RLG00000018526

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005653
Physical Location & Seq
Forward (+)
31330946 .. 31334468
3523 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018526

Sequence Viewer

Length: 2067 bp
ATGCTGAGTTCTGATGGGGGTGAGAAGGATTTGTTCTTTGATTCCGCAGGGTATCTGTCATCTGAAGAGTCAGTGGTAGTGAAGGCGGAGCTGGGGTGTAGCACGTTGGAGTATGAGATTTGGTTGAATGAGCCACCGAGTGTGAAGGAAAGACGAGAGAGTTTTCTACGGCAGATGGGTTTAGCTGAATCTGGATCTACAAAGTTTGGTTCTGTGGAGATGGAAAGGGACGTTGGTACATCATCGGAGATGATGGGATTGGAGAGACTTTTGGAGTGGAGTGGAGCTGCCTCGAGCTCTTACAGCTCCTCCTCTAGTCACATTGAGGAATATTCGGGTTTTCATAGCAGGGAAGGGGACAATCTAGCAAATTTTATGTATGATGAATTGGACAGAGACCTGCAAGACAAGTCAAATTTAGTTTCCATTCTAGAGTCAGTACAAGTTTCAACTTCTGGTAGTGATTGTTCTCAGGGGGAAACTTGTTCTCAGAGAGAAACTTCTTCTCAGAGAGAAACTGAGGCTACTGAAGAAGAATGCGAGAGTTCAAAGGAGGGCCGGAAGAAGAAGAGAAGTTGGTGGAAACAGTTTATAAACAAGAGCAAGAGGAGAGGTGGTAAAGTTGTCTCAGAGGTATCAAAACCTGATACAGAAGTATCCAAAGAAAACAGAATGAAGATCAAACAGAACAGTAAGAGGTACATGGAGTTTGCTGCACTTTGCACCGGGCAAGAAATTCAGGCTCACAATGGGTTTATTTGGACCATGAAGTTTAGTCCTGATGGCCAATACATTGCAACTGGTGGTGAAGATGGGGTTGTACGCATTTGGTGTGTTACAGCAGATGCCTCATGTAATTACCTTATGGCTCAAGGCAACTTAGATAGCAAACTTAAAAAGATCAAGTCTGGATGTCGAGTTATTTTTCCTGACAACAGTTTTCGAATTGAAGAGTCACCTCTCCAAGAGTTTCTTGGCCATTCCAGTGATGTGTTAGACCTGGCTTGGTCAAATTCAAACCGTCTCATTTCTTCGTCCATGGATAAAACTGTTCGTCTGTGGGAAGTTGGTTGCAGTCAGTGCCTACATGTTTTCCATCACAATGACTATGTAACATGCATTCAGTTCAATCCTCTCGATGACAATTACTTCATCAGTGGATCCATAGATGGAAAGGTTCGAGTTTGGGGGCTTTCTGAGAAGCGAGTTGTTGACTGGGCTGATGTGCGAGATGTCATAACAGCTATTTGTTATCAACCAGATGGGAAGGGTTTCGCAGTTGGTTCCCTTGCAGGCACTTGCCGCTTCTATGAAGAATCAGGAAAAGAAGCTCTTCAGCTTGTTGCACAGATTCATATTTGTTCTAGAAGGAAGACCTCTGGCAACAAAATCACTGGCATCCAGTTTTCCGAGGAAAATGCTCAAAGAGTTATGATAACATCAGAAGACTCAAAAGTCCGTGTGTTTGATGGGGTAGATCTCATTAAGAAATACAGAGGTCTTCCAAAGTCAGGAAGTCAAATGTCAGCTTCTTTCACATCCAGCGGGAAACGTATAATCTCAGTTGGAGAGGACTCGCGCGTTTATTTATGGGACTATGACAGCTGTTGTCTTCCATCATCCAAGCATACAAAATCCATTCGATCCTGCGAACATTTCTATTGTGAAGGTGTGTCTGTTGCAATCCCTTGGCCAGGCATGGGAACTGAAAATAGGAACTTAGAAACTGCTAGTCCACAATATTGCTCACAAACACAAGGCCGAATAGACTCTACTCCTGGCAGTAGAGATTCGGAACGCTTTTTGCTTGGGAATTGGTTCTTTCTGGAGGGTCCCTGTAGAGGCTCTGCAACTTGGCCCGAGGAAAACCTTCCTCAGTGGGATTCACCAGTGGCAGGAGAGGAAGATGATCACTGGCAATACAAAGACCATCAACACCATCACCACCACAATAAAGCTCACAACCAGTGGCCGAAATCAGAAACGTGGGGGCTTGTCATTGTAACAGCCGGATGGGATGGAAAAATCAGGACATTCCATAACTATGGATTGCCGGTCACGTTGTAG

Protein Analysis

689

Amino Acids

77.39

Weight (kDa)

5.57

