MD15G1338400.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005653
Physical Location & Seq
Reverse (-)
38151423 .. 38153019
1597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1338400.v1.1.491

Sequence Viewer

Length: 618 bp
ATGCGCACTGGTATTGAGATTCAGTTCAACAAAACTTATCTTGCAACTGGTGGTGAAGATGGGGTTGTACGCATCTGGCATGTTACATTAGTAGATGCCTCTAGTAACTACTTAACACCTCAAGGGATATTTGATAGCAAACTTAAGAAAATAAAGTACGGATTTCAAGTGATCTTTCCTGAAAAGGGTTTTCGAATTCAAGAGTCACCTCTGCAAGAGTTTCATGGCCATTCCAGTGATGTGTTAGACCTGGCTTGGTCGAATTCAAATTGTCTTGTTTCTTCGTCCATGGATAAAACTGTTCGTCTGTGGGAAGTTGGTCGCAGTCACTGCCTATATATTTTCCATCACAATGACTATGATGGGAAAGTTCGAGTTTGGGGACCTTCTGAGAAGCGAGTTGGTTTCGTGGTTGGTTCCGTTACAGGCACTTGCCACTTCTATGAAGTATCAAGGGATTATCTTCAGCTGGTTGTGCGAATGCATATTCATGGTAGGAAGAAAACCTCCAGCAACCAAATCACCGGAGTCAAGGTTTTACACTCAATGCTCTTTCGACCTTTTCTTTTCCACCGAGCTTCTATAAGATCTAATTCATTCGTTAACACTTACGTTTAA

Protein Analysis

206

Amino Acids

23.52

Weight (kDa)

