RLG00000027801

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005653
Physical Location & Seq
Forward (+)
15372937 .. 15377270
4334 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027801

Sequence Viewer

Length: 639 bp
ATGACCTTTGTTGACGAACATTTTGTTGGGAACTCAGATCGCCGAACCAATCGACCCGAATCGAAATCCGGGTGTAATCTTTTCTGGTATAAATTTGTCTGGACGGACATCCGAGGAGAAGAAGTATCTGATATTGAAAGCTTCGATCCATATCAGGAATTGTTGAAAGCTTTGATCGGTTTCATAGATGTTGCTGGCCTTTACTTTGCTTTGACCCAGTTGACCCATAGGAACATCTGTCAAAATCATAAATTGCAAGCGCTATGGCTTTCATCTTCAATGGACAAAACTGTTAGATTATGGGATATGGCGACCAAGAGTTGTTTAAAAATGCTTGCCCATAATGATTATGTAACTTGCATACAGTTCAATCCTTTGGACGACAGTGTTTTTCTCAGCGGCTCCCTTGATGCGAAGTTTCGATTATGGGACATACCTGATCTAAGAATGGTAGACTGGACTGATCTTCATGAAATGGTCATTGCTGCATGCTATCCTCCAGAAGGCCAATTAACAGTTGACTTTGGTGGAAAAATAGTATGCTTCTTGTCTATTATTTTCCAATGTTACATGTCTAAAATAATTTTTTTCAGGGTTTCTCTTCCTTTCTCTGTTTCGGCCTTTCAGGGTATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

213

Amino Acids

24.47

Weight (kDa)

5.3

Isoelectric Point (pI)

