FvH4_1g24010

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005653
Physical Location & Seq
Forward (+)
15879360 .. 15884905
5546 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g24010.t2

Sequence Viewer

Length: 2067 bp
ATGCTGATTGCTGATGAGGGTGAGAAGGATTTGTTCTTCGATTCCGCAGAGTACTTGTCATCTGAAGAGTCAGTGGTAGCAAAAGAGAACCTGGAGTGTAGCAAGTTGGAGTATGAGATTTGGGTGAATGAGCCACCAAGTGTGAAGGACAGACGAGAGAGTTTTCTTCGGCAGATGGGTTTAGCTGAATCAGGTTCTACAAAGTTTGGTTCTGTGGAGATGGAAAGGGACATCGGTACCTCATCAGAGATGATGGGATTGGAGAGACTTTCAGAGTGCAATGGAGCTGCCTCGAGCTCTTGCAGCTCCTCATCTAGTCAAATTGAGGAATACTTGGGATTTCATATCAGGGAAGGGGACAATCAAGCAAATTTTATGTGTGATGAATTTGTCAGAGGTCTACAAGACAAGTCAAATTTAGTTTACATTTTGGAGTCGGTACAAGTTTCAACATCTGGTAGTGATTGTTCTCAGAGGGAAACTTGTTCTGAGAGAGAAACTTCTTCTCACAGAGAAATTGAGGCTACTGAAGAAGAATGCGACAGTTCAAAGGAGGGCCACAAGAAGAAGAGAAGTTGGTGGAAACAGTTCATAAACAAGAGCAAGAGGAGAGGTGGTAAAGTTGTCTCAGAGGTATCAAAACTTGATACTGAAGTATCCAAAGAAAACAAAATGATAGTCAAACAGAACAGTAAGAGGTACATGGAGTTTGCCGCACTTTGCACCGGGCAAGAAATTCAGGCTCACAATGGGTTTATATGGACGATGAAGTTTAGTCCTGATGGTCAATACCTTGCAACTGGTGGTGAAGATGGGGTTGTACGCATTTGGCGTGTTACAGCAGATGCCTCTTGCAATTACCTAATGGCTCAAGGCAACTTAGATAGCAAACTTAAAAAGATCAAGTCTGGATGTCGAGTTATTTTTCCTGACAATAGTTTTCGAATTGAAGAGTCACCGCTCCAAGAGTTTCTTGGCCATACAAGTGATGTGTTAGACCTGGCTTGGTCAAATTCAAATTGTCTCATTTCTTCGTCCATGGATAAGACTGTTCGTCTGTGGGAAGTTGGTTGCAGTCGGTGCCTAAATGTTTTCCATCACAATGACTATGTGACATGCATTCAGTTCAATCCTCTTGATGACAGTTACTTCATCAGTGGATCCATCGATGGGAAGGTTCGAGTTTGGGGACTTTCTGAGAAGCGAGTTGTTGACTGGGCTGACGTGCGAGATGTCATAACAGCTATTTGTTATCAACCAGATGGAAAGGGTTTCGCAGTTGGTTCCCTTGCAGGCACTTGCCGCTTCTATGAAGAATCAGAAAAAGAAGCCCTTCAGCTTGTTGCACAGATTCATATTCGTTCTAGAAGGAAGACCTCTGGCAACAAAATCACCGGCATCCAGTTTTCCCAGGAAAACACTGGAAGAGTTATGATAACATCAGAAGACTCAAAAGTCCGTGTGTTTGATGGGGTAGATGTCATTAAGAAATACAGAGGTCTTCCAAAGTCAGGAAGTCAAATGTCAGCTTCTTTCACATCGAGTGGGAAACGTATTATCTCAGTTGGAGAGGACTCTCGTGTTTATTTATGGGACTATGACAGCTCTTGTCTTCCATCATCCAAGCAAACAAAATCTGTTCGATCATGTGAACATTTCTATTGCGAAGGTGTGTCTGTTGCAATCCCTTGGTCCGGCATGGGAACAGAGAAGATGAACTTCGAAGTTGCTAGTCCGCAATATTGCTCACAAACACAAGGCCGAATAGAGGCTACTCCTAGCAGTAGAGATTCGGAACGCTTTTTGCTTGGGAATTGGTTTTTCCTGGATGGACCCTGCAGAGGCTCTGCAACTTGGCCCGAGGAAAACCTTCCTCAGTGGGATTCACCGGTTGCTGGAGATGAAGATGATCACCAGCAACACGGAGAGCATCAACACCGTCACAACCACAGTAAAGCTCACAACCACTTGACGAAGTCAGAGACGTGGGGGCTTGTCATTGTAACTGCTGGATGGGATGGAAAGATCAGGACATTCCATAACTATGGATTGCCGGTCAGTTTGTAG

Protein Analysis

689

