Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005400

Physical Location & Seq

Genomic Coordinates Reverse (-)

32579880 .. 32586939

Gene Structure 7060 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ45036

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3042 bp

ATGGCTCTGGTGAAGCCCCATTCCCTCTCACCTCTCTCTTCCAACTCCCGCCATGCCCTCCCCCTCAACAACTCCCACTTCCGCACTTCTTTCTTCGCCGGAAAACCCTTCCGACTGCCGGAAACCACACGGATTTCGAGCCGGAGACGCCGGCGAGTGCAGGCAGTGGCTTCCCTGGGAGGTTTGTTGGGCGGGATTTTCAAAGGGACTGACACCGGTGAGTCCACCAGGCAGCAGTACGCTCAGACTGTGTCTCTGATCAACGGCTTGGAAGCTCAGATGTCTAAGCTTTCCGATTCCGAGCTCAGGGAGAAGACTCTTCAGTTTCAACAACGTGCCAAGCAGGGCGAGTCCTTGGACTCTCTTTTACCCGAAGCATTTGCTGTGATCAGAGAGGCTTCGAGGAGGGTTTTAGGCCTTCGTCCCTTTGACGTCCAACTCATAGGAGGCATAGTTCTTCACAAGGGGGAAATAGCTGAAATGAGGACTGGAGAAGGAAAGACCCTTGTTGCTATTTTACCAGCGTATTTGAATGCATTAACTGGAAAGGGAGTTCATGTTGTCACCGTCAATGATTATCTGGCCCGACGTGATTGTGAGTGGGTTGGTCAAGTTCCTCGTTTCCTTGGATTGAAGGTTGGCCTAATCCAACAGAATATGACAAGTGAACAAAGAAGGGAGAACTACTTATGTGACATCACATATGTCACAAACAGCGAGCTTGGTTTTGATTTCCTGAGAGATAATCTGGCCACGGAAAGTAATTCTTGCTTTATTTTCTTTACTAATAGTGTTGAGGAGCTTGTCTTGAGGAATTTCAATTACTGCGTAATTGATGAGGTTGATTCCATCCTTATTGATGAAGCAAGAACACCTCTCATTATATCTGGACCTGCAGAAAAACCTAGCGATAGGTATTATAAAGCTGCAAAGATGGCTTCAGTGTTTGAGCGAGATATACATTATACTGTGGATGAAAAGCAGAAAACAGTTCTGCTTTCAGAACAAGGATATGAAGATGCTGAAGAGATTCTAGGTGTAAAAGATTTATATGATCCCCGCGAACAGTGGGCATCTTATGTTCTGAATGCCGTGAAAGCAAAAGAACTGTTTCTTAGAGATGTAAATTATATAATTCGTGGCAAAGAGGTTCTTATCGTTGATGAGTTTACAGGACGAGTTATGCAGGGGAGGCGGTGGAGTGATGGACTTCACCAAGCAGTTGAAGCAAAAGAAGGTCTGCCGATTCAAAACGAAACTGTAACTCTGGCATCTATTAGCTACCAAAACTTCTTTCTCCAGTTTCCAAAACTTTGTGGAATGACTGGAACTGCAGCAACTGAAAGCACAGAATTTGAAAGCATATACAAGCTTAAAGTTACAATTGATGAGTCTGATGTAGTTTTCAGGGCAGCTACAGGTAAATGGCGGGCAGTTGTAGTAGAGATTTCTAGAATGCACAAAACAGGTCGCCCTGTACTTGTTGGCACAACTAGTGTTGAGCAGAGTGACTCATTGTCTGAGCAGTTGCAAGAAGTTGGCATTCCCCATGAGGTTCTCAACGCAAAACCAGAGAATGTGGAGAGGGAAGCAGAAATTGTGGCACAAAGCGGTCGTCTAGGGGCAGTTACTATTGCAACCAATATGGCAGGTCGAGGAACAGACATAATCCTTGGTGGGAATGCAGAATTTATGGCAAGGTTGAAGCTGCGTGAGATGCTTATGCCAAGAGTTGTAAAACTAACTGAAGGAGGTTATGTCTCAGTGAAAAAACTTCCGCCAAAAAAGTCCTGGAAGGTTAATGAAAAGCTATTTCCATGCAAACTGTCCAATGAAAAGACCAAGTTAGCTGAGGAAGCTGTGAATTTAGCTGTTGAAACTTGGGGTCAGAGATCATTGACGGAGCTTGAAGCAGAGGAGCGTCTTTCTTATTCTTGCGAAAAGGGACCTGCTTTGGATGAAGTGATAGCCAAACTGCGGAGTGCTTTTTTAGAAATCATGAAAGAATATAAGGGCTACACAGAGGAAGAAAGGAAGAAGGTTGTGTCAGCTGGTGGGCTTCATGTTGTCGGGACAGAACGCCATGAATCTCGCCGAGTTGATAATCAGTTACGTGGTCGAACTGGACGGCAGGGGGATCCTGGAAGTTCACGCTTCTTCTTAAGTCTTGAAGATAACATCTTTCGTATATTTGGTGGAGATCGTATTCAGGGTTTAATGAGAGCTTTTAGAGTTGAGGACTTGCCAATTGAGTCCAAGATGCTGACCAAAGCACTAGACGAAGCTCAGCGGAAAGTGGAAAACTATTTTTTCGATATTCGAAAGCAATTGTTTGAATTTGATGAAGTCCTGAACAGCCAAAGAGACCGTGTGTATACGGAAAGAAGGAGAGCCCTTGAATCTGACAATCTGCAATCACTTATAATTGAATATGCTGAACTAACAATGGATGATATCTTAGAGGCAAATATTGGTTCAGAGTCTTCAAAAGAAAGCTGGGATCTTGATAAGCTCATCAAGAAACTTCAACAATATTGCTATCTATTGAATGATTTGACGCCGGATGTGCTGAGGAGCGAATGTTCAAGTTATGAAGATTTGCAGGACTACCTCCGTCTTCGAGGTCGTGAAGCATATTTGCAGAAAAGGACTATTATCGAGAGCCAGGCACCGGGCTTGATGAAGGACGCTGAACGGTTCTTAGTTTTGAACAATATTGACCGATTGTGGAAGGAGCACTTACAAGCACTCAAGTTCGTTCAGCAAGCTGTTGGTTTGCGTGGGTACGCACAACGGGATCCACTTATTGAATATAAGCTTGAAGGCTACAATCTTTTCTTGGACATGATGGCACAGATAAGAAGAAATGTTATATATTCCATATACCAGTTTCAACCTGTGATGGTAAAGAAGGATGGGGATCAAAGAGAGAATAAGAAATCAGGAAAACTTGTCACTAATGGTAGCGGTAATGGTAACCCCAATTCAGTTGGCGGTGTTGAGTCTTCTTCAGCAGCTGCCACCCCCCAATCTAGTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071806 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW

Protein Analysis

1013

Amino Acids

114.52

Weight (kDa)

6.44

Isoelectric Point (pI)

46.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SecA_DEAD	PF07517	74 - 453	6.6e-123	SecA DEAD-like domain
DEAD	PF00270	158 - 286	1.6e-06	DEAD/DEAH box helicase
SecA_PP_bind	PF01043	303 - 409	1e-37	SecA preprotein cross-linking domain
P-loop_SecA	PF21090	462 - 747	3.2e-80	SecA P-loop domain
SecA_SW	PF07516	749 - 965	2.8e-56	SecA Wing and Scaffold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01800 AT4G01800 AT4G01800
fragaria_vesca	FvH4_1g26240
malus_domestica	MD17G1285000.v1.1
prunus_persica	Prupe.3G002700_v2.0.a1
pyrus_communis	pycom09g19850 pycom17g28080 pycom17g28090
rosa_chinensis	RchiOBHm_Chr3g0485781
rosa_laevigata	RLG00000023125
rosa_multiflora	Rmu_co8471925.1_g000001 Rmu_sc0012870.1_g000002
rosa_roxburghii	Rroxscaffold_2G00123840
rosa_rugosa	Rorug03G0221400
rosa_samantha	Rh1AG180100 Rh1CG167100 Rh3AG270700 Rh3BG306200 Rh3BG306300 Rh3CG304600 Rh3DG300700 Rh4AG241300 Rh4AG241400 Rh4BG244600 Rh4BG244700 Rh4CG255400 Rh7CG119700
rosa_wichuraiana	Rw3G024110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	921, 2423
AatII	GACGTC	1 cut(s)	435
Acc36I	ACCTGC	3 cut(s)	901, 1640, 1957
AccB1I	GGYRCC	1 cut(s)	2668
AccI	GTMKAC	1 cut(s)	2375
AccII	CGCG	1 cut(s)	1062
AclWI	GGATC	7 cut(s)	1049, 2132, 2145, 2508, 2792, 2805, 2928
AcoI	YGGCCR	1 cut(s)	750
AcsI	RAATTY	5 cut(s)	814, 1352, 1688, 1864, 2336
AcuI	CTGAAG	5 cut(s)	305, 924, 1044, 1767, 2994
AcyI	GRCGYC	3 cut(s)	148, 432, 2558
AfaI	GTAC	3 cut(s)	239, 1479, 2786
AfiI	CCNNNNNNNGG	4 cut(s)	118, 306, 1977, 2671
AflII	CTTAAG	1 cut(s)	2161
AgeI	ACCGGT	1 cut(s)	215
AhdI	GACNNNNNGTC	1 cut(s)	1516
AhlI	ACTAGT	1 cut(s)	1493
AjiI	CACGTC	1 cut(s)	590
AjnI	CCWGG	5 cut(s)	174, 227, 1790, 2140, 2664
AjuI	GAANNNNNNNTTGG	2 cut(s)	1278, 1310
AloI	GAACNNNNNNTCC	6 cut(s)	438, 470, 537, 569, 2566, 2598
Alw21I	GWGCWC	2 cut(s)	306, 2739
Alw26I	GTCTC	4 cut(s)	139, 258, 1765, 2358
AlwI	GGATC	7 cut(s)	1049, 2132, 2145, 2508, 2792, 2805, 2928
AlwNI	CAGNNNCTG	2 cut(s)	1340, 3017
AoxI	GGCC	4 cut(s)	415, 582, 640, 750
ApeKI	GCWGC	7 cut(s)	232, 926, 1334, 1412, 1708, 3014, 3017
ApoI	RAATTY	5 cut(s)	814, 1352, 1688, 1864, 2336
ArsI	GACNNNNNNTTYG	2 cut(s)	1600, 1632
AsiGI	ACCGGT	1 cut(s)	215
Asp700I	GAANNNNTTC	2 cut(s)	1029, 1110
AspS9I	GGNCC	3 cut(s)	583, 890, 1946
AsuC2I	CCSGG	1 cut(s)	2673
AsuHPI	GGTGA	5 cut(s)	21, 22, 230, 556, 1205
AsuII	TTCGAA	1 cut(s)	2320
AvaII	GGWCC	2 cut(s)	890, 1946
BalI	TGGCCA	1 cut(s)	752
BamHI	GGATCC	2 cut(s)	2137, 2797
BanI	GGYRCC	1 cut(s)	2668
BanII	GRGCYC	2 cut(s)	306, 2395
BbsI	GAAGAC	4 cut(s)	320, 2475, 2609, 2997
