Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0005400

Physical Location & Seq

Genomic Coordinates Reverse (-)

18065829 .. 18071634

Gene Structure 5806 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g26240.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2556 bp

ATGGCGCTGCCGCACTCTCCTCTCTTGGAGCCTCATTCCCTCTCACCTCTCTCCTCCAAGTTCCGCCACGTCATCCCCCTCAACAACTGCCTCCGTACTTCCTTCTTCGCCGGGAAAGCCTTCCGCCTGCCGGAAACCTCACGGATTTCGAGCCGGAGACGCCGGCGAGCGCAGGCGGCGGCTTCTCTGGGAGGTCTTTTCGGCGGGATTTTCAAAGGGACTGACACTGGCGAGTCCACAAGGCAGCAGTACGCTCAGACTGTGGCTCTCATCAATGGCTTGGAATCTCAGATTTCCAAGCTTTCCGATTCCGAGCTCAGGGAGAAGACGCTTCAGTTTCAGCAGCGCGCTAAGCAGGGCGAGTCCTTGGACTCTCTTTTACCCGAAGCATTTGCCGTCATAAGAGAGGCTTCGAGGAGGGTTTTAGGCCTTCGTCCTTTTGATGTACAACTCATAGGTGGCATGGTTCTTCACAAGGGGGAAATAGCTGAGATGAGGACCGGAGAAGGAAAGACCCTTGTTGCTATCTTACCCGCTTATTTGAATGCCTTGACTGGAAAGGGAGTTCATGTTGTCACTGTCAATGATTATCTAGCCCGACGTGATTGTGAGTGGGTTGGTCAAGTTCCTCGTTTCCTTGGATTGAAGAGTGTTGAGGAGCTTGTCTTGAGGAATTTCAATTACTGTGTCATTGATGAGGTTGATTCCATCCTTATTGATGAAGCAAGAACACCCCTCATTATATCAGGACCTGCAGAAAAACCCAGCGATAGGTACTATAAAGCTGCAAAGATGGCTTCAGTGTTTGAGCGAGATATACATTATACTGTAGATGAAAAGCAGAAAACAGTTCTGCTTTCAGAACAAGGATATGAAGATGCTGAAGAGATTCTAGGTGTCAAAGATTTATATGATCCACGTGAACAGTGGGCATCTTATGTTCTGAATGCAGTGAAAGCAAAAGAACTGTTTCTCAGAGATGTAAATTATATTATTCGTGGCAAAGAGGTTCTCATTGTTGATGAATTTACTGGACGAGTTATGCAGGGGAGGCGGTGGAGTGATGGCCTTCACCAAGCAGTTGAAGCAAAAGAGGGTCTTCCAATTCAAAATGAAACTGTAACTCTGGCATCTATTAGCTACCAAAACTTCTTTCTCCAGTTTCCAAAACTTTGTGGAATGACTGGGACTGCAGCAACAGAAAGCACAGAATTTGAAAGCATATACAAGCTTAAAGTTACAATTGTCCCCACAAACAAGCCTATGATAAGAAAGGATGAGTCTGATGTAGTTTTCAGGGCAACTACAGGAAAATGGCGGGCAGTTGTAGTGGAGATATCTAGAATGCACAAAACAGGTCGCCCTGTACTTGTTGGAACAACTAGTGTTGAGCAGAGTGACTCATTGTCTGAGCAGTTGCAAGAAGTTGGGATTCCCCATGAGGTTCTCAACGCAAAACCAGAGAATGTGGAGAGGGAAGCAGAAATTGTGGCACAAAGCGGTCGTCTAGGAGCAGTTACCATTGCAACCAATATGGCAGGTCGAGGAACAGACATAATTCTTGGTGGGAATGCAGAATTTATGGCAAGGTTGAAGCTACGTGAGATGCTAATGCCTAGAGTTGTAAAACTAACTGAAGGAGGTTATGTTTCAGTGAAAAAACTTCCACCGAAAAAGTCTTGGAAGGTTAATGAAAAGCTATTTCCATGCAAATTGTCCAGTGAAAAGACCAAGTTAGCTGAGGAAGCTGTCAATTTAGCTGTTGAAACTTGGGGTCAGAGATCATTGACAGAGCTTGAAGCAGAGGAGCGTCTTTCTTATTCTTGCGAAAAGGGACCTGCTTTGGATGATGTGATAGCTAAACTGCGGAGTGCTTTTCTAGAAATCATGAAAGAATATAAGGGCTACACAGAGGAAGAAAGGAAGAAGGTTGTGTCAGCTGGTGGGCTTCATGTTGTTGGGACAGAACGCCATGAATCTCGCCGAGTTGATAATCAGTTGCGTGGTCGAACTGGACGGCAGGGGGATCCTGGCAGTTCACGCTTCTTCTTAAGTCTTGAAGATAACATCTTTCGTATATTTGGTGGAGATCGCATTCAGGGTTTAATGAGAGCTTTTAGAGTTGAGGACTTGCCAATCGAGTCCAAGATGCTGACCAAAGCACTAGATGAAGCTCAGCGGAAAGTGGAAAATTATTTTTTCGATATTCGAAAGCAACTATTTGAGTTTGATGAAGTCCTAAACAGCCAAAGAGACCGTGTGTATACGGAAAGAAGGAGAGCCCTTGAATCTGACAATCTGCAATCACTTATAATTGAATATGCGGAACTAACTATGGATGATATCTTAGAGGCAAATATTGGTTCAGATGCTCCGAAAGAAAGCTGGGATCTTGACAAGCTCATCAAGAAACTTCAACAATATTGCTATCTATTGAATGATCTGACGCCGGATGTGCTGAGTAGTGAATGTTCAAGTTACGAAGATTTGCAGGACTACCTCCGTCTTCGAGGTCGTGAAGCATATTTGCAGAAAAGGTCTTCAAAGTACGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071806 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW

Protein Analysis

852

Amino Acids

96.17

Weight (kDa)

6.89

Isoelectric Point (pI)

48.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SecA_DEAD	PF07517	78 - 216	9.4e-58	SecA DEAD-like domain
SecA_DEAD	PF07517	217 - 406	1.7e-36	SecA DEAD-like domain
SecA_PP_bind	PF01043	256 - 362	8.1e-38	SecA preprotein cross-linking domain
P-loop_SecA	PF21090	421 - 710	1.1e-82	SecA P-loop domain
SecA_SW	PF07516	712 - 845	4.8e-28	SecA Wing and Scaffold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01800 AT4G01800 AT4G01800
fragaria_vesca	FvH4_1g26240
malus_domestica	MD17G1285000.v1.1
prunus_persica	Prupe.3G002700_v2.0.a1
pyrus_communis	pycom09g19850 pycom17g28080 pycom17g28090
rosa_chinensis	RchiOBHm_Chr3g0485781
rosa_laevigata	RLG00000023125
rosa_multiflora	Rmu_co8471925.1_g000001 Rmu_sc0012870.1_g000002
rosa_roxburghii	Rroxscaffold_2G00123840
rosa_rugosa	Rorug03G0221400
rosa_samantha	Rh1AG180100 Rh1CG167100 Rh3AG270700 Rh3BG306200 Rh3BG306300 Rh3CG304600 Rh3DG300700 Rh4AG241300 Rh4AG241400 Rh4BG244600 Rh4BG244700 Rh4CG255400 Rh7CG119700
rosa_wichuraiana	Rw3G024110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2312
Acc36I	ACCTGC	3 cut(s)	760, 1529, 1846
AccI	GTMKAC	1 cut(s)	2264
AccII	CGCG	1 cut(s)	348
AclWI	GGATC	4 cut(s)	908, 2021, 2034, 2397
AcsI	RAATTY	4 cut(s)	673, 1025, 1211, 1577
AcuI	CTGAAG	4 cut(s)	317, 783, 903, 1656
AcvI	CACGTG	1 cut(s)	920
