RLG00000035164

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0005653
Physical Location & Seq
Reverse (-)
62057665 .. 62061315
3651 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035164

Sequence Viewer

Length: 822 bp
ATGGGATGGTTTGCACAGTGGTCAGTTGAAGAAGAACATGAAGAAAGATTCCCACCATTTACGTCTCTTTTTTCTTTGGATTTCCTCCCTCCTCCTGCTACTGCTTTATTCATTTCTCAGATCGCCGAACCAATCGACCCGAATCGAAATCCGGGTGTAATCTTTTCTGGGACGGACATCCGAGGAGAAGAAGTATCTGATATTGAAAGCTTCGATCCATATCAGGAATTGTTGAAAGCTTTGATCGGTTTCATAGATGTTGCTGGCCTTTACTTTGCTTTGACCCAGTTGACCCATAGGAACATCTGTCAAAATCATAAATTGCAAGCGCTATTGCTTTCATCTTCAATGGACAAAACTGTTAGATTATGGGATATGGCGACCAAGAGTTGTTTAAAAATGCTTGCCCATAATGATTATGTAACTTGCATACAGTTCAATCCTTTGGACGACAGTGTTTTTCTCAGCGGCTCCCTTGATGCGAAGTTTCGATTATGGGACATACCTGATCTAAGAATGGTAGACTGGACTGATCTTCATGAAATGGTCATTGCTGCATGCTATCCTCCAGAAGGCCAATTAACAGTTGACTTTGGTGGAAAAATAGTGCGCCAAAGTGATTTACGCCGTCGCCGGCCTCCATCAGGATGCTTCAGCAAGCCACTGATAATTTCTTCAAGATTCGCTCCACCAGAACTTGCGAATTGGTTCCTCCCTCCTTTGCGTCGGCTCTCGGATTTGAATGGAAAATTGAGGTGCCATTGCAAAAGTGAAGATCATATTGAATTGGACAAGACCCACACTGCGCATATAGCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

274

Amino Acids

31.09

Weight (kDa)

5.46

Isoelectric Point (pI)

