Rw2G022730

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005653
Physical Location & Seq
Forward (+)
32600923 .. 32604412
3490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G022730.1

Sequence Viewer

Length: 2016 bp
ATGCTGAGTTCCGATGGGGGTGAGAAGGATTTGTTCTTTGATTCCGCAGAGTATTTGTCGTCTGAAGAGTCAGTGGTAGTGAAGGAGGAGTTGGAGTATGAGATTTGGTTGAATGAGCCACCGAGTGTGAAGGAAAGACGAGAGAGTTTTCTACGGCAGATGGGTTTAGCTGAATCTGGATCTACAAAGTTTGGTTCTGTGGAGATGGAAAGGGACGTTGGTACCTCATCAGAGATGATGGGATTGGAGAGACTTTTGGAGTGGAGTGGAGCTGCCTCGAGCTCTTGCAGCTCCTCCTCTAGTCACATTGAGGAATATTCGGGTTTTCATAGCAGGGAAGGGGACAATCTAGCAAATTTTATGTATGATGAATTGGACAGAGACCTGCAAGACAAGTCAAATTTAGTTTCCATTCTAGAGTCAGTACAAGTTTCGACTTCTGGTAGTGATTGTTCTCAGGGGGAAACTTGTTCTGAGAGAGAAACCGAGGCTACTGAAGAAGAATGCGAGAGTTCAAAGGAGGGCCTGAAGAAGAAGAGAAGTTGGTGGAAACAGTTTATAAACAAGAGCAAGAGGAGAGGAGGTAAAGTTGTCTCAGAGGTATCAAAACCTGATACTGAAGTATCCAAAGAAAACAGAATGAAGATCAAACAGAACAGTAAGAGGTACATGGAGTTTTCAGCACTTTGCACCGGGCAAGAAATTCAGGCTCACAATGGGTTTATTTGGACGATGAAGTTTAGTCCTGATGGCCAATACATTGCAAGTGGTGGTGAAGATGGGGTTGTGCGCATTTGGTGTGTTACACCAGATGCCTCTTGTAATTACCTTATGGCTCAAGGCAACTTAGATAGCAAACTTAAAAAGATCAAGTCTGGATGTCGAGTTATTTTTCCTGACAACAGTTTTCGAATTGAAGAGTCACCTCTCCAAGAGTTTCTTGGCCATTCCAGTGATGTGTTAGACCTGGCTTGGTCAAATTCAAACCGTCTCATTTCTTCGTCCATGGATAAAACTGTTCGTCTGTGGGAAGTTGGTTGCAGTCAGTGCCTACATGTTTTCCATCACAATGACTATGTAACATGCATTCAGTTCAATCCTCTCAATCACAATTACTTCATCAGTGGATCCATAGATGGAAAGGCTCGAGTTTGGGGGCTTTCTGAGAAGCGAGTTGTTGACTGGGCTGATGTGCGAGATGTCATAACAGCTATTTGTTATCAACCAGATGGGAAGGGTTTCGCAGTTGGTTCCCTTGCAGGCACTTGCCGCTTCTATGAAGAATCAGGAAAAGAAGCTCTTCAGCTTGTTGCACAGATTCATATTCATTCTAGAAGGAAGACCTCTGGCAACAAAATCACCGAAAATGCTCAAAGAGTTATGATAACATCAGAAGACTCAAAAGTCCGTGTGTTTGATGGGGTAGATCTCATTAAGAAATACAGAGGTCTTCCAAAGTCAGGAAGTCAAATGTCAGCTTCTTTCACATCCAGCGGGAAACGTATAATCTCAGTTGGAGAGGACTCGCGCGTTTATTTATGGGACTATGACAGCTGTAGTGTTCCATCATCCAAGCATACAAAATCTGTTCGATCCTGCGAACATTTCTATTGCGAAGGTGTGTCTGTTGCAATCCCTTGGTCAGGCATGGGAACAGAAGATAGGAACTTAGAAACTGCTAGTCCACGATATTGCTCACAAACACAAGGCCGAATAGACGCTACTCCTGGCAGTAGAGATTCGGAACGCTTTTTGCTTGGGAATTGGTTCTTTCTGGAGGGTCCCTGCAGAGGCTCTGCAACTTGGCCTGAGGAAAACCTTCCTCAGTGGGATTCACCAGTTGCAGGAGAGGAAGATGATCAGTGGCAATACAAAGACCATCAACACCATCACCACCACAATAAAGCTCACAACCACTTGACGAAATCAGAAACGTGGGGGCTTGTCATTGTAACTGCGGGATGGGATGGAAAAATCAGGACATTCCATAACTATGGATTGCCGGTCAGCTTGTAG

Protein Analysis

671

Amino Acids

75.53

Weight (kDa)

5.59

