Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3A

Orthogroup ID

OG0005400

Physical Location & Seq

Genomic Coordinates Reverse (-)

30843652 .. 30850219

Gene Structure 6568 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3AG270700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3072 bp

ATGGCTCTGGTGAAGCCCCATTCCCTCTCACCTCTCTCTTCCAACTCCCGCCATGCCCTCCCCCTCAACAACTCCCACTTCCGCACTTCTTTCTTCGCCGGAAAACCCTTCCGACTGCCGGAAACCACACGGATTTCGAGCCGGAGACGCCGGCGAGTGCAGGCAGTGGCTTCCCTGGGAGGTTTGTTGGGCGGGATTTTCAAAGGGACTGACACCGGTGAGTCCACCAGGCAGCAGTACGCTCAGACTGTGTCTCTGATCAACGGCTTGGAAGCTCAGATGTCTAAGCTTTCCGATTCCGAGCTCAGGGAGAAGACTCTTCAGTTTCAACAACGTGCCAAGCAGGGCGAGTCCTTGGACTCTCTTTTACCCGAAGCATTTGCTGTGATCAGAGAGGCTTCGAGGAGGGTTTTAGGCCTTCGTCCCTTTGACGTCCAACTCATAGGAGGCATAGTTCTTCACAAGGGGGAAATAGCTGAAATGAGGACTGGAGAAGGAAAGACCCTTGTTGCTATTTTACCAGCGTATTTGAATGCATTAACTGGAAAGGGAGTTCATGTTGTCACCGTCAATGATTATCTGGCCCGACGTGATTGTGAGTGGGTTGGTCAAGTTCCTCGTTTCCTTGGATTGAAGGTTGGCCTAATCCAACAGAATATGACAAGTGAACAAAGAAGGGAGAACTACTTATGTGACATCACATATGTCACAAACAGCGAGCTTGGTTTTGATTTCCTGAGAGATAATCTGGCCACGGAAAGTAATTCTTGCTTTATTTTCTTTACTAATAGTGTTGAGGAGCTTGTCTTGAGGAATTTCAATTACTGCGTAATTGATGAGGTTGATTCCATCCTTATTGATGAAGCAAGAACACCTCTCATTATATCTGGACCTGCAGAAAAACCTAGCGATAGGTATTATAAAGCTGCAAAGATGGCTTCAGTGTTTGAGCGAGATATACATTATACTGTGGATGAAAAGCAGAAAACAGTTCTGCTTTCAGAACAAGGATATGAAGATGCTGAAGAGATTCTAGGTGTAAAAGATTTATATGATCCCCGCGAACAGTGGGCATCTTATGTTCTGAATGCCGTGAAAGCAAAAGAACTGTTTCTTAGAGATGTAAATTATATAATTCGTGGCAAAGAGGTTCTTATCGTTGATGAGTTTACAGGACGAGTTATGCAGGGGAGGCGGTGGAGTGATGGACTTCACCAAGCAGTTGAAGCAAAAGAAGGTCTGCCGATTCAAAACGAAACTGTAACTCTGGCATCTATTAGCTACCAAAACTTCTTTCTCCAGTTTCCAAAACTTTGTGGAATGACTGGAACTGCAGCAACTGAAAGCACAGAATTTGAAAGCATATACAAGCTTAAAGTTACAATTGTCCCCACAAACAAGCCTATGATAAGAAAGGATGAGTCTGATGTAGTTTTCAGGGCAGCTACAGGTAAATGGCGGGCAGTTGTAGTAGAGATTTCTAGAATGCACAAAACAGGTCGCCCTGTACTTGTTGGCACAACTAGTGTTGAGCAGAGTGACTCATTGTCTGAGCAGTTGCAAGAAGTTGGCATTCCCCATGAGGTTCTCAACGCAAAACCAGAGAATGTGGAGAGGGAAGCAGAAATTGTGGCACAAAGCGGTCGTCTAGGGGCAGTTACTATTGCAACCAATATGGCAGGTCGAGGAACAGACATAATCCTTGGTGGGAATGCAGAATTTATGGCAAGGTTGAAGCTGCGTGAGATGCTTATGCCAAGAGTTGTAAAACTAACTGAAGGAGGTTATGTCTCAGTGAAAAAACTTCCGCCAAAAAAGTCCTGGAAGGTTAATGAAAAGCTATTTCCATGCAAACTGTCCAATGAAAAGACCAAGTTAGCTGAGGAAGCTGTGAATTTAGCTGTTGAAACTTGGGGTCAGAGATCATTGACGGAGCTTGAAGCAGAGGAGCGTCTTTCTTATTCTTGCGAAAAGGGACCTGCTTTGGATGAAGTGATAGCCAAACTGCGGAGTGCTTTTTTAGAAATCATGAAAGAATATAAGGGCTACACAGAGGAAGAAAGGAAGAAGGTTGTGTCAGCTGGTGGGCTTCATGTTGTCGGGACAGAACGCCATGAATCTCGCCGAGTTGATAATCAGTTACGTGGTCGAACTGGACGGCAGGGGGATCCTGGAAGTTCACGCTTCTTCTTAAGTCTTGAAGATAACATCTTTCGTATATTTGGTGGAGATCGTATTCAGGGTTTAATGAGAGCTTTTAGAGTTGAGGACTTGCCAATTGAGTCCAAGATGCTGACCAAAGCACTAGACGAAGCTCAGCGGAAAGTGGAAAACTATTTTTTCGATATTCGAAAGCAATTGTTTGAATTTGATGAAGTCCTGAACAGCCAAAGAGACCGTGTGTATACGGAAAGAAGGAGAGCCCTTGAATCTGACAATCTGCAATCACTTATAATTGAATATGCTGAACTAACAATGGATGATATCTTAGAGGCAAATATTGGTTCAGAGTCTTCAAAAGAAAGCTGGGATCTTGATAAGCTCATCAAGAAACTTCAACAATATTGCTATCTATTGAATGATTTGACGCCGGATGTGCTGAGGAGCGAATGTTCAAGTTATGAAGATTTGCAGGACTACCTCCGTCTTCGAGGTCGTGAAGCATATTTGCAGAAAAGGACTATTATCGAGAGCCAGGCACCGGGCTTGATGAAGGACGCTGAACGGTTCTTAGTTTTGAACAATATTGACCGATTGTGGAAGGAGCACTTACAAGCACTCAAGTTCGTTCAGCAAGCTGTTGGTTTGCGTGGGTACGCACAACGGGATCCACTTATTGAATATAAGCTTGAAGGCTACAATCTTTTCTTGGACATGATGGCACAGATAAGAAGAAATGTTATATATTCCATATACCAGTTTCAACCTGTGATGGTAAAGAAGGATGGGGATCAAAGAGAGAATAAGAAATCAGGAAAACTTGTCACTAATGGTAGCGGTAATGGTAACCCCAATTCAGTTGGCGGTGTTGAGTCTTCTTCAGCAGCTGCCACCCCCCAATCTAGTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005524 GO:0006810 GO:0008104 GO:0008144 GO:0008150 GO:0008320 GO:0008565 GO:0015031 GO:0015399 GO:0015405 GO:0015440 GO:0015450 GO:0015462 GO:0015833 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022804 GO:0022857 GO:0022884 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0033036 GO:0033220 GO:0035639 GO:0036094 GO:0042623 GO:0042626 GO:0042886 GO:0042887 GO:0043167 GO:0043168 GO:0043492 GO:0045184 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0071806 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1904680