Amino Acids

77.37

Weight (kDa)

5.54

Isoelectric Point (pI)

54.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 231 - 280 3.7e-08 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 244 - 404 7.4e-17 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 245 - 277 1.5e-09 WD domain, G-beta repeat
Beta-prop_IFT122_1st PF23381 276 - 440 4.9e-06 IFT122 first beta-propeller
WD40_CDC20-Fz PF24807 314 - 419 1.5e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 317 - 397 8.8e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 318 - 549 1.3e-20 Prp19 WD40 domain
WD40 PF00400 319 - 354 3.7e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 320 - 364 2.1e-06 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 320 - 536 1.5e-34 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 322 - 381 2.2e-08 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 323 - 490 7.1e-10 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 327 - 548 3.1e-24 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 329 - 423 7.7e-06 Aladin seven-bladed propeller
Beta-prop_WDR3_2nd PF25172 330 - 534 9.2e-12 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 342 - 457 1e-08 WDR90/POC16, second beta-propeller
WD40_WDHD1_1st PF24817 371 - 545 2.5e-09 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 382 - 546 5.9e-14 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 401 - 535 9e-09 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1454
Acc65I GGTACC 1 cut(s) 236
AccB1I GGYRCC 2 cut(s) 236, 1080
AccBSI CCGCTC 1 cut(s) 961
AccI GTMKAC 1 cut(s) 400
AciI CCGC 5 cut(s) 45, 714, 959, 1303, 1736
AclWI GGATC 2 cut(s) 1155, 1168
AcoI YGGCCR 1 cut(s) 976
AcsI RAATTY 5 cut(s) 370, 386, 415, 735, 1012
AcuI CTGAAG 4 cut(s) 84, 549, 672, 1319
AdeI CACNNNGTG 1 cut(s) 140
AfaI GTAC 5 cut(s) 53, 238, 441, 701, 822
AfiI CCNNNNNNNGG 6 cut(s) 1170, 1511, 1694, 1768, 1841, 1895
AgeI ACCGGT 1 cut(s) 1888
AgsI TTSAA 5 cut(s) 450, 549, 950, 1017, 1129
AhdI GACNNNNNGTC 1 cut(s) 1053
AjiI CACGTC 2 cut(s) 1225, 1986
AjnI CCWGG 4 cut(s) 90, 999, 1410, 1824
AluBI AGCT 9 cut(s) 185, 287, 297, 306, 1244, 1339, 1529, 1605, 1958
AluI AGCT 9 cut(s) 185, 287, 297, 306, 1244, 1339, 1529, 1605, 1958
Alw21I GWGCWC 1 cut(s) 299
Alw26I GTCTC 4 cut(s) 259, 631, 1028, 1976
AlwI GGATC 2 cut(s) 1155, 1168
Ama87I CYCGRG 2 cut(s) 292, 1859
