Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0005653

Physical Location & Seq

Genomic Coordinates Forward (+)

6192437 .. 6205819

Gene Structure 13383 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1011600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1941 bp

ATGCTGAGCTCTGATCAATCTTATGATGAAGAAAATGATGTGTTCTTCGACTCGGAGGACTTGTCACCTACACGATCACTCATAGCGAGTGAGGAGTACAAAATTTGGATGAATGAGCCACCAAGTGTTAAGGAGAGACGGGAGAGTTTTCTGCGAAAAATGGATTTAGTTGAGTTTGATTCTGCAAAGTTTGCTTCTGTGGATATGGGATTGGAAAGAGTTTCGGAATGCAGTGGAGCTTCCCCAAGCTCTTGCATTTCGTCTTCTAGTCGTCATCAAGCAAACTTTATGTATAATACATTGGATAGTAACATACAAGGTAGCTCAAATTTGGCCTGGACTCACGATGCAGTTCATATTGCTTCACCTTCTGCTACGGGTTCTGCTCGGAGAGAAGCTGAGGCTCCAGAAAAAGAATGTTGGAATTCACAGGAGGGTGAAAAGAAGACGAAAGTTTGGTGGAAACAGTTTATAAACAAGAGGAAGAGAAGAGGAGGTAAAGTTGTGTCAGAGGTATCGAAACCAGATACTGAAATACCGAAACAAAATAGGATGATGGTCAAGCAGAATGGTAAGAAGTGCATGGAGTTCACTGCACTATGCAGCGGGCAAGAAATTCATGCGCACAAGGGGTTCATTGGGGTGATGAAGTTTAGTCTCGATGGCCAGTATCTCGCAACTGGCGGTGAAGATGGGATTGTATGCATCTGGCGTGTTACATTAACAGATGTTTCTTGTACCTACTTAACACCTCAAGGGAACTTTGATGGCAAACTTAAGAAAATAAAGTCCGGTTATCGCGTGATCTTTCCTGAAAAGGGTTTTCGAATTCAGGAGTCACCGCTGCAAGAGTTTCATGGCCATTCCAGTGATGTGTTAGACCTGGCTTGGTCGAGTTCAAATTATCTTCTTTCTTCGTCCATGGATAAAACTGTTCGTCTGTGGGATGTTAGTTGCGGTGACTGCCTAAATGTTTTCGATCACAATGACTATGTGACGTGCATTCAGTTCAATCCTGTCAACAACAATTATTTCATAAGTGGATCGATAGATGGGAAAGTTCGAGTTTGGGGACTCTCTGATAACCGAGTTGCTGACTGGGCTGATGTTCGAGATGTTATAACTGCTATATGTTATCAACCAGATGGAAAAGGTTTCGTGGTTGGTTCCGTTACAGGCACTTGCCACTTCTATGAAGCATCAGGGGACTATCTTCAGCTGGTTGTGCGAATGCGTATTCATGGTAGGAAGAAAACCTCTGGCAACCAAATCACTGGCATCAAGTTTTGCCAGGAAAAGTCGCAGAGAGTTATGATAACATCAGAAGACTCGAAAGTCCGTATATTTGACGGAGTAGAACTTACAAGGAAATACAAAGGTCTTGCAAAGTCGGGAAGTCAGACGTCAGCTTCTTTTACATCGAGCGGGAAACATATAATTTCAGTTGGAGAGGACTCACGTGTTTATTTGTGGGACTATGACGAGCCGTGTGTCTCATCATCATCCAAACAAACGAAATCTGTTCAATCCTGTGAGCATTTCTTATGCGAAGGTGTGTCTGTCGCGGTACCTTGGCCAGGCATGAGAAGAGAACAAAGGAGCTTGGAAACACCAGGCCATCTGGAGGCTGCTCCAGGAAGCAGAGATTCAGAATGTTTTTCTCTTGGAAATTGGTTTTTCTTAAATGGTCCGTGCAGGGGTGGCTCTGCAACTTGGCCCGAAGAGAAACTTCCTCTGCCTTTATGGGATTTACCGGTCATACGAGATGAAAATGGTCAGTGTCACTGGCAACGTCAAGTTCACCATCGTCAGCAGCAGCAACACAATAGAGCACTCAATCACACCGCTGAATCAGAACCATGGGGGCTTGTCATTGCAACAGCTGGATGTGACGGAACAATCAGGACATTCCATAACTACGGATTGCCAGTCAGATTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005829 GO:0010008 GO:0012505 GO:0016020 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0048471 GO:0097708 GO:0098588 GO:0098791 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