Isoelectric Point (pI)

50.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 222 - 268 3.1e-07 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 233 - 370 7.1e-13 CAF1B/HIR1 beta-propeller domain
WD40 PF00400 234 - 266 1.9e-09 WD domain, G-beta repeat
WD40_Gbeta PF25391 240 - 385 5.7e-06 G protein beta WD-40 repeat protein
Beta-prop_IFT122_1st PF23381 292 - 435 2.9e-08 IFT122 first beta-propeller
WD40_CDC20-Fz PF24807 303 - 393 9.6e-09 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 306 - 386 5.3e-11 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 307 - 532 6.7e-20 Prp19 WD40 domain
WD40 PF00400 308 - 343 2.4e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 309 - 355 7.6e-07 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 309 - 523 7.5e-35 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 311 - 369 3.1e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd PF25172 312 - 428 3.2e-08 WDR3 second beta-propeller domain
WDR55 PF24796 312 - 473 1.8e-06 WDR55
Beta-prop_THOC3 PF25174 316 - 544 7.2e-24 THOC3 beta-propeller domain
WD40_Gbeta PF25391 323 - 432 2.7e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd PF23393 328 - 440 6.9e-09 WDR90/POC16, second beta-propeller
Beta-prop_EML_2 PF23414 366 - 531 8.3e-13 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 390 - 531 5.5e-09 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 560
AasI GACNNNNNNGTC 1 cut(s) 1403
Acc16I TGCGCA 1 cut(s) 791
Acc36I ACCTGC 1 cut(s) 393
Acc65I GGTACC 1 cut(s) 221
AccB1I GGYRCC 1 cut(s) 221
AccII CGCG 2 cut(s) 1528, 1530
AciI CCGC 4 cut(s) 45, 1270, 1494, 1958
AclWI GGATC 4 cut(s) 187, 1122, 1135, 1587
AcoI YGGCCR 2 cut(s) 751, 943
AcsI RAATTY 4 cut(s) 355, 400, 702, 979
AcuI CTGAAG 5 cut(s) 84, 516, 548, 639, 1286
AdeI CACNNNGTG 1 cut(s) 125
AfaI GTAC 3 cut(s) 223, 426, 668
AfiI CCNNNNNNNGG 4 cut(s) 1460, 1643, 1790, 1844
AflIII ACRYGT 1 cut(s) 1054
AgsI TTSAA 5 cut(s) 112, 516, 917, 984, 1096
AjnI CCWGG 2 cut(s) 966, 1726
AjuI GAANNNNNNNTTGG 4 cut(s) 74, 106, 201, 233
Alw21I GWGCWC 1 cut(s) 284
Alw26I GTCTC 4 cut(s) 244, 375, 598, 995
AlwI GGATC 4 cut(s) 187, 1122, 1135, 1587
Ama87I CYCGRG 2 cut(s) 277, 1146
AoxI GGCC 5 cut(s) 523, 751, 943, 1708, 1805
ApeKI GCWGC 2 cut(s) 272, 288
ApoI RAATTY 4 cut(s) 355, 400, 702, 979
Asp700I GAANNNNTTC 4 cut(s) 1238, 1299, 1766, 1818