AcyI	GRCGYC	2 cut(s)	160, 2447
AfaI	GTAC	6 cut(s)	97, 251, 447, 776, 1368, 2549
AfiI	CCNNNNNNNGG	3 cut(s)	130, 318, 771
AflII	CTTAAG	1 cut(s)	2050
AhdI	GACNNNNNGTC	1 cut(s)	1405
AhlI	ACTAGT	1 cut(s)	1382
AjiI	CACGTC	2 cut(s)	70, 602
AjnI	CCWGG	1 cut(s)	2029
AjuI	GAANNNNNNNTTGG	2 cut(s)	1137, 1169
AloI	GAACNNNNNNTCC	2 cut(s)	549, 581
Alw21I	GWGCWC	1 cut(s)	318
Alw26I	GTCTC	2 cut(s)	151, 2247
AlwI	GGATC	4 cut(s)	908, 2021, 2034, 2397
AlwNI	CAGNNNCTG	1 cut(s)	752
AoxI	GGCC	2 cut(s)	427, 1066
ApeKI	GCWGC	5 cut(s)	7, 244, 343, 785, 1193
ApoI	RAATTY	4 cut(s)	673, 1025, 1211, 1577
ArsI	GACNNNNNNTTYG	2 cut(s)	1489, 1521
Asp700I	GAANNNNTTC	3 cut(s)	119, 888, 969
AspLEI	GCGC	4 cut(s)	7, 172, 348, 350
AspS9I	GGNCC	3 cut(s)	498, 749, 1835
AsuC2I	CCSGG	1 cut(s)	112
AsuHPI	GGTGA	2 cut(s)	36, 1064
AsuII	TTCGAA	1 cut(s)	2209
AvaII	GGWCC	3 cut(s)	498, 749, 1835
BamHI	GGATCC	1 cut(s)	2026
BanII	GRGCYC	2 cut(s)	318, 2284
BbrPI	CACGTG	1 cut(s)	920
BbsI	GAAGAC	4 cut(s)	332, 1091, 2498, 2532
Bbv12I	GWGCWC	1 cut(s)	318
BbvCI	CCTCAGC	1 cut(s)	1740
BbvI	GCAGC	4 cut(s)	256, 355, 772, 1205
BccI	CCATC	3 cut(s)	716, 787, 1058
BceAI	ACGGC	2 cut(s)	380, 2033
BcgI	CGANNNNNNTGC	2 cut(s)	374, 408
BciT130I	CCWGG	1 cut(s)	2031
BcnI	CCSGG	1 cut(s)	112
BcoDI	GTCTC	2 cut(s)	151, 2247
BcuI	ACTAGT	1 cut(s)	1382
BfaI	CTAG	8 cut(s)	593, 893, 1341, 1383, 1508, 1617, 1880, 2165
BfmI	CTRYAG	4 cut(s)	753, 828, 1191, 1305
BfoI	RGCGCY	1 cut(s)	8
BfrI	CTTAAG	1 cut(s)	2050
BfuAI	ACCTGC	3 cut(s)	760, 1529, 1846
BisI	GCNGC	8 cut(s)	8, 11, 177, 180, 245, 344, 786, 1194
BlpI	GCTNAGC	2 cut(s)	351, 2175
BlsI	GCNGC	8 cut(s)	9, 12, 178, 181, 246, 345, 787, 1195
Bme1390I	CCNGG	2 cut(s)	112, 2031
Bme18I	GGWCC	3 cut(s)	498, 749, 1835
BmeRI	GACNNNNNGTC	1 cut(s)	1405
BmgBI	CACGTC	2 cut(s)	70, 602
BmgT120I	GGNCC	3 cut(s)	498, 749, 1835
BmiI	GGNNCC	3 cut(s)	30, 1836, 2028
BmrFI	CCNGG	2 cut(s)	112, 2031
BmrI	ACTGGG	1 cut(s)	1194
BmsI	GCATC	6 cut(s)	868, 941, 1139, 1596, 2139, 2359
BmuI	ACTGGG	1 cut(s)	1194
BpiI	GAAGAC	4 cut(s)	332, 1091, 2498, 2532
BpmI	CTGGAG	1 cut(s)	1142
Bpu10I	CCTNAGC	2 cut(s)	317, 1740
Bpu1102I	GCTNAGC	2 cut(s)	351, 2175
Bpu14I	TTCGAA	1 cut(s)	2209
BpuEI	CTTGAG	1 cut(s)	688
