Rmu_sc0001407.1_g000014

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001407.1
OG0003455
Physical Location & Seq
Reverse (-)
88124 .. 88420
297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001407.1_g000014.1.cds

Sequence Viewer

Length: 297 bp
atggccataatatgttgtattcaggtgcagcctcaccaggagatgacagtggacgagttcaaagcgtggctgcgccggttcgacaatgatcacaatggacagatcagccgggaggagctaaaagaagctcttcacagcttgaggatttggttcgggtggtggagaagtcgtcaagtaatgaagcaagcagactccaataaagatggccaaatagacaacgccaaggaattcgaaaagctggtgaactatgctcagcaaaatttgcacatgaagatttatgagagtaatgggtggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5

Protein Analysis

98

Amino Acids

11.81

Weight (kDa)

6.4

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 2 cut(s) 3, 205
AcsI RAATTY 2 cut(s) 227, 259
AgsI TTSAA 1 cut(s) 61
AjnI CCWGG 1 cut(s) 36
AluBI AGCT 4 cut(s) 118, 128, 138, 238
AluI AGCT 4 cut(s) 118, 128, 138, 238
AoxI GGCC 2 cut(s) 3, 205
ApeKI GCWGC 2 cut(s) 28, 70
ApoI RAATTY 2 cut(s) 227, 259
Asp700I GAANNNNTTC 1 cut(s) 129
AspLEI GCGC 1 cut(s) 75
AsuC2I CCSGG 1 cut(s) 110
AsuHPI GGTGA 2 cut(s) 26, 253
AsuII TTCGAA 1 cut(s) 231
BalI TGGCCA 2 cut(s) 5, 207
BbvI GCAGC 2 cut(s) 40, 57
BccI CCATC 1 cut(s) 197
BciT130I CCWGG 1 cut(s) 38
BclI TGATCA 1 cut(s) 88
BcnI CCSGG 1 cut(s) 110
BisI GCNGC 2 cut(s) 29, 71
BlpI GCTNAGC 1 cut(s) 252
BlsI GCNGC 2 cut(s) 30, 72
Bme1390I CCNGG 2 cut(s) 38, 110
BmrFI CCNGG 2 cut(s) 38, 110
Bpu1102I GCTNAGC 1 cut(s) 252
Bpu14I TTCGAA 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 160
BpuMI CCSGG 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 222
BsaXI ACNNNNNCTCC 2 cut(s) 154, 184
Bse118I RCCGGY 1 cut(s) 75
BseBI CCWGG 1 cut(s) 38
BseDI CCNNGG 1 cut(s) 222
BseMII CTCAG 1 cut(s) 266
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 2 cut(s) 40, 57
BsgI GTGCAG 1 cut(s) 47
BshFI GGCC 2 cut(s) 5, 207
BsiSI CCGG 2 cut(s) 76, 109
BsnI GGCC 2 cut(s) 5, 207
Bsp119I TTCGAA 1 cut(s) 231
Bsp143I GATC 2 cut(s) 88, 102
Bsp1720I GCTNAGC 1 cut(s) 252
BspANI GGCC 2 cut(s) 5, 207
BspCNI CTCAG 1 cut(s) 265
BspQI GCTCTTC 1 cut(s) 135
BspT104I TTCGAA 1 cut(s) 231
BsrFI RCCGGY 1 cut(s) 75
BssAI RCCGGY 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 2 cut(s) 88, 102
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 1 cut(s) 49
Bst6I CTCTTC 1 cut(s) 135
BstAPI GCANNNNNTGC 1 cut(s) 262
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 1 cut(s) 252
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 2 cut(s) 91, 105
BstMBI GATC 2 cut(s) 88, 102
BstMWI GCNNNNNNNGC 1 cut(s) 262
BstNI CCWGG 1 cut(s) 38
BstSCI CCNGG 2 cut(s) 36, 108
BstV1I GCAGC 2 cut(s) 40, 57
BsuRI GGCC 2 cut(s) 5, 207
BtsIMutI CAGTG 1 cut(s) 54
Cac8I GCNNGC 1 cut(s) 186
CfoI GCGC 1 cut(s) 75
Cfr10I RCCGGY 1 cut(s) 75
CviAII CATG 1 cut(s) 268
