Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0005653

Physical Location & Seq

Genomic Coordinates Reverse (-)

57546960 .. 57552489

Gene Structure 5530 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00123670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2034 bp

ATGCTGAGTTCCGATGGGGGTGAGAAGGATTTGTTCTTTGATTCCGCAGAGTATTTGTCATCTGAAGAGTCAGTGGTAGTGAAGGAGGAGTTGGAGTATGAGATTTGGTTGAATGAGCCACCGAGTGTGAAGGACAGACGAGAGAGTTTTCTACGGCAGATGGGTTTAGCTGAATCTGGATCTACAAAGTTTGGTTCTGTGGAGATGGAAAGGGACGTTGGTACCTCATCGGAGATGATGGGATTGGAGAGACTTTTGGAGTGGAGTGGAGCTGCCTCGAGCTCTTGCAGCTCCTCATCTAGTCACATTGAGGAATATTCGGGTTTTCATAGCAGGGAAGGGGACAATCTAGCAAATTTTATGTATGATGAATTGGACAGAGACCTGCAAGACAAGTCAAATTTAGTTTCCATTCTAGAGTCAGTACAAGTTTCGACTTCTGGTAGTGATTGTTCTCAGGGGGGAACTTGTTCTGAGAGAGAAACTGAGGCTACTGAAGAAGAATGCGAGAGTTCAAAGGAGGGCCGGAAGAAGAAGAGAAGTTGGTGGAAACAGTTTATAAACAAGAGCAAGAGGGGAGGAGGTAAAGTTGTCTCAGAGGTATCAAAACCTGATACTGAAGTATCCAAAGAAAACAGAATGAAGATCAAACAGAACAGTAAGAGGTACATGGAGTTTGCTGCACTTTGCACCGGGCAAGAAATTCAGGCTCACAATGGGTTTATTTGGACGATGAAGTTTAGTCCTGATGGCCAATACATTGCAACTGGTGGTGAAGATGGGGTTGTACGCATTTGGTGTGTTACAGCGGATGCCTCTTGTAATTACCTTATGGCTCAAGGCAACTTAGATAGCAAACTTAAAAAGATCAAGTCTGGATGTCGAGTTATTTTTCCTGACAACAGTTTTCGAATTGAAGAGTCACCTCTCCAAGAGTTTCTTGGCCATTCCAGTGATGTGTTAGACCTGGCTTGGTCAAATTCAAACCGTCTCATTTCTTCGTCCATGGATAAAACTGTTCGTCTGTGGGAAGTTGGTTGCAGTCAGTGCCTACATGTTTTCCATCACAATGACTATGTAACATGCATTCAGTTCAATCCTCTCGATGACAATTACTTCATCAGTGGATCCATAGATGGAAAGGTTCGAGTTTGGGGGCTTTCTGAGAAGCGAGTTGTTGACTGGGCTGATGTGCGAGATGTCATAACAGCTATTTGTTATCAACCAGATGGGAAGGGTTTCGCAGTTGGTTCCCTTGCAGGCACTTGCCGCTTCTATGAAGAATCAGGAAAAGAAGCTCTTCAGCTTGTTGCACAGATTCATATTCGTTCTAGAAGGAAGATCTCTGGCAACAAAATCACCGGCATCCAGTTTTCCGAGGAAAATGCTCAAAGAGTTATGATAACATCAGAAGACTCAAAAGTCCGTGTGTTTGATGGGGTAGATCTCATTAAGAAATACAGAGGTCTTCCAAAGTCAGGAAGTCAAATGTCAGCTTCTTTCACATCCAGCGGGAAACGTATAATCTCAGTTGGAGAGGACTCGCGCATTTATTTATGGGACTATGACAGCTGTAGTGTTCCATCATCCAAGCATACAAAGTCTGTTCGATCCTGCGAACATTTCTATTGCGAAGGTGTGTCTGTTGCAATCCCTTGGTCCGGCATGGGAACAGAAGATAGGAACTTAGAAACTGCTAGTCCACGATATTGCTCACAAACACAAGGCCGAATAGACGCTACTCCTGGCAGTAGAGATTCGGAACGCTTTTTGCTTGGAAATTGGTTCTTTCTGGAGGGTTCCTGCAGAGGCTCTGCAACTTGGCCCGAGGAAAACCTTCCTCAGTGGGATTCACCAGTGGCAGGAGAGGAAGATGATCACTGGCAATACAACGACCACCAACACCATCAGCACCACAATAAAGCTGACAACCACTTGACGAAATCAGAAACGTGGGGGCTTGTCATTGTAACTGCCGGATGGGATGGAAAAATCAGGACATTCCATAACTATGGATTGCCGGTTAGGTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0010008 