KEGG Pathways

Metabolic & Signaling

ko02024 ko03060 ko03070 map02024 map03060 map03070

Pfam Domains

Protein Families

SecA_DEAD SecA_PP_bind SecA_SW

Protein Analysis

1023

Amino Acids

115.69

Weight (kDa)

6.92

Isoelectric Point (pI)

46.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SecA_DEAD	PF07517	74 - 453	6.7e-123	SecA DEAD-like domain
DEAD	PF00270	158 - 286	1.7e-06	DEAD/DEAH box helicase
SecA_PP_bind	PF01043	303 - 409	1e-37	SecA preprotein cross-linking domain
P-loop_SecA	PF21090	468 - 757	1.6e-82	SecA P-loop domain
SecA_SW	PF07516	759 - 975	2.9e-56	SecA Wing and Scaffold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005400)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G01800 AT4G01800 AT4G01800
fragaria_vesca	FvH4_1g26240
malus_domestica	MD17G1285000.v1.1
prunus_persica	Prupe.3G002700_v2.0.a1
pyrus_communis	pycom09g19850 pycom17g28080 pycom17g28090
rosa_chinensis	RchiOBHm_Chr3g0485781
rosa_laevigata	RLG00000023125
rosa_multiflora	Rmu_co8471925.1_g000001 Rmu_sc0012870.1_g000002
rosa_roxburghii	Rroxscaffold_2G00123840
rosa_rugosa	Rorug03G0221400
rosa_samantha	Rh1AG180100 Rh1CG167100 Rh3AG270700 Rh3BG306200 Rh3BG306300 Rh3CG304600 Rh3DG300700 Rh4AG241300 Rh4AG241400 Rh4BG244600 Rh4BG244700 Rh4CG255400 Rh7CG119700
rosa_wichuraiana	Rw3G024110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	921, 2453
AatII	GACGTC	1 cut(s)	435
Acc36I	ACCTGC	3 cut(s)	901, 1670, 1987
AccB1I	GGYRCC	1 cut(s)	2698
AccI	GTMKAC	1 cut(s)	2405
AccII	CGCG	1 cut(s)	1062
AclWI	GGATC	7 cut(s)	1049, 2162, 2175, 2538, 2822, 2835, 2958
AcoI	YGGCCR	1 cut(s)	750
AcsI	RAATTY	5 cut(s)	814, 1352, 1718, 1894, 2366
AcuI	CTGAAG	5 cut(s)	305, 924, 1044, 1797, 3024
AcyI	GRCGYC	3 cut(s)	148, 432, 2588
AfaI	GTAC	3 cut(s)	239, 1509, 2816
AfiI	CCNNNNNNNGG	4 cut(s)	118, 306, 2007, 2701
AflII	CTTAAG	1 cut(s)	2191
AgeI	ACCGGT	1 cut(s)	215
AhdI	GACNNNNNGTC	1 cut(s)	1546
AhlI	ACTAGT	1 cut(s)	1523
AjiI	CACGTC	1 cut(s)	590
AjnI	CCWGG	5 cut(s)	174, 227, 1820, 2170, 2694
AjuI	GAANNNNNNNTTGG	2 cut(s)	1278, 1310
AloI	GAACNNNNNNTCC	6 cut(s)	438, 470, 537, 569, 2596, 2628
Alw21I	GWGCWC	2 cut(s)	306, 2769
Alw26I	GTCTC	4 cut(s)	139, 258, 1795, 2388
AlwI	GGATC	7 cut(s)	1049, 2162, 2175, 2538, 2822, 2835, 2958
AlwNI	CAGNNNCTG	2 cut(s)	1340, 3047
AoxI	GGCC	4 cut(s)	415, 582, 640, 750
ApeKI	GCWGC	7 cut(s)	232, 926, 1334, 1442, 1738, 3044, 3047
ApoI	RAATTY	5 cut(s)	814, 1352, 1718, 1894, 2366
ArsI	GACNNNNNNTTYG	2 cut(s)	1630, 1662
AsiGI	ACCGGT	1 cut(s)	215
Asp700I	GAANNNNTTC	2 cut(s)	1029, 1110
AspS9I	GGNCC	3 cut(s)	583, 890, 1976
AsuC2I	CCSGG	1 cut(s)	2703
AsuHPI	GGTGA	5 cut(s)	21, 22, 230, 556, 1205
AsuII	TTCGAA	1 cut(s)	2350
AvaII	GGWCC	2 cut(s)	890, 1976