BpuMI	CCSGG	1 cut(s)	112
BsaAI	YACGTR	2 cut(s)	920, 1601
BsaHI	GRCGYC	2 cut(s)	160, 2447
BsaI	GGTCTC	1 cut(s)	2247
BsaJI	CCNNGG	2 cut(s)	366, 637
BsaWI	WCCGGW	1 cut(s)	500
BsaXI	ACNNNNNCTCC	4 cut(s)	555, 585, 1632, 1662
Bsc4I	CCNNNNNNNGG	3 cut(s)	130, 318, 771
Bse118I	RCCGGY	1 cut(s)	162
Bse1I	ACTGG	7 cut(s)	232, 559, 1036, 1159, 1189, 1719, 2017
Bse3DI	GCAATG	1 cut(s)	1521
BseBI	CCWGG	1 cut(s)	2031
BseDI	CCNNGG	2 cut(s)	366, 637
BseGI	GGATG	6 cut(s)	72, 708, 1282, 1852, 2344, 2458
BseLI	CCNNNNNNNGG	3 cut(s)	130, 318, 771
BseMI	GCAATG	1 cut(s)	1521
BseMII	CTCAG	9 cut(s)	269, 302, 331, 480, 988, 1401, 1731, 2189, 2450
BseNI	ACTGG	7 cut(s)	232, 559, 1036, 1159, 1189, 1719, 2017
BsePI	GCGCGC	1 cut(s)	346
BseRI	GAGGAG	5 cut(s)	9, 43, 430, 671, 1820
BseXI	GCAGC	4 cut(s)	256, 355, 772, 1205
BseYI	CCCAGC	2 cut(s)	764, 2385
Bsh1236I	CGCG	1 cut(s)	348
Bsh1285I	CGRYCG	1 cut(s)	1504
BshFI	GGCC	2 cut(s)	429, 1068
BsiEI	CGRYCG	1 cut(s)	1504
BsiHKAI	GWGCWC	1 cut(s)	318
BsiSI	CCGG	6 cut(s)	111, 131, 154, 163, 501, 2450
BslFI	GGGAC	5 cut(s)	232, 1201, 1232, 1848, 1975
BslI	CCNNNNNNNGG	3 cut(s)	130, 318, 771
BsmAI	GTCTC	2 cut(s)	151, 2247
BsmBI	CGTCTC	1 cut(s)	151
BsmFI	GGGAC	5 cut(s)	232, 1201, 1232, 1848, 1975
BsmI	GAATGC	5 cut(s)	550, 952, 1350, 1576, 2094
BsnI	GGCC	2 cut(s)	429, 1068
Bso31I	GGTCTC	1 cut(s)	2247
Bsp119I	TTCGAA	1 cut(s)	2209
Bsp1286I	GDGCHC	2 cut(s)	318, 2284
Bsp1407I	TGTACA	1 cut(s)	445
Bsp143I	GATC	6 cut(s)	913, 1781, 2026, 2089, 2389, 2440
Bsp1720I	GCTNAGC	2 cut(s)	351, 2175
BspANI	GGCC	2 cut(s)	429, 1068
BspCNI	CTCAG	9 cut(s)	268, 301, 330, 481, 987, 1402, 1732, 2188, 2451
BspFNI	CGCG	1 cut(s)	348
BspHI	TCATGA	1 cut(s)	1887
BspLI	GGNNCC	3 cut(s)	30, 1836, 2028
BspMAI	CTGCAG	2 cut(s)	757, 1195
BspMI	ACCTGC	3 cut(s)	760, 1529, 1846
BspPI	GGATC	4 cut(s)	908, 2021, 2034, 2397
BspT104I	TTCGAA	1 cut(s)	2209
BspTI	CTTAAG	1 cut(s)	2050
BspTNI	GGTCTC	1 cut(s)	2247
BsrDI	GCAATG	1 cut(s)	1521
BsrFI	RCCGGY	1 cut(s)	162
BsrGI	TGTACA	1 cut(s)	445
BsrI	ACTGG	7 cut(s)	232, 559, 1036, 1159, 1189, 1719, 2017
BssAI	RCCGGY	1 cut(s)	162
BssECI	CCNNGG	2 cut(s)	366, 637
BssHII	GCGCGC	1 cut(s)	346
BssMI	GATC	6 cut(s)	913, 1781, 2026, 2089, 2389, 2440