Isoelectric Point (pI)

49.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 229 - 404 9.4e-16 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 233 - 280 2.3e-07 THOC3 beta-propeller domain
WD40 PF00400 245 - 277 2.3e-09 WD domain, G-beta repeat
Beta-prop_IFT122_1st PF23381 303 - 446 2.7e-07 IFT122 first beta-propeller
WD40_CDC20-Fz PF24807 314 - 423 8.8e-11 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 317 - 397 8.5e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 318 - 549 2.1e-21 Prp19 WD40 domain
WD40 PF00400 319 - 354 2.5e-08 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 320 - 535 2.5e-34 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 320 - 364 1.3e-06 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 322 - 381 1.5e-08 WDR36/Utp21 second beta-propeller domain
WDR55 PF24796 323 - 433 1.4e-06 WDR55
Beta-prop_THOC3 PF25174 327 - 558 6.8e-26 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 329 - 423 6.7e-06 Aladin seven-bladed propeller
Beta-prop_WDR3_2nd PF25172 330 - 537 3e-12 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 333 - 455 5.1e-11 G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd PF23393 339 - 491 2.1e-10 WDR90/POC16, second beta-propeller
WD40 PF00400 360 - 396 1.9e-06 WD domain, G-beta repeat
WD40_WDHD1_1st PF24817 371 - 548 1.3e-09 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 381 - 550 8.5e-15 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 401 - 549 1.2e-08 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 593
AasI GACNNNNNNGTC 1 cut(s) 1454
Acc36I ACCTGC 1 cut(s) 408
AccII CGCG 2 cut(s) 1579, 1581
AciI CCGC 4 cut(s) 45, 86, 1303, 1545
AclWI GGATC 4 cut(s) 202, 1155, 1168, 1638
AcoI YGGCCR 4 cut(s) 784, 976, 1691, 1970
AcsI RAATTY 4 cut(s) 370, 415, 735, 1012
AcuI CTGAAG 3 cut(s) 84, 549, 1319
AdeI CACNNNGTG 1 cut(s) 140
AfaI GTAC 4 cut(s) 238, 441, 701, 822
AfiI CCNNNNNNNGG 5 cut(s) 1511, 1694, 1700, 1841, 1895
AflIII ACRYGT 1 cut(s) 1087
AgsI TTSAA 6 cut(s) 127, 450, 549, 950, 1017, 1129
AjnI CCWGG 3 cut(s) 999, 1693, 1777
AjuI GAANNNNNNNTTGG 2 cut(s) 216, 248
Alw21I GWGCWC 1 cut(s) 299
Alw26I GTCTC 4 cut(s) 259, 390, 631, 1028
AlwI GGATC 4 cut(s) 202, 1155, 1168, 1638
Ama87I CYCGRG 2 cut(s) 292, 1859
AoxI GGCC 7 cut(s) 556, 784, 976, 1691, 1759, 1856, 1970
ApeKI GCWGC 2 cut(s) 287, 713
ApoI RAATTY 4 cut(s) 370, 415, 735, 1012