647

Amino Acids

72.57

Weight (kDa)

6.56

Isoelectric Point (pI)

51.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	185 - 240	4.8e-06	THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	205 - 364	1.2e-16	CAF1B/HIR1 beta-propeller domain
WD40_Gbeta	PF25391	210 - 456	1.6e-11	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	276 - 391	3.6e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	278 - 358	8.3e-13	WDR3 first beta-propeller domain
WD40_Prp19	PF24814	279 - 401	2.4e-14	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	280 - 325	2e-07	THOC3 beta-propeller domain
WD40	PF00400	280 - 316	1.8e-09	WD domain, G-beta repeat
Beta-prop_WDR5	PF25175	281 - 504	1e-34	WDR5 beta-propeller domain
WDR55	PF24796	282 - 450	4.8e-09	WDR55
Beta-prop_WDR35_TULP_N	PF24797	283 - 438	4.8e-06	WDR35/TULP4 N-terminal
EIF3I	PF24805	283 - 483	1.7e-09	EIF3I
Beta-prop_WDR36-Utp21_2nd	PF25168	283 - 342	5e-09	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	284 - 357	1.5e-09	WDR3 second beta-propeller domain
Beta-prop_Aladin	PF25460	288 - 388	3.1e-07	Aladin seven-bladed propeller
Beta-prop_THOC3	PF25174	288 - 518	1.5e-26	THOC3 beta-propeller domain
Beta-prop_WDR90_POC16_2nd	PF23393	303 - 408	2.4e-09	WDR90/POC16, second beta-propeller
WD40	PF00400	320 - 357	2.9e-06	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	341 - 528	8.8e-15	Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st	PF24817	341 - 523	2.9e-09	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	362 - 511	3.1e-09	WDR3 first beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0005653)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48870 AT1G48870 AT1G48870
fragaria_vesca	FvH4_1g24010 FvH4_1g24010
malus_domestica	MD01G1011600.v1.1 MD15G1338300.v1.1 MD15G1338400.v1.1
prunus_persica	Prupe.6G159500_v2.0.a1
pyrus_communis	pycom01g04160
rosa_chinensis	RchiOBHm_Chr2g0120151