Asp718I GGTACC 1 cut(s) 221
AspLEI GCGC 2 cut(s) 792, 1530
AspS9I GGNCC 2 cut(s) 523, 1781
AsuC2I CCSGG 1 cut(s) 694
AsuHPI GGTGA 6 cut(s) 32, 785, 915, 1351, 1827, 1883
AsuII TTCGAA 1 cut(s) 910
AvaI CYCGRG 2 cut(s) 277, 1146
AvaII GGWCC 1 cut(s) 1781
AxyI CCTNAGG 1 cut(s) 1809
BaeI ACNNNNGTAYC 6 cut(s) 205, 238, 606, 606, 639, 639
BalI TGGCCA 2 cut(s) 753, 945
BamHI GGATCC 1 cut(s) 1127
BanI GGYRCC 1 cut(s) 221
BanII GRGCYC 1 cut(s) 284
BbsI GAAGAC 3 cut(s) 1346, 1401, 1442
Bbv12I GWGCWC 1 cut(s) 284
BbvI GCAGC 2 cut(s) 259, 300
BceAI ACGGC 1 cut(s) 170
BciT130I CCWGG 2 cut(s) 968, 1728
BciVI GTATCC 1 cut(s) 634
BclI TGATCA 1 cut(s) 1858
BcnI CCSGG 1 cut(s) 694
BcoDI GTCTC 4 cut(s) 244, 375, 598, 995
BfaI CTAG 5 cut(s) 300, 350, 416, 1332, 1680
BfmI CTRYAG 2 cut(s) 1555, 1786
BfuAI ACCTGC 1 cut(s) 393
BfuI GTATCC 1 cut(s) 634
BglII AGATCT 1 cut(s) 1426
BisI GCNGC 3 cut(s) 273, 289, 1270
BlsI GCNGC 3 cut(s) 274, 290, 1271
Bme1390I CCNGG 3 cut(s) 694, 968, 1728
Bme18I GGWCC 1 cut(s) 1781
BmeT110I CYCGRG 2 cut(s) 277, 1146
BmgT120I GGNCC 2 cut(s) 523, 1781
BmiI GGNNCC 5 cut(s) 223, 1129, 1252, 1782, 1783
BmrFI CCNGG 3 cut(s) 694, 968, 1728
BmrI ACTGGG 1 cut(s) 1192
BmsI GCATC 1 cut(s) 802
BmuI ACTGGG 1 cut(s) 1192
BpiI GAAGAC 3 cut(s) 1346, 1401, 1442
BpmI CTGGAG 1 cut(s) 1796
Bpu14I TTCGAA 1 cut(s) 910
BpuEI CTTGAG 1 cut(s) 822
BpuMI CCSGG 1 cut(s) 694
BsaI GGTCTC 1 cut(s) 375
BsaJI CCNNGG 3 cut(s) 486, 1005, 1637
BsaXI ACNNNNNCTCC 6 cut(s) 80, 110, 568, 573, 598, 603
Bsc4I CCNNNNNNNGG 4 cut(s) 1460, 1643, 1790, 1844
Bse118I RCCGGY 1 cut(s) 2002
Bse1I ACTGG 3 cut(s) 951, 1187, 1838
Bse21I CCTNAGG 1 cut(s) 1809
Bse3DI GCAATG 1 cut(s) 759
BseBI CCWGG 2 cut(s) 968, 1728
BseDI CCNNGG 3 cut(s) 486, 1005, 1637
BseGI GGATG 5 cut(s) 884, 1487, 1568, 1967, 1972
BseLI CCNNNNNNNGG 4 cut(s) 1460, 1643, 1790, 1844
BseMI GCAATG 1 cut(s) 759
BseMII CTCAG 7 cut(s) 465, 470, 609, 1155, 1524, 1800, 1838
BseNI ACTGG 3 cut(s) 951, 1187, 1838
BseRI GAGGAG 5 cut(s) 101, 283, 286, 589, 594
BseXI GCAGC 2 cut(s) 259, 300
Bsh1236I CGCG 2 cut(s) 1528, 1530
BshFI GGCC 5 cut(s) 525, 753, 945, 1710, 1807
BshNI GGYRCC 1 cut(s) 221
BsiHKAI GWGCWC 1 cut(s) 284
BsiHKCI CYCGRG 2 cut(s) 277, 1146
BsiSI CCGG 2 cut(s) 693, 2003
BslFI GGGAC 4 cut(s) 227, 356, 1556, 1767
BslI CCNNNNNNNGG 4 cut(s) 1460, 1643, 1790, 1844