Asp700I GAANNNNTTC 4 cut(s) 1271, 1332, 1817, 1869
AspLEI GCGC 1 cut(s) 1581
AspS9I GGNCC 4 cut(s) 556, 762, 1832, 1857
AsuC2I CCSGG 1 cut(s) 727
AsuHPI GGTGA 5 cut(s) 32, 818, 948, 1878, 1934
AsuII TTCGAA 1 cut(s) 943
AvaI CYCGRG 2 cut(s) 292, 1859
AvaII GGWCC 2 cut(s) 762, 1832
BaeI ACNNNNGTAYC 4 cut(s) 639, 639, 672, 672
BalI TGGCCA 3 cut(s) 786, 978, 1693
BamHI GGATCC 1 cut(s) 1160
BanII GRGCYC 1 cut(s) 299
BbsI GAAGAC 4 cut(s) 1379, 1452, 1493, 1604
Bbv12I GWGCWC 1 cut(s) 299
BbvI GCAGC 2 cut(s) 274, 700
BceAI ACGGC 1 cut(s) 185
BcgI CGANNNNNNTGC 2 cut(s) 1400, 1434
BciT130I CCWGG 3 cut(s) 1001, 1695, 1779
BciVI GTATCC 1 cut(s) 667
BclI TGATCA 1 cut(s) 1909
BcnI CCSGG 1 cut(s) 727
BcoDI GTCTC 4 cut(s) 259, 390, 631, 1028
BfaI CTAG 5 cut(s) 315, 365, 431, 1365, 1731
BfmI CTRYAG 1 cut(s) 1837
BfuAI ACCTGC 1 cut(s) 408
BfuI GTATCC 1 cut(s) 667
BglII AGATCT 1 cut(s) 1477
BisI GCNGC 3 cut(s) 288, 714, 1303
BlsI GCNGC 3 cut(s) 289, 715, 1304
Bme1390I CCNGG 4 cut(s) 727, 1001, 1695, 1779
Bme18I GGWCC 2 cut(s) 762, 1832
BmeT110I CYCGRG 2 cut(s) 292, 1859
BmgT120I GGNCC 4 cut(s) 556, 762, 1832, 1857
BmiI GGNNCC 4 cut(s) 1162, 1285, 1833, 1834
BmrFI CCNGG 4 cut(s) 727, 1001, 1695, 1779
BmrI ACTGGG 1 cut(s) 1225
BmsI GCATC 2 cut(s) 835, 1407
BmuI ACTGGG 1 cut(s) 1225
BpiI GAAGAC 4 cut(s) 1379, 1452, 1493, 1604
BpmI CTGGAG 1 cut(s) 1847
Bpu14I TTCGAA 1 cut(s) 943
BpuEI CTTGAG 1 cut(s) 855
BpuMI CCSGG 1 cut(s) 727
BsaI GGTCTC 1 cut(s) 390
BsaJI CCNNGG 4 cut(s) 1038, 1410, 1688, 1860
BsaXI ACNNNNNCTCC 6 cut(s) 80, 110, 293, 323, 601, 631
Bsc4I CCNNNNNNNGG 5 cut(s) 1511, 1694, 1700, 1841, 1895
Bse118I RCCGGY 1 cut(s) 2053
Bse1I ACTGG 8 cut(s) 805, 984, 1220, 1399, 1402, 1889, 1919, 1966
Bse3DI GCAATG 1 cut(s) 792
BseBI CCWGG 3 cut(s) 1001, 1695, 1779
BseDI CCNNGG 4 cut(s) 1038, 1410, 1688, 1860
BseGI GGATG 6 cut(s) 917, 1398, 1538, 1619, 2018, 2023
BseLI CCNNNNNNNGG 5 cut(s) 1511, 1694, 1700, 1841, 1895
BseMI GCAATG 1 cut(s) 792
BseMII CTCAG 8 cut(s) 485, 503, 510, 521, 642, 1188, 1575, 1889
BseNI ACTGG 8 cut(s) 805, 984, 1220, 1399, 1402, 1889, 1919, 1966
BseRI GAGGAG 3 cut(s) 298, 301, 622
BseXI GCAGC 2 cut(s) 274, 700
BseYI CCCAGC 1 cut(s) 91
BsgI GTGCAG 1 cut(s) 699
Bsh1236I CGCG 2 cut(s) 1579, 1581
BshFI GGCC 7 cut(s) 558, 786, 978, 1693, 1761, 1858, 1972
BsiHKAI GWGCWC 1 cut(s) 299
BsiHKCI CYCGRG 2 cut(s) 292, 1859
BsiSI CCGG 4 cut(s) 559, 726, 2010, 2054