Bbv12I	GWGCWC	2 cut(s)	306, 2739
BbvCI	CCTCAGC	2 cut(s)	1851, 2570
BbvI	GCAGC	7 cut(s)	244, 913, 1346, 1424, 1695, 3004, 3026
BccI	CCATC	6 cut(s)	857, 928, 1199, 2842, 2896, 2909
BceAI	ACGGC	3 cut(s)	280, 1076, 2144
BcgI	CGANNNNNNTGC	2 cut(s)	362, 396
BciT130I	CCWGG	5 cut(s)	176, 229, 1792, 2142, 2666
BclI	TGATCA	2 cut(s)	258, 387
BcnI	CCSGG	1 cut(s)	2673
BcoDI	GTCTC	4 cut(s)	139, 258, 1765, 2358
BcuI	ACTAGT	1 cut(s)	1493
BfaI	CTAG	7 cut(s)	906, 1034, 1452, 1494, 1619, 2276, 3033
BfmI	CTRYAG	3 cut(s)	894, 1332, 1416
BfrI	CTTAAG	1 cut(s)	2161
BfuAI	ACCTGC	3 cut(s)	901, 1640, 1957
BisI	GCNGC	7 cut(s)	233, 927, 1335, 1413, 1709, 3015, 3018
BlpI	GCTNAGC	1 cut(s)	2286
BlsI	GCNGC	7 cut(s)	234, 928, 1336, 1414, 1710, 3016, 3019
Bme1390I	CCNGG	6 cut(s)	176, 229, 1792, 2142, 2666, 2673
Bme18I	GGWCC	2 cut(s)	890, 1946
BmeRI	GACNNNNNGTC	1 cut(s)	1516
BmgBI	CACGTC	1 cut(s)	590
BmgT120I	GGNCC	3 cut(s)	583, 890, 1946
BmiI	GGNNCC	4 cut(s)	1947, 2139, 2670, 2799
BmrFI	CCNGG	6 cut(s)	176, 229, 1792, 2142, 2666, 2673
BmsI	GCATC	5 cut(s)	1009, 1082, 1280, 1707, 2250
BpiI	GAAGAC	4 cut(s)	320, 2475, 2609, 2997
BpmI	CTGGAG	2 cut(s)	510, 1283
Bpu10I	CCTNAGC	3 cut(s)	305, 1851, 2570
Bpu1102I	GCTNAGC	1 cut(s)	2286
Bpu14I	TTCGAA	1 cut(s)	2320
BpuEI	CTTGAG	2 cut(s)	829, 2735
BpuMI	CCSGG	1 cut(s)	2673
BsaAI	YACGTR	1 cut(s)	2114
BsaBI	GATNNNNATC	1 cut(s)	2919
BsaHI	GRCGYC	3 cut(s)	148, 432, 2558
BsaI	GGTCTC	1 cut(s)	2358
BsaJI	CCNNGG	6 cut(s)	174, 175, 354, 625, 753, 1672
BsaWI	WCCGGW	1 cut(s)	215
BsaXI	ACNNNNNCTCC	8 cut(s)	438, 468, 543, 573, 1743, 1773, 2566, 2596
Bsc4I	CCNNNNNNNGG	4 cut(s)	118, 306, 1977, 2671
Bse118I	RCCGGY	2 cut(s)	150, 215
Bse1I	ACTGG	6 cut(s)	493, 547, 1300, 1330, 2128, 2887
Bse8I	GATNNNNATC	1 cut(s)	2919
BseBI	CCWGG	5 cut(s)	176, 229, 1792, 2142, 2666
BseDI	CCNNGG	6 cut(s)	174, 175, 354, 625, 753, 1672
BseGI	GGATG	6 cut(s)	849, 979, 1963, 2455, 2569, 2920
BseJI	GATNNNNATC	1 cut(s)	2919
BseLI	CCNNNNNNNGG	4 cut(s)	118, 306, 1977, 2671
BseMII	CTCAG	9 cut(s)	257, 290, 319, 728, 1512, 1776, 1842, 2300, 2561
BseNI	ACTGG	6 cut(s)	493, 547, 1300, 1330, 2128, 2887
BseRI	GAGGAG	4 cut(s)	418, 812, 1931, 2587
BseXI	GCAGC	7 cut(s)	244, 913, 1346, 1424, 1695, 3004, 3026
BseYI	CCCAGC	1 cut(s)	2496
BsgI	GTGCAG	1 cut(s)	179
Bsh1236I	CGCG	1 cut(s)	1062
Bsh1285I	CGRYCG	1 cut(s)	1615
BshFI	GGCC	4 cut(s)	417, 584, 642, 752
BshNI	GGYRCC	1 cut(s)	2668
BshTI	ACCGGT	1 cut(s)	215
BsiEI	CGRYCG	1 cut(s)	1615
BsiHKAI	GWGCWC	2 cut(s)	306, 2739
BsiSI	CCGG	7 cut(s)	99, 119, 142, 151, 216, 2561, 2672
BslFI	GGGAC	4 cut(s)	220, 408, 1959, 2086
BslI	CCNNNNNNNGG	4 cut(s)	118, 306, 1977, 2671