AoxI GGCC 4 cut(s) 556, 976, 1759, 1856
ApeKI GCWGC 2 cut(s) 287, 303
ApoI RAATTY 5 cut(s) 370, 386, 415, 735, 1012
ArsI GACNNNNNNTTYG 2 cut(s) 663, 695
AsiGI ACCGGT 1 cut(s) 1888
Asp700I GAANNNNTTC 3 cut(s) 587, 1332, 1869
Asp718I GGTACC 1 cut(s) 236
AspS9I GGNCC 4 cut(s) 556, 1692, 1832, 1857
AsuC2I CCSGG 1 cut(s) 727
AsuHPI GGTGA 7 cut(s) 32, 136, 818, 948, 1384, 1878, 1904
AsuII TTCGAA 2 cut(s) 943, 1722
AvaI CYCGRG 2 cut(s) 292, 1859
AvaII GGWCC 2 cut(s) 1692, 1832
BaeI ACNNNNGTAYC 6 cut(s) 220, 253, 639, 639, 672, 672
BalI TGGCCA 1 cut(s) 978
BamHI GGATCC 1 cut(s) 1160
BanI GGYRCC 2 cut(s) 236, 1080
BanII GRGCYC 1 cut(s) 299
BauI CACGAG 1 cut(s) 1578
BbsI GAAGAC 4 cut(s) 1379, 1452, 1493, 1604
Bbv12I GWGCWC 1 cut(s) 299
BbvI GCAGC 2 cut(s) 274, 315
BciT130I CCWGG 4 cut(s) 92, 1001, 1412, 1826
BciVI GTATCC 1 cut(s) 667
BclI TGATCA 1 cut(s) 1909
BcnI CCSGG 1 cut(s) 727
BcoDI GTCTC 4 cut(s) 259, 631, 1028, 1976
BfaI CTAG 4 cut(s) 315, 1365, 1731, 1779
BfmI CTRYAG 1 cut(s) 1837
BfuI GTATCC 1 cut(s) 667
BisI GCNGC 4 cut(s) 288, 304, 714, 1303
BlsI GCNGC 4 cut(s) 289, 305, 715, 1304
BmcAI AGTACT 1 cut(s) 53
Bme1390I CCNGG 5 cut(s) 92, 727, 1001, 1412, 1826
Bme18I GGWCC 2 cut(s) 1692, 1832
BmeRI GACNNNNNGTC 1 cut(s) 1053
BmeT110I CYCGRG 2 cut(s) 292, 1859
BmgBI CACGTC 2 cut(s) 1225, 1986
BmgT120I GGNCC 4 cut(s) 556, 1692, 1832, 1857
BmiI GGNNCC 5 cut(s) 238, 1082, 1162, 1285, 1834
BmrFI CCNGG 5 cut(s) 92, 727, 1001, 1412, 1826
BmrI ACTGGG 1 cut(s) 1225
BmsI GCATC 3 cut(s) 835, 1407, 1939
BmuI ACTGGG 1 cut(s) 1225
BpiI GAAGAC 4 cut(s) 1379, 1452, 1493, 1604
BpmI CTGGAG 2 cut(s) 113, 1917
Bpu14I TTCGAA 2 cut(s) 943, 1722
BpuEI CTTGAG 1 cut(s) 855
BpuMI CCSGG 1 cut(s) 727
Bsa29I ATCGAT 1 cut(s) 1167
BsaJI CCNNGG 4 cut(s) 1038, 1410, 1688, 1860
BsaWI WCCGGW 1 cut(s) 1888
BsaXI ACNNNNNCTCC 2 cut(s) 601, 631
Bsc4I CCNNNNNNNGG 6 cut(s) 1170, 1511, 1694, 1768, 1841, 1895
Bse118I RCCGGY 3 cut(s) 1394, 1888, 2053
Bse1I ACTGG 4 cut(s) 805, 1220, 1402, 1426
Bse3DI GCAATG 1 cut(s) 286
BseBI CCWGG 4 cut(s) 92, 1001, 1412, 1826
BseCI ATCGAT 1 cut(s) 1167
BseDI CCNNGG 4 cut(s) 1038, 1410, 1688, 1860
BseGI GGATG 6 cut(s) 917, 1398, 1619, 1834, 2018, 2023
BseLI CCNNNNNNNGG 6 cut(s) 1170, 1511, 1694, 1768, 1841, 1895
BseMI GCAATG 1 cut(s) 286
BseMII CTCAG 6 cut(s) 480, 485, 642, 1188, 1575, 1889