BsmAI GTCTC 4 cut(s) 244, 375, 598, 995
BsmBI CGTCTC 1 cut(s) 995
BsmFI GGGAC 4 cut(s) 227, 356, 1556, 1767
BsmI GAATGC 2 cut(s) 509, 1086
BsnI GGCC 5 cut(s) 525, 753, 945, 1710, 1807
Bso31I GGTCTC 1 cut(s) 375
BsoBI CYCGRG 2 cut(s) 277, 1146
Bsp119I TTCGAA 1 cut(s) 910
Bsp1286I GDGCHC 1 cut(s) 284
Bsp143I GATC 7 cut(s) 179, 645, 867, 1127, 1426, 1592, 1858
Bsp19I CCATGG 1 cut(s) 1005
BspACI CCGC 4 cut(s) 45, 1270, 1494, 1958
BspANI GGCC 5 cut(s) 525, 753, 945, 1710, 1807
BspCNI CTCAG 7 cut(s) 466, 469, 608, 1156, 1523, 1801, 1837
BspFNI CGCG 2 cut(s) 1528, 1530
BspLI GGNNCC 5 cut(s) 223, 1129, 1252, 1782, 1783
BspMAI CTGCAG 1 cut(s) 1790
BspMI ACCTGC 1 cut(s) 393
BspPI GGATC 4 cut(s) 187, 1122, 1135, 1587
BspQI GCTCTTC 1 cut(s) 1305
BspT104I TTCGAA 1 cut(s) 910
BspT107I GGYRCC 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 375
BsrDI GCAATG 1 cut(s) 759
BsrFI RCCGGY 1 cut(s) 2002
BsrI ACTGG 3 cut(s) 951, 1187, 1838
BssAI RCCGGY 1 cut(s) 2002
BssECI CCNNGG 3 cut(s) 486, 1005, 1637
BssMI GATC 7 cut(s) 179, 645, 867, 1127, 1426, 1592, 1858
BssT1I CCWWGG 2 cut(s) 1005, 1637
Bst2UI CCWGG 2 cut(s) 968, 1728
Bst4CI ACNGT 5 cut(s) 555, 659, 905, 989, 1018
Bst6I CTCTTC 4 cut(s) 60, 530, 912, 1305
BstAPI GCANNNNNTGC 1 cut(s) 1047
BstBI TTCGAA 1 cut(s) 910
BstC8I GCNNGC 1 cut(s) 1261
BstDSI CCRYGG 1 cut(s) 1005
BstENI CCTNNNNNAGG 1 cut(s) 1641
BstF5I GGATG 5 cut(s) 884, 1487, 1568, 1967, 1972
BstFNI CGCG 2 cut(s) 1528, 1530
BstHHI GCGC 2 cut(s) 792, 1530
BstKTI GATC 7 cut(s) 182, 648, 870, 1130, 1429, 1595, 1861
BstMAI GTCTC 4 cut(s) 244, 375, 598, 995
BstMBI GATC 7 cut(s) 179, 645, 867, 1127, 1426, 1592, 1858
BstMWI GCNNNNNNNGC 3 cut(s) 288, 1047, 1269
BstNI CCWGG 2 cut(s) 968, 1728
BstNSI RCATGY 2 cut(s) 1058, 1086
BstSCI CCNGG 3 cut(s) 692, 966, 1726
BstSFI CTRYAG 2 cut(s) 1555, 1786
BstUI CGCG 2 cut(s) 1528, 1530
BstV1I GCAGC 2 cut(s) 259, 300
BstV2I GAAGAC 3 cut(s) 1346, 1401, 1442
BstX2I RGATCY 3 cut(s) 179, 1127, 1426
BstXI CCANNNNNNTGG 1 cut(s) 1994
BstYI RGATCY 3 cut(s) 179, 1127, 1426
Bsu36I CCTNAGG 1 cut(s) 1809
BsuI GTATCC 1 cut(s) 634
BsuRI GGCC 5 cut(s) 525, 753, 945, 1710, 1807
BtgI CCRYGG 1 cut(s) 1005
BtsCI GGATG 5 cut(s) 884, 1487, 1568, 1967, 1972
BtsIMutI CAGTG 6 cut(s) 78, 958, 1052, 1129, 1832, 1868
BveI ACCTGC 1 cut(s) 393
Cac8I GCNNGC 1 cut(s) 1261