rosa_laevigata	RLG00000018526 RLG00000020843 RLG00000027801 RLG00000035164
rosa_multiflora	Rmu_co8333869.1_g000001 Rmu_co8364489.1_g000001 Rmu_sc0015469.1_g000001
rosa_roxburghii	Rroxscaffold_2G00123670 Rroxscaffold_7G00185390
rosa_rugosa	Rorug02G0227100.1 Rorug02G0227200
rosa_samantha	Rh2AG283800 Rh2BG295100 Rh2CG272500 Rh2DG308800
rosa_wichuraiana	Rw2G022730 Rw5G023580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	473, 1121
AasI	GACNNNNNNGTC	1 cut(s)	1334
AatII	GACGTC	1 cut(s)	1406
Acc16I	TGCGCA	1 cut(s)	624
Acc65I	GGTACC	1 cut(s)	1567
AccB1I	GGYRCC	1 cut(s)	1567
AccBSI	CCGCTC	1 cut(s)	1425
AccII	CGCG	2 cut(s)	801, 1565
AciI	CCGC	7 cut(s)	606, 684, 842, 957, 1425, 1565, 1845
AclWI	GGATC	1 cut(s)	1051
AcoI	YGGCCR	3 cut(s)	664, 859, 1574
AcsI	RAATTY	5 cut(s)	102, 328, 424, 615, 828
AcuI	CTGAAG	1 cut(s)	1199
AcvI	CACGTG	1 cut(s)	1460
AcyI	GRCGYC	1 cut(s)	1403
AdeI	CACNNNGTG	1 cut(s)	125
AfaI	GTAC	3 cut(s)	98, 739, 1569
AfiI	CCNNNNNNNGG	4 cut(s)	818, 1577, 1624, 1697
AflII	CTTAAG	1 cut(s)	776
AflIII	ACRYGT	1 cut(s)	1459
AgeI	ACCGGT	1 cut(s)	1753
AgsI	TTSAA	3 cut(s)	900, 1012, 1526
AjiI	CACGTC	1 cut(s)	999
AjnI	CCWGG	6 cut(s)	335, 882, 1290, 1576, 1612, 1633
AluBI	AGCT	9 cut(s)	9, 239, 249, 324, 398, 1219, 1409, 1602, 1883
AluI	AGCT	9 cut(s)	9, 239, 249, 324, 398, 1219, 1409, 1602, 1883
Alw21I	GWGCWC	2 cut(s)	11, 1834
Alw26I	GTCTC	3 cut(s)	130, 662, 1498
AlwI	GGATC	1 cut(s)	1051
AlwNI	CAGNNNCTG	1 cut(s)	530
AoxI	GGCC	6 cut(s)	333, 664, 859, 1574, 1615, 1715
ApeKI	GCWGC	5 cut(s)	603, 844, 1628, 1813, 1816
ApoI	RAATTY	5 cut(s)	102, 328, 424, 615, 828
AsiGI	ACCGGT	1 cut(s)	1753
Asp718I	GGTACC	1 cut(s)	1567
AspLEI	GCGC	1 cut(s)	625
AspS9I	GGNCC	2 cut(s)	1688, 1716
AsuHPI	GGTGA	8 cut(s)	57, 357, 449, 655, 698, 831, 971, 1793
AsuII	TTCGAA	1 cut(s)	826
AvaII	GGWCC	1 cut(s)	1688
BalI	TGGCCA	3 cut(s)	666, 861, 1576
BanI	GGYRCC	1 cut(s)	1567
BanII	GRGCYC	1 cut(s)	11
BbrPI	CACGTG	1 cut(s)	1460
BbsI	GAAGAC	3 cut(s)	255, 452, 1332
Bbv12I	GWGCWC	2 cut(s)	11, 1834
BbvCI	CCTCAGC	1 cut(s)	399
BbvI	GCAGC	5 cut(s)	615, 831, 1615, 1825, 1828
BccI	CCATC	8 cut(s)	550, 656, 686, 761, 1046, 1139, 1626, 1812
BceAI	ACGGC	1 cut(s)	1471