9.49

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR36-Utp21_2nd PF25168 3 - 117 4e-08 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 66 - 149 5.4e-09 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 68 - 154 4.2e-07 Prp19 WD40 domain
WD40 PF00400 69 - 104 2.5e-09 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 70 - 133 3.9e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 70 - 152 2.7e-08 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 73 - 188 4.1e-10 CAF1B/HIR1 beta-propeller domain
WD40_Gbeta PF25391 73 - 154 1e-05 G protein beta WD-40 repeat protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
AcoI YGGCCR 1 cut(s) 226
AcsI RAATTY 2 cut(s) 195, 262
AcuI CTGAAG 1 cut(s) 449
AfaI GTAC 2 cut(s) 69, 158
AfiI CCNNNNNNNGG 1 cut(s) 185
AflII CTTAAG 1 cut(s) 143
AgsI TTSAA 4 cut(s) 28, 167, 200, 267
AjnI CCWGG 1 cut(s) 249
AluBI AGCT 2 cut(s) 469, 578
AluI AGCT 2 cut(s) 469, 578
AlwNI CAGNNNCTG 1 cut(s) 330
AoxI GGCC 1 cut(s) 226
ApoI RAATTY 2 cut(s) 195, 262
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 1 cut(s) 383
AsuHPI GGTGA 3 cut(s) 65, 198, 514
AsuII TTCGAA 1 cut(s) 193
AvaII GGWCC 1 cut(s) 383
BalI TGGCCA 1 cut(s) 228
BccI CCATC 3 cut(s) 53, 354, 356
BciT130I CCWGG 1 cut(s) 251
BfaI CTAG 1 cut(s) 102
BfrI CTTAAG 1 cut(s) 143
BglII AGATCT 1 cut(s) 587
Bme1390I CCNGG 1 cut(s) 251
Bme18I GGWCC 1 cut(s) 383
BmgT120I GGNCC 1 cut(s) 383
BmiI GGNNCC 2 cut(s) 384, 418
BmrFI CCNGG 1 cut(s) 251
BmsI GCATC 2 cut(s) 81, 85
BpmI CTGGAG 1 cut(s) 493
Bpu14I TTCGAA 1 cut(s) 193
BpuEI CTTGAG 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 288
BsaWI WCCGGW 1 cut(s) 524
BsaXI ACNNNNNCTCC 2 cut(s) 519, 549
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse1I ACTGG 3 cut(s) 13, 52, 234
BseBI CCWGG 1 cut(s) 251
BseDI CCNNGG 1 cut(s) 288
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 3 cut(s) 13, 52, 234
BshFI GGCC 1 cut(s) 228
BsiSI CCGG 1 cut(s) 525
BslFI GGGAC 1 cut(s) 396
BslI CCNNNNNNNGG 1 cut(s) 185
BsmFI GGGAC 1 cut(s) 396
BsmI GAATGC 1 cut(s) 486
BsnI GGCC 1 cut(s) 228
Bsp119I TTCGAA 1 cut(s) 193
Bsp143I GATC 2 cut(s) 171, 587
Bsp19I CCATGG 1 cut(s) 288
BspANI GGCC 1 cut(s) 228
BspCNI CTCAG 1 cut(s) 382
BspLI GGNNCC 2 cut(s) 384, 418
BspT104I TTCGAA 1 cut(s) 193
BspTI CTTAAG 1 cut(s) 143
BsrI ACTGG 3 cut(s) 13, 52, 234
BssECI CCNNGG 1 cut(s) 288
BssMI GATC 2 cut(s) 171, 587
BssT1I CCWWGG 1 cut(s) 288
Bst2UI CCWGG 1 cut(s) 251
Bst4CI ACNGT 1 cut(s) 301
BstAFI CTTAAG 1 cut(s) 143
BstAPI GCANNNNNTGC 1 cut(s) 330
BstBI TTCGAA 1 cut(s) 193
BstDEI CTNAG 1 cut(s) 390
BstDSI CCRYGG 1 cut(s) 288
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 2 cut(s) 174, 590
BstMBI GATC 2 cut(s) 171, 587
BstMWI GCNNNNNNNGC 2 cut(s) 330, 475
BstNI CCWGG 1 cut(s) 251
BstNSI RCATGY 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 249
BstX2I RGATCY 1 cut(s) 587
BstYI RGATCY 1 cut(s) 587
BsuRI GGCC 1 cut(s) 228
BtgI CCRYGG 1 cut(s) 288
BtsI GCAGTG 1 cut(s) 328
BtsIMutI CAGTG 3 cut(s) 6, 241, 328
CaiI CAGNNNCTG 1 cut(s) 330
CfoI GCGC 1 cut(s) 6
Cfr13I GGNCC 1 cut(s) 383
Csp6I GTAC 2 cut(s) 68, 157
CviAII CATG 4 cut(s) 80, 224, 289, 491
CviJI RGCY 4 cut(s) 228, 254, 469, 578
CviKI_1 RGCY 4 cut(s) 228, 254, 469, 578
CviQI GTAC 2 cut(s) 68, 157
DdeI CTNAG 1 cut(s) 390
DpnI GATC 2 cut(s) 173, 589
DpnII GATC 2 cut(s) 171, 587
EaeI YGGCCR 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 288
Eco47I GGWCC 1 cut(s) 383
Eco57I CTGAAG 1 cut(s) 449
EcoO109I RGGNCCY 1 cut(s) 383
EcoRI GAATTC 2 cut(s) 195, 262
EcoRII CCWGG 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 288
EcoT22I ATGCAT 1 cut(s) 486
