Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0005400

Physical Location & Seq

Genomic Coordinates Reverse (-)

20193058 .. 20196707

Gene Structure 3650 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0221400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3099 bp

ATGCGAGGCTTATCCGCCAAATTACAGTCCTTGTGTTGGAGCTCAACCTCTAAAACCCTTCATTTCCGATTGTCTAAATTTCAAAAGATTGATAACTTTGCGACGCTTACTAAGCCATCTAAGCCCAACAAAGCCCACTTGAAACCACAACAAGAAGAACCCAGCTCGTTTGGATCGTTGTTCAGTGAAATAACTGGGATTTTAGGGGCTGAAAATGTCACTGCGGATAAAACTCCATCTGGGTTTTTTATTTCCAAGGAAACCCTTCTTGGGGTTGGTGAGGTTGGGGAAGAGAGGACACCTTGCACCGCAACTCTGTGTAGAAATCCCAAGGTTGACGCACTGGAAGAAAAGGAAGATGTAGCTGAAACTGATGTTAGCCCAGTAGTTCATGAGGTTACGAAGATTGTCAGGGCGGAAAATGGTTTGGTTTCGATGGAGGAGCGGTTAGACGAGTTGGGTTTTCAGTTAGATTCAGAGATTGTTGACAAAGTTCTGAAGAGGTGCTTTAAAGTGCCGCATTTGGCTTTAAGGTTCTTTGATTGGGTGAAACTGAAAGAAGGGTTCTGTCACACGACTAAGACTTATAATACCGTGCTGTGTATAGCTGGGGAGGCAAGAGAGTTTGGGCTGGTGGAGAAGTTGGTGGAGGAGATGGAAAGGAACTCGTGTGAGAAAGATGTTAAGACCTGGACCATTCTTATCCAGATGTATGGGAAGGCTAAGTTAGTTGGAAGAGCGTTGTCAGTCTATGAGAAGATGAAGAAATGCGGTTTTGAACCAGACTCGGTGGTTTTCAGAATGATGATACGTTCACTTTGTGCTGCTGGAAAAGCCGACGTGGCAATGGAATTTTACAAGGAAATGGTACAGAAGGACATTGGACTTGATATCAATATGTACAAGCTGTTATTGAATGGCATAGCTAGCTACGGAGATACTGCTGCTGTGGGCTTGGTTTCAGATGACATGATGAGAGTCTCACAGATTCCTGAACATGTGGTTTATGGTTGTGTCCTGAAGTCTTTCTGTATCTCTGGTAGAATCAGAGAAGCTTTGGAATATATTCGTGAGCTCAAGAATAAAGATGTTATGCTTCTCCCTGAATATTTTGAGACTTTGGTGAAAGGACTGTGCAGGGCTGATAGAATTGCAGATGCTTTGGAGATTTTGGATATTATGAAGAAAAGAGATTTTGTTGATGGAAATGTGTATGGGATTATCATCAATGGATACTTGAGAAAAAACGATGTTTCTAGAGCACTTGATCTGTTTCGTAGTATGAAAGAATGTGGATATTTGCCTACTACTTCTACCTACACTGAACTGATGCAGCACCTCTTCAATGAGAATGAGTATCAACAAGGCTGTGAGCTGTACGATGAGATGCTTGAAAGAGGAGTTGAGCCAGATAGTGTGGCCGTTACAGCCATGGTTGCGGGCCACGTCCGCCAAAACCATATATCTGAAGCATGGAAAGTATTTAATGATATGAAGGACAAAGGCATTAAGCCCACTTCAAAGTTGTATTTAGTATTTATCAAGGAGCTTTGTAGAATTTCAAGGACAGATGAGATTCTGAAGGTCTTGGTTCATATGCGGGCATCTGATATGCGCATTAAAGATGACATATTCAATTGGGTTGTACTTCATATGGAGAAAGGGGGAGAGATGGATAGCCTTGAAAAAGTAAAGCAGATGCAAAGTCTTTATAAATTGCAGCCTCATGATGGAGAGGTATCAAGTAGTGATGCATCTAGTGGAGAGAGGACCAATACAGTGTTGGAGTTGCTACCAAAGAAGATGGATCCCTTATTGAATTCTGATGCTTATGATGACAAGGATTTGCAAGAAATTTGTAAAATTCTGTCCTCTTCAACTGACTGGTGCTTAATTCAAGAAGATTTGGAGAAGTCTGCTGTTGATTACACTCCAGGACTCGTTCTGGAAATTTTGAGGAATCACAGTGGGCATGGTTCGGTAGCATTACAGTTTTTCTCATGGGTAGGAAAGCAATCTGGTTATTGCCACACTACTGATACTTACAACATGGCTATCAAAATTGCAGGGCGTGGGAAAGATTTCAAGCACATGAGGAACCTTTTCTATGAAATGAGGAGAAACAGTTTCTCAATAACACAAGATACATGGACAATAATGATAATGCAATATGGTCGAACAGGTCTCACAGAGATTGCTCTTCAGTTTTTCAGAGAGATGAAAAGTAATTGCAACCCAACTGGCAGTACCTATAAGTATTTGATCATATCTCTTTGTGGGAGAAAAGGTCGAAGGGTAGATGAAGCAATTAAAATATTCCAGGAGATGATCCGTGCTGCACATATACCTGACAAAGAGTTGGTTGAAACTTACGTTGGTTGTTTATGTGAAGTTGGTAAGCTGTCAGATGCTAGAAGATGCATAGATTCTCTTTCCAAAGTTGGTTTTACCACCTCTCTTGGTTGTTCCTTGTACATTAGGGCTCTTTGTCGAGCTGGGAAGTTACAAGAAGCTTCTGCATTGATGGATGATATTGGGAAAGAGCGATCCACATTGGATCGGTATACTTATGGGAGCTTCGTTCATGGACTTTTGCGGAGTGGACAAGTAGAAGAAGCACTGGCCAAGGTGGCGTCTATGAAGCAGGCTGGCATGAAGCCAACCGTTCATGTTTATACATCCTTAATAGTTCATTTCTTTAAGGAGAAGCAGATCAAAAGGGCATTAGAAATTTTCAATGAAATGCAACAGGAAGGATGTGAACCGACGGTTGTTACTTATTCCGCGCTTATCCGAGGGTACATGAACATGGAGATGATTGCTGAAGCTTGGAGTGTCTTTCACAACATGAAACTGAAAGGACCATTGCCGGATTTCAAAACTTATTCAATGTTCCTTCATTGTCTTTGTAAGGCAGGGAAATCAGAAGAAGCCATGCATCTTATATCTGAAATGTTGAACAGTGGGATTGTTCCCAGTGTGGTCAATTTTCGAACTGTATTTTACGGACTTAACCGAGAAGGTAAGCAAGATTTGGCTCGCATTGTAATGGAACAGAAGTCATCTTTGATTAGAAAACGTAAGTTCTTAATATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071806 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW

Protein Analysis

1032

Amino Acids

117.14

Weight (kDa)

7.45

Isoelectric Point (pI)

27.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	193 - 239	2.6e-06	PPR repeat family
PPR_2	PF13041	226 - 274	6.7e-12	PPR repeat family
PPR	PF01535	230 - 259	4.3e-06	PPR repeat
PPR_1	PF12854	258 - 290	1.4e-06	PPR repeat
PPR_2	PF13041	261 - 309	3.4e-06	PPR repeat family
PPR_2	PF13041	405 - 444	1.9e-08	PPR repeat family
PPR_long	PF17177	413 - 543	3.3e-08	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	435 - 481	2.4e-09	PPR repeat family
PPR_2	PF13041	470 - 519	3.3e-12	PPR repeat family
PPR_2	PF13041	716 - 759	1.2e-06	PPR repeat family
TPR_24	PF23276	831 - 949	1.3e-07	Fungal tetratrico peptide repeats
PPR_long	PF17177	867 - 994	4.4e-10	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	879 - 934	2.7e-13	Pentatricopeptide repeat domain