BciT130I	CCWGG	6 cut(s)	337, 884, 1292, 1578, 1614, 1635
BclI	TGATCA	1 cut(s)	13
BcoDI	GTCTC	3 cut(s)	130, 662, 1498
BfaI	CTAG	1 cut(s)	267
BfrI	CTTAAG	1 cut(s)	776
BisI	GCNGC	5 cut(s)	604, 845, 1629, 1814, 1817
BlpI	GCTNAGC	1 cut(s)	5
BlsI	GCNGC	5 cut(s)	605, 846, 1630, 1815, 1818
Bme1390I	CCNGG	6 cut(s)	337, 884, 1292, 1578, 1614, 1635
Bme18I	GGWCC	1 cut(s)	1688
BmgBI	CACGTC	1 cut(s)	999
BmgT120I	GGNCC	2 cut(s)	1688, 1716
BmiI	GGNNCC	3 cut(s)	405, 1168, 1569
BmrFI	CCNGG	6 cut(s)	337, 884, 1292, 1578, 1614, 1635
BmrI	ACTGGG	1 cut(s)	1108
BmsI	GCATC	4 cut(s)	337, 714, 1208, 1287
BmuI	ACTGGG	1 cut(s)	1108
BpiI	GAAGAC	3 cut(s)	255, 452, 1332
BpmI	CTGGAG	3 cut(s)	390, 1617, 1643
Bpu10I	CCTNAGC	1 cut(s)	399
Bpu1102I	GCTNAGC	1 cut(s)	5
Bpu14I	TTCGAA	1 cut(s)	826
BpuEI	CTTGAG	1 cut(s)	738
Bsa29I	ATCGAT	1 cut(s)	1046
BsaAI	YACGTR	1 cut(s)	1460
BsaHI	GRCGYC	1 cut(s)	1403
BsaJI	CCNNGG	3 cut(s)	921, 1571, 1859
BsaWI	WCCGGW	2 cut(s)	791, 1753
BsaXI	ACNNNNNCTCC	4 cut(s)	47, 77, 486, 516
Bsc4I	CCNNNNNNNGG	4 cut(s)	818, 1577, 1624, 1697
Bse118I	RCCGGY	1 cut(s)	1753
Bse1I	ACTGG	7 cut(s)	667, 685, 867, 1103, 1279, 1790, 1928
Bse3DI	GCAATG	1 cut(s)	1872
BseBI	CCWGG	6 cut(s)	337, 884, 1292, 1578, 1614, 1635
BseCI	ATCGAT	1 cut(s)	1046
BseDI	CCNNGG	3 cut(s)	921, 1571, 1859
BseGI	GGATG	5 cut(s)	114, 558, 952, 1502, 1892
BseLI	CCNNNNNNNGG	4 cut(s)	818, 1577, 1624, 1697
BseMI	GCAATG	1 cut(s)	1872
BseMII	CTCAG	1 cut(s)	390
BseNI	ACTGG	7 cut(s)	667, 685, 867, 1103, 1279, 1790, 1928
BseRI	GAGGAG	2 cut(s)	107, 507
BseXI	GCAGC	5 cut(s)	615, 831, 1615, 1825, 1828
BsgI	GTGCAG	2 cut(s)	579, 1714
Bsh1236I	CGCG	2 cut(s)	801, 1565
BshFI	GGCC	6 cut(s)	335, 666, 861, 1576, 1617, 1717
BshNI	GGYRCC	1 cut(s)	1567
BshTI	ACCGGT	1 cut(s)	1753
BshVI	ATCGAT	1 cut(s)	1046
BsiHKAI	GWGCWC	2 cut(s)	11, 1834
BsiSI	CCGG	2 cut(s)	792, 1754
BslFI	GGGAC	3 cut(s)	1086, 1220, 1487
BslI	CCNNNNNNNGG	4 cut(s)	818, 1577, 1624, 1697
BsmAI	GTCTC	3 cut(s)	130, 662, 1498
BsmBI	CGTCTC	1 cut(s)	130
BsmFI	GGGAC	3 cut(s)	1086, 1220, 1487
BsmI	GAATGC	3 cut(s)	233, 1002, 1236
BsnI	GGCC	6 cut(s)	335, 666, 861, 1576, 1617, 1717
Bsp119I	TTCGAA	1 cut(s)	826
Bsp1286I	GDGCHC	2 cut(s)	11, 1834
Bsp143I	GATC	5 cut(s)	13, 74, 804, 979, 1043
