Prupe.7G134600_v2.0.a1

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0003455
Physical Location & Seq
Forward (+)
15241359 .. 15242172
814 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G134600.1

Sequence Viewer

Length: 297 bp
ATGGCCATAGTAAGTTGTTTGCAGGTGCAGCCCGACCAGGAGATGACAGTGGATGAGTTCAAAGCGTGGCTTCACCGGTTTGACAGCGATCACAACGGGCAAATTAGCCGCGAAGAGCTGAAAGAAGCTCTCCACAGCCTGAGGGTTTGGTTTGGATGGTGGAGGGCTCGTCAAGGAATGAAGCAAGCAGATTGCAATAAGAGTGGCCAAATAGACAACCCCAAAGAGTTTGAAAAGCTGGTGAGCTATGCACAGCAGCGTTTGGGCATGAAGATTCTTGAAAATAATTGTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5

Protein Analysis

99

Amino Acids

11.61

Weight (kDa)

6.27

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 13
Acc36I ACCTGC 1 cut(s) 13
AccII CGCG 1 cut(s) 111
AciI CCGC 1 cut(s) 109
AcoI YGGCCR 2 cut(s) 3, 205
AgeI ACCGGT 1 cut(s) 75
AgsI TTSAA 3 cut(s) 61, 233, 281
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 4 cut(s) 118, 128, 238, 246
AluI AGCT 4 cut(s) 118, 128, 238, 246
AoxI GGCC 2 cut(s) 3, 205
ApeKI GCWGC 2 cut(s) 28, 256
AsiGI ACCGGT 1 cut(s) 75
AsuHPI GGTGA 2 cut(s) 65, 253
AxyI CCTNAGG 1 cut(s) 140
BalI TGGCCA 2 cut(s) 5, 207
BanII GRGCYC 1 cut(s) 169
BbvI GCAGC 2 cut(s) 40, 268
BccI CCATC 1 cut(s) 150
BciT130I CCWGG 1 cut(s) 38
BfuAI ACCTGC 1 cut(s) 13
BisI GCNGC 3 cut(s) 29, 109, 257
BlsI GCNGC 3 cut(s) 30, 110, 258
Bme1390I CCNGG 1 cut(s) 38
BmrFI CCNGG 1 cut(s) 38
BsaWI WCCGGW 1 cut(s) 75
BsaXI ACNNNNNCTCC 2 cut(s) 154, 184
Bse118I RCCGGY 1 cut(s) 75
Bse21I CCTNAGG 1 cut(s) 140
BseBI CCWGG 1 cut(s) 38
BseGI GGATG 2 cut(s) 58, 161
BseMII CTCAG 1 cut(s) 131
BseXI GCAGC 2 cut(s) 40, 268
BsgI GTGCAG 1 cut(s) 47
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 2 cut(s) 5, 207
BshTI ACCGGT 1 cut(s) 75
BsiSI CCGG 1 cut(s) 76
BsnI GGCC 2 cut(s) 5, 207
Bsp1286I GDGCHC 1 cut(s) 169
Bsp143I GATC 1 cut(s) 88
BspACI CCGC 1 cut(s) 109
BspANI GGCC 2 cut(s) 5, 207
BspCNI CTCAG 1 cut(s) 132
BspFNI CGCG 1 cut(s) 111
BspMI ACCTGC 1 cut(s) 13
BspQI GCTCTTC 1 cut(s) 108
BsrFI RCCGGY 1 cut(s) 75
BssAI RCCGGY 1 cut(s) 75
BssMI GATC 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 1 cut(s) 49
Bst6I CTCTTC 1 cut(s) 108
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 1 cut(s) 140
BstF5I GGATG 2 cut(s) 58, 161
BstFNI CGCG 1 cut(s) 111
BstKTI GATC 1 cut(s) 91
BstMBI GATC 1 cut(s) 88
BstMWI GCNNNNNNNGC 1 cut(s) 28
BstNI CCWGG 1 cut(s) 38
BstSCI CCNGG 1 cut(s) 36
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 2 cut(s) 40, 268
Bsu36I CCTNAGG 1 cut(s) 140