GO:0012505 GO:0016020 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0048471 GO:0097708 GO:0098588 GO:0098791 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

677

Amino Acids

76.1

Weight (kDa)

5.4

Isoelectric Point (pI)

49.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_CAF1B_HIR1	PF24105	218 - 393	8.3e-16	CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3	PF25174	222 - 269	2.3e-07	THOC3 beta-propeller domain
WD40	PF00400	234 - 266	2.3e-09	WD domain, G-beta repeat
Beta-prop_IFT122_1st	PF23381	292 - 435	2.8e-07	IFT122 first beta-propeller
WD40_CDC20-Fz	PF24807	303 - 410	1.1e-10	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	306 - 386	8.1e-12	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	307 - 539	2.2e-21	Prp19 WD40 domain
WD40	PF00400	308 - 343	2.5e-08	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	309 - 529	8.4e-35	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	309 - 353	1.3e-06	THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	311 - 370	1.6e-08	WDR36/Utp21 second beta-propeller domain
WDR55	PF24796	312 - 412	6.1e-06	WDR55
Beta-prop_THOC3	PF25174	316 - 550	1.4e-25	THOC3 beta-propeller domain
Beta-prop_Aladin	PF25460	318 - 412	6.5e-06	Aladin seven-bladed propeller
Beta-prop_WDR3_2nd	PF25172	319 - 537	3.8e-13	WDR3 second beta-propeller domain
WD40_Gbeta	PF25391	320 - 427	2.8e-10	G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd	PF23393	328 - 446	3.8e-10	WDR90/POC16, second beta-propeller
WD40	PF00400	349 - 385	1.8e-06	WD domain, G-beta repeat
WD40_WDHD1_1st	PF24817	360 - 537	4.4e-09	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	370 - 537	5.5e-15	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	390 - 537	1.1e-08	WDR3 first beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005653)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48870 AT1G48870 AT1G48870
fragaria_vesca	FvH4_1g24010 FvH4_1g24010