PPR_2	PF13041	887 - 935	3.3e-15	PPR repeat family
TPR_24	PF23276	890 - 981	1.2e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	919 - 951	7.3e-06	PPR repeat
PPR_2	PF13041	922 - 971	1.6e-13	PPR repeat family
PPR_1	PF12854	953 - 985	1.8e-06	PPR repeat
PPR_2	PF13041	961 - 1004	2.7e-07	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01800 AT4G01800 AT4G01800
fragaria_vesca	FvH4_1g26240
malus_domestica	MD17G1285000.v1.1
prunus_persica	Prupe.3G002700_v2.0.a1
pyrus_communis	pycom09g19850 pycom17g28080 pycom17g28090
rosa_chinensis	RchiOBHm_Chr3g0485781
rosa_laevigata	RLG00000023125
rosa_multiflora	Rmu_co8471925.1_g000001 Rmu_sc0012870.1_g000002
rosa_roxburghii	Rroxscaffold_2G00123840
rosa_rugosa	Rorug03G0221400
rosa_samantha	Rh1AG180100 Rh1CG167100 Rh3AG270700 Rh3BG306200 Rh3BG306300 Rh3CG304600 Rh3DG300700 Rh4AG241300 Rh4AG241400 Rh4BG244600 Rh4BG244700 Rh4CG255400 Rh7CG119700
rosa_wichuraiana	Rw3G024110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	588, 1713
Acc16I	TGCGCA	1 cut(s)	1616
AccBSI	CCGCTC	1 cut(s)	445
AccI	GTMKAC	1 cut(s)	2564
AccII	CGCG	1 cut(s)	2786
AclWI	GGATC	6 cut(s)	181, 1802, 1815, 2323, 2541, 2565
AcoI	YGGCCR	2 cut(s)	1419, 2622
AcsI	RAATTY	8 cut(s)	77, 851, 1557, 1819, 1854, 1863, 1950, 2730
AcuI	CTGAAG	6 cut(s)	518, 1040, 1488, 1601, 2186, 2844
AcyI	GRCGYC	1 cut(s)	2633
AdeI	CACNNNGTG	1 cut(s)	821
AfaI	GTAC	7 cut(s)	870, 902, 1379, 1647, 2248, 2474, 2801
AfiI	CCNNNNNNNGG	4 cut(s)	36, 270, 271, 1730
AflIII	ACRYGT	1 cut(s)	997
AjiI	CACGTC	2 cut(s)	841, 1447
AjnI	CCWGG	3 cut(s)	689, 1933, 2319
AjuI	GAANNNNNNNTTGG	4 cut(s)	252, 284, 2358, 2390
Alw21I	GWGCWC	3 cut(s)	44, 1077, 1264
Alw26I	GTCTC	3 cut(s)	985, 1109, 2189
AlwI	GGATC	6 cut(s)	181, 1802, 1815, 2323, 2541, 2565
AoxI	GGCC	3 cut(s)	1419, 1441, 2622
ApeKI	GCWGC	5 cut(s)	824, 944, 1333, 1720, 2336
ApoI	RAATTY	8 cut(s)	77, 851, 1557, 1819, 1854, 1863, 1950, 2730
Asp700I	GAANNNNTTC	5 cut(s)	264, 1025, 1065, 2081, 2102
AspLEI	GCGC	2 cut(s)	1617, 2788
AspS9I	GGNCC	4 cut(s)	693, 1441, 1770, 2861
AsuHPI	GGTGA	3 cut(s)	290, 559, 1135
AsuII	TTCGAA	1 cut(s)	2992
AsuNHI	GCTAGC	1 cut(s)	926
AvaII	GGWCC	3 cut(s)	693, 1770, 2861
BalI	TGGCCA	1 cut(s)	2624