BssNAI	GTATAC	1 cut(s)	2265
BssNI	GRCGYC	2 cut(s)	160, 2447
BssT1I	CCWWGG	2 cut(s)	366, 637
Bst1107I	GTATAC	1 cut(s)	2265
Bst2UI	CCWGG	1 cut(s)	2031
Bst4CI	ACNGT	9 cut(s)	262, 580, 686, 829, 850, 927, 969, 1120, 2258
Bst6I	CTCTTC	2 cut(s)	641, 879
BstACI	GRCGYC	2 cut(s)	160, 2447
BstAFI	CTTAAG	1 cut(s)	2050
BstAUI	TGTACA	1 cut(s)	445
BstBAI	YACGTR	2 cut(s)	920, 1601
BstBI	TTCGAA	1 cut(s)	2209
BstC8I	GCNNGC	6 cut(s)	128, 164, 168, 174, 348, 1320
BstF5I	GGATG	6 cut(s)	72, 708, 1282, 1852, 2344, 2458
BstFNI	CGCG	1 cut(s)	348
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	4 cut(s)	7, 172, 348, 350
BstKTI	GATC	6 cut(s)	916, 1784, 2029, 2092, 2392, 2443
BstMAI	GTCTC	2 cut(s)	151, 2247
BstMBI	GATC	6 cut(s)	913, 1781, 2026, 2089, 2389, 2440
BstMCI	CGRYCG	1 cut(s)	1504
BstNI	CCWGG	1 cut(s)	2031
BstSCI	CCNGG	2 cut(s)	110, 2029
BstSFI	CTRYAG	4 cut(s)	753, 828, 1191, 1305
BstUI	CGCG	1 cut(s)	348
BstV1I	GCAGC	4 cut(s)	256, 355, 772, 1205
BstV2I	GAAGAC	4 cut(s)	332, 1091, 2498, 2532
BstX2I	RGATCY	2 cut(s)	2026, 2389
BstYI	RGATCY	2 cut(s)	2026, 2389
BstZ17I	GTATAC	1 cut(s)	2265
BsuRI	GGCC	2 cut(s)	429, 1068
BtrI	CACGTC	2 cut(s)	70, 602
BtsCI	GGATG	6 cut(s)	72, 708, 1282, 1852, 2344, 2458
BtsI	GCAGTG	1 cut(s)	957
BtsIMutI	CAGTG	7 cut(s)	225, 576, 807, 932, 957, 1659, 1726
BveI	ACCTGC	3 cut(s)	760, 1529, 1846
Cac8I	GCNNGC	6 cut(s)	128, 164, 168, 174, 348, 1320
CaiI	CAGNNNCTG	1 cut(s)	752
CciI	TCATGA	1 cut(s)	1887
CfoI	GCGC	4 cut(s)	7, 172, 348, 350
Cfr10I	RCCGGY	1 cut(s)	162
Cfr13I	GGNCC	3 cut(s)	498, 749, 1835
CseI	GACGC	4 cut(s)	168, 337, 1799, 2455
Csp6I	GTAC	6 cut(s)	96, 250, 446, 775, 1367, 2548
CviAII	CATG	7 cut(s)	463, 569, 1439, 1707, 1888, 1952, 1973
CviQI	GTAC	6 cut(s)	96, 250, 446, 775, 1367, 2548
DpnI	GATC	6 cut(s)	915, 1783, 2028, 2091, 2391, 2442
DpnII	GATC	6 cut(s)	913, 1781, 2026, 2089, 2389, 2440
DriI	GACNNNNNGTC	1 cut(s)	1405
Eam1104I	CTCTTC	2 cut(s)	641, 879
Eam1105I	GACNNNNNGTC	1 cut(s)	1405
EarI	CTCTTC	2 cut(s)	641, 879
EciI	GGCGGA	2 cut(s)	53, 113
Ecl136II	GAGCTC	1 cut(s)	316
Eco130I	CCWWGG	2 cut(s)	366, 637
Eco147I	AGGCCT	1 cut(s)	429
Eco24I	GRGCYC	2 cut(s)	318, 2284
Eco31I	GGTCTC	1 cut(s)	2247