BslFI GGGAC 4 cut(s) 242, 371, 1607, 1818
BslI CCNNNNNNNGG 5 cut(s) 1511, 1694, 1700, 1841, 1895
BsmAI GTCTC 4 cut(s) 259, 390, 631, 1028
BsmBI CGTCTC 1 cut(s) 1028
BsmFI GGGAC 4 cut(s) 242, 371, 1607, 1818
BsmI GAATGC 2 cut(s) 542, 1119
BsnI GGCC 7 cut(s) 558, 786, 978, 1693, 1761, 1858, 1972
Bso31I GGTCTC 1 cut(s) 390
BsoBI CYCGRG 2 cut(s) 292, 1859
Bsp119I TTCGAA 1 cut(s) 943
Bsp1286I GDGCHC 1 cut(s) 299
Bsp143I GATC 7 cut(s) 194, 678, 900, 1160, 1477, 1643, 1909
Bsp19I CCATGG 1 cut(s) 1038
BspACI CCGC 4 cut(s) 45, 86, 1303, 1545
BspANI GGCC 7 cut(s) 558, 786, 978, 1693, 1761, 1858, 1972
BspCNI CTCAG 8 cut(s) 484, 502, 511, 520, 641, 1189, 1574, 1888
BspFNI CGCG 2 cut(s) 1579, 1581
BspLI GGNNCC 4 cut(s) 1162, 1285, 1833, 1834
BspMI ACCTGC 1 cut(s) 408
BspPI GGATC 4 cut(s) 202, 1155, 1168, 1638
BspQI GCTCTTC 1 cut(s) 1338
BspT104I TTCGAA 1 cut(s) 943
BspTNI GGTCTC 1 cut(s) 390
BsrDI GCAATG 1 cut(s) 792
BsrFI RCCGGY 1 cut(s) 2053
BsrI ACTGG 8 cut(s) 805, 984, 1220, 1399, 1402, 1889, 1919, 1966
BssAI RCCGGY 1 cut(s) 2053
BssECI CCNNGG 4 cut(s) 1038, 1410, 1688, 1860
BssMI GATC 7 cut(s) 194, 678, 900, 1160, 1477, 1643, 1909
BssT1I CCWWGG 2 cut(s) 1038, 1688
Bst2UI CCWGG 3 cut(s) 1001, 1695, 1779
Bst4CI ACNGT 5 cut(s) 588, 692, 938, 1022, 1051
Bst6I CTCTTC 4 cut(s) 60, 563, 945, 1338
BstAPI GCANNNNNTGC 1 cut(s) 1080
BstBI TTCGAA 1 cut(s) 943
BstC8I GCNNGC 1 cut(s) 1294
BstDSI CCRYGG 1 cut(s) 1038
BstENI CCTNNNNNAGG 1 cut(s) 1692
BstF5I GGATG 6 cut(s) 917, 1398, 1538, 1619, 2018, 2023
BstFNI CGCG 2 cut(s) 1579, 1581
BstHHI GCGC 1 cut(s) 1581
BstKTI GATC 7 cut(s) 197, 681, 903, 1163, 1480, 1646, 1912
BstMAI GTCTC 4 cut(s) 259, 390, 631, 1028
BstMBI GATC 7 cut(s) 194, 678, 900, 1160, 1477, 1643, 1909
BstMWI GCNNNNNNNGC 3 cut(s) 303, 1080, 1302
BstNI CCWGG 3 cut(s) 1001, 1695, 1779
BstNSI RCATGY 2 cut(s) 1091, 1119
BstSCI CCNGG 4 cut(s) 725, 999, 1693, 1777
BstSFI CTRYAG 1 cut(s) 1837
BstUI CGCG 2 cut(s) 1579, 1581
BstV1I GCAGC 2 cut(s) 274, 700
BstV2I GAAGAC 4 cut(s) 1379, 1452, 1493, 1604
BstX2I RGATCY 3 cut(s) 194, 1160, 1477
BstXI CCANNNNNNTGG 1 cut(s) 2045
BstYI RGATCY 3 cut(s) 194, 1160, 1477
BsuI GTATCC 1 cut(s) 667
BsuRI GGCC 7 cut(s) 558, 786, 978, 1693, 1761, 1858, 1972
BtgI CCRYGG 1 cut(s) 1038
BtsCI GGATG 6 cut(s) 917, 1398, 1538, 1619, 2018, 2023
BtsIMutI CAGTG 9 cut(s) 78, 991, 1085, 1162, 1392, 1883, 1896, 1912, 1973