BseNI ACTGG 4 cut(s) 805, 1220, 1402, 1426
BseRI GAGGAG 2 cut(s) 298, 622
BseXI GCAGC 2 cut(s) 274, 315
BshFI GGCC 4 cut(s) 558, 978, 1761, 1858
BshNI GGYRCC 2 cut(s) 236, 1080
BshTI ACCGGT 1 cut(s) 1888
BshVI ATCGAT 1 cut(s) 1167
BsiHKAI GWGCWC 1 cut(s) 299
BsiHKCI CYCGRG 2 cut(s) 292, 1859
BsiSI CCGG 5 cut(s) 726, 1395, 1695, 1889, 2054
BslFI GGGAC 4 cut(s) 242, 371, 1203, 1607
BslI CCNNNNNNNGG 6 cut(s) 1170, 1511, 1694, 1768, 1841, 1895
BsmAI GTCTC 4 cut(s) 259, 631, 1028, 1976
BsmBI CGTCTC 1 cut(s) 1976
BsmFI GGGAC 4 cut(s) 242, 371, 1203, 1607
BsmI GAATGC 2 cut(s) 542, 1119
BsnI GGCC 4 cut(s) 558, 978, 1761, 1858
BsoBI CYCGRG 2 cut(s) 292, 1859
Bsp119I TTCGAA 2 cut(s) 943, 1722
Bsp1286I GDGCHC 1 cut(s) 299
Bsp143I GATC 5 cut(s) 900, 1160, 1643, 1909, 2025
Bsp19I CCATGG 1 cut(s) 1038
BspACI CCGC 5 cut(s) 45, 714, 959, 1303, 1736
BspANI GGCC 4 cut(s) 558, 978, 1761, 1858
BspCNI CTCAG 6 cut(s) 481, 484, 641, 1189, 1574, 1888
BspDI ATCGAT 1 cut(s) 1167
BspLI GGNNCC 5 cut(s) 238, 1082, 1162, 1285, 1834
BspMAI CTGCAG 1 cut(s) 1841
BspPI GGATC 2 cut(s) 1155, 1168
BspT104I TTCGAA 2 cut(s) 943, 1722
BspT107I GGYRCC 2 cut(s) 236, 1080
BsrBI CCGCTC 1 cut(s) 961
BsrDI GCAATG 1 cut(s) 286
BsrFI RCCGGY 3 cut(s) 1394, 1888, 2053
BsrI ACTGG 4 cut(s) 805, 1220, 1402, 1426
BssAI RCCGGY 3 cut(s) 1394, 1888, 2053
BssECI CCNNGG 4 cut(s) 1038, 1410, 1688, 1860
BssMI GATC 5 cut(s) 900, 1160, 1643, 1909, 2025
BssSI CACGAG 1 cut(s) 1578
BssT1I CCWWGG 2 cut(s) 1038, 1688
Bst2BI CACGAG 1 cut(s) 1578
Bst2UI CCWGG 4 cut(s) 92, 1001, 1412, 1826
Bst4CI ACNGT 7 cut(s) 545, 588, 692, 1051, 1145, 1940, 1952
Bst6I CTCTTC 4 cut(s) 60, 563, 945, 1420
BstAPI GCANNNNNTGC 1 cut(s) 1080
BstBI TTCGAA 2 cut(s) 943, 1722
BstC8I GCNNGC 1 cut(s) 1294
BstDEI CTNAG 7 cut(s) 471, 489, 628, 880, 1197, 1561, 1875
BstDSI CCRYGG 1 cut(s) 1038
BstF5I GGATG 6 cut(s) 917, 1398, 1619, 1834, 2018, 2023
BstKTI GATC 5 cut(s) 903, 1163, 1646, 1912, 2028
BstMAI GTCTC 4 cut(s) 259, 631, 1028, 1976
BstMBI GATC 5 cut(s) 900, 1160, 1643, 1909, 2025
BstMWI GCNNNNNNNGC 3 cut(s) 303, 1080, 1302
BstNI CCWGG 4 cut(s) 92, 1001, 1412, 1826
BstNSI RCATGY 1 cut(s) 1119
BstSCI CCNGG 5 cut(s) 90, 725, 999, 1410, 1824
BstSFI CTRYAG 1 cut(s) 1837
BstV1I GCAGC 2 cut(s) 274, 315
BstV2I GAAGAC 4 cut(s) 1379, 1452, 1493, 1604
BstX2I RGATCY 1 cut(s) 1160