CviJI RGCY 9 cut(s) 5, 31, 70, 108, 118, 128, 138, 207, 238
CviKI_1 RGCY 9 cut(s) 5, 31, 70, 108, 118, 128, 138, 207, 238
DdeI CTNAG 1 cut(s) 252
DpnI GATC 2 cut(s) 90, 104
DpnII GATC 2 cut(s) 88, 102
EaeI YGGCCR 2 cut(s) 3, 205
Eam1104I CTCTTC 1 cut(s) 135
EarI CTCTTC 1 cut(s) 135
Eco130I CCWWGG 1 cut(s) 222
EcoRI GAATTC 1 cut(s) 227
EcoRII CCWGG 1 cut(s) 36
EcoT14I CCWWGG 1 cut(s) 222
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 1 cut(s) 271
FaiI YATR 5 cut(s) 8, 13, 249, 269, 279
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FatI CATG 1 cut(s) 267
FbaI TGATCA 1 cut(s) 88
Fnu4HI GCNGC 2 cut(s) 29, 71
Fsp4HI GCNGC 2 cut(s) 29, 71
GlaI GCGC 1 cut(s) 74
GluI GCNGC 2 cut(s) 29, 71
HaeIII GGCC 2 cut(s) 5, 207
HapII CCGG 2 cut(s) 76, 109
HhaI GCGC 1 cut(s) 75
Hin1II CATG 1 cut(s) 271
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HinfI GANTC 1 cut(s) 191
HpaII CCGG 2 cut(s) 76, 109
HphI GGTGA 2 cut(s) 26, 253
Hpy166II GTNNAC 2 cut(s) 52, 244
Hpy8I GTNNAC 2 cut(s) 52, 244
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4V TGCA 2 cut(s) 28, 265
HpyF10VI GCNNNNNNNGC 1 cut(s) 262
HpyF3I CTNAG 1 cut(s) 252
Hsp92II CATG 1 cut(s) 271
HspAI GCGC 1 cut(s) 73
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 2 cut(s) 88, 102
LguI GCTCTTC 1 cut(s) 135
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 6 cut(s) 8, 23, 50, 89, 122, 224
Lsp1109I GCAGC 2 cut(s) 40, 57
MalI GATC 2 cut(s) 90, 104
MboI GATC 2 cut(s) 88, 102
MboII GAAGA 2 cut(s) 122, 283
MlsI TGGCCA 2 cut(s) 5, 207
MluCI AATT 2 cut(s) 227, 259
MluNI TGGCCA 2 cut(s) 5, 207
MlyI GAGTC 1 cut(s) 185
MnlI CCTC 3 cut(s) 42, 106, 135
Mox20I TGGCCA 2 cut(s) 5, 207
MroXI GAANNNNTTC 1 cut(s) 129
MscI TGGCCA 2 cut(s) 5, 207
Msp20I TGGCCA 2 cut(s) 5, 207
MspI CCGG 2 cut(s) 76, 109
MspR9I CCNGG 2 cut(s) 38, 110
MvaI CCWGG 1 cut(s) 38
MwoI GCNNNNNNNGC 1 cut(s) 262
NciI CCSGG 1 cut(s) 110
NdeII GATC 2 cut(s) 88, 102
NlaIII CATG 1 cut(s) 271
NspV TTCGAA 1 cut(s) 231
PciSI GCTCTTC 1 cut(s) 135
PdmI GAANNNNTTC 1 cut(s) 129
PkrI GCNGC 2 cut(s) 30, 72
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 36
SapI GCTCTTC 1 cut(s) 135
SatI GCNGC 2 cut(s) 29, 71
Sau3AI GATC 2 cut(s) 88, 102
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 2 cut(s) 38, 110
SetI ASST 5 cut(s) 27, 120, 130, 140, 240
SfuI TTCGAA 1 cut(s) 231
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
Sse9I AATT 2 cut(s) 227, 259
StyD4I CCNGG 2 cut(s) 36, 108
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 49
TaqI TCGA 2 cut(s) 81, 231
TasI AATT 2 cut(s) 227, 259
TscAI CASTG 1 cut(s) 54
TseI GCWGC 2 cut(s) 28, 70
TspDTI ATGAA 2 cut(s) 194, 284
TspRI CASTG 1 cut(s) 54
XapI RAATTY 2 cut(s) 227, 259
XmnI GAANNNNTTC 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.