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 4 cut(s) 83, 227, 292, 494
FaqI GGGAC 1 cut(s) 396
FatI CATG 4 cut(s) 79, 223, 288, 490
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 1 cut(s) 102
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 1 cut(s) 5
GsuI CTGGAG 1 cut(s) 493
HaeIII GGCC 1 cut(s) 228
HapII CCGG 1 cut(s) 525
HhaI GCGC 1 cut(s) 6
Hin1II CATG 4 cut(s) 83, 227, 292, 494
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HinfI GANTC 3 cut(s) 19, 203, 528
HpaI GTTAAC 1 cut(s) 604
HpaII CCGG 1 cut(s) 525
HphI GGTGA 3 cut(s) 65, 198, 514
Hpy166II GTNNAC 1 cut(s) 604
Hpy188I TCNGA 1 cut(s) 391
Hpy188III TCNNGA 2 cut(s) 179, 200
Hpy8I GTNNAC 1 cut(s) 604
HpyAV CCTTC 1 cut(s) 396
HpyCH4III ACNGT 1 cut(s) 301
HpyCH4IV ACGT 1 cut(s) 612
HpyCH4V TGCA 3 cut(s) 44, 214, 484
HpyF10VI GCNNNNNNNGC 2 cut(s) 330, 475
HpyF3I CTNAG 1 cut(s) 390
HpySE526I ACGT 1 cut(s) 612
Hsp92II CATG 4 cut(s) 83, 227, 292, 494
HspAI GCGC 1 cut(s) 4
KspAI GTTAAC 1 cut(s) 604
Kzo9I GATC 2 cut(s) 171, 587
LweI GCATC 2 cut(s) 81, 85
MaeI CTAG 1 cut(s) 102
MaeII ACGT 1 cut(s) 612
MaeIII GTNAC 5 cut(s) 82, 104, 204, 326, 421
MalI GATC 2 cut(s) 173, 589
MboI GATC 2 cut(s) 171, 587
MboII GAAGA 4 cut(s) 68, 273, 455, 511
MflI RGATCY 1 cut(s) 587
MlsI TGGCCA 1 cut(s) 228
MluCI AATT 4 cut(s) 195, 262, 268, 592
MluNI TGGCCA 1 cut(s) 228
MlyI GAGTC 2 cut(s) 212, 537
MnlI CCTC 4 cut(s) 109, 129, 219, 517
Mox20I TGGCCA 1 cut(s) 228
Mph1103I ATGCAT 1 cut(s) 486
MscI TGGCCA 1 cut(s) 228
MseI TTAA 4 cut(s) 113, 144, 603, 616
MslI CAYNNNNRTG 4 cut(s) 234, 351, 441, 489
Msp20I TGGCCA 1 cut(s) 228
MspA1I CMGCKG 1 cut(s) 469
MspCI CTTAAG 1 cut(s) 143
MspI CCGG 1 cut(s) 525
MspR9I CCNGG 1 cut(s) 251
Mva1269I GAATGC 1 cut(s) 486
MvaI CCWGG 1 cut(s) 251
MwoI GCNNNNNNNGC 2 cut(s) 330, 475
NcoI CCATGG 1 cut(s) 288
NdeII GATC 2 cut(s) 171, 587
NlaIII CATG 4 cut(s) 83, 227, 292, 494
NlaIV GGNNCC 2 cut(s) 384, 418
NmuCI GTSAC 2 cut(s) 204, 326
NsbI TGCGCA 1 cut(s) 5
NsiI ATGCAT 1 cut(s) 486
NspI RCATGY 1 cut(s) 83
NspV TTCGAA 1 cut(s) 193
PctI GAATGC 1 cut(s) 486
PfeI GAWTC 1 cut(s) 19
PleI GAGTC 2 cut(s) 211, 536
PpsI GAGTC 2 cut(s) 211, 536
PpuMI RGGWCCY 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 383
Psp6I CCWGG 1 cut(s) 249
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 2 cut(s) 384, 418
PspPI GGNCC 1 cut(s) 383
PspPPI RGGWCCY 1 cut(s) 383
PstNI CAGNNNCTG 1 cut(s) 330
PsuI RGATCY 1 cut(s) 587
PvuII CAGCTG 1 cut(s) 469
RsaI GTAC 2 cut(s) 69, 158
RsaNI GTAC 2 cut(s) 68, 157
RseI CAYNNNNRTG 4 cut(s) 234, 351, 441, 489
SaqAI TTAA 4 cut(s) 113, 144, 603, 616
Sau3AI GATC 2 cut(s) 171, 587
Sau96I GGNCC 1 cut(s) 383
SchI GAGTC 2 cut(s) 212, 537
ScrFI CCNGG 1 cut(s) 251
SfaNI GCATC 2 cut(s) 81, 85
SfuI TTCGAA 1 cut(s) 193
SinI GGWCC 1 cut(s) 383
SmiMI CAYNNNNRTG 4 cut(s) 234, 351, 441, 489
SmlI CTYRAG 2 cut(s) 120, 143
SmoI CTYRAG 2 cut(s) 120, 143
Sse9I AATT 4 cut(s) 195, 262, 268, 592
SspMI CTAG 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 249
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 1 cut(s) 301
TaiI ACGT 1 cut(s) 615
TaqI TCGA 4 cut(s) 193, 260, 373, 556
TasI AATT 4 cut(s) 195, 262, 268, 592
TfiI GAWTC 1 cut(s) 19
Tru1I TTAA 4 cut(s) 113, 144, 603, 616
Tru9I TTAA 4 cut(s) 113, 144, 603, 616
TscAI CASTG 3 cut(s) 13, 241, 335
TseFI GTSAC 2 cut(s) 204, 326
Tsp45I GTSAC 2 cut(s) 204, 326
TspDTI ATGAA 4 cut(s) 212, 459, 479, 585
TspGWI ACGGA 2 cut(s) 174, 409
TspRI CASTG 3 cut(s) 13, 241, 335
Vha464I CTTAAG 1 cut(s) 143
VpaK11BI GGWCC 1 cut(s) 383
XapI RAATTY 2 cut(s) 195, 262
XceI RCATGY 1 cut(s) 83
XspI CTAG 1 cut(s) 102
Zsp2I ATGCAT 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.