BamHI	GGATCC	1 cut(s)	1807
BanII	GRGCYC	3 cut(s)	44, 1077, 2485
BauI	CACGAG	1 cut(s)	667
Bbv12I	GWGCWC	3 cut(s)	44, 1077, 1264
BbvI	GCAGC	5 cut(s)	811, 931, 1345, 1732, 2323
BccI	CCATC	9 cut(s)	124, 244, 430, 649, 1196, 1666, 1724, 1798, 2518
BceAI	ACGGC	1 cut(s)	1406
BcgI	CGANNNNNNTGC	2 cut(s)	2156, 2190
BciT130I	CCWGG	3 cut(s)	691, 1935, 2321
BciVI	GTATCC	1 cut(s)	1226
BclI	TGATCA	1 cut(s)	2262
BcoDI	GTCTC	3 cut(s)	985, 1109, 2189
BfaI	CTAG	4 cut(s)	927, 1257, 1758, 2412
BfuI	GTATCC	1 cut(s)	1226
BglI	GCCNNNNNGGC	2 cut(s)	842, 2630
BisI	GCNGC	6 cut(s)	518, 825, 945, 1334, 1721, 2337
BlsI	GCNGC	6 cut(s)	519, 826, 946, 1335, 1722, 2338
Bme1390I	CCNGG	3 cut(s)	691, 1935, 2321
Bme18I	GGWCC	3 cut(s)	693, 1770, 2861
BmgBI	CACGTC	2 cut(s)	841, 1447
BmgT120I	GGNCC	4 cut(s)	693, 1441, 1770, 2861
BmiI	GGNNCC	2 cut(s)	1809, 2099
BmrFI	CCNGG	3 cut(s)	691, 1935, 2321
BmrI	ACTGGG	3 cut(s)	204, 377, 2970
BmtI	GCTAGC	1 cut(s)	930
BmuI	ACTGGG	3 cut(s)	204, 377, 2970
BpmI	CTGGAG	1 cut(s)	1917
Bpu14I	TTCGAA	1 cut(s)	2992
BpuEI	CTTGAG	2 cut(s)	1061, 1258
BsaBI	GATNNNNATC	1 cut(s)	1223
BsaHI	GRCGYC	1 cut(s)	2633
BsaI	GGTCTC	1 cut(s)	2189
BsaJI	CCNNGG	5 cut(s)	255, 330, 1431, 2625, 2794
BsaXI	ACNNNNNCTCC	6 cut(s)	431, 461, 2110, 2140, 2272, 2302
Bsc4I	CCNNNNNNNGG	4 cut(s)	36, 270, 271, 1730
Bse1I	ACTGG	7 cut(s)	199, 348, 383, 1889, 2245, 2625, 2976
Bse3DI	GCAATG	2 cut(s)	852, 2864
Bse8I	GATNNNNATC	1 cut(s)	1223
BseBI	CCWGG	3 cut(s)	691, 1935, 2321
BseDI	CCNNGG	5 cut(s)	255, 330, 1431, 2625, 2794
BseGI	GGATG	3 cut(s)	2533, 2678, 2762
BseJI	GATNNNNATC	1 cut(s)	1223
BseLI	CCNNNNNNNGG	4 cut(s)	36, 270, 271, 1730
BseMI	GCAATG	2 cut(s)	852, 2864
BseNI	ACTGG	7 cut(s)	199, 348, 383, 1889, 2245, 2625, 2976
BseRI	GAGGAG	4 cut(s)	455, 665, 1413, 2131
BseXI	GCAGC	5 cut(s)	811, 931, 1345, 1732, 2323
BseYI	CCCAGC	3 cut(s)	161, 608, 2495
BsgI	GTGCAG	2 cut(s)	1156, 2322
Bsh1236I	CGCG	1 cut(s)	2786
BshFI	GGCC	3 cut(s)	1421, 1443, 2624
BsiHKAI	GWGCWC	3 cut(s)	44, 1077, 1264
BsiSI	CCGG	1 cut(s)	2870
BslI	CCNNNNNNNGG	4 cut(s)	36, 270, 271, 1730
BsmAI	GTCTC	3 cut(s)	985, 1109, 2189
BsnI	GGCC	3 cut(s)	1421, 1443, 2624
Bso31I	GGTCTC	1 cut(s)	2189
Bsp119I	TTCGAA	1 cut(s)	2992