BalI	TGGCCA	1 cut(s)	752
BamHI	GGATCC	2 cut(s)	2167, 2827
BanI	GGYRCC	1 cut(s)	2698
BanII	GRGCYC	2 cut(s)	306, 2425
BbsI	GAAGAC	4 cut(s)	320, 2505, 2639, 3027
Bbv12I	GWGCWC	2 cut(s)	306, 2769
BbvCI	CCTCAGC	2 cut(s)	1881, 2600
BbvI	GCAGC	7 cut(s)	244, 913, 1346, 1454, 1725, 3034, 3056
BccI	CCATC	6 cut(s)	857, 928, 1199, 2872, 2926, 2939
BceAI	ACGGC	3 cut(s)	280, 1076, 2174
BcgI	CGANNNNNNTGC	2 cut(s)	362, 396
BciT130I	CCWGG	5 cut(s)	176, 229, 1822, 2172, 2696
BclI	TGATCA	2 cut(s)	258, 387
BcnI	CCSGG	1 cut(s)	2703
BcoDI	GTCTC	4 cut(s)	139, 258, 1795, 2388
BcuI	ACTAGT	1 cut(s)	1523
BfaI	CTAG	7 cut(s)	906, 1034, 1482, 1524, 1649, 2306, 3063
BfmI	CTRYAG	3 cut(s)	894, 1332, 1446
BfrI	CTTAAG	1 cut(s)	2191
BfuAI	ACCTGC	3 cut(s)	901, 1670, 1987
BisI	GCNGC	7 cut(s)	233, 927, 1335, 1443, 1739, 3045, 3048
BlpI	GCTNAGC	1 cut(s)	2316
BlsI	GCNGC	7 cut(s)	234, 928, 1336, 1444, 1740, 3046, 3049
Bme1390I	CCNGG	6 cut(s)	176, 229, 1822, 2172, 2696, 2703
Bme18I	GGWCC	2 cut(s)	890, 1976
BmeRI	GACNNNNNGTC	1 cut(s)	1546
BmgBI	CACGTC	1 cut(s)	590
BmgT120I	GGNCC	3 cut(s)	583, 890, 1976
BmiI	GGNNCC	4 cut(s)	1977, 2169, 2700, 2829
BmrFI	CCNGG	6 cut(s)	176, 229, 1822, 2172, 2696, 2703
BmsI	GCATC	5 cut(s)	1009, 1082, 1280, 1737, 2280
BpiI	GAAGAC	4 cut(s)	320, 2505, 2639, 3027
BpmI	CTGGAG	2 cut(s)	510, 1283
Bpu10I	CCTNAGC	3 cut(s)	305, 1881, 2600
Bpu1102I	GCTNAGC	1 cut(s)	2316
Bpu14I	TTCGAA	1 cut(s)	2350
BpuEI	CTTGAG	2 cut(s)	829, 2765
BpuMI	CCSGG	1 cut(s)	2703
BsaAI	YACGTR	1 cut(s)	2144
BsaBI	GATNNNNATC	1 cut(s)	2949
BsaHI	GRCGYC	3 cut(s)	148, 432, 2588
BsaI	GGTCTC	1 cut(s)	2388
BsaJI	CCNNGG	6 cut(s)	174, 175, 354, 625, 753, 1702
BsaWI	WCCGGW	1 cut(s)	215
BsaXI	ACNNNNNCTCC	8 cut(s)	438, 468, 543, 573, 1773, 1803, 2596, 2626
Bsc4I	CCNNNNNNNGG	4 cut(s)	118, 306, 2007, 2701
Bse118I	RCCGGY	2 cut(s)	150, 215
Bse1I	ACTGG	6 cut(s)	493, 547, 1300, 1330, 2158, 2917
Bse8I	GATNNNNATC	1 cut(s)	2949
BseBI	CCWGG	5 cut(s)	176, 229, 1822, 2172, 2696
BseDI	CCNNGG	6 cut(s)	174, 175, 354, 625, 753, 1702
BseGI	GGATG	7 cut(s)	849, 979, 1423, 1993, 2485, 2599, 2950
BseJI	GATNNNNATC	1 cut(s)	2949
BseLI	CCNNNNNNNGG	4 cut(s)	118, 306, 2007, 2701
BseMII	CTCAG	9 cut(s)	257, 290, 319, 728, 1542, 1806, 1872, 2330, 2591
BseNI	ACTGG	6 cut(s)	493, 547, 1300, 1330, 2158, 2917
BseRI	GAGGAG	4 cut(s)	418, 812, 1961, 2617
BseXI	GCAGC	7 cut(s)	244, 913, 1346, 1454, 1725, 3034, 3056
BseYI	CCCAGC	1 cut(s)	2526
BsgI	GTGCAG	1 cut(s)	179
Bsh1236I	CGCG	1 cut(s)	1062
Bsh1285I	CGRYCG	1 cut(s)	1645
BshFI	GGCC	4 cut(s)	417, 584, 642, 752
BshNI	GGYRCC	1 cut(s)	2698
BshTI	ACCGGT	1 cut(s)	215
BsiEI	CGRYCG	1 cut(s)	1645
BsiHKAI	GWGCWC	2 cut(s)	306, 2769
BsiSI	CCGG	7 cut(s)	99, 119, 142, 151, 216, 2591, 2702