malus_domestica	MD01G1011600.v1.1 MD15G1338300.v1.1 MD15G1338400.v1.1
prunus_persica	Prupe.6G159500_v2.0.a1
pyrus_communis	pycom01g04160
rosa_chinensis	RchiOBHm_Chr2g0120151
rosa_laevigata	RLG00000018526 RLG00000020843 RLG00000027801 RLG00000035164
rosa_multiflora	Rmu_co8333869.1_g000001 Rmu_co8364489.1_g000001 Rmu_sc0015469.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123670 Rroxscaffold_7G00185390
rosa_rugosa	Rorug02G0227100.1 Rorug02G0227200
rosa_samantha	Rh2AG283800 Rh2BG295100 Rh2CG272500 Rh2DG308800
rosa_wichuraiana	Rw2G022730 Rw5G023580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	560
AasI	GACNNNNNNGTC	1 cut(s)	1421
Acc36I	ACCTGC	1 cut(s)	393
Acc65I	GGTACC	1 cut(s)	221
AccB1I	GGYRCC	1 cut(s)	221
AccII	CGCG	1 cut(s)	1546
AciI	CCGC	4 cut(s)	45, 809, 1270, 1512
AclWI	GGATC	4 cut(s)	187, 1122, 1135, 1605
AcoI	YGGCCR	2 cut(s)	751, 943
AcsI	RAATTY	4 cut(s)	355, 400, 702, 979
AcuI	CTGAAG	4 cut(s)	84, 516, 639, 1286
AdeI	CACNNNGTG	1 cut(s)	125
AfaI	GTAC	4 cut(s)	223, 426, 668, 789
AfiI	CCNNNNNNNGG	3 cut(s)	1478, 1661, 1862
AflIII	ACRYGT	1 cut(s)	1054
AgsI	TTSAA	5 cut(s)	112, 516, 917, 984, 1096
AjnI	CCWGG	2 cut(s)	966, 1744
AjuI	GAANNNNNNNTTGG	4 cut(s)	74, 106, 201, 233
Alw21I	GWGCWC	1 cut(s)	284
Alw26I	GTCTC	4 cut(s)	244, 375, 598, 995
AlwI	GGATC	4 cut(s)	187, 1122, 1135, 1605
Ama87I	CYCGRG	2 cut(s)	277, 1826
AoxI	GGCC	5 cut(s)	523, 751, 943, 1726, 1823
ApeKI	GCWGC	3 cut(s)	272, 288, 680
ApoI	RAATTY	4 cut(s)	355, 400, 702, 979
Asp700I	GAANNNNTTC	4 cut(s)	469, 1238, 1299, 1836
Asp718I	GGTACC	1 cut(s)	221
AspLEI	GCGC	1 cut(s)	1548
AspS9I	GGNCC	3 cut(s)	523, 1659, 1824
AsuC2I	CCSGG	1 cut(s)	694
AsuHPI	GGTGA	5 cut(s)	32, 785, 915, 1351, 1845
AsuII	TTCGAA	1 cut(s)	910
AvaI	CYCGRG	2 cut(s)	277, 1826
AvaII	GGWCC	1 cut(s)	1659
BaeI	ACNNNNGTAYC	6 cut(s)	205, 238, 606, 606, 639, 639
BalI	TGGCCA	2 cut(s)	753, 945
BamHI	GGATCC	1 cut(s)	1127
BanI	GGYRCC	1 cut(s)	221
BanII	GRGCYC	1 cut(s)	284
BbsI	GAAGAC	2 cut(s)	1419, 1460
Bbv12I	GWGCWC	1 cut(s)	284
BbvI	GCAGC	3 cut(s)	259, 300, 667
BceAI	ACGGC	1 cut(s)	170
BcgI	CGANNNNNNTGC	2 cut(s)	1367, 1401
BciT130I	CCWGG	2 cut(s)	968, 1746
BciVI	GTATCC	1 cut(s)	634