Bsp1286I	GDGCHC	4 cut(s)	44, 1077, 1264, 2485
Bsp1407I	TGTACA	2 cut(s)	900, 2472
Bsp143I	GATC	8 cut(s)	173, 1267, 1807, 2262, 2328, 2546, 2557, 2712
Bsp19I	CCATGG	1 cut(s)	1431
BspANI	GGCC	3 cut(s)	1421, 1443, 2624
BspFNI	CGCG	1 cut(s)	2786
BspHI	TCATGA	3 cut(s)	391, 1726, 3095
BspLI	GGNNCC	2 cut(s)	1809, 2099
BspOI	GCTAGC	1 cut(s)	930
BspPI	GGATC	6 cut(s)	181, 1802, 1815, 2323, 2541, 2565
BspQI	GCTCTTC	2 cut(s)	730, 2205
BspT104I	TTCGAA	1 cut(s)	2992
BspTNI	GGTCTC	1 cut(s)	2189
BsrBI	CCGCTC	1 cut(s)	445
BsrDI	GCAATG	2 cut(s)	852, 2864
BsrGI	TGTACA	2 cut(s)	900, 2472
BsrI	ACTGG	7 cut(s)	199, 348, 383, 1889, 2245, 2625, 2976
BssECI	CCNNGG	5 cut(s)	255, 330, 1431, 2625, 2794
BssMI	GATC	8 cut(s)	173, 1267, 1807, 2262, 2328, 2546, 2557, 2712
BssNAI	GTATAC	1 cut(s)	2565
BssNI	GRCGYC	1 cut(s)	2633
BssSI	CACGAG	1 cut(s)	667
BssT1I	CCWWGG	4 cut(s)	255, 330, 1431, 2625
Bst1107I	GTATAC	1 cut(s)	2565
Bst2BI	CACGAG	1 cut(s)	667
Bst2UI	CCWGG	3 cut(s)	691, 1935, 2321
Bst6I	CTCTTC	6 cut(s)	285, 494, 730, 1346, 1879, 2205
BstACI	GRCGYC	1 cut(s)	2633
BstAUI	TGTACA	2 cut(s)	900, 2472
BstBI	TTCGAA	1 cut(s)	2992
BstC8I	GCNNGC	6 cut(s)	928, 1441, 1602, 2646, 2650, 3040
BstDEI	CTNAG	4 cut(s)	111, 120, 579, 723
BstDSI	CCRYGG	1 cut(s)	1431
BstF5I	GGATG	3 cut(s)	2533, 2678, 2762
BstFNI	CGCG	1 cut(s)	2786
BstHHI	GCGC	2 cut(s)	1617, 2788
BstKTI	GATC	8 cut(s)	176, 1270, 1810, 2265, 2331, 2549, 2560, 2715
BstMAI	GTCTC	3 cut(s)	985, 1109, 2189
BstMBI	GATC	8 cut(s)	173, 1267, 1807, 2262, 2328, 2546, 2557, 2712
BstNI	CCWGG	3 cut(s)	691, 1935, 2321
BstNSI	RCATGY	1 cut(s)	1001
BstSCI	CCNGG	3 cut(s)	689, 1933, 2319
BstUI	CGCG	1 cut(s)	2786
BstV1I	GCAGC	5 cut(s)	811, 931, 1345, 1732, 2323
BstX2I	RGATCY	1 cut(s)	1807
BstXI	CCANNNNNNTGG	1 cut(s)	713
BstYI	RGATCY	1 cut(s)	1807
BstZ17I	GTATAC	1 cut(s)	2565
BsuI	GTATCC	1 cut(s)	1226
BsuRI	GGCC	3 cut(s)	1421, 1443, 2624
BtgI	CCRYGG	1 cut(s)	1431
BtrI	CACGTC	2 cut(s)	841, 1447
BtsCI	GGATG	3 cut(s)	2533, 2678, 2762
BtsI	GCAGTG	1 cut(s)	219
BtsIMutI	CAGTG	9 cut(s)	190, 219, 341, 1320, 1785, 1972, 2618, 2968, 2983
Cac8I	GCNNGC	6 cut(s)	928, 1441, 1602, 2646, 2650, 3040
CciI	TCATGA	3 cut(s)	391, 1726, 3095
CfoI	GCGC	2 cut(s)	1617, 2788