Bsp1720I	GCTNAGC	1 cut(s)	5
Bsp19I	CCATGG	2 cut(s)	921, 1859
BspACI	CCGC	7 cut(s)	606, 684, 842, 957, 1425, 1565, 1845
BspANI	GGCC	6 cut(s)	335, 666, 861, 1576, 1617, 1717
BspCNI	CTCAG	1 cut(s)	391
BspDI	ATCGAT	1 cut(s)	1046
BspFNI	CGCG	2 cut(s)	801, 1565
BspLI	GGNNCC	3 cut(s)	405, 1168, 1569
BspPI	GGATC	1 cut(s)	1051
BspT104I	TTCGAA	1 cut(s)	826
BspT107I	GGYRCC	1 cut(s)	1567
BspTI	CTTAAG	1 cut(s)	776
BsrBI	CCGCTC	1 cut(s)	1425
BsrDI	GCAATG	1 cut(s)	1872
BsrFI	RCCGGY	1 cut(s)	1753
BsrI	ACTGG	7 cut(s)	667, 685, 867, 1103, 1279, 1790, 1928
BssAI	RCCGGY	1 cut(s)	1753
BssECI	CCNNGG	3 cut(s)	921, 1571, 1859
BssMI	GATC	5 cut(s)	13, 74, 804, 979, 1043
BssNI	GRCGYC	1 cut(s)	1403
BssT1I	CCWWGG	3 cut(s)	921, 1571, 1859
Bst2UI	CCWGG	6 cut(s)	337, 884, 1292, 1578, 1614, 1635
Bst4CI	ACNGT	2 cut(s)	468, 934
Bst6I	CTCTTC	4 cut(s)	479, 484, 1582, 1716
BstACI	GRCGYC	1 cut(s)	1403
BstAFI	CTTAAG	1 cut(s)	776
BstAPI	GCANNNNNTGC	1 cut(s)	191
BstBAI	YACGTR	1 cut(s)	1460
BstBI	TTCGAA	1 cut(s)	826
BstC8I	GCNNGC	1 cut(s)	608
BstDEI	CTNAG	2 cut(s)	5, 399
BstDSI	CCRYGG	2 cut(s)	921, 1859
BstENI	CCTNNNNNAGG	1 cut(s)	1575
BstF5I	GGATG	5 cut(s)	114, 558, 952, 1502, 1892
BstFNI	CGCG	2 cut(s)	801, 1565
BstHHI	GCGC	1 cut(s)	625
BstKTI	GATC	5 cut(s)	16, 77, 807, 982, 1046
BstMAI	GTCTC	3 cut(s)	130, 662, 1498
BstMBI	GATC	5 cut(s)	13, 74, 804, 979, 1043
BstMWI	GCNNNNNNNGC	5 cut(s)	191, 963, 1100, 1225, 1701
BstNI	CCWGG	6 cut(s)	337, 884, 1292, 1578, 1614, 1635
BstSCI	CCNGG	6 cut(s)	335, 882, 1290, 1576, 1612, 1633
BstUI	CGCG	2 cut(s)	801, 1565
BstV1I	GCAGC	5 cut(s)	615, 831, 1615, 1825, 1828
BstV2I	GAAGAC	3 cut(s)	255, 452, 1332
BstXI	CCANNNNNNTGG	1 cut(s)	1274
Bsu15I	ATCGAT	1 cut(s)	1046
BsuRI	GGCC	6 cut(s)	335, 666, 861, 1576, 1617, 1717
BsuTUI	ATCGAT	1 cut(s)	1046
BtgI	CCRYGG	2 cut(s)	921, 1859
BtrI	CACGTC	1 cut(s)	999
BtsCI	GGATG	5 cut(s)	114, 558, 952, 1502, 1892
BtsI	GCAGTG	2 cut(s)	238, 591
BtsIMutI	CAGTG	6 cut(s)	238, 591, 874, 1272, 1783, 1784
Cac8I	GCNNGC	1 cut(s)	608
CaiI	CAGNNNCTG	1 cut(s)	530
CfoI	GCGC	1 cut(s)	625
Cfr10I	RCCGGY	1 cut(s)	1753
Cfr13I	GGNCC	2 cut(s)	1688, 1716
ClaI	ATCGAT	1 cut(s)	1046
Csp6I	GTAC	3 cut(s)	97, 738, 1568