BclI	TGATCA	1 cut(s)	1876
BcnI	CCSGG	1 cut(s)	694
BcoDI	GTCTC	4 cut(s)	244, 375, 598, 995
BfaI	CTAG	5 cut(s)	300, 350, 416, 1332, 1698
BfmI	CTRYAG	2 cut(s)	1573, 1804
BfuAI	ACCTGC	1 cut(s)	393
BfuI	GTATCC	1 cut(s)	634
BglII	AGATCT	2 cut(s)	1341, 1444
BisI	GCNGC	4 cut(s)	273, 289, 681, 1270
BlsI	GCNGC	4 cut(s)	274, 290, 682, 1271
Bme1390I	CCNGG	3 cut(s)	694, 968, 1746
Bme18I	GGWCC	1 cut(s)	1659
BmeT110I	CYCGRG	2 cut(s)	277, 1826
BmgT120I	GGNCC	3 cut(s)	523, 1659, 1824
BmiI	GGNNCC	4 cut(s)	223, 1129, 1252, 1801
BmrFI	CCNGG	3 cut(s)	694, 968, 1746
BmrI	ACTGGG	1 cut(s)	1192
BmsI	GCATC	2 cut(s)	802, 1374
BmuI	ACTGGG	1 cut(s)	1192
BpiI	GAAGAC	2 cut(s)	1419, 1460
BpmI	CTGGAG	1 cut(s)	1814
Bpu14I	TTCGAA	1 cut(s)	910
BpuEI	CTTGAG	1 cut(s)	822
BpuMI	CCSGG	1 cut(s)	694
BsaI	GGTCTC	1 cut(s)	375
BsaJI	CCNNGG	4 cut(s)	1005, 1377, 1655, 1827
BsaXI	ACNNNNNCTCC	4 cut(s)	80, 110, 573, 603
Bsc4I	CCNNNNNNNGG	3 cut(s)	1478, 1661, 1862
Bse118I	RCCGGY	2 cut(s)	1361, 2020
Bse1I	ACTGG	6 cut(s)	772, 951, 1187, 1369, 1856, 1886
Bse3DI	GCAATG	1 cut(s)	759
BseBI	CCWGG	2 cut(s)	968, 1746
BseDI	CCNNGG	4 cut(s)	1005, 1377, 1655, 1827
BseGI	GGATG	7 cut(s)	817, 884, 1365, 1505, 1586, 1985, 1990
BseLI	CCNNNNNNNGG	3 cut(s)	1478, 1661, 1862
BseMI	GCAATG	1 cut(s)	759
BseMII	CTCAG	7 cut(s)	465, 470, 477, 609, 1155, 1542, 1856
BseNI	ACTGG	6 cut(s)	772, 951, 1187, 1369, 1856, 1886
BseRI	GAGGAG	3 cut(s)	101, 283, 594
BseXI	GCAGC	3 cut(s)	259, 300, 667
BsgI	GTGCAG	1 cut(s)	666
Bsh1236I	CGCG	1 cut(s)	1546
BshFI	GGCC	5 cut(s)	525, 753, 945, 1728, 1825
BshNI	GGYRCC	1 cut(s)	221
BsiHKAI	GWGCWC	1 cut(s)	284
BsiHKCI	CYCGRG	2 cut(s)	277, 1826
BsiSI	CCGG	6 cut(s)	526, 693, 1362, 1662, 1977, 2021
BslFI	GGGAC	3 cut(s)	227, 356, 1574
BslI	CCNNNNNNNGG	3 cut(s)	1478, 1661, 1862
BsmAI	GTCTC	4 cut(s)	244, 375, 598, 995
BsmBI	CGTCTC	1 cut(s)	995
BsmFI	GGGAC	3 cut(s)	227, 356, 1574
BsmI	GAATGC	2 cut(s)	509, 1086
BsnI	GGCC	5 cut(s)	525, 753, 945, 1728, 1825
Bso31I	GGTCTC	1 cut(s)	375
BsoBI	CYCGRG	2 cut(s)	277, 1826
Bsp119I	TTCGAA	1 cut(s)	910
Bsp1286I	GDGCHC	1 cut(s)	284
Bsp143I	GATC	8 cut(s)	179, 645, 867, 1127, 1341, 1444, 1610, 1876
Bsp19I	CCATGG	1 cut(s)	1005