BveI ACCTGC 1 cut(s) 408
Cac8I GCNNGC 1 cut(s) 1294
CfoI GCGC 1 cut(s) 1581
Cfr10I RCCGGY 1 cut(s) 2053
Cfr13I GGNCC 4 cut(s) 556, 762, 1832, 1857
Csp6I GTAC 4 cut(s) 237, 440, 700, 821
CviAII CATG 7 cut(s) 703, 766, 852, 1039, 1088, 1116, 1699
CviQI GTAC 4 cut(s) 237, 440, 700, 821
DpnI GATC 7 cut(s) 196, 680, 902, 1162, 1479, 1645, 1911
DpnII GATC 7 cut(s) 194, 678, 900, 1160, 1477, 1643, 1909
DraIII CACNNNGTG 1 cut(s) 140
DrdI GACNNNNNNGTC 1 cut(s) 1454
DseDI GACNNNNNNGTC 1 cut(s) 1454
EaeI YGGCCR 4 cut(s) 784, 976, 1691, 1970
Eam1104I CTCTTC 4 cut(s) 60, 563, 945, 1338
EarI CTCTTC 4 cut(s) 60, 563, 945, 1338
EciI GGCGGA 1 cut(s) 101
Ecl136II GAGCTC 1 cut(s) 297
Eco130I CCWWGG 2 cut(s) 1038, 1688
Eco24I GRGCYC 1 cut(s) 299
Eco31I GGTCTC 1 cut(s) 390
Eco47I GGWCC 2 cut(s) 762, 1832
Eco53kI GAGCTC 1 cut(s) 297
Eco57I CTGAAG 3 cut(s) 84, 549, 1319
Eco88I CYCGRG 2 cut(s) 292, 1859
EcoICRI GAGCTC 1 cut(s) 297
EcoNI CCTNNNNNAGG 1 cut(s) 1692
EcoO109I RGGNCCY 1 cut(s) 1832
EcoRII CCWGG 3 cut(s) 999, 1693, 1777
EcoT14I CCWWGG 2 cut(s) 1038, 1688
EcoT22I ATGCAT 1 cut(s) 1121
EcoT38I GRGCYC 1 cut(s) 299
ErhI CCWWGG 2 cut(s) 1038, 1688
Esp3I CGTCTC 1 cut(s) 1028
FaeI CATG 7 cut(s) 706, 769, 855, 1042, 1091, 1119, 1702
FalI AAGNNNNNCTT 4 cut(s) 957, 989, 1317, 1349
FaqI GGGAC 4 cut(s) 242, 371, 1607, 1818
FatI CATG 7 cut(s) 702, 765, 851, 1038, 1087, 1115, 1698
FauI CCCGC 1 cut(s) 1538
FbaI TGATCA 1 cut(s) 1909
Fnu4HI GCNGC 3 cut(s) 288, 714, 1303
FokI GGATG 6 cut(s) 924, 1385, 1525, 1606, 2025, 2030
FriOI GRGCYC 1 cut(s) 299
Fsp4HI GCNGC 3 cut(s) 288, 714, 1303
FspBI CTAG 5 cut(s) 315, 365, 431, 1365, 1731
GlaI GCGC 1 cut(s) 1580
GluI GCNGC 3 cut(s) 288, 714, 1303
GsaI CCCAGC 1 cut(s) 95
GsuI CTGGAG 1 cut(s) 1847
HaeIII GGCC 7 cut(s) 558, 786, 978, 1693, 1761, 1858, 1972
HapII CCGG 4 cut(s) 559, 726, 2010, 2054
HhaI GCGC 1 cut(s) 1581
Hin1II CATG 7 cut(s) 706, 769, 855, 1042, 1091, 1119, 1702
Hin6I GCGC 1 cut(s) 1579
HinP1I GCGC 1 cut(s) 1579
HincII GTYRAC 1 cut(s) 1213
HindII GTYRAC 1 cut(s) 1213
HpaII CCGG 4 cut(s) 559, 726, 2010, 2054
HphI GGTGA 5 cut(s) 32, 818, 948, 1878, 1934
Hpy166II GTNNAC 2 cut(s) 1213, 1736
Hpy8I GTNNAC 2 cut(s) 1213, 1736
HpyAV CCTTC 8 cut(s) 19, 76, 139, 347, 1261, 1362, 1661, 1880
HpyCH4III ACNGT 5 cut(s) 588, 692, 938, 1022, 1051
HpyCH4IV ACGT 5 cut(s) 104, 231, 1552, 1985, 2060