CspAI	ACCGGT	1 cut(s)	1753
CviAII	CATG	7 cut(s)	583, 620, 857, 922, 1241, 1582, 1860
CviQI	GTAC	3 cut(s)	97, 738, 1568
DdeI	CTNAG	2 cut(s)	5, 399
DpnI	GATC	5 cut(s)	15, 76, 806, 981, 1045
DpnII	GATC	5 cut(s)	13, 74, 804, 979, 1043
DraIII	CACNNNGTG	1 cut(s)	125
DrdI	GACNNNNNNGTC	1 cut(s)	1334
DseDI	GACNNNNNNGTC	1 cut(s)	1334
EaeI	YGGCCR	3 cut(s)	664, 859, 1574
Eam1104I	CTCTTC	4 cut(s)	479, 484, 1582, 1716
EarI	CTCTTC	4 cut(s)	479, 484, 1582, 1716
Ecl136II	GAGCTC	1 cut(s)	9
Eco130I	CCWWGG	3 cut(s)	921, 1571, 1859
Eco24I	GRGCYC	1 cut(s)	11
Eco47I	GGWCC	1 cut(s)	1688
Eco53kI	GAGCTC	1 cut(s)	9
Eco57I	CTGAAG	1 cut(s)	1199
Eco72I	CACGTG	1 cut(s)	1460
EcoICRI	GAGCTC	1 cut(s)	9
EcoNI	CCTNNNNNAGG	1 cut(s)	1575
EcoRI	GAATTC	2 cut(s)	424, 828
EcoRII	CCWGG	6 cut(s)	335, 882, 1290, 1576, 1612, 1633
EcoT14I	CCWWGG	3 cut(s)	921, 1571, 1859
EcoT22I	ATGCAT	1 cut(s)	707
EcoT38I	GRGCYC	1 cut(s)	11
ErhI	CCWWGG	3 cut(s)	921, 1571, 1859
Esp3I	CGTCTC	1 cut(s)	130
FaeI	CATG	7 cut(s)	586, 623, 860, 925, 1244, 1585, 1863
FalI	AAGNNNNNCTT	2 cut(s)	1713, 1745
FaqI	GGGAC	3 cut(s)	1086, 1220, 1487
FatI	CATG	7 cut(s)	582, 619, 856, 921, 1240, 1581, 1859
FauI	CCCGC	2 cut(s)	599, 1418
FbaI	TGATCA	1 cut(s)	13
Fnu4HI	GCNGC	5 cut(s)	604, 845, 1629, 1814, 1817
FokI	GGATG	5 cut(s)	121, 565, 959, 1489, 1899
FriOI	GRGCYC	1 cut(s)	11
Fsp4HI	GCNGC	5 cut(s)	604, 845, 1629, 1814, 1817
FspAI	RTGCGCAY	1 cut(s)	624
FspBI	CTAG	1 cut(s)	267
FspI	TGCGCA	1 cut(s)	624
GlaI	GCGC	1 cut(s)	624
GluI	GCNGC	5 cut(s)	604, 845, 1629, 1814, 1817
GsuI	CTGGAG	3 cut(s)	390, 1617, 1643
HaeIII	GGCC	6 cut(s)	335, 666, 861, 1576, 1617, 1717
HapII	CCGG	2 cut(s)	792, 1754
HhaI	GCGC	1 cut(s)	625
Hin1I	GRCGYC	1 cut(s)	1403
Hin1II	CATG	7 cut(s)	586, 623, 860, 925, 1244, 1585, 1863
Hin6I	GCGC	1 cut(s)	623
HinP1I	GCGC	1 cut(s)	623
HincII	GTYRAC	1 cut(s)	1021
HindII	GTYRAC	1 cut(s)	1021
HinfI	GANTC	9 cut(s)	50, 179, 340, 836, 1074, 1328, 1454, 1646, 1850
HpaII	CCGG	2 cut(s)	792, 1754
HphI	GGTGA	8 cut(s)	57, 357, 449, 655, 698, 831, 971, 1793
Hpy166II	GTNNAC	3 cut(s)	591, 1021, 1801
Hpy188III	TCNNGA	9 cut(s)	344, 407, 659, 812, 833, 1112, 1392, 1622, 1903
Hpy8I	GTNNAC	3 cut(s)	591, 1021, 1801
HpyAV	CCTTC	2 cut(s)	378, 1544