BspACI	CCGC	4 cut(s)	45, 809, 1270, 1512
BspANI	GGCC	5 cut(s)	525, 753, 945, 1728, 1825
BspCNI	CTCAG	7 cut(s)	466, 469, 478, 608, 1156, 1541, 1855
BspFNI	CGCG	1 cut(s)	1546
BspLI	GGNNCC	4 cut(s)	223, 1129, 1252, 1801
BspMAI	CTGCAG	1 cut(s)	1808
BspMI	ACCTGC	1 cut(s)	393
BspPI	GGATC	4 cut(s)	187, 1122, 1135, 1605
BspQI	GCTCTTC	1 cut(s)	1305
BspT104I	TTCGAA	1 cut(s)	910
BspT107I	GGYRCC	1 cut(s)	221
BspTNI	GGTCTC	1 cut(s)	375
BsrDI	GCAATG	1 cut(s)	759
BsrFI	RCCGGY	2 cut(s)	1361, 2020
BsrI	ACTGG	6 cut(s)	772, 951, 1187, 1369, 1856, 1886
BssAI	RCCGGY	2 cut(s)	1361, 2020
BssECI	CCNNGG	4 cut(s)	1005, 1377, 1655, 1827
BssMI	GATC	8 cut(s)	179, 645, 867, 1127, 1341, 1444, 1610, 1876
BssT1I	CCWWGG	2 cut(s)	1005, 1655
Bst2UI	CCWGG	2 cut(s)	968, 1746
Bst4CI	ACNGT	5 cut(s)	555, 659, 905, 989, 1018
Bst6I	CTCTTC	4 cut(s)	60, 530, 912, 1305
BstAPI	GCANNNNNTGC	1 cut(s)	1047
BstBI	TTCGAA	1 cut(s)	910
BstC8I	GCNNGC	1 cut(s)	1261
BstDSI	CCRYGG	1 cut(s)	1005
BstF5I	GGATG	7 cut(s)	817, 884, 1365, 1505, 1586, 1985, 1990
BstFNI	CGCG	1 cut(s)	1546
BstHHI	GCGC	1 cut(s)	1548
BstKTI	GATC	8 cut(s)	182, 648, 870, 1130, 1344, 1447, 1613, 1879
BstMAI	GTCTC	4 cut(s)	244, 375, 598, 995
BstMBI	GATC	8 cut(s)	179, 645, 867, 1127, 1341, 1444, 1610, 1876
BstMWI	GCNNNNNNNGC	3 cut(s)	288, 1047, 1269
BstNI	CCWGG	2 cut(s)	968, 1746
BstNSI	RCATGY	2 cut(s)	1058, 1086
BstSCI	CCNGG	3 cut(s)	692, 966, 1744
BstSFI	CTRYAG	2 cut(s)	1573, 1804
BstUI	CGCG	1 cut(s)	1546
BstV1I	GCAGC	3 cut(s)	259, 300, 667
BstV2I	GAAGAC	2 cut(s)	1419, 1460
BstX2I	RGATCY	4 cut(s)	179, 1127, 1341, 1444
BstXI	CCANNNNNNTGG	1 cut(s)	2012
BstYI	RGATCY	4 cut(s)	179, 1127, 1341, 1444
BsuI	GTATCC	1 cut(s)	634
BsuRI	GGCC	5 cut(s)	525, 753, 945, 1728, 1825
BtgI	CCRYGG	1 cut(s)	1005
BtsCI	GGATG	7 cut(s)	817, 884, 1365, 1505, 1586, 1985, 1990
BtsIMutI	CAGTG	7 cut(s)	78, 958, 1052, 1129, 1850, 1863, 1879
BveI	ACCTGC	1 cut(s)	393
Cac8I	GCNNGC	1 cut(s)	1261
CfoI	GCGC	1 cut(s)	1548
Cfr10I	RCCGGY	2 cut(s)	1361, 2020
Cfr13I	GGNCC	3 cut(s)	523, 1659, 1824
CseI	GACGC	1 cut(s)	1745
Csp6I	GTAC	4 cut(s)	222, 425, 667, 788
CviAII	CATG	5 cut(s)	670, 1006, 1055, 1083, 1666
CviQI	GTAC	4 cut(s)	222, 425, 667, 788