HpyF10VI GCNNNNNNNGC 3 cut(s) 303, 1080, 1302
HpySE526I ACGT 5 cut(s) 104, 231, 1552, 1985, 2060
Hsp92II CATG 7 cut(s) 706, 769, 855, 1042, 1091, 1119, 1702
HspAI GCGC 1 cut(s) 1579
KflI GGGWCCC 1 cut(s) 1832
Ksp22I TGATCA 1 cut(s) 1909
Kzo9I GATC 7 cut(s) 194, 678, 900, 1160, 1477, 1643, 1909
LguI GCTCTTC 1 cut(s) 1338
LmnI GCTCC 3 cut(s) 88, 284, 311
Lsp1109I GCAGC 2 cut(s) 274, 700
LweI GCATC 2 cut(s) 835, 1407
MaeI CTAG 5 cut(s) 315, 365, 431, 1365, 1731
MaeII ACGT 5 cut(s) 104, 231, 1552, 1985, 2060
MaeIII GTNAC 6 cut(s) 317, 835, 954, 1111, 2002, 2056
MalI GATC 7 cut(s) 196, 680, 902, 1162, 1479, 1645, 1911
MboI GATC 7 cut(s) 194, 678, 900, 1160, 1477, 1643, 1909
MflI RGATCY 3 cut(s) 194, 1160, 1477
MhlI GDGCHC 1 cut(s) 299
MlsI TGGCCA 3 cut(s) 786, 978, 1693
MluCI AATT 9 cut(s) 370, 386, 415, 735, 856, 945, 1012, 1144, 1813
MluNI TGGCCA 3 cut(s) 786, 978, 1693
MlyI GAGTC 6 cut(s) 77, 443, 962, 1442, 1568, 1763
MmeI TCCRAC 2 cut(s) 87, 1546
Mox20I TGGCCA 3 cut(s) 786, 978, 1693
Mph1103I ATGCAT 1 cut(s) 1121
MroXI GAANNNNTTC 4 cut(s) 1271, 1332, 1817, 1869
MscI TGGCCA 3 cut(s) 786, 978, 1693
MseI TTAA 2 cut(s) 894, 1485
MslI CAYNNNNRTG 3 cut(s) 984, 1101, 2043
Msp20I TGGCCA 3 cut(s) 786, 978, 1693
MspA1I CMGCKG 2 cut(s) 1545, 1605
MspI CCGG 4 cut(s) 559, 726, 2010, 2054
MspR9I CCNGG 4 cut(s) 727, 1001, 1695, 1779
Mva1269I GAATGC 2 cut(s) 542, 1119
MvaI CCWGG 3 cut(s) 1001, 1695, 1779
MvnI CGCG 2 cut(s) 1579, 1581
MwoI GCNNNNNNNGC 3 cut(s) 303, 1080, 1302
NciI CCSGG 1 cut(s) 727
NcoI CCATGG 1 cut(s) 1038
NdeII GATC 7 cut(s) 194, 678, 900, 1160, 1477, 1643, 1909
NlaIII CATG 7 cut(s) 706, 769, 855, 1042, 1091, 1119, 1702
NlaIV GGNNCC 4 cut(s) 1162, 1285, 1833, 1834
NmuCI GTSAC 3 cut(s) 317, 954, 2056
NsiI ATGCAT 1 cut(s) 1121
NspI RCATGY 2 cut(s) 1091, 1119
NspV TTCGAA 1 cut(s) 943
PaeR7I CTCGAG 1 cut(s) 292
PciI ACATGT 1 cut(s) 1087
PciSI GCTCTTC 1 cut(s) 1338
PctI GAATGC 2 cut(s) 542, 1119
PdmI GAANNNNTTC 4 cut(s) 1271, 1332, 1817, 1869
PfeI GAWTC 6 cut(s) 41, 188, 1316, 1351, 1790, 1883
PkrI GCNGC 3 cut(s) 289, 715, 1304
PleI GAGTC 6 cut(s) 76, 442, 961, 1442, 1568, 1763
PpsI GAGTC 6 cut(s) 76, 442, 961, 1442, 1568, 1763
PpuMI RGGWCCY 1 cut(s) 1832
PscI ACATGT 1 cut(s) 1087
PsiI TTATAA 1 cut(s) 593
Psp124BI GAGCTC 1 cut(s) 299
Psp5II RGGWCCY 1 cut(s) 1832
Psp6I CCWGG 3 cut(s) 999, 1693, 1777
PspFI CCCAGC 1 cut(s) 91