HpyCH4III	ACNGT	2 cut(s)	468, 934
HpyCH4IV	ACGT	4 cut(s)	998, 1403, 1459, 1792
HpyF10VI	GCNNNNNNNGC	5 cut(s)	191, 963, 1100, 1225, 1701
HpyF3I	CTNAG	2 cut(s)	5, 399
HpySE526I	ACGT	4 cut(s)	998, 1403, 1459, 1792
Hsp92I	GRCGYC	1 cut(s)	1403
Hsp92II	CATG	7 cut(s)	586, 623, 860, 925, 1244, 1585, 1863
HspAI	GCGC	1 cut(s)	623
KpnI	GGTACC	1 cut(s)	1571
Ksp22I	TGATCA	1 cut(s)	13
Kzo9I	GATC	5 cut(s)	13, 74, 804, 979, 1043
LmnI	GCTCC	4 cut(s)	236, 409, 1599, 1636
Lsp1109I	GCAGC	5 cut(s)	615, 831, 1615, 1825, 1828
LweI	GCATC	4 cut(s)	337, 714, 1208, 1287
MaeI	CTAG	1 cut(s)	267
MaeII	ACGT	4 cut(s)	998, 1403, 1459, 1792
MaeIII	GTNAC	9 cut(s)	63, 308, 715, 837, 959, 994, 1171, 1781, 1889
MalI	GATC	5 cut(s)	15, 76, 806, 981, 1045
MbiI	CCGCTC	1 cut(s)	1425
MboI	GATC	5 cut(s)	13, 74, 804, 979, 1043
MhlI	GDGCHC	2 cut(s)	11, 1834
MlsI	TGGCCA	3 cut(s)	666, 861, 1576
MluCI	AATT	9 cut(s)	102, 328, 424, 615, 828, 901, 1027, 1437, 1669
MluNI	TGGCCA	3 cut(s)	666, 861, 1576
MlyI	GAGTC	6 cut(s)	44, 334, 845, 1068, 1322, 1448
MmeI	TCCRAC	2 cut(s)	401, 1426
Mox20I	TGGCCA	3 cut(s)	666, 861, 1576
Mph1103I	ATGCAT	1 cut(s)	707
MscI	TGGCCA	3 cut(s)	666, 861, 1576
MseI	TTAA	5 cut(s)	129, 722, 746, 777, 1682
MslI	CAYNNNNRTG	3 cut(s)	641, 867, 1191
Msp20I	TGGCCA	3 cut(s)	666, 861, 1576
MspA1I	CMGCKG	5 cut(s)	606, 844, 1219, 1847, 1883
MspCI	CTTAAG	1 cut(s)	776
MspI	CCGG	2 cut(s)	792, 1754
MspR9I	CCNGG	6 cut(s)	337, 884, 1292, 1578, 1614, 1635
Mva1269I	GAATGC	3 cut(s)	233, 1002, 1236
MvaI	CCWGG	6 cut(s)	337, 884, 1292, 1578, 1614, 1635
MvnI	CGCG	2 cut(s)	801, 1565
MwoI	GCNNNNNNNGC	5 cut(s)	191, 963, 1100, 1225, 1701
NcoI	CCATGG	2 cut(s)	921, 1859
NdeII	GATC	5 cut(s)	13, 74, 804, 979, 1043
NlaIII	CATG	7 cut(s)	586, 623, 860, 925, 1244, 1585, 1863
NlaIV	GGNNCC	3 cut(s)	405, 1168, 1569
NmuCI	GTSAC	6 cut(s)	63, 837, 959, 994, 1781, 1889
NsbI	TGCGCA	1 cut(s)	624
NsiI	ATGCAT	1 cut(s)	707
NspV	TTCGAA	1 cut(s)	826
PctI	GAATGC	3 cut(s)	233, 1002, 1236
PfeI	GAWTC	3 cut(s)	179, 1646, 1850
PfoI	TCCNGGA	1 cut(s)	1633
PinAI	ACCGGT	1 cut(s)	1753
PkrI	GCNGC	5 cut(s)	605, 846, 1630, 1815, 1818
PleI	GAGTC	6 cut(s)	44, 334, 844, 1068, 1322, 1448
PmaCI	CACGTG	1 cut(s)	1460
PmlI	CACGTG	1 cut(s)	1460