BsuRI GGCC 2 cut(s) 5, 207
BtsCI GGATG 2 cut(s) 58, 161
BtsIMutI CAGTG 1 cut(s) 54
BveI ACCTGC 1 cut(s) 13
Cac8I GCNNGC 1 cut(s) 186
Cfr10I RCCGGY 1 cut(s) 75
CspAI ACCGGT 1 cut(s) 75
CspCI CAANNNNNGTGG 2 cut(s) 184, 219
CviAII CATG 1 cut(s) 268
DdeI CTNAG 1 cut(s) 140
DpnI GATC 1 cut(s) 90
DpnII GATC 1 cut(s) 88
EaeI YGGCCR 2 cut(s) 3, 205
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
Eco24I GRGCYC 1 cut(s) 169
Eco81I CCTNAGG 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 36
EcoT38I GRGCYC 1 cut(s) 169
FaeI CATG 1 cut(s) 271
FaiI YATR 3 cut(s) 8, 249, 269
FalI AAGNNNNNCTT 2 cut(s) 54, 86
FatI CATG 1 cut(s) 267
Fnu4HI GCNGC 3 cut(s) 29, 109, 257
FokI GGATG 2 cut(s) 65, 168
FriOI GRGCYC 1 cut(s) 169
Fsp4HI GCNGC 3 cut(s) 29, 109, 257
GluI GCNGC 3 cut(s) 29, 109, 257
HaeIII GGCC 2 cut(s) 5, 207
HapII CCGG 1 cut(s) 76
Hin1II CATG 1 cut(s) 271
HinfI GANTC 1 cut(s) 274
HpaII CCGG 1 cut(s) 76
HphI GGTGA 2 cut(s) 65, 253
Hpy188III TCNNGA 1 cut(s) 278
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4V TGCA 4 cut(s) 22, 28, 195, 251
HpyF10VI GCNNNNNNNGC 1 cut(s) 28
HpyF3I CTNAG 1 cut(s) 140
Hsp92II CATG 1 cut(s) 271
Kzo9I GATC 1 cut(s) 88
LguI GCTCTTC 1 cut(s) 108
LpnPI CCDG 6 cut(s) 8, 23, 50, 89, 152, 224
Lsp1109I GCAGC 2 cut(s) 40, 268
MalI GATC 1 cut(s) 90
MboI GATC 1 cut(s) 88
MboII GAAGA 2 cut(s) 125, 283
MhlI GDGCHC 1 cut(s) 169
MlsI TGGCCA 2 cut(s) 5, 207
MluCI AATT 2 cut(s) 102, 286
MluNI TGGCCA 2 cut(s) 5, 207
MnlI CCTC 2 cut(s) 135, 156
Mox20I TGGCCA 2 cut(s) 5, 207
MscI TGGCCA 2 cut(s) 5, 207
Msp20I TGGCCA 2 cut(s) 5, 207
MspI CCGG 1 cut(s) 76
MspR9I CCNGG 1 cut(s) 38
MvaI CCWGG 1 cut(s) 38
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 1 cut(s) 28
NdeII GATC 1 cut(s) 88
NlaIII CATG 1 cut(s) 271
PaqCI CACCTGC 1 cut(s) 13
PciSI GCTCTTC 1 cut(s) 108
PfeI GAWTC 1 cut(s) 274
PinAI ACCGGT 1 cut(s) 75
PkrI GCNGC 3 cut(s) 30, 110, 258
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
SapI GCTCTTC 1 cut(s) 108
SatI GCNGC 3 cut(s) 29, 109, 257
Sau3AI GATC 1 cut(s) 88
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 1 cut(s) 169
SetI ASST 5 cut(s) 27, 120, 130, 240, 248
Sse9I AATT 2 cut(s) 102, 286
SsiI CCGC 1 cut(s) 109
StyD4I CCNGG 1 cut(s) 36
TaaI ACNGT 1 cut(s) 49
TasI AATT 2 cut(s) 102, 286
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 1 cut(s) 274
TscAI CASTG 1 cut(s) 54
TseI GCWGC 2 cut(s) 28, 256
TspDTI ATGAA 2 cut(s) 194, 284
TspRI CASTG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.