Cfr13I	GGNCC	4 cut(s)	693, 1441, 1770, 2861
CseI	GACGC	3 cut(s)	112, 347, 2622
Csp6I	GTAC	7 cut(s)	869, 901, 1378, 1646, 2247, 2473, 2800
CspCI	CAANNNNNGTGG	2 cut(s)	2440, 2475
CviQI	GTAC	7 cut(s)	869, 901, 1378, 1646, 2247, 2473, 2800
DdeI	CTNAG	4 cut(s)	111, 120, 579, 723
DpnI	GATC	8 cut(s)	175, 1269, 1809, 2264, 2330, 2548, 2559, 2714
DpnII	GATC	8 cut(s)	173, 1267, 1807, 2262, 2328, 2546, 2557, 2712
DraI	TTTAAA	1 cut(s)	511
DraIII	CACNNNGTG	1 cut(s)	821
EaeI	YGGCCR	2 cut(s)	1419, 2622
Eam1104I	CTCTTC	6 cut(s)	285, 494, 730, 1346, 1879, 2205
EarI	CTCTTC	6 cut(s)	285, 494, 730, 1346, 1879, 2205
EciI	GGCGGA	3 cut(s)	4, 431, 1439
Ecl136II	GAGCTC	2 cut(s)	42, 1075
Eco130I	CCWWGG	4 cut(s)	255, 330, 1431, 2625
Eco24I	GRGCYC	3 cut(s)	44, 1077, 2485
Eco31I	GGTCTC	1 cut(s)	2189
Eco32I	GATATC	1 cut(s)	892
Eco47I	GGWCC	3 cut(s)	693, 1770, 2861
Eco53kI	GAGCTC	2 cut(s)	42, 1075
Eco57I	CTGAAG	6 cut(s)	518, 1040, 1488, 1601, 2186, 2844
EcoICRI	GAGCTC	2 cut(s)	42, 1075
EcoRI	GAATTC	1 cut(s)	1819
EcoRII	CCWGG	3 cut(s)	689, 1933, 2319
EcoRV	GATATC	1 cut(s)	892
EcoT14I	CCWWGG	4 cut(s)	255, 330, 1431, 2625
EcoT22I	ATGCAT	3 cut(s)	1756, 2423, 2940
EcoT38I	GRGCYC	3 cut(s)	44, 1077, 2485
ErhI	CCWWGG	4 cut(s)	255, 330, 1431, 2625
FalI	AAGNNNNNCTT	2 cut(s)	491, 523
FauI	CCCGC	2 cut(s)	1432, 1593
FauNDI	CATATG	2 cut(s)	1596, 1653
FbaI	TGATCA	1 cut(s)	2262
FblI	GTMKAC	1 cut(s)	2564
Fnu4HI	GCNGC	6 cut(s)	518, 825, 945, 1334, 1721, 2337
FokI	GGATG	3 cut(s)	2540, 2665, 2769
FriOI	GRGCYC	3 cut(s)	44, 1077, 2485
Fsp4HI	GCNGC	6 cut(s)	518, 825, 945, 1334, 1721, 2337
FspAI	RTGCGCAY	1 cut(s)	1616
FspBI	CTAG	4 cut(s)	927, 1257, 1758, 2412
FspI	TGCGCA	1 cut(s)	1616
GlaI	GCGC	2 cut(s)	1616, 2787
GluI	GCNGC	6 cut(s)	518, 825, 945, 1334, 1721, 2337
GsaI	CCCAGC	3 cut(s)	165, 612, 2499
GsuI	CTGGAG	1 cut(s)	1917
HaeIII	GGCC	3 cut(s)	1421, 1443, 2624
HapII	CCGG	1 cut(s)	2870
HgaI	GACGC	3 cut(s)	112, 347, 2622
HhaI	GCGC	2 cut(s)	1617, 2788
Hin1I	GRCGYC	1 cut(s)	2633
Hin6I	GCGC	2 cut(s)	1615, 2786
HinP1I	GCGC	2 cut(s)	1615, 2786
HincII	GTYRAC	2 cut(s)	337, 487
HindII	GTYRAC	2 cut(s)	337, 487
HindIII	AAGCTT	3 cut(s)	1053, 2511, 2826