BslFI	GGGAC	5 cut(s)	220, 408, 1373, 1989, 2116
BslI	CCNNNNNNNGG	4 cut(s)	118, 306, 2007, 2701
BsmAI	GTCTC	4 cut(s)	139, 258, 1795, 2388
BsmBI	CGTCTC	1 cut(s)	139
BsmFI	GGGAC	5 cut(s)	220, 408, 1373, 1989, 2116
BsmI	GAATGC	5 cut(s)	538, 1093, 1491, 1572, 1717
BsnI	GGCC	4 cut(s)	417, 584, 642, 752
Bso31I	GGTCTC	1 cut(s)	2388
Bsp119I	TTCGAA	1 cut(s)	2350
Bsp1286I	GDGCHC	3 cut(s)	306, 2425, 2769
Bsp143I	GATC	9 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2827, 2950
Bsp1720I	GCTNAGC	1 cut(s)	2316
BspANI	GGCC	4 cut(s)	417, 584, 642, 752
BspCNI	CTCAG	9 cut(s)	256, 289, 318, 729, 1543, 1805, 1873, 2329, 2592
BspFNI	CGCG	1 cut(s)	1062
BspHI	TCATGA	1 cut(s)	2028
BspLI	GGNNCC	4 cut(s)	1977, 2169, 2700, 2829
BspMAI	CTGCAG	2 cut(s)	898, 1336
BspMI	ACCTGC	3 cut(s)	901, 1670, 1987
BspPI	GGATC	7 cut(s)	1049, 2162, 2175, 2538, 2822, 2835, 2958
BspT104I	TTCGAA	1 cut(s)	2350
BspT107I	GGYRCC	1 cut(s)	2698
BspTI	CTTAAG	1 cut(s)	2191
BspTNI	GGTCTC	1 cut(s)	2388
BsrFI	RCCGGY	2 cut(s)	150, 215
BsrI	ACTGG	6 cut(s)	493, 547, 1300, 1330, 2158, 2917
BssAI	RCCGGY	2 cut(s)	150, 215
BssECI	CCNNGG	6 cut(s)	174, 175, 354, 625, 753, 1702
BssMI	GATC	9 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2827, 2950
BssNAI	GTATAC	1 cut(s)	2406
BssNI	GRCGYC	3 cut(s)	148, 432, 2588
BssT1I	CCWWGG	3 cut(s)	354, 625, 1702
Bst1107I	GTATAC	1 cut(s)	2406
Bst2UI	CCWGG	5 cut(s)	176, 229, 1822, 2172, 2696
Bst6I	CTCTTC	3 cut(s)	43, 324, 1020
BstACI	GRCGYC	3 cut(s)	148, 432, 2588
BstAFI	CTTAAG	1 cut(s)	2191
BstBAI	YACGTR	1 cut(s)	2144
BstBI	TTCGAA	1 cut(s)	2350
BstC8I	GCNNGC	5 cut(s)	152, 162, 719, 1461, 2796
BstDSI	CCRYGG	1 cut(s)	753
BstEII	GGTNACC	1 cut(s)	3005
BstF5I	GGATG	7 cut(s)	849, 979, 1423, 1993, 2485, 2599, 2950
BstFNI	CGCG	1 cut(s)	1062
BstKTI	GATC	9 cut(s)	261, 390, 1057, 1925, 2170, 2233, 2533, 2830, 2953
BstMAI	GTCTC	4 cut(s)	139, 258, 1795, 2388
BstMBI	GATC	9 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2827, 2950
BstMCI	CGRYCG	1 cut(s)	1645
BstMWI	GCNNNNNNNGC	8 cut(s)	147, 935, 1097, 1192, 1226, 1747, 1886, 2596
BstNI	CCWGG	5 cut(s)	176, 229, 1822, 2172, 2696
BstPI	GGTNACC	1 cut(s)	3005
BstSCI	CCNGG	6 cut(s)	174, 227, 1820, 2170, 2694, 2701
BstSFI	CTRYAG	3 cut(s)	894, 1332, 1446
BstUI	CGCG	1 cut(s)	1062
BstV1I	GCAGC	7 cut(s)	244, 913, 1346, 1454, 1725, 3034, 3056
BstV2I	GAAGAC	4 cut(s)	320, 2505, 2639, 3027
BstX2I	RGATCY	3 cut(s)	2167, 2530, 2827
BstYI	RGATCY	3 cut(s)	2167, 2530, 2827
BstZ17I	GTATAC	1 cut(s)	2406
BsuRI	GGCC	4 cut(s)	417, 584, 642, 752
BtgI	CCRYGG	1 cut(s)	753
BtrI	CACGTC	1 cut(s)	590
BtsCI	GGATG	7 cut(s)	849, 979, 1423, 1993, 2485, 2599, 2950
BtsI	GCAGTG	1 cut(s)	171
BtsIMutI	CAGTG	4 cut(s)	171, 948, 1073, 1800