38.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2053 PF09767 43 - 90 2.9e-07 Predicted membrane protein (DUF2053)
WD40_Prp19 PF24814 82 - 169 2.7e-08 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 85 - 169 3.6e-15 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 86 - 145 1.5e-09 WDR3 first beta-propeller domain
Beta-prop_Aladin PF25460 86 - 172 4.2e-08 Aladin seven-bladed propeller
Beta-prop_WDR3_2nd PF25172 88 - 158 4.1e-06 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 91 - 171 5.2e-12 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 453
AciI CCGC 1 cut(s) 399
AclWI GGATC 1 cut(s) 140
AcsI RAATTY 1 cut(s) 92
AfeI AGCGCT 1 cut(s) 261
AfiI CCNNNNNNNGG 1 cut(s) 503
AflIII ACRYGT 1 cut(s) 570
AgsI TTSAA 4 cut(s) 137, 166, 279, 370
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AluBI AGCT 2 cut(s) 141, 170
AluI AGCT 2 cut(s) 141, 170
AlwI GGATC 1 cut(s) 140
Aor51HI AGCGCT 1 cut(s) 261
AoxI GGCC 3 cut(s) 196, 505, 618
ApeKI GCWGC 1 cut(s) 485
ApoI RAATTY 1 cut(s) 92
ArsI GACNNNNNNTTYG 1 cut(s) 37
AspLEI GCGC 1 cut(s) 262
AsuC2I CCSGG 1 cut(s) 70
BbvI GCAGC 1 cut(s) 472
BcnI CCSGG 1 cut(s) 70
BfoI RGCGCY 1 cut(s) 263
BisI GCNGC 2 cut(s) 400, 486
BlsI GCNGC 2 cut(s) 401, 487
Bme1390I CCNGG 1 cut(s) 70
BmiI GGNNCC 1 cut(s) 403
BmrFI CCNGG 1 cut(s) 70
BmrI ACTGGG 1 cut(s) 211
BmsI GCATC 1 cut(s) 400
BmuI ACTGGG 1 cut(s) 211
BpmI CTGGAG 1 cut(s) 483
BpuMI CCSGG 1 cut(s) 70
BsaBI GATNNNNATC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 112
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 1 cut(s) 503
Bse1I ACTGG 2 cut(s) 217, 461
Bse3DI GCAATG 1 cut(s) 480
Bse8I GATNNNNATC 1 cut(s) 150
BseDI CCNNGG 1 cut(s) 112
BseGI GGATG 1 cut(s) 108
BseJI GATNNNNATC 1 cut(s) 150
BseLI CCNNNNNNNGG 1 cut(s) 503
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 2 cut(s) 48, 409
BseNI ACTGG 2 cut(s) 217, 461
BseRI GAGGAG 1 cut(s) 129
BseXI GCAGC 1 cut(s) 472
BshFI GGCC 3 cut(s) 198, 507, 620
BsiSI CCGG 1 cut(s) 69
BslFI GGGAC 1 cut(s) 443
BslI CCNNNNNNNGG 1 cut(s) 503
BsmFI GGGAC 1 cut(s) 443
BsnI GGCC 3 cut(s) 198, 507, 620
Bsp143I GATC 5 cut(s) 37, 145, 174, 439, 463
BspACI CCGC 1 cut(s) 399
BspANI GGCC 3 cut(s) 198, 507, 620
BspCNI CTCAG 2 cut(s) 47, 408
BspHI TCATGA 1 cut(s) 469
BspLI GGNNCC 1 cut(s) 403
BspPI GGATC 1 cut(s) 140
BsrDI GCAATG 1 cut(s) 480
BsrI ACTGG 2 cut(s) 217, 461
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 5 cut(s) 37, 145, 174, 439, 463
Bst4CI ACNGT 4 cut(s) 292, 366, 386, 517
Bst6I CTCTTC 1 cut(s) 606
BstC8I GCNNGC 4 cut(s) 196, 258, 336, 490
BstDEI CTNAG 3 cut(s) 34, 395, 443
BstENI CCTNNNNNAGG 1 cut(s) 501
BstF5I GGATG 1 cut(s) 108
BstH2I RGCGCY 1 cut(s) 263
BstHHI GCGC 1 cut(s) 262
BstKTI GATC 5 cut(s) 40, 148, 177, 442, 466
BstMBI GATC 5 cut(s) 37, 145, 174, 439, 463
BstNSI RCATGY 2 cut(s) 492, 574
BstSCI CCNGG 1 cut(s) 68
BstV1I GCAGC 1 cut(s) 472
BsuRI GGCC 3 cut(s) 198, 507, 620
BtsCI GGATG 1 cut(s) 108
BtsIMutI CAGTG 1 cut(s) 391
Cac8I GCNNGC 4 cut(s) 196, 258, 336, 490
CciI TCATGA 1 cut(s) 469
CfoI GCGC 1 cut(s) 262