Eco32I	GATATC	2 cut(s)	1338, 2344
Eco47I	GGWCC	3 cut(s)	498, 749, 1835
Eco53kI	GAGCTC	1 cut(s)	316
Eco57I	CTGAAG	4 cut(s)	317, 783, 903, 1656
Eco72I	CACGTG	1 cut(s)	920
EcoICRI	GAGCTC	1 cut(s)	316
EcoO109I	RGGNCCY	2 cut(s)	749, 1835
EcoRII	CCWGG	1 cut(s)	2029
EcoRV	GATATC	2 cut(s)	1338, 2344
EcoT14I	CCWWGG	2 cut(s)	366, 637
EcoT38I	GRGCYC	2 cut(s)	318, 2284
ErhI	CCWWGG	2 cut(s)	366, 637
Esp3I	CGTCTC	1 cut(s)	151
FaeI	CATG	7 cut(s)	466, 572, 1442, 1710, 1891, 1955, 1976
FalI	AAGNNNNNCTT	4 cut(s)	394, 426, 1083, 1115
FaqI	GGGAC	5 cut(s)	232, 1201, 1232, 1848, 1975
FatI	CATG	7 cut(s)	462, 568, 1438, 1706, 1887, 1951, 1972
FauI	CCCGC	3 cut(s)	197, 541, 1311
FblI	GTMKAC	1 cut(s)	2264
Fnu4HI	GCNGC	8 cut(s)	8, 11, 177, 180, 245, 344, 786, 1194
FokI	GGATG	6 cut(s)	59, 695, 1289, 1859, 2351, 2465
FriOI	GRGCYC	2 cut(s)	318, 2284
Fsp4HI	GCNGC	8 cut(s)	8, 11, 177, 180, 245, 344, 786, 1194
FspBI	CTAG	8 cut(s)	593, 893, 1341, 1383, 1508, 1617, 1880, 2165
GlaI	GCGC	4 cut(s)	6, 171, 347, 349
GluI	GCNGC	8 cut(s)	8, 11, 177, 180, 245, 344, 786, 1194
GsaI	CCCAGC	2 cut(s)	768, 2389
GsuI	CTGGAG	1 cut(s)	1142
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	2 cut(s)	429, 1068
HapII	CCGG	6 cut(s)	111, 131, 154, 163, 501, 2450
HgaI	GACGC	4 cut(s)	168, 337, 1799, 2455
HhaI	GCGC	4 cut(s)	7, 172, 348, 350
Hin1I	GRCGYC	2 cut(s)	160, 2447
Hin1II	CATG	7 cut(s)	466, 572, 1442, 1710, 1891, 1955, 1976
Hin6I	GCGC	4 cut(s)	5, 170, 346, 348
HinP1I	GCGC	4 cut(s)	5, 170, 346, 348
HindIII	AAGCTT	2 cut(s)	299, 1229
HpaII	CCGG	6 cut(s)	111, 131, 154, 163, 501, 2450
HphI	GGTGA	2 cut(s)	36, 1064
Hpy166II	GTNNAC	4 cut(s)	237, 923, 2039, 2265
Hpy188III	TCNNGA	9 cut(s)	667, 747, 1341, 1880, 1888, 2057, 2393, 2407, 2516
Hpy8I	GTNNAC	4 cut(s)	237, 923, 2039, 2265
Hpy99I	CGWCG	1 cut(s)	603
HpyAV	CCTTC	9 cut(s)	112, 130, 440, 500, 1079, 1631, 1678, 1921, 2268
HpyCH4III	ACNGT	9 cut(s)	262, 580, 686, 829, 850, 927, 969, 1120, 2258
HpyCH4IV	ACGT	4 cut(s)	69, 601, 919, 1600
HpySE526I	ACGT	4 cut(s)	69, 601, 919, 1600
Hsp92I	GRCGYC	2 cut(s)	160, 2447
Hsp92II	CATG	7 cut(s)	466, 572, 1442, 1710, 1891, 1955, 1976
HspAI	GCGC	4 cut(s)	5, 170, 346, 348
KroI	GCCGGC	1 cut(s)	162