DpnI	GATC	8 cut(s)	181, 647, 869, 1129, 1343, 1446, 1612, 1878
DpnII	GATC	8 cut(s)	179, 645, 867, 1127, 1341, 1444, 1610, 1876
DraIII	CACNNNGTG	1 cut(s)	125
DrdI	GACNNNNNNGTC	1 cut(s)	1421
DseDI	GACNNNNNNGTC	1 cut(s)	1421
EaeI	YGGCCR	2 cut(s)	751, 943
Eam1104I	CTCTTC	4 cut(s)	60, 530, 912, 1305
EarI	CTCTTC	4 cut(s)	60, 530, 912, 1305
Ecl136II	GAGCTC	1 cut(s)	282
Eco130I	CCWWGG	2 cut(s)	1005, 1655
Eco24I	GRGCYC	1 cut(s)	284
Eco31I	GGTCTC	1 cut(s)	375
Eco47I	GGWCC	1 cut(s)	1659
Eco53kI	GAGCTC	1 cut(s)	282
Eco57I	CTGAAG	4 cut(s)	84, 516, 639, 1286
Eco88I	CYCGRG	2 cut(s)	277, 1826
EcoICRI	GAGCTC	1 cut(s)	282
EcoRII	CCWGG	2 cut(s)	966, 1744
EcoT14I	CCWWGG	2 cut(s)	1005, 1655
EcoT22I	ATGCAT	1 cut(s)	1088
EcoT38I	GRGCYC	1 cut(s)	284
ErhI	CCWWGG	2 cut(s)	1005, 1655
Esp3I	CGTCTC	1 cut(s)	995
FaeI	CATG	5 cut(s)	673, 1009, 1058, 1086, 1669
FalI	AAGNNNNNCTT	4 cut(s)	924, 956, 1284, 1316
FaqI	GGGAC	3 cut(s)	227, 356, 1574
FatI	CATG	5 cut(s)	669, 1005, 1054, 1082, 1665
FauI	CCCGC	1 cut(s)	1505
FbaI	TGATCA	1 cut(s)	1876
Fnu4HI	GCNGC	4 cut(s)	273, 289, 681, 1270
FokI	GGATG	7 cut(s)	824, 891, 1352, 1492, 1573, 1992, 1997
FriOI	GRGCYC	1 cut(s)	284
Fsp4HI	GCNGC	4 cut(s)	273, 289, 681, 1270
FspBI	CTAG	5 cut(s)	300, 350, 416, 1332, 1698
GlaI	GCGC	1 cut(s)	1547
GluI	GCNGC	4 cut(s)	273, 289, 681, 1270
GsuI	CTGGAG	1 cut(s)	1814
HaeIII	GGCC	5 cut(s)	525, 753, 945, 1728, 1825
HapII	CCGG	6 cut(s)	526, 693, 1362, 1662, 1977, 2021
HgaI	GACGC	1 cut(s)	1745
HhaI	GCGC	1 cut(s)	1548
Hin1II	CATG	5 cut(s)	673, 1009, 1058, 1086, 1669
Hin6I	GCGC	1 cut(s)	1546
HinP1I	GCGC	1 cut(s)	1546
HincII	GTYRAC	1 cut(s)	1180
HindII	GTYRAC	1 cut(s)	1180
HpaII	CCGG	6 cut(s)	526, 693, 1362, 1662, 1977, 2021
HphI	GGTGA	5 cut(s)	32, 785, 915, 1351, 1845
Hpy166II	GTNNAC	2 cut(s)	1180, 1703
Hpy8I	GTNNAC	2 cut(s)	1180, 1703
HpyAV	CCTTC	8 cut(s)	19, 76, 124, 332, 1228, 1329, 1628, 1847
HpyCH4III	ACNGT	5 cut(s)	555, 659, 905, 989, 1018
HpyCH4IV	ACGT	3 cut(s)	216, 1519, 1952
HpyF10VI	GCNNNNNNNGC	3 cut(s)	288, 1047, 1269
HpySE526I	ACGT	3 cut(s)	216, 1519, 1952
Hsp92II	CATG	5 cut(s)	673, 1009, 1058, 1086, 1669
HspAI	GCGC	1 cut(s)	1546
KpnI	GGTACC	1 cut(s)	225
