(s) 48, 135
EarI CTCTTC 2 cut(s) 48, 135
Ecl136II GAGCTC 1 cut(s) 167
Eco24I GRGCYC 1 cut(s) 169
Eco53kI GAGCTC 1 cut(s) 167
Eco81I CCTNAGG 1 cut(s) 140
EcoICRI GAGCTC 1 cut(s) 167
EcoRII CCWGG 1 cut(s) 36
EcoT38I GRGCYC 1 cut(s) 169
FaeI CATG 2 cut(s) 271, 281
FaiI YATR 4 cut(s) 8, 249, 269, 279
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FatI CATG 2 cut(s) 267, 277
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 2 cut(s) 29, 136
FriOI GRGCYC 1 cut(s) 169
Fsp4HI GCNGC 2 cut(s) 29, 136
FspBI CTAG 1 cut(s) 263
GluI GCNGC 2 cut(s) 29, 136
HaeIII GGCC 2 cut(s) 5, 207
Hin1II CATG 2 cut(s) 271, 281
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 1 cut(s) 236
HpaI GTTAAC 1 cut(s) 244
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 1 cut(s) 190
Hpy188III TCNNGA 1 cut(s) 278
Hpy8I GTNNAC 1 cut(s) 244
HpyCH4III ACNGT 2 cut(s) 49, 200
HpyCH4IV ACGT 1 cut(s) 259
HpyCH4V TGCA 3 cut(s) 22, 28, 251
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 173
HpyF3I CTNAG 1 cut(s) 140
HpySE526I ACGT 1 cut(s) 259
Hsp92II CATG 2 cut(s) 271, 281
Ksp22I TGATCA 1 cut(s) 88
KspAI GTTAAC 1 cut(s) 244
Kzo9I GATC 1 cut(s) 88
LguI GCTCTTC 1 cut(s) 135
LpnPI CCDG 4 cut(s) 8, 23, 50, 152
Lsp1109I GCAGC 2 cut(s) 40, 147
MaeI CTAG 1 cut(s) 263
MaeII ACGT 1 cut(s) 259
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MboII GAAGA 2 cut(s) 65, 122
MhlI GDGCHC 1 cut(s) 169
MlsI TGGCCA 2 cut(s) 5, 207
MluCI AATT 2 cut(s) 177, 273
MluNI TGGCCA 2 cut(s) 5, 207
MnlI CCTC 3 cut(s) 106, 118, 135
Mox20I TGGCCA 2 cut(s) 5, 207
MscI TGGCCA 2 cut(s) 5, 207
MseI TTAA 2 cut(s) 180, 243
Msp20I TGGCCA 2 cut(s) 5, 207
MspR9I CCNGG 1 cut(s) 38
MvaI CCWGG 1 cut(s) 38
MwoI GCNNNNNNNGC 2 cut(s) 28, 173
NdeII GATC 1 cut(s) 88
NlaIII CATG 2 cut(s) 271, 281
PagI TCATGA 1 cut(s) 277
PaqCI CACCTGC 1 cut(s) 13
PciSI GCTCTTC 1 cut(s) 135
PkrI GCNGC 2 cut(s) 30, 137
Psp124BI GAGCTC 1 cut(s) 169
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
SacI GAGCTC 1 cut(s) 169
SapI GCTCTTC 1 cut(s) 135
SaqAI TTAA 2 cut(s) 180, 243
SatI GCNGC 2 cut(s) 29, 136
Sau3AI GATC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 1 cut(s) 169
SetI ASST 4 cut(s) 27, 169, 240, 262
Sse9I AATT 2 cut(s) 177, 273
SspMI CTAG 1 cut(s) 263
SstI GAGCTC 1 cut(s) 169
StyD4I CCNGG 1 cut(s) 36
TaaI ACNGT 2 cut(s) 49, 200
TaiI ACGT 1 cut(s) 262
TaqI TCGA 1 cut(s) 213
TasI AATT 2 cut(s) 177, 273
Tru1I TTAA 2 cut(s) 180, 243
Tru9I TTAA 2 cut(s) 180, 243
TseI GCWGC 2 cut(s) 28, 135
TspDTI ATGAA 3 cut(s) 266, 284, 294
TspGWI ACGGA 1 cut(s) 90
XapI RAATTY 1 cut(s) 273
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.