BsmAI	GTCTC	4 cut(s)	139, 258, 1765, 2358
BsmBI	CGTCTC	1 cut(s)	139
BsmFI	GGGAC	4 cut(s)	220, 408, 1959, 2086
BsmI	GAATGC	5 cut(s)	538, 1093, 1461, 1542, 1687
BsnI	GGCC	4 cut(s)	417, 584, 642, 752
Bso31I	GGTCTC	1 cut(s)	2358
Bsp119I	TTCGAA	1 cut(s)	2320
Bsp1286I	GDGCHC	3 cut(s)	306, 2395, 2739
Bsp143I	GATC	9 cut(s)	258, 387, 1054, 1892, 2137, 2200, 2500, 2797, 2920
Bsp1720I	GCTNAGC	1 cut(s)	2286
BspANI	GGCC	4 cut(s)	417, 584, 642, 752
BspCNI	CTCAG	9 cut(s)	256, 289, 318, 729, 1513, 1775, 1843, 2299, 2562
BspFNI	CGCG	1 cut(s)	1062
BspHI	TCATGA	1 cut(s)	1998
BspLI	GGNNCC	4 cut(s)	1947, 2139, 2670, 2799
BspMAI	CTGCAG	2 cut(s)	898, 1336
BspMI	ACCTGC	3 cut(s)	901, 1640, 1957
BspPI	GGATC	7 cut(s)	1049, 2132, 2145, 2508, 2792, 2805, 2928
BspT104I	TTCGAA	1 cut(s)	2320
BspT107I	GGYRCC	1 cut(s)	2668
BspTI	CTTAAG	1 cut(s)	2161
BspTNI	GGTCTC	1 cut(s)	2358
BsrFI	RCCGGY	2 cut(s)	150, 215
BsrI	ACTGG	6 cut(s)	493, 547, 1300, 1330, 2128, 2887
BssAI	RCCGGY	2 cut(s)	150, 215
BssECI	CCNNGG	6 cut(s)	174, 175, 354, 625, 753, 1672
BssMI	GATC	9 cut(s)	258, 387, 1054, 1892, 2137, 2200, 2500, 2797, 2920
BssNAI	GTATAC	1 cut(s)	2376
BssNI	GRCGYC	3 cut(s)	148, 432, 2558
BssT1I	CCWWGG	3 cut(s)	354, 625, 1672
Bst1107I	GTATAC	1 cut(s)	2376
Bst2UI	CCWGG	5 cut(s)	176, 229, 1792, 2142, 2666
Bst6I	CTCTTC	3 cut(s)	43, 324, 1020
BstACI	GRCGYC	3 cut(s)	148, 432, 2558
BstAFI	CTTAAG	1 cut(s)	2161
BstBAI	YACGTR	1 cut(s)	2114
BstBI	TTCGAA	1 cut(s)	2320
BstC8I	GCNNGC	5 cut(s)	152, 162, 719, 1431, 2766
BstDSI	CCRYGG	1 cut(s)	753
BstEII	GGTNACC	1 cut(s)	2975
BstF5I	GGATG	6 cut(s)	849, 979, 1963, 2455, 2569, 2920
BstFNI	CGCG	1 cut(s)	1062
BstKTI	GATC	9 cut(s)	261, 390, 1057, 1895, 2140, 2203, 2503, 2800, 2923
BstMAI	GTCTC	4 cut(s)	139, 258, 1765, 2358
BstMBI	GATC	9 cut(s)	258, 387, 1054, 1892, 2137, 2200, 2500, 2797, 2920
BstMCI	CGRYCG	1 cut(s)	1615
BstMWI	GCNNNNNNNGC	8 cut(s)	147, 935, 1097, 1192, 1226, 1717, 1856, 2566
BstNI	CCWGG	5 cut(s)	176, 229, 1792, 2142, 2666
BstPI	GGTNACC	1 cut(s)	2975
BstSCI	CCNGG	6 cut(s)	174, 227, 1790, 2140, 2664, 2671
BstSFI	CTRYAG	3 cut(s)	894, 1332, 1416
BstUI	CGCG	1 cut(s)	1062
BstV1I	GCAGC	7 cut(s)	244, 913, 1346, 1424, 1695, 3004, 3026
BstV2I	GAAGAC	4 cut(s)	320, 2475, 2609, 2997
BstX2I	RGATCY	3 cut(s)	2137, 2500, 2797
BstYI	RGATCY	3 cut(s)	2137, 2500, 2797
BstZ17I	GTATAC	1 cut(s)	2376
BsuRI	GGCC	4 cut(s)	417, 584, 642, 752
BtgI	CCRYGG	1 cut(s)	753
BtrI	CACGTC	1 cut(s)	590
BtsCI	GGATG	6 cut(s)	849, 979, 1963, 2455, 2569, 2920
BtsI	GCAGTG	1 cut(s)	171
BtsIMutI	CAGTG	4 cut(s)	171, 948, 1073, 1770
BveI	ACCTGC	3 cut(s)	901, 1640, 1957
Cac8I	GCNNGC	5 cut(s)	152, 162, 719, 1431, 2766
CaiI	CAGNNNCTG	2 cut(s)	1340, 3017