Ksp22I	TGATCA	1 cut(s)	1876
Kzo9I	GATC	8 cut(s)	179, 645, 867, 1127, 1341, 1444, 1610, 1876
LguI	GCTCTTC	1 cut(s)	1305
LmnI	GCTCC	2 cut(s)	269, 296
Lsp1109I	GCAGC	3 cut(s)	259, 300, 667
LweI	GCATC	2 cut(s)	802, 1374
MaeI	CTAG	5 cut(s)	300, 350, 416, 1332, 1698
MaeII	ACGT	3 cut(s)	216, 1519, 1952
MaeIII	GTNAC	5 cut(s)	302, 802, 921, 1078, 1969
MalI	GATC	8 cut(s)	181, 647, 869, 1129, 1343, 1446, 1612, 1878
MboI	GATC	8 cut(s)	179, 645, 867, 1127, 1341, 1444, 1610, 1876
MflI	RGATCY	4 cut(s)	179, 1127, 1341, 1444
MhlI	GDGCHC	1 cut(s)	284
MlsI	TGGCCA	2 cut(s)	753, 945
MluCI	AATT	9 cut(s)	355, 371, 400, 702, 823, 912, 979, 1111, 1780
MluNI	TGGCCA	2 cut(s)	753, 945
MlyI	GAGTC	5 cut(s)	77, 428, 929, 1409, 1535
MmeI	TCCRAC	2 cut(s)	72, 1513
Mox20I	TGGCCA	2 cut(s)	753, 945
Mph1103I	ATGCAT	1 cut(s)	1088
MroXI	GAANNNNTTC	4 cut(s)	469, 1238, 1299, 1836
MscI	TGGCCA	2 cut(s)	753, 945
MseI	TTAA	2 cut(s)	861, 1452
MslI	CAYNNNNRTG	3 cut(s)	951, 1068, 2010
Msp20I	TGGCCA	2 cut(s)	753, 945
MspA1I	CMGCKG	3 cut(s)	809, 1512, 1572
MspI	CCGG	6 cut(s)	526, 693, 1362, 1662, 1977, 2021
MspR9I	CCNGG	3 cut(s)	694, 968, 1746
Mva1269I	GAATGC	2 cut(s)	509, 1086
MvaI	CCWGG	2 cut(s)	968, 1746
MvnI	CGCG	1 cut(s)	1546
MwoI	GCNNNNNNNGC	3 cut(s)	288, 1047, 1269
NciI	CCSGG	1 cut(s)	694
NcoI	CCATGG	1 cut(s)	1005
NdeII	GATC	8 cut(s)	179, 645, 867, 1127, 1341, 1444, 1610, 1876
NlaIII	CATG	5 cut(s)	673, 1009, 1058, 1086, 1669
NlaIV	GGNNCC	4 cut(s)	223, 1129, 1252, 1801
NmuCI	GTSAC	2 cut(s)	302, 921
NsiI	ATGCAT	1 cut(s)	1088
NspI	RCATGY	2 cut(s)	1058, 1086
NspV	TTCGAA	1 cut(s)	910
PaeR7I	CTCGAG	1 cut(s)	277
PciI	ACATGT	1 cut(s)	1054
PciSI	GCTCTTC	1 cut(s)	1305
PctI	GAATGC	2 cut(s)	509, 1086
PdmI	GAANNNNTTC	4 cut(s)	469, 1238, 1299, 1836
PfeI	GAWTC	6 cut(s)	41, 173, 1283, 1318, 1757, 1850
PkrI	GCNGC	4 cut(s)	274, 290, 682, 1271
PleI	GAGTC	5 cut(s)	76, 427, 928, 1409, 1535
PpsI	GAGTC	5 cut(s)	76, 427, 928, 1409, 1535
PscI	ACATGT	1 cut(s)	1054
PsiI	TTATAA	1 cut(s)	560
Psp124BI	GAGCTC	1 cut(s)	284
Psp6I	CCWGG	2 cut(s)	966, 1744
PspGI	CCWGG	2 cut(s)	966, 1744
PspN4I	GGNNCC	4 cut(s)	223, 1129, 1252, 1801
PspPI	GGNCC	3 cut(s)	523, 1659, 1824
PspXI	VCTCGAGB	1 cut(s)	277