59.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2053 PF09767 66 - 113 4.8e-07 Predicted membrane protein (DUF2053)
WD40_Prp19 PF24814 104 - 193 1.5e-08 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 106 - 192 8.2e-16 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 108 - 168 2.5e-10 WDR3 first beta-propeller domain
Beta-prop_Aladin PF25460 109 - 194 2.7e-08 Aladin seven-bladed propeller
Beta-prop_WDR3_2nd PF25172 110 - 181 2e-06 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 110 - 194 8e-06 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 111 - 194 1.6e-12 THOC3 beta-propeller domain
Beta-prop_WDR75_1st PF23869 111 - 174 4.8e-06 WD repeat-containing protein 75 first beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 807
AccB1I GGYRCC 1 cut(s) 756
AccI GTMKAC 1 cut(s) 522
AciI CCGC 1 cut(s) 468
AclWI GGATC 1 cut(s) 209
AcuI CTGAAG 1 cut(s) 637
AfeI AGCGCT 1 cut(s) 330
AfiI CCNNNNNNNGG 2 cut(s) 572, 644
AgsI TTSAA 8 cut(s) 29, 206, 235, 348, 439, 678, 742, 785
AluBI AGCT 3 cut(s) 210, 239, 815
AluI AGCT 3 cut(s) 210, 239, 815
Alw26I GTCTC 1 cut(s) 69
AlwI GGATC 1 cut(s) 209
Aor51HI AGCGCT 1 cut(s) 330
AoxI GGCC 3 cut(s) 265, 574, 635
ApeKI GCWGC 1 cut(s) 554
Asp700I GAANNNNTTC 1 cut(s) 707
AspLEI GCGC 3 cut(s) 331, 612, 808
AsuC2I CCSGG 1 cut(s) 153
BanI GGYRCC 1 cut(s) 756
BbvI GCAGC 1 cut(s) 541
BccI CCATC 1 cut(s) 649
BceAI ACGGC 1 cut(s) 612
BcnI CCSGG 1 cut(s) 153
BcoDI GTCTC 1 cut(s) 69
BfoI RGCGCY 1 cut(s) 332
BisI GCNGC 2 cut(s) 469, 555
BlsI GCNGC 2 cut(s) 470, 556
Bme1390I CCNGG 1 cut(s) 153
BmiI GGNNCC 3 cut(s) 472, 710, 758
BmrFI CCNGG 1 cut(s) 153
BmrI ACTGGG 1 cut(s) 280
BmsI GCATC 2 cut(s) 469, 638
BmuI ACTGGG 1 cut(s) 280
BpmI CTGGAG 1 cut(s) 552
BpuMI CCSGG 1 cut(s) 153
BsaBI GATNNNNATC 1 cut(s) 219
BsaJI CCNNGG 1 cut(s) 181
BsaXI ACNNNNNCTCC 2 cut(s) 177, 207
Bsc4I CCNNNNNNNGG 2 cut(s) 572, 644
Bse118I RCCGGY 1 cut(s) 633
Bse1I ACTGG 2 cut(s) 286, 530
Bse3DI GCAATG 2 cut(s) 549, 760
Bse8I GATNNNNATC 1 cut(s) 219
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 3 cut(s) 11, 177, 653
BseJI GATNNNNATC 1 cut(s) 219
BseLI CCNNNNNNNGG 2 cut(s) 572, 644
BseMI GCAATG 2 cut(s) 549, 760
BseMII CTCAG 2 cut(s) 131, 478
BseNI ACTGG 2 cut(s) 286, 530
BseRI GAGGAG 2 cut(s) 81, 198
BseXI GCAGC 1 cut(s) 541
BshFI GGCC 3 cut(s) 267, 576, 637
BshNI GGYRCC 1 cut(s) 756
BsiSI CCGG 2 cut(s) 152, 634
BslFI GGGAC 2 cut(s) 184, 512
BslI CCNNNNNNNGG 2 cut(s) 572, 644
BsmAI GTCTC 1 cut(s) 69
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 2 cut(s) 184, 512
BsnI GGCC 3 cut(s) 267, 576, 637