CciI	TCATGA	1 cut(s)	1998
Cfr10I	RCCGGY	2 cut(s)	150, 215
Cfr13I	GGNCC	3 cut(s)	583, 890, 1946
CseI	GACGC	4 cut(s)	156, 1910, 2566, 2696
Csp6I	GTAC	3 cut(s)	238, 1478, 2785
CspAI	ACCGGT	1 cut(s)	215
CviAII	CATG	8 cut(s)	53, 557, 1550, 1818, 1999, 2063, 2084, 2845
CviQI	GTAC	3 cut(s)	238, 1478, 2785
DpnI	GATC	9 cut(s)	260, 389, 1056, 1894, 2139, 2202, 2502, 2799, 2922
DpnII	GATC	9 cut(s)	258, 387, 1054, 1892, 2137, 2200, 2500, 2797, 2920
DriI	GACNNNNNGTC	1 cut(s)	1516
EaeI	YGGCCR	1 cut(s)	750
Eam1104I	CTCTTC	3 cut(s)	43, 324, 1020
Eam1105I	GACNNNNNGTC	1 cut(s)	1516
EarI	CTCTTC	3 cut(s)	43, 324, 1020
EciI	GGCGGA	1 cut(s)	1767
Ecl136II	GAGCTC	1 cut(s)	304
Eco130I	CCWWGG	3 cut(s)	354, 625, 1672
Eco147I	AGGCCT	1 cut(s)	417
Eco24I	GRGCYC	2 cut(s)	306, 2395
Eco31I	GGTCTC	1 cut(s)	2358
Eco32I	GATATC	1 cut(s)	2455
Eco47I	GGWCC	2 cut(s)	890, 1946
Eco53kI	GAGCTC	1 cut(s)	304
Eco57I	CTGAAG	5 cut(s)	305, 924, 1044, 1767, 2994
Eco91I	GGTNACC	1 cut(s)	2975
EcoICRI	GAGCTC	1 cut(s)	304
EcoO109I	RGGNCCY	1 cut(s)	1946
EcoO65I	GGTNACC	1 cut(s)	2975
EcoRII	CCWGG	5 cut(s)	174, 227, 1790, 2140, 2664
EcoRV	GATATC	1 cut(s)	2455
EcoT14I	CCWWGG	3 cut(s)	354, 625, 1672
EcoT22I	ATGCAT	1 cut(s)	538
EcoT38I	GRGCYC	2 cut(s)	306, 2395
ErhI	CCWWGG	3 cut(s)	354, 625, 1672
Esp3I	CGTCTC	1 cut(s)	139
FaeI	CATG	8 cut(s)	56, 560, 1553, 1821, 2002, 2066, 2087, 2848
FalI	AAGNNNNNCTT	6 cut(s)	751, 783, 1137, 1169, 2723, 2755
FaqI	GGGAC	4 cut(s)	220, 408, 1959, 2086
FatI	CATG	8 cut(s)	52, 556, 1549, 1817, 1998, 2062, 2083, 2844
FauI	CCCGC	4 cut(s)	56, 185, 1067, 1422
FauNDI	CATATG	1 cut(s)	703
FbaI	TGATCA	2 cut(s)	258, 387
FblI	GTMKAC	1 cut(s)	2375
Fnu4HI	GCNGC	7 cut(s)	233, 927, 1335, 1413, 1709, 3015, 3018
FokI	GGATG	6 cut(s)	836, 986, 1970, 2462, 2576, 2927
FriOI	GRGCYC	2 cut(s)	306, 2395
Fsp4HI	GCNGC	7 cut(s)	233, 927, 1335, 1413, 1709, 3015, 3018
FspBI	CTAG	7 cut(s)	906, 1034, 1452, 1494, 1619, 2276, 3033
GluI	GCNGC	7 cut(s)	233, 927, 1335, 1413, 1709, 3015, 3018
GsaI	CCCAGC	1 cut(s)	2500
GsuI	CTGGAG	2 cut(s)	510, 1283
HaeIII	GGCC	4 cut(s)	417, 584, 642, 752
HapII	CCGG	7 cut(s)	99, 119, 142, 151, 216, 2561, 2672
HgaI	GACGC	4 cut(s)	156, 1910, 2566, 2696
Hin1I	GRCGYC	3 cut(s)	148, 432, 2558
Hin1II	CATG	8 cut(s)	56, 560, 1553, 1821, 2002, 2066, 2087, 2848
HindIII	AAGCTT	3 cut(s)	287, 1370, 2816
HpaII	CCGG	7 cut(s)	99, 119, 142, 151, 216, 2561, 2672
HphI	GGTGA	5 cut(s)	21, 22, 230, 556, 1205
Hpy166II	GTNNAC	5 cut(s)	225, 668, 1170, 2150, 2376
Hpy8I	GTNNAC	5 cut(s)	225, 668, 1170, 2150, 2376
Hpy99I	CGWCG	1 cut(s)	591
HpyCH4IV	ACGT	4 cut(s)	334, 432, 589, 2113