BstXI CCANNNNNNTGG 1 cut(s) 2045
BstYI RGATCY 1 cut(s) 1160
Bsu15I ATCGAT 1 cut(s) 1167
BsuI GTATCC 1 cut(s) 667
BsuRI GGCC 4 cut(s) 558, 978, 1761, 1858
BsuTUI ATCGAT 1 cut(s) 1167
BtgI CCRYGG 1 cut(s) 1038
BtrI CACGTC 2 cut(s) 1225, 1986
BtsCI GGATG 6 cut(s) 917, 1398, 1619, 1834, 2018, 2023
BtsIMutI CAGTG 4 cut(s) 78, 1162, 1419, 1883
Cac8I GCNNGC 1 cut(s) 1294
Cfr10I RCCGGY 3 cut(s) 1394, 1888, 2053
Cfr13I GGNCC 4 cut(s) 556, 1692, 1832, 1857
ClaI ATCGAT 1 cut(s) 1167
Csp6I GTAC 5 cut(s) 52, 237, 440, 700, 821
CspAI ACCGGT 1 cut(s) 1888
CviAII CATG 5 cut(s) 703, 1039, 1116, 1647, 1699
CviQI GTAC 5 cut(s) 52, 237, 440, 700, 821
DdeI CTNAG 7 cut(s) 471, 489, 628, 880, 1197, 1561, 1875
DpnI GATC 5 cut(s) 902, 1162, 1645, 1911, 2027
DpnII GATC 5 cut(s) 900, 1160, 1643, 1909, 2025
DraIII CACNNNGTG 1 cut(s) 140
DrdI GACNNNNNNGTC 1 cut(s) 1454
DriI GACNNNNNGTC 1 cut(s) 1053
DseDI GACNNNNNNGTC 1 cut(s) 1454
EaeI YGGCCR 1 cut(s) 976
Eam1104I CTCTTC 4 cut(s) 60, 563, 945, 1420
Eam1105I GACNNNNNGTC 1 cut(s) 1053
EarI CTCTTC 4 cut(s) 60, 563, 945, 1420
Ecl136II GAGCTC 1 cut(s) 297
Eco130I CCWWGG 2 cut(s) 1038, 1688
Eco24I GRGCYC 1 cut(s) 299
Eco47I GGWCC 2 cut(s) 1692, 1832
Eco53kI GAGCTC 1 cut(s) 297
Eco57I CTGAAG 4 cut(s) 84, 549, 672, 1319
Eco88I CYCGRG 2 cut(s) 292, 1859
EcoICRI GAGCTC 1 cut(s) 297
EcoRII CCWGG 4 cut(s) 90, 999, 1410, 1824
EcoT14I CCWWGG 2 cut(s) 1038, 1688
EcoT22I ATGCAT 1 cut(s) 1121
EcoT38I GRGCYC 1 cut(s) 299
ErhI CCWWGG 2 cut(s) 1038, 1688
Esp3I CGTCTC 1 cut(s) 1976
FaeI CATG 5 cut(s) 706, 1042, 1119, 1650, 1702
FalI AAGNNNNNCTT 6 cut(s) 957, 989, 1317, 1349, 1703, 1735
FaqI GGGAC 4 cut(s) 242, 371, 1203, 1607
FatI CATG 5 cut(s) 702, 1038, 1115, 1646, 1698
FbaI TGATCA 1 cut(s) 1909
FblI GTMKAC 1 cut(s) 400
Fnu4HI GCNGC 4 cut(s) 288, 304, 714, 1303
FokI GGATG 6 cut(s) 924, 1385, 1606, 1841, 2025, 2030
FriOI GRGCYC 1 cut(s) 299
Fsp4HI GCNGC 4 cut(s) 288, 304, 714, 1303
FspBI CTAG 4 cut(s) 315, 1365, 1731, 1779
GluI GCNGC 4 cut(s) 288, 304, 714, 1303
GsuI CTGGAG 2 cut(s) 113, 1917
HaeIII GGCC 4 cut(s) 558, 978, 1761, 1858
HapII CCGG 5 cut(s) 726, 1395, 1695, 1889, 2054
Hin1II CATG 5 cut(s) 706, 1042, 1119, 1650, 1702
HincII GTYRAC 1 cut(s) 1213
HindII GTYRAC 1 cut(s) 1213
HpaII CCGG 5 cut(s) 726, 1395, 1695, 1889, 2054
HphI GGTGA 7 cut(s) 32, 136, 818, 948, 1384, 1878, 1904