KroNI	GCCGGC	1 cut(s)	164
Kzo9I	GATC	6 cut(s)	913, 1781, 2026, 2089, 2389, 2440
LmnI	GCTCC	5 cut(s)	28, 658, 1511, 1807, 2377
Lsp1109I	GCAGC	4 cut(s)	256, 355, 772, 1205
LweI	GCATC	6 cut(s)	868, 941, 1139, 1596, 2139, 2359
MaeI	CTAG	8 cut(s)	593, 893, 1341, 1383, 1508, 1617, 1880, 2165
MaeII	ACGT	4 cut(s)	69, 601, 919, 1600
MaeIII	GTNAC	6 cut(s)	574, 1120, 1237, 1397, 1516, 2477
MalI	GATC	6 cut(s)	915, 1783, 2028, 2091, 2391, 2442
MboI	GATC	6 cut(s)	913, 1781, 2026, 2089, 2389, 2440
MfeI	CAATTG	1 cut(s)	1242
MflI	RGATCY	2 cut(s)	2026, 2389
MhlI	GDGCHC	2 cut(s)	318, 2284
MlyI	GAGTC	6 cut(s)	242, 365, 371, 1289, 1394, 2150
MmeI	TCCRAC	1 cut(s)	1354
MreI	CGCCGGCG	1 cut(s)	162
MroNI	GCCGGC	1 cut(s)	162
MroXI	GAANNNNTTC	3 cut(s)	119, 888, 969
MseI	TTAA	4 cut(s)	1233, 1689, 2051, 2105
MspA1I	CMGCKG	2 cut(s)	1940, 2179
MspCI	CTTAAG	1 cut(s)	2050
MspI	CCGG	6 cut(s)	111, 131, 154, 163, 501, 2450
MspR9I	CCNGG	2 cut(s)	112, 2031
MunI	CAATTG	1 cut(s)	1242
Mva1269I	GAATGC	5 cut(s)	550, 952, 1350, 1576, 2094
MvaI	CCWGG	1 cut(s)	2031
MvnI	CGCG	1 cut(s)	348
NaeI	GCCGGC	1 cut(s)	164
NciI	CCSGG	1 cut(s)	112
NdeII	GATC	6 cut(s)	913, 1781, 2026, 2089, 2389, 2440
NgoMIV	GCCGGC	1 cut(s)	162
NlaIII	CATG	7 cut(s)	466, 572, 1442, 1710, 1891, 1955, 1976
NlaIV	GGNNCC	3 cut(s)	30, 1836, 2028
NmeAIII	GCCGAG	1 cut(s)	2009
NmuCI	GTSAC	2 cut(s)	574, 1397
NspV	TTCGAA	1 cut(s)	2209
PagI	TCATGA	1 cut(s)	1887
PauI	GCGCGC	1 cut(s)	346
PceI	AGGCCT	1 cut(s)	429
PctI	GAATGC	5 cut(s)	550, 952, 1350, 1576, 2094
PdiI	GCCGGC	1 cut(s)	164
PdmI	GAANNNNTTC	3 cut(s)	119, 888, 969
PfeI	GAWTC	7 cut(s)	284, 308, 704, 889, 1432, 1976, 2288
PkrI	GCNGC	8 cut(s)	9, 12, 178, 181, 246, 345, 787, 1195
PleI	GAGTC	6 cut(s)	241, 365, 370, 1288, 1394, 2149
PmaCI	CACGTG	1 cut(s)	920
PmlI	CACGTG	1 cut(s)	920
PpsI	GAGTC	6 cut(s)	241, 365, 370, 1288, 1394, 2149
Ppu21I	YACGTR	2 cut(s)	920, 1601
PpuMI	RGGWCCY	2 cut(s)	749, 1835
PsiI	TTATAA	1 cut(s)	2312
Psp124BI	GAGCTC	1 cut(s)	318
Psp5II	RGGWCCY	2 cut(s)	749, 1835
Psp6I	CCWGG	1 cut(s)	2029
PspCI	CACGTG	1 cut(s)	920
PspFI	CCCAGC	2 cut(s)	764, 2385
PspGI	CCWGG	1 cut(s)	2029
PspN4I	GGNNCC	3 cut(s)	30, 1836, 2028
PspPI	GGNCC	3 cut(s)	498, 749, 1835