BveI	ACCTGC	3 cut(s)	901, 1670, 1987
Cac8I	GCNNGC	5 cut(s)	152, 162, 719, 1461, 2796
CaiI	CAGNNNCTG	2 cut(s)	1340, 3047
CciI	TCATGA	1 cut(s)	2028
Cfr10I	RCCGGY	2 cut(s)	150, 215
Cfr13I	GGNCC	3 cut(s)	583, 890, 1976
CseI	GACGC	4 cut(s)	156, 1940, 2596, 2726
Csp6I	GTAC	3 cut(s)	238, 1508, 2815
CspAI	ACCGGT	1 cut(s)	215
CviAII	CATG	8 cut(s)	53, 557, 1580, 1848, 2029, 2093, 2114, 2875
CviQI	GTAC	3 cut(s)	238, 1508, 2815
DpnI	GATC	9 cut(s)	260, 389, 1056, 1924, 2169, 2232, 2532, 2829, 2952
DpnII	GATC	9 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2827, 2950
DriI	GACNNNNNGTC	1 cut(s)	1546
EaeI	YGGCCR	1 cut(s)	750
Eam1104I	CTCTTC	3 cut(s)	43, 324, 1020
Eam1105I	GACNNNNNGTC	1 cut(s)	1546
EarI	CTCTTC	3 cut(s)	43, 324, 1020
EciI	GGCGGA	1 cut(s)	1797
Ecl136II	GAGCTC	1 cut(s)	304
Eco130I	CCWWGG	3 cut(s)	354, 625, 1702
Eco147I	AGGCCT	1 cut(s)	417
Eco24I	GRGCYC	2 cut(s)	306, 2425
Eco31I	GGTCTC	1 cut(s)	2388
Eco32I	GATATC	1 cut(s)	2485
Eco47I	GGWCC	2 cut(s)	890, 1976
Eco53kI	GAGCTC	1 cut(s)	304
Eco57I	CTGAAG	5 cut(s)	305, 924, 1044, 1797, 3024
Eco91I	GGTNACC	1 cut(s)	3005
EcoICRI	GAGCTC	1 cut(s)	304
EcoO109I	RGGNCCY	1 cut(s)	1976
EcoO65I	GGTNACC	1 cut(s)	3005
EcoRII	CCWGG	5 cut(s)	174, 227, 1820, 2170, 2694
EcoRV	GATATC	1 cut(s)	2485
EcoT14I	CCWWGG	3 cut(s)	354, 625, 1702
EcoT22I	ATGCAT	1 cut(s)	538
EcoT38I	GRGCYC	2 cut(s)	306, 2425
ErhI	CCWWGG	3 cut(s)	354, 625, 1702
Esp3I	CGTCTC	1 cut(s)	139
FaeI	CATG	8 cut(s)	56, 560, 1583, 1851, 2032, 2096, 2117, 2878
FalI	AAGNNNNNCTT	6 cut(s)	751, 783, 1137, 1169, 2753, 2785
FaqI	GGGAC	5 cut(s)	220, 408, 1373, 1989, 2116
FatI	CATG	8 cut(s)	52, 556, 1579, 1847, 2028, 2092, 2113, 2874
FauI	CCCGC	4 cut(s)	56, 185, 1067, 1452
FauNDI	CATATG	1 cut(s)	703
FbaI	TGATCA	2 cut(s)	258, 387
FblI	GTMKAC	1 cut(s)	2405
Fnu4HI	GCNGC	7 cut(s)	233, 927, 1335, 1443, 1739, 3045, 3048
FokI	GGATG	7 cut(s)	836, 986, 1430, 2000, 2492, 2606, 2957
FriOI	GRGCYC	2 cut(s)	306, 2425
Fsp4HI	GCNGC	7 cut(s)	233, 927, 1335, 1443, 1739, 3045, 3048
FspBI	CTAG	7 cut(s)	906, 1034, 1482, 1524, 1649, 2306, 3063
GluI	GCNGC	7 cut(s)	233, 927, 1335, 1443, 1739, 3045, 3048
GsaI	CCCAGC	1 cut(s)	2530
GsuI	CTGGAG	2 cut(s)	510, 1283
HaeIII	GGCC	4 cut(s)	417, 584, 642, 752
HapII	CCGG	7 cut(s)	99, 119, 142, 151, 216, 2591, 2702
HgaI	GACGC	4 cut(s)	156, 1940, 2596, 2726
Hin1I	GRCGYC	3 cut(s)	148, 432, 2588
Hin1II	CATG	8 cut(s)	56, 560, 1583, 1851, 2032, 2096, 2117, 2878
HindIII	AAGCTT	3 cut(s)	287, 1370, 2846
HpaII	CCGG	7 cut(s)	99, 119, 142, 151, 216, 2591, 2702
HphI	GGTGA	5 cut(s)	21, 22, 230, 556, 1205
Hpy166II	GTNNAC	5 cut(s)	225, 668, 1170, 2180, 2406
Hpy8I	GTNNAC	5 cut(s)	225, 668, 1170, 2180, 2406
Hpy99I	CGWCG	1 cut(s)	591