CfoI GCGC 2 cut(s) 792, 1530
Cfr10I RCCGGY 1 cut(s) 2002
Cfr13I GGNCC 2 cut(s) 523, 1781
CseI GACGC 1 cut(s) 1727
Csp6I GTAC 3 cut(s) 222, 425, 667
CviAII CATG 5 cut(s) 670, 1006, 1055, 1083, 1648
CviQI GTAC 3 cut(s) 222, 425, 667
DpnI GATC 7 cut(s) 181, 647, 869, 1129, 1428, 1594, 1860
DpnII GATC 7 cut(s) 179, 645, 867, 1127, 1426, 1592, 1858
DraIII CACNNNGTG 1 cut(s) 125
DrdI GACNNNNNNGTC 1 cut(s) 1403
DseDI GACNNNNNNGTC 1 cut(s) 1403
EaeI YGGCCR 2 cut(s) 751, 943
Eam1104I CTCTTC 4 cut(s) 60, 530, 912, 1305
EarI CTCTTC 4 cut(s) 60, 530, 912, 1305
Ecl136II GAGCTC 1 cut(s) 282
Eco130I CCWWGG 2 cut(s) 1005, 1637
Eco24I GRGCYC 1 cut(s) 284
Eco31I GGTCTC 1 cut(s) 375
Eco47I GGWCC 1 cut(s) 1781
Eco53kI GAGCTC 1 cut(s) 282
Eco57I CTGAAG 5 cut(s) 84, 516, 548, 639, 1286
Eco81I CCTNAGG 1 cut(s) 1809
Eco88I CYCGRG 2 cut(s) 277, 1146
EcoICRI GAGCTC 1 cut(s) 282
EcoNI CCTNNNNNAGG 1 cut(s) 1641
EcoO109I RGGNCCY 2 cut(s) 523, 1781
EcoRII CCWGG 2 cut(s) 966, 1726
EcoT14I CCWWGG 2 cut(s) 1005, 1637
EcoT22I ATGCAT 1 cut(s) 1088
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 2 cut(s) 1005, 1637
Esp3I CGTCTC 1 cut(s) 995
FaeI CATG 5 cut(s) 673, 1009, 1058, 1086, 1651
FalI AAGNNNNNCTT 4 cut(s) 924, 956, 1284, 1316
FaqI GGGAC 4 cut(s) 227, 356, 1556, 1767
FatI CATG 5 cut(s) 669, 1005, 1054, 1082, 1647
FauI CCCGC 2 cut(s) 1487, 1951
FbaI TGATCA 1 cut(s) 1858
Fnu4HI GCNGC 3 cut(s) 273, 289, 1270
FokI GGATG 5 cut(s) 891, 1474, 1555, 1974, 1979
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 3 cut(s) 273, 289, 1270
FspAI RTGCGCAY 1 cut(s) 791
FspBI CTAG 5 cut(s) 300, 350, 416, 1332, 1680
FspI TGCGCA 1 cut(s) 791
GlaI GCGC 2 cut(s) 791, 1529
GluI GCNGC 3 cut(s) 273, 289, 1270
GsuI CTGGAG 1 cut(s) 1796
HaeIII GGCC 5 cut(s) 525, 753, 945, 1710, 1807
HapII CCGG 2 cut(s) 693, 2003
HgaI GACGC 1 cut(s) 1727
HhaI GCGC 2 cut(s) 792, 1530
Hin1II CATG 5 cut(s) 673, 1009, 1058, 1086, 1651
Hin6I GCGC 2 cut(s) 790, 1528
HinP1I GCGC 2 cut(s) 790, 1528
HincII GTYRAC 1 cut(s) 1180
HindII GTYRAC 1 cut(s) 1180
HpaII CCGG 2 cut(s) 693, 2003
HphI GGTGA 6 cut(s) 32, 785, 915, 1351, 1827, 1883
Hpy166II GTNNAC 2 cut(s) 1180, 1685
Hpy188I TCNGA 9 cut(s) 13, 64, 232, 475, 598, 1165, 1393, 1744, 1930
Hpy8I GTNNAC 2 cut(s) 1180, 1685
HpyAV CCTTC 8 cut(s) 19, 76, 124, 332, 1228, 1329, 1610, 1829
HpyCH4III ACNGT 5 cut(s) 555, 659, 905, 989, 1018