Bsp143I GATC 6 cut(s) 120, 214, 243, 508, 532, 775
BspACI CCGC 1 cut(s) 468
BspANI GGCC 3 cut(s) 267, 576, 637
BspCNI CTCAG 2 cut(s) 130, 477
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 3 cut(s) 472, 710, 758
BspPI GGATC 1 cut(s) 209
BspT107I GGYRCC 1 cut(s) 756
BsrDI GCAATG 2 cut(s) 549, 760
BsrFI RCCGGY 1 cut(s) 633
BsrI ACTGG 2 cut(s) 286, 530
BssAI RCCGGY 1 cut(s) 633
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 6 cut(s) 120, 214, 243, 508, 532, 775
Bst4CI ACNGT 5 cut(s) 18, 361, 435, 455, 586
BstC8I GCNNGC 6 cut(s) 265, 327, 405, 559, 635, 659
BstDEI CTNAG 3 cut(s) 117, 464, 512
BstENI CCTNNNNNAGG 2 cut(s) 570, 642
BstF5I GGATG 3 cut(s) 11, 177, 653
BstH2I RGCGCY 1 cut(s) 332
BstHHI GCGC 3 cut(s) 331, 612, 808
BstKTI GATC 6 cut(s) 123, 217, 246, 511, 535, 778
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 6 cut(s) 120, 214, 243, 508, 532, 775
BstMWI GCNNNNNNNGC 1 cut(s) 812
BstNSI RCATGY 1 cut(s) 561
BstSCI CCNGG 1 cut(s) 151
BstV1I GCAGC 1 cut(s) 541
BsuRI GGCC 3 cut(s) 267, 576, 637
BtsCI GGATG 3 cut(s) 11, 177, 653
BtsI GCAGTG 1 cut(s) 801
BtsIMutI CAGTG 4 cut(s) 23, 460, 662, 801
Cac8I GCNNGC 6 cut(s) 265, 327, 405, 559, 635, 659
CciI TCATGA 1 cut(s) 538
CfoI GCGC 3 cut(s) 331, 612, 808
Cfr10I RCCGGY 1 cut(s) 633
CseI GACGC 1 cut(s) 713
CviAII CATG 3 cut(s) 38, 539, 558
CviJI RGCY 9 cut(s) 210, 239, 267, 471, 576, 637, 661, 730, 815
CviKI_1 RGCY 9 cut(s) 210, 239, 267, 471, 576, 637, 661, 730, 815
DdeI CTNAG 3 cut(s) 117, 464, 512
DpnI GATC 6 cut(s) 122, 216, 245, 510, 534, 777
DpnII GATC 6 cut(s) 120, 214, 243, 508, 532, 775
DraI TTTAAA 1 cut(s) 396
Eco47III AGCGCT 1 cut(s) 330
Eco57I CTGAAG 1 cut(s) 637
EcoNI CCTNNNNNAGG 2 cut(s) 570, 642
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 3 cut(s) 41, 542, 561
FaqI GGGAC 2 cut(s) 184, 512
FatI CATG 3 cut(s) 37, 538, 557
FblI GTMKAC 1 cut(s) 522
Fnu4HI GCNGC 2 cut(s) 469, 555
FokI GGATG 3 cut(s) 18, 164, 660
Fsp4HI GCNGC 2 cut(s) 469, 555
FspI TGCGCA 1 cut(s) 807
GlaI GCGC 3 cut(s) 330, 611, 807
GluI GCNGC 2 cut(s) 469, 555
GsuI CTGGAG 1 cut(s) 552
HaeII RGCGCY 1 cut(s) 332
HaeIII GGCC 3 cut(s) 267, 576, 637
HapII CCGG 2 cut(s) 152, 634
HgaI GACGC 1 cut(s) 713
HhaI GCGC 3 cut(s) 331, 612, 808
Hin1II CATG 3 cut(s) 41, 542, 561
Hin6I GCGC 3 cut(s) 329, 610, 806
HinP1I GCGC 3 cut(s) 329, 610, 806
HincII GTYRAC 2 cut(s) 291, 589
HindII GTYRAC 2 cut(s) 291, 589
HindIII AAGCTT 2 cut(s) 208, 237
HinfI GANTC 3 cut(s) 48, 142, 681
HpaII CCGG 2 cut(s) 152, 634
Hpy166II GTNNAC 3 cut(s) 291, 523, 589
Hpy188I TCNGA 4 cut(s) 120, 182, 199, 736
Hpy188III TCNNGA 5 cut(s) 224, 539, 569, 645, 678