HpyCH4IV	ACGT	4 cut(s)	334, 432, 589, 2143
HpyF10VI	GCNNNNNNNGC	8 cut(s)	147, 935, 1097, 1192, 1226, 1747, 1886, 2596
HpySE526I	ACGT	4 cut(s)	334, 432, 589, 2143
Hsp92I	GRCGYC	3 cut(s)	148, 432, 2588
Hsp92II	CATG	8 cut(s)	56, 560, 1583, 1851, 2032, 2096, 2117, 2878
KroI	GCCGGC	1 cut(s)	150
KroNI	GCCGGC	1 cut(s)	152
Ksp22I	TGATCA	2 cut(s)	258, 387
Kzo9I	GATC	9 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2827, 2950
LmnI	GCTCC	5 cut(s)	799, 1933, 1948, 2604, 2764
Lsp1109I	GCAGC	7 cut(s)	244, 913, 1346, 1454, 1725, 3034, 3056
LweI	GCATC	5 cut(s)	1009, 1082, 1280, 1737, 2280
MaeI	CTAG	7 cut(s)	906, 1034, 1482, 1524, 1649, 2306, 3063
MaeII	ACGT	4 cut(s)	334, 432, 589, 2143
MalI	GATC	9 cut(s)	260, 389, 1056, 1924, 2169, 2232, 2532, 2829, 2952
MboI	GATC	9 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2827, 2950
MfeI	CAATTG	3 cut(s)	1383, 2277, 2357
MflI	RGATCY	3 cut(s)	2167, 2530, 2827
MhlI	GDGCHC	3 cut(s)	306, 2425, 2769
MlsI	TGGCCA	1 cut(s)	752
MluNI	TGGCCA	1 cut(s)	752
MlyI	GAGTC	9 cut(s)	230, 310, 353, 359, 1430, 1535, 2291, 2519, 3041
MmeI	TCCRAC	4 cut(s)	66, 136, 460, 673
Mox20I	TGGCCA	1 cut(s)	752
Mph1103I	ATGCAT	1 cut(s)	538
MreI	CGCCGGCG	1 cut(s)	150
MroNI	GCCGGC	1 cut(s)	150
MroXI	GAANNNNTTC	2 cut(s)	1029, 1110
MscI	TGGCCA	1 cut(s)	752
MseI	TTAA	5 cut(s)	539, 1374, 1830, 2192, 2246
Msp20I	TGGCCA	1 cut(s)	752
MspA1I	CMGCKG	3 cut(s)	2081, 2320, 3047
MspCI	CTTAAG	1 cut(s)	2191
MspI	CCGG	7 cut(s)	99, 119, 142, 151, 216, 2591, 2702
MspR9I	CCNGG	6 cut(s)	176, 229, 1822, 2172, 2696, 2703
MunI	CAATTG	3 cut(s)	1383, 2277, 2357
Mva1269I	GAATGC	5 cut(s)	538, 1093, 1491, 1572, 1717
MvaI	CCWGG	5 cut(s)	176, 229, 1822, 2172, 2696
MvnI	CGCG	1 cut(s)	1062
MwoI	GCNNNNNNNGC	8 cut(s)	147, 935, 1097, 1192, 1226, 1747, 1886, 2596
NaeI	GCCGGC	1 cut(s)	152
NciI	CCSGG	1 cut(s)	2703
NdeI	CATATG	1 cut(s)	703
NdeII	GATC	9 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2827, 2950
NgoMIV	GCCGGC	1 cut(s)	150
NlaIII	CATG	8 cut(s)	56, 560, 1583, 1851, 2032, 2096, 2117, 2878
NlaIV	GGNNCC	4 cut(s)	1977, 2169, 2700, 2829
NmeAIII	GCCGAG	1 cut(s)	2150
NmuCI	GTSAC	5 cut(s)	562, 692, 706, 1538, 2983
NsiI	ATGCAT	1 cut(s)	538
NspV	TTCGAA	1 cut(s)	2350
PagI	TCATGA	1 cut(s)	2028
PasI	CCCWGGG	1 cut(s)	175
PceI	AGGCCT	1 cut(s)	417
PctI	GAATGC	5 cut(s)	538, 1093, 1491, 1572, 1717
PdiI	GCCGGC	1 cut(s)	152
PdmI	GAANNNNTTC	2 cut(s)	1029, 1110
PfeI	GAWTC	6 cut(s)	296, 845, 1030, 1246, 2117, 2429
PflFI	GACNNNGTC	1 cut(s)	250
PfoI	TCCNGGA	2 cut(s)	1820, 2170
PinAI	ACCGGT	1 cut(s)	215
PkrI	GCNGC	7 cut(s)	234, 928, 1336, 1444, 1740, 3046, 3049
PleI	GAGTC	9 cut(s)	229, 310, 353, 358, 1429, 1535, 2290, 2518, 3040
PpsI	GAGTC	9 cut(s)	229, 310, 353, 358, 1429, 1535, 2290, 2518, 3040