HpyCH4IV ACGT 3 cut(s) 216, 1501, 1934
HpyF10VI GCNNNNNNNGC 3 cut(s) 288, 1047, 1269
HpySE526I ACGT 3 cut(s) 216, 1501, 1934
Hsp92II CATG 5 cut(s) 673, 1009, 1058, 1086, 1651
HspAI GCGC 2 cut(s) 790, 1528
KflI GGGWCCC 1 cut(s) 1781
KpnI GGTACC 1 cut(s) 225
Ksp22I TGATCA 1 cut(s) 1858
Kzo9I GATC 7 cut(s) 179, 645, 867, 1127, 1426, 1592, 1858
LguI GCTCTTC 1 cut(s) 1305
LmnI GCTCC 2 cut(s) 269, 296
Lsp1109I GCAGC 2 cut(s) 259, 300
LweI GCATC 1 cut(s) 802
MaeI CTAG 5 cut(s) 300, 350, 416, 1332, 1680
MaeII ACGT 3 cut(s) 216, 1501, 1934
MaeIII GTNAC 5 cut(s) 302, 802, 921, 1078, 1951
MalI GATC 7 cut(s) 181, 647, 869, 1129, 1428, 1594, 1860
MboI GATC 7 cut(s) 179, 645, 867, 1127, 1426, 1592, 1858
MflI RGATCY 3 cut(s) 179, 1127, 1426
MhlI GDGCHC 1 cut(s) 284
MlsI TGGCCA 2 cut(s) 753, 945
MluCI AATT 9 cut(s) 355, 371, 400, 702, 823, 912, 979, 1111, 1762
MluNI TGGCCA 2 cut(s) 753, 945
MlyI GAGTC 5 cut(s) 77, 428, 929, 1391, 1517
MmeI TCCRAC 2 cut(s) 72, 1495
Mox20I TGGCCA 2 cut(s) 753, 945
Mph1103I ATGCAT 1 cut(s) 1088
MroXI GAANNNNTTC 4 cut(s) 1238, 1299, 1766, 1818
MscI TGGCCA 2 cut(s) 753, 945
MseI TTAA 2 cut(s) 861, 1434
MslI CAYNNNNRTG 3 cut(s) 951, 1068, 1992
Msp20I TGGCCA 2 cut(s) 753, 945
MspA1I CMGCKG 2 cut(s) 1494, 1554
MspI CCGG 2 cut(s) 693, 2003
MspR9I CCNGG 3 cut(s) 694, 968, 1728
Mva1269I GAATGC 2 cut(s) 509, 1086
MvaI CCWGG 2 cut(s) 968, 1728
MvnI CGCG 2 cut(s) 1528, 1530
MwoI GCNNNNNNNGC 3 cut(s) 288, 1047, 1269
NciI CCSGG 1 cut(s) 694
NcoI CCATGG 1 cut(s) 1005
NdeII GATC 7 cut(s) 179, 645, 867, 1127, 1426, 1592, 1858
NlaIII CATG 5 cut(s) 673, 1009, 1058, 1086, 1651
NlaIV GGNNCC 5 cut(s) 223, 1129, 1252, 1782, 1783
NmuCI GTSAC 2 cut(s) 302, 921
NsbI TGCGCA 1 cut(s) 791
NsiI ATGCAT 1 cut(s) 1088
NspI RCATGY 2 cut(s) 1058, 1086
NspV TTCGAA 1 cut(s) 910
PaeR7I CTCGAG 2 cut(s) 277, 1146
PciI ACATGT 1 cut(s) 1054
PciSI GCTCTTC 1 cut(s) 1305
PctI GAATGC 2 cut(s) 509, 1086
PdmI GAANNNNTTC 4 cut(s) 1238, 1299, 1766, 1818
PfeI GAWTC 6 cut(s) 41, 173, 1283, 1318, 1739, 1832
PkrI GCNGC 3 cut(s) 274, 290, 1271
PleI GAGTC 5 cut(s) 76, 427, 928, 1391, 1517
PpsI GAGTC 5 cut(s) 76, 427, 928, 1391, 1517
PpuMI RGGWCCY 1 cut(s) 1781
PscI ACATGT 1 cut(s) 1054
PsiI TTATAA 1 cut(s) 560
Psp124BI GAGCTC 1 cut(s) 284
Psp5II RGGWCCY 1 cut(s) 1781
Psp6I CCWGG 2 cut(s) 966, 1726