PspPPI	RGGWCCY	2 cut(s)	749, 1835
PsrI	GAACNNNNNNTAC	2 cut(s)	2455, 2487
PstI	CTGCAG	2 cut(s)	757, 1195
PstNI	CAGNNNCTG	1 cut(s)	752
PsuI	RGATCY	2 cut(s)	2026, 2389
PteI	GCGCGC	1 cut(s)	346
PvuII	CAGCTG	1 cut(s)	1940
RsaI	GTAC	6 cut(s)	97, 251, 447, 776, 1368, 2549
RsaNI	GTAC	6 cut(s)	96, 250, 446, 775, 1367, 2548
SacI	GAGCTC	1 cut(s)	318
SaqAI	TTAA	4 cut(s)	1233, 1689, 2051, 2105
SatI	GCNGC	8 cut(s)	8, 11, 177, 180, 245, 344, 786, 1194
Sau3AI	GATC	6 cut(s)	913, 1781, 2026, 2089, 2389, 2440
Sau96I	GGNCC	3 cut(s)	498, 749, 1835
SchI	GAGTC	6 cut(s)	242, 365, 371, 1289, 1394, 2150
ScrFI	CCNGG	2 cut(s)	112, 2031
SduI	GDGCHC	2 cut(s)	318, 2284
SfaNI	GCATC	6 cut(s)	868, 941, 1139, 1596, 2139, 2359
SfcI	CTRYAG	4 cut(s)	753, 828, 1191, 1305
SfuI	TTCGAA	1 cut(s)	2209
SgrAI	CRCCGGYG	1 cut(s)	162
SinI	GGWCC	3 cut(s)	498, 749, 1835
SmlI	CTYRAG	2 cut(s)	667, 2050
SmoI	CTYRAG	2 cut(s)	667, 2050
SpeI	ACTAGT	1 cut(s)	1382
SseBI	AGGCCT	1 cut(s)	429
SspI	AATATT	2 cut(s)	2359, 2423
SspMI	CTAG	8 cut(s)	593, 893, 1341, 1383, 1508, 1617, 1880, 2165
SstI	GAGCTC	1 cut(s)	318
StuI	AGGCCT	1 cut(s)	429
StyD4I	CCNGG	2 cut(s)	110, 2029
StyI	CCWWGG	2 cut(s)	366, 637
TaaI	ACNGT	9 cut(s)	262, 580, 686, 829, 850, 927, 969, 1120, 2258
TaiI	ACGT	4 cut(s)	72, 604, 922, 1603
TaqI	TCGA	8 cut(s)	149, 413, 1543, 2008, 2139, 2202, 2209, 2509
TatI	WGTACW	2 cut(s)	445, 1366
TauI	GCSGC	3 cut(s)	13, 179, 182
TfiI	GAWTC	7 cut(s)	284, 308, 704, 889, 1432, 1976, 2288
Tru1I	TTAA	4 cut(s)	1233, 1689, 2051, 2105
Tru9I	TTAA	4 cut(s)	1233, 1689, 2051, 2105
TscAI	CASTG	7 cut(s)	232, 583, 807, 932, 957, 1659, 1726
TseFI	GTSAC	2 cut(s)	574, 1397
TseI	GCWGC	5 cut(s)	7, 244, 343, 785, 1193
Tsp45I	GTSAC	2 cut(s)	574, 1397
TspGWI	ACGGA	4 cut(s)	83, 157, 2282, 2492
TspRI	CASTG	7 cut(s)	232, 583, 807, 932, 957, 1659, 1726
Vha464I	CTTAAG	1 cut(s)	2050
VpaK11BI	GGWCC	3 cut(s)	498, 749, 1835
XapI	RAATTY	4 cut(s)	673, 1025, 1211, 1577
XbaI	TCTAGA	2 cut(s)	1340, 1879
XcmI	CCANNNNNNNNNTGG	1 cut(s)	924
XmiI	GTMKAC	1 cut(s)	2264
XmnI	GAANNNNTTC	3 cut(s)	119, 888, 969
XspI	CTAG	8 cut(s)	593, 893, 1341, 1383, 1508, 1617, 1880, 2165

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.