HinfI	GANTC	9 cut(s)	473, 785, 978, 988, 1044, 1576, 1938, 1960, 2426
HpaII	CCGG	1 cut(s)	2870
HphI	GGTGA	3 cut(s)	290, 559, 1135
Hpy166II	GTNNAC	6 cut(s)	337, 487, 815, 2565, 2603, 2762
Hpy8I	GTNNAC	6 cut(s)	337, 487, 815, 2565, 2603, 2762
Hpy99I	CGWCG	3 cut(s)	106, 842, 2770
HpyCH4IV	ACGT	5 cut(s)	811, 840, 1446, 2373, 3079
HpyF3I	CTNAG	4 cut(s)	111, 120, 579, 723
HpySE526I	ACGT	5 cut(s)	811, 840, 1446, 2373, 3079
Hsp92I	GRCGYC	1 cut(s)	2633
HspAI	GCGC	2 cut(s)	1615, 2786
Ksp22I	TGATCA	1 cut(s)	2262
Kzo9I	GATC	8 cut(s)	173, 1267, 1807, 2262, 2328, 2546, 2557, 2712
LguI	GCTCTTC	2 cut(s)	730, 2205
LmnI	GCTCC	4 cut(s)	39, 442, 1546, 2574
Lsp1109I	GCAGC	5 cut(s)	811, 931, 1345, 1732, 2323
MaeI	CTAG	4 cut(s)	927, 1257, 1758, 2412
MaeII	ACGT	5 cut(s)	811, 840, 1446, 2373, 3079
MaeIII	GTNAC	6 cut(s)	217, 397, 569, 1423, 2502, 2773
MalI	GATC	8 cut(s)	175, 1269, 1809, 2264, 2330, 2548, 2559, 2714
MbiI	CCGCTC	1 cut(s)	445
MboI	GATC	8 cut(s)	173, 1267, 1807, 2262, 2328, 2546, 2557, 2712
MfeI	CAATTG	1 cut(s)	1636
MflI	RGATCY	1 cut(s)	1807
MhlI	GDGCHC	4 cut(s)	44, 1077, 1264, 2485
MlsI	TGGCCA	1 cut(s)	2624
MluNI	TGGCCA	1 cut(s)	2624
MlyI	GAGTC	3 cut(s)	779, 987, 1932
MmeI	TCCRAC	3 cut(s)	17, 712, 1764
Mox20I	TGGCCA	1 cut(s)	2624
Mph1103I	ATGCAT	3 cut(s)	1756, 2423, 2940
MroXI	GAANNNNTTC	5 cut(s)	264, 1025, 1065, 2081, 2102
MscI	TGGCCA	1 cut(s)	2624
MslI	CAYNNNNRTG	3 cut(s)	2807, 2813, 3047
Msp20I	TGGCCA	1 cut(s)	2624
MspI	CCGG	1 cut(s)	2870
MspR9I	CCNGG	3 cut(s)	691, 1935, 2321
MunI	CAATTG	1 cut(s)	1636
MvaI	CCWGG	3 cut(s)	691, 1935, 2321
MvnI	CGCG	1 cut(s)	2786
NcoI	CCATGG	1 cut(s)	1431
NdeI	CATATG	2 cut(s)	1596, 1653
NdeII	GATC	8 cut(s)	173, 1267, 1807, 2262, 2328, 2546, 2557, 2712
NheI	GCTAGC	1 cut(s)	926
NlaIV	GGNNCC	2 cut(s)	1809, 2099
NmuCI	GTSAC	2 cut(s)	217, 569
NsbI	TGCGCA	1 cut(s)	1616
NsiI	ATGCAT	3 cut(s)	1756, 2423, 2940
NspI	RCATGY	1 cut(s)	1001
NspV	TTCGAA	1 cut(s)	2992
PagI	TCATGA	3 cut(s)	391, 1726, 3095
PciI	ACATGT	1 cut(s)	997
PciSI	GCTCTTC	2 cut(s)	730, 2205
PcsI	WCGNNNNNNNCGW	1 cut(s)	173
PdmI	GAANNNNTTC	5 cut(s)	264, 1025, 1065, 2081, 2102
PfeI	GAWTC	6 cut(s)	473, 988, 1044, 1576, 1960, 2426
PfoI	TCCNGGA	2 cut(s)	1933, 2319
PkrI	GCNGC	6 cut(s)	519, 826, 946, 1335, 1722, 2338