PspGI CCWGG 2 cut(s) 966, 1726
PspN4I GGNNCC 5 cut(s) 223, 1129, 1252, 1782, 1783
PspPI GGNCC 2 cut(s) 523, 1781
PspPPI RGGWCCY 1 cut(s) 1781
PspXI VCTCGAGB 2 cut(s) 277, 1146
PsrI GAACNNNNNNTAC 2 cut(s) 436, 468
PstI CTGCAG 1 cut(s) 1790
PsuI RGATCY 3 cut(s) 179, 1127, 1426
PvuII CAGCTG 1 cut(s) 1554
RsaI GTAC 3 cut(s) 223, 426, 668
RsaNI GTAC 3 cut(s) 222, 425, 667
RseI CAYNNNNRTG 3 cut(s) 951, 1068, 1992
SacI GAGCTC 1 cut(s) 284
SapI GCTCTTC 1 cut(s) 1305
SaqAI TTAA 2 cut(s) 861, 1434
SatI GCNGC 3 cut(s) 273, 289, 1270
Sau3AI GATC 7 cut(s) 179, 645, 867, 1127, 1426, 1592, 1858
Sau96I GGNCC 2 cut(s) 523, 1781
SchI GAGTC 5 cut(s) 77, 428, 929, 1391, 1517
ScrFI CCNGG 3 cut(s) 694, 968, 1728
SduI GDGCHC 1 cut(s) 284
SfaNI GCATC 1 cut(s) 802
SfcI CTRYAG 2 cut(s) 1555, 1786
Sfr274I CTCGAG 2 cut(s) 277, 1146
SfuI TTCGAA 1 cut(s) 910
SinI GGWCC 1 cut(s) 1781
SlaI CTCGAG 2 cut(s) 277, 1146
SmiMI CAYNNNNRTG 3 cut(s) 951, 1068, 1992
SmlI CTYRAG 3 cut(s) 277, 837, 1146
SmoI CTYRAG 3 cut(s) 277, 837, 1146
Sse9I AATT 9 cut(s) 355, 371, 400, 702, 823, 912, 979, 1111, 1762
SsiI CCGC 4 cut(s) 45, 1270, 1494, 1958
SspI AATATT 1 cut(s) 317
SspMI CTAG 5 cut(s) 300, 350, 416, 1332, 1680
SstI GAGCTC 1 cut(s) 284
StyD4I CCNGG 3 cut(s) 692, 966, 1726
StyI CCWWGG 2 cut(s) 1005, 1637
TaaI ACNGT 5 cut(s) 555, 659, 905, 989, 1018
TaiI ACGT 3 cut(s) 219, 1504, 1937
TaqI TCGA 6 cut(s) 278, 434, 883, 910, 1147, 1591
TasI AATT 9 cut(s) 355, 371, 400, 702, 823, 912, 979, 1111, 1762
TatI WGTACW 1 cut(s) 424
TauI GCSGC 1 cut(s) 1272
TfiI GAWTC 6 cut(s) 41, 173, 1283, 1318, 1739, 1832
Tru1I TTAA 2 cut(s) 861, 1434
Tru9I TTAA 2 cut(s) 861, 1434
TscAI CASTG 6 cut(s) 78, 958, 1052, 1129, 1832, 1868
TseFI GTSAC 2 cut(s) 302, 921
TseI GCWGC 2 cut(s) 272, 288
Tsp45I GTSAC 2 cut(s) 302, 921
TspDTI ATGAA 8 cut(s) 317, 384, 656, 749, 1108, 1293, 1310, 1316
TspGWI ACGGA 1 cut(s) 1397
TspRI CASTG 6 cut(s) 78, 958, 1052, 1129, 1832, 1868
VpaK11BI GGWCC 1 cut(s) 1781
XagI CCTNNNNNAGG 1 cut(s) 1641
XapI RAATTY 4 cut(s) 355, 400, 702, 979
XbaI TCTAGA 2 cut(s) 415, 1331
XceI RCATGY 2 cut(s) 1058, 1086
XcmI CCANNNNNNNNNTGG 1 cut(s) 938
XhoI CTCGAG 2 cut(s) 277, 1146
XmnI GAANNNNTTC 4 cut(s) 1238, 1299, 1766, 1818
XspI CTAG 5 cut(s) 300, 350, 416, 1332, 1680
Zsp2I ATGCAT 1 cut(s) 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.