Hpy8I GTNNAC 3 cut(s) 291, 523, 589
Hpy99I CGWCG 2 cut(s) 633, 729
HpyAV CCTTC 1 cut(s) 566
HpyCH4III ACNGT 5 cut(s) 18, 361, 435, 455, 586
HpyCH4IV ACGT 1 cut(s) 62
HpyCH4V TGCA 5 cut(s) 14, 325, 429, 557, 765
HpyF10VI GCNNNNNNNGC 1 cut(s) 812
HpyF3I CTNAG 3 cut(s) 117, 464, 512
HpySE526I ACGT 1 cut(s) 62
Hsp92II CATG 3 cut(s) 41, 542, 561
HspAI GCGC 3 cut(s) 329, 610, 806
KroI GCCGGC 1 cut(s) 633
KroNI GCCGGC 1 cut(s) 635
Kzo9I GATC 6 cut(s) 120, 214, 243, 508, 532, 775
LmnI GCTCC 2 cut(s) 476, 691
Lsp1109I GCAGC 1 cut(s) 541
LweI GCATC 2 cut(s) 469, 638
MaeII ACGT 1 cut(s) 62
MaeIII GTNAC 1 cut(s) 421
MalI GATC 6 cut(s) 122, 216, 245, 510, 534, 777
MboI GATC 6 cut(s) 120, 214, 243, 508, 532, 775
MboII GAAGA 8 cut(s) 41, 44, 53, 200, 336, 527, 666, 785
MluCI AATT 7 cut(s) 227, 320, 578, 669, 703, 749, 785
MnlI CCTC 9 cut(s) 95, 99, 102, 176, 576, 648, 722, 726, 747
MroNI GCCGGC 1 cut(s) 633
MroXI GAANNNNTTC 1 cut(s) 707
MseI TTAA 2 cut(s) 395, 581
MslI CAYNNNNRTG 1 cut(s) 646
MspA1I CMGCKG 1 cut(s) 468
MspI CCGG 2 cut(s) 152, 634
MspR9I CCNGG 1 cut(s) 153
MwoI GCNNNNNNNGC 1 cut(s) 812
NaeI GCCGGC 1 cut(s) 635
NciI CCSGG 1 cut(s) 153
NdeII GATC 6 cut(s) 120, 214, 243, 508, 532, 775
NgoMIV GCCGGC 1 cut(s) 633
NlaIII CATG 3 cut(s) 41, 542, 561
NlaIV GGNNCC 3 cut(s) 472, 710, 758
NsbI TGCGCA 1 cut(s) 807
NspI RCATGY 1 cut(s) 561
PaeI GCATGC 1 cut(s) 561
PagI TCATGA 1 cut(s) 538
PdiI GCCGGC 1 cut(s) 635
PdmI GAANNNNTTC 1 cut(s) 707
PfeI GAWTC 3 cut(s) 48, 142, 681
PkrI GCNGC 2 cut(s) 470, 556
PspN4I GGNNCC 3 cut(s) 472, 710, 758
RseI CAYNNNNRTG 1 cut(s) 646
SaqAI TTAA 2 cut(s) 395, 581
SatI GCNGC 2 cut(s) 469, 555
Sau3AI GATC 6 cut(s) 120, 214, 243, 508, 532, 775
ScrFI CCNGG 1 cut(s) 153
SetI ASST 6 cut(s) 65, 212, 241, 508, 758, 817
SfaNI GCATC 2 cut(s) 469, 638
SmiMI CAYNNNNRTG 1 cut(s) 646
SphI GCATGC 1 cut(s) 561
Sse9I AATT 7 cut(s) 227, 320, 578, 669, 703, 749, 785
SsiI CCGC 1 cut(s) 468
StyD4I CCNGG 1 cut(s) 151
TaaI ACNGT 5 cut(s) 18, 361, 435, 455, 586
TaiI ACGT 1 cut(s) 65
TaqI TCGA 4 cut(s) 135, 145, 213, 490
TasI AATT 7 cut(s) 227, 320, 578, 669, 703, 749, 785
TauI GCSGC 1 cut(s) 471
TfiI GAWTC 3 cut(s) 48, 142, 681
Tru1I TTAA 2 cut(s) 395, 581
Tru9I TTAA 2 cut(s) 395, 581
TscAI CASTG 4 cut(s) 23, 460, 669, 808
TseI GCWGC 1 cut(s) 554
TspDTI ATGAA 6 cut(s) 54, 100, 241, 330, 527, 555
TspGWI ACGGA 1 cut(s) 188
TspRI CASTG 4 cut(s) 23, 460, 669, 808
XagI CCTNNNNNAGG 2 cut(s) 570, 642
XceI RCATGY 1 cut(s) 561
XmiI GTMKAC 1 cut(s) 522
XmnI GAANNNNTTC 1 cut(s) 707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.