HpyF10VI	GCNNNNNNNGC	8 cut(s)	147, 935, 1097, 1192, 1226, 1717, 1856, 2566
HpySE526I	ACGT	4 cut(s)	334, 432, 589, 2113
Hsp92I	GRCGYC	3 cut(s)	148, 432, 2558
Hsp92II	CATG	8 cut(s)	56, 560, 1553, 1821, 2002, 2066, 2087, 2848
KroI	GCCGGC	1 cut(s)	150
KroNI	GCCGGC	1 cut(s)	152
Ksp22I	TGATCA	2 cut(s)	258, 387
Kzo9I	GATC	9 cut(s)	258, 387, 1054, 1892, 2137, 2200, 2500, 2797, 2920
LmnI	GCTCC	5 cut(s)	799, 1903, 1918, 2574, 2734
Lsp1109I	GCAGC	7 cut(s)	244, 913, 1346, 1424, 1695, 3004, 3026
LweI	GCATC	5 cut(s)	1009, 1082, 1280, 1707, 2250
MaeI	CTAG	7 cut(s)	906, 1034, 1452, 1494, 1619, 2276, 3033
MaeII	ACGT	4 cut(s)	334, 432, 589, 2113
MalI	GATC	9 cut(s)	260, 389, 1056, 1894, 2139, 2202, 2502, 2799, 2922
MboI	GATC	9 cut(s)	258, 387, 1054, 1892, 2137, 2200, 2500, 2797, 2920
MfeI	CAATTG	3 cut(s)	1383, 2247, 2327
MflI	RGATCY	3 cut(s)	2137, 2500, 2797
MhlI	GDGCHC	3 cut(s)	306, 2395, 2739
MlsI	TGGCCA	1 cut(s)	752
MluNI	TGGCCA	1 cut(s)	752
MlyI	GAGTC	9 cut(s)	230, 310, 353, 359, 1400, 1505, 2261, 2489, 3011
MmeI	TCCRAC	4 cut(s)	66, 136, 460, 673
Mox20I	TGGCCA	1 cut(s)	752
Mph1103I	ATGCAT	1 cut(s)	538
MreI	CGCCGGCG	1 cut(s)	150
MroNI	GCCGGC	1 cut(s)	150
MroXI	GAANNNNTTC	2 cut(s)	1029, 1110
MscI	TGGCCA	1 cut(s)	752
MseI	TTAA	5 cut(s)	539, 1374, 1800, 2162, 2216
Msp20I	TGGCCA	1 cut(s)	752
MspA1I	CMGCKG	3 cut(s)	2051, 2290, 3017
MspCI	CTTAAG	1 cut(s)	2161
MspI	CCGG	7 cut(s)	99, 119, 142, 151, 216, 2561, 2672
MspR9I	CCNGG	6 cut(s)	176, 229, 1792, 2142, 2666, 2673
MunI	CAATTG	3 cut(s)	1383, 2247, 2327
Mva1269I	GAATGC	5 cut(s)	538, 1093, 1461, 1542, 1687
MvaI	CCWGG	5 cut(s)	176, 229, 1792, 2142, 2666
MvnI	CGCG	1 cut(s)	1062
MwoI	GCNNNNNNNGC	8 cut(s)	147, 935, 1097, 1192, 1226, 1717, 1856, 2566
NaeI	GCCGGC	1 cut(s)	152
NciI	CCSGG	1 cut(s)	2673
NdeI	CATATG	1 cut(s)	703
NdeII	GATC	9 cut(s)	258, 387, 1054, 1892, 2137, 2200, 2500, 2797, 2920
NgoMIV	GCCGGC	1 cut(s)	150
NlaIII	CATG	8 cut(s)	56, 560, 1553, 1821, 2002, 2066, 2087, 2848
NlaIV	GGNNCC	4 cut(s)	1947, 2139, 2670, 2799
NmeAIII	GCCGAG	1 cut(s)	2120
NmuCI	GTSAC	5 cut(s)	562, 692, 706, 1508, 2953
NsiI	ATGCAT	1 cut(s)	538
NspV	TTCGAA	1 cut(s)	2320
PagI	TCATGA	1 cut(s)	1998
PasI	CCCWGGG	1 cut(s)	175
PceI	AGGCCT	1 cut(s)	417
PctI	GAATGC	5 cut(s)	538, 1093, 1461, 1542, 1687
PdiI	GCCGGC	1 cut(s)	152
PdmI	GAANNNNTTC	2 cut(s)	1029, 1110
PfeI	GAWTC	6 cut(s)	296, 845, 1030, 1246, 2087, 2399
PflFI	GACNNNGTC	1 cut(s)	250
PfoI	TCCNGGA	2 cut(s)	1790, 2140
PinAI	ACCGGT	1 cut(s)	215
PkrI	GCNGC	7 cut(s)	234, 928, 1336, 1414, 1710, 3016, 3019
PleI	GAGTC	9 cut(s)	229, 310, 353, 358, 1399, 1505, 2260, 2488, 3010
PpsI	GAGTC	9 cut(s)	229, 310, 353, 358, 1399, 1505, 2260, 2488, 3010
Ppu21I	YACGTR	1 cut(s)	2114