Hpy166II GTNNAC 4 cut(s) 401, 424, 1213, 1652
Hpy188III TCNNGA 7 cut(s) 779, 909, 929, 1136, 1365, 1512, 2029
Hpy8I GTNNAC 4 cut(s) 401, 424, 1213, 1652
HpyAV CCTTC 8 cut(s) 19, 139, 347, 1168, 1343, 1362, 1661, 1880
HpyCH4III ACNGT 7 cut(s) 545, 588, 692, 1051, 1145, 1940, 1952
HpyCH4IV ACGT 3 cut(s) 1224, 1552, 1985
HpyF10VI GCNNNNNNNGC 3 cut(s) 303, 1080, 1302
HpyF3I CTNAG 7 cut(s) 471, 489, 628, 880, 1197, 1561, 1875
HpySE526I ACGT 3 cut(s) 1224, 1552, 1985
Hsp92II CATG 5 cut(s) 706, 1042, 1119, 1650, 1702
KpnI GGTACC 1 cut(s) 240
Ksp22I TGATCA 1 cut(s) 1909
Kzo9I GATC 5 cut(s) 900, 1160, 1643, 1909, 2025
LmnI GCTCC 3 cut(s) 284, 311, 966
Lsp1109I GCAGC 2 cut(s) 274, 315
LweI GCATC 3 cut(s) 835, 1407, 1939
MaeI CTAG 4 cut(s) 315, 1365, 1731, 1779
MaeII ACGT 3 cut(s) 1224, 1552, 1985
MaeIII GTNAC 6 cut(s) 835, 954, 1111, 1145, 1940, 2002
MalI GATC 5 cut(s) 902, 1162, 1645, 1911, 2027
MbiI CCGCTC 1 cut(s) 961
MboI GATC 5 cut(s) 900, 1160, 1643, 1909, 2025
MflI RGATCY 1 cut(s) 1160
MhlI GDGCHC 1 cut(s) 299
MlsI TGGCCA 1 cut(s) 978
MluNI TGGCCA 1 cut(s) 978
MlyI GAGTC 5 cut(s) 77, 443, 962, 1442, 1568
MmeI TCCRAC 2 cut(s) 87, 1546
Mox20I TGGCCA 1 cut(s) 978
Mph1103I ATGCAT 1 cut(s) 1121
MroXI GAANNNNTTC 3 cut(s) 587, 1332, 1869
MscI TGGCCA 1 cut(s) 978
MseI TTAA 2 cut(s) 894, 1485
MslI CAYNNNNRTG 3 cut(s) 984, 1101, 2043
Msp20I TGGCCA 1 cut(s) 978
MspI CCGG 5 cut(s) 726, 1395, 1695, 1889, 2054
MspR9I CCNGG 5 cut(s) 92, 727, 1001, 1412, 1826
Mva1269I GAATGC 2 cut(s) 542, 1119
MvaI CCWGG 4 cut(s) 92, 1001, 1412, 1826
MwoI GCNNNNNNNGC 3 cut(s) 303, 1080, 1302
NciI CCSGG 1 cut(s) 727
NcoI CCATGG 1 cut(s) 1038
NdeII GATC 5 cut(s) 900, 1160, 1643, 1909, 2025
NlaIII CATG 5 cut(s) 706, 1042, 1119, 1650, 1702
NlaIV GGNNCC 5 cut(s) 238, 1082, 1162, 1285, 1834
NmuCI GTSAC 3 cut(s) 954, 1111, 1940
NsiI ATGCAT 1 cut(s) 1121
NspI RCATGY 1 cut(s) 1119
NspV TTCGAA 2 cut(s) 943, 1722
PaeR7I CTCGAG 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 829
PctI GAATGC 2 cut(s) 542, 1119
PdmI GAANNNNTTC 3 cut(s) 587, 1332, 1869
PfeI GAWTC 6 cut(s) 41, 188, 1316, 1351, 1790, 1883
PflFI GACNNNGTC 1 cut(s) 1975
PfoI TCCNGGA 1 cut(s) 1824
PinAI ACCGGT 1 cut(s) 1888
PkrI GCNGC 4 cut(s) 289, 305, 715, 1304
PleI GAGTC 5 cut(s) 76, 442, 961, 1442, 1568
PpsI GAGTC 5 cut(s) 76, 442, 961, 1442, 1568
Psp124BI GAGCTC 1 cut(s) 299