PpsI	GAGTC	6 cut(s)	44, 334, 844, 1068, 1322, 1448
Ppu21I	YACGTR	1 cut(s)	1460
PsiI	TTATAA	2 cut(s)	473, 1121
Psp124BI	GAGCTC	1 cut(s)	11
Psp6I	CCWGG	6 cut(s)	335, 882, 1290, 1576, 1612, 1633
PspCI	CACGTG	1 cut(s)	1460
PspGI	CCWGG	6 cut(s)	335, 882, 1290, 1576, 1612, 1633
PspN4I	GGNNCC	3 cut(s)	405, 1168, 1569
PspPI	GGNCC	2 cut(s)	1688, 1716
PstNI	CAGNNNCTG	1 cut(s)	530
PvuII	CAGCTG	2 cut(s)	1219, 1883
RsaI	GTAC	3 cut(s)	98, 739, 1569
RsaNI	GTAC	3 cut(s)	97, 738, 1568
RseI	CAYNNNNRTG	3 cut(s)	641, 867, 1191
SacI	GAGCTC	1 cut(s)	11
SaqAI	TTAA	5 cut(s)	129, 722, 746, 777, 1682
SatI	GCNGC	5 cut(s)	604, 845, 1629, 1814, 1817
Sau3AI	GATC	5 cut(s)	13, 74, 804, 979, 1043
Sau96I	GGNCC	2 cut(s)	1688, 1716
SchI	GAGTC	6 cut(s)	44, 334, 845, 1068, 1322, 1448
ScrFI	CCNGG	6 cut(s)	337, 884, 1292, 1578, 1614, 1635
SduI	GDGCHC	2 cut(s)	11, 1834
SfaNI	GCATC	4 cut(s)	337, 714, 1208, 1287
SfuI	TTCGAA	1 cut(s)	826
SinI	GGWCC	1 cut(s)	1688
SmiMI	CAYNNNNRTG	3 cut(s)	641, 867, 1191
SmlI	CTYRAG	2 cut(s)	753, 776
SmoI	CTYRAG	2 cut(s)	753, 776
Sse9I	AATT	9 cut(s)	102, 328, 424, 615, 828, 901, 1027, 1437, 1669
SsiI	CCGC	7 cut(s)	606, 684, 842, 957, 1425, 1565, 1845
SspMI	CTAG	1 cut(s)	267
SstI	GAGCTC	1 cut(s)	11
StyD4I	CCNGG	6 cut(s)	335, 882, 1290, 1576, 1612, 1633
StyI	CCWWGG	3 cut(s)	921, 1571, 1859
TaaI	ACNGT	2 cut(s)	468, 934
TaiI	ACGT	4 cut(s)	1001, 1406, 1462, 1795
TasI	AATT	9 cut(s)	102, 328, 424, 615, 828, 901, 1027, 1437, 1669
TatI	WGTACW	1 cut(s)	96
TfiI	GAWTC	3 cut(s)	179, 1646, 1850
Tru1I	TTAA	5 cut(s)	129, 722, 746, 777, 1682
Tru9I	TTAA	5 cut(s)	129, 722, 746, 777, 1682
TscAI	CASTG	6 cut(s)	238, 598, 874, 1279, 1784, 1790
TseFI	GTSAC	6 cut(s)	63, 837, 959, 994, 1781, 1889
TseI	GCWGC	5 cut(s)	603, 844, 1628, 1813, 1816
Tsp45I	GTSAC	6 cut(s)	63, 837, 959, 994, 1781, 1889
TspGWI	ACGGA	6 cut(s)	1159, 1328, 1365, 1680, 1908, 1935
TspRI	CASTG	6 cut(s)	238, 598, 874, 1279, 1784, 1790
Vha464I	CTTAAG	1 cut(s)	776
VpaK11BI	GGWCC	1 cut(s)	1688
XagI	CCTNNNNNAGG	1 cut(s)	1575
XapI	RAATTY	5 cut(s)	102, 328, 424, 615, 828
XspI	CTAG	1 cut(s)	267
ZraI	GACGTC	1 cut(s)	1404
Zsp2I	ATGCAT	1 cut(s)	707

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.