PsrI	GAACNNNNNNTAC	2 cut(s)	436, 468
PstI	CTGCAG	1 cut(s)	1808
PsuI	RGATCY	4 cut(s)	179, 1127, 1341, 1444
PvuII	CAGCTG	1 cut(s)	1572
RsaI	GTAC	4 cut(s)	223, 426, 668, 789
RsaNI	GTAC	4 cut(s)	222, 425, 667, 788
RseI	CAYNNNNRTG	3 cut(s)	951, 1068, 2010
SacI	GAGCTC	1 cut(s)	284
SapI	GCTCTTC	1 cut(s)	1305
SaqAI	TTAA	2 cut(s)	861, 1452
SatI	GCNGC	4 cut(s)	273, 289, 681, 1270
Sau3AI	GATC	8 cut(s)	179, 645, 867, 1127, 1341, 1444, 1610, 1876
Sau96I	GGNCC	3 cut(s)	523, 1659, 1824
SchI	GAGTC	5 cut(s)	77, 428, 929, 1409, 1535
ScrFI	CCNGG	3 cut(s)	694, 968, 1746
SduI	GDGCHC	1 cut(s)	284
SfaNI	GCATC	2 cut(s)	802, 1374
SfcI	CTRYAG	2 cut(s)	1573, 1804
Sfr274I	CTCGAG	1 cut(s)	277
SfuI	TTCGAA	1 cut(s)	910
SinI	GGWCC	1 cut(s)	1659
SlaI	CTCGAG	1 cut(s)	277
SmiMI	CAYNNNNRTG	3 cut(s)	951, 1068, 2010
SmlI	CTYRAG	2 cut(s)	277, 837
SmoI	CTYRAG	2 cut(s)	277, 837
Sse9I	AATT	9 cut(s)	355, 371, 400, 702, 823, 912, 979, 1111, 1780
SsiI	CCGC	4 cut(s)	45, 809, 1270, 1512
SspI	AATATT	1 cut(s)	317
SspMI	CTAG	5 cut(s)	300, 350, 416, 1332, 1698
SstI	GAGCTC	1 cut(s)	284
StyD4I	CCNGG	3 cut(s)	692, 966, 1744
StyI	CCWWGG	2 cut(s)	1005, 1655
TaaI	ACNGT	5 cut(s)	555, 659, 905, 989, 1018
TaiI	ACGT	3 cut(s)	219, 1522, 1955
TaqI	TCGA	7 cut(s)	278, 434, 883, 910, 1104, 1147, 1609
TasI	AATT	9 cut(s)	355, 371, 400, 702, 823, 912, 979, 1111, 1780
TatI	WGTACW	1 cut(s)	424
TauI	GCSGC	1 cut(s)	1272
TfiI	GAWTC	6 cut(s)	41, 173, 1283, 1318, 1757, 1850
Tru1I	TTAA	2 cut(s)	861, 1452
Tru9I	TTAA	2 cut(s)	861, 1452
TscAI	CASTG	7 cut(s)	78, 958, 1052, 1129, 1850, 1863, 1886
TseFI	GTSAC	2 cut(s)	302, 921
TseI	GCWGC	3 cut(s)	272, 288, 680
Tsp45I	GTSAC	2 cut(s)	302, 921
TspDTI	ATGAA	7 cut(s)	317, 384, 656, 749, 1108, 1293, 1310
TspGWI	ACGGA	1 cut(s)	1415
TspRI	CASTG	7 cut(s)	78, 958, 1052, 1129, 1850, 1863, 1886
VpaK11BI	GGWCC	1 cut(s)	1659
XapI	RAATTY	4 cut(s)	355, 400, 702, 979
XbaI	TCTAGA	2 cut(s)	415, 1331
XceI	RCATGY	2 cut(s)	1058, 1086
XcmI	CCANNNNNNNNNTGG	1 cut(s)	938
XhoI	CTCGAG	1 cut(s)	277
XmnI	GAANNNNTTC	4 cut(s)	469, 1238, 1299, 1836
XspI	CTAG	5 cut(s)	300, 350, 416, 1332, 1698
Zsp2I	ATGCAT	1 cut(s)	1088

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.