Psp6I CCWGG 4 cut(s) 90, 999, 1410, 1824
PspGI CCWGG 4 cut(s) 90, 999, 1410, 1824
PspN4I GGNNCC 5 cut(s) 238, 1082, 1162, 1285, 1834
PspPI GGNCC 4 cut(s) 556, 1692, 1832, 1857
PspXI VCTCGAGB 1 cut(s) 292
PsrI GAACNNNNNNTAC 2 cut(s) 451, 483
PstI CTGCAG 1 cut(s) 1841
PsuI RGATCY 1 cut(s) 1160
PsyI GACNNNGTC 1 cut(s) 1975
RsaI GTAC 5 cut(s) 53, 238, 441, 701, 822
RsaNI GTAC 5 cut(s) 52, 237, 440, 700, 821
RseI CAYNNNNRTG 3 cut(s) 984, 1101, 2043
SacI GAGCTC 1 cut(s) 299
SaqAI TTAA 2 cut(s) 894, 1485
SatI GCNGC 4 cut(s) 288, 304, 714, 1303
Sau3AI GATC 5 cut(s) 900, 1160, 1643, 1909, 2025
Sau96I GGNCC 4 cut(s) 556, 1692, 1832, 1857
ScaI AGTACT 1 cut(s) 53
SchI GAGTC 5 cut(s) 77, 443, 962, 1442, 1568
ScrFI CCNGG 5 cut(s) 92, 727, 1001, 1412, 1826
SduI GDGCHC 1 cut(s) 299
SfaNI GCATC 3 cut(s) 835, 1407, 1939
SfcI CTRYAG 1 cut(s) 1837
Sfr274I CTCGAG 1 cut(s) 292
SfuI TTCGAA 2 cut(s) 943, 1722
SinI GGWCC 2 cut(s) 1692, 1832
SlaI CTCGAG 1 cut(s) 292
SmiMI CAYNNNNRTG 3 cut(s) 984, 1101, 2043
SmlI CTYRAG 2 cut(s) 292, 870
SmoI CTYRAG 2 cut(s) 292, 870
SsiI CCGC 5 cut(s) 45, 714, 959, 1303, 1736
SspI AATATT 1 cut(s) 1742
SspMI CTAG 4 cut(s) 315, 1365, 1731, 1779
SstI GAGCTC 1 cut(s) 299
StyD4I CCNGG 5 cut(s) 90, 725, 999, 1410, 1824
StyI CCWWGG 2 cut(s) 1038, 1688
TaaI ACNGT 7 cut(s) 545, 588, 692, 1051, 1145, 1940, 1952
TaiI ACGT 3 cut(s) 1227, 1555, 1988
TaqI TCGA 9 cut(s) 39, 293, 916, 943, 1167, 1180, 1541, 1642, 1722
TatI WGTACW 1 cut(s) 51
TauI GCSGC 2 cut(s) 716, 1305
TfiI GAWTC 6 cut(s) 41, 188, 1316, 1351, 1790, 1883
Tru1I TTAA 2 cut(s) 894, 1485
Tru9I TTAA 2 cut(s) 894, 1485
TscAI CASTG 4 cut(s) 78, 1162, 1426, 1883
TseFI GTSAC 3 cut(s) 954, 1111, 1940
TseI GCWGC 2 cut(s) 287, 303
Tsp45I GTSAC 3 cut(s) 954, 1111, 1940
TspDTI ATGAA 9 cut(s) 332, 399, 580, 782, 1141, 1326, 1343, 1730, 1917
TspGWI ACGGA 2 cut(s) 1448, 1938
TspRI CASTG 4 cut(s) 78, 1162, 1426, 1883
Tth111I GACNNNGTC 1 cut(s) 1975
VpaK11BI GGWCC 2 cut(s) 1692, 1832
XapI RAATTY 5 cut(s) 370, 386, 415, 735, 1012
XbaI TCTAGA 1 cut(s) 1364
XceI RCATGY 1 cut(s) 1119
XcmI CCANNNNNNNNNTGG 2 cut(s) 971, 1418
XhoI CTCGAG 1 cut(s) 292
XmiI GTMKAC 1 cut(s) 400
XmnI GAANNNNTTC 3 cut(s) 587, 1332, 1869
XspI CTAG 4 cut(s) 315, 1365, 1731, 1779
ZrmI AGTACT 1 cut(s) 53
Zsp2I ATGCAT 1 cut(s) 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.