PleI	GAGTC	3 cut(s)	779, 986, 1932
PpsI	GAGTC	3 cut(s)	779, 986, 1932
PscI	ACATGT	1 cut(s)	997
PsiI	TTATAA	2 cut(s)	588, 1713
Psp124BI	GAGCTC	2 cut(s)	44, 1077
Psp6I	CCWGG	3 cut(s)	689, 1933, 2319
PspFI	CCCAGC	3 cut(s)	161, 608, 2495
PspGI	CCWGG	3 cut(s)	689, 1933, 2319
PspN4I	GGNNCC	2 cut(s)	1809, 2099
PspPI	GGNCC	4 cut(s)	693, 1441, 1770, 2861
PsuI	RGATCY	1 cut(s)	1807
RsaI	GTAC	7 cut(s)	870, 902, 1379, 1647, 2248, 2474, 2801
RsaNI	GTAC	7 cut(s)	869, 901, 1378, 1646, 2247, 2473, 2800
RseI	CAYNNNNRTG	3 cut(s)	2807, 2813, 3047
SacI	GAGCTC	2 cut(s)	44, 1077
SapI	GCTCTTC	2 cut(s)	730, 2205
SatI	GCNGC	6 cut(s)	518, 825, 945, 1334, 1721, 2337
Sau3AI	GATC	8 cut(s)	173, 1267, 1807, 2262, 2328, 2546, 2557, 2712
Sau96I	GGNCC	4 cut(s)	693, 1441, 1770, 2861
SchI	GAGTC	3 cut(s)	779, 987, 1932
ScrFI	CCNGG	3 cut(s)	691, 1935, 2321
SduI	GDGCHC	4 cut(s)	44, 1077, 1264, 2485
SfuI	TTCGAA	1 cut(s)	2992
SinI	GGWCC	3 cut(s)	693, 1770, 2861
SmiMI	CAYNNNNRTG	3 cut(s)	2807, 2813, 3047
SmlI	CTYRAG	2 cut(s)	1076, 1237
SmoI	CTYRAG	2 cut(s)	1076, 1237
SspI	AATATT	2 cut(s)	1109, 2316
SspMI	CTAG	4 cut(s)	927, 1257, 1758, 2412
SstI	GAGCTC	2 cut(s)	44, 1077
StyD4I	CCNGG	3 cut(s)	689, 1933, 2319
StyI	CCWWGG	4 cut(s)	255, 330, 1431, 2625
TaiI	ACGT	5 cut(s)	814, 843, 1449, 2376, 3082
TaqI	TCGA	5 cut(s)	434, 2176, 2290, 2491, 2992
TatI	WGTACW	3 cut(s)	900, 1645, 2472
TauI	GCSGC	1 cut(s)	520
TfiI	GAWTC	6 cut(s)	473, 988, 1044, 1576, 1960, 2426
TscAI	CASTG	9 cut(s)	190, 226, 348, 1327, 1785, 1972, 2625, 2968, 2983
TseFI	GTSAC	2 cut(s)	217, 569
TseI	GCWGC	5 cut(s)	824, 944, 1333, 1720, 2336
Tsp45I	GTSAC	2 cut(s)	217, 569
TspGWI	ACGGA	3 cut(s)	948, 2321, 3021
TspRI	CASTG	9 cut(s)	190, 226, 348, 1327, 1785, 1972, 2625, 2968, 2983
VpaK11BI	GGWCC	3 cut(s)	693, 1770, 2861
XapI	RAATTY	8 cut(s)	77, 851, 1557, 1819, 1854, 1863, 1950, 2730
XbaI	TCTAGA	1 cut(s)	1256
XceI	RCATGY	1 cut(s)	1001
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1780
XmiI	GTMKAC	1 cut(s)	2564
XmnI	GAANNNNTTC	5 cut(s)	264, 1025, 1065, 2081, 2102
XspI	CTAG	4 cut(s)	927, 1257, 1758, 2412
Zsp2I	ATGCAT	3 cut(s)	1756, 2423, 2940

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.