Ppu21I	YACGTR	1 cut(s)	2144
PpuMI	RGGWCCY	1 cut(s)	1976
PsiI	TTATAA	2 cut(s)	921, 2453
Psp124BI	GAGCTC	1 cut(s)	306
Psp5II	RGGWCCY	1 cut(s)	1976
Psp6I	CCWGG	5 cut(s)	174, 227, 1820, 2170, 2694
PspEI	GGTNACC	1 cut(s)	3005
PspFI	CCCAGC	1 cut(s)	2526
PspGI	CCWGG	5 cut(s)	174, 227, 1820, 2170, 2694
PspN4I	GGNNCC	4 cut(s)	1977, 2169, 2700, 2829
PspPI	GGNCC	3 cut(s)	583, 890, 1976
PspPPI	RGGWCCY	1 cut(s)	1976
PstI	CTGCAG	2 cut(s)	898, 1336
PstNI	CAGNNNCTG	2 cut(s)	1340, 3047
PsuI	RGATCY	3 cut(s)	2167, 2530, 2827
PsyI	GACNNNGTC	1 cut(s)	250
PvuII	CAGCTG	2 cut(s)	2081, 3047
RsaI	GTAC	3 cut(s)	239, 1509, 2816
RsaNI	GTAC	3 cut(s)	238, 1508, 2815
SacI	GAGCTC	1 cut(s)	306
SaqAI	TTAA	5 cut(s)	539, 1374, 1830, 2192, 2246
SatI	GCNGC	7 cut(s)	233, 927, 1335, 1443, 1739, 3045, 3048
Sau3AI	GATC	9 cut(s)	258, 387, 1054, 1922, 2167, 2230, 2530, 2827, 2950
Sau96I	GGNCC	3 cut(s)	583, 890, 1976
SchI	GAGTC	9 cut(s)	230, 310, 353, 359, 1430, 1535, 2291, 2519, 3041
ScrFI	CCNGG	6 cut(s)	176, 229, 1822, 2172, 2696, 2703
SduI	GDGCHC	3 cut(s)	306, 2425, 2769
SfaNI	GCATC	5 cut(s)	1009, 1082, 1280, 1737, 2280
SfcI	CTRYAG	3 cut(s)	894, 1332, 1446
SfuI	TTCGAA	1 cut(s)	2350
SgrAI	CRCCGGYG	2 cut(s)	150, 215
SinI	GGWCC	2 cut(s)	890, 1976
SmlI	CTYRAG	3 cut(s)	808, 2191, 2780
SmoI	CTYRAG	3 cut(s)	808, 2191, 2780
SpeI	ACTAGT	1 cut(s)	1523
SseBI	AGGCCT	1 cut(s)	417
SspI	AATATT	3 cut(s)	2500, 2564, 2746
SspMI	CTAG	7 cut(s)	906, 1034, 1482, 1524, 1649, 2306, 3063
SstI	GAGCTC	1 cut(s)	306
StuI	AGGCCT	1 cut(s)	417
StyD4I	CCNGG	6 cut(s)	174, 227, 1820, 2170, 2694, 2701
StyI	CCWWGG	3 cut(s)	354, 625, 1702
TaiI	ACGT	4 cut(s)	337, 435, 592, 2146
TaqI	TCGA	8 cut(s)	137, 401, 1684, 2149, 2343, 2350, 2650, 2688
TaqII	GACCGA	1 cut(s)	2766
TatI	WGTACW	1 cut(s)	1507
TfiI	GAWTC	6 cut(s)	296, 845, 1030, 1246, 2117, 2429
Tru1I	TTAA	5 cut(s)	539, 1374, 1830, 2192, 2246
Tru9I	TTAA	5 cut(s)	539, 1374, 1830, 2192, 2246
TscAI	CASTG	4 cut(s)	171, 948, 1073, 1800
TseFI	GTSAC	5 cut(s)	562, 692, 706, 1538, 2983
TseI	GCWGC	7 cut(s)	232, 926, 1334, 1442, 1738, 3044, 3047
Tsp45I	GTSAC	5 cut(s)	562, 692, 706, 1538, 2983
TspGWI	ACGGA	5 cut(s)	145, 770, 1946, 2423, 2633
TspRI	CASTG	4 cut(s)	171, 948, 1073, 1800
Tth111I	GACNNNGTC	1 cut(s)	250
Vha464I	CTTAAG	1 cut(s)	2191
VpaK11BI	GGWCC	2 cut(s)	890, 1976
XapI	RAATTY	5 cut(s)	814, 1352, 1718, 1894, 2366
XbaI	TCTAGA	1 cut(s)	1481
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1818
XmiI	GTMKAC	1 cut(s)	2405
XmnI	GAANNNNTTC	2 cut(s)	1029, 1110
XspI	CTAG	7 cut(s)	906, 1034, 1482, 1524, 1649, 2306, 3063
ZraI	GACGTC	1 cut(s)	433
Zsp2I	ATGCAT	1 cut(s)	538

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.