PpuMI	RGGWCCY	1 cut(s)	1946
PsiI	TTATAA	2 cut(s)	921, 2423
Psp124BI	GAGCTC	1 cut(s)	306
Psp5II	RGGWCCY	1 cut(s)	1946
Psp6I	CCWGG	5 cut(s)	174, 227, 1790, 2140, 2664
PspEI	GGTNACC	1 cut(s)	2975
PspFI	CCCAGC	1 cut(s)	2496
PspGI	CCWGG	5 cut(s)	174, 227, 1790, 2140, 2664
PspN4I	GGNNCC	4 cut(s)	1947, 2139, 2670, 2799
PspPI	GGNCC	3 cut(s)	583, 890, 1946
PspPPI	RGGWCCY	1 cut(s)	1946
PstI	CTGCAG	2 cut(s)	898, 1336
PstNI	CAGNNNCTG	2 cut(s)	1340, 3017
PsuI	RGATCY	3 cut(s)	2137, 2500, 2797
PsyI	GACNNNGTC	1 cut(s)	250
PvuII	CAGCTG	2 cut(s)	2051, 3017
RsaI	GTAC	3 cut(s)	239, 1479, 2786
RsaNI	GTAC	3 cut(s)	238, 1478, 2785
SacI	GAGCTC	1 cut(s)	306
SaqAI	TTAA	5 cut(s)	539, 1374, 1800, 2162, 2216
SatI	GCNGC	7 cut(s)	233, 927, 1335, 1413, 1709, 3015, 3018
Sau3AI	GATC	9 cut(s)	258, 387, 1054, 1892, 2137, 2200, 2500, 2797, 2920
Sau96I	GGNCC	3 cut(s)	583, 890, 1946
SchI	GAGTC	9 cut(s)	230, 310, 353, 359, 1400, 1505, 2261, 2489, 3011
ScrFI	CCNGG	6 cut(s)	176, 229, 1792, 2142, 2666, 2673
SduI	GDGCHC	3 cut(s)	306, 2395, 2739
SfaNI	GCATC	5 cut(s)	1009, 1082, 1280, 1707, 2250
SfcI	CTRYAG	3 cut(s)	894, 1332, 1416
SfuI	TTCGAA	1 cut(s)	2320
SgrAI	CRCCGGYG	2 cut(s)	150, 215
SinI	GGWCC	2 cut(s)	890, 1946
SmlI	CTYRAG	3 cut(s)	808, 2161, 2750
SmoI	CTYRAG	3 cut(s)	808, 2161, 2750
SpeI	ACTAGT	1 cut(s)	1493
SseBI	AGGCCT	1 cut(s)	417
SspI	AATATT	3 cut(s)	2470, 2534, 2716
SspMI	CTAG	7 cut(s)	906, 1034, 1452, 1494, 1619, 2276, 3033
SstI	GAGCTC	1 cut(s)	306
StuI	AGGCCT	1 cut(s)	417
StyD4I	CCNGG	6 cut(s)	174, 227, 1790, 2140, 2664, 2671
StyI	CCWWGG	3 cut(s)	354, 625, 1672
TaiI	ACGT	4 cut(s)	337, 435, 592, 2116
TaqI	TCGA	8 cut(s)	137, 401, 1654, 2119, 2313, 2320, 2620, 2658
TaqII	GACCGA	1 cut(s)	2736
TatI	WGTACW	1 cut(s)	1477
TfiI	GAWTC	6 cut(s)	296, 845, 1030, 1246, 2087, 2399
Tru1I	TTAA	5 cut(s)	539, 1374, 1800, 2162, 2216
Tru9I	TTAA	5 cut(s)	539, 1374, 1800, 2162, 2216
TscAI	CASTG	4 cut(s)	171, 948, 1073, 1770
TseFI	GTSAC	5 cut(s)	562, 692, 706, 1508, 2953
TseI	GCWGC	7 cut(s)	232, 926, 1334, 1412, 1708, 3014, 3017
Tsp45I	GTSAC	5 cut(s)	562, 692, 706, 1508, 2953
TspGWI	ACGGA	5 cut(s)	145, 770, 1916, 2393, 2603
TspRI	CASTG	4 cut(s)	171, 948, 1073, 1770
Tth111I	GACNNNGTC	1 cut(s)	250
Vha464I	CTTAAG	1 cut(s)	2161
VpaK11BI	GGWCC	2 cut(s)	890, 1946
XapI	RAATTY	5 cut(s)	814, 1352, 1688, 1864, 2336
XbaI	TCTAGA	1 cut(s)	1451
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1788
XmiI	GTMKAC	1 cut(s)	2375
XmnI	GAANNNNTTC	2 cut(s)	1029, 1110
XspI	CTAG	7 cut(s)	906, 1034, 1452, 1494, 1619, 2276, 3033
ZraI	GACGTC	1 cut(s)	433
Zsp2I	ATGCAT	1 cut(s)	538

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.