PspGI CCWGG 3 cut(s) 999, 1693, 1777
PspN4I GGNNCC 4 cut(s) 1162, 1285, 1833, 1834
PspPI GGNCC 4 cut(s) 556, 762, 1832, 1857
PspPPI RGGWCCY 1 cut(s) 1832
PspXI VCTCGAGB 1 cut(s) 292
PsrI GAACNNNNNNTAC 2 cut(s) 451, 483
PsuI RGATCY 3 cut(s) 194, 1160, 1477
PvuII CAGCTG 1 cut(s) 1605
RsaI GTAC 4 cut(s) 238, 441, 701, 822
RsaNI GTAC 4 cut(s) 237, 440, 700, 821
RseI CAYNNNNRTG 3 cut(s) 984, 1101, 2043
SacI GAGCTC 1 cut(s) 299
SapI GCTCTTC 1 cut(s) 1338
SaqAI TTAA 2 cut(s) 894, 1485
SatI GCNGC 3 cut(s) 288, 714, 1303
Sau3AI GATC 7 cut(s) 194, 678, 900, 1160, 1477, 1643, 1909
Sau96I GGNCC 4 cut(s) 556, 762, 1832, 1857
SchI GAGTC 6 cut(s) 77, 443, 962, 1442, 1568, 1763
ScrFI CCNGG 4 cut(s) 727, 1001, 1695, 1779
SduI GDGCHC 1 cut(s) 299
SfaNI GCATC 2 cut(s) 835, 1407
SfcI CTRYAG 1 cut(s) 1837
Sfr274I CTCGAG 1 cut(s) 292
SfuI TTCGAA 1 cut(s) 943
SinI GGWCC 2 cut(s) 762, 1832
SlaI CTCGAG 1 cut(s) 292
SmiMI CAYNNNNRTG 3 cut(s) 984, 1101, 2043
SmlI CTYRAG 2 cut(s) 292, 870
SmoI CTYRAG 2 cut(s) 292, 870
Sse9I AATT 9 cut(s) 370, 386, 415, 735, 856, 945, 1012, 1144, 1813
SsiI CCGC 4 cut(s) 45, 86, 1303, 1545
SspI AATATT 2 cut(s) 332, 1742
SspMI CTAG 5 cut(s) 315, 365, 431, 1365, 1731
SstI GAGCTC 1 cut(s) 299
StyD4I CCNGG 4 cut(s) 725, 999, 1693, 1777
StyI CCWWGG 2 cut(s) 1038, 1688
TaaI ACNGT 5 cut(s) 588, 692, 938, 1022, 1051
TaiI ACGT 5 cut(s) 107, 234, 1555, 1988, 2063
TaqI TCGA 6 cut(s) 293, 916, 943, 1137, 1180, 1642
TasI AATT 9 cut(s) 370, 386, 415, 735, 856, 945, 1012, 1144, 1813
TatI WGTACW 1 cut(s) 439
TauI GCSGC 1 cut(s) 1305
TfiI GAWTC 6 cut(s) 41, 188, 1316, 1351, 1790, 1883
Tru1I TTAA 2 cut(s) 894, 1485
Tru9I TTAA 2 cut(s) 894, 1485
TscAI CASTG 9 cut(s) 78, 991, 1085, 1162, 1399, 1883, 1896, 1919, 1973
TseFI GTSAC 3 cut(s) 317, 954, 2056
TseI GCWGC 2 cut(s) 287, 713
Tsp45I GTSAC 3 cut(s) 317, 954, 2056
TspDTI ATGAA 7 cut(s) 332, 399, 689, 782, 1141, 1326, 1343
TspGWI ACGGA 1 cut(s) 1448
TspRI CASTG 9 cut(s) 78, 991, 1085, 1162, 1399, 1883, 1896, 1919, 1973
VpaK11BI GGWCC 2 cut(s) 762, 1832
XagI CCTNNNNNAGG 1 cut(s) 1692
XapI RAATTY 4 cut(s) 370, 415, 735, 1012
XbaI TCTAGA 2 cut(s) 430, 1364
XceI RCATGY 2 cut(s) 1091, 1119
XcmI CCANNNNNNNNNTGG 1 cut(s) 971
XhoI CTCGAG 1 cut(s) 292
XmnI GAANNNNTTC 4 cut(s) 1271, 1332, 1817, 1869
XspI CTAG 5 cut(s) 315, 365, 431, 1365, 1731
Zsp2I ATGCAT 1 cut(s) 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.