CviAII CATG 3 cut(s) 470, 489, 571
CviJI RGCY 7 cut(s) 141, 170, 198, 268, 402, 507, 620
CviKI_1 RGCY 7 cut(s) 141, 170, 198, 268, 402, 507, 620
DdeI CTNAG 3 cut(s) 34, 395, 443
DpnI GATC 5 cut(s) 39, 147, 176, 441, 465
DpnII GATC 5 cut(s) 37, 145, 174, 439, 463
DraI TTTAAA 1 cut(s) 327
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Eco47III AGCGCT 1 cut(s) 261
EcoNI CCTNNNNNAGG 1 cut(s) 501
FaeI CATG 3 cut(s) 473, 492, 574
FaqI GGGAC 1 cut(s) 443
FatI CATG 3 cut(s) 469, 488, 570
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 2 cut(s) 400, 486
FokI GGATG 1 cut(s) 95
Fsp4HI GCNGC 2 cut(s) 400, 486
GlaI GCGC 1 cut(s) 261
GluI GCNGC 2 cut(s) 400, 486
GsuI CTGGAG 1 cut(s) 483
HaeII RGCGCY 1 cut(s) 263
HaeIII GGCC 3 cut(s) 198, 507, 620
HapII CCGG 1 cut(s) 69
HhaI GCGC 1 cut(s) 262
Hin1II CATG 3 cut(s) 473, 492, 574
Hin6I GCGC 1 cut(s) 260
HinP1I GCGC 1 cut(s) 260
HincII GTYRAC 3 cut(s) 13, 222, 520
HindII GTYRAC 3 cut(s) 13, 222, 520
HindIII AAGCTT 2 cut(s) 139, 168
HinfI GANTC 1 cut(s) 59
HpaII CCGG 1 cut(s) 69
Hpy166II GTNNAC 4 cut(s) 13, 222, 454, 520
Hpy188I TCNGA 3 cut(s) 37, 113, 130
Hpy188III TCNNGA 4 cut(s) 100, 155, 470, 500
Hpy8I GTNNAC 4 cut(s) 13, 222, 454, 520
HpyAV CCTTC 1 cut(s) 497
HpyCH4III ACNGT 4 cut(s) 292, 366, 386, 517
HpyCH4V TGCA 3 cut(s) 256, 360, 488
HpyF3I CTNAG 3 cut(s) 34, 395, 443
Hsp92II CATG 3 cut(s) 473, 492, 574
HspAI GCGC 1 cut(s) 260
Kzo9I GATC 5 cut(s) 37, 145, 174, 439, 463
LmnI GCTCC 1 cut(s) 407
Lsp1109I GCAGC 1 cut(s) 472
LweI GCATC 1 cut(s) 400
MaeIII GTNAC 2 cut(s) 352, 566
MalI GATC 5 cut(s) 39, 147, 176, 441, 465
MboI GATC 5 cut(s) 37, 145, 174, 439, 463
MboII GAAGA 4 cut(s) 131, 267, 458, 593
MluCI AATT 5 cut(s) 92, 158, 251, 509, 582
MnlI CCTC 2 cut(s) 107, 507
MseI TTAA 2 cut(s) 326, 512
MspA1I CMGCKG 1 cut(s) 399
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 1 cut(s) 70
NciI CCSGG 1 cut(s) 70
NdeII GATC 5 cut(s) 37, 145, 174, 439, 463
NlaIII CATG 3 cut(s) 473, 492, 574
NlaIV GGNNCC 1 cut(s) 403
NspI RCATGY 2 cut(s) 492, 574
PaeI GCATGC 1 cut(s) 492
PagI TCATGA 1 cut(s) 469
PciI ACATGT 1 cut(s) 570
PfeI GAWTC 1 cut(s) 59
PkrI GCNGC 2 cut(s) 401, 487
PscI ACATGT 1 cut(s) 570
PspN4I GGNNCC 1 cut(s) 403
SaqAI TTAA 2 cut(s) 326, 512
SatI GCNGC 2 cut(s) 400, 486
Sau3AI GATC 5 cut(s) 37, 145, 174, 439, 463
ScrFI CCNGG 1 cut(s) 70
SetI ASST 4 cut(s) 8, 143, 172, 439
SfaNI GCATC 1 cut(s) 400
SphI GCATGC 1 cut(s) 492
Sse9I AATT 5 cut(s) 92, 158, 251, 509, 582
SsiI CCGC 1 cut(s) 399
StyD4I CCNGG 1 cut(s) 68
TaaI ACNGT 4 cut(s) 292, 366, 386, 517
TaqI TCGA 4 cut(s) 52, 62, 144, 421
TasI AATT 5 cut(s) 92, 158, 251, 509, 582
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 2 cut(s) 326, 512
Tru9I TTAA 2 cut(s) 326, 512
TscAI CASTG 1 cut(s) 391
TseI GCWGC 1 cut(s) 485
TspDTI ATGAA 4 cut(s) 172, 261, 458, 486
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 1 cut(s) 391
XagI CCTNNNNNAGG 1 cut(s) 501
XapI RAATTY 1 cut(s) 92
XceI RCATGY 2 cut(s) 492, 